Carg06384 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg06384
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	17.4 kDa class III heat shock protein
Location	Carg_Chr14: 9425302 .. 9425778 (+)
RNA-Seq Expression	Carg06384
Synteny	Carg06384

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGCAGGGCCGCCGATTCGAACTCATCCAACGGACATTTGGTGCAGGCTATAAGCACCCTCTTCCCTTTCCCTGAAGCCCTCGAGAAATTAGCCTTCATTTCCGACGATGCTCGGGAGCCTAAGGGCATGTCCAGCATCCCGACTGACATCCTTGACGCTCCGAAGGAATACGTGTTCTATATGGACCTGCCGGGGCTGTCGAAATCCGACATTCAGGTGACGGTGGAGGAGGAGAACACGCTGGTGATTCGGAGTGGAGGGAAGAGGAAGAGGGAGGATGGGGAGGAGGAAGGGTGCAAGTATTTGAGGCTTGAGCGGAAGGCGCCGCAGAAGATGGTGAGGAAGTTCAGGCTGCCGGAGAATGCTGATACTTATGCCGTTTCTGCTAAGTGCGAGAGTGGGGTGCTGGTGGTGGTTGTGGGGAAGCTGCCTCCGCCTCCCAAGCCCAAAACGGTGCAGGTCGCCGTCGCCTGA

mRNA sequence

ATGAGCAGGGCCGCCGATTCGAACTCATCCAACGGACATTTGGTGCAGGCTATAAGCACCCTCTTCCCTTTCCCTGAAGCCCTCGAGAAATTAGCCTTCATTTCCGACGATGCTCGGGAGCCTAAGGGCATGTCCAGCATCCCGACTGACATCCTTGACGCTCCGAAGGAATACGTGTTCTATATGGACCTGCCGGGGCTGTCGAAATCCGACATTCAGGTGACGGTGGAGGAGGAGAACACGCTGGTGATTCGGAGTGGAGGGAAGAGGAAGAGGGAGGATGGGGAGGAGGAAGGGTGCAAGTATTTGAGGCTTGAGCGGAAGGCGCCGCAGAAGATGGTGAGGAAGTTCAGGCTGCCGGAGAATGCTGATACTTATGCCGTTTCTGCTAAGTGCGAGAGTGGGGTGCTGGTGGTGGTTGTGGGGAAGCTGCCTCCGCCTCCCAAGCCCAAAACGGTGCAGGTCGCCGTCGCCTGA

Coding sequence (CDS)

ATGAGCAGGGCCGCCGATTCGAACTCATCCAACGGACATTTGGTGCAGGCTATAAGCACCCTCTTCCCTTTCCCTGAAGCCCTCGAGAAATTAGCCTTCATTTCCGACGATGCTCGGGAGCCTAAGGGCATGTCCAGCATCCCGACTGACATCCTTGACGCTCCGAAGGAATACGTGTTCTATATGGACCTGCCGGGGCTGTCGAAATCCGACATTCAGGTGACGGTGGAGGAGGAGAACACGCTGGTGATTCGGAGTGGAGGGAAGAGGAAGAGGGAGGATGGGGAGGAGGAAGGGTGCAAGTATTTGAGGCTTGAGCGGAAGGCGCCGCAGAAGATGGTGAGGAAGTTCAGGCTGCCGGAGAATGCTGATACTTATGCCGTTTCTGCTAAGTGCGAGAGTGGGGTGCTGGTGGTGGTTGTGGGGAAGCTGCCTCCGCCTCCCAAGCCCAAAACGGTGCAGGTCGCCGTCGCCTGA

Protein sequence

MSRAADSNSSNGHLVQAISTLFPFPEALEKLAFISDDAREPKGMSSIPTDILDAPKEYVFYMDLPGLSKSDIQVTVEEENTLVIRSGGKRKREDGEEEGCKYLRLERKAPQKMVRKFRLPENADTYAVSAKCESGVLVVVVGKLPPPPKPKTVQVAVA

Homology

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BLAST of Carg06384 vs. NCBI nr
Match: KAG7018234.1 (17.4 kDa class III heat shock protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 308.5 bits (789), Expect = 3.3e-80
Identity = 158/158 (100.00%), Postives = 158/158 (100.00%), Query Frame = 0


Query: 1   MSRAADSNSSNGHLVQAISTLFPFPEALEKLAFISDDAREPKGMSSIPTDILDAPKEYVF 60
           MSRAADSNSSNGHLVQAISTLFPFPEALEKLAFISDDAREPKGMSSIPTDILDAPKEYVF
Sbjct: 1   MSRAADSNSSNGHLVQAISTLFPFPEALEKLAFISDDAREPKGMSSIPTDILDAPKEYVF 60

Query: 61  YMDLPGLSKSDIQVTVEEENTLVIRSGGKRKREDGEEEGCKYLRLERKAPQKMVRKFRLP 120
           YMDLPGLSKSDIQVTVEEENTLVIRSGGKRKREDGEEEGCKYLRLERKAPQKMVRKFRLP
Sbjct: 61  YMDLPGLSKSDIQVTVEEENTLVIRSGGKRKREDGEEEGCKYLRLERKAPQKMVRKFRLP 120

Query: 121 ENADTYAVSAKCESGVLVVVVGKLPPPPKPKTVQVAVA 159
           ENADTYAVSAKCESGVLVVVVGKLPPPPKPKTVQVAVA
Sbjct: 121 ENADTYAVSAKCESGVLVVVVGKLPPPPKPKTVQVAVA 158

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BLAST of Carg06384 vs. NCBI nr
Match: KAG6581781.1 (17.4 kDa class III heat shock protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 305.1 bits (780), Expect = 3.6e-79
Identity = 157/158 (99.37%), Postives = 157/158 (99.37%), Query Frame = 0


Query: 1   MSRAADSNSSNGHLVQAISTLFPFPEALEKLAFISDDAREPKGMSSIPTDILDAPKEYVF 60
           MSRAADSNSSNGHLVQAISTLFPFPEALEKLAFISDDAREPKGMSSIPTDILDAPKEYVF
Sbjct: 1   MSRAADSNSSNGHLVQAISTLFPFPEALEKLAFISDDAREPKGMSSIPTDILDAPKEYVF 60

Query: 61  YMDLPGLSKSDIQVTVEEENTLVIRSGGKRKREDGEEEGCKYLRLERKAPQKMVRKFRLP 120
           YMDLPGLSKSDIQVTVEEENTLVIRSGGKRKREDGEEEGCKYLRLERKAPQKMVRKFRLP
Sbjct: 61  YMDLPGLSKSDIQVTVEEENTLVIRSGGKRKREDGEEEGCKYLRLERKAPQKMVRKFRLP 120

Query: 121 ENADTYAVSAKCESGVLVVVVGKLPPPPKPKTVQVAVA 159
           ENADT AVSAKCESGVLVVVVGKLPPPPKPKTVQVAVA
Sbjct: 121 ENADTSAVSAKCESGVLVVVVGKLPPPPKPKTVQVAVA 158

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BLAST of Carg06384 vs. NCBI nr
Match: XP_023527030.1 (17.4 kDa class III heat shock protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 303.1 bits (775), Expect = 1.4e-78
Identity = 156/158 (98.73%), Postives = 157/158 (99.37%), Query Frame = 0


Query: 1   MSRAADSNSSNGHLVQAISTLFPFPEALEKLAFISDDAREPKGMSSIPTDILDAPKEYVF 60
           MSRAADSNSSNGHLVQAISTLFPFPEALEKLAFIS+DAREPKGMSSIPTDILDAPKEYVF
Sbjct: 1   MSRAADSNSSNGHLVQAISTLFPFPEALEKLAFISNDAREPKGMSSIPTDILDAPKEYVF 60

Query: 61  YMDLPGLSKSDIQVTVEEENTLVIRSGGKRKREDGEEEGCKYLRLERKAPQKMVRKFRLP 120
           YMDLPGLSKSDIQVTVEEENTLVIRSGGKRKREDGEEEGCKYLRLERKAPQKMVRKFRLP
Sbjct: 61  YMDLPGLSKSDIQVTVEEENTLVIRSGGKRKREDGEEEGCKYLRLERKAPQKMVRKFRLP 120

Query: 121 ENADTYAVSAKCESGVLVVVVGKLPPPPKPKTVQVAVA 159
           ENADT AVSAKCESGVLVVVVGKLPPPPKPKTVQVAVA
Sbjct: 121 ENADTSAVSAKCESGVLVVVVGKLPPPPKPKTVQVAVA 158

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BLAST of Carg06384 vs. NCBI nr
Match: XP_022980741.1 (17.4 kDa class III heat shock protein [Cucurbita maxima])

HSP 1 Score: 301.6 bits (771), Expect = 4.0e-78
Identity = 154/158 (97.47%), Postives = 156/158 (98.73%), Query Frame = 0


Query: 1   MSRAADSNSSNGHLVQAISTLFPFPEALEKLAFISDDAREPKGMSSIPTDILDAPKEYVF 60
           MSRAADSNSSNGHLVQAISTLFPFPEALEKLAF+SDDAREPKGM+ IPTDILDAPKEYVF
Sbjct: 1   MSRAADSNSSNGHLVQAISTLFPFPEALEKLAFVSDDAREPKGMACIPTDILDAPKEYVF 60

Query: 61  YMDLPGLSKSDIQVTVEEENTLVIRSGGKRKREDGEEEGCKYLRLERKAPQKMVRKFRLP 120
           YMDLPGLSKSDIQVTVEEENTLVIRSGGKRKREDGEEEGCKYLRLERKAPQKMVRKFRLP
Sbjct: 61  YMDLPGLSKSDIQVTVEEENTLVIRSGGKRKREDGEEEGCKYLRLERKAPQKMVRKFRLP 120

Query: 121 ENADTYAVSAKCESGVLVVVVGKLPPPPKPKTVQVAVA 159
           ENADT AVSAKCESGVLVVVVGKLPPPPKPKTVQVAVA
Sbjct: 121 ENADTSAVSAKCESGVLVVVVGKLPPPPKPKTVQVAVA 158

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BLAST of Carg06384 vs. NCBI nr
Match: XP_022955938.1 (17.4 kDa class III heat shock protein [Cucurbita moschata])

HSP 1 Score: 300.1 bits (767), Expect = 1.2e-77
Identity = 155/158 (98.10%), Postives = 156/158 (98.73%), Query Frame = 0


Query: 1   MSRAADSNSSNGHLVQAISTLFPFPEALEKLAFISDDAREPKGMSSIPTDILDAPKEYVF 60
           MSRAADSNSSNGHLVQAISTLFP PEALEKLAFIS+DAREPKGMSSIPTDILDAPKEYVF
Sbjct: 1   MSRAADSNSSNGHLVQAISTLFPSPEALEKLAFISNDAREPKGMSSIPTDILDAPKEYVF 60

Query: 61  YMDLPGLSKSDIQVTVEEENTLVIRSGGKRKREDGEEEGCKYLRLERKAPQKMVRKFRLP 120
           YMDLPGLSKSDIQVTVEEENTLVIRSGGKRKREDGEEEGCKYLRLERKAPQKMVRKFRLP
Sbjct: 61  YMDLPGLSKSDIQVTVEEENTLVIRSGGKRKREDGEEEGCKYLRLERKAPQKMVRKFRLP 120

Query: 121 ENADTYAVSAKCESGVLVVVVGKLPPPPKPKTVQVAVA 159
           ENADT AVSAKCESGVLVVVVGKLPPPPKPKTVQVAVA
Sbjct: 121 ENADTSAVSAKCESGVLVVVVGKLPPPPKPKTVQVAVA 158

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BLAST of Carg06384 vs. ExPASy Swiss-Prot
Match: Q9SYG1 (17.4 kDa class III heat shock protein OS=Arabidopsis thaliana OX=3702 GN=HSP17.4B PE=2 SV=1)

HSP 1 Score: 166.0 bits (419), Expect = 3.4e-40
Identity = 89/151 (58.94%), Postives = 116/151 (76.82%), Query Frame = 0


Query: 17  AISTLFPFPEALEKLAF-------ISDDAREPKGMSSIPTDILDAPKEYVFYMDLPGLSK 76
           AI+  F  PEA+EKL          ++++R     ++IP DIL++PKEY+FY+D+PG+SK
Sbjct: 5   AINHFFGLPEAIEKLILPISRSGESNNESRGRGSSNNIPIDILESPKEYIFYLDIPGISK 64

Query: 77  SDIQVTVEEENTLVIRSGGKRKREDGE-EEGCKYLRLERKAPQKMVRKFRLPENADTYAV 136
           SDIQVTVEEE TLVI+S GKRKR+D E EEG KY+RLER+  Q +V+KFRLPE+AD  +V
Sbjct: 65  SDIQVTVEEERTLVIKSNGKRKRDDDESEEGSKYIRLERRLAQNLVKKFRLPEDADMASV 124

Query: 137 SAKCESGVLVVVVGKLPP-PPKPKTVQVAVA 159
           +AK + GVL VV+ KLPP PPKPKTVQ+AV+
Sbjct: 125 TAKYQEGVLTVVIKKLPPQPPKPKTVQIAVS 155

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BLAST of Carg06384 vs. ExPASy Swiss-Prot
Match: Q6K7E9 (18.6 kDa class III heat shock protein OS=Oryza sativa subsp. japonica OX=39947 GN=HSP18.6 PE=2 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 3.6e-29
Identity = 80/166 (48.19%), Postives = 104/166 (62.65%), Query Frame = 0


Query: 17  AISTLFPFPEALEKLAFISDDAREP----------KGMSSI-------PTDILDAPKEYV 76
           A+++L   PE L++L   + D R             G   I       P DI++ P EY 
Sbjct: 8   AVTSLLHLPEVLDRLGAAAGDRRSAGDHAHHAAHGHGQHRISGIGGGAPVDIMETPGEYA 67

Query: 77  FYMDLPGLSKSDIQVTVEEENTLVIRS-----GGKRKREDGEEEGCKYLRLERKA-PQKM 136
           F +D+PGLSKSDIQVT+EE+  LV++S      GKRKRE+ EE  CKY+RLER+A P+  
Sbjct: 68  FVLDVPGLSKSDIQVTLEEDRVLVMKSSNGAGNGKRKREE-EEGECKYIRLERRASPRAF 127

Query: 137 VRKFRLPENADTYAVSAKCESGVLVVVVGKLPPP-PKPKTVQVAVA 159
            RKFRLPE+ADT  +SA+CE+GVL V V K PPP  K K+VQV +A
Sbjct: 128 ARKFRLPEDADTGGISARCENGVLTVTVKKRPPPEKKTKSVQVTIA 172

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BLAST of Carg06384 vs. ExPASy Swiss-Prot
Match: O82013 (17.3 kDa class II heat shock protein OS=Solanum peruvianum OX=4082 PE=2 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 3.7e-26
Identity = 65/122 (53.28%), Postives = 88/122 (72.13%), Query Frame = 0


Query: 39  REPKGMSSIPTDILDAPKEYVFYMDLPGLSKSDIQVTVEEENTLVIRSGGKRKREDGEEE 98
           R+ K M++ P D+ + P  YVF +D+PGL   DI+V VEE+N L+I   G+RKRE+ E+E
Sbjct: 38  RDAKAMAATPADVKEYPNSYVFVVDMPGLKSGDIKVQVEEDNVLLI--SGERKREE-EKE 97

Query: 99  GCKYLRLERKAPQKMVRKFRLPENADTYAVSAKCESGVLVVVVGKLPP--PPKPKTVQVA 158
           G K++R+ER+   K +RKF LPENA+T A+SA C+ GVL V V KLPP  P KPKT++V 
Sbjct: 98  GAKFIRMERRV-GKFMRKFSLPENANTDAISAVCQDGVLTVTVQKLPPPEPKKPKTIEVK 155

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BLAST of Carg06384 vs. ExPASy Swiss-Prot
Match: P05477 (17.9 kDa class II heat shock protein OS=Glycine max OX=3847 GN=HSP17.9-D PE=3 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 1.8e-25
Identity = 66/122 (54.10%), Postives = 88/122 (72.13%), Query Frame = 0


Query: 39  REPKGMSSIPTDILDAPKEYVFYMDLPGLSKSDIQVTVEEENTLVIRSGGKRKREDGEEE 98
           R+ K M++ P D+ + P  YVF +D+PGL   DI+V VE++N L+I   G+RKR D E+E
Sbjct: 42  RDAKAMAATPADVKEYPNSYVFEIDMPGLKSGDIKVQVEDDNLLLI--CGERKR-DEEKE 101

Query: 99  GCKYLRLERKAPQKMVRKFRLPENADTYAVSAKCESGVLVVVVGKLPP--PPKPKTVQVA 158
           G KYLR+ER+   K++RKF LPENA+T A+SA C+ GVL V V KLPP  P KP+T+QV 
Sbjct: 102 GAKYLRMERRV-GKLMRKFVLPENANTDAISAVCQDGVLSVTVQKLPPPEPKKPRTIQVK 159

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BLAST of Carg06384 vs. ExPASy Swiss-Prot
Match: O81822 (17.7 kDa class II heat shock protein OS=Arabidopsis thaliana OX=3702 GN=HSP17.7 PE=2 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 4.1e-25
Identity = 60/122 (49.18%), Postives = 85/122 (69.67%), Query Frame = 0


Query: 39  REPKGMSSIPTDILDAPKEYVFYMDLPGLSKSDIQVTVEEENTLVIRSGGKRKREDGEEE 98
           R+ K M++ P D+++ P  YVF +D+PG+   +IQV +E EN LV+   GKR+R++ E E
Sbjct: 38  RDAKAMAATPADVIEHPDAYVFAVDMPGIKGDEIQVQIENENVLVV--SGKRQRDNKENE 97

Query: 99  GCKYLRLERKAPQKMVRKFRLPENADTYAVSAKCESGVLVVVVGKLPP--PPKPKTVQVA 158
           G K++R+ER+   K +RKF+LP+NAD   +SA C  GVL V + KLPP  P KPKT+QV 
Sbjct: 98  GVKFVRMERRM-GKFMRKFQLPDNADLEKISAACNDGVLKVTIPKLPPPEPKKPKTIQVQ 156

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BLAST of Carg06384 vs. ExPASy TrEMBL
Match: A0A6J1IUF4 (17.4 kDa class III heat shock protein OS=Cucurbita maxima OX=3661 GN=LOC111480018 PE=3 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 1.9e-78
Identity = 154/158 (97.47%), Postives = 156/158 (98.73%), Query Frame = 0


Query: 1   MSRAADSNSSNGHLVQAISTLFPFPEALEKLAFISDDAREPKGMSSIPTDILDAPKEYVF 60
           MSRAADSNSSNGHLVQAISTLFPFPEALEKLAF+SDDAREPKGM+ IPTDILDAPKEYVF
Sbjct: 1   MSRAADSNSSNGHLVQAISTLFPFPEALEKLAFVSDDAREPKGMACIPTDILDAPKEYVF 60

Query: 61  YMDLPGLSKSDIQVTVEEENTLVIRSGGKRKREDGEEEGCKYLRLERKAPQKMVRKFRLP 120
           YMDLPGLSKSDIQVTVEEENTLVIRSGGKRKREDGEEEGCKYLRLERKAPQKMVRKFRLP
Sbjct: 61  YMDLPGLSKSDIQVTVEEENTLVIRSGGKRKREDGEEEGCKYLRLERKAPQKMVRKFRLP 120

Query: 121 ENADTYAVSAKCESGVLVVVVGKLPPPPKPKTVQVAVA 159
           ENADT AVSAKCESGVLVVVVGKLPPPPKPKTVQVAVA
Sbjct: 121 ENADTSAVSAKCESGVLVVVVGKLPPPPKPKTVQVAVA 158

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BLAST of Carg06384 vs. ExPASy TrEMBL
Match: A0A6J1GVE0 (17.4 kDa class III heat shock protein OS=Cucurbita moschata OX=3662 GN=LOC111457774 PE=3 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 5.6e-78
Identity = 155/158 (98.10%), Postives = 156/158 (98.73%), Query Frame = 0


Query: 1   MSRAADSNSSNGHLVQAISTLFPFPEALEKLAFISDDAREPKGMSSIPTDILDAPKEYVF 60
           MSRAADSNSSNGHLVQAISTLFP PEALEKLAFIS+DAREPKGMSSIPTDILDAPKEYVF
Sbjct: 1   MSRAADSNSSNGHLVQAISTLFPSPEALEKLAFISNDAREPKGMSSIPTDILDAPKEYVF 60

Query: 61  YMDLPGLSKSDIQVTVEEENTLVIRSGGKRKREDGEEEGCKYLRLERKAPQKMVRKFRLP 120
           YMDLPGLSKSDIQVTVEEENTLVIRSGGKRKREDGEEEGCKYLRLERKAPQKMVRKFRLP
Sbjct: 61  YMDLPGLSKSDIQVTVEEENTLVIRSGGKRKREDGEEEGCKYLRLERKAPQKMVRKFRLP 120

Query: 121 ENADTYAVSAKCESGVLVVVVGKLPPPPKPKTVQVAVA 159
           ENADT AVSAKCESGVLVVVVGKLPPPPKPKTVQVAVA
Sbjct: 121 ENADTSAVSAKCESGVLVVVVGKLPPPPKPKTVQVAVA 158

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BLAST of Carg06384 vs. ExPASy TrEMBL
Match: H6TB33 (HSP19 OS=Citrullus lanatus OX=3654 PE=2 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 1.3e-66
Identity = 132/153 (86.27%), Postives = 140/153 (91.50%), Query Frame = 0


Query: 1   MSRAADSNSSNGHLVQAISTLFPFPEALEKLAFIS--DDAREPKGMSSIPTDILDAPKEY 60
           MSRAADSNSSNGHLVQA+STLFPFPE LEK  F S   DAR+PK +S+IPTDILD P EY
Sbjct: 1   MSRAADSNSSNGHLVQAVSTLFPFPETLEKFVFNSGAHDARDPKAISTIPTDILDTPSEY 60

Query: 61  VFYMDLPGLSKSDIQVTVEEENTLVIRSGGKRKREDGEEEGCKYLRLERKAPQKMVRKFR 120
           +FYMDLPGLSKSDIQVTVEEENTLVIRSGGKRKREDGEEEGCKYLRLERKAPQK +RKFR
Sbjct: 61  IFYMDLPGLSKSDIQVTVEEENTLVIRSGGKRKREDGEEEGCKYLRLERKAPQKAMRKFR 120

Query: 121 LPENADTYAVSAKCESGVLVVVVGKLPPPPKPK 152
           LPENAD  A+SAKCESGVL+VVVGKLPPPPKPK
Sbjct: 121 LPENADVSAISAKCESGVLMVVVGKLPPPPKPK 153

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BLAST of Carg06384 vs. ExPASy TrEMBL
Match: A0A6P4AT58 (17.4 kDa class III heat shock protein OS=Ziziphus jujuba OX=326968 GN=LOC107435148 PE=3 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 1.5e-54
Identity = 111/160 (69.38%), Postives = 135/160 (84.38%), Query Frame = 0


Query: 1   MSRAADSNSSNGHLVQAISTLFPFPEALEKLAFIS--DDAREPKGMSSIPTDILDAPKEY 60
           MSRAADS++ NG L  A++  F FPE LEK  F S   +  + KG+SSIP DILDA KEY
Sbjct: 1   MSRAADSSAFNGDLASAVNNFFNFPETLEKFVFPSRAHEHNDNKGVSSIPVDILDAQKEY 60

Query: 61  VFYMDLPGLSKSDIQVTVEEENTLVIRSGGKRKREDGEEEGCKYLRLERKAPQKMVRKFR 120
           +F+MD+PGLSKSDIQVTVE+E+TLVIRSGGKRKRE+GEEEGCKYLRLER+ P+K++RKFR
Sbjct: 61  IFFMDVPGLSKSDIQVTVEDESTLVIRSGGKRKREEGEEEGCKYLRLERRPPKKLLRKFR 120

Query: 121 LPENADTYAVSAKCESGVLVVVVGKLPPPPKPKTVQVAVA 159
           LPENA+  A++AKCE+GVL VVV KLPPPPKPKTV+V ++
Sbjct: 121 LPENANVSAITAKCENGVLTVVVEKLPPPPKPKTVEVTIS 160

[Close]

BLAST of Carg06384 vs. ExPASy TrEMBL
Match: A0A6P5T8I9 (17.4 kDa class III heat shock protein OS=Prunus avium OX=42229 GN=LOC110764812 PE=3 SV=1)

HSP 1 Score: 220.7 bits (561), Expect = 4.3e-54
Identity = 111/161 (68.94%), Postives = 133/161 (82.61%), Query Frame = 0


Query: 1   MSRAADSNSSNGHLVQAISTLFPFPEALEKLAFIS---DDAREPKGMSSIPTDILDAPKE 60
           MSR ADS++ NG    A++ L  FPE ++K  F S   +   E KG +SIP DILD+PKE
Sbjct: 1   MSRVADSSAFNGDFATAVNQLLNFPETIDKFMFPSRAHETNNENKGAASIPVDILDSPKE 60

Query: 61  YVFYMDLPGLSKSDIQVTVEEENTLVIRSGGKRKREDGEEEGCKYLRLERKAPQKMVRKF 120
           Y+F++DLPGLSKSDIQVTVE+ENTLVIRS GKRKREDGEEEGCKYLRLER+APQK+ RKF
Sbjct: 61  YIFFLDLPGLSKSDIQVTVEDENTLVIRSNGKRKREDGEEEGCKYLRLERRAPQKLSRKF 120

Query: 121 RLPENADTYAVSAKCESGVLVVVVGKLPPPPKPKTVQVAVA 159
           RLPENA+  A+SAKCE+GVL VVV KLPPPPKPKTV+V+++
Sbjct: 121 RLPENANVGAISAKCENGVLTVVVEKLPPPPKPKTVEVSIS 161

[Close]

BLAST of Carg06384 vs. TAIR 10
Match: AT1G54050.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 166.0 bits (419), Expect = 2.4e-41
Identity = 89/151 (58.94%), Postives = 116/151 (76.82%), Query Frame = 0


Query: 17  AISTLFPFPEALEKLAF-------ISDDAREPKGMSSIPTDILDAPKEYVFYMDLPGLSK 76
           AI+  F  PEA+EKL          ++++R     ++IP DIL++PKEY+FY+D+PG+SK
Sbjct: 5   AINHFFGLPEAIEKLILPISRSGESNNESRGRGSSNNIPIDILESPKEYIFYLDIPGISK 64

Query: 77  SDIQVTVEEENTLVIRSGGKRKREDGE-EEGCKYLRLERKAPQKMVRKFRLPENADTYAV 136
           SDIQVTVEEE TLVI+S GKRKR+D E EEG KY+RLER+  Q +V+KFRLPE+AD  +V
Sbjct: 65  SDIQVTVEEERTLVIKSNGKRKRDDDESEEGSKYIRLERRLAQNLVKKFRLPEDADMASV 124

Query: 137 SAKCESGVLVVVVGKLPP-PPKPKTVQVAVA 159
           +AK + GVL VV+ KLPP PPKPKTVQ+AV+
Sbjct: 125 TAKYQEGVLTVVIKKLPPQPPKPKTVQIAVS 155

[Close]

BLAST of Carg06384 vs. TAIR 10
Match: AT5G12030.1 (heat shock protein 17.6A )

HSP 1 Score: 115.9 bits (289), Expect = 2.9e-26
Identity = 60/122 (49.18%), Postives = 85/122 (69.67%), Query Frame = 0


Query: 39  REPKGMSSIPTDILDAPKEYVFYMDLPGLSKSDIQVTVEEENTLVIRSGGKRKREDGEEE 98
           R+ K M++ P D+++ P  YVF +D+PG+   +IQV +E EN LV+   GKR+R++ E E
Sbjct: 38  RDAKAMAATPADVIEHPDAYVFAVDMPGIKGDEIQVQIENENVLVV--SGKRQRDNKENE 97

Query: 99  GCKYLRLERKAPQKMVRKFRLPENADTYAVSAKCESGVLVVVVGKLPP--PPKPKTVQVA 158
           G K++R+ER+   K +RKF+LP+NAD   +SA C  GVL V + KLPP  P KPKT+QV 
Sbjct: 98  GVKFVRMERRM-GKFMRKFQLPDNADLEKISAACNDGVLKVTIPKLPPPEPKKPKTIQVQ 156

[Close]

BLAST of Carg06384 vs. TAIR 10
Match: AT5G12020.1 (17.6 kDa class II heat shock protein )

HSP 1 Score: 114.8 bits (286), Expect = 6.4e-26
Identity = 61/122 (50.00%), Postives = 84/122 (68.85%), Query Frame = 0


Query: 39  REPKGMSSIPTDILDAPKEYVFYMDLPGLSKSDIQVTVEEENTLVIRSGGKRKREDGEEE 98
           R+ K M++ P D+++ P  Y F +D+PG+   +I+V VE +N LV+   G+R+RE+ E E
Sbjct: 37  RDAKAMAATPADVIEHPNAYAFVVDMPGIKGDEIKVQVENDNVLVV--SGERQRENKENE 96

Query: 99  GCKYLRLERKAPQKMVRKFRLPENADTYAVSAKCESGVLVVVVGKLPP--PPKPKTVQVA 158
           G KY+R+ER+   K +RKF+LPENAD   +SA C  GVL V V KLPP  P KPKT+QV 
Sbjct: 97  GVKYVRMERRM-GKFMRKFQLPENADLDKISAVCHDGVLKVTVQKLPPPEPKKPKTIQVQ 155

[Close]

BLAST of Carg06384 vs. TAIR 10
Match: AT5G59720.1 (heat shock protein 18.2 )

HSP 1 Score: 78.2 bits (191), Expect = 6.7e-15
Identity = 51/122 (41.80%), Postives = 76/122 (62.30%), Query Frame = 0


Query: 38  AREPKGMSSIPTDILDAPKEYVFYMDLPGLSKSDIQVTVEEENTLVIRSGGKRKREDGEE 97
           AR+    ++   D  + P+ +VF  DLPGL K +++V VE++N L I   G+R +E+ EE
Sbjct: 43  ARDVAAFTNARVDWKETPEAHVFKADLPGLKKEEVKVEVEDKNVLQI--SGERSKEN-EE 102

Query: 98  EGCKYLRLERKAPQKMVRKFRLPENADTYAVSAKCESGVLVVVVGKLP-PPPKPKTVQVA 157
           +  K+ R+ER A  K +R+FRLPENA    V A  E+GVL VVV K P   P+ K++ ++
Sbjct: 103 KNDKWHRVER-ASGKFMRRFRLPENAKMEEVKATMENGVLTVVVPKAPEKKPQVKSIDIS 160

Query: 158 VA 159
            A
Sbjct: 163 GA 160

[Close]

BLAST of Carg06384 vs. TAIR 10
Match: AT1G53540.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 72.4 bits (176), Expect = 3.7e-13
Identity = 49/135 (36.30%), Postives = 75/135 (55.56%), Query Frame = 0


Query: 23  PFPEALEKLAFISDDAREPKGMSSIPTDILDAPKEYVFYMDLPGLSKSDIQVTVEEENTL 82
           PF   L      +  A +    ++   D  + P+ +VF  DLPGL K +++V VE+ N L
Sbjct: 26  PFEGFLTPSGLANAPAMDVAAFTNAKVDWRETPEAHVFKADLPGLRKEEVKVEVEDGNIL 85

Query: 83  VIRSGGKRKREDGEEEGCKYLRLERKAPQKMVRKFRLPENADTYAVSAKCESGVLVVVVG 142
            I   G+R  E+ EE+  K+ R+ER +  K  R+FRLPENA    + A  E+GVL V V 
Sbjct: 86  QI--SGERSNEN-EEKNDKWHRVERSS-GKFTRRFRLPENAKMEEIKASMENGVLSVTVP 145

Query: 143 KLP-PPPKPKTVQVA 157
           K+P   P+ K++ ++
Sbjct: 146 KVPEKKPEVKSIDIS 156

The following BLAST results are available for this feature:

BLAST of Carg06384 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7018234.1	3.3e-80	100.00	17.4 kDa class III heat shock protein, partial [Cucurbita argyrosperma subsp. ar...	[more]
KAG6581781.1	3.6e-79	99.37	17.4 kDa class III heat shock protein, partial [Cucurbita argyrosperma subsp. so...	[more]
XP_023527030.1	1.4e-78	98.73	17.4 kDa class III heat shock protein [Cucurbita pepo subsp. pepo]	[more]
XP_022980741.1	4.0e-78	97.47	17.4 kDa class III heat shock protein [Cucurbita maxima]	[more]
XP_022955938.1	1.2e-77	98.10	17.4 kDa class III heat shock protein [Cucurbita moschata]	[more]

BLAST of Carg06384 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SYG1	3.4e-40	58.94	17.4 kDa class III heat shock protein OS=Arabidopsis thaliana OX=3702 GN=HSP17.4...	[more]
Q6K7E9	3.6e-29	48.19	18.6 kDa class III heat shock protein OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
O82013	3.7e-26	53.28	17.3 kDa class II heat shock protein OS=Solanum peruvianum OX=4082 PE=2 SV=1	[more]
P05477	1.8e-25	54.10	17.9 kDa class II heat shock protein OS=Glycine max OX=3847 GN=HSP17.9-D PE=3 SV...	[more]
O81822	4.1e-25	49.18	17.7 kDa class II heat shock protein OS=Arabidopsis thaliana OX=3702 GN=HSP17.7 ...	[more]

BLAST of Carg06384 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IUF4	1.9e-78	97.47	17.4 kDa class III heat shock protein OS=Cucurbita maxima OX=3661 GN=LOC11148001...	[more]
A0A6J1GVE0	5.6e-78	98.10	17.4 kDa class III heat shock protein OS=Cucurbita moschata OX=3662 GN=LOC111457...	[more]
H6TB33	1.3e-66	86.27	HSP19 OS=Citrullus lanatus OX=3654 PE=2 SV=1	[more]
A0A6P4AT58	1.5e-54	69.38	17.4 kDa class III heat shock protein OS=Ziziphus jujuba OX=326968 GN=LOC1074351...	[more]
A0A6P5T8I9	4.3e-54	68.94	17.4 kDa class III heat shock protein OS=Prunus avium OX=42229 GN=LOC110764812 P...	[more]

BLAST of Carg06384 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G54050.1	2.4e-41	58.94	HSP20-like chaperones superfamily protein	[more]
AT5G12030.1	2.9e-26	49.18	heat shock protein 17.6A	[more]
AT5G12020.1	6.4e-26	50.00	17.6 kDa class II heat shock protein	[more]
AT5G59720.1	6.7e-15	41.80	heat shock protein 18.2	[more]
AT1G53540.1	3.7e-13	36.30	HSP20-like chaperones superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002068	Alpha crystallin/Hsp20 domain	PFAM	PF00011	HSP20	coord: 50..155 e-value: 1.5E-19 score: 70.0
IPR002068	Alpha crystallin/Hsp20 domain	PROSITE	PS01031	SHSP	coord: 40..158 score: 20.995663
IPR008978	HSP20-like chaperone	GENE3D	2.60.40.790		coord: 13..157 e-value: 4.3E-29 score: 103.1
IPR008978	HSP20-like chaperone	SUPERFAMILY	49764	HSP20-like chaperones	coord: 34..152
IPR031107	Small heat shock protein HSP20	PANTHER	PTHR11527	HEAT-SHOCK PROTEIN 20 FAMILY MEMBER	coord: 18..158
None	No IPR available	PANTHER	PTHR11527:SF297	17.4 KDA CLASS III HEAT SHOCK PROTEIN	coord: 18..158
None	No IPR available	CDD	cd06464	ACD_sHsps-like	coord: 50..135 e-value: 8.43743E-21 score: 79.1352

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg06384-RA	Carg06384-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051259	protein complex oligomerization
biological_process	GO:0006457	protein folding
biological_process	GO:0009408	response to heat
biological_process	GO:0042542	response to hydrogen peroxide
biological_process	GO:0009651	response to salt stress
molecular_function	GO:0043621	protein self-association
molecular_function	GO:0051082	unfolded protein binding