Carg06534 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg06534
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	PPPDE domain-containing protein
Location	Carg_Chr14: 10559543 .. 10561623 (-)
RNA-Seq Expression	Carg06534
Synteny	Carg06534

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CAATGGAGTTGTTATTAATCGCAGTAAGCGAGTGGGAGTGAGATCGAAATGTGGTGCAGAAGAATCTCCTGCAATGGCGACTCTGGATCGGTGCCAGTGTACCTCAATGTGTACGACCTGACGCACATTAATGGCTACGCATATTGGCTTGGCCTCGGAGTTTATCATTCTGGTGTACAAGGTTCGTTCTAATTCGAGCTTTTCAATTCTTCTTCGTCTAATTCATCGGTTTATTTGCTGGAATTTGCTGTTAGATCTGCATTTTTTATGCTTAATCTGCTGGAATCCACTTTTGATGAGTTCTGTTTCTGTTTGGTTAGTGAGAAAATGGGGGGAAATTGATTGAACTTCTAGGTTTTTGCTTTCTGTATACTTGTTTATTGTTCTTGTAAGTCGGATTTTACTCCTGTGAAAACTTTCCCGATGTTTTCTGGACCTGCATTTTGATGGATTCTGTTTGGATGGTGAGAAAATGATGGAAAATTAGACGAACTTCTAGGTTTTTACATTTAGTACTCTTGTTTGTTGTCTTCAAGTATAATTTGCTCTGTTTGTGACCTCCATTTTGACGGAATCTGTTTGGATGGTGAGAAAATGGAAGAAAATTGGACGAACATGGAGGATTTTACTTTTAGTTCACTTGTTGATTGTTCTTGAACTATAATTTACTTTTGGACAATCTTTCCTGGTGTTTTCTGGACCTTCATTGTTGTGTTTGGCTGCTGAGAAAATGAAGGAAAATTGAATGACGTCTGGTCTAGGAAAATCTATTCTGCCATTTTTGATCTGAAACTGAGAAATCTATGGGATTCTGATGCAGTTCATGGAATTGAATTTGCATTTGGGGCTCATGAGTATCCAAGTACAGGGATATTTGAAGGGGAACCGAAGCAATGTGATGGATTCAAGTTCAGAGAATCAATTTTGATTGGGAAAACAGATATGAGTGAAGCAGAAGTGAGGTCATTAATGGAGGAACTTGGTAAAGATTACAGAGGAAATGCATATAATCTCATCACCAAAAATTGCAACCATTTTTGCAACCATGTTTGCATCAAACTCACTGGAAATCCCATTCCAAATTGGGTCAATCGCCTTGCTAGAATTGGTAAATTTTGATCTAAATTCACTTCTCCTTTTCTAGTTCTTGCTGTTACTGTTTCTCTTTGAATGCTGCTGATGATGAACTTGACTACTTTTGTAAAAAGAAAATTATTTTGATCCTTTTTTTCTTAAACTAGTTTACAAAAAGTTGCTTGAAATATTCTCTACTCATTTAAGTGGTAGGTATGGAGTTTCATAACAAATTTAAGTGGTAGGTGGCTTGATATCATTTAGCTTTTTTTTTTTCATTCTTTCTTTTTGAAAATTTTGTTGGTTTGCTCAATGGTTTTCTGCGACCAAATTTCAAAAACAAGTTTACATTTTTTGGTATGAATTTCTGAAAATATTGATGATAAAACATAAAAAAATCAAACCACTATGAAGAAAATAAAATGATTAATGATGTTAGCGGTGGGCTTGGACCATTACGAATGGTATCAAAGCCAGACACCGGGCTGTGTGCCAACCCACATAGGTTGGAGAGGGGAACGAAACCTTTCTTATAATGGTGTGAAAACTTCTTCTTAACTAACGCGTTTTAAAACTTTGAGTGAAAGCTCAACGAAGACTAACACTTACTGATGGTAGGTTGAGCTATTATAAATTCGAATCTTCTAATATGTATGTGTCATGGATTGTGTTGAATGTTTGTGTTTGTGAAGAACTTCTGAGATGAGGGGTTTTGGAATGCAGGGTGGATATGCAACTGTGTTTTACCAGCAACCTTAAACTCCACGAAATTTGGACAAAACAGAGCGCAAAAGAAGAGAGGTGAAGAAAAACAAGAAGAAGAAGAAGAAGAAGAGAAGAAGAAGAAGGAATTGGCAAGAAGAATCCAAACAGCTGAAATTTCAGCTTCTAATTCTTCATCTTCTTCTTCTTCATCTTCTTCAACTACTACTCTCCGCAGAGGAAGAAGCAGAACCAGACGTCCTCGTCTTCCAGCTTCACCATTGATACCCACTTCAAGTTCTTGA

mRNA sequence

CAATGGAGTTGTTATTAATCGCAGTAAGCGAGTGGGAGTGAGATCGAAATGTGGTGCAGAAGAATCTCCTGCAATGGCGACTCTGGATCGGTGCCAGTGTACCTCAATGTGTACGACCTGACGCACATTAATGGCTACGCATATTGGCTTGGCCTCGGAGTTTATCATTCTGGTGTACAAGAAAATGAAGGAAAATTGAATGACGTCTGGTCTAGGAAAATCTATTCTGCCATTTTTGATCTGAAACTGAGAAATCTATGGGATTCTGATGCAGTTCATGGAATTGAATTTGCATTTGGGGCTCATGAGTATCCAAGTACAGGGATATTTGAAGGGGAACCGAAGCAATGTGATGGATTCAAGTTCAGAGAATCAATTTTGATTGGGAAAACAGATATGAGTGAAGCAGAAGTGAGGTCATTAATGGAGGAACTTGGTAAAGATTACAGAGGAAATGCATATAATCTCATCACCAAAAATTGCAACCATTTTTGCAACCATGTTTGCATCAAACTCACTGGAAATCCCATTCCAAATTGGGTCAATCGCCTTGCTAGAATTGGGTGGATATGCAACTGTGTTTTACCAGCAACCTTAAACTCCACGAAATTTGGACAAAACAGAGCGCAAAAGAAGAGAGGTGAAGAAAAACAAGAAGAAGAAGAAGAAGAAGAGAAGAAGAAGAAGGAATTGGCAAGAAGAATCCAAACAGCTGAAATTTCAGCTTCTAATTCTTCATCTTCTTCTTCTTCATCTTCTTCAACTACTACTCTCCGCAGAGGAAGAAGCAGAACCAGACGTCCTCGTCTTCCAGCTTCACCATTGATACCCACTTCAAGTTCTTGA

Coding sequence (CDS)

ATGTGGTGCAGAAGAATCTCCTGCAATGGCGACTCTGGATCGGTGCCAGTGTACCTCAATGTGTACGACCTGACGCACATTAATGGCTACGCATATTGGCTTGGCCTCGGAGTTTATCATTCTGGTGTACAAGAAAATGAAGGAAAATTGAATGACGTCTGGTCTAGGAAAATCTATTCTGCCATTTTTGATCTGAAACTGAGAAATCTATGGGATTCTGATGCAGTTCATGGAATTGAATTTGCATTTGGGGCTCATGAGTATCCAAGTACAGGGATATTTGAAGGGGAACCGAAGCAATGTGATGGATTCAAGTTCAGAGAATCAATTTTGATTGGGAAAACAGATATGAGTGAAGCAGAAGTGAGGTCATTAATGGAGGAACTTGGTAAAGATTACAGAGGAAATGCATATAATCTCATCACCAAAAATTGCAACCATTTTTGCAACCATGTTTGCATCAAACTCACTGGAAATCCCATTCCAAATTGGGTCAATCGCCTTGCTAGAATTGGGTGGATATGCAACTGTGTTTTACCAGCAACCTTAAACTCCACGAAATTTGGACAAAACAGAGCGCAAAAGAAGAGAGGTGAAGAAAAACAAGAAGAAGAAGAAGAAGAAGAGAAGAAGAAGAAGGAATTGGCAAGAAGAATCCAAACAGCTGAAATTTCAGCTTCTAATTCTTCATCTTCTTCTTCTTCATCTTCTTCAACTACTACTCTCCGCAGAGGAAGAAGCAGAACCAGACGTCCTCGTCTTCCAGCTTCACCATTGATACCCACTTCAAGTTCTTGA

Protein sequence

MWCRRISCNGDSGSVPVYLNVYDLTHINGYAYWLGLGVYHSGVQENEGKLNDVWSRKIYSAIFDLKLRNLWDSDAVHGIEFAFGAHEYPSTGIFEGEPKQCDGFKFRESILIGKTDMSEAEVRSLMEELGKDYRGNAYNLITKNCNHFCNHVCIKLTGNPIPNWVNRLARIGWICNCVLPATLNSTKFGQNRAQKKRGEEKQEEEEEEEKKKKELARRIQTAEISASNSSSSSSSSSSTTTLRRGRSRTRRPRLPASPLIPTSSS

Homology

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BLAST of Carg06534 vs. NCBI nr
Match: KAG7018383.1 (DeSI-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 530.8 bits (1366), Expect = 6.8e-147
Identity = 265/265 (100.00%), Postives = 265/265 (100.00%), Query Frame = 0


Query: 1   MWCRRISCNGDSGSVPVYLNVYDLTHINGYAYWLGLGVYHSGVQENEGKLNDVWSRKIYS 60
           MWCRRISCNGDSGSVPVYLNVYDLTHINGYAYWLGLGVYHSGVQENEGKLNDVWSRKIYS
Sbjct: 1   MWCRRISCNGDSGSVPVYLNVYDLTHINGYAYWLGLGVYHSGVQENEGKLNDVWSRKIYS 60

Query: 61  AIFDLKLRNLWDSDAVHGIEFAFGAHEYPSTGIFEGEPKQCDGFKFRESILIGKTDMSEA 120
           AIFDLKLRNLWDSDAVHGIEFAFGAHEYPSTGIFEGEPKQCDGFKFRESILIGKTDMSEA
Sbjct: 61  AIFDLKLRNLWDSDAVHGIEFAFGAHEYPSTGIFEGEPKQCDGFKFRESILIGKTDMSEA 120

Query: 121 EVRSLMEELGKDYRGNAYNLITKNCNHFCNHVCIKLTGNPIPNWVNRLARIGWICNCVLP 180
           EVRSLMEELGKDYRGNAYNLITKNCNHFCNHVCIKLTGNPIPNWVNRLARIGWICNCVLP
Sbjct: 121 EVRSLMEELGKDYRGNAYNLITKNCNHFCNHVCIKLTGNPIPNWVNRLARIGWICNCVLP 180

Query: 181 ATLNSTKFGQNRAQKKRGEEKQEEEEEEEKKKKELARRIQTAEISASNSSSSSSSSSSTT 240
           ATLNSTKFGQNRAQKKRGEEKQEEEEEEEKKKKELARRIQTAEISASNSSSSSSSSSSTT
Sbjct: 181 ATLNSTKFGQNRAQKKRGEEKQEEEEEEEKKKKELARRIQTAEISASNSSSSSSSSSSTT 240

Query: 241 TLRRGRSRTRRPRLPASPLIPTSSS 266
           TLRRGRSRTRRPRLPASPLIPTSSS
Sbjct: 241 TLRRGRSRTRRPRLPASPLIPTSSS 265

[Close]

BLAST of Carg06534 vs. NCBI nr
Match: KAG6581964.1 (DeSI-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 441.4 bits (1134), Expect = 5.4e-120
Identity = 232/265 (87.55%), Postives = 232/265 (87.55%), Query Frame = 0


Query: 1   MWCRRISCNGDSGSVPVYLNVYDLTHINGYAYWLGLGVYHSGVQENEGKLNDVWSRKIYS 60
           MWCRRISCNGDSGSVPVYLNVYDLTHINGYAYWLGLGVYHSGVQ                
Sbjct: 1   MWCRRISCNGDSGSVPVYLNVYDLTHINGYAYWLGLGVYHSGVQ---------------- 60

Query: 61  AIFDLKLRNLWDSDAVHGIEFAFGAHEYPSTGIFEGEPKQCDGFKFRESILIGKTDMSEA 120
                          VHGIEFAFGAHEYPSTGIFEGEPKQCDGFKFRESILIGKTDMSEA
Sbjct: 61  ---------------VHGIEFAFGAHEYPSTGIFEGEPKQCDGFKFRESILIGKTDMSEA 120

Query: 121 EVRSLMEELGKDYRGNAYNLITKNCNHFCNHVCIKLTGNPIPNWVNRLARIGWICNCVLP 180
           EVRSLMEELGKDYRGNAYNLITKNCNHFCNHVCIKLTGNPIPNWVNRLARIGWICNCVLP
Sbjct: 121 EVRSLMEELGKDYRGNAYNLITKNCNHFCNHVCIKLTGNPIPNWVNRLARIGWICNCVLP 180

Query: 181 ATLNSTKFGQNRAQKKRGEEKQEEEEEEEKKKKELARRIQTAEISASNSSSSSSSSSSTT 240
           ATLNSTKFGQNRAQKKRGEEKQ  EEEEEKKKKELARRIQTAEISASNSSSSSSSSSSTT
Sbjct: 181 ATLNSTKFGQNRAQKKRGEEKQ--EEEEEKKKKELARRIQTAEISASNSSSSSSSSSSTT 232

Query: 241 TLRRGRSRTRRPRLPASPLIPTSSS 266
           TLRRGRSRTRRPRLPASPLIPTSSS
Sbjct: 241 TLRRGRSRTRRPRLPASPLIPTSSS 232

[Close]

BLAST of Carg06534 vs. NCBI nr
Match: XP_023528854.1 (deSI-like protein At4g17486 [Cucurbita pepo subsp. pepo] >XP_023528855.1 deSI-like protein At4g17486 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 435.6 bits (1119), Expect = 3.0e-118
Identity = 229/262 (87.40%), Postives = 229/262 (87.40%), Query Frame = 0


Query: 1   MWCRRISCNGDSGSVPVYLNVYDLTHINGYAYWLGLGVYHSGVQENEGKLNDVWSRKIYS 60
           MWCRRISCNGDSGSVPVYLNVYDLTHINGYAYWLGLGVYHSGVQ                
Sbjct: 1   MWCRRISCNGDSGSVPVYLNVYDLTHINGYAYWLGLGVYHSGVQ---------------- 60

Query: 61  AIFDLKLRNLWDSDAVHGIEFAFGAHEYPSTGIFEGEPKQCDGFKFRESILIGKTDMSEA 120
                          VHGIEFAFGAHEYPSTGIFEGEPKQCDGFKFRESILIGKTDMSEA
Sbjct: 61  ---------------VHGIEFAFGAHEYPSTGIFEGEPKQCDGFKFRESILIGKTDMSEA 120

Query: 121 EVRSLMEELGKDYRGNAYNLITKNCNHFCNHVCIKLTGNPIPNWVNRLARIGWICNCVLP 180
           EVRSLMEELGKDYRGNAYNLITKNCNHFCN VCIKLTGNPIPNWVNRLARIGWICNCVLP
Sbjct: 121 EVRSLMEELGKDYRGNAYNLITKNCNHFCNRVCIKLTGNPIPNWVNRLARIGWICNCVLP 180

Query: 181 ATLNSTKFGQNRAQKKRGEEKQEEEEEEEKKKKELARRIQTAEISASNSSSSSSSSSSTT 240
           ATLNSTKFGQNRAQKKRGEEKQEEEEEEE KKKELARRIQTAEISASNSSSSSSSSSSTT
Sbjct: 181 ATLNSTKFGQNRAQKKRGEEKQEEEEEEE-KKKELARRIQTAEISASNSSSSSSSSSSTT 230

Query: 241 TLRRGRSRTRRPRLPASPLIPT 263
           TLRRGRSRTRRPRLPASPLIPT
Sbjct: 241 TLRRGRSRTRRPRLPASPLIPT 230

[Close]

BLAST of Carg06534 vs. NCBI nr
Match: XP_022955511.1 (deSI-like protein At4g17486 [Cucurbita moschata])

HSP 1 Score: 431.4 bits (1108), Expect = 5.6e-117
Identity = 228/265 (86.04%), Postives = 228/265 (86.04%), Query Frame = 0


Query: 1   MWCRRISCNGDSGSVPVYLNVYDLTHINGYAYWLGLGVYHSGVQENEGKLNDVWSRKIYS 60
           MWCRRISCNGDSGSVPVYLNVYDLTHINGYAYWLGLGVYHSGVQ                
Sbjct: 1   MWCRRISCNGDSGSVPVYLNVYDLTHINGYAYWLGLGVYHSGVQ---------------- 60

Query: 61  AIFDLKLRNLWDSDAVHGIEFAFGAHEYPSTGIFEGEPKQCDGFKFRESILIGKTDMSEA 120
                          VHGIEFAFGAHEYPSTGIFEGEPKQCDGFKFRESILIGKTDMSEA
Sbjct: 61  ---------------VHGIEFAFGAHEYPSTGIFEGEPKQCDGFKFRESILIGKTDMSEA 120

Query: 121 EVRSLMEELGKDYRGNAYNLITKNCNHFCNHVCIKLTGNPIPNWVNRLARIGWICNCVLP 180
           EVRSLMEELGKDYRGNAYNLITKNCNHFCNHVCIKLTGNPIPNWVNRLARIGWICNCVLP
Sbjct: 121 EVRSLMEELGKDYRGNAYNLITKNCNHFCNHVCIKLTGNPIPNWVNRLARIGWICNCVLP 180

Query: 181 ATLNSTKFGQNRAQKKRGEEKQEEEEEEEKKKKELARRIQTAEISASNSSSSSSSSSSTT 240
           ATLNSTKFGQNRAQKKRGEEKQ EEEEEEKKKKELARRIQTAEISASN     SSSSSTT
Sbjct: 181 ATLNSTKFGQNRAQKKRGEEKQ-EEEEEEKKKKELARRIQTAEISASN-----SSSSSTT 228

Query: 241 TLRRGRSRTRRPRLPASPLIPTSSS 266
           TLRRGRSRTRRPRLPASPLIPTSSS
Sbjct: 241 TLRRGRSRTRRPRLPASPLIPTSSS 228

[Close]

BLAST of Carg06534 vs. NCBI nr
Match: XP_022980803.1 (deSI-like protein At4g17486 [Cucurbita maxima])

HSP 1 Score: 428.3 bits (1100), Expect = 4.7e-116
Identity = 228/265 (86.04%), Postives = 229/265 (86.42%), Query Frame = 0


Query: 1   MWCRRISCNGDSGSVPVYLNVYDLTHINGYAYWLGLGVYHSGVQENEGKLNDVWSRKIYS 60
           MWCRRISCNGDSGSVPVYLNVYDLTHINGYAYWLGLGVYHSGVQ                
Sbjct: 1   MWCRRISCNGDSGSVPVYLNVYDLTHINGYAYWLGLGVYHSGVQ---------------- 60

Query: 61  AIFDLKLRNLWDSDAVHGIEFAFGAHEYPSTGIFEGEPKQCDGFKFRESILIGKTDMSEA 120
                          VHGIEFAFGAHE PSTGIFEGEPKQCDGFKFRESILIGKTDMSEA
Sbjct: 61  ---------------VHGIEFAFGAHECPSTGIFEGEPKQCDGFKFRESILIGKTDMSEA 120

Query: 121 EVRSLMEELGKDYRGNAYNLITKNCNHFCNHVCIKLTGNPIPNWVNRLARIGWICNCVLP 180
           EVRSLMEELGKDYRGNAYNLITKNCNHFCNH CIKLTGNPIP+WVNRLARIGWICNCVLP
Sbjct: 121 EVRSLMEELGKDYRGNAYNLITKNCNHFCNHFCIKLTGNPIPSWVNRLARIGWICNCVLP 180

Query: 181 ATLNSTKFGQNRAQKKRGEEKQEEEEEEEKKKKELARRIQTAEISASNSSSSSSSSSSTT 240
           ATLNSTKFGQNRAQKKRGEEKQEEEEEE  KKKELARRIQTAEISASN SSSSSSSSSTT
Sbjct: 181 ATLNSTKFGQNRAQKKRGEEKQEEEEEE--KKKELARRIQTAEISASN-SSSSSSSSSTT 231

Query: 241 TLRRGRSRTRRPRLPASPLIPTSSS 266
           TLRRGRSRTRRPRLPASPLIPTSSS
Sbjct: 241 TLRRGRSRTRRPRLPASPLIPTSSS 231

[Close]

BLAST of Carg06534 vs. ExPASy Swiss-Prot
Match: Q93VG8 (DeSI-like protein At4g17486 OS=Arabidopsis thaliana OX=3702 GN=At4g17486 PE=2 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 1.6e-45
Identity = 87/205 (42.44%), Postives = 121/205 (59.02%), Query Frame = 0


Query: 5   RISCNGDSGSVPVYLNVYDLTHINGYAYWLGLGVYHSGVQENEGKLNDVWSRKIYSAIFD 64
           R   +G++   PVYLNVYDLT +N Y YW G+G++HSG++                    
Sbjct: 16  RDESSGEAALTPVYLNVYDLTPVNNYLYWFGIGIFHSGIE-------------------- 75

Query: 65  LKLRNLWDSDAVHGIEFAFGAHEYPSTGIFEGEPKQCDGFKFRESILIGKTDMSEAEVRS 124
                       H +E+ +GAHEYP++G++E EP+ C GF FR S+L+G T MS ++ RS
Sbjct: 76  -----------AHNLEYCYGAHEYPTSGVYEVEPRNCPGFIFRRSVLLGTTSMSRSDFRS 135

Query: 125 LMEELGKDYRGNAYNLITKNCNHFCNHVCIKLTGNPIPNWVNRLARIGWICNCVLPATLN 184
            ME+L + Y G+ Y+LI KNCNHF   VC++LTG PIP W+NRLAR+G  CNC+LP ++ 
Sbjct: 136 YMEKLSRKYHGDTYHLIAKNCNHFTEEVCLQLTGKPIPGWINRLARVGSFCNCLLPESIQ 186

Query: 185 STKFGQNRAQKKRGEEKQEEEEEEE 210
            T      A  +R E   E+E   E
Sbjct: 196 LTAVS---ALPERLEFSDEDESNSE 186

[Close]

BLAST of Carg06534 vs. ExPASy Swiss-Prot
Match: Q6DC39 (Deubiquitinase DESI2 OS=Danio rerio OX=7955 GN=desi2 PE=2 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 8.6e-28
Identity = 71/198 (35.86%), Postives = 109/198 (55.05%), Query Frame = 0


Query: 16  PVYLNVYDLTHINGYAYWLGLGVYHSGVQENEGKLNDVWSRKIYSAIFDLKLRNLWDSDA 75
           PV LNVYD+  IN +   LG+GV+HSG++                               
Sbjct: 5   PVILNVYDMYWINEFTSSLGIGVFHSGIE------------------------------- 64

Query: 76  VHGIEFAFGAHEYPSTGIFEGEPKQC----DGFKFRESILIGKTDMSEAEVRSLMEELGK 135
           ++G EFA+G H YP +GIFE  P       + FKF+E+I++G TD +E +V  ++EE+GK
Sbjct: 65  IYGREFAYGGHPYPFSGIFEITPGDATELGETFKFKEAIVLGSTDFTEEDVERIVEEMGK 124

Query: 136 DYRGNAYNLITKNCNHFCNHVCIKLTGNPIPNWVNRLAR----IGWICNCVLPATLNSTK 195
           +Y+GNAY+L+ KNCNHF + +   L G  IP WVNRLA     + ++ +C+    L    
Sbjct: 125 EYKGNAYHLMHKNCNHFSSALSEILCGREIPRWVNRLAYFSSCVPFLQSCLPKEWLTPAA 171

Query: 196 FGQNRAQKKRGEEKQEEE 206
              + +Q+ +GE ++ E+
Sbjct: 185 LQSSVSQELQGELEEAED 171

[Close]

BLAST of Carg06534 vs. ExPASy Swiss-Prot
Match: Q5PQ09 (Deubiquitinase DESI2 OS=Xenopus laevis OX=8355 GN=desi2 PE=2 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 1.2e-26
Identity = 84/235 (35.74%), Postives = 116/235 (49.36%), Query Frame = 0


Query: 16  PVYLNVYDLTHINGYAYWLGLGVYHSGVQENEGKLNDVWSRKIYSAIFDLKLRNLWDSDA 75
           P+ LNVYD+  IN Y   LG+GV+HSG+Q                               
Sbjct: 5   PIILNVYDMYWINEYTSSLGIGVFHSGIQ------------------------------- 64

Query: 76  VHGIEFAFGAHEYPSTGIFEGEPKQC----DGFKFRESILIGKTDMSEAEVRSLMEELGK 135
           V+G EFA+G H YP +G+FE  P       D FKF+E+I +G TD +E ++  ++EELGK
Sbjct: 65  VYGREFAYGGHPYPFSGVFEISPGDSTELGDTFKFKEAIALGSTDFTENDIEKIIEELGK 124

Query: 136 DYRGNAYNLITKNCNHFCNHVCIKLTGNPIPNWVNRLARIGWICNCVLPATLNSTKFGQN 195
           +Y+GNAY+L+ KNCNHF + +   L G  IP WVNRLA     C   L + L        
Sbjct: 125 EYKGNAYHLMHKNCNHFSSALSEILCGKEIPRWVNRLAYFS-TCVPFLQSCLPKEWLTPA 184

Query: 196 RAQKKRGEEKQEEEEEEEKKKKELARRIQTAEISASNSSSSSSSSSSTTTLRRGR 247
             Q    +E Q+E EE E                    +++S+S+S+T   R GR
Sbjct: 185 ALQSSISQELQDELEEAE-------------------DAAASASTSTTAMPRPGR 188

[Close]

BLAST of Carg06534 vs. ExPASy Swiss-Prot
Match: Q9BSY9 (Deubiquitinase DESI2 OS=Homo sapiens OX=9606 GN=DESI2 PE=1 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 8.1e-26
Identity = 72/199 (36.18%), Postives = 105/199 (52.76%), Query Frame = 0


Query: 17  VYLNVYDLTHINGYAYWLGLGVYHSGVQENEGKLNDVWSRKIYSAIFDLKLRNLWDSDAV 76
           V LNVYD+  +N Y   +G+GV+HSG++                               V
Sbjct: 7   VVLNVYDMYWMNEYTSSIGIGVFHSGIE-------------------------------V 66

Query: 77  HGIEFAFGAHEYPSTGIFEGEPKQC----DGFKFRESILIGKTDMSEAEVRSLMEELGKD 136
           +G EFA+G H YP +GIFE  P       + FKF+E++++G TD  E ++  ++EELGK+
Sbjct: 67  YGREFAYGGHPYPFSGIFEISPGNASELGETFKFKEAVVLGSTDFLEDDIEKIVEELGKE 126

Query: 137 YRGNAYNLITKNCNHFCNHVCIKLTGNPIPNWVNRLARIGWICNCV--LPATLNSTKFGQ 196
           Y+GNAY+L+ KNCNHF + +   L G  IP W+NRLA   +  +C+  L + L       
Sbjct: 127 YKGNAYHLMHKNCNHFSSALSEILCGKEIPRWINRLA---YFSSCIPFLQSCLPKEWLTP 171

Query: 197 NRAQKKRGEEKQEEEEEEE 210
              Q    +E Q+E EE E
Sbjct: 187 AALQSSVSQELQDELEEAE 171

[Close]

BLAST of Carg06534 vs. ExPASy Swiss-Prot
Match: Q5XIT6 (Deubiquitinase DESI2 OS=Rattus norvegicus OX=10116 GN=Desi2 PE=2 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 8.1e-26
Identity = 72/199 (36.18%), Postives = 105/199 (52.76%), Query Frame = 0


Query: 17  VYLNVYDLTHINGYAYWLGLGVYHSGVQENEGKLNDVWSRKIYSAIFDLKLRNLWDSDAV 76
           V LNVYD+  +N Y   +G+GV+HSG++                               V
Sbjct: 7   VVLNVYDMYWMNEYTSSIGIGVFHSGIE-------------------------------V 66

Query: 77  HGIEFAFGAHEYPSTGIFEGEPKQC----DGFKFRESILIGKTDMSEAEVRSLMEELGKD 136
           +G EFA+G H YP +GIFE  P       + FKF+E++++G TD  E ++  ++EELGK+
Sbjct: 67  YGREFAYGGHPYPFSGIFEISPGNAAELGETFKFKEAVVLGSTDFLEDDIEKIVEELGKE 126

Query: 137 YRGNAYNLITKNCNHFCNHVCIKLTGNPIPNWVNRLARIGWICNCV--LPATLNSTKFGQ 196
           Y+GNAY+L+ KNCNHF + +   L G  IP W+NRLA   +  +C+  L + L       
Sbjct: 127 YKGNAYHLMHKNCNHFSSALSEILCGKEIPRWINRLA---YFSSCIPFLQSCLPKEWLTP 171

Query: 197 NRAQKKRGEEKQEEEEEEE 210
              Q    +E Q+E EE E
Sbjct: 187 AALQSSVSQELQDELEEAE 171

[Close]

BLAST of Carg06534 vs. ExPASy TrEMBL
Match: A0A6J1GTY9 (deSI-like protein At4g17486 OS=Cucurbita moschata OX=3662 GN=LOC111457516 PE=3 SV=1)

HSP 1 Score: 431.4 bits (1108), Expect = 2.7e-117
Identity = 228/265 (86.04%), Postives = 228/265 (86.04%), Query Frame = 0


Query: 1   MWCRRISCNGDSGSVPVYLNVYDLTHINGYAYWLGLGVYHSGVQENEGKLNDVWSRKIYS 60
           MWCRRISCNGDSGSVPVYLNVYDLTHINGYAYWLGLGVYHSGVQ                
Sbjct: 1   MWCRRISCNGDSGSVPVYLNVYDLTHINGYAYWLGLGVYHSGVQ---------------- 60

Query: 61  AIFDLKLRNLWDSDAVHGIEFAFGAHEYPSTGIFEGEPKQCDGFKFRESILIGKTDMSEA 120
                          VHGIEFAFGAHEYPSTGIFEGEPKQCDGFKFRESILIGKTDMSEA
Sbjct: 61  ---------------VHGIEFAFGAHEYPSTGIFEGEPKQCDGFKFRESILIGKTDMSEA 120

Query: 121 EVRSLMEELGKDYRGNAYNLITKNCNHFCNHVCIKLTGNPIPNWVNRLARIGWICNCVLP 180
           EVRSLMEELGKDYRGNAYNLITKNCNHFCNHVCIKLTGNPIPNWVNRLARIGWICNCVLP
Sbjct: 121 EVRSLMEELGKDYRGNAYNLITKNCNHFCNHVCIKLTGNPIPNWVNRLARIGWICNCVLP 180

Query: 181 ATLNSTKFGQNRAQKKRGEEKQEEEEEEEKKKKELARRIQTAEISASNSSSSSSSSSSTT 240
           ATLNSTKFGQNRAQKKRGEEKQ EEEEEEKKKKELARRIQTAEISASN     SSSSSTT
Sbjct: 181 ATLNSTKFGQNRAQKKRGEEKQ-EEEEEEKKKKELARRIQTAEISASN-----SSSSSTT 228

Query: 241 TLRRGRSRTRRPRLPASPLIPTSSS 266
           TLRRGRSRTRRPRLPASPLIPTSSS
Sbjct: 241 TLRRGRSRTRRPRLPASPLIPTSSS 228

[Close]

BLAST of Carg06534 vs. ExPASy TrEMBL
Match: A0A6J1J0C0 (deSI-like protein At4g17486 OS=Cucurbita maxima OX=3661 GN=LOC111480086 PE=3 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 2.3e-116
Identity = 228/265 (86.04%), Postives = 229/265 (86.42%), Query Frame = 0


Query: 1   MWCRRISCNGDSGSVPVYLNVYDLTHINGYAYWLGLGVYHSGVQENEGKLNDVWSRKIYS 60
           MWCRRISCNGDSGSVPVYLNVYDLTHINGYAYWLGLGVYHSGVQ                
Sbjct: 1   MWCRRISCNGDSGSVPVYLNVYDLTHINGYAYWLGLGVYHSGVQ---------------- 60

Query: 61  AIFDLKLRNLWDSDAVHGIEFAFGAHEYPSTGIFEGEPKQCDGFKFRESILIGKTDMSEA 120
                          VHGIEFAFGAHE PSTGIFEGEPKQCDGFKFRESILIGKTDMSEA
Sbjct: 61  ---------------VHGIEFAFGAHECPSTGIFEGEPKQCDGFKFRESILIGKTDMSEA 120

Query: 121 EVRSLMEELGKDYRGNAYNLITKNCNHFCNHVCIKLTGNPIPNWVNRLARIGWICNCVLP 180
           EVRSLMEELGKDYRGNAYNLITKNCNHFCNH CIKLTGNPIP+WVNRLARIGWICNCVLP
Sbjct: 121 EVRSLMEELGKDYRGNAYNLITKNCNHFCNHFCIKLTGNPIPSWVNRLARIGWICNCVLP 180

Query: 181 ATLNSTKFGQNRAQKKRGEEKQEEEEEEEKKKKELARRIQTAEISASNSSSSSSSSSSTT 240
           ATLNSTKFGQNRAQKKRGEEKQEEEEEE  KKKELARRIQTAEISASN SSSSSSSSSTT
Sbjct: 181 ATLNSTKFGQNRAQKKRGEEKQEEEEEE--KKKELARRIQTAEISASN-SSSSSSSSSTT 231

Query: 241 TLRRGRSRTRRPRLPASPLIPTSSS 266
           TLRRGRSRTRRPRLPASPLIPTSSS
Sbjct: 241 TLRRGRSRTRRPRLPASPLIPTSSS 231

[Close]

BLAST of Carg06534 vs. ExPASy TrEMBL
Match: A0A0A0KX01 (PPPDE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G006490 PE=3 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 1.5e-104
Identity = 205/266 (77.07%), Postives = 217/266 (81.58%), Query Frame = 0


Query: 1   MWCRRISCNGDSGSVPVYLNVYDLTHINGYAYWLGLGVYHSGVQENEGKLNDVWSRKIYS 60
           M CRRIS N DSGS PVYLNVYDLT INGYAYW GLGVYHSG+Q                
Sbjct: 1   MLCRRISSNADSGSAPVYLNVYDLTPINGYAYWFGLGVYHSGLQ---------------- 60

Query: 61  AIFDLKLRNLWDSDAVHGIEFAFGAHEYPSTGIFEGEPKQCDGFKFRESILIGKTDMSEA 120
                          VHGIEFAFGAHEYPSTGIFEGEPKQC+GFKFR+SILIG+TD+SEA
Sbjct: 61  ---------------VHGIEFAFGAHEYPSTGIFEGEPKQCEGFKFRKSILIGQTDLSEA 120

Query: 121 EVRSLMEELGKDYRGNAYNLITKNCNHFCNHVCIKLTGNPIPNWVNRLARIGWICNCVLP 180
           EV+SLMEELGKDYRGNAYNLITKNCNHFCNHVCIKLTGNPIP+WVNRLARIGWICNCVLP
Sbjct: 121 EVKSLMEELGKDYRGNAYNLITKNCNHFCNHVCIKLTGNPIPSWVNRLARIGWICNCVLP 180

Query: 181 ATLNSTKFGQNRAQKKRGEEKQEE--EEEEEKKKKELARRIQTAEISASNSSSSSSSSSS 240
           ATLNSTKFGQNR QKK  EEKQE+  EEEEEKKKKEL  ++QT EIS SNS+SSSSSSSS
Sbjct: 181 ATLNSTKFGQNRVQKKTNEEKQEKGVEEEEEKKKKELMPKVQTVEISGSNSNSSSSSSSS 235

Query: 241 TTTLRRGRSRTRRPRLPASPLIPTSS 265
             TLRRGRSRTRRPRLP SPLIPTS+
Sbjct: 241 PITLRRGRSRTRRPRLPTSPLIPTSN 235

[Close]

BLAST of Carg06534 vs. ExPASy TrEMBL
Match: A0A1S3BXH1 (deSI-like protein At4g17486 OS=Cucumis melo OX=3656 GN=LOC103494655 PE=3 SV=1)

HSP 1 Score: 374.0 bits (959), Expect = 5.1e-100
Identity = 200/266 (75.19%), Postives = 215/266 (80.83%), Query Frame = 0


Query: 1   MWCRRISCNGDSGSVPVYLNVYDLTHINGYAYWLGLGVYHSGVQENEGKLNDVWSRKIYS 60
           M CRRIS N DSGS PVYLNVYDLT INGYAYW GLGVYHSG+Q                
Sbjct: 1   MLCRRISSNADSGSAPVYLNVYDLTPINGYAYWFGLGVYHSGLQ---------------- 60

Query: 61  AIFDLKLRNLWDSDAVHGIEFAFGAHEYPSTGIFEGEPKQCDGFKFRESILIGKTDMSEA 120
                          VHGIEFAFGAHEYPSTGIFEGEPKQC+GF+FR+SILIGKTD+SEA
Sbjct: 61  ---------------VHGIEFAFGAHEYPSTGIFEGEPKQCEGFRFRKSILIGKTDLSEA 120

Query: 121 EVRSLMEELGKDYRGNAYNLITKNCNHFCNHVCIKLTGNPIPNWVNRLARIGWICNCVLP 180
           EV+SLMEELGKDYRGNAYNLITKNCNHFCNHVCIKLTGNPIP+WVNRLARIGWICNCVLP
Sbjct: 121 EVKSLMEELGKDYRGNAYNLITKNCNHFCNHVCIKLTGNPIPSWVNRLARIGWICNCVLP 180

Query: 181 ATLNSTKFGQNRAQKKRGEEKQEE-EEEEEKKKKELARRIQTAEISASNSSSSSSSSSST 240
           ATL+ST+FGQNR QKK  EEKQE+  EEEE+KKKE   +IQT EIS S+ S+SSSSSSS 
Sbjct: 181 ATLSSTRFGQNRVQKKTNEEKQEKGGEEEEEKKKESMPKIQTVEISGSH-SNSSSSSSSP 234

Query: 241 TTLRRGRSRTRRPRLPASPLIPTSSS 266
            TLRRGRSRTRRPRLP SPLIPTS+S
Sbjct: 241 ITLRRGRSRTRRPRLPTSPLIPTSNS 234

[Close]

BLAST of Carg06534 vs. ExPASy TrEMBL
Match: A0A6J1DIV3 (deSI-like protein At4g17486 OS=Momordica charantia OX=3673 GN=LOC111020936 PE=3 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 9.7e-99
Identity = 202/265 (76.23%), Postives = 211/265 (79.62%), Query Frame = 0


Query: 1   MWCRRISCNGDSGSVPVYLNVYDLTHINGYAYWLGLGVYHSGVQENEGKLNDVWSRKIYS 60
           M CRRIS NGDSGS PVYLNVYDLT INGYAYW GLGVYHSGVQ                
Sbjct: 1   MLCRRISSNGDSGSAPVYLNVYDLTPINGYAYWFGLGVYHSGVQ---------------- 60

Query: 61  AIFDLKLRNLWDSDAVHGIEFAFGAHEYPSTGIFEGEPKQCDGFKFRESILIGKTDMSEA 120
                          VHGIEFAFGAHEYPSTGIFEGEPKQCDGFKFR SILIGKTD+S+A
Sbjct: 61  ---------------VHGIEFAFGAHEYPSTGIFEGEPKQCDGFKFRRSILIGKTDLSQA 120

Query: 121 EVRSLMEELGKDYRGNAYNLITKNCNHFCNHVCIKLTGNPIPNWVNRLARIGWICNCVLP 180
           EVRSLMEELGKDYRGNAYNLITKNCNHFCN VC+KLTGN IP+WVNRLARIGWICNCVLP
Sbjct: 121 EVRSLMEELGKDYRGNAYNLITKNCNHFCNQVCMKLTGNSIPSWVNRLARIGWICNCVLP 180

Query: 181 ATLNSTKFGQNRAQKKRGEEKQEEEEEEEKKKKELARRIQTAEISASNSSSSSSSSSSTT 240
           ATLNSTKFGQNR +KK  E   EEEEEEEKKKKE +  +QTAEI+ASN  SSSSSSSS T
Sbjct: 181 ATLNSTKFGQNRVKKKTSE---EEEEEEEKKKKE-SPIVQTAEIAASN--SSSSSSSSPT 228

Query: 241 TLRRGRSRTRRPRLPASPLIPTSSS 266
            LRRGRSRTRRPR P SPLIPTS+S
Sbjct: 241 ILRRGRSRTRRPRPPPSPLIPTSNS 228

[Close]

BLAST of Carg06534 vs. TAIR 10
Match: AT1G80690.1 (PPPDE putative thiol peptidase family protein )

HSP 1 Score: 273.5 bits (698), Expect = 1.8e-73
Identity = 153/267 (57.30%), Postives = 183/267 (68.54%), Query Frame = 0


Query: 1   MWCRRISCNGDSGSVPVYLNVYDLTHINGYAYWLGLGVYHSGVQENEGKLNDVWSRKIYS 60
           M CR+ S   D G+VPVYLNVYDLT INGYAYWLGLGVYHSGV+                
Sbjct: 1   MLCRKNSSLVDRGNVPVYLNVYDLTPINGYAYWLGLGVYHSGVE---------------- 60

Query: 61  AIFDLKLRNLWDSDAVHGIEFAFGAHEYPSTGIFEGEPKQCDGFKFRESILIGKTDMSEA 120
                          VHGIE+A+GAHEYPSTGIFEGEPKQC+GF FR+SILIGKTD+   
Sbjct: 61  ---------------VHGIEYAYGAHEYPSTGIFEGEPKQCEGFTFRKSILIGKTDLGPL 120

Query: 121 EVRSLMEELGKDYRGNAYNLITKNCNHFCNHVCIKLTGNPIPNWVNRLARIGWICNCVLP 180
           EVR+ ME+L  +Y+G++YNLITKNCNHFC+  CIKLTGNPIP+WVNRLARIG++CNCVLP
Sbjct: 121 EVRATMEQLADNYKGSSYNLITKNCNHFCDETCIKLTGNPIPSWVNRLARIGFMCNCVLP 180

Query: 181 ATLNSTKFGQNRAQKKRGEEKQEEEEEEEKKKKELARRIQTAEISASNSSSSSSSSSSTT 240
           AT+N+T+FG NR      ++K  E E E+KK   ++ R        S  ++ SSSSSS +
Sbjct: 181 ATINATRFGNNRV----NQDKSCEAENEKKKLTSVSSR------ERSTIATPSSSSSSPS 226

Query: 241 TLRRGRSRTRRPRL--PASPLIPTSSS 266
              RGRSR RRPR   P+SPL   SSS
Sbjct: 241 VQVRGRSRKRRPRALQPSSPLTLGSSS 226

[Close]

BLAST of Carg06534 vs. TAIR 10
Match: AT5G25170.1 (PPPDE putative thiol peptidase family protein )

HSP 1 Score: 220.3 bits (560), Expect = 1.8e-57
Identity = 129/263 (49.05%), Postives = 156/263 (59.32%), Query Frame = 0


Query: 1   MWCRRISCNG---DSGSVPVYLNVYDLTHINGYAYWLGLGVYHSGVQENEGKLNDVWSRK 60
           M CR +   G     GSVPVYLNVYDLT INGYAYWLGLG+YHSGV+             
Sbjct: 1   MLCRMVVVTGRKKKPGSVPVYLNVYDLTPINGYAYWLGLGIYHSGVE------------- 60

Query: 61  IYSAIFDLKLRNLWDSDAVHGIEFAFGAHEYPSTGIFEGEPKQCDGFKFRESILIGKTDM 120
                             VHG+E+ FGAH++ +TGIFE EPKQC GF FR+SILIG+TD+
Sbjct: 61  ------------------VHGVEYGFGAHDHSTTGIFEVEPKQCPGFTFRKSILIGRTDL 120

Query: 121 SEAEVRSLMEELGKDYRGNAYNLITKNCNHFCNHVCIKLTGNPIPNWVNRLARIGWICNC 180
               VR  ME+L ++Y GN+Y+LITKNCNHFCN VC++LT   IP+WVNRLAR G  CNC
Sbjct: 121 DPENVRVFMEKLAEEYSGNSYHLITKNCNHFCNDVCVQLTRRSIPSWVNRLARFGLFCNC 180

Query: 181 VLPATLNSTKFGQNRAQKKRGEEKQEEEEEEEKKKKELARRIQTAEISASNSSSSSSSSS 240
           VLPA LN TK  Q R+         +EE+  E +KK+L  R       +S      S SS
Sbjct: 181 VLPAELNETKVRQVRS---------KEEKIPEVEKKKLRSR-------SSRFPPGPSLSS 216

Query: 241 STTTLRRGRSRTRRPRLPASPLI 261
           S +  R  R   RR  LP SP +
Sbjct: 241 SGSLNRSRRGERRRQCLPPSPTV 216

[Close]

BLAST of Carg06534 vs. TAIR 10
Match: AT4G31980.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF247, plant (InterPro:IPR004158), Protein of unknown function DUF862, eukaryotic (InterPro:IPR008580); BEST Arabidopsis thaliana protein match is: Plant protein of unknown function (DUF247) (TAIR:AT5G11290.1); Has 1967 Blast hits to 1844 proteins in 183 species: Archae - 0; Bacteria - 6; Metazoa - 223; Fungi - 83; Plants - 1477; Viruses - 0; Other Eukaryotes - 178 (source: NCBI BLink). )

HSP 1 Score: 218.0 bits (554), Expect = 9.1e-57
Identity = 125/263 (47.53%), Postives = 167/263 (63.50%), Query Frame = 0


Query: 1   MWCRRISC--NGDSGSVPVYLNVYDLTHINGYAYWLGLGVYHSGVQENEGKLNDVWSRKI 60
           M+CR +S     +SG+VPVYLNVYDLT +N Y YWLG+G+YHSG++              
Sbjct: 1   MFCRNVSVRRKKNSGTVPVYLNVYDLTPMNVYGYWLGIGIYHSGLE-------------- 60

Query: 61  YSAIFDLKLRNLWDSDAVHGIEFAFGAHEYPSTGIFEGEPKQCDGFKFRESILIGKTDMS 120
                            VHG+E+ +GAHE  S+GIFE EPK+C GF FR+SIL+G+T+M 
Sbjct: 61  -----------------VHGVEYGYGAHEKSSSGIFEVEPKKCPGFTFRKSILVGETEMK 120

Query: 121 EAEVRSLMEELGKDYRGNAYNLITKNCNHFCNHVCIKLTGNPIPNWVNRLARIGWICNCV 180
             EVRS ME+L ++Y+GN Y+LIT+NCNHFCNHV +KLT   IP+WVNRLAR+G++CNCV
Sbjct: 121 AKEVRSFMEKLSEEYQGNKYHLITRNCNHFCNHVSLKLTHKSIPSWVNRLARLGFLCNCV 180

Query: 181 LPATLNSTKFGQNRAQKKRGEEKQEEEEEEEKKKKELARRIQTAEISASNSSSSSSSSSS 240
           LPA LN TK       K+ G++ +   E E  KKK+  ++I+ +     +SSSS++   +
Sbjct: 181 LPACLNETKV------KRVGKDGKLLLEGENTKKKKRKKKIRRSRSGPLSSSSSNARLDN 225

Query: 241 TTTLRRGRSRTRRPRLPASPLIP 262
           T T  R  S T  P L ASP  P
Sbjct: 241 TPTHNRSIS-TGNPPLSASPSCP 225

[Close]

BLAST of Carg06534 vs. TAIR 10
Match: AT2G25190.1 (PPPDE putative thiol peptidase family protein )

HSP 1 Score: 203.8 bits (517), Expect = 1.8e-52
Identity = 120/253 (47.43%), Postives = 149/253 (58.89%), Query Frame = 0


Query: 12  SGSVPVYLNVYDLTHINGYAYWLGLGVYHSGVQENEGKLNDVWSRKIYSAIFDLKLRNLW 71
           SGSVPVYLNVYDLT +N Y YWLGLGV+HSGV+                           
Sbjct: 14  SGSVPVYLNVYDLTPMNAYGYWLGLGVFHSGVE--------------------------- 73

Query: 72  DSDAVHGIEFAFGAHEYPSTGIFEGEPKQCDGFKFRESILIGKTDMSEAEVRSLMEELGK 131
               VHG+E+AFGAHE  STGIFE EPK+C GF FR+SIL+GKTD+   EVR  ME+L +
Sbjct: 74  ----VHGVEYAFGAHESSSTGIFEVEPKKCPGFTFRKSILVGKTDLVAKEVRVFMEKLAE 133

Query: 132 DYRGNAYNLITKNCNHFCNHVCIKLTGNPIPNWVNRLARIGWICNCVLPATLNSTKFGQN 191
           +Y+GN Y+LIT+NCNHFCN VC+KL    IP WVNRLAR+G +CNCVLP  LN  K    
Sbjct: 134 EYQGNKYHLITRNCNHFCNEVCLKLAQKSIPRWVNRLARLGVLCNCVLPPRLNEAKV--- 193

Query: 192 RAQKKRGEEKQEEEEEEEKKKKELARRIQTAEISASNSS-----------SSSSSSSSTT 251
                R   K E  E E+KK +  +R       S S+S+           SS +  SS++
Sbjct: 194 -----RRVGKGELSESEKKKLRNRSRSDPLLSSSPSSSTPDNHRSHIRAKSSGNHPSSSS 227

Query: 252 TLRRGRSRTRRPR 254
           +   G  + RRP+
Sbjct: 254 SSSSGSKKNRRPK 227

[Close]

BLAST of Carg06534 vs. TAIR 10
Match: AT5G47310.1 (PPPDE putative thiol peptidase family protein )

HSP 1 Score: 185.7 bits (470), Expect = 5.0e-47
Identity = 84/197 (42.64%), Postives = 115/197 (58.38%), Query Frame = 0


Query: 9   NGDSGSVPVYLNVYDLTHINGYAYWLGLGVYHSGVQENEGKLNDVWSRKIYSAIFDLKLR 68
           NG+    PVYLNVYDLT +N Y YW GLG++HSG++                        
Sbjct: 22  NGEGSLTPVYLNVYDLTPVNNYLYWFGLGIFHSGIE------------------------ 81

Query: 69  NLWDSDAVHGIEFAFGAHEYPSTGIFEGEPKQCDGFKFRESILIGKTDMSEAEVRSLMEE 128
                   HG E+ +GAHEY S+G+FE EP+ C GF FR S+L+G T MS ++ RS ME+
Sbjct: 82  -------AHGFEYGYGAHEYSSSGVFEVEPRSCPGFIFRRSVLLGTTSMSRSDFRSFMEK 141

Query: 129 LGKDYRGNAYNLITKNCNHFCNHVCIKLTGNPIPNWVNRLARIGWICNCVLPATLNSTKF 188
           L + Y G+ Y+LI KNCNHF   VC+++TG PIP W+NR+AR+G  CNC+LP ++  +  
Sbjct: 142 LSRKYHGDTYHLIAKNCNHFTEEVCLQVTGKPIPGWINRMARVGSFCNCILPESIQLSSV 187

Query: 189 GQNRAQKKRGEEKQEEE 206
               A +   +    EE
Sbjct: 202 NHPEALEFSDDNDGSEE 187

The following BLAST results are available for this feature:

BLAST of Carg06534 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7018383.1	6.8e-147	100.00	DeSI-like protein [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6581964.1	5.4e-120	87.55	DeSI-like protein, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023528854.1	3.0e-118	87.40	deSI-like protein At4g17486 [Cucurbita pepo subsp. pepo] >XP_023528855.1 deSI-li...	[more]
XP_022955511.1	5.6e-117	86.04	deSI-like protein At4g17486 [Cucurbita moschata]	[more]
XP_022980803.1	4.7e-116	86.04	deSI-like protein At4g17486 [Cucurbita maxima]	[more]

BLAST of Carg06534 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93VG8	1.6e-45	42.44	DeSI-like protein At4g17486 OS=Arabidopsis thaliana OX=3702 GN=At4g17486 PE=2 SV...	[more]
Q6DC39	8.6e-28	35.86	Deubiquitinase DESI2 OS=Danio rerio OX=7955 GN=desi2 PE=2 SV=1	[more]
Q5PQ09	1.2e-26	35.74	Deubiquitinase DESI2 OS=Xenopus laevis OX=8355 GN=desi2 PE=2 SV=1	[more]
Q9BSY9	8.1e-26	36.18	Deubiquitinase DESI2 OS=Homo sapiens OX=9606 GN=DESI2 PE=1 SV=1	[more]
Q5XIT6	8.1e-26	36.18	Deubiquitinase DESI2 OS=Rattus norvegicus OX=10116 GN=Desi2 PE=2 SV=1	[more]

BLAST of Carg06534 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GTY9	2.7e-117	86.04	deSI-like protein At4g17486 OS=Cucurbita moschata OX=3662 GN=LOC111457516 PE=3 S...	[more]
A0A6J1J0C0	2.3e-116	86.04	deSI-like protein At4g17486 OS=Cucurbita maxima OX=3661 GN=LOC111480086 PE=3 SV=...	[more]
A0A0A0KX01	1.5e-104	77.07	PPPDE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G006490 PE=3 ...	[more]
A0A1S3BXH1	5.1e-100	75.19	deSI-like protein At4g17486 OS=Cucumis melo OX=3656 GN=LOC103494655 PE=3 SV=1	[more]
A0A6J1DIV3	9.7e-99	76.23	deSI-like protein At4g17486 OS=Momordica charantia OX=3673 GN=LOC111020936 PE=3 ...	[more]

BLAST of Carg06534 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G80690.1	1.8e-73	57.30	PPPDE putative thiol peptidase family protein	[more]
AT5G25170.1	1.8e-57	49.05	PPPDE putative thiol peptidase family protein	[more]
AT4G31980.1	9.1e-57	47.53	unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF247,...	[more]
AT2G25190.1	1.8e-52	47.43	PPPDE putative thiol peptidase family protein	[more]
AT5G47310.1	5.0e-47	42.64	PPPDE putative thiol peptidase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 190..225
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 192..219
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 190..265
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 221..243
None	No IPR available	PANTHER	PTHR12378:SF52	F23A5.4 PROTEIN	coord: 5..265
IPR008580	PPPDE peptidase domain	SMART	SM01179	DUF862_2a	coord: 15..184 e-value: 3.6E-50 score: 182.7
IPR008580	PPPDE peptidase domain	PFAM	PF05903	Peptidase_C97	coord: 16..180 e-value: 6.1E-44 score: 149.5
IPR008580	PPPDE peptidase domain	PANTHER	PTHR12378	DESUMOYLATING ISOPEPTIDASE	coord: 5..265
IPR008580	PPPDE peptidase domain	PROSITE	PS51858	PPPDE	coord: 15..183 score: 51.563778
IPR042266	PPPDE peptidase domain superfamily	GENE3D	3.90.1720.30	PPPDE domains	coord: 11..202 e-value: 6.6E-37 score: 128.5

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg06534-RA	Carg06534-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016579	protein deubiquitination
molecular_function	GO:0101005	deubiquitinase activity
molecular_function	GO:0008233	peptidase activity