Carg06396 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg06396
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Pectinesterase
Location	Carg_Chr14: 9490617 .. 9493761 (-)
RNA-Seq Expression	Carg06396
Synteny	Carg06396

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ACAAAGACATCCTCAATCTCCTCCAACCAATGGCACAAACCGACGACTCCACCGTCGCCGGAGCACCCATCACCGGCTGCCCCACTTCCATCTCCAACAAAAAGCACAATCTCTTCATTATAGTCCTCTCAGCCGCGCTTCTCCTCGCCGGCATCTCATCGGAAGACCACGTGACCGCCGTGAGAAGCACGTGCGCGGTCACTCTGTATCCCGAGCTATGCCAGTCGACAATATCCACCGCCGTGGAAACCAGCTCGAAGAAGGTGTCGAGTTCAAAAGACATAATCGAAGCGTCGGTAAACATCACGATTTCTGCAGTGAAACAAAACTATAAAAGCATCGAGAAATTGCTTGAAACGACGAAGCATCTGACAAAACGAGAGAAAATTGCGGTCCATGACTGTCTGGAAACAGGGGAGGAGACGCTTAGAGAGCTGTACGAGGTAGTGGAAGATCTCAATGAGTATCCCAAAAAGAAATCTCTGCCCCAATACGCCGATGACCTCAAAACCTTACTCAGTTCCGCCATTACTAATCAGGAGACATGCGTGGACGGATTTTCACACGACAGAGCCGACAAGAAGGTACGAGAATCATTGCTAGCAGGACAAATTCATGTCGAGAAATTGTGCAGTGTTGCCTTAGCCTTGATTAAGAATCTCACCGACACCGACATTGCCAATCATTACAACATCCGAGGAAGGTCCATGGCCATGTCGGAATTTAATTGGCCGGATTGGATGTCGACCGGTGACCGGAAGCTTTTGCAAGCGTCGTCTACGGCGACTCCAGATGTTGTAGTGGCAGCTGATGGAAGTGGCGATTTCCAGACAGTGTCAGCGGCTGTTGCGGCGGCGCCGAGTAGGAGTAGCCGGAGATATATAATAAGAATTAAAGCGGGGGTTTATAGGGAAAATGTGAATGTGCCGAGTAGTAAGACGAATATAATGTTTTGGGGAGATGGAAGAACCACCACCATAATCACCGGGAACAGGAATGTCGTCGACGGCAGCACCACCTTCAACTCCGCCACCGTTGGTATCACATCTTACTTTTCTAAATTCTCAATCTTTTATTATTTAATGCTGTCTTTATTTTTGGACCTAAAAAATAGTTTTCAGAACATTTAAAAAATAAAAAGAAAAATATTAAACTTTATTTTAATTTTTATATAATTTATATGGTACGAAATTTAATTTGTGGATATATATATATATATATATATATATATCTATATATATATATATTTTTGGTATGAAAAATCACCTAAATCTTATTTTTGTCCATAACTCGAAATTAATGTCAAGCTTTGACATTTACGCACAAAATATATATATATATATATATATATATATATATATATATATATATATATATATATTTGAAATTGGAAGTGAAACCTATGTGGCATAGGATCCCGCAAGTTGCTTCCATTGTTGCCTCATATATATATATATATATAATTATTTTAATTATAATTTATCCTACCTTTTCCTCCGCAAATAAGAAAAATACAAAAGAAATTAATTGTAAATTATAAATACGACATATTTTTAAATTAACCAAATAGGGGATGATAATTCTTATTTTCTACATCTTAAGTTCGAGTCTACTCTGAAGACTCGAATTGAGTGGAGAATCTCTTTTTTAAAGGGAATGAAGTAGATAATGGAAAGTGATTTGTTTCGGTAGGATAAATAGAAACTGAGATAGAGATTATATTTTCATCGTTCTGTCCCATTCCCGAACATGTCCCACTCTTCCCTATTTTTTTTAGTTATAAAATAAATAAATAAATATATTCTATTACAAATTATAATAAATCTTTTATTATTATTATTATTATTATTTTGGTACTATTACATCATTATTTTCTACCAAATAAGTTGTCATATTATTTAGGCCAATTATATTTTATTTTAACTAATAAAGGTAAATTTTTGGTCAAAATGAATTTTGGAAATTTTTATAAATATTATTATTTAAATATTCTCGAATTTTTTAGATTTTGATGGCAATATTTAAGTTAAAATTATATTGATTTTTTTAAATAAAAATTATGTTGTAATATTAAATTTAATTTTTTTAAAAAATACGTTGATAAAACATTATATTATATTTTTTATTTGTGAACCTTATATTAATTTTTTTTTGCATCCAATTTGTTAGATCTTTAACTAATTCTAAATTGTTGAAAATTAATTAAATTTGAATTTAATTTTTATATCTAATATAACTGTAAATTTTAATAAATTTGAAATATCAACCACTCTATAATTTTAAAAATTAATTAAAATAAAGAGTAAAGAGTAAATTAGAAATTAAGAACATCTCCAAAAGTTAATTTCCGATGATTTGCCTGTTTCTCTGTTGCAGCGGCGGTGGGAGGAAGGTTCTTGGCGAGAGATATTACGTTCCAGAACACCGCCGGTCCATCGAAGCACCAAGCGGTGGCTCTCCGCGTCGGCTCCGACCTCTCAGCCTTTTACCGCTGCGACATGCTCGCTTATCAAGACACGCTTTACGTCCACTCCAACCGTCAATTCTACGTCAGCTGCATCATCGTCGGCACCGTTGACTTCATCTTCGGCAATGCGGCTGCCGTCATTCAAAACTCCGACATCCACGCCCGCCGCCCCAATCCCGGCCAGAAGAATATGGTCACTGCACAGGGAAGAACCGACCCAAATCAGAACACCGGGATCGTCATCCAGAAATGCCGAATCGGAACCACCTCCGATTTGCAGCCGGTGATCAGAAATTTCCCCACGTTTCTGGGGCGGCCGTGGCAGAGGTACTCGAGGACGGTGGTTATGCAGACGAGCATTAGCAATGTGATTGATCCGGCGGGATGGCACATTTGGGATGGGAATTTTGCGCTTGATACTCTGTTTTATGCAGAGTATCAGAATAGTGGGGCCGGTGCTGATACCTCCAGAAGAGTGACGTGGAAGGGGTACAGGGTTATTAGCAGTGCGGCGGAAGCTGAGAGCTTCACGGCTGGGAAGTTCATCAGCGGCGGCACGTGGCTGAGCTCCACCGGATTCCCATTTTCACTTGGACTGTAGAATTAATAAGCTTCGGACTTTGTGAGTAATGACCAGTGGCTGTATTGTTGGTGTTCTGTTGCATCATCATTAATAAA

mRNA sequence

ACAAAGACATCCTCAATCTCCTCCAACCAATGGCACAAACCGACGACTCCACCGTCGCCGGAGCACCCATCACCGGCTGCCCCACTTCCATCTCCAACAAAAAGCACAATCTCTTCATTATAGTCCTCTCAGCCGCGCTTCTCCTCGCCGGCATCTCATCGGAAGACCACGTGACCGCCGTGAGAAGCACGTGCGCGGTCACTCTGTATCCCGAGCTATGCCAGTCGACAATATCCACCGCCGTGGAAACCAGCTCGAAGAAGGTGTCGAGTTCAAAAGACATAATCGAAGCGTCGGTAAACATCACGATTTCTGCAGTGAAACAAAACTATAAAAGCATCGAGAAATTGCTTGAAACGACGAAGCATCTGACAAAACGAGAGAAAATTGCGGTCCATGACTGTCTGGAAACAGGGGAGGAGACGCTTAGAGAGCTGTACGAGGTAGTGGAAGATCTCAATGAGTATCCCAAAAAGAAATCTCTGCCCCAATACGCCGATGACCTCAAAACCTTACTCAGTTCCGCCATTACTAATCAGGAGACATGCGTGGACGGATTTTCACACGACAGAGCCGACAAGAAGGTACGAGAATCATTGCTAGCAGGACAAATTCATGTCGAGAAATTGTGCAGTGTTGCCTTAGCCTTGATTAAGAATCTCACCGACACCGACATTGCCAATCATTACAACATCCGAGGAAGGTCCATGGCCATGTCGGAATTTAATTGGCCGGATTGGATGTCGACCGGTGACCGGAAGCTTTTGCAAGCGTCGTCTACGGCGACTCCAGATGTTGTAGTGGCAGCTGATGGAAGTGGCGATTTCCAGACAGTGTCAGCGGCTGTTGCGGCGGCGCCGAGTAGGAGTAGCCGGAGATATATAATAAGAATTAAAGCGGGGGTTTATAGGGAAAATGTGAATGTGCCGAGTAGTAAGACGAATATAATGTTTTGGGGAGATGGAAGAACCACCACCATAATCACCGGGAACAGGAATGTCGTCGACGGCAGCACCACCTTCAACTCCGCCACCGTTGCGGCGGTGGGAGGAAGGTTCTTGGCGAGAGATATTACGTTCCAGAACACCGCCGGTCCATCGAAGCACCAAGCGGTGGCTCTCCGCGTCGGCTCCGACCTCTCAGCCTTTTACCGCTGCGACATGCTCGCTTATCAAGACACGCTTTACGTCCACTCCAACCGTCAATTCTACGTCAGCTGCATCATCGTCGGCACCGTTGACTTCATCTTCGGCAATGCGGCTGCCGTCATTCAAAACTCCGACATCCACGCCCGCCGCCCCAATCCCGGCCAGAAGAATATGGTCACTGCACAGGGAAGAACCGACCCAAATCAGAACACCGGGATCGTCATCCAGAAATGCCGAATCGGAACCACCTCCGATTTGCAGCCGGTGATCAGAAATTTCCCCACGTTTCTGGGGCGGCCGTGGCAGAGGTACTCGAGGACGGTGGTTATGCAGACGAGCATTAGCAATGTGATTGATCCGGCGGGATGGCACATTTGGGATGGGAATTTTGCGCTTGATACTCTGTTTTATGCAGAGTATCAGAATAGTGGGGCCGGTGCTGATACCTCCAGAAGAGTGACGTGGAAGGGGTACAGGGTTATTAGCAGTGCGGCGGAAGCTGAGAGCTTCACGGCTGGGAAGTTCATCAGCGGCGGCACGTGGCTGAGCTCCACCGGATTCCCATTTTCACTTGGACTGTAGAATTAATAAGCTTCGGACTTTGTGAGTAATGACCAGTGGCTGTATTGTTGGTGTTCTGTTGCATCATCATTAATAAA

Coding sequence (CDS)

ATGGCACAAACCGACGACTCCACCGTCGCCGGAGCACCCATCACCGGCTGCCCCACTTCCATCTCCAACAAAAAGCACAATCTCTTCATTATAGTCCTCTCAGCCGCGCTTCTCCTCGCCGGCATCTCATCGGAAGACCACGTGACCGCCGTGAGAAGCACGTGCGCGGTCACTCTGTATCCCGAGCTATGCCAGTCGACAATATCCACCGCCGTGGAAACCAGCTCGAAGAAGGTGTCGAGTTCAAAAGACATAATCGAAGCGTCGGTAAACATCACGATTTCTGCAGTGAAACAAAACTATAAAAGCATCGAGAAATTGCTTGAAACGACGAAGCATCTGACAAAACGAGAGAAAATTGCGGTCCATGACTGTCTGGAAACAGGGGAGGAGACGCTTAGAGAGCTGTACGAGGTAGTGGAAGATCTCAATGAGTATCCCAAAAAGAAATCTCTGCCCCAATACGCCGATGACCTCAAAACCTTACTCAGTTCCGCCATTACTAATCAGGAGACATGCGTGGACGGATTTTCACACGACAGAGCCGACAAGAAGGTACGAGAATCATTGCTAGCAGGACAAATTCATGTCGAGAAATTGTGCAGTGTTGCCTTAGCCTTGATTAAGAATCTCACCGACACCGACATTGCCAATCATTACAACATCCGAGGAAGGTCCATGGCCATGTCGGAATTTAATTGGCCGGATTGGATGTCGACCGGTGACCGGAAGCTTTTGCAAGCGTCGTCTACGGCGACTCCAGATGTTGTAGTGGCAGCTGATGGAAGTGGCGATTTCCAGACAGTGTCAGCGGCTGTTGCGGCGGCGCCGAGTAGGAGTAGCCGGAGATATATAATAAGAATTAAAGCGGGGGTTTATAGGGAAAATGTGAATGTGCCGAGTAGTAAGACGAATATAATGTTTTGGGGAGATGGAAGAACCACCACCATAATCACCGGGAACAGGAATGTCGTCGACGGCAGCACCACCTTCAACTCCGCCACCGTTGCGGCGGTGGGAGGAAGGTTCTTGGCGAGAGATATTACGTTCCAGAACACCGCCGGTCCATCGAAGCACCAAGCGGTGGCTCTCCGCGTCGGCTCCGACCTCTCAGCCTTTTACCGCTGCGACATGCTCGCTTATCAAGACACGCTTTACGTCCACTCCAACCGTCAATTCTACGTCAGCTGCATCATCGTCGGCACCGTTGACTTCATCTTCGGCAATGCGGCTGCCGTCATTCAAAACTCCGACATCCACGCCCGCCGCCCCAATCCCGGCCAGAAGAATATGGTCACTGCACAGGGAAGAACCGACCCAAATCAGAACACCGGGATCGTCATCCAGAAATGCCGAATCGGAACCACCTCCGATTTGCAGCCGGTGATCAGAAATTTCCCCACGTTTCTGGGGCGGCCGTGGCAGAGGTACTCGAGGACGGTGGTTATGCAGACGAGCATTAGCAATGTGATTGATCCGGCGGGATGGCACATTTGGGATGGGAATTTTGCGCTTGATACTCTGTTTTATGCAGAGTATCAGAATAGTGGGGCCGGTGCTGATACCTCCAGAAGAGTGACGTGGAAGGGGTACAGGGTTATTAGCAGTGCGGCGGAAGCTGAGAGCTTCACGGCTGGGAAGTTCATCAGCGGCGGCACGTGGCTGAGCTCCACCGGATTCCCATTTTCACTTGGACTGTAG

Protein sequence

MAQTDDSTVAGAPITGCPTSISNKKHNLFIIVLSAALLLAGISSEDHVTAVRSTCAVTLYPELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIEKLLETTKHLTKREKIAVHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSHDRADKKVRESLLAGQIHVEKLCSVALALIKNLTDTDIANHYNIRGRSMAMSEFNWPDWMSTGDRKLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAGVYRENVNVPSSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGGRFLARDITFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAAAVIQNSDIHARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQRYSRTVVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQNSGAGADTSRRVTWKGYRVISSAAEAESFTAGKFISGGTWLSSTGFPFSLGL

Homology

[Close]

BLAST of Carg06396 vs. NCBI nr
Match: KAG7018245.1 (Pectinesterase/pectinesterase inhibitor U1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1105.1 bits (2857), Expect = 0.0e+00
Identity = 566/566 (100.00%), Postives = 566/566 (100.00%), Query Frame = 0


Query: 1   MAQTDDSTVAGAPITGCPTSISNKKHNLFIIVLSAALLLAGISSEDHVTAVRSTCAVTLY 60
           MAQTDDSTVAGAPITGCPTSISNKKHNLFIIVLSAALLLAGISSEDHVTAVRSTCAVTLY
Sbjct: 1   MAQTDDSTVAGAPITGCPTSISNKKHNLFIIVLSAALLLAGISSEDHVTAVRSTCAVTLY 60

Query: 61  PELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIEKLLETTKHLTKREKI 120
           PELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIEKLLETTKHLTKREKI
Sbjct: 61  PELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIEKLLETTKHLTKREKI 120

Query: 121 AVHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSHD 180
           AVHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSHD
Sbjct: 121 AVHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSHD 180

Query: 181 RADKKVRESLLAGQIHVEKLCSVALALIKNLTDTDIANHYNIRGRSMAMSEFNWPDWMST 240
           RADKKVRESLLAGQIHVEKLCSVALALIKNLTDTDIANHYNIRGRSMAMSEFNWPDWMST
Sbjct: 181 RADKKVRESLLAGQIHVEKLCSVALALIKNLTDTDIANHYNIRGRSMAMSEFNWPDWMST 240

Query: 241 GDRKLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAGVYRENVNVP 300
           GDRKLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAGVYRENVNVP
Sbjct: 241 GDRKLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAGVYRENVNVP 300

Query: 301 SSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGGRFLARDITFQNTAGPSKHQ 360
           SSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGGRFLARDITFQNTAGPSKHQ
Sbjct: 301 SSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGGRFLARDITFQNTAGPSKHQ 360

Query: 361 AVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAAAVIQNSDIH 420
           AVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAAAVIQNSDIH
Sbjct: 361 AVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAAAVIQNSDIH 420

Query: 421 ARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQRYSRT 480
           ARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQRYSRT
Sbjct: 421 ARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQRYSRT 480

Query: 481 VVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQNSGAGADTSRRVTWKGYRVISSAAEA 540
           VVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQNSGAGADTSRRVTWKGYRVISSAAEA
Sbjct: 481 VVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQNSGAGADTSRRVTWKGYRVISSAAEA 540

Query: 541 ESFTAGKFISGGTWLSSTGFPFSLGL 567
           ESFTAGKFISGGTWLSSTGFPFSLGL
Sbjct: 541 ESFTAGKFISGGTWLSSTGFPFSLGL 566

[Close]

BLAST of Carg06396 vs. NCBI nr
Match: KAG6581794.1 (Pectinesterase/pectinesterase inhibitor U1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1099.7 bits (2843), Expect = 0.0e+00
Identity = 562/566 (99.29%), Postives = 565/566 (99.82%), Query Frame = 0


Query: 1   MAQTDDSTVAGAPITGCPTSISNKKHNLFIIVLSAALLLAGISSEDHVTAVRSTCAVTLY 60
           MAQTDDS VAGAPITGCPTSISNKKHNLFIIVLSAALLLAG+SSEDHVTAVRSTCAVTLY
Sbjct: 1   MAQTDDSPVAGAPITGCPTSISNKKHNLFIIVLSAALLLAGVSSEDHVTAVRSTCAVTLY 60

Query: 61  PELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIEKLLETTKHLTKREKI 120
           PELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSI+KLLETTKHLTKREKI
Sbjct: 61  PELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIKKLLETTKHLTKREKI 120

Query: 121 AVHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSHD 180
           A+HDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSHD
Sbjct: 121 ALHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSHD 180

Query: 181 RADKKVRESLLAGQIHVEKLCSVALALIKNLTDTDIANHYNIRGRSMAMSEFNWPDWMST 240
           RADKKVRESLLAGQIHVEKLCSVALALIKNLTDTDIANHYNIRGRSMAMSEFNWPDWMST
Sbjct: 181 RADKKVRESLLAGQIHVEKLCSVALALIKNLTDTDIANHYNIRGRSMAMSEFNWPDWMST 240

Query: 241 GDRKLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAGVYRENVNVP 300
           GDRKLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAGVYRENVNVP
Sbjct: 241 GDRKLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAGVYRENVNVP 300

Query: 301 SSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGGRFLARDITFQNTAGPSKHQ 360
           SSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGGRFLARDITFQNTAGPSKHQ
Sbjct: 301 SSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGGRFLARDITFQNTAGPSKHQ 360

Query: 361 AVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAAAVIQNSDIH 420
           AVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAAAVIQNSDIH
Sbjct: 361 AVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAAAVIQNSDIH 420

Query: 421 ARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQRYSRT 480
           ARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQRYSRT
Sbjct: 421 ARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQRYSRT 480

Query: 481 VVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQNSGAGADTSRRVTWKGYRVISSAAEA 540
           VVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQNSGAGADTSRRVTWKGYRVISSAAEA
Sbjct: 481 VVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQNSGAGADTSRRVTWKGYRVISSAAEA 540

Query: 541 ESFTAGKFISGGTWLSSTGFPFSLGL 567
           ESFTAGKFISGGTWLSSTGFPFSLGL
Sbjct: 541 ESFTAGKFISGGTWLSSTGFPFSLGL 566

[Close]

BLAST of Carg06396 vs. NCBI nr
Match: XP_022955430.1 (pectinesterase-like [Cucurbita moschata])

HSP 1 Score: 1095.1 bits (2831), Expect = 0.0e+00
Identity = 558/566 (98.59%), Postives = 563/566 (99.47%), Query Frame = 0


Query: 1   MAQTDDSTVAGAPITGCPTSISNKKHNLFIIVLSAALLLAGISSEDHVTAVRSTCAVTLY 60
           MAQTDDSTVAGAPITGCPTSISNKKHNLFIIVLSAALLLAG+SSEDHVTAVRSTCAVTLY
Sbjct: 1   MAQTDDSTVAGAPITGCPTSISNKKHNLFIIVLSAALLLAGVSSEDHVTAVRSTCAVTLY 60

Query: 61  PELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIEKLLETTKHLTKREKI 120
           PELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSI+KLLETTKHLTKREKI
Sbjct: 61  PELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIKKLLETTKHLTKREKI 120

Query: 121 AVHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSHD 180
           A+HDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSHD
Sbjct: 121 ALHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSHD 180

Query: 181 RADKKVRESLLAGQIHVEKLCSVALALIKNLTDTDIANHYNIRGRSMAMSEFNWPDWMST 240
           RADKKVRESLLAGQ HVEKLCSVALALIKNLTDTDIANHYNIRGRSMAMSEFNWPDWMS 
Sbjct: 181 RADKKVRESLLAGQFHVEKLCSVALALIKNLTDTDIANHYNIRGRSMAMSEFNWPDWMSP 240

Query: 241 GDRKLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAGVYRENVNVP 300
           GDRKLLQASSTATPD VVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAGVYRENVNVP
Sbjct: 241 GDRKLLQASSTATPDAVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAGVYRENVNVP 300

Query: 301 SSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGGRFLARDITFQNTAGPSKHQ 360
           SSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGGRFLARDITFQNTAGPSKHQ
Sbjct: 301 SSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGGRFLARDITFQNTAGPSKHQ 360

Query: 361 AVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAAAVIQNSDIH 420
           AVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAAAVIQNSDIH
Sbjct: 361 AVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAAAVIQNSDIH 420

Query: 421 ARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQRYSRT 480
           ARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQRYSRT
Sbjct: 421 ARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQRYSRT 480

Query: 481 VVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQNSGAGADTSRRVTWKGYRVISSAAEA 540
           VVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQN+GAGADTSRRVTWKGYR+ISSAAEA
Sbjct: 481 VVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQNNGAGADTSRRVTWKGYRIISSAAEA 540

Query: 541 ESFTAGKFISGGTWLSSTGFPFSLGL 567
           ESFTAGKFISGGTWLSSTGFPFSLGL
Sbjct: 541 ESFTAGKFISGGTWLSSTGFPFSLGL 566

[Close]

BLAST of Carg06396 vs. NCBI nr
Match: XP_023526589.1 (pectinesterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1086.2 bits (2808), Expect = 0.0e+00
Identity = 553/566 (97.70%), Postives = 562/566 (99.29%), Query Frame = 0


Query: 1   MAQTDDSTVAGAPITGCPTSISNKKHNLFIIVLSAALLLAGISSEDHVTAVRSTCAVTLY 60
           MAQTDDSTVAGAP TG PTSISNKKHNLFI+ LSAA+LLAG+SS+DHVTAVRSTCAVTLY
Sbjct: 1   MAQTDDSTVAGAPSTGSPTSISNKKHNLFILALSAAVLLAGVSSDDHVTAVRSTCAVTLY 60

Query: 61  PELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIEKLLETTKHLTKREKI 120
           PELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSI+KLLETTKHLTKREKI
Sbjct: 61  PELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIKKLLETTKHLTKREKI 120

Query: 121 AVHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSHD 180
           A+HDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSHD
Sbjct: 121 ALHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSHD 180

Query: 181 RADKKVRESLLAGQIHVEKLCSVALALIKNLTDTDIANHYNIRGRSMAMSEFNWPDWMST 240
           RADKK+RESLLAGQIHVEKLCSVALALIKNLTDTDIANHYNIRGRSMAMSEFNWPDWMS 
Sbjct: 181 RADKKIRESLLAGQIHVEKLCSVALALIKNLTDTDIANHYNIRGRSMAMSEFNWPDWMSP 240

Query: 241 GDRKLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAGVYRENVNVP 300
           GDR+LLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAGVYRENVNVP
Sbjct: 241 GDRRLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAGVYRENVNVP 300

Query: 301 SSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGGRFLARDITFQNTAGPSKHQ 360
           SSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGGRFLARDITFQNTAGPSKHQ
Sbjct: 301 SSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGGRFLARDITFQNTAGPSKHQ 360

Query: 361 AVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAAAVIQNSDIH 420
           AVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAAAVIQNSDIH
Sbjct: 361 AVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAAAVIQNSDIH 420

Query: 421 ARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQRYSRT 480
           ARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQRYSRT
Sbjct: 421 ARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQRYSRT 480

Query: 481 VVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQNSGAGADTSRRVTWKGYRVISSAAEA 540
           VVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQNSGAGADTSRRVTWKGYRVISSAA+A
Sbjct: 481 VVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQNSGAGADTSRRVTWKGYRVISSAADA 540

Query: 541 ESFTAGKFISGGTWLSSTGFPFSLGL 567
           ESFTAGKFISGGTWLSSTGFPFSLGL
Sbjct: 541 ESFTAGKFISGGTWLSSTGFPFSLGL 566

[Close]

BLAST of Carg06396 vs. NCBI nr
Match: XP_022980581.1 (pectinesterase-like [Cucurbita maxima])

HSP 1 Score: 1065.4 bits (2754), Expect = 1.6e-307
Identity = 547/571 (95.80%), Postives = 557/571 (97.55%), Query Frame = 0


Query: 1   MAQTDDSTVAGAPITGCPTSISNKKHNLFIIVLSAALLLAGISSEDHVTAVRSTCAVTLY 60
           MAQTDDSTVAGAPIT  PTSIS KKHNLFI+ LSAALLL G+SS+DHV AVRSTCAVTLY
Sbjct: 1   MAQTDDSTVAGAPITSSPTSISKKKHNLFILALSAALLLDGVSSDDHVAAVRSTCAVTLY 60

Query: 61  PELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIEKLLETTKHLTKREKI 120
           PELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSI+KL+ETTK+LTKREKI
Sbjct: 61  PELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIKKLVETTKNLTKREKI 120

Query: 121 AVHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSHD 180
           A+HDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSHD
Sbjct: 121 ALHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSHD 180

Query: 181 RADKKVRESLLAGQIHVEKLCSVALALIKNLTDTDIANHY-----NIRGRSMAMSEFNWP 240
           RADKK+RESLLAGQIHVEKLCSVALALIKNLTDTDIANHY     NI+GRSMAMSEFNWP
Sbjct: 181 RADKKIRESLLAGQIHVEKLCSVALALIKNLTDTDIANHYKLTGNNIQGRSMAMSEFNWP 240

Query: 241 DWMSTGDRKLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAGVYRE 300
           DWMS GDRKLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAGVYRE
Sbjct: 241 DWMSPGDRKLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAGVYRE 300

Query: 301 NVNVPSSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGGRFLARDITFQNTAG 360
           NVNVPSSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVG RFLARDITFQNTAG
Sbjct: 301 NVNVPSSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDITFQNTAG 360

Query: 361 PSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAAAVIQ 420
           PSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAAAVIQ
Sbjct: 361 PSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAAAVIQ 420

Query: 421 NSDIHARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQ 480
           NSDIHARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQ
Sbjct: 421 NSDIHARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQ 480

Query: 481 RYSRTVVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQNSGAGADTSRRVTWKGYRVIS 540
           RYSRTVVMQTSISNVIDPAGWHIWDGNFALDTLFYAE+QNSGAGADTSRRVTWKGYRVIS
Sbjct: 481 RYSRTVVMQTSISNVIDPAGWHIWDGNFALDTLFYAEFQNSGAGADTSRRVTWKGYRVIS 540

Query: 541 SAAEAESFTAGKFISGGTWLSSTGFPFSLGL 567
           SAAEAE FTAGKFISGGTWLSSTGFPFSLGL
Sbjct: 541 SAAEAEGFTAGKFISGGTWLSSTGFPFSLGL 571

[Close]

BLAST of Carg06396 vs. ExPASy Swiss-Prot
Match: Q43143 (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PMEU1 PE=2 SV=1)

HSP 1 Score: 731.5 bits (1887), Expect = 7.3e-210
Identity = 372/569 (65.38%), Postives = 453/569 (79.61%), Query Frame = 0


Query: 24  KKHNLFIIVLSAALLLAGI------------SSEDHV----------TAVRSTCAVTLYP 83
           +K  +++ ++++ LL+A +            +S+DH             V+S C+ TL+P
Sbjct: 17  RKKKIYLAIVASVLLVAAVIGVVAGVKSHSKNSDDHADIMAISSSAHAIVKSACSNTLHP 76

Query: 84  ELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIEKLLETTKHLTKREKIA 143
           ELC S I   V   SKKV+S KD+IE S+NIT+ AV++NY ++++L++T K LT REK+A
Sbjct: 77  ELCYSAI-VNVSDFSKKVTSQKDVIELSLNITVKAVRRNYYAVKELIKTRKGLTPREKVA 136

Query: 144 VHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSHDR 203
           +HDCLET +ETL EL+  VEDL  YP KKSL ++ +DLKTL+SSAITNQETC+DGFSHD 
Sbjct: 137 LHDCLETMDETLDELHTAVEDLELYPNKKSLKEHVEDLKTLISSAITNQETCLDGFSHDE 196

Query: 204 ADKKVRESLLAGQIHVEKLCSVALALIKNLTDTDIANHYNI----RGRSMAMSEFNWPDW 263
           ADKKVR+ LL GQ HVEK+CS ALA+I N+TDTDIAN   +      R +      WP+W
Sbjct: 197 ADKKVRKVLLKGQKHVEKMCSNALAMICNMTDTDIANEMKLSAPANNRKLVEDNGEWPEW 256

Query: 264 MSTGDRKLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAGVYRENV 323
           +S GDR+LLQ SST TPDVVVAADGSGD++TVS AV  AP +SS+RY+IRIKAGVYRENV
Sbjct: 257 LSAGDRRLLQ-SSTVTPDVVVAADGSGDYKTVSEAVRKAPEKSSKRYVIRIKAGVYRENV 316

Query: 324 NVPSSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGGRFLARDITFQNTAGPS 383
           +VP  KTNIMF GDG++ TIIT +RNV DGSTTF+SATV  V G+ LARDITFQNTAG S
Sbjct: 317 DVPKKKTNIMFMGDGKSNTIITASRNVQDGSTTFHSATVVRVAGKVLARDITFQNTAGAS 376

Query: 384 KHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAAAVIQNS 443
           KHQAVAL VGSDLSAFYRCDMLAYQDTLYVHSNRQF+V C++ GTVDFIFGN AAV Q+ 
Sbjct: 377 KHQAVALCVGSDLSAFYRCDMLAYQDTLYVHSNRQFFVQCLVAGTVDFIFGNGAAVFQDC 436

Query: 444 DIHARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQRY 503
           DIHARRP  GQKNMVTAQGRTDPNQNTGIVIQKCRIG TSDL+PV ++FPT+LGRPW+ Y
Sbjct: 437 DIHARRPGSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLRPVQKSFPTYLGRPWKEY 496

Query: 504 SRTVVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQNSGAGADTSRRVTWKGYRVISSA 563
           SRTV+MQ+SI++VI PAGWH W+GNFALDTLFY EY N+GAGA TS RV WKG++VI+S+
Sbjct: 497 SRTVIMQSSITDVIQPAGWHEWNGNFALDTLFYGEYANTGAGAPTSGRVKWKGHKVITSS 556

Query: 564 AEAESFTAGKFISGGTWLSSTGFPFSLGL 567
            EA+++T G+FI+GG+WLSSTGFPFSLGL
Sbjct: 557 TEAQAYTPGRFIAGGSWLSSTGFPFSLGL 583

[Close]

BLAST of Carg06396 vs. ExPASy Swiss-Prot
Match: P83948 (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)

HSP 1 Score: 723.8 bits (1867), Expect = 1.5e-207
Identity = 376/567 (66.31%), Postives = 454/567 (80.07%), Query Frame = 0


Query: 19  TSISNKKHNLFIIVLSAALLLAGI----------------SSEDHVTAVRSTCAVTLYPE 78
           ++I  KK  LF+ + +  L++A +                 +E H   ++S+C+ T YP+
Sbjct: 21  SNIPKKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSSCSSTRYPD 80

Query: 79  LCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIEKLLETTKHLTKREKIAV 138
           LC S I+ AV  +SKKV+S KD+IE S+NIT +AV+ NY  I+KLL+ T +LTKREK+A+
Sbjct: 81  LCFSAIA-AVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRT-NLTKREKVAL 140

Query: 139 HDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSHDRA 198
           HDCLET +ETL EL++ VEDL EYP KKSL Q+ADDLKTL+S+A+TNQ TC+DGFSHD A
Sbjct: 141 HDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDA 200

Query: 199 DKKVRESLLAGQIHVEKLCSVALALIKNLTDTDIANHYNIRGRSM---AMSEFNWPDWMS 258
           +K VR++L  GQ+HVEK+CS ALA+IKN+TDTD+        R +     +   WP W+S
Sbjct: 201 NKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNNRKLIEETSTVDGWPAWLS 260

Query: 259 TGDRKLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAGVYRENVNV 318
           TGDR+LLQ SS+ TP+VVVAADGSG+F+TV+A+VAAAP   ++RYIIRIKAGVYRENV V
Sbjct: 261 TGDRRLLQ-SSSVTPNVVVAADGSGNFKTVAASVAAAPQGGTKRYIIRIKAGVYRENVEV 320

Query: 319 PSSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGGRFLARDITFQNTAGPSKH 378
                NIMF GDGRT TIITG+RNVVDGSTTF SATVA VG  FLARDITFQNTAGPSKH
Sbjct: 321 TKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVAVVGEGFLARDITFQNTAGPSKH 380

Query: 379 QAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAAAVIQNSDI 438
           QAVALRVG+DLSAFY CDMLAYQDTLYVHSNRQF+V+C+I GTVDFIFGNAAAV+QN DI
Sbjct: 381 QAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAVLQNCDI 440

Query: 439 HARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQRYSR 498
           HAR+PN GQKNMVTAQGR DPNQNTGIVIQK RIG TSDL+PV  +FPT+LGRPW+ YSR
Sbjct: 441 HARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSR 500

Query: 499 TVVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQNSGAGADTSRRVTWKGYRVISSAAE 558
           TV+MQ+SI++VI PAGWH WDGNFAL+TLFY E+QN+GAGA TS RV WKG+RVI+SA E
Sbjct: 501 TVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTSGRVKWKGFRVITSATE 560

Query: 559 AESFTAGKFISGGTWLSSTGFPFSLGL 567
           A++FT G FI+G +WL STGFPFSLGL
Sbjct: 561 AQAFTPGSFIAGSSWLGSTGFPFSLGL 584

[Close]

BLAST of Carg06396 vs. ExPASy Swiss-Prot
Match: O04886 (Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 721.5 bits (1861), Expect = 7.6e-207
Identity = 375/567 (66.14%), Postives = 452/567 (79.72%), Query Frame = 0


Query: 19  TSISNKKHNLFIIVLSAALLLAGI----------------SSEDHVTAVRSTCAVTLYPE 78
           ++I  KK  LF+ + +  L++A +                 +E H   ++S+C+ T YP+
Sbjct: 21  SNIPKKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSSCSSTRYPD 80

Query: 79  LCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIEKLLETTKHLTKREKIAV 138
           LC S I+ AV  +SKKV+S KD+IE S+NIT +AV+ NY  I+KLL+ T +LTKREK+A+
Sbjct: 81  LCFSAIA-AVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRT-NLTKREKVAL 140

Query: 139 HDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSHDRA 198
           HDCLET +ETL EL++ VEDL EYP KKSL Q+ADDLKTL+S+A+TNQ TC+DGFSHD A
Sbjct: 141 HDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDA 200

Query: 199 DKKVRESLLAGQIHVEKLCSVALALIKNLTDTDIANHYNIRGRSM---AMSEFNWPDWMS 258
           +K VR++L  GQ+HVEK+CS ALA+IKN+TDTD+        R +     +   WP W+S
Sbjct: 201 NKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNNRKLTEETSTVDGWPAWLS 260

Query: 259 TGDRKLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAGVYRENVNV 318
            GDR+LLQ SS+ TP+ VVAADGSG+F+TV+AAVAAAP   ++RYIIRIKAGVYRENV V
Sbjct: 261 PGDRRLLQ-SSSVTPNAVVAADGSGNFKTVAAAVAAAPQGGTKRYIIRIKAGVYRENVEV 320

Query: 319 PSSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGGRFLARDITFQNTAGPSKH 378
                NIMF GDGRT TIITG+RNVVDGSTTF SAT A VG  FLARDITFQNTAGPSKH
Sbjct: 321 TKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATAAVVGEGFLARDITFQNTAGPSKH 380

Query: 379 QAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAAAVIQNSDI 438
           QAVALRVG+DLSAFY CDMLAYQDTLYVHSNRQF+V+C+I GTVDFIFGNAAAV+QN DI
Sbjct: 381 QAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAVLQNCDI 440

Query: 439 HARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQRYSR 498
           HAR+PN GQKNMVTAQGRTDPNQNTGIVIQK RIG TSDL+PV  +FPT+LGRPW+ YSR
Sbjct: 441 HARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSR 500

Query: 499 TVVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQNSGAGADTSRRVTWKGYRVISSAAE 558
           TV+MQ+SI+++I PAGWH WDGNFAL+TLFY E+QNSGAGA TS RV WKG+RVI+SA E
Sbjct: 501 TVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKGFRVITSATE 560

Query: 559 AESFTAGKFISGGTWLSSTGFPFSLGL 567
           A++FT G FI+G +WL STGFPFSLGL
Sbjct: 561 AQAFTPGSFIAGSSWLGSTGFPFSLGL 584

[Close]

BLAST of Carg06396 vs. ExPASy Swiss-Prot
Match: O49006 (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME3 PE=1 SV=2)

HSP 1 Score: 702.2 bits (1811), Expect = 4.8e-201
Identity = 376/587 (64.05%), Postives = 444/587 (75.64%), Query Frame = 0


Query: 20  SISNKKHNLFIIVLSAALLL------AGIS------------SEDHVTAVRSTCAVTLYP 79
           S  N K N  +++LSAA+ L      AGIS            S      +RS+C+ T YP
Sbjct: 10  SKDNFKKNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSHAVLRSSCSSTRYP 69

Query: 80  ELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIEKLLETTKHLTKREKIA 139
           ELC   IS  V     +++S KD+IEASVN+TI+AV+ NY +++KL++  K LT REK A
Sbjct: 70  ELC---ISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTA 129

Query: 140 VHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSHDR 199
           +HDCLET +ETL EL+E VEDL+ YP KK+L ++A DLKTL+SSAITNQETC+DGFSHD 
Sbjct: 130 LHDCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDD 189

Query: 200 ADKKVRESLLAGQIHVEKLCSVALALIKNLTDTDIAN---HYNIRGRSMAMSEFN----- 259
           ADK+VR++LL GQIHVE +CS ALA+IKN+TDTDIAN      I   +  + E N     
Sbjct: 190 ADKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTV 249

Query: 260 --------------WPDWMSTGDRKLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSR 319
                         WP W+S GDR+LLQ S     D  VAADGSG F+TV+AAVAAAP  
Sbjct: 250 AVDIAGAGELDSEGWPTWLSAGDRRLLQGSGVKA-DATVAADGSGTFKTVAAAVAAAPEN 309

Query: 320 SSRRYIIRIKAGVYRENVNVPSSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAV 379
           S++RY+I IKAGVYRENV V   K NIMF GDGRT TIITG+RNVVDGSTTF+SATVAAV
Sbjct: 310 SNKRYVIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVAAV 369

Query: 380 GGRFLARDITFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCII 439
           G RFLARDITFQNTAGPSKHQAVALRVGSD SAFY CDMLAYQDTLYVHSNRQF+V C+I
Sbjct: 370 GERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKCLI 429

Query: 440 VGTVDFIFGNAAAVIQNSDIHARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDL 499
            GTVDFIFGNAA V+Q+ DIHARRPN GQKNMVTAQGRTDPNQNTGIVIQKCRIG TSDL
Sbjct: 430 AGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDL 489

Query: 500 QPVIRNFPTFLGRPWQRYSRTVVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQNSGAG 559
           Q V  +FPT+LGRPW+ YS+TV+MQ++IS+VI P GW  W G FAL+TL Y EY N+GAG
Sbjct: 490 QSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTGAG 549

Query: 560 ADTSRRVTWKGYRVISSAAEAESFTAGKFISGGTWLSSTGFPFSLGL 567
           A T+ RV W+G++VI++AAEA+ +TAG+FI GG WLSSTGFPFSLGL
Sbjct: 550 AGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

[Close]

BLAST of Carg06396 vs. ExPASy Swiss-Prot
Match: Q42534 (Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2)

HSP 1 Score: 690.3 bits (1780), Expect = 1.9e-197
Identity = 372/592 (62.84%), Postives = 444/592 (75.00%), Query Frame = 0


Query: 12  APITGCPTSISNKKHNLFIIVLSAA---LLLAGI-------------------SSEDHVT 71
           API    +  S+ K+N  +I+ SAA   LLLA I                   SS  H  
Sbjct: 2   APIKEFISKFSDFKNNKKLILSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSH-A 61

Query: 72  AVRSTCAVTLYPELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIEKLLE 131
            ++S C+ TLYPELC S ++    T  K+++S K++IEAS+N+T  AVK NY +++KL+ 
Sbjct: 62  ILKSVCSSTLYPELCFSAVAA---TGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIA 121

Query: 132 TTKHLTKREKIAVHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITN 191
             K LT RE  A+HDCLET +ETL EL+  VEDL++YPK+KSL ++ADDLKTL+SSAITN
Sbjct: 122 KRKGLTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITN 181

Query: 192 QETCVDGFSHDRADKKVRESLLAGQIHVEKLCSVALALIKNLTDTDIANHYNIRGRSMAM 251
           Q TC+DGFS+D AD+KVR++LL GQ+HVE +CS ALA+IKN+T+TDIAN + +R +S   
Sbjct: 182 QGTCLDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIAN-FELRDKSSTF 241

Query: 252 SEFN---------------WPDWMSTGDRKLLQASSTATPDVVVAADGSGDFQTVSAAVA 311
           +  N               WP W+S GDR+LLQ  ST   D  VA DGSGDF TV+AAVA
Sbjct: 242 TNNNNRKLKEVTGDLDSDGWPKWLSVGDRRLLQ-GSTIKADATVADDGSGDFTTVAAAVA 301

Query: 312 AAPSRSSRRYIIRIKAGVYRENVNVPSSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSA 371
           AAP +S++R++I IKAGVYRENV V   KTNIMF GDGR  TIITG+RNVVDGSTTF+SA
Sbjct: 302 AAPEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSA 361

Query: 372 TVAAVGGRFLARDITFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFY 431
           TVAAVG RFLARDITFQNTAGPSKHQAVALRVGSD SAFY+CDM AYQDTLYVHSNRQF+
Sbjct: 362 TVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFF 421

Query: 432 VSCIIVGTVDFIFGNAAAVIQNSDIHARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIG 491
           V C I GTVDFIFGNAAAV+Q+ DI+ARRPN GQKNMVTAQGR+DPNQNTGIVIQ CRIG
Sbjct: 422 VKCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIG 481

Query: 492 TTSDLQPVIRNFPTFLGRPWQRYSRTVVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQ 551
            TSDL  V   FPT+LGRPW+ YSRTV+MQ+ IS+VI P GWH W G+FALDTL Y EY 
Sbjct: 482 GTSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYL 541

Query: 552 NSGAGADTSRRVTWKGYRVISSAAEAESFTAGKFISGGTWLSSTGFPFSLGL 567
           N G GA T+ RV WKGY+VI+S  EA+ FTAG+FI GG WL+STGFPFSL L
Sbjct: 542 NRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

[Close]

BLAST of Carg06396 vs. ExPASy TrEMBL
Match: A0A6J1GV42 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111457458 PE=3 SV=1)

HSP 1 Score: 1095.1 bits (2831), Expect = 0.0e+00
Identity = 558/566 (98.59%), Postives = 563/566 (99.47%), Query Frame = 0


Query: 1   MAQTDDSTVAGAPITGCPTSISNKKHNLFIIVLSAALLLAGISSEDHVTAVRSTCAVTLY 60
           MAQTDDSTVAGAPITGCPTSISNKKHNLFIIVLSAALLLAG+SSEDHVTAVRSTCAVTLY
Sbjct: 1   MAQTDDSTVAGAPITGCPTSISNKKHNLFIIVLSAALLLAGVSSEDHVTAVRSTCAVTLY 60

Query: 61  PELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIEKLLETTKHLTKREKI 120
           PELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSI+KLLETTKHLTKREKI
Sbjct: 61  PELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIKKLLETTKHLTKREKI 120

Query: 121 AVHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSHD 180
           A+HDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSHD
Sbjct: 121 ALHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSHD 180

Query: 181 RADKKVRESLLAGQIHVEKLCSVALALIKNLTDTDIANHYNIRGRSMAMSEFNWPDWMST 240
           RADKKVRESLLAGQ HVEKLCSVALALIKNLTDTDIANHYNIRGRSMAMSEFNWPDWMS 
Sbjct: 181 RADKKVRESLLAGQFHVEKLCSVALALIKNLTDTDIANHYNIRGRSMAMSEFNWPDWMSP 240

Query: 241 GDRKLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAGVYRENVNVP 300
           GDRKLLQASSTATPD VVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAGVYRENVNVP
Sbjct: 241 GDRKLLQASSTATPDAVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAGVYRENVNVP 300

Query: 301 SSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGGRFLARDITFQNTAGPSKHQ 360
           SSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGGRFLARDITFQNTAGPSKHQ
Sbjct: 301 SSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGGRFLARDITFQNTAGPSKHQ 360

Query: 361 AVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAAAVIQNSDIH 420
           AVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAAAVIQNSDIH
Sbjct: 361 AVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAAAVIQNSDIH 420

Query: 421 ARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQRYSRT 480
           ARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQRYSRT
Sbjct: 421 ARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQRYSRT 480

Query: 481 VVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQNSGAGADTSRRVTWKGYRVISSAAEA 540
           VVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQN+GAGADTSRRVTWKGYR+ISSAAEA
Sbjct: 481 VVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQNNGAGADTSRRVTWKGYRIISSAAEA 540

Query: 541 ESFTAGKFISGGTWLSSTGFPFSLGL 567
           ESFTAGKFISGGTWLSSTGFPFSLGL
Sbjct: 541 ESFTAGKFISGGTWLSSTGFPFSLGL 566

[Close]

BLAST of Carg06396 vs. ExPASy TrEMBL
Match: A0A6J1ITZ4 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111479907 PE=3 SV=1)

HSP 1 Score: 1065.4 bits (2754), Expect = 7.9e-308
Identity = 547/571 (95.80%), Postives = 557/571 (97.55%), Query Frame = 0


Query: 1   MAQTDDSTVAGAPITGCPTSISNKKHNLFIIVLSAALLLAGISSEDHVTAVRSTCAVTLY 60
           MAQTDDSTVAGAPIT  PTSIS KKHNLFI+ LSAALLL G+SS+DHV AVRSTCAVTLY
Sbjct: 1   MAQTDDSTVAGAPITSSPTSISKKKHNLFILALSAALLLDGVSSDDHVAAVRSTCAVTLY 60

Query: 61  PELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIEKLLETTKHLTKREKI 120
           PELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSI+KL+ETTK+LTKREKI
Sbjct: 61  PELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIKKLVETTKNLTKREKI 120

Query: 121 AVHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSHD 180
           A+HDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSHD
Sbjct: 121 ALHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSHD 180

Query: 181 RADKKVRESLLAGQIHVEKLCSVALALIKNLTDTDIANHY-----NIRGRSMAMSEFNWP 240
           RADKK+RESLLAGQIHVEKLCSVALALIKNLTDTDIANHY     NI+GRSMAMSEFNWP
Sbjct: 181 RADKKIRESLLAGQIHVEKLCSVALALIKNLTDTDIANHYKLTGNNIQGRSMAMSEFNWP 240

Query: 241 DWMSTGDRKLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAGVYRE 300
           DWMS GDRKLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAGVYRE
Sbjct: 241 DWMSPGDRKLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAGVYRE 300

Query: 301 NVNVPSSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGGRFLARDITFQNTAG 360
           NVNVPSSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVG RFLARDITFQNTAG
Sbjct: 301 NVNVPSSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDITFQNTAG 360

Query: 361 PSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAAAVIQ 420
           PSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAAAVIQ
Sbjct: 361 PSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAAAVIQ 420

Query: 421 NSDIHARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQ 480
           NSDIHARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQ
Sbjct: 421 NSDIHARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQ 480

Query: 481 RYSRTVVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQNSGAGADTSRRVTWKGYRVIS 540
           RYSRTVVMQTSISNVIDPAGWHIWDGNFALDTLFYAE+QNSGAGADTSRRVTWKGYRVIS
Sbjct: 481 RYSRTVVMQTSISNVIDPAGWHIWDGNFALDTLFYAEFQNSGAGADTSRRVTWKGYRVIS 540

Query: 541 SAAEAESFTAGKFISGGTWLSSTGFPFSLGL 567
           SAAEAE FTAGKFISGGTWLSSTGFPFSLGL
Sbjct: 541 SAAEAEGFTAGKFISGGTWLSSTGFPFSLGL 571

[Close]

BLAST of Carg06396 vs. ExPASy TrEMBL
Match: A0A0A0LCR6 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G646640 PE=3 SV=1)

HSP 1 Score: 889.4 bits (2297), Expect = 7.8e-255
Identity = 462/576 (80.21%), Postives = 497/576 (86.28%), Query Frame = 0


Query: 1   MAQTDDSTVAGAPITGCPTSISNKKHNLFIIVLSAALLLAGISSEDHVTAVRSTCAVTLY 60
           M++ DD+  AG P +G        K NL +  LS  LLLA +SS+DH   V S CA+TLY
Sbjct: 1   MSKPDDTPAAGVPFSG------YSKTNLLVFALSTTLLLAVVSSDDH---VGSKCAMTLY 60

Query: 61  PELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIEKLLETTKHLTKREKI 120
           PELC++TISTAV       SSSK+ IEASVNITISAVK NYK ++KLL+T K+LTKR+KI
Sbjct: 61  PELCETTISTAVG------SSSKEAIEASVNITISAVKDNYKRVQKLLKTVKNLTKRQKI 120

Query: 121 AVHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSHD 180
           A HDCLETGEETLRELYEVVED+NEYPKKKSL +YADDLKTLLSS ITNQETCVDGFSHD
Sbjct: 121 AFHDCLETGEETLRELYEVVEDVNEYPKKKSLSRYADDLKTLLSSTITNQETCVDGFSHD 180

Query: 181 RADKKVRESLLAGQIHVEKLCSVALALIKNLTDTDIA--------NHYNIRGRSMAMSE- 240
           + DKKVRESL  G IH+EKLCS+ALALIKNLTDTDIA        NH N +       E 
Sbjct: 181 KGDKKVRESLKEGLIHIEKLCSIALALIKNLTDTDIANLNNNNNNNHLNRKQLEEKKMED 240

Query: 241 -FNWPDWMSTGDRKLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKA 300
              WPDWMS  DR+LLQASSTATPDVVVAADGSGDF+T+S AVAAAPSRSSRRYIIRIKA
Sbjct: 241 GIKWPDWMSPKDRRLLQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSRRYIIRIKA 300

Query: 301 GVYRENVNVPSSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGGRFLARDITF 360
           GVYRENVNV SSK NIMFWGDGR  TIITGNRNVVDGSTTFNSATVAAVG RFLARD+TF
Sbjct: 301 GVYRENVNVASSKRNIMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTF 360

Query: 361 QNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNA 420
           QNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYV CIIVGT+DFIFGNA
Sbjct: 361 QNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNA 420

Query: 421 AAVIQNSDIHARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFL 480
           AAVIQ+ DIHARRPNPGQ+NMVTAQGRTDPNQNTGIVIQKCRIGTTSDL+PVI NFPTFL
Sbjct: 421 AAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFL 480

Query: 481 GRPWQRYSRTVVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQNSGAGADTSRRVTWKG 540
           GRPWQRYSRTVVMQTSISNVIDPAGWH+WDGNFALDTLFYAEYQNSGAGADTSRRV WKG
Sbjct: 481 GRPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVKWKG 540

Query: 541 YRVISSAAEAESFTAGKFISGGTWLSSTGFPFSLGL 567
           +RV++ AAEAE+FTAG FI GGTWLSSTGFPFSLGL
Sbjct: 541 FRVLTRAAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 561

[Close]

BLAST of Carg06396 vs. ExPASy TrEMBL
Match: A0A5A7UEV0 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold522G001090 PE=3 SV=1)

HSP 1 Score: 885.9 bits (2288), Expect = 8.6e-254
Identity = 457/574 (79.62%), Postives = 494/574 (86.06%), Query Frame = 0


Query: 1   MAQTDDSTVAGAPITGCPTSISNKKHNLFIIVLSAALLLAGISSEDHVTAVRSTCAVTLY 60
           M++ D++  AG P +       N K NL +  LS  LLLA +SS+DH   VRS CA+TLY
Sbjct: 1   MSKPDETLAAGVPFS------CNSKTNLLVFALSTTLLLAAVSSDDH---VRSKCAMTLY 60

Query: 61  PELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIEKLLETTKHLTKREKI 120
           PELC++TISTAV       SSSK+ IEASVNITI AV  NY+ ++KLL+T   LTKREKI
Sbjct: 61  PELCETTISTAVG------SSSKEAIEASVNITIGAVGDNYRRVKKLLKTENSLTKREKI 120

Query: 121 AVHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSHD 180
           A+HDCLETGEETLRELYEVVED+NEYP KKSL +YADDLKTLLSSAITNQETC+DGFSHD
Sbjct: 121 ALHDCLETGEETLRELYEVVEDVNEYPNKKSLSRYADDLKTLLSSAITNQETCLDGFSHD 180

Query: 181 RADKKVRESLLAGQIHVEKLCSVALALIKNLTDTDIANHYNIRG--------RSMAMSEF 240
           +ADKKVRESL  G IH+EKLCS+ALALIKNLTDTDIAN  N                   
Sbjct: 181 KADKKVRESLKEGLIHIEKLCSIALALIKNLTDTDIANLNNNNNHLSRKQLKEKNTDDRI 240

Query: 241 NWPDWMSTGDRKLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAGV 300
           NWPDWMS  DR+LLQASSTATPDVVVAADGSGDF+T+S AVAAAPSRSS+RYIIRIKAGV
Sbjct: 241 NWPDWMSPNDRRLLQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSQRYIIRIKAGV 300

Query: 301 YRENVNVPSSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGGRFLARDITFQN 360
           YRENVNV SSK NIMFWGDGR  TIITGNRNVVDGSTTFNSATVAAVG RFLARD+TFQN
Sbjct: 301 YRENVNVASSKRNIMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQN 360

Query: 361 TAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAAA 420
           TAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYV CIIVGT+DFIFGNAAA
Sbjct: 361 TAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAA 420

Query: 421 VIQNSDIHARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGR 480
           VIQ+ DIHARRPNPGQ+NMVTAQGRTDPNQNTGIVIQKCRIGTTSDL+PVI NFPTFLGR
Sbjct: 421 VIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGR 480

Query: 481 PWQRYSRTVVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQNSGAGADTSRRVTWKGYR 540
           PWQRYSRTVVMQTSISNVIDPAGWH+WDGNFALDTLFYAEYQNSGAGADTSRRV WKG+R
Sbjct: 481 PWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVRWKGFR 540

Query: 541 VISSAAEAESFTAGKFISGGTWLSSTGFPFSLGL 567
           V++ AAEAE+FTAG FI GGTWLSSTGFPFSLGL
Sbjct: 541 VLTRAAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 559

[Close]

BLAST of Carg06396 vs. ExPASy TrEMBL
Match: A0A1S3CCA5 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499204 PE=3 SV=1)

HSP 1 Score: 885.9 bits (2288), Expect = 8.6e-254
Identity = 457/574 (79.62%), Postives = 494/574 (86.06%), Query Frame = 0


Query: 1   MAQTDDSTVAGAPITGCPTSISNKKHNLFIIVLSAALLLAGISSEDHVTAVRSTCAVTLY 60
           M++ D++  AG P +       N K NL +  LS  LLLA +SS+DH   VRS CA+TLY
Sbjct: 1   MSKPDETLAAGVPFS------CNSKTNLLVFALSTTLLLAAVSSDDH---VRSKCAMTLY 60

Query: 61  PELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIEKLLETTKHLTKREKI 120
           PELC++TISTAV       SSSK+ IEASVNITI AV  NY+ ++KLL+T   LTKREKI
Sbjct: 61  PELCETTISTAVG------SSSKEAIEASVNITIGAVGDNYRRVKKLLKTENSLTKREKI 120

Query: 121 AVHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSHD 180
           A+HDCLETGEETLRELYEVVED+NEYP KKSL +YADDLKTLLSSAITNQETC+DGFSHD
Sbjct: 121 ALHDCLETGEETLRELYEVVEDVNEYPNKKSLSRYADDLKTLLSSAITNQETCLDGFSHD 180

Query: 181 RADKKVRESLLAGQIHVEKLCSVALALIKNLTDTDIANHYNIRG--------RSMAMSEF 240
           +ADKKVRESL  G IH+EKLCS+ALALIKNLTDTDIAN  N                   
Sbjct: 181 KADKKVRESLKEGLIHIEKLCSIALALIKNLTDTDIANLNNNNNHLSRKQLKEKNTDDRI 240

Query: 241 NWPDWMSTGDRKLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAGV 300
           NWPDWMS  DR+LLQASSTATPDVVVAADGSGDF+T+S AVAAAPSRSS+RYIIRIKAGV
Sbjct: 241 NWPDWMSPNDRRLLQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSQRYIIRIKAGV 300

Query: 301 YRENVNVPSSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGGRFLARDITFQN 360
           YRENVNV SSK NIMFWGDGR  TIITGNRNVVDGSTTFNSATVAAVG RFLARD+TFQN
Sbjct: 301 YRENVNVASSKRNIMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQN 360

Query: 361 TAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAAA 420
           TAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYV CIIVGT+DFIFGNAAA
Sbjct: 361 TAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAA 420

Query: 421 VIQNSDIHARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGR 480
           VIQ+ DIHARRPNPGQ+NMVTAQGRTDPNQNTGIVIQKCRIGTTSDL+PVI NFPTFLGR
Sbjct: 421 VIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGR 480

Query: 481 PWQRYSRTVVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQNSGAGADTSRRVTWKGYR 540
           PWQRYSRTVVMQTSISNVIDPAGWH+WDGNFALDTLFYAEYQNSGAGADTSRRV WKG+R
Sbjct: 481 PWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVRWKGFR 540

Query: 541 VISSAAEAESFTAGKFISGGTWLSSTGFPFSLGL 567
           V++ AAEAE+FTAG FI GGTWLSSTGFPFSLGL
Sbjct: 541 VLTRAAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 559

[Close]

BLAST of Carg06396 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 702.2 bits (1811), Expect = 3.4e-202
Identity = 376/587 (64.05%), Postives = 444/587 (75.64%), Query Frame = 0


Query: 20  SISNKKHNLFIIVLSAALLL------AGIS------------SEDHVTAVRSTCAVTLYP 79
           S  N K N  +++LSAA+ L      AGIS            S      +RS+C+ T YP
Sbjct: 10  SKDNFKKNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSHAVLRSSCSSTRYP 69

Query: 80  ELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIEKLLETTKHLTKREKIA 139
           ELC   IS  V     +++S KD+IEASVN+TI+AV+ NY +++KL++  K LT REK A
Sbjct: 70  ELC---ISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTA 129

Query: 140 VHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSHDR 199
           +HDCLET +ETL EL+E VEDL+ YP KK+L ++A DLKTL+SSAITNQETC+DGFSHD 
Sbjct: 130 LHDCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDD 189

Query: 200 ADKKVRESLLAGQIHVEKLCSVALALIKNLTDTDIAN---HYNIRGRSMAMSEFN----- 259
           ADK+VR++LL GQIHVE +CS ALA+IKN+TDTDIAN      I   +  + E N     
Sbjct: 190 ADKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTV 249

Query: 260 --------------WPDWMSTGDRKLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSR 319
                         WP W+S GDR+LLQ S     D  VAADGSG F+TV+AAVAAAP  
Sbjct: 250 AVDIAGAGELDSEGWPTWLSAGDRRLLQGSGVKA-DATVAADGSGTFKTVAAAVAAAPEN 309

Query: 320 SSRRYIIRIKAGVYRENVNVPSSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAV 379
           S++RY+I IKAGVYRENV V   K NIMF GDGRT TIITG+RNVVDGSTTF+SATVAAV
Sbjct: 310 SNKRYVIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVAAV 369

Query: 380 GGRFLARDITFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCII 439
           G RFLARDITFQNTAGPSKHQAVALRVGSD SAFY CDMLAYQDTLYVHSNRQF+V C+I
Sbjct: 370 GERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKCLI 429

Query: 440 VGTVDFIFGNAAAVIQNSDIHARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDL 499
            GTVDFIFGNAA V+Q+ DIHARRPN GQKNMVTAQGRTDPNQNTGIVIQKCRIG TSDL
Sbjct: 430 AGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDL 489

Query: 500 QPVIRNFPTFLGRPWQRYSRTVVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQNSGAG 559
           Q V  +FPT+LGRPW+ YS+TV+MQ++IS+VI P GW  W G FAL+TL Y EY N+GAG
Sbjct: 490 QSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTGAG 549

Query: 560 ADTSRRVTWKGYRVISSAAEAESFTAGKFISGGTWLSSTGFPFSLGL 567
           A T+ RV W+G++VI++AAEA+ +TAG+FI GG WLSSTGFPFSLGL
Sbjct: 550 AGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

[Close]

BLAST of Carg06396 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 690.3 bits (1780), Expect = 1.3e-198
Identity = 372/592 (62.84%), Postives = 444/592 (75.00%), Query Frame = 0


Query: 12  APITGCPTSISNKKHNLFIIVLSAA---LLLAGI-------------------SSEDHVT 71
           API    +  S+ K+N  +I+ SAA   LLLA I                   SS  H  
Sbjct: 2   APIKEFISKFSDFKNNKKLILSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSH-A 61

Query: 72  AVRSTCAVTLYPELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIEKLLE 131
            ++S C+ TLYPELC S ++    T  K+++S K++IEAS+N+T  AVK NY +++KL+ 
Sbjct: 62  ILKSVCSSTLYPELCFSAVAA---TGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIA 121

Query: 132 TTKHLTKREKIAVHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITN 191
             K LT RE  A+HDCLET +ETL EL+  VEDL++YPK+KSL ++ADDLKTL+SSAITN
Sbjct: 122 KRKGLTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITN 181

Query: 192 QETCVDGFSHDRADKKVRESLLAGQIHVEKLCSVALALIKNLTDTDIANHYNIRGRSMAM 251
           Q TC+DGFS+D AD+KVR++LL GQ+HVE +CS ALA+IKN+T+TDIAN + +R +S   
Sbjct: 182 QGTCLDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIAN-FELRDKSSTF 241

Query: 252 SEFN---------------WPDWMSTGDRKLLQASSTATPDVVVAADGSGDFQTVSAAVA 311
           +  N               WP W+S GDR+LLQ  ST   D  VA DGSGDF TV+AAVA
Sbjct: 242 TNNNNRKLKEVTGDLDSDGWPKWLSVGDRRLLQ-GSTIKADATVADDGSGDFTTVAAAVA 301

Query: 312 AAPSRSSRRYIIRIKAGVYRENVNVPSSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSA 371
           AAP +S++R++I IKAGVYRENV V   KTNIMF GDGR  TIITG+RNVVDGSTTF+SA
Sbjct: 302 AAPEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSA 361

Query: 372 TVAAVGGRFLARDITFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFY 431
           TVAAVG RFLARDITFQNTAGPSKHQAVALRVGSD SAFY+CDM AYQDTLYVHSNRQF+
Sbjct: 362 TVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFF 421

Query: 432 VSCIIVGTVDFIFGNAAAVIQNSDIHARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIG 491
           V C I GTVDFIFGNAAAV+Q+ DI+ARRPN GQKNMVTAQGR+DPNQNTGIVIQ CRIG
Sbjct: 422 VKCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIG 481

Query: 492 TTSDLQPVIRNFPTFLGRPWQRYSRTVVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQ 551
            TSDL  V   FPT+LGRPW+ YSRTV+MQ+ IS+VI P GWH W G+FALDTL Y EY 
Sbjct: 482 GTSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYL 541

Query: 552 NSGAGADTSRRVTWKGYRVISSAAEAESFTAGKFISGGTWLSSTGFPFSLGL 567
           N G GA T+ RV WKGY+VI+S  EA+ FTAG+FI GG WL+STGFPFSL L
Sbjct: 542 NRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

[Close]

BLAST of Carg06396 vs. TAIR 10
Match: AT3G43270.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 405.6 bits (1041), Expect = 6.5e-113
Identity = 215/448 (47.99%), Postives = 280/448 (62.50%), Query Frame = 0


Query: 121 AVHDCLETGEETLRELYEVVE-DLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSH 180
           A+ DC++  +    EL  ++    +   K  S      DL+T +S+A++NQ+TC+DGF  
Sbjct: 80  AIVDCVDLLDSAAEELSWIISASQSPNGKDNSTGDVGSDLRTWISAALSNQDTCLDGF-- 139

Query: 181 DRADKKVRESLLAGQIHVEKLCSVALALIKNLTDTDIANHYNIRGRSMAMSEFN-WPDWM 240
           +  +  +++ +  G   V       L ++ +            +  + A S F+ +P W+
Sbjct: 140 EGTNGIIKKIVAGGLSKVGTTVRNLLTMVHSPPSKPKPKPIKAQTMTKAHSGFSKFPSWV 199

Query: 241 STGDRKLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAGVYRENVN 300
             GDRKLLQ  +    D VVAADG+G+F T+S AV AAP  S++RY+I +K GVY ENV 
Sbjct: 200 KPGDRKLLQTDNITVADAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVENVE 259

Query: 301 VPSSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGGRFLARDITFQNTAGPSK 360
           +   K NIM  GDG   T+ITGNR+ +DG TTF SAT A  G  F+ARDITFQNTAGP K
Sbjct: 260 IKKKKWNIMMVGDGIDATVITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNTAGPEK 319

Query: 361 HQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAAAVIQNSD 420
           HQAVA+R  +DL  FYRC M  YQDTLY HS RQF+  CII GTVDFIFG+A AV Q+  
Sbjct: 320 HQAVAIRSDTDLGVFYRCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQSCQ 379

Query: 421 IHARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQRYS 480
           I A++  P QKN +TAQGR DPN+ TG  IQ   I   +DL   +    T+LGRPW+ YS
Sbjct: 380 IKAKQGLPNQKNSITAQGRKDPNEPTGFTIQFSNIAADTDLLLNLNTTATYLGRPWKLYS 439

Query: 481 RTVVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQNSGAGADTSRRVTWKGYRVISSAA 540
           RTV MQ  +S+ I+P GW  W+GNFALDTL+Y EY NSG GA   RRV W GY V++++A
Sbjct: 440 RTVFMQNYMSDAINPVGWLEWNGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHVLNTSA 499

Query: 541 EAESFTAGKFISGGTWLSSTGFPFSLGL 567
           EA +FT  + I G  WL STG  F  GL
Sbjct: 500 EANNFTVSQLIQGNLWLPSTGITFIAGL 525

[Close]

BLAST of Carg06396 vs. TAIR 10
Match: AT1G11580.1 (methylesterase PCR A )

HSP 1 Score: 403.3 bits (1035), Expect = 3.2e-112
Identity = 243/567 (42.86%), Postives = 331/567 (58.38%), Query Frame = 0


Query: 13  PITGCPTSISNKKHNLFIIVLSAALLLAGI-------------SSEDHVTAVRSTCAVTL 72
           P+   P S+   KH    +VLS   +L  +             +++D +      C    
Sbjct: 7   PLLSKPKSL---KHKNLCLVLSFVAILGSVAFFTAQLISVNTNNNDDSLLTTSQICHGAH 66

Query: 73  YPELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIEKLLETTKHLTKREK 132
             + CQ+ +S     S  K+ +  D++   +  ++  ++     + +    +  +  R+K
Sbjct: 67  DQDSCQALLSEFTTLSLSKL-NRLDLLHVFLKNSVWRLESTMTMVSEARIRSNGV--RDK 126

Query: 133 IAVHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSH 192
               DC E  + +   +   +E+L       +L  Y+ ++ T LSS +TN  TC++  S 
Sbjct: 127 AGFADCEEMMDVSKDRMMSSMEELR--GGNYNLESYS-NVHTWLSSVLTNYMTCLESISD 186

Query: 193 DRADKKVRESLLAGQIH-VEKLCSVALALIKNLTDTDIANHYNIRGRSMAMSEFNWPDWM 252
              + K    ++  Q+  +     VALA+  ++           R     +    +P W+
Sbjct: 187 VSVNSK---QIVKPQLEDLVSRARVALAIFVSVLPA--------RDDLKMIISNRFPSWL 246

Query: 253 STGDRKLLQASS---TATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAGVYRE 312
           +  DRKLL++S      T +VVVA DG+G F+TV+ AVAAAP  S+ RY+I +K GVY+E
Sbjct: 247 TALDRKLLESSPKTLKVTANVVVAKDGTGKFKTVNEAVAAAPENSNTRYVIYVKKGVYKE 306

Query: 313 NVNVPSSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGGRFLARDITFQNTAG 372
            +++   K N+M  GDG+  TIITG+ NV+DGSTTF SATVAA G  F+A+DI FQNTAG
Sbjct: 307 TIDIGKKKKNLMLVGDGKDATIITGSLNVIDGSTTFRSATVAANGDGFMAQDIWFQNTAG 366

Query: 373 PSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAAAVIQ 432
           P+KHQAVALRV +D +   RC + AYQDTLY H+ RQFY    I GTVDFIFGN+A V Q
Sbjct: 367 PAKHQAVALRVSADQTVINRCRIDAYQDTLYTHTLRQFYRDSYITGTVDFIFGNSAVVFQ 426

Query: 433 NSDIHARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQ 492
           N DI AR P  GQKNM+TAQGR D NQNT I IQKC+I  +SDL PV  +  TFLGRPW+
Sbjct: 427 NCDIVARNPGAGQKNMLTAQGREDQNQNTAISIQKCKITASSDLAPVKGSVKTFLGRPWK 486

Query: 493 RYSRTVVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQNSGAGADTSRRVTWKGYRVIS 552
            YSRTV+MQ+ I N IDPAGW  WDG FAL TL+Y EY N+G GADTS+RV WKG++VI 
Sbjct: 487 LYSRTVIMQSFIDNHIDPAGWFPWDGEFALSTLYYGEYANTGPGADTSKRVNWKGFKVIK 546

Query: 553 SAAEAESFTAGKFISGGTWLSSTGFPF 563
            + EAE FT  K I GG WL  TG  F
Sbjct: 547 DSKEAEQFTVAKLIQGGLWLKPTGVTF 553

[Close]

BLAST of Carg06396 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 401.4 bits (1030), Expect = 1.2e-111
Identity = 245/550 (44.55%), Postives = 341/550 (62.00%), Query Frame = 0


Query: 27  NLFIIVLSAALLLAGISSEDHVTAVRSTCAVTLYPELCQSTISTAVETSSKKVSSSKDII 86
           N  I+ +  A  ++G + +D    V++ C+ T  P+ C+  ++    ++++ + S  + +
Sbjct: 10  NFVILCILVASTVSGYNQKD----VKAWCSQTPNPKPCEYFLTH--NSNNEPIKSESEFL 69

Query: 87  EASVNITISAVKQNYKSIEKLLETTKHLTKREKIAVHDCLETGEETLRELYEVVEDLNEY 146
           + S+ + +   +             K    REK A  DC++  + T+ ++ E ++   + 
Sbjct: 70  KISMKLVLD--RAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKC 129

Query: 147 PKKKSLPQYADDLKTLLSSAITNQETCVDGFSHDRADKKVRESLLAGQIHVEKLCSVALA 206
            K         D +T LS+A+TN +TC  GF            L  G      +  + L 
Sbjct: 130 SKL--------DAQTWLSTALTNLDTCRAGF------------LELG------VTDIVLP 189

Query: 207 LIKNLTDTDIANHYNIRGRSMAMSE--FNW--------PDWMSTGDRKLLQASSTATPDV 266
           L+ N       N  N+   ++A+++  FN+        P W+  GDRKLLQ SST   + 
Sbjct: 190 LMSN-------NVSNLLCNTLAINKVPFNYTPPEKDGFPSWVKPGDRKLLQ-SSTPKDNA 249

Query: 267 VVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAGVYRENVNVPSSKTNIMFWGDGRTTT 326
           VVA DGSG+F+T+  A+ AA    S R++I +K GVY EN+ +   K N+M  GDG   T
Sbjct: 250 VVAKDGSGNFKTIKEAIDAA--SGSGRFVIYVKQGVYSENLEI--RKKNVMLRGDGIGKT 309

Query: 327 IITGNRNVVDGSTTFNSATVAAVGGRFLARDITFQNTAGPSKHQAVALRVGSDLSAFYRC 386
           IITG+++V  G+TTFNSATVAAVG  F+AR ITF+NTAG S  QAVALR GSDLS FY+C
Sbjct: 310 IITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNTAGASNEQAVALRSGSDLSVFYQC 369

Query: 387 DMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAAAVIQNSDIHARRPNPGQKNMVTAQG 446
              AYQDTLYVHSNRQFY  C + GTVDFIFGNAAAV+QN +I ARRP   + N +TAQG
Sbjct: 370 SFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCNIFARRPR-SKTNTITAQG 429

Query: 447 RTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQRYSRTVVMQTSISNVIDPAGW 506
           R+DPNQNTGI+I   R+   SDL+PV+ +  T+LGRPW++YSRTV M+TS+ ++IDP GW
Sbjct: 430 RSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQYSRTVFMKTSLDSLIDPRGW 489

Query: 507 HIWDGNFALDTLFYAEYQNSGAGADTSRRVTWKGYRVISSAAEAESFTAGKFISGGTWLS 566
             WDGNFAL TLFYAE+QN+G GA TS RVTW G+RV+ SA+EA  FT G F++GG+W+ 
Sbjct: 490 LEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGSASEASKFTVGTFLAGGSWIP 511

The following BLAST results are available for this feature:

BLAST of Carg06396 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7018245.1	0.0e+00	100.00	Pectinesterase/pectinesterase inhibitor U1 [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6581794.1	0.0e+00	99.29	Pectinesterase/pectinesterase inhibitor U1, partial [Cucurbita argyrosperma subs...	[more]
XP_022955430.1	0.0e+00	98.59	pectinesterase-like [Cucurbita moschata]	[more]
XP_023526589.1	0.0e+00	97.70	pectinesterase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022980581.1	1.6e-307	95.80	pectinesterase-like [Cucurbita maxima]	[more]

BLAST of Carg06396 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q43143	7.3e-210	65.38	Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PM...	[more]
P83948	1.5e-207	66.31	Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1	[more]
O04886	7.6e-207	66.14	Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1	[more]
O49006	4.8e-201	64.05	Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME...	[more]
Q42534	1.9e-197	62.84	Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2	[more]

BLAST of Carg06396 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GV42	0.0e+00	98.59	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111457458 PE=3 SV=1	[more]
A0A6J1ITZ4	7.9e-308	95.80	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111479907 PE=3 SV=1	[more]
A0A0A0LCR6	7.8e-255	80.21	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G646640 PE=3 SV=1	[more]
A0A5A7UEV0	8.6e-254	79.62	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold522G00109...	[more]
A0A1S3CCA5	8.6e-254	79.62	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499204 PE=3 SV=1	[more]

BLAST of Carg06396 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G14310.1	3.4e-202	64.05	pectin methylesterase 3	[more]
AT1G53830.1	1.3e-198	62.84	pectin methylesterase 2	[more]
AT3G43270.1	6.5e-113	47.99	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT1G11580.1	3.2e-112	42.86	methylesterase PCR A	[more]
AT2G45220.1	1.2e-111	44.55	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 46..207 e-value: 8.8E-36 score: 134.9
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 32..212 e-value: 5.9E-21 score: 72.6
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 50..207 e-value: 2.6E-26 score: 92.8
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 48..213 e-value: 1.3E-29 score: 105.2
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 50..212
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 255..551 e-value: 2.1E-143 score: 476.5
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 249..566 e-value: 4.1E-124 score: 416.1
None	No IPR available	PANTHER	PTHR31707:SF180	PECTINESTERASE	coord: 46..565
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 46..565
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 53..212 e-value: 3.24275E-35 score: 127.94
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 399..408
IPR018040	Pectinesterase, Tyr active site	PROSITE	PS00800	PECTINESTERASE_1	coord: 280..299
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 249..565

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg06396-RA	Carg06396-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity