Carg06369 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg06369
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Pectinesterase
Location	Carg_Chr06: 7563693 .. 7564640 (+)
RNA-Seq Expression	Carg06369
Synteny	Carg06369

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGCCATTAAAAGCGTTTCGGTTCTCGTTCTTGTTCTGGTTCTTGGGTCGGCCATTTTTGCTTACTCGGATGACGAAGTGAAAAATTGGTGCAGCCAAACGCCATATCCAGCTCCATGTGAACATTTTCTCACGGCAAAAAAAACGGCCACAGAAAAAAACCGGCCAATCGCTTCAAAATCCCACTTCCTTAAAGTTCTGGTCGAATCGGCCATCGAGCGAGCCGTTTTGGCCCAAAAAAACGCCGTCGCTCTCGGCCCAAAGTGCCAAAACGACCGAGAAAAATCTGCATGGACCGACTGTATCGAGCTCTACGACCAAATCATTCACCGGCTCAACCGGACCGCCGGTCGGTGCTCTCCGGTCGATGTTCAGACCTGGCTCAGTGCTGCTCTCACAGCTCTCGACACGTGTCGAGCTGGGTTTGAGGAGCTTGGGGTTTCGGCTTTCGGCTACTCTTTAACGGCTAATAATGTTTCCAAGCTGATTAGTGATGGGTTGTCGGTGAATAAGCCGGCTTCGGCTGAGGGGTTTGAGCCGCCGAGTATGAATGAAGGGTTTCCGACGTGGGTTAAGCCGGGAGAAAGGAAGCTGTTGCAGTCGAGCGCTCCGAAAGCTAACGTGGTGGTGGCGAAGGATGGGTCTGGGAGATTTAGGACGGTGAGTGAGGCGGTCGCCGCCGCGAAGGGCGGAGGAAGGTTTGTGATTTATGTGAAGGCCGGGGTTTATAGTGAGAATGTTGAGATTAAGGCTAAGAATGTTATGTTGATCGGCGATGGCATCGGAAAAACTATCATCACCGGAAGTAGGAGCGTCGGCGGCGGTTCCACCACTTTTAAATCCGCCACCGTTGGTAATCCCCCCTTTCTTTTTTATTTTTTTTATAAATAAAATCATAATTAAAATGGGTTTAATTGGGATTTTAGTGAGGTTTAAGAATTCTAA

mRNA sequence

ATGGCAGCCATTAAAAGCGTTTCGGTTCTCGTTCTTGTTCTGGTTCTTGGGTCGGCCATTTTTGCTTACTCGGATGACGAAGTGAAAAATTGGTGCAGCCAAACGCCATATCCAGCTCCATGTGAACATTTTCTCACGGCAAAAAAAACGGCCACAGAAAAAAACCGGCCAATCGCTTCAAAATCCCACTTCCTTAAAGTTCTGGTCGAATCGGCCATCGAGCGAGCCGTTTTGGCCCAAAAAAACGCCGTCGCTCTCGGCCCAAAGTGCCAAAACGACCGAGAAAAATCTGCATGGACCGACTGTATCGAGCTCTACGACCAAATCATTCACCGGCTCAACCGGACCGCCGGTCGGTGCTCTCCGGTCGATGTTCAGACCTGGCTCAGTGCTGCTCTCACAGCTCTCGACACGTGTCGAGCTGGGTTTGAGGAGCTTGGGGTTTCGGCTTTCGGCTACTCTTTAACGGCTAATAATGTTTCCAAGCTGATTAGTGATGGGTTGTCGGTGAATAAGCCGGCTTCGGCTGAGGGGTTTGAGCCGCCGAGTATGAATGAAGGGTTTCCGACGTGGGTTAAGCCGGGAGAAAGGAAGCTGTTGCAGTCGAGCGCTCCGAAAGCTAACGTGGTGGTGGCGAAGGATGGGTCTGGGAGATTTAGGACGGTGAGTGAGGCGGTCGCCGCCGCGAAGGGCGGAGGAAGGTTTGTGATTTATGTGAAGGCCGGGGTTTATAGTGAGAATGTTGAGATTAAGGCTAAGAATGTTATGTTGATCGGCGATGGCATCGGAAAAACTATCATCACCGGAAGTAGGAGCGTCGGCGGCGGTTCCACCACTTTTAAATCCGCCACCGTTGAATTCTAA

Coding sequence (CDS)

ATGGCAGCCATTAAAAGCGTTTCGGTTCTCGTTCTTGTTCTGGTTCTTGGGTCGGCCATTTTTGCTTACTCGGATGACGAAGTGAAAAATTGGTGCAGCCAAACGCCATATCCAGCTCCATGTGAACATTTTCTCACGGCAAAAAAAACGGCCACAGAAAAAAACCGGCCAATCGCTTCAAAATCCCACTTCCTTAAAGTTCTGGTCGAATCGGCCATCGAGCGAGCCGTTTTGGCCCAAAAAAACGCCGTCGCTCTCGGCCCAAAGTGCCAAAACGACCGAGAAAAATCTGCATGGACCGACTGTATCGAGCTCTACGACCAAATCATTCACCGGCTCAACCGGACCGCCGGTCGGTGCTCTCCGGTCGATGTTCAGACCTGGCTCAGTGCTGCTCTCACAGCTCTCGACACGTGTCGAGCTGGGTTTGAGGAGCTTGGGGTTTCGGCTTTCGGCTACTCTTTAACGGCTAATAATGTTTCCAAGCTGATTAGTGATGGGTTGTCGGTGAATAAGCCGGCTTCGGCTGAGGGGTTTGAGCCGCCGAGTATGAATGAAGGGTTTCCGACGTGGGTTAAGCCGGGAGAAAGGAAGCTGTTGCAGTCGAGCGCTCCGAAAGCTAACGTGGTGGTGGCGAAGGATGGGTCTGGGAGATTTAGGACGGTGAGTGAGGCGGTCGCCGCCGCGAAGGGCGGAGGAAGGTTTGTGATTTATGTGAAGGCCGGGGTTTATAGTGAGAATGTTGAGATTAAGGCTAAGAATGTTATGTTGATCGGCGATGGCATCGGAAAAACTATCATCACCGGAAGTAGGAGCGTCGGCGGCGGTTCCACCACTTTTAAATCCGCCACCGTTGAATTCTAA

Protein sequence

MAAIKSVSVLVLVLVLGSAIFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIASKSHFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGRCSPVDVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFEPPSMNEGFPTWVKPGERKLLQSSAPKANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVKAGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATVEF

Homology

[Close]

BLAST of Carg06369 vs. NCBI nr
Match: KAG7028681.1 (putative pectinesterase/pectinesterase inhibitor 17, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 558.5 bits (1438), Expect = 3.3e-155
Identity = 287/287 (100.00%), Postives = 287/287 (100.00%), Query Frame = 0


Query: 1   MAAIKSVSVLVLVLVLGSAIFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIAS 60
           MAAIKSVSVLVLVLVLGSAIFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIAS
Sbjct: 1   MAAIKSVSVLVLVLVLGSAIFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIAS 60

Query: 61  KSHFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGRC 120
           KSHFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGRC
Sbjct: 61  KSHFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGRC 120

Query: 121 SPVDVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFE 180
           SPVDVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFE
Sbjct: 121 SPVDVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFE 180

Query: 181 PPSMNEGFPTWVKPGERKLLQSSAPKANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVK 240
           PPSMNEGFPTWVKPGERKLLQSSAPKANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVK
Sbjct: 181 PPSMNEGFPTWVKPGERKLLQSSAPKANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVK 240

Query: 241 AGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATVEF 288
           AGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATVEF
Sbjct: 241 AGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATVEF 287

[Close]

BLAST of Carg06369 vs. NCBI nr
Match: XP_023539131.1 (pectinesterase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 547.4 bits (1409), Expect = 7.6e-152
Identity = 281/285 (98.60%), Postives = 283/285 (99.30%), Query Frame = 0


Query: 1   MAAIKSVSVLVLVLVLGSAIFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIAS 60
           MAAIKSVSVLVLVLVLGSAIFAYSD+EVKNWCSQTPYPAPCEHFLTAKKT TEKNRPIAS
Sbjct: 1   MAAIKSVSVLVLVLVLGSAIFAYSDNEVKNWCSQTPYPAPCEHFLTAKKTGTEKNRPIAS 60

Query: 61  KSHFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGRC 120
           KSHFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGRC
Sbjct: 61  KSHFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGRC 120

Query: 121 SPVDVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFE 180
           SPVDVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFE
Sbjct: 121 SPVDVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFE 180

Query: 181 PPSMNEGFPTWVKPGERKLLQSSAPKANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVK 240
           PPSMNEGFPTWVKPGERKLLQSSAP ANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVK
Sbjct: 181 PPSMNEGFPTWVKPGERKLLQSSAPNANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVK 240

Query: 241 AGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV 286
           AGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTF+SATV
Sbjct: 241 AGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFRSATV 285

[Close]

BLAST of Carg06369 vs. NCBI nr
Match: KAG6597210.1 (Pectinesterase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 544.3 bits (1401), Expect = 6.4e-151
Identity = 282/285 (98.95%), Postives = 282/285 (98.95%), Query Frame = 0


Query: 1   MAAIKSVSVLVLVLVLGSAIFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIAS 60
           MAAIK  SVLVLVLVLGSAIFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIAS
Sbjct: 1   MAAIK--SVLVLVLVLGSAIFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIAS 60

Query: 61  KSHFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGRC 120
           KSHFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGRC
Sbjct: 61  KSHFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGRC 120

Query: 121 SPVDVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFE 180
           SPVDVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFE
Sbjct: 121 SPVDVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFE 180

Query: 181 PPSMNEGFPTWVKPGERKLLQSSAPKANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVK 240
           PPSMNEGFPTWVKPGERKLLQSSAP ANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVK
Sbjct: 181 PPSMNEGFPTWVKPGERKLLQSSAPNANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVK 240

Query: 241 AGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV 286
           AGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV
Sbjct: 241 AGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV 283

[Close]

BLAST of Carg06369 vs. NCBI nr
Match: XP_022941906.1 (pectinesterase 2 [Cucurbita moschata])

HSP 1 Score: 541.2 bits (1393), Expect = 5.4e-150
Identity = 280/285 (98.25%), Postives = 281/285 (98.60%), Query Frame = 0


Query: 1   MAAIKSVSVLVLVLVLGSAIFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIAS 60
           MAAIK  SVLVLVLVLGS IFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIAS
Sbjct: 1   MAAIK--SVLVLVLVLGSVIFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIAS 60

Query: 61  KSHFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGRC 120
           KSHFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGRC
Sbjct: 61  KSHFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGRC 120

Query: 121 SPVDVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFE 180
           SPVDVQTWLSAALTALDTC+AGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFE
Sbjct: 121 SPVDVQTWLSAALTALDTCQAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFE 180

Query: 181 PPSMNEGFPTWVKPGERKLLQSSAPKANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVK 240
           PPSMNEGFPTWVKPGERKLLQSSAP ANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVK
Sbjct: 181 PPSMNEGFPTWVKPGERKLLQSSAPNANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVK 240

Query: 241 AGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV 286
           AGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV
Sbjct: 241 AGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV 283

[Close]

BLAST of Carg06369 vs. NCBI nr
Match: XP_022975621.1 (probable pectinesterase/pectinesterase inhibitor 17 [Cucurbita maxima])

HSP 1 Score: 531.2 bits (1367), Expect = 5.6e-147
Identity = 271/285 (95.09%), Postives = 277/285 (97.19%), Query Frame = 0


Query: 1   MAAIKSVSVLVLVLVLGSAIFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIAS 60
           M AIKSV VLVLVLVL SAIFAYSD+EVKNWCSQTPYPAPCEHFLTAKKT T+KNRPIAS
Sbjct: 1   MVAIKSVIVLVLVLVLWSAIFAYSDEEVKNWCSQTPYPAPCEHFLTAKKTTTKKNRPIAS 60

Query: 61  KSHFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGRC 120
           KSHFL+VLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDC+ELYDQIIHRLNRTA RC
Sbjct: 61  KSHFLRVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCVELYDQIIHRLNRTADRC 120

Query: 121 SPVDVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFE 180
           SP+DVQTWLSAALTALDTCR GFEELGVSAFGY LTANNVSKLISDGLSVNKPAS+EGFE
Sbjct: 121 SPIDVQTWLSAALTALDTCRTGFEELGVSAFGYPLTANNVSKLISDGLSVNKPASSEGFE 180

Query: 181 PPSMNEGFPTWVKPGERKLLQSSAPKANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVK 240
           PPSMNEGFPTWVKPGERKLLQSSAP ANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVK
Sbjct: 181 PPSMNEGFPTWVKPGERKLLQSSAPNANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVK 240

Query: 241 AGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV 286
           AGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV
Sbjct: 241 AGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV 285

[Close]

BLAST of Carg06369 vs. ExPASy Swiss-Prot
Match: O22149 (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3702 GN=PME17 PE=2 SV=2)

HSP 1 Score: 314.7 bits (805), Expect = 1.1e-84
Identity = 161/282 (57.09%), Postives = 207/282 (73.40%), Query Frame = 0


Query: 7   VSVLVLVLVLGSAIFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIASKSHFLK 66
           ++ ++L +++ S +  Y+  +VK WCSQTP P PCE+FL    T    N PI S+S FLK
Sbjct: 9   INFVILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFL----THNSNNEPIKSESEFLK 68

Query: 67  VLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAG---RCSPV 126
           + ++  ++RA+LA+ +A  LGPKC++ REK+AW DCI+LYD  + ++N T     +CS +
Sbjct: 69  ISMKLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKL 128

Query: 127 DVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFEPPS 186
           D QTWLS ALT LDTCRAGF ELGV+     L +NNVS L+ + L++NK      + PP 
Sbjct: 129 DAQTWLSTALTNLDTCRAGFLELGVTDIVLPLMSNNVSNLLCNTLAINKVPF--NYTPPE 188

Query: 187 MNEGFPTWVKPGERKLLQSSAPKANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVKAGV 246
             +GFP+WVKPG+RKLLQSS PK N VVAKDGSG F+T+ EA+ AA G GRFVIYVK GV
Sbjct: 189 -KDGFPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAASGSGRFVIYVKQGV 248

Query: 247 YSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV 286
           YSEN+EI+ KNVML GDGIGKTIITGS+SVGGG+TTF SATV
Sbjct: 249 YSENLEIRKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATV 283

[Close]

BLAST of Carg06369 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 4.7e-80
Identity = 155/284 (54.58%), Postives = 204/284 (71.83%), Query Frame = 0


Query: 7   VSVLVLVLVLGSAIFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIASKSHFLK 66
           ++V +++  L    F YS +EVK+WC +TP P PCE+FLT K   T     I   + F K
Sbjct: 7   ITVSLVLFSLSHTSFGYSPEEVKSWCGKTPNPQPCEYFLTQKTDVTS----IKQDTDFYK 66

Query: 67  VLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGR---CSPV 126
           + ++ A+ERA  AQ     LG KC+N+REK+AW DC ELY+  + +LN+T+     C+ V
Sbjct: 67  ISLQLALERATTAQSRTYTLGSKCRNEREKAAWEDCRELYELTVLKLNQTSNSSPGCTKV 126

Query: 127 DVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFEPPS 186
           D QTWLS ALT L+TCRA  E+LGV  +   L +NNV+KLIS+ LS+NK      +  PS
Sbjct: 127 DKQTWLSTALTNLETCRASLEDLGVPEYVLPLLSNNVTKLISNTLSLNKVP----YNEPS 186

Query: 187 MNEGFPTWVKPGERKLLQSSAPKANVVVAKDGSGRFRTVSEAVAAAK--GGGRFVIYVKA 246
             +GFPTWVKPG+RKLLQ++ P+AN+VVA+DGSG  +T+ EAVAAA   GG R+VIY+KA
Sbjct: 187 YKDGFPTWVKPGDRKLLQTT-PRANIVVAQDGSGNVKTIQEAVAAASRAGGSRYVIYIKA 246

Query: 247 GVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV 286
           G Y+EN+E+K KN+M +GDGIGKTIITGS+SVGGG+TTFKSATV
Sbjct: 247 GTYNENIEVKLKNIMFVGDGIGKTIITGSKSVGGGATTFKSATV 281

[Close]

BLAST of Carg06369 vs. ExPASy Swiss-Prot
Match: Q9FHN4 (Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3702 GN=PME60 PE=2 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 3.1e-47
Identity = 121/299 (40.47%), Postives = 168/299 (56.19%), Query Frame = 0


Query: 6   SVSVLVLVLVLGSAIFAYSDDEVKN---WCSQTPYPAPCEHFLTAKKTATEKNRPIASKS 65
           S+ +L+++L+    +   +D    N   WC +TPYP PC+ +           R     S
Sbjct: 13  SLHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYFIKHSGF----RLPTQIS 72

Query: 66  HFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNR------- 125
            F  +LVE+A++RAV A          C + ++++   DCI LY   + +LNR       
Sbjct: 73  EFRVLLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSS 132

Query: 126 -TAGRCSPVDVQTWLSAALTALDTCRAGFEELGVSAFGYSLTAN-NVSKLISDGLSVNKP 185
            T  RC+  D QTWLS ALT  +TCR G  +L VS F   + +N  +S LIS+ L+VN  
Sbjct: 133 KTGRRCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVNGA 192

Query: 186 ASAEGFEPPSM--NEGFPTWVKPGERKLLQSSAPKANVVVAKDGSGRFRTVSEAVAAA-- 245
               G    +   ++GFPTWV   ER+LLQ  + +AN+VVAKDGSG F+TV  A+  A  
Sbjct: 193 LLTAGKNDSTTGDSKGFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAAIDVAGR 252

Query: 246 --KGGGRFVIYVKAGVYSENVEIKAK--NVMLIGDGIGKTIITGSRSVGGGSTTFKSAT 285
                GRFVIYVK G+Y EN+ ++    N+ML+GDG+  TIITG RSV GG TT+ SAT
Sbjct: 253 RKVTSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTYSSAT 307

[Close]

BLAST of Carg06369 vs. ExPASy Swiss-Prot
Match: Q9FHN5 (Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3702 GN=PME59 PE=2 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 1.2e-46
Identity = 119/301 (39.53%), Postives = 173/301 (57.48%), Query Frame = 0


Query: 3   AIKSVSVLVLVLVLGSAIFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIASKS 62
           +I  + +++LVL+    +   +D    +WC +TPYP PC+ +    K      +P    S
Sbjct: 9   SILFLHLILLVLLCVHPLTTVADRNSTDWCDKTPYPDPCKCYF---KNHNGFQQP-TQLS 68

Query: 63  HFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRT------ 122
            F  +LVE+A++RA+ A+      G  C + ++++   DCI+LY   I +LNRT      
Sbjct: 69  EFRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGVSP 128

Query: 123 ----AGRCSPVDVQTWLSAALTALDTCRAGFEELGVSAFGYSLTAN-NVSKLISDGLSVN 182
               A  C+  D QTWLS ALT  +TCR G  +L V+ F   + +N  +S LIS+ L+VN
Sbjct: 129 KAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCLAVN 188

Query: 183 KPASAEGFE--PPSMNEGFPTWVKPGERKLLQSSAPKANVVVAKDGSGRFRTVSEAVAAA 242
                 G +    +  +GFPTW+   +++LL+  A +AN+VVAKDGSG F TV  A+  A
Sbjct: 189 GALLTAGNKGNTTANQKGFPTWLSRKDKRLLR--AVRANLVVAKDGSGHFNTVQAAIDVA 248

Query: 243 ----KGGGRFVIYVKAGVYSENVEIKAKN--VMLIGDGIGKTIITGSRSVGGGSTTFKSA 285
                  GRFVIYVK G+Y EN+ ++  N  +ML+GDG+  TIITG RSV GG TT+ SA
Sbjct: 249 GRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYNSA 303

[Close]

BLAST of Carg06369 vs. ExPASy Swiss-Prot
Match: Q9STY3 (Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3702 GN=PME33 PE=2 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 9.9e-46
Identity = 110/278 (39.57%), Postives = 161/278 (57.91%), Query Frame = 0


Query: 31  WCSQTPYPAPCEHFLTAKKTATEKNRPIASKSHFLKVLVESAIERAVLAQKNAVALGPKC 90
           WC++TP+   C ++           RP   +S FL++LV+ A+++AV+     V  GP C
Sbjct: 87  WCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTVKFGPSC 146

Query: 91  QNDREKSAWTDCIELYDQIIHRLNRTAG----------RCSPVDVQTWLSAALTALDTCR 150
            N++ K+AW+DC+ L+   + +LNRT            +C+  D QTWLS A T ++TCR
Sbjct: 147 TNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQTNIETCR 206

Query: 151 AGFEELGVSAFGYSLTAN-NVSKLISDGLSVNKPASAEGFEPPSMN--EGFPTWVKPGER 210
           +G E+L VS F   + +N N+S LI + L+VN     +     + N  E FP+WV   ER
Sbjct: 207 SGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEYFPSWVSRHER 266

Query: 211 KLLQSSA---PKANVVVAKDGSGRFRTVSEAV---AAAKGGGRFVIYVKAGVYSENVEI- 270
           +LL S++      ++VVA+D SG FR++  A+   A  +   RFVIYVK GVY EN+++ 
Sbjct: 267 RLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYRENIDVG 326

Query: 271 -KAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATVEF 288
               N+ML+GDG  KTIIT  RSV  G TT+ SAT  F
Sbjct: 327 NDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGF 364

[Close]

BLAST of Carg06369 vs. ExPASy TrEMBL
Match: A0A6J1FMD8 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111447099 PE=3 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 2.6e-150
Identity = 280/285 (98.25%), Postives = 281/285 (98.60%), Query Frame = 0


Query: 1   MAAIKSVSVLVLVLVLGSAIFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIAS 60
           MAAIK  SVLVLVLVLGS IFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIAS
Sbjct: 1   MAAIK--SVLVLVLVLGSVIFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIAS 60

Query: 61  KSHFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGRC 120
           KSHFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGRC
Sbjct: 61  KSHFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGRC 120

Query: 121 SPVDVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFE 180
           SPVDVQTWLSAALTALDTC+AGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFE
Sbjct: 121 SPVDVQTWLSAALTALDTCQAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFE 180

Query: 181 PPSMNEGFPTWVKPGERKLLQSSAPKANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVK 240
           PPSMNEGFPTWVKPGERKLLQSSAP ANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVK
Sbjct: 181 PPSMNEGFPTWVKPGERKLLQSSAPNANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVK 240

Query: 241 AGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV 286
           AGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV
Sbjct: 241 AGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV 283

[Close]

BLAST of Carg06369 vs. ExPASy TrEMBL
Match: A0A6J1IEQ5 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111475333 PE=3 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 2.7e-147
Identity = 271/285 (95.09%), Postives = 277/285 (97.19%), Query Frame = 0


Query: 1   MAAIKSVSVLVLVLVLGSAIFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIAS 60
           M AIKSV VLVLVLVL SAIFAYSD+EVKNWCSQTPYPAPCEHFLTAKKT T+KNRPIAS
Sbjct: 1   MVAIKSVIVLVLVLVLWSAIFAYSDEEVKNWCSQTPYPAPCEHFLTAKKTTTKKNRPIAS 60

Query: 61  KSHFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGRC 120
           KSHFL+VLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDC+ELYDQIIHRLNRTA RC
Sbjct: 61  KSHFLRVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCVELYDQIIHRLNRTADRC 120

Query: 121 SPVDVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFE 180
           SP+DVQTWLSAALTALDTCR GFEELGVSAFGY LTANNVSKLISDGLSVNKPAS+EGFE
Sbjct: 121 SPIDVQTWLSAALTALDTCRTGFEELGVSAFGYPLTANNVSKLISDGLSVNKPASSEGFE 180

Query: 181 PPSMNEGFPTWVKPGERKLLQSSAPKANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVK 240
           PPSMNEGFPTWVKPGERKLLQSSAP ANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVK
Sbjct: 181 PPSMNEGFPTWVKPGERKLLQSSAPNANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVK 240

Query: 241 AGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV 286
           AGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV
Sbjct: 241 AGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV 285

[Close]

BLAST of Carg06369 vs. ExPASy TrEMBL
Match: A0A6J1IGV3 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111472765 PE=3 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 2.7e-147
Identity = 271/285 (95.09%), Postives = 277/285 (97.19%), Query Frame = 0


Query: 1   MAAIKSVSVLVLVLVLGSAIFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIAS 60
           M AIKSV VLVLVLVL SAIFAYSD+EVKNWCSQTPYPAPCEHFLTAKKT T+KNRPIAS
Sbjct: 1   MVAIKSVIVLVLVLVLWSAIFAYSDEEVKNWCSQTPYPAPCEHFLTAKKTTTKKNRPIAS 60

Query: 61  KSHFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGRC 120
           KSHFL+VLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDC+ELYDQIIHRLNRTA RC
Sbjct: 61  KSHFLRVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCVELYDQIIHRLNRTADRC 120

Query: 121 SPVDVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFE 180
           SP+DVQTWLSAALTALDTCR GFEELGVSAFGY LTANNVSKLISDGLSVNKPAS+EGFE
Sbjct: 121 SPIDVQTWLSAALTALDTCRTGFEELGVSAFGYPLTANNVSKLISDGLSVNKPASSEGFE 180

Query: 181 PPSMNEGFPTWVKPGERKLLQSSAPKANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVK 240
           PPSMNEGFPTWVKPGERKLLQSSAP ANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVK
Sbjct: 181 PPSMNEGFPTWVKPGERKLLQSSAPNANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVK 240

Query: 241 AGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV 286
           AGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV
Sbjct: 241 AGVYSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV 285

[Close]

BLAST of Carg06369 vs. ExPASy TrEMBL
Match: A0A0A0L7N4 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G126810 PE=3 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 9.4e-116
Identity = 205/277 (74.01%), Postives = 244/277 (88.09%), Query Frame = 0


Query: 9   VLVLVLVLGSAIFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIASKSHFLKVL 68
           V+++VL+  S I  YS+ E+KNWCSQTPYPAPCE FL  K  AT K  PI +KSHF ++L
Sbjct: 10  VVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTK--ATTKKTPITTKSHFFEIL 69

Query: 69  VESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGRCSPVDVQTW 128
           VE+A+ERAV A KNA++LGPKC+N +EK+AWTDC++LYDQII RLNRT+ RCSP D QTW
Sbjct: 70  VETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTSARCSPADAQTW 129

Query: 129 LSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFEPPSMNEGF 188
           LSAALTAL+TCR GFEELG+SAFGY LTANNVSKLISDGLSVNKPAS EG+EP +M +GF
Sbjct: 130 LSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPTTMTDGF 189

Query: 189 PTWVKPGERKLLQSSAPKANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVKAGVYSENV 248
           PTWV PG RKLLQS +PKA+VVVA+DGSG F+TV +A++AAKGGGRFVIY+K+GVY+EN+
Sbjct: 190 PTWVSPGNRKLLQSESPKADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYIKSGVYNENL 249

Query: 249 EIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV 286
           +IKAKNVM++GDGIGKTIITGSRSVGGGSTTF+SATV
Sbjct: 250 DIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATV 284

[Close]

BLAST of Carg06369 vs. ExPASy TrEMBL
Match: A0A5A7U603 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G002830 PE=3 SV=1)

HSP 1 Score: 421.8 bits (1083), Expect = 2.3e-114
Identity = 204/278 (73.38%), Postives = 248/278 (89.21%), Query Frame = 0


Query: 9   VLVLVLVLGSAIFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIASKSHFLKVL 68
           V+++VL+    +  YS+ E+KNWCSQTPYPAPCE FL   K +T+K+ PI +KSHF ++L
Sbjct: 10  VVLVVLIFPWDVLCYSEKEIKNWCSQTPYPAPCEEFLKT-KASTKKSTPITTKSHFFEIL 69

Query: 69  VESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAGRCSPVDVQTW 128
           VE+A+ERAV A KNA++LGPKC+N +EK+AWTDC++LYD+II RLNRT+ RC+P D QTW
Sbjct: 70  VETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDEIITRLNRTSDRCTPADAQTW 129

Query: 129 LSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFEPPS-MNEG 188
           LSAALTAL+TCR GF+ELG+SAFGY LTANNVSKLISDGLSVNKPAS EG+EP + M++G
Sbjct: 130 LSAALTALETCRTGFDELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPTTIMDDG 189

Query: 189 FPTWVKPGERKLLQSSAPKANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVKAGVYSEN 248
           FPTWV+PG+RKLLQS +PKANVVVA+DGSG F+TV EAVAAAKGGGRFVIYVK+GVY+EN
Sbjct: 190 FPTWVRPGDRKLLQSGSPKANVVVAQDGSGNFKTVKEAVAAAKGGGRFVIYVKSGVYNEN 249

Query: 249 VEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV 286
           ++IKAKNVM++GDGIGKTIITGSRSVGGGSTTF+SATV
Sbjct: 250 LDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATV 286

[Close]

BLAST of Carg06369 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 314.7 bits (805), Expect = 7.7e-86
Identity = 161/282 (57.09%), Postives = 207/282 (73.40%), Query Frame = 0


Query: 7   VSVLVLVLVLGSAIFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIASKSHFLK 66
           ++ ++L +++ S +  Y+  +VK WCSQTP P PCE+FL    T    N PI S+S FLK
Sbjct: 9   INFVILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFL----THNSNNEPIKSESEFLK 68

Query: 67  VLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRTAG---RCSPV 126
           + ++  ++RA+LA+ +A  LGPKC++ REK+AW DCI+LYD  + ++N T     +CS +
Sbjct: 69  ISMKLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKL 128

Query: 127 DVQTWLSAALTALDTCRAGFEELGVSAFGYSLTANNVSKLISDGLSVNKPASAEGFEPPS 186
           D QTWLS ALT LDTCRAGF ELGV+     L +NNVS L+ + L++NK      + PP 
Sbjct: 129 DAQTWLSTALTNLDTCRAGFLELGVTDIVLPLMSNNVSNLLCNTLAINKVPF--NYTPPE 188

Query: 187 MNEGFPTWVKPGERKLLQSSAPKANVVVAKDGSGRFRTVSEAVAAAKGGGRFVIYVKAGV 246
             +GFP+WVKPG+RKLLQSS PK N VVAKDGSG F+T+ EA+ AA G GRFVIYVK GV
Sbjct: 189 -KDGFPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAASGSGRFVIYVKQGV 248

Query: 247 YSENVEIKAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATV 286
           YSEN+EI+ KNVML GDGIGKTIITGS+SVGGG+TTF SATV
Sbjct: 249 YSENLEIRKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATV 283

[Close]

BLAST of Carg06369 vs. TAIR 10
Match: AT5G51500.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 190.3 bits (482), Expect = 2.2e-48
Identity = 121/299 (40.47%), Postives = 168/299 (56.19%), Query Frame = 0


Query: 6   SVSVLVLVLVLGSAIFAYSDDEVKN---WCSQTPYPAPCEHFLTAKKTATEKNRPIASKS 65
           S+ +L+++L+    +   +D    N   WC +TPYP PC+ +           R     S
Sbjct: 13  SLHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYFIKHSGF----RLPTQIS 72

Query: 66  HFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNR------- 125
            F  +LVE+A++RAV A          C + ++++   DCI LY   + +LNR       
Sbjct: 73  EFRVLLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSS 132

Query: 126 -TAGRCSPVDVQTWLSAALTALDTCRAGFEELGVSAFGYSLTAN-NVSKLISDGLSVNKP 185
            T  RC+  D QTWLS ALT  +TCR G  +L VS F   + +N  +S LIS+ L+VN  
Sbjct: 133 KTGRRCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVNGA 192

Query: 186 ASAEGFEPPSM--NEGFPTWVKPGERKLLQSSAPKANVVVAKDGSGRFRTVSEAVAAA-- 245
               G    +   ++GFPTWV   ER+LLQ  + +AN+VVAKDGSG F+TV  A+  A  
Sbjct: 193 LLTAGKNDSTTGDSKGFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAAIDVAGR 252

Query: 246 --KGGGRFVIYVKAGVYSENVEIKAK--NVMLIGDGIGKTIITGSRSVGGGSTTFKSAT 285
                GRFVIYVK G+Y EN+ ++    N+ML+GDG+  TIITG RSV GG TT+ SAT
Sbjct: 253 RKVTSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTYSSAT 307

[Close]

BLAST of Carg06369 vs. TAIR 10
Match: AT5G51490.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 188.3 bits (477), Expect = 8.3e-48
Identity = 119/301 (39.53%), Postives = 173/301 (57.48%), Query Frame = 0


Query: 3   AIKSVSVLVLVLVLGSAIFAYSDDEVKNWCSQTPYPAPCEHFLTAKKTATEKNRPIASKS 62
           +I  + +++LVL+    +   +D    +WC +TPYP PC+ +    K      +P    S
Sbjct: 9   SILFLHLILLVLLCVHPLTTVADRNSTDWCDKTPYPDPCKCYF---KNHNGFQQP-TQLS 68

Query: 63  HFLKVLVESAIERAVLAQKNAVALGPKCQNDREKSAWTDCIELYDQIIHRLNRT------ 122
            F  +LVE+A++RA+ A+      G  C + ++++   DCI+LY   I +LNRT      
Sbjct: 69  EFRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGVSP 128

Query: 123 ----AGRCSPVDVQTWLSAALTALDTCRAGFEELGVSAFGYSLTAN-NVSKLISDGLSVN 182
               A  C+  D QTWLS ALT  +TCR G  +L V+ F   + +N  +S LIS+ L+VN
Sbjct: 129 KAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCLAVN 188

Query: 183 KPASAEGFE--PPSMNEGFPTWVKPGERKLLQSSAPKANVVVAKDGSGRFRTVSEAVAAA 242
                 G +    +  +GFPTW+   +++LL+  A +AN+VVAKDGSG F TV  A+  A
Sbjct: 189 GALLTAGNKGNTTANQKGFPTWLSRKDKRLLR--AVRANLVVAKDGSGHFNTVQAAIDVA 248

Query: 243 ----KGGGRFVIYVKAGVYSENVEIKAKN--VMLIGDGIGKTIITGSRSVGGGSTTFKSA 285
                  GRFVIYVK G+Y EN+ ++  N  +ML+GDG+  TIITG RSV GG TT+ SA
Sbjct: 249 GRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYNSA 303

[Close]

BLAST of Carg06369 vs. TAIR 10
Match: AT3G47400.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 185.3 bits (469), Expect = 7.0e-47
Identity = 110/278 (39.57%), Postives = 161/278 (57.91%), Query Frame = 0


Query: 31  WCSQTPYPAPCEHFLTAKKTATEKNRPIASKSHFLKVLVESAIERAVLAQKNAVALGPKC 90
           WC++TP+   C ++           RP   +S FL++LV+ A+++AV+     V  GP C
Sbjct: 87  WCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTVKFGPSC 146

Query: 91  QNDREKSAWTDCIELYDQIIHRLNRTAG----------RCSPVDVQTWLSAALTALDTCR 150
            N++ K+AW+DC+ L+   + +LNRT            +C+  D QTWLS A T ++TCR
Sbjct: 147 TNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQTNIETCR 206

Query: 151 AGFEELGVSAFGYSLTAN-NVSKLISDGLSVNKPASAEGFEPPSMN--EGFPTWVKPGER 210
           +G E+L VS F   + +N N+S LI + L+VN     +     + N  E FP+WV   ER
Sbjct: 207 SGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEYFPSWVSRHER 266

Query: 211 KLLQSSA---PKANVVVAKDGSGRFRTVSEAV---AAAKGGGRFVIYVKAGVYSENVEI- 270
           +LL S++      ++VVA+D SG FR++  A+   A  +   RFVIYVK GVY EN+++ 
Sbjct: 267 RLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYRENIDVG 326

Query: 271 -KAKNVMLIGDGIGKTIITGSRSVGGGSTTFKSATVEF 288
               N+ML+GDG  KTIIT  RSV  G TT+ SAT  F
Sbjct: 327 NDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGF 364

[Close]

BLAST of Carg06369 vs. TAIR 10
Match: AT4G00190.1 (pectin methylesterase 38 )

HSP 1 Score: 167.5 bits (423), Expect = 1.5e-41
Identity = 113/263 (42.97%), Postives = 153/263 (58.17%), Query Frame = 0


Query: 32  CSQTPYPAPCEHFLTAKKTATEKNRPIASKSHFLKVLVESAIERAVLAQKNAVALGPKCQ 91
           C +TP+P  C       KT   K++PI S + FL+V VE  ++ AV A+ +   L P+  
Sbjct: 8   CDETPHPGEC-------KTLLIKHKPIRSTTQFLQVSVERTLDGAVKAKSDTYFLEPQFG 67

Query: 92  NDREKSAWTDCIELYDQIIHRLNRTA----GRCSPVDVQTWLSAALTALDTCRAGFEELG 151
           +   K AW +C++LY+Q IHRLN +       CS  DVQ WLS ALT LDTC+    ELG
Sbjct: 68  S---KQAWEECMDLYEQTIHRLNESVLCPKNVCSRSDVQAWLSTALTNLDTCQEEMSELG 127

Query: 152 VSAFGYSLTANNVSKLISDGLSVNKPASAEGFEPPSMNEGFPTWVKPGERKLLQSSAPKA 211
           VS+  +SL +  +   + + L++NK     G E           +     K L S   K 
Sbjct: 128 VSS--HSLESITID--VINTLAINKRMEQNGKE---------FGISKITMKTL-SIGEKV 187

Query: 212 NVVVAKDGSGRFRTVSEAVAAA----KGGGRFVIYVKAGVYSE--NVEIKAKNVMLIGDG 271
           +VVVA+DGSG ++T+ EAV  A    KG  R+VI+VK GVY E  NV IK+ N+M+ GDG
Sbjct: 188 DVVVAQDGSGDYKTIQEAVNGAGERLKGSPRYVIHVKQGVYEEYVNVGIKSNNIMITGDG 246

Query: 272 IGKTIITGSRSVGGGSTTFKSAT 285
           IGKTIITG +S G G +T+KSAT
Sbjct: 248 IGKTIITGDKSKGRGFSTYKSAT 246

The following BLAST results are available for this feature:

BLAST of Carg06369 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7028681.1	3.3e-155	100.00	putative pectinesterase/pectinesterase inhibitor 17, partial [Cucurbita argyrosp...	[more]
XP_023539131.1	7.6e-152	98.60	pectinesterase 2 [Cucurbita pepo subsp. pepo]	[more]
KAG6597210.1	6.4e-151	98.95	Pectinesterase 2, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022941906.1	5.4e-150	98.25	pectinesterase 2 [Cucurbita moschata]	[more]
XP_022975621.1	5.6e-147	95.09	probable pectinesterase/pectinesterase inhibitor 17 [Cucurbita maxima]	[more]

BLAST of Carg06369 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22149	1.1e-84	57.09	Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3...	[more]
O04887	4.7e-80	54.58	Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1	[more]
Q9FHN4	3.1e-47	40.47	Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3...	[more]
Q9FHN5	1.2e-46	39.53	Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3...	[more]
Q9STY3	9.9e-46	39.57	Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of Carg06369 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FMD8	2.6e-150	98.25	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111447099 PE=3 SV=1	[more]
A0A6J1IEQ5	2.7e-147	95.09	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111475333 PE=3 SV=1	[more]
A0A6J1IGV3	2.7e-147	95.09	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111472765 PE=3 SV=1	[more]
A0A0A0L7N4	9.4e-116	74.01	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G126810 PE=3 SV=1	[more]
A0A5A7U603	2.3e-114	73.38	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G00283...	[more]

BLAST of Carg06369 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G45220.1	7.7e-86	57.09	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G51500.1	2.2e-48	40.47	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G51490.1	8.3e-48	39.53	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G47400.1	7.0e-47	39.57	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G00190.1	1.5e-41	42.97	pectin methylesterase 38	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 23..170 e-value: 3.8E-31 score: 119.5
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 7..162 e-value: 3.0E-24 score: 83.3
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 27..167 e-value: 5.0E-24 score: 85.4
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 202..287 e-value: 1.9E-24 score: 88.6
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 22..172 e-value: 3.1E-24 score: 87.7
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 26..171
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 208..285 e-value: 2.6E-24 score: 85.5
None	No IPR available	PANTHER	PTHR31707:SF300	PECTINESTERASE	coord: 8..285
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 8..285
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 31..176 e-value: 4.45295E-29 score: 106.368
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 202..285

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg06369-RA	Carg06369-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity