Carg06640 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg06640
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionmitogen-activated protein kinase kinase 9-like
LocationCarg_Chr18: 394422 .. 395354 (-)
RNA-Seq ExpressionCarg06640
SyntenyCarg06640
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCTGGTTCGTGATCGCCTCCAGTTGAAACTCCGCCTCCCGGACCTCTCCGACTGCGGTCCCCGCTTCCCCCTCCCCCTCCCACCCTCCTCTGCCCCTGCCATCTCCTCCGCCGACCTCGAAAAGCTCCAAGTCCTCGGGCACGGCAACGGTGGCACAGTCTACAAAGTCCGCCACAAGCGAACCTCCAACATCTACGCTCTCAAGGTCGTCCACGGCGACGGCGATCCCACCGTTCGCCGCCAGGTTTTCAGAGAGATGGAGATTCTCCGCCGTACGGACTCTCCTTACGTCGTGAAATGCCATGGAATCTTCGAGAAGCCGTCTGGAGATGTCACGATTTTGATGGAGTATATGGATCGAGGATCGCTTGATACGCTCTTGAAGAAGAATACCACGCTGTCAGAGTCCACGCTCGCTCACGTATCGCGTCAGGTTCTTAATGGCCTTCACTACCTTCACTCTCATAAAATCATTCATCGCGATATTAAACCGTCGAATCTCTTGGTGAATAAGAATATGGAGGTTAAGATTGCCGATTTTGGAGTTAGTAAAATCATGTGTAGGACTCTGGATGCTTGCAATTCCTACGTCGGAACCTGCGCTTATATGAGTCCGGAGCGGTTCGATCCAGAGACTTACGGCGGAAATTACAACGGTTACGCCGGAGACATATGGAGTTTAGGCCTTACTCTTCTGGAACTCTATTTAGGCCATTTTCCTTTTCTTCCGCGTGGTCAGAGACCGGATTGGGCGACTCTGATGTTTGCGATTTGCTTTGATGAGCCGCCGACGCTGCCGGAGGATGCGTCAGAGGAGTTTCGAAGCTTCGTCCAGAGTTGTTTGCAGAAGGAATCGAGCAAGAGATGGACGGCGGCGCAATTATTAACGCATCCGTTTGTAAGTAAGGAATCGAGATCCGATCGGTGA

mRNA sequence

ATGGCTCTGGTTCGTGATCGCCTCCAGTTGAAACTCCGCCTCCCGGACCTCTCCGACTGCGGTCCCCGCTTCCCCCTCCCCCTCCCACCCTCCTCTGCCCCTGCCATCTCCTCCGCCGACCTCGAAAAGCTCCAAGTCCTCGGGCACGGCAACGGTGGCACAGTCTACAAAGTCCGCCACAAGCGAACCTCCAACATCTACGCTCTCAAGGTCGTCCACGGCGACGGCGATCCCACCGTTCGCCGCCAGGTTTTCAGAGAGATGGAGATTCTCCGCCGTACGGACTCTCCTTACGTCGTGAAATGCCATGGAATCTTCGAGAAGCCGTCTGGAGATGTCACGATTTTGATGGAGTATATGGATCGAGGATCGCTTGATACGCTCTTGAAGAAGAATACCACGCTGTCAGAGTCCACGCTCGCTCACGTATCGCGTCAGGTTCTTAATGGCCTTCACTACCTTCACTCTCATAAAATCATTCATCGCGATATTAAACCGTCGAATCTCTTGGTGAATAAGAATATGGAGGTTAAGATTGCCGATTTTGGAGTTAGTAAAATCATGTGTAGGACTCTGGATGCTTGCAATTCCTACGTCGGAACCTGCGCTTATATGAGTCCGGAGCGGTTCGATCCAGAGACTTACGGCGGAAATTACAACGGTTACGCCGGAGACATATGGAGTTTAGGCCTTACTCTTCTGGAACTCTATTTAGGCCATTTTCCTTTTCTTCCGCGTGGTCAGAGACCGGATTGGGCGACTCTGATGTTTGCGATTTGCTTTGATGAGCCGCCGACGCTGCCGGAGGATGCGTCAGAGGAGTTTCGAAGCTTCGTCCAGAGTTGTTTGCAGAAGGAATCGAGCAAGAGATGGACGGCGGCGCAATTATTAACGCATCCGTTTGTAAGTAAGGAATCGAGATCCGATCGGTGA

Coding sequence (CDS)

ATGGCTCTGGTTCGTGATCGCCTCCAGTTGAAACTCCGCCTCCCGGACCTCTCCGACTGCGGTCCCCGCTTCCCCCTCCCCCTCCCACCCTCCTCTGCCCCTGCCATCTCCTCCGCCGACCTCGAAAAGCTCCAAGTCCTCGGGCACGGCAACGGTGGCACAGTCTACAAAGTCCGCCACAAGCGAACCTCCAACATCTACGCTCTCAAGGTCGTCCACGGCGACGGCGATCCCACCGTTCGCCGCCAGGTTTTCAGAGAGATGGAGATTCTCCGCCGTACGGACTCTCCTTACGTCGTGAAATGCCATGGAATCTTCGAGAAGCCGTCTGGAGATGTCACGATTTTGATGGAGTATATGGATCGAGGATCGCTTGATACGCTCTTGAAGAAGAATACCACGCTGTCAGAGTCCACGCTCGCTCACGTATCGCGTCAGGTTCTTAATGGCCTTCACTACCTTCACTCTCATAAAATCATTCATCGCGATATTAAACCGTCGAATCTCTTGGTGAATAAGAATATGGAGGTTAAGATTGCCGATTTTGGAGTTAGTAAAATCATGTGTAGGACTCTGGATGCTTGCAATTCCTACGTCGGAACCTGCGCTTATATGAGTCCGGAGCGGTTCGATCCAGAGACTTACGGCGGAAATTACAACGGTTACGCCGGAGACATATGGAGTTTAGGCCTTACTCTTCTGGAACTCTATTTAGGCCATTTTCCTTTTCTTCCGCGTGGTCAGAGACCGGATTGGGCGACTCTGATGTTTGCGATTTGCTTTGATGAGCCGCCGACGCTGCCGGAGGATGCGTCAGAGGAGTTTCGAAGCTTCGTCCAGAGTTGTTTGCAGAAGGAATCGAGCAAGAGATGGACGGCGGCGCAATTATTAACGCATCCGTTTGTAAGTAAGGAATCGAGATCCGATCGGTGA

Protein sequence

MALVRDRLQLKLRLPDLSDCGPRFPLPLPPSSAPAISSADLEKLQVLGHGNGGTVYKVRHKRTSNIYALKVVHGDGDPTVRRQVFREMEILRRTDSPYVVKCHGIFEKPSGDVTILMEYMDRGSLDTLLKKNTTLSESTLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTLLELYLGHFPFLPRGQRPDWATLMFAICFDEPPTLPEDASEEFRSFVQSCLQKESSKRWTAAQLLTHPFVSKESRSDR
Homology
BLAST of Carg06640 vs. NCBI nr
Match: KAG7012161.1 (Mitogen-activated protein kinase kinase 9, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 639.0 bits (1647), Expect = 2.1e-179
Identity = 310/310 (100.00%), Postives = 310/310 (100.00%), Query Frame = 0

Query: 1   MALVRDRLQLKLRLPDLSDCGPRFPLPLPPSSAPAISSADLEKLQVLGHGNGGTVYKVRH 60
           MALVRDRLQLKLRLPDLSDCGPRFPLPLPPSSAPAISSADLEKLQVLGHGNGGTVYKVRH
Sbjct: 1   MALVRDRLQLKLRLPDLSDCGPRFPLPLPPSSAPAISSADLEKLQVLGHGNGGTVYKVRH 60

Query: 61  KRTSNIYALKVVHGDGDPTVRRQVFREMEILRRTDSPYVVKCHGIFEKPSGDVTILMEYM 120
           KRTSNIYALKVVHGDGDPTVRRQVFREMEILRRTDSPYVVKCHGIFEKPSGDVTILMEYM
Sbjct: 61  KRTSNIYALKVVHGDGDPTVRRQVFREMEILRRTDSPYVVKCHGIFEKPSGDVTILMEYM 120

Query: 121 DRGSLDTLLKKNTTLSESTLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNKNMEVKIA 180
           DRGSLDTLLKKNTTLSESTLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNKNMEVKIA
Sbjct: 121 DRGSLDTLLKKNTTLSESTLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNKNMEVKIA 180

Query: 181 DFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTLLELYLGH 240
           DFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTLLELYLGH
Sbjct: 181 DFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTLLELYLGH 240

Query: 241 FPFLPRGQRPDWATLMFAICFDEPPTLPEDASEEFRSFVQSCLQKESSKRWTAAQLLTHP 300
           FPFLPRGQRPDWATLMFAICFDEPPTLPEDASEEFRSFVQSCLQKESSKRWTAAQLLTHP
Sbjct: 241 FPFLPRGQRPDWATLMFAICFDEPPTLPEDASEEFRSFVQSCLQKESSKRWTAAQLLTHP 300

Query: 301 FVSKESRSDR 311
           FVSKESRSDR
Sbjct: 301 FVSKESRSDR 310

BLAST of Carg06640 vs. NCBI nr
Match: KAG6572979.1 (Mitogen-activated protein kinase kinase 9, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 636.7 bits (1641), Expect = 1.0e-178
Identity = 309/310 (99.68%), Postives = 309/310 (99.68%), Query Frame = 0

Query: 1   MALVRDRLQLKLRLPDLSDCGPRFPLPLPPSSAPAISSADLEKLQVLGHGNGGTVYKVRH 60
           MALVRDR QLKLRLPDLSDCGPRFPLPLPPSSAPAISSADLEKLQVLGHGNGGTVYKVRH
Sbjct: 1   MALVRDRRQLKLRLPDLSDCGPRFPLPLPPSSAPAISSADLEKLQVLGHGNGGTVYKVRH 60

Query: 61  KRTSNIYALKVVHGDGDPTVRRQVFREMEILRRTDSPYVVKCHGIFEKPSGDVTILMEYM 120
           KRTSNIYALKVVHGDGDPTVRRQVFREMEILRRTDSPYVVKCHGIFEKPSGDVTILMEYM
Sbjct: 61  KRTSNIYALKVVHGDGDPTVRRQVFREMEILRRTDSPYVVKCHGIFEKPSGDVTILMEYM 120

Query: 121 DRGSLDTLLKKNTTLSESTLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNKNMEVKIA 180
           DRGSLDTLLKKNTTLSESTLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNKNMEVKIA
Sbjct: 121 DRGSLDTLLKKNTTLSESTLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNKNMEVKIA 180

Query: 181 DFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTLLELYLGH 240
           DFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTLLELYLGH
Sbjct: 181 DFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTLLELYLGH 240

Query: 241 FPFLPRGQRPDWATLMFAICFDEPPTLPEDASEEFRSFVQSCLQKESSKRWTAAQLLTHP 300
           FPFLPRGQRPDWATLMFAICFDEPPTLPEDASEEFRSFVQSCLQKESSKRWTAAQLLTHP
Sbjct: 241 FPFLPRGQRPDWATLMFAICFDEPPTLPEDASEEFRSFVQSCLQKESSKRWTAAQLLTHP 300

Query: 301 FVSKESRSDR 311
           FVSKESRSDR
Sbjct: 301 FVSKESRSDR 310

BLAST of Carg06640 vs. NCBI nr
Match: XP_022955052.1 (mitogen-activated protein kinase kinase 9-like [Cucurbita moschata])

HSP 1 Score: 632.5 bits (1630), Expect = 1.9e-177
Identity = 307/310 (99.03%), Postives = 307/310 (99.03%), Query Frame = 0

Query: 1   MALVRDRLQLKLRLPDLSDCGPRFPLPLPPSSAPAISSADLEKLQVLGHGNGGTVYKVRH 60
           MALVRDR QLKLRLPDLSDCGPRFPLPLPPSSAPAISSADLEKLQVLGHGNGGTVYKVRH
Sbjct: 1   MALVRDRRQLKLRLPDLSDCGPRFPLPLPPSSAPAISSADLEKLQVLGHGNGGTVYKVRH 60

Query: 61  KRTSNIYALKVVHGDGDPTVRRQVFREMEILRRTDSPYVVKCHGIFEKPSGDVTILMEYM 120
           KRTSNIYA KVVHGDGDPTVRRQVFREMEILRRTDSPYVVKCHGI EKPSGDVTILMEYM
Sbjct: 61  KRTSNIYAFKVVHGDGDPTVRRQVFREMEILRRTDSPYVVKCHGIVEKPSGDVTILMEYM 120

Query: 121 DRGSLDTLLKKNTTLSESTLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNKNMEVKIA 180
           DRGSLDTLLKKNTTLSESTLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNKNMEVKIA
Sbjct: 121 DRGSLDTLLKKNTTLSESTLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNKNMEVKIA 180

Query: 181 DFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTLLELYLGH 240
           DFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTLLELYLGH
Sbjct: 181 DFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTLLELYLGH 240

Query: 241 FPFLPRGQRPDWATLMFAICFDEPPTLPEDASEEFRSFVQSCLQKESSKRWTAAQLLTHP 300
           FPFLPRGQRPDWATLMFAICFDEPPTLPEDASEEFRSFVQSCLQKESSKRWTAAQLLTHP
Sbjct: 241 FPFLPRGQRPDWATLMFAICFDEPPTLPEDASEEFRSFVQSCLQKESSKRWTAAQLLTHP 300

Query: 301 FVSKESRSDR 311
           FVSKESRSDR
Sbjct: 301 FVSKESRSDR 310

BLAST of Carg06640 vs. NCBI nr
Match: XP_023542980.1 (mitogen-activated protein kinase kinase 9-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 626.7 bits (1615), Expect = 1.1e-175
Identity = 304/310 (98.06%), Postives = 306/310 (98.71%), Query Frame = 0

Query: 1   MALVRDRLQLKLRLPDLSDCGPRFPLPLPPSSAPAISSADLEKLQVLGHGNGGTVYKVRH 60
           MALVRDR QLKLRLPDLSDCGPRFPLPLPPSSAPAISSADLEKLQVLGHGNGGTVYKVRH
Sbjct: 1   MALVRDRRQLKLRLPDLSDCGPRFPLPLPPSSAPAISSADLEKLQVLGHGNGGTVYKVRH 60

Query: 61  KRTSNIYALKVVHGDGDPTVRRQVFREMEILRRTDSPYVVKCHGIFEKPSGDVTILMEYM 120
           KRTSNIYALKVVHGDGDPTVRRQVFREMEILRRTDSPYVVKCHGIFEKPSGDVTILMEYM
Sbjct: 61  KRTSNIYALKVVHGDGDPTVRRQVFREMEILRRTDSPYVVKCHGIFEKPSGDVTILMEYM 120

Query: 121 DRGSLDTLLKKNTTLSESTLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNKNMEVKIA 180
           D GSLDTLLKKNTTLSE+TLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNKNMEVKIA
Sbjct: 121 DLGSLDTLLKKNTTLSEATLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNKNMEVKIA 180

Query: 181 DFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTLLELYLGH 240
           DFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTLLELYLGH
Sbjct: 181 DFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTLLELYLGH 240

Query: 241 FPFLPRGQRPDWATLMFAICFDEPPTLPEDASEEFRSFVQSCLQKESSKRWTAAQLLTHP 300
           FPFLPRGQRPDWATLMFAICF EPPTLPEDASEEFRSFVQSCLQKESSKRWTAAQLLTHP
Sbjct: 241 FPFLPRGQRPDWATLMFAICFGEPPTLPEDASEEFRSFVQSCLQKESSKRWTAAQLLTHP 300

Query: 301 FVSKESRSDR 311
           FV KESRSD+
Sbjct: 301 FVCKESRSDQ 310

BLAST of Carg06640 vs. NCBI nr
Match: XP_022994131.1 (mitogen-activated protein kinase kinase 9-like [Cucurbita maxima])

HSP 1 Score: 612.5 bits (1578), Expect = 2.1e-171
Identity = 297/306 (97.06%), Postives = 299/306 (97.71%), Query Frame = 0

Query: 1   MALVRDRLQLKLRLPDLSDCGPRFPLPLPPSSAPAISSADLEKLQVLGHGNGGTVYKVRH 60
           MA VRDR QL LRLPDLSDCGP FPLPLPPSSAPAISS+DLEKLQVLGHGNGGTVYKVRH
Sbjct: 1   MAQVRDRRQLILRLPDLSDCGPHFPLPLPPSSAPAISSSDLEKLQVLGHGNGGTVYKVRH 60

Query: 61  KRTSNIYALKVVHGDGDPTVRRQVFREMEILRRTDSPYVVKCHGIFEKPSGDVTILMEYM 120
           KRTSNIYALKVVHGDGDPTVRRQVFREMEILRRTDSPYVVKCHGIFEKPSGDVTILMEYM
Sbjct: 61  KRTSNIYALKVVHGDGDPTVRRQVFREMEILRRTDSPYVVKCHGIFEKPSGDVTILMEYM 120

Query: 121 DRGSLDTLLKKNTTLSESTLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNKNMEVKIA 180
           D GSLDTLLKKNTTLSE+TLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNKNMEVKIA
Sbjct: 121 DLGSLDTLLKKNTTLSEATLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNKNMEVKIA 180

Query: 181 DFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTLLELYLGH 240
           DFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTLLELYLGH
Sbjct: 181 DFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTLLELYLGH 240

Query: 241 FPFLPRGQRPDWATLMFAICFDEPPTLPEDASEEFRSFVQSCLQKESSKRWTAAQLLTHP 300
           FPFLPRGQRPDWATLMFAICF EPPTLPEDASEEFRSFVQSCLQKESSKRWTAAQLLTHP
Sbjct: 241 FPFLPRGQRPDWATLMFAICFGEPPTLPEDASEEFRSFVQSCLQKESSKRWTAAQLLTHP 300

Query: 301 FVSKES 307
           FV KES
Sbjct: 301 FVCKES 306

BLAST of Carg06640 vs. ExPASy Swiss-Prot
Match: Q9FX43 (Mitogen-activated protein kinase kinase 9 OS=Arabidopsis thaliana OX=3702 GN=MKK9 PE=1 SV=1)

HSP 1 Score: 396.0 bits (1016), Expect = 4.0e-109
Identity = 202/312 (64.74%), Postives = 237/312 (75.96%), Query Frame = 0

Query: 1   MALVRDRLQLKLRLPDLSDCGPRFPLPLPPSSAP------AISSADLEKLQVLGHGNGGT 60
           MALVR+R QL LRLP       RF      ++         IS+ DLEKL VLG GNGG 
Sbjct: 1   MALVRERRQLNLRLPLPPISDRRFSTSSSSATTTTVAGCNGISACDLEKLNVLGCGNGGI 60

Query: 61  VYKVRHKRTSNIYALKVVHGDGDPTVRRQVFREMEILRRTDSPYVVKCHGIFEKP-SGDV 120
           VYKVRHK TS IYALK V+GD DP   RQ+ REMEILRRTDSPYVVKCHGIFEKP  G+V
Sbjct: 61  VYKVRHKTTSEIYALKTVNGDMDPIFTRQLMREMEILRRTDSPYVVKCHGIFEKPVVGEV 120

Query: 121 TILMEYMDRGSLDTLLKKNTTLSESTLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK 180
           +ILMEYMD G+L++L      ++E  LA  ++Q+L GL YLH+ KI+HRDIKP+NLL+N 
Sbjct: 121 SILMEYMDGGTLESL---RGGVTEQKLAGFAKQILKGLSYLHALKIVHRDIKPANLLLNS 180

Query: 181 NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTL 240
             EVKIADFGVSKI+ R+LD+CNSYVGTCAYMSPERFD E+ GG+ + YAGDIWS GL +
Sbjct: 181 KNEVKIADFGVSKILVRSLDSCNSYVGTCAYMSPERFDSESSGGSSDIYAGDIWSFGLMM 240

Query: 241 LELYLGHFPFLPRGQRPDWATLMFAICFDEPPTLPEDASEEFRSFVQSCLQKESSKRWTA 300
           LEL +GHFP LP GQRPDWATLM A+CF EPP  PE  SEEFRSFV+ CL+K+SSKRWTA
Sbjct: 241 LELLVGHFPLLPPGQRPDWATLMCAVCFGEPPRAPEGCSEEFRSFVECCLRKDSSKRWTA 300

Query: 301 AQLLTHPFVSKE 306
            QLL HPF+ ++
Sbjct: 301 PQLLAHPFLRED 309

BLAST of Carg06640 vs. ExPASy Swiss-Prot
Match: Q9LPQ3 (Mitogen-activated protein kinase kinase 7 OS=Arabidopsis thaliana OX=3702 GN=MKK7 PE=1 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 1.2e-102
Identity = 197/309 (63.75%), Postives = 231/309 (74.76%), Query Frame = 0

Query: 1   MALVRDRLQLKLRL--PDLSDCGPRFPLPLPPSSAP----AISSADLEKLQVLGHGNGGT 60
           MALVR R Q+ LRL  P LS   P F      S+AP     IS++D+EKL VLG G+ G 
Sbjct: 1   MALVRKRRQINLRLPVPPLSVHLPWF--SFASSTAPVINNGISASDVEKLHVLGRGSSGI 60

Query: 61  VYKVRHKRTSNIYALKVVHGDGDPTVRRQVFREMEILRRTDSPYVVKCHGIFEKP-SGDV 120
           VYKV HK T  IYALK V+GD  P   RQ+ REMEILRRTDSPYVV+C GIFEKP  G+V
Sbjct: 61  VYKVHHKTTGEIYALKSVNGDMSPAFTRQLAREMEILRRTDSPYVVRCQGIFEKPIVGEV 120

Query: 121 TILMEYMDRGSLDTLLKKNTTLSESTLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK 180
           +ILMEYMD G+L++L      ++E  LA  SRQ+L GL YLHS KI+HRDIKP+NLL+N 
Sbjct: 121 SILMEYMDGGNLESL---RGAVTEKQLAGFSRQILKGLSYLHSLKIVHRDIKPANLLLNS 180

Query: 181 NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTL 240
             EVKIADFGVSKI+ R+LD CNSYVGTCAYMSPERFD    G N + YAGDIWS G+ +
Sbjct: 181 RNEVKIADFGVSKIITRSLDYCNSYVGTCAYMSPERFD-SAAGENSDVYAGDIWSFGVMI 240

Query: 241 LELYLGHFPFLPRGQRPDWATLMFAICFDEPPTLPEDASEEFRSFVQSCLQKESSKRWTA 300
           LEL++GHFP LP+GQRPDWATLM  +CF EPP  PE  S+EFRSFV  CL+KESS+RWTA
Sbjct: 241 LELFVGHFPLLPQGQRPDWATLMCVVCFGEPPRAPEGCSDEFRSFVDCCLRKESSERWTA 300

Query: 301 AQLLTHPFV 303
           +QLL HPF+
Sbjct: 301 SQLLGHPFL 303

BLAST of Carg06640 vs. ExPASy Swiss-Prot
Match: Q8RXG3 (Mitogen-activated protein kinase kinase 5 OS=Arabidopsis thaliana OX=3702 GN=MKK5 PE=1 SV=2)

HSP 1 Score: 315.1 bits (806), Expect = 9.0e-85
Identity = 167/317 (52.68%), Postives = 213/317 (67.19%), Query Frame = 0

Query: 4   VRDRLQLKLRLPDLSDCGPRFPLPLPP----SSAPAISSA------------DLEKLQVL 63
           +R R  L L LP   D     PLPLPP    SSAPA SSA            +LE++  +
Sbjct: 18  LRKRPDLSLPLPH-RDVALAVPLPLPPPSSSSSAPASSSAISTNISAAKSLSELERVNRI 77

Query: 64  GHGNGGTVYKVRHKRTSNIYALKVVHGDGDPTVRRQVFREMEILRRTDSPYVVKCHGIFE 123
           G G GGTVYKV H  TS  +ALKV++G+ + TVRRQ+ RE+EILR  D P VVKCH +F+
Sbjct: 78  GSGAGGTVYKVIHTPTSRPFALKVIYGNHEDTVRRQICREIEILRSVDHPNVVKCHDMFD 137

Query: 124 KPSGDVTILMEYMDRGSLDTLLKKNTTLSESTLAHVSRQVLNGLHYLHSHKIIHRDIKPS 183
             +G++ +L+E+MD+GSL+          E  LA +SRQ+L+GL YLH   I+HRDIKPS
Sbjct: 138 H-NGEIQVLLEFMDQGSLEGA----HIWQEQELADLSRQILSGLAYLHRRHIVHRDIKPS 197

Query: 184 NLLVNKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIW 243
           NLL+N    VKIADFGVS+I+ +T+D CNS VGT AYMSPER + +   G Y+GYAGD+W
Sbjct: 198 NLLINSAKNVKIADFGVSRILAQTMDPCNSSVGTIAYMSPERINTDLNHGRYDGYAGDVW 257

Query: 244 SLGLTLLELYLGHFPFLPRGQRPDWATLMFAICFDEPPTLPEDASEEFRSFVQSCLQKES 303
           SLG+++LE YLG FPF    ++ DWA+LM AIC  +PP  P  AS+EFR FV  CLQ + 
Sbjct: 258 SLGVSILEFYLGRFPFAV-SRQGDWASLMCAICMSQPPEAPATASQEFRHFVSCCLQSDP 317

Query: 304 SKRWTAAQLLTHPFVSK 305
            KRW+A QLL HPF+ K
Sbjct: 318 PKRWSAQQLLQHPFILK 327

BLAST of Carg06640 vs. ExPASy Swiss-Prot
Match: O80397 (Mitogen-activated protein kinase kinase 4 OS=Arabidopsis thaliana OX=3702 GN=MKK4 PE=1 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 1.2e-81
Identity = 164/329 (49.85%), Postives = 213/329 (64.74%), Query Frame = 0

Query: 5   RDRLQLKLRLPDLSDCGPRFPLPLPP-------------------------SSAPAISSA 64
           R R  L L LP   D     PLPLPP                         SS  A + +
Sbjct: 19  RRRPDLTLPLPQ-RDVSLAVPLPLPPTSGGSGGSSGSAPSSGGSASSTNTNSSIEAKNYS 78

Query: 65  DLEKLQVLGHGNGGTVYKVRHKRTSNIYALKVVHGDGDPTVRRQVFREMEILRRTDSPYV 124
           DL +   +G G GGTVYKV H+ +S +YALKV++G+ + TVRRQ+ RE+EILR  + P V
Sbjct: 79  DLVRGNRIGSGAGGTVYKVIHRPSSRLYALKVIYGNHEETVRRQICREIEILRDVNHPNV 138

Query: 125 VKCHGIFEKPSGDVTILMEYMDRGSLDTLLKKNTTLSESTLAHVSRQVLNGLHYLHSHKI 184
           VKCH +F++ +G++ +L+E+MD+GSL+          E  LA +SRQ+L+GL YLHS  I
Sbjct: 139 VKCHEMFDQ-NGEIQVLLEFMDKGSLEGA----HVWKEQQLADLSRQILSGLAYLHSRHI 198

Query: 185 IHRDIKPSNLLVNKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNY 244
           +HRDIKPSNLL+N    VKIADFGVS+I+ +T+D CNS VGT AYMSPER + +   G Y
Sbjct: 199 VHRDIKPSNLLINSAKNVKIADFGVSRILAQTMDPCNSSVGTIAYMSPERINTDLNQGKY 258

Query: 245 NGYAGDIWSLGLTLLELYLGHFPFLPRGQRPDWATLMFAICFDEPPTLPEDASEEFRSFV 304
           +GYAGDIWSLG+++LE YLG FPF P  ++ DWA+LM AIC  +PP  P  AS EFR F+
Sbjct: 259 DGYAGDIWSLGVSILEFYLGRFPF-PVSRQGDWASLMCAICMSQPPEAPATASPEFRHFI 318

Query: 305 QSCLQKESSKRWTAAQLLTHPFVSKESRS 309
             CLQ+E  KR +A QLL HPF+ + S S
Sbjct: 319 SCCLQREPGKRRSAMQLLQHPFILRASPS 340

BLAST of Carg06640 vs. ExPASy Swiss-Prot
Match: Q9M8J5 (Mitogen-activated protein kinase kinase 8 OS=Arabidopsis thaliana OX=3702 GN=MKK8 PE=3 SV=2)

HSP 1 Score: 303.5 bits (776), Expect = 2.7e-81
Identity = 164/323 (50.77%), Postives = 214/323 (66.25%), Query Frame = 0

Query: 1   MALVRDRLQLKLRLPDLSDCGPRFPLPLPPSSAPAI-----------------SSADLEK 60
           M +VRD   L L+L  +     + P  +PP   P I                 S A+L++
Sbjct: 1   MVMVRDNQFLNLKLSPI-----QAPTTIPPCRFPIIPATKVSATVSSCASNTFSVANLDR 60

Query: 61  LQVLGHGNGGTVYKVRHKRTSNIYALKVVHGDGDPTVRRQVFREMEILRRTDSPYVVKCH 120
           + VLG GNGGTV+KV+ K TS IYALK V  + D T      RE+EILR  +SPYV KCH
Sbjct: 61  ISVLGSGNGGTVFKVKDKTTSEIYALKKVKENWDST----SLREIEILRMVNSPYVAKCH 120

Query: 121 GIFEKPSGDVTILMEYMDRGSLDTLLKKNTTLSESTLAHVSRQVLNGLHYLHSHKIIHRD 180
            IF+ PSG+V+ILM+YMD GSL++L      ++E  LA +SRQVL G +YLH HKI+HRD
Sbjct: 121 DIFQNPSGEVSILMDYMDLGSLESL----RGVTEKQLALMSRQVLEGKNYLHEHKIVHRD 180

Query: 181 IKPSNLLVNKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYG----GNY 240
           IKP+NLL +   EVKIADFGVSKI+ R+L+ CNS+VGT AYMSPER D E  G       
Sbjct: 181 IKPANLLRSSKEEVKIADFGVSKIVVRSLNKCNSFVGTFAYMSPERLDSEADGVTEEDKS 240

Query: 241 NGYAGDIWSLGLTLLELYLGHFPFLPRGQRPDWATLMFAICFDEPPTLPEDASEEFRSFV 300
           N YAGDIWS GLT+LE+ +G++P L     PD A ++ A+CF EPP  PE+ S++ +SF+
Sbjct: 241 NVYAGDIWSFGLTMLEILVGYYPML-----PDQAAIVCAVCFGEPPKAPEECSDDLKSFM 300

Query: 301 QSCLQKESSKRWTAAQLLTHPFV 303
             CL+K++S+RWTA+QLL HPF+
Sbjct: 301 DCCLRKKASERWTASQLLNHPFL 305

BLAST of Carg06640 vs. ExPASy TrEMBL
Match: A0A6J1GSI8 (mitogen-activated protein kinase kinase 9-like OS=Cucurbita moschata OX=3662 GN=LOC111457129 PE=3 SV=1)

HSP 1 Score: 632.5 bits (1630), Expect = 9.4e-178
Identity = 307/310 (99.03%), Postives = 307/310 (99.03%), Query Frame = 0

Query: 1   MALVRDRLQLKLRLPDLSDCGPRFPLPLPPSSAPAISSADLEKLQVLGHGNGGTVYKVRH 60
           MALVRDR QLKLRLPDLSDCGPRFPLPLPPSSAPAISSADLEKLQVLGHGNGGTVYKVRH
Sbjct: 1   MALVRDRRQLKLRLPDLSDCGPRFPLPLPPSSAPAISSADLEKLQVLGHGNGGTVYKVRH 60

Query: 61  KRTSNIYALKVVHGDGDPTVRRQVFREMEILRRTDSPYVVKCHGIFEKPSGDVTILMEYM 120
           KRTSNIYA KVVHGDGDPTVRRQVFREMEILRRTDSPYVVKCHGI EKPSGDVTILMEYM
Sbjct: 61  KRTSNIYAFKVVHGDGDPTVRRQVFREMEILRRTDSPYVVKCHGIVEKPSGDVTILMEYM 120

Query: 121 DRGSLDTLLKKNTTLSESTLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNKNMEVKIA 180
           DRGSLDTLLKKNTTLSESTLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNKNMEVKIA
Sbjct: 121 DRGSLDTLLKKNTTLSESTLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNKNMEVKIA 180

Query: 181 DFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTLLELYLGH 240
           DFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTLLELYLGH
Sbjct: 181 DFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTLLELYLGH 240

Query: 241 FPFLPRGQRPDWATLMFAICFDEPPTLPEDASEEFRSFVQSCLQKESSKRWTAAQLLTHP 300
           FPFLPRGQRPDWATLMFAICFDEPPTLPEDASEEFRSFVQSCLQKESSKRWTAAQLLTHP
Sbjct: 241 FPFLPRGQRPDWATLMFAICFDEPPTLPEDASEEFRSFVQSCLQKESSKRWTAAQLLTHP 300

Query: 301 FVSKESRSDR 311
           FVSKESRSDR
Sbjct: 301 FVSKESRSDR 310

BLAST of Carg06640 vs. ExPASy TrEMBL
Match: A0A6J1JY92 (mitogen-activated protein kinase kinase 9-like OS=Cucurbita maxima OX=3661 GN=LOC111489959 PE=3 SV=1)

HSP 1 Score: 612.5 bits (1578), Expect = 1.0e-171
Identity = 297/306 (97.06%), Postives = 299/306 (97.71%), Query Frame = 0

Query: 1   MALVRDRLQLKLRLPDLSDCGPRFPLPLPPSSAPAISSADLEKLQVLGHGNGGTVYKVRH 60
           MA VRDR QL LRLPDLSDCGP FPLPLPPSSAPAISS+DLEKLQVLGHGNGGTVYKVRH
Sbjct: 1   MAQVRDRRQLILRLPDLSDCGPHFPLPLPPSSAPAISSSDLEKLQVLGHGNGGTVYKVRH 60

Query: 61  KRTSNIYALKVVHGDGDPTVRRQVFREMEILRRTDSPYVVKCHGIFEKPSGDVTILMEYM 120
           KRTSNIYALKVVHGDGDPTVRRQVFREMEILRRTDSPYVVKCHGIFEKPSGDVTILMEYM
Sbjct: 61  KRTSNIYALKVVHGDGDPTVRRQVFREMEILRRTDSPYVVKCHGIFEKPSGDVTILMEYM 120

Query: 121 DRGSLDTLLKKNTTLSESTLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNKNMEVKIA 180
           D GSLDTLLKKNTTLSE+TLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNKNMEVKIA
Sbjct: 121 DLGSLDTLLKKNTTLSEATLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNKNMEVKIA 180

Query: 181 DFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTLLELYLGH 240
           DFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTLLELYLGH
Sbjct: 181 DFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTLLELYLGH 240

Query: 241 FPFLPRGQRPDWATLMFAICFDEPPTLPEDASEEFRSFVQSCLQKESSKRWTAAQLLTHP 300
           FPFLPRGQRPDWATLMFAICF EPPTLPEDASEEFRSFVQSCLQKESSKRWTAAQLLTHP
Sbjct: 241 FPFLPRGQRPDWATLMFAICFGEPPTLPEDASEEFRSFVQSCLQKESSKRWTAAQLLTHP 300

Query: 301 FVSKES 307
           FV KES
Sbjct: 301 FVCKES 306

BLAST of Carg06640 vs. ExPASy TrEMBL
Match: A0A5D3BGX9 (Mitogen-activated protein kinase kinase 9 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002540 PE=3 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 1.9e-162
Identity = 285/313 (91.05%), Postives = 292/313 (93.29%), Query Frame = 0

Query: 1   MALVRDRLQLKLRLPDLSDCGPRFPLPLPPSSAP-------AISSADLEKLQVLGHGNGG 60
           MALVRDR  L LRLPDLSDC PRFPLPLPPSSAP       AISS+DLEKLQVLGHGNGG
Sbjct: 1   MALVRDRRHLNLRLPDLSDCRPRFPLPLPPSSAPPAPAAPSAISSSDLEKLQVLGHGNGG 60

Query: 61  TVYKVRHKRTSNIYALKVVHGDGDPTVRRQVFREMEILRRTDSPYVVKCHGIFEKPSGDV 120
           TVYKVRHKRTS  YALKVVHGD DPTVRRQVFREMEILRRTDSPYVV+CHGIFEKPSGDV
Sbjct: 61  TVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSGDV 120

Query: 121 TILMEYMDRGSLDTLLKKNTTLSESTLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK 180
           TILMEYMD GSLD+LLKKN+TLSE+TLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK
Sbjct: 121 TILMEYMDLGSLDSLLKKNSTLSETTLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK 180

Query: 181 NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTL 240
           NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTL
Sbjct: 181 NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTL 240

Query: 241 LELYLGHFPFLPRGQRPDWATLMFAICFDEPPTLPEDASEEFRSFVQSCLQKESSKRWTA 300
           LELYLGHFPFLP GQRPDWATLM AICF EPP LPEDASEEFRSFV+ CLQKESSKRWTA
Sbjct: 241 LELYLGHFPFLPAGQRPDWATLMCAICFGEPPKLPEDASEEFRSFVECCLQKESSKRWTA 300

Query: 301 AQLLTHPFVSKES 307
           AQLLTHPFV +ES
Sbjct: 301 AQLLTHPFVCRES 313

BLAST of Carg06640 vs. ExPASy TrEMBL
Match: A0A1S3CS75 (mitogen-activated protein kinase kinase 9 OS=Cucumis melo OX=3656 GN=LOC103504136 PE=3 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 1.9e-162
Identity = 285/313 (91.05%), Postives = 292/313 (93.29%), Query Frame = 0

Query: 1   MALVRDRLQLKLRLPDLSDCGPRFPLPLPPSSAP-------AISSADLEKLQVLGHGNGG 60
           MALVRDR  L LRLPDLSDC PRFPLPLPPSSAP       AISS+DLEKLQVLGHGNGG
Sbjct: 1   MALVRDRRHLNLRLPDLSDCRPRFPLPLPPSSAPPAPAAPSAISSSDLEKLQVLGHGNGG 60

Query: 61  TVYKVRHKRTSNIYALKVVHGDGDPTVRRQVFREMEILRRTDSPYVVKCHGIFEKPSGDV 120
           TVYKVRHKRTS  YALKVVHGD DPTVRRQVFREMEILRRTDSPYVV+CHGIFEKPSGDV
Sbjct: 61  TVYKVRHKRTSTTYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSGDV 120

Query: 121 TILMEYMDRGSLDTLLKKNTTLSESTLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK 180
           TILMEYMD GSLD+LLKKN+TLSE+TLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK
Sbjct: 121 TILMEYMDLGSLDSLLKKNSTLSETTLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK 180

Query: 181 NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTL 240
           NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTL
Sbjct: 181 NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTL 240

Query: 241 LELYLGHFPFLPRGQRPDWATLMFAICFDEPPTLPEDASEEFRSFVQSCLQKESSKRWTA 300
           LELYLGHFPFLP GQRPDWATLM AICF EPP LPEDASEEFRSFV+ CLQKESSKRWTA
Sbjct: 241 LELYLGHFPFLPAGQRPDWATLMCAICFGEPPKLPEDASEEFRSFVECCLQKESSKRWTA 300

Query: 301 AQLLTHPFVSKES 307
           AQLLTHPFV +ES
Sbjct: 301 AQLLTHPFVCRES 313

BLAST of Carg06640 vs. ExPASy TrEMBL
Match: A0A6J1KRM1 (mitogen-activated protein kinase kinase 9-like OS=Cucurbita maxima OX=3661 GN=LOC111495833 PE=3 SV=1)

HSP 1 Score: 581.3 bits (1497), Expect = 2.5e-162
Identity = 287/319 (89.97%), Postives = 296/319 (92.79%), Query Frame = 0

Query: 1   MALVRDRLQLKLRLPDLSDCGPRFPLPLPPSS--APAISSA-------DLEKLQVLGHGN 60
           MALVRDR QL LRLPDLSDC PRFPLPLPPSS  APA ++A       DLEKLQVLGHGN
Sbjct: 1   MALVRDRRQLNLRLPDLSDCRPRFPLPLPPSSAAAPAATAAAAAPAASDLEKLQVLGHGN 60

Query: 61  GGTVYKVRHKRTSNIYALKVVHGDGDPTVRRQVFREMEILRRTDSPYVVKCHGIFEKPSG 120
           GGTVYKVRHKRTS  YALKVVHGD DPTVRRQVFREMEILRRTDSPYVV+CHGIFEKPSG
Sbjct: 61  GGTVYKVRHKRTSATYALKVVHGDCDPTVRRQVFREMEILRRTDSPYVVQCHGIFEKPSG 120

Query: 121 DVTILMEYMDRGSLDTLLKKNTTLSESTLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLV 180
           DVTILMEYMD GSLD+LLKKN+TLSESTLAHV+RQVLNGLHYLHSHKIIHRDIKPSNLLV
Sbjct: 121 DVTILMEYMDLGSLDSLLKKNSTLSESTLAHVARQVLNGLHYLHSHKIIHRDIKPSNLLV 180

Query: 181 NKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGL 240
           NKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDP+TYGGNYNGYAGDIWSLGL
Sbjct: 181 NKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPDTYGGNYNGYAGDIWSLGL 240

Query: 241 TLLELYLGHFPFLPRGQRPDWATLMFAICFDEPPTLPEDASEEFRSFVQSCLQKESSKRW 300
           TLLELYLGHFPFLP GQRPDWATLM AICF EPPTLPEDASE+FRSFV+ CLQKESSKRW
Sbjct: 241 TLLELYLGHFPFLPPGQRPDWATLMCAICFGEPPTLPEDASEDFRSFVECCLQKESSKRW 300

Query: 301 TAAQLLTHPFVSKESRSDR 311
           T AQLLTHPFV KESRSDR
Sbjct: 301 TVAQLLTHPFVCKESRSDR 319

BLAST of Carg06640 vs. TAIR 10
Match: AT1G73500.1 (MAP kinase kinase 9 )

HSP 1 Score: 396.0 bits (1016), Expect = 2.8e-110
Identity = 202/312 (64.74%), Postives = 237/312 (75.96%), Query Frame = 0

Query: 1   MALVRDRLQLKLRLPDLSDCGPRFPLPLPPSSAP------AISSADLEKLQVLGHGNGGT 60
           MALVR+R QL LRLP       RF      ++         IS+ DLEKL VLG GNGG 
Sbjct: 1   MALVRERRQLNLRLPLPPISDRRFSTSSSSATTTTVAGCNGISACDLEKLNVLGCGNGGI 60

Query: 61  VYKVRHKRTSNIYALKVVHGDGDPTVRRQVFREMEILRRTDSPYVVKCHGIFEKP-SGDV 120
           VYKVRHK TS IYALK V+GD DP   RQ+ REMEILRRTDSPYVVKCHGIFEKP  G+V
Sbjct: 61  VYKVRHKTTSEIYALKTVNGDMDPIFTRQLMREMEILRRTDSPYVVKCHGIFEKPVVGEV 120

Query: 121 TILMEYMDRGSLDTLLKKNTTLSESTLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK 180
           +ILMEYMD G+L++L      ++E  LA  ++Q+L GL YLH+ KI+HRDIKP+NLL+N 
Sbjct: 121 SILMEYMDGGTLESL---RGGVTEQKLAGFAKQILKGLSYLHALKIVHRDIKPANLLLNS 180

Query: 181 NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTL 240
             EVKIADFGVSKI+ R+LD+CNSYVGTCAYMSPERFD E+ GG+ + YAGDIWS GL +
Sbjct: 181 KNEVKIADFGVSKILVRSLDSCNSYVGTCAYMSPERFDSESSGGSSDIYAGDIWSFGLMM 240

Query: 241 LELYLGHFPFLPRGQRPDWATLMFAICFDEPPTLPEDASEEFRSFVQSCLQKESSKRWTA 300
           LEL +GHFP LP GQRPDWATLM A+CF EPP  PE  SEEFRSFV+ CL+K+SSKRWTA
Sbjct: 241 LELLVGHFPLLPPGQRPDWATLMCAVCFGEPPRAPEGCSEEFRSFVECCLRKDSSKRWTA 300

Query: 301 AQLLTHPFVSKE 306
            QLL HPF+ ++
Sbjct: 301 PQLLAHPFLRED 309

BLAST of Carg06640 vs. TAIR 10
Match: AT1G18350.1 (MAP kinase kinase 7 )

HSP 1 Score: 374.4 bits (960), Expect = 8.8e-104
Identity = 197/309 (63.75%), Postives = 231/309 (74.76%), Query Frame = 0

Query: 1   MALVRDRLQLKLRL--PDLSDCGPRFPLPLPPSSAP----AISSADLEKLQVLGHGNGGT 60
           MALVR R Q+ LRL  P LS   P F      S+AP     IS++D+EKL VLG G+ G 
Sbjct: 1   MALVRKRRQINLRLPVPPLSVHLPWF--SFASSTAPVINNGISASDVEKLHVLGRGSSGI 60

Query: 61  VYKVRHKRTSNIYALKVVHGDGDPTVRRQVFREMEILRRTDSPYVVKCHGIFEKP-SGDV 120
           VYKV HK T  IYALK V+GD  P   RQ+ REMEILRRTDSPYVV+C GIFEKP  G+V
Sbjct: 61  VYKVHHKTTGEIYALKSVNGDMSPAFTRQLAREMEILRRTDSPYVVRCQGIFEKPIVGEV 120

Query: 121 TILMEYMDRGSLDTLLKKNTTLSESTLAHVSRQVLNGLHYLHSHKIIHRDIKPSNLLVNK 180
           +ILMEYMD G+L++L      ++E  LA  SRQ+L GL YLHS KI+HRDIKP+NLL+N 
Sbjct: 121 SILMEYMDGGNLESL---RGAVTEKQLAGFSRQILKGLSYLHSLKIVHRDIKPANLLLNS 180

Query: 181 NMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIWSLGLTL 240
             EVKIADFGVSKI+ R+LD CNSYVGTCAYMSPERFD    G N + YAGDIWS G+ +
Sbjct: 181 RNEVKIADFGVSKIITRSLDYCNSYVGTCAYMSPERFD-SAAGENSDVYAGDIWSFGVMI 240

Query: 241 LELYLGHFPFLPRGQRPDWATLMFAICFDEPPTLPEDASEEFRSFVQSCLQKESSKRWTA 300
           LEL++GHFP LP+GQRPDWATLM  +CF EPP  PE  S+EFRSFV  CL+KESS+RWTA
Sbjct: 241 LELFVGHFPLLPQGQRPDWATLMCVVCFGEPPRAPEGCSDEFRSFVDCCLRKESSERWTA 300

Query: 301 AQLLTHPFV 303
           +QLL HPF+
Sbjct: 301 SQLLGHPFL 303

BLAST of Carg06640 vs. TAIR 10
Match: AT3G21220.1 (MAP kinase kinase 5 )

HSP 1 Score: 315.1 bits (806), Expect = 6.4e-86
Identity = 167/317 (52.68%), Postives = 213/317 (67.19%), Query Frame = 0

Query: 4   VRDRLQLKLRLPDLSDCGPRFPLPLPP----SSAPAISSA------------DLEKLQVL 63
           +R R  L L LP   D     PLPLPP    SSAPA SSA            +LE++  +
Sbjct: 18  LRKRPDLSLPLPH-RDVALAVPLPLPPPSSSSSAPASSSAISTNISAAKSLSELERVNRI 77

Query: 64  GHGNGGTVYKVRHKRTSNIYALKVVHGDGDPTVRRQVFREMEILRRTDSPYVVKCHGIFE 123
           G G GGTVYKV H  TS  +ALKV++G+ + TVRRQ+ RE+EILR  D P VVKCH +F+
Sbjct: 78  GSGAGGTVYKVIHTPTSRPFALKVIYGNHEDTVRRQICREIEILRSVDHPNVVKCHDMFD 137

Query: 124 KPSGDVTILMEYMDRGSLDTLLKKNTTLSESTLAHVSRQVLNGLHYLHSHKIIHRDIKPS 183
             +G++ +L+E+MD+GSL+          E  LA +SRQ+L+GL YLH   I+HRDIKPS
Sbjct: 138 H-NGEIQVLLEFMDQGSLEGA----HIWQEQELADLSRQILSGLAYLHRRHIVHRDIKPS 197

Query: 184 NLLVNKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNYNGYAGDIW 243
           NLL+N    VKIADFGVS+I+ +T+D CNS VGT AYMSPER + +   G Y+GYAGD+W
Sbjct: 198 NLLINSAKNVKIADFGVSRILAQTMDPCNSSVGTIAYMSPERINTDLNHGRYDGYAGDVW 257

Query: 244 SLGLTLLELYLGHFPFLPRGQRPDWATLMFAICFDEPPTLPEDASEEFRSFVQSCLQKES 303
           SLG+++LE YLG FPF    ++ DWA+LM AIC  +PP  P  AS+EFR FV  CLQ + 
Sbjct: 258 SLGVSILEFYLGRFPFAV-SRQGDWASLMCAICMSQPPEAPATASQEFRHFVSCCLQSDP 317

Query: 304 SKRWTAAQLLTHPFVSK 305
            KRW+A QLL HPF+ K
Sbjct: 318 PKRWSAQQLLQHPFILK 327

BLAST of Carg06640 vs. TAIR 10
Match: AT1G51660.1 (mitogen-activated protein kinase kinase 4 )

HSP 1 Score: 304.7 bits (779), Expect = 8.6e-83
Identity = 164/329 (49.85%), Postives = 213/329 (64.74%), Query Frame = 0

Query: 5   RDRLQLKLRLPDLSDCGPRFPLPLPP-------------------------SSAPAISSA 64
           R R  L L LP   D     PLPLPP                         SS  A + +
Sbjct: 19  RRRPDLTLPLPQ-RDVSLAVPLPLPPTSGGSGGSSGSAPSSGGSASSTNTNSSIEAKNYS 78

Query: 65  DLEKLQVLGHGNGGTVYKVRHKRTSNIYALKVVHGDGDPTVRRQVFREMEILRRTDSPYV 124
           DL +   +G G GGTVYKV H+ +S +YALKV++G+ + TVRRQ+ RE+EILR  + P V
Sbjct: 79  DLVRGNRIGSGAGGTVYKVIHRPSSRLYALKVIYGNHEETVRRQICREIEILRDVNHPNV 138

Query: 125 VKCHGIFEKPSGDVTILMEYMDRGSLDTLLKKNTTLSESTLAHVSRQVLNGLHYLHSHKI 184
           VKCH +F++ +G++ +L+E+MD+GSL+          E  LA +SRQ+L+GL YLHS  I
Sbjct: 139 VKCHEMFDQ-NGEIQVLLEFMDKGSLEGA----HVWKEQQLADLSRQILSGLAYLHSRHI 198

Query: 185 IHRDIKPSNLLVNKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYGGNY 244
           +HRDIKPSNLL+N    VKIADFGVS+I+ +T+D CNS VGT AYMSPER + +   G Y
Sbjct: 199 VHRDIKPSNLLINSAKNVKIADFGVSRILAQTMDPCNSSVGTIAYMSPERINTDLNQGKY 258

Query: 245 NGYAGDIWSLGLTLLELYLGHFPFLPRGQRPDWATLMFAICFDEPPTLPEDASEEFRSFV 304
           +GYAGDIWSLG+++LE YLG FPF P  ++ DWA+LM AIC  +PP  P  AS EFR F+
Sbjct: 259 DGYAGDIWSLGVSILEFYLGRFPF-PVSRQGDWASLMCAICMSQPPEAPATASPEFRHFI 318

Query: 305 QSCLQKESSKRWTAAQLLTHPFVSKESRS 309
             CLQ+E  KR +A QLL HPF+ + S S
Sbjct: 319 SCCLQREPGKRRSAMQLLQHPFILRASPS 340

BLAST of Carg06640 vs. TAIR 10
Match: AT3G06230.1 (MAP kinase kinase 8 )

HSP 1 Score: 281.2 bits (718), Expect = 1.0e-75
Identity = 155/311 (49.84%), Postives = 203/311 (65.27%), Query Frame = 0

Query: 1   MALVRDRLQLKLRLPDLSDCGPRFPLPLPPSSAPAI-----------------SSADLEK 60
           M +VRD   L L+L  +     + P  +PP   P I                 S A+L++
Sbjct: 1   MVMVRDNQFLNLKLSPI-----QAPTTIPPCRFPIIPATKVSATVSSCASNTFSVANLDR 60

Query: 61  LQVLGHGNGGTVYKVRHKRTSNIYALKVVHGDGDPTVRRQVFREMEILRRTDSPYVVKCH 120
           + VLG GNGGTV+KV+ K TS IYALK V  + D T      RE+EILR  +SPYV KCH
Sbjct: 61  ISVLGSGNGGTVFKVKDKTTSEIYALKKVKENWDST----SLREIEILRMVNSPYVAKCH 120

Query: 121 GIFEKPSGDVTILMEYMDRGSLDTLLKKNTTLSESTLAHVSRQVLNGLHYLHSHKIIHRD 180
            IF+ PSG+V+ILM+YMD GSL++L      ++E  LA +SRQVL G +YLH HKI+HRD
Sbjct: 121 DIFQNPSGEVSILMDYMDLGSLESL----RGVTEKQLALMSRQVLEGKNYLHEHKIVHRD 180

Query: 181 IKPSNLLVNKNMEVKIADFGVSKIMCRTLDACNSYVGTCAYMSPERFDPETYG----GNY 240
           IKP+NLL +   EVKIADFGVSKI+ R+L+ CNS+VGT AYMSPER D E  G       
Sbjct: 181 IKPANLLRSSKEEVKIADFGVSKIVVRSLNKCNSFVGTFAYMSPERLDSEADGVTEEDKS 240

Query: 241 NGYAGDIWSLGLTLLELYLGHFPFLPRGQRPDWATLMFAICFDEPPTLPEDASEEFRSFV 291
           N YAGDIWS GLT+LE+ +G++P L     PD A ++ A+CF EPP  PE+ S++ +SF+
Sbjct: 241 NVYAGDIWSFGLTMLEILVGYYPML-----PDQAAIVCAVCFGEPPKAPEECSDDLKSFM 293

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7012161.12.1e-179100.00Mitogen-activated protein kinase kinase 9, partial [Cucurbita argyrosperma subsp... [more]
KAG6572979.11.0e-17899.68Mitogen-activated protein kinase kinase 9, partial [Cucurbita argyrosperma subsp... [more]
XP_022955052.11.9e-17799.03mitogen-activated protein kinase kinase 9-like [Cucurbita moschata][more]
XP_023542980.11.1e-17598.06mitogen-activated protein kinase kinase 9-like [Cucurbita pepo subsp. pepo][more]
XP_022994131.12.1e-17197.06mitogen-activated protein kinase kinase 9-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9FX434.0e-10964.74Mitogen-activated protein kinase kinase 9 OS=Arabidopsis thaliana OX=3702 GN=MKK... [more]
Q9LPQ31.2e-10263.75Mitogen-activated protein kinase kinase 7 OS=Arabidopsis thaliana OX=3702 GN=MKK... [more]
Q8RXG39.0e-8552.68Mitogen-activated protein kinase kinase 5 OS=Arabidopsis thaliana OX=3702 GN=MKK... [more]
O803971.2e-8149.85Mitogen-activated protein kinase kinase 4 OS=Arabidopsis thaliana OX=3702 GN=MKK... [more]
Q9M8J52.7e-8150.77Mitogen-activated protein kinase kinase 8 OS=Arabidopsis thaliana OX=3702 GN=MKK... [more]
Match NameE-valueIdentityDescription
A0A6J1GSI89.4e-17899.03mitogen-activated protein kinase kinase 9-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1JY921.0e-17197.06mitogen-activated protein kinase kinase 9-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A5D3BGX91.9e-16291.05Mitogen-activated protein kinase kinase 9 OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3CS751.9e-16291.05mitogen-activated protein kinase kinase 9 OS=Cucumis melo OX=3656 GN=LOC10350413... [more]
A0A6J1KRM12.5e-16289.97mitogen-activated protein kinase kinase 9-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
Match NameE-valueIdentityDescription
AT1G73500.12.8e-11064.74MAP kinase kinase 9 [more]
AT1G18350.18.8e-10463.75MAP kinase kinase 7 [more]
AT3G21220.16.4e-8652.68MAP kinase kinase 5 [more]
AT1G51660.18.6e-8349.85mitogen-activated protein kinase kinase 4 [more]
AT3G06230.11.0e-7549.84MAP kinase kinase 8 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPRINTSPR00109TYRKINASEcoord: 117..130
score: 43.81
coord: 224..246
score: 27.95
coord: 153..171
score: 45.74
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 41..302
e-value: 1.4E-92
score: 323.6
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 42..302
e-value: 1.1E-67
score: 228.2
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 41..302
score: 47.028778
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 123..310
e-value: 1.4E-56
score: 193.8
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 30..121
e-value: 4.7E-30
score: 105.8
NoneNo IPR availablePIRSRPIRSR000620-1PIRSR000620-1coord: 42..297
e-value: 3.8E-17
score: 60.1
NoneNo IPR availablePANTHERPTHR24361:SF769SERINE/THREONINE-PROTEIN KINASE C01C4.3-RELATEDcoord: 5..307
NoneNo IPR availablePANTHERPTHR24361MITOGEN-ACTIVATED KINASE KINASE KINASEcoord: 5..307
NoneNo IPR availableCDDcd06623PKc_MAPKK_plant_likecoord: 39..304
e-value: 9.66476E-143
score: 401.2
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 47..70
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 159..171
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 41..303

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg06640-RACarg06640-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0018105 peptidyl-serine phosphorylation
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity