Carg06361 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg06361
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Glutathione S-transferase
Location	Carg_Chr06: 7594534 .. 7596860 (-)
RNA-Seq Expression	Carg06361
Synteny	Carg06361

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGAAAGTGTTTATGAACGAAGGGAAGTTGAAAGAGGAAGGGATAGAAGAAGCGAAGGAGAATTTGAAGCATTTGGAAGAAGAGTTAAAGGCGGGGGGGAAGAGGTTCTTTGGCGGGGAAGTGATAGGGTTCGTGGATATTGCGCTTGGGTGGCTCGCAAACTTGCCAAGTGTGTTGGAGGAAGTGATTGGACTTAGCCTAATAACTCAACAAACCTTCCCCTTCTTGTCTAAATGGACTCACGAATTTGCTAATGCCCCAATCATCAATCAACATTGGCCTCCTAAACAACAACTTGTCACCAAGTTCAAAGCTATGCATCAAATTTTCATGAATAAACAAGCATGAGCGTAATTGATCTCCGATAAACCTCACGGGATAGGAGAAGCAGTGATTGGAGATTCCCTTGAATGGAGTCGAATGAGGGTAGATCTTAACGTTTGGTTTAAAGGGTAGTGTTATCCCGACCCCTACTGTCATCATGTCTTTACAGTAACGTTTATGATATTTTCATTTCGTCGTTGAAGAAGCTATTTCCTGCCTTGTTTAAACTAGGAGTGTTCAGTGGTTTAGGGTTTAGGTTCAGATATTTTTATACCGAACTGAATTGTTCAAGGAGTAAATTTTTCAACTTAAGTAACACTTATTGACTGATCAACATAGCCCTATCCTATCATAAAATATTGGGTTGGGTTGGGTTGGTCCAGGTTGTTCGGGTCGTTGATTCAAATTTGTAAGACCCAAAGAGATATCAGAAGGAAGGAAAGAAAGACCAACCTTTAGAATTTGATTCATAGGCTAGCATGAGAAGGCATAAGCAGTCGGAGAAATTTGCACGAATGCACACCATTGCCCGTAGATATTGCCTGCTTTAGTCCGTTACGCATTGTCGTCAGCCTCACGATTTTAAAACGCATCTGTTAAGAAGAGGTTTCCACAAACTTATAAGAAATGATTCGTTACTCTCTCCAACCAATATGAGATTTCTCAGTAGTAGTGCATGATAAATCATTCGAAAAATGATACACATTATATGTGCATTCTCGTAATCCTACCTACTTTGGGCAGCTCAATCACTTACTTTAAAACACTTCAACCGAGCTGAATCTGCTCGCTTTTAGTTATCTTCGACGAAAATAATAAGGAAAAGATAGCACGGACTTACAATGTTTACGTACATGTCAATGATCGATGAGCATAACTCGGCAAAAGTCGACCTTCTTGGATTTGCACCGAACTTGAACAAAGATCAAACAATTCATTAGGGTATTCACCAGTGATCTCCACAACTACAGGTTCCATCCTTGAAAAGATGAAATCGATTGGCATCTCTCACAAGTATTTCTCTTTGCATTATCTTAGTTATGTACTTGGTTACAGTATGACAATCTCCACATACTCTAAGGTTTTTCATTACCAGCAAAGGGCTCCCTGGTTTTGTATTCAACAACCCGAACGCTATAGCGAGTCTTTCGCTGTGTCCACATAGCATGTCTCTCTTATCGTCTTCTTCGACGTCGTGCAGAGCAAATTGCAGGTCTGCAACGTAACCTTCCTTGGCCATTAACCCCGTGAGATACTCGAGAAAATCTCTAACCTTATCGTATTGTTCAAACGACTTATCACTAGTTCGAAAAACATAAACTCTTTTCTGAATCTCAATCCAGCTACTTCCAGGCTCTTTCTTCAGCCCTTTATTTTTCATGGTATTTCTTACCAATCTCACCTGATCCCACTTCCCTAACGTAGCATAAACATTTGAAACAAGCACATAATACCCAGTATTGTCTGAGTTCAATTGAAGAATTTGGTTCGATACTCTTTGAGCGATATCCGTGTGCCCACTCGCTCGACAGGCGCTAAGTAGAGCTCCCCACAAACTTGCATCTGGTTTCATTGGCATTGACAGGATAAACTCCTCTGCTCGAGCTAATAAACCAGATCGAGCCAGAAGATCGACGACACAAGCATAATGTTCCATCCGAGGCTCAATATTGTAGTCGGTTTTCATTCGATCGAAGAATGCGAGCCCGTCTTCGACCATTCCAGAATGACTAAAAGCAAAAATGAGAGCAATGAAGGCAACTGAATCTGGAATAACACCACTTGACTCCATATCCTGAAAAGCCTTCAATGCTTTCTTGCCCTCGCCATACATTCCAAATGCAGAGATCAATGCAGTCCATGTCACTACATCTTTTTCTTTCATATAGTTGAACACTTTGGTACAGTTCTCTAAACTACCACATTTGGAGTACATTTCAATCAGGGCATTCCCAATTGGGACATCTAATTCCAATCCCAACTTGAAAATACAGCAATGA

mRNA sequence

ATGAAGAAAGTGTTTATGAACGAAGGGAAGTTGAAAGAGGAAGGGATAGAAGAAGCGAAGGAGAATTTGAAGCATTTGGAAGAAGAGTTAAAGGCGGGGGGGAAGAGGTTCTTTGGCGGGGAAGTGATAGGGTTCGTGGATATTGCGCTTGGGTGGCTCGCAAACTTGCCAAGTGTGTTGGAGGAAGTGATTGGACTTAGCCTAATAACTCAACAAACCTTCCCCTTCTTGTCTAAATGGACTCACGAATTTGCTAATGCCCCAATCATCAATCAACATTGGCCTCCTAAACAACAACTTGTCACCAATATGACAATCTCCACATACTCTAAGTTGAACACTTTGGTACAGTTCTCTAAACTACCACATTTGGAGTACATTTCAATCAGGGCATTCCCAATTGGGACATCTAATTCCAATCCCAACTTGAAAATACAGCAATGA

Coding sequence (CDS)

ATGAAGAAAGTGTTTATGAACGAAGGGAAGTTGAAAGAGGAAGGGATAGAAGAAGCGAAGGAGAATTTGAAGCATTTGGAAGAAGAGTTAAAGGCGGGGGGGAAGAGGTTCTTTGGCGGGGAAGTGATAGGGTTCGTGGATATTGCGCTTGGGTGGCTCGCAAACTTGCCAAGTGTGTTGGAGGAAGTGATTGGACTTAGCCTAATAACTCAACAAACCTTCCCCTTCTTGTCTAAATGGACTCACGAATTTGCTAATGCCCCAATCATCAATCAACATTGGCCTCCTAAACAACAACTTGTCACCAATATGACAATCTCCACATACTCTAAGTTGAACACTTTGGTACAGTTCTCTAAACTACCACATTTGGAGTACATTTCAATCAGGGCATTCCCAATTGGGACATCTAATTCCAATCCCAACTTGAAAATACAGCAATGA

Protein sequence

MKKVFMNEGKLKEEGIEEAKENLKHLEEELKAGGKRFFGGEVIGFVDIALGWLANLPSVLEEVIGLSLITQQTFPFLSKWTHEFANAPIINQHWPPKQQLVTNMTISTYSKLNTLVQFSKLPHLEYISIRAFPIGTSNSNPNLKIQQ

Homology

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BLAST of Carg06361 vs. NCBI nr
Match: KAG7028689.1 (putative glutathione S-transferase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 295.8 bits (756), Expect = 2.0e-76
Identity = 147/147 (100.00%), Postives = 147/147 (100.00%), Query Frame = 0


Query: 1   MKKVFMNEGKLKEEGIEEAKENLKHLEEELKAGGKRFFGGEVIGFVDIALGWLANLPSVL 60
           MKKVFMNEGKLKEEGIEEAKENLKHLEEELKAGGKRFFGGEVIGFVDIALGWLANLPSVL
Sbjct: 1   MKKVFMNEGKLKEEGIEEAKENLKHLEEELKAGGKRFFGGEVIGFVDIALGWLANLPSVL 60

Query: 61  EEVIGLSLITQQTFPFLSKWTHEFANAPIINQHWPPKQQLVTNMTISTYSKLNTLVQFSK 120
           EEVIGLSLITQQTFPFLSKWTHEFANAPIINQHWPPKQQLVTNMTISTYSKLNTLVQFSK
Sbjct: 61  EEVIGLSLITQQTFPFLSKWTHEFANAPIINQHWPPKQQLVTNMTISTYSKLNTLVQFSK 120

Query: 121 LPHLEYISIRAFPIGTSNSNPNLKIQQ 148
           LPHLEYISIRAFPIGTSNSNPNLKIQQ
Sbjct: 121 LPHLEYISIRAFPIGTSNSNPNLKIQQ 147

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BLAST of Carg06361 vs. NCBI nr
Match: KAG6597218.1 (hypothetical protein SDJN03_10398, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 207.6 bits (527), Expect = 7.3e-50
Identity = 102/102 (100.00%), Postives = 102/102 (100.00%), Query Frame = 0


Query: 1   MKKVFMNEGKLKEEGIEEAKENLKHLEEELKAGGKRFFGGEVIGFVDIALGWLANLPSVL 60
           MKKVFMNEGKLKEEGIEEAKENLKHLEEELKAGGKRFFGGEVIGFVDIALGWLANLPSVL
Sbjct: 77  MKKVFMNEGKLKEEGIEEAKENLKHLEEELKAGGKRFFGGEVIGFVDIALGWLANLPSVL 136

Query: 61  EEVIGLSLITQQTFPFLSKWTHEFANAPIINQHWPPKQQLVT 103
           EEVIGLSLITQQTFPFLSKWTHEFANAPIINQHWPPKQQLVT
Sbjct: 137 EEVIGLSLITQQTFPFLSKWTHEFANAPIINQHWPPKQQLVT 178

[Close]

BLAST of Carg06361 vs. NCBI nr
Match: XP_022974174.1 (probable glutathione S-transferase [Cucurbita maxima] >XP_022975554.1 probable glutathione S-transferase [Cucurbita maxima])

HSP 1 Score: 202.6 bits (514), Expect = 2.3e-48
Identity = 99/102 (97.06%), Postives = 100/102 (98.04%), Query Frame = 0


Query: 1   MKKVFMNEGKLKEEGIEEAKENLKHLEEELKAGGKRFFGGEVIGFVDIALGWLANLPSVL 60
           MKKVFM EGKLKEEGIEEAKENLKHLEEELKAGGKRFFGGEVIGFVD+ALGWLANLPSVL
Sbjct: 112 MKKVFMKEGKLKEEGIEEAKENLKHLEEELKAGGKRFFGGEVIGFVDVALGWLANLPSVL 171

Query: 61  EEVIGLSLITQQTFPFLSKWTHEFANAPIINQHWPPKQQLVT 103
           EEVIGLSLITQQTFP LSKWTHEFANAPIINQHWPPKQQLVT
Sbjct: 172 EEVIGLSLITQQTFPLLSKWTHEFANAPIINQHWPPKQQLVT 213

[Close]

BLAST of Carg06361 vs. NCBI nr
Match: XP_023540933.1 (probable glutathione S-transferase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 202.2 bits (513), Expect = 3.1e-48
Identity = 99/102 (97.06%), Postives = 101/102 (99.02%), Query Frame = 0


Query: 1   MKKVFMNEGKLKEEGIEEAKENLKHLEEELKAGGKRFFGGEVIGFVDIALGWLANLPSVL 60
           MKKVFM+EGKLKEEGIEEAKENLKHLEEELKAGGKRFFGGEVIGFVDIALGWLANLPSVL
Sbjct: 129 MKKVFMSEGKLKEEGIEEAKENLKHLEEELKAGGKRFFGGEVIGFVDIALGWLANLPSVL 188

Query: 61  EEVIGLSLITQQTFPFLSKWTHEFANAPIINQHWPPKQQLVT 103
           EEVIGL+LITQQTFP LSKWTHEFANAPIINQHWPPKQQLVT
Sbjct: 189 EEVIGLTLITQQTFPLLSKWTHEFANAPIINQHWPPKQQLVT 230

[Close]

BLAST of Carg06361 vs. NCBI nr
Match: XP_022950014.1 (probable glutathione S-transferase [Cucurbita moschata])

HSP 1 Score: 199.1 bits (505), Expect = 2.6e-47
Identity = 98/102 (96.08%), Postives = 100/102 (98.04%), Query Frame = 0


Query: 1   MKKVFMNEGKLKEEGIEEAKENLKHLEEELKAGGKRFFGGEVIGFVDIALGWLANLPSVL 60
           MK+VFMNEGKLKEEGIEEAKE+LKHLEEELKA GKRFFGGEVIGFVDIALGWLANLPSVL
Sbjct: 113 MKRVFMNEGKLKEEGIEEAKESLKHLEEELKARGKRFFGGEVIGFVDIALGWLANLPSVL 172

Query: 61  EEVIGLSLITQQTFPFLSKWTHEFANAPIINQHWPPKQQLVT 103
           EEVIGLSLITQQTFP LSKWTHEFANAPIINQHWPPKQQLVT
Sbjct: 173 EEVIGLSLITQQTFPLLSKWTHEFANAPIINQHWPPKQQLVT 214

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BLAST of Carg06361 vs. ExPASy Swiss-Prot
Match: P32110 (Probable glutathione S-transferase OS=Glycine max OX=3847 GN=HSP26-A PE=2 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 1.8e-14
Identity = 43/99 (43.43%), Postives = 60/99 (60.61%), Query Frame = 0


Query: 2   KKVFMNEGKLKEEGIEEAKENLKHLEEELKAGGKRFFGGEVIGFVDIALGWLANLPSVLE 61
           K VF  + K +E+ +EE  E L+ LE ELK   K+FFGGE  G VDIA  ++A    + +
Sbjct: 114 KSVFTVDEKEREKNVEETYEALQFLENELK--DKKFFGGEEFGLVDIAAVFIAFWIPIFQ 173

Query: 62  EVIGLSLITQQTFPFLSKWTHEFANAPIINQHWPPKQQL 101
           E+ GL L T + FP L KW+ EF N P +++  PP+  L
Sbjct: 174 EIAGLQLFTSEKFPILYKWSQEFLNHPFVHEVLPPRDPL 210

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BLAST of Carg06361 vs. ExPASy Swiss-Prot
Match: Q9SR36 (Glutathione S-transferase U8 OS=Arabidopsis thaliana OX=3702 GN=GSTU8 PE=2 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 1.6e-12
Identity = 40/101 (39.60%), Postives = 61/101 (60.40%), Query Frame = 0


Query: 1   MKKVFMNEGKLKEEGIEEAKENLKHLEEELKAGGKRFFGGEVIGFVDIALGWLANLPSVL 60
           +KK        +E+ ++EA E LK LE+EL  G K FFGGE IGFVDIA  ++     + 
Sbjct: 113 VKKACWGPESEREKEVKEAYEGLKCLEKEL--GDKLFFGGETIGFVDIAADFIGYWLGIF 172

Query: 61  EEVIGLSLITQQTFPFLSKWTHEFANAPIINQHWPPKQQLV 102
           +E  G++++T + FP L +W+ +F     I +  PPK++LV
Sbjct: 173 QEASGVTIMTAEEFPKLQRWSEDFVGNNFIKEVLPPKEKLV 211

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BLAST of Carg06361 vs. ExPASy Swiss-Prot
Match: Q9FQA3 (Glutathione transferase GST 23 OS=Zea mays OX=4577 PE=2 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 1.1e-11
Identity = 33/98 (33.67%), Postives = 58/98 (59.18%), Query Frame = 0


Query: 4   VFMNEGKLKEEGIEEAKENLKHLEEELKAGGKRFFGGEVIGFVDIALGWLANLPSVLEEV 63
           +F   G+ + + + EA++ LK LE  L+  GK+FFGG+ +G++DI +GW A+   V+EEV
Sbjct: 114 IFTATGEAQRKAVHEAQQCLKTLETALE--GKKFFGGDAVGYLDIVVGWFAHWLPVIEEV 173

Query: 64  IGLSLITQQTFPFLSKWTHEFANAPIINQHWPPKQQLV 102
            G S++T +  P +  W   F    ++    P + +L+
Sbjct: 174 TGASVVTHEELPLMKAWFGRFLALDVVKAALPDRDRLL 209

[Close]

BLAST of Carg06361 vs. ExPASy Swiss-Prot
Match: Q03662 (Probable glutathione S-transferase OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 9.0e-11
Identity = 39/99 (39.39%), Postives = 56/99 (56.57%), Query Frame = 0


Query: 3   KVFMNEGKLKEEGIEEAKENLKHLEEELKAGGKRFFGGEVIGFVDIALGWLANLPSVLEE 62
           K F+ +G+ +E+  EE  E LK L+ ELK   K+FF G+  GF DIA   +A    V EE
Sbjct: 109 KTFLRKGEEQEKDKEEVCEMLKVLDNELK--DKKFFVGDKFGFADIAANLVAFWLGVFEE 168

Query: 63  VIGLSLITQQTFPFLSKWTHEFANAPIINQHWPPKQQLV 102
             G+ L+T + FP   KW  E+ N   I +  PP+ +L+
Sbjct: 169 ASGVVLVTSEKFPNFCKWRGEYINCSQIKESLPPRDELL 205

[Close]

BLAST of Carg06361 vs. ExPASy Swiss-Prot
Match: Q03663 (Probable glutathione S-transferase OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.2e-10
Identity = 38/97 (39.18%), Postives = 54/97 (55.67%), Query Frame = 0


Query: 5   FMNEGKLKEEGIEEAKENLKHLEEELKAGGKRFFGGEVIGFVDIALGWLANLPSVLEEVI 64
           F  +G+ +E+G EE  E LK L+ ELK   K+FF G+  GF DIA   +     V EE  
Sbjct: 111 FFRKGEEQEKGKEEVYEMLKVLDNELK--DKKFFAGDKFGFADIAANLVGFWLGVFEEGY 170

Query: 65  GLSLITQQTFPFLSKWTHEFANAPIINQHWPPKQQLV 102
           G  L+  + FP  SKW  E+ N   +N+  PP+ +L+
Sbjct: 171 GDVLVKSEKFPNFSKWRDEYINCSQVNESLPPRDELL 205

[Close]

BLAST of Carg06361 vs. ExPASy TrEMBL
Match: A0A6J1IGU2 (probable glutathione S-transferase OS=Cucurbita maxima OX=3661 GN=LOC111472760 PE=3 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 1.1e-48
Identity = 99/102 (97.06%), Postives = 100/102 (98.04%), Query Frame = 0


Query: 1   MKKVFMNEGKLKEEGIEEAKENLKHLEEELKAGGKRFFGGEVIGFVDIALGWLANLPSVL 60
           MKKVFM EGKLKEEGIEEAKENLKHLEEELKAGGKRFFGGEVIGFVD+ALGWLANLPSVL
Sbjct: 112 MKKVFMKEGKLKEEGIEEAKENLKHLEEELKAGGKRFFGGEVIGFVDVALGWLANLPSVL 171

Query: 61  EEVIGLSLITQQTFPFLSKWTHEFANAPIINQHWPPKQQLVT 103
           EEVIGLSLITQQTFP LSKWTHEFANAPIINQHWPPKQQLVT
Sbjct: 172 EEVIGLSLITQQTFPLLSKWTHEFANAPIINQHWPPKQQLVT 213

[Close]

BLAST of Carg06361 vs. ExPASy TrEMBL
Match: A0A6J1GEL0 (probable glutathione S-transferase OS=Cucurbita moschata OX=3662 GN=LOC111453233 PE=3 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 1.3e-47
Identity = 98/102 (96.08%), Postives = 100/102 (98.04%), Query Frame = 0


Query: 1   MKKVFMNEGKLKEEGIEEAKENLKHLEEELKAGGKRFFGGEVIGFVDIALGWLANLPSVL 60
           MK+VFMNEGKLKEEGIEEAKE+LKHLEEELKA GKRFFGGEVIGFVDIALGWLANLPSVL
Sbjct: 113 MKRVFMNEGKLKEEGIEEAKESLKHLEEELKARGKRFFGGEVIGFVDIALGWLANLPSVL 172

Query: 61  EEVIGLSLITQQTFPFLSKWTHEFANAPIINQHWPPKQQLVT 103
           EEVIGLSLITQQTFP LSKWTHEFANAPIINQHWPPKQQLVT
Sbjct: 173 EEVIGLSLITQQTFPLLSKWTHEFANAPIINQHWPPKQQLVT 214

[Close]

BLAST of Carg06361 vs. ExPASy TrEMBL
Match: A0A0A0L3V7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G126940 PE=3 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 1.4e-35
Identity = 75/102 (73.53%), Postives = 91/102 (89.22%), Query Frame = 0


Query: 1   MKKVFMNEGKLKEEGIEEAKENLKHLEEELKAGGKRFFGGEVIGFVDIALGWLANLPSVL 60
           +KKVFMN+GK++EEG+EEA ENLKHLEEELK  GKRFFGGE IGFVDIA+GWLAN+ SV+
Sbjct: 109 IKKVFMNQGKVREEGVEEAMENLKHLEEELK--GKRFFGGEAIGFVDIAVGWLANIVSVM 168

Query: 61  EEVIGLSLITQQTFPFLSKWTHEFANAPIINQHWPPKQQLVT 103
           EEV+GL LIT++ FP LSKWT EFA APIIN++WPP+ +L+T
Sbjct: 169 EEVVGLELITEERFPLLSKWTKEFAAAPIINENWPPRDKLIT 208

[Close]

BLAST of Carg06361 vs. ExPASy TrEMBL
Match: A0A5A7U184 (Putative glutathione S-transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G003640 PE=4 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 4.2e-35
Identity = 75/101 (74.26%), Postives = 90/101 (89.11%), Query Frame = 0


Query: 1   MKKVFMNEGKLKEEGIEEAKENLKHLEEELKAGGKRFFGGEVIGFVDIALGWLANLPSVL 60
           +KKVFMN+GK++EEG+EEA ENLKHLEEELK  GKRFFGGE IGFVDIA+GWLANL SV+
Sbjct: 330 IKKVFMNQGKVREEGVEEALENLKHLEEELK--GKRFFGGEAIGFVDIAVGWLANLVSVI 389

Query: 61  EEVIGLSLITQQTFPFLSKWTHEFANAPIINQHWPPKQQLV 102
           EEV+GL LIT++ FP LSKWT EFA APIIN++WPP+ +L+
Sbjct: 390 EEVVGLELITEERFPLLSKWTKEFAAAPIINENWPPRDKLI 428

[Close]

BLAST of Carg06361 vs. ExPASy TrEMBL
Match: A0A1S3AWH4 (probable glutathione S-transferase OS=Cucumis melo OX=3656 GN=LOC103483384 PE=3 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 4.2e-35
Identity = 75/101 (74.26%), Postives = 90/101 (89.11%), Query Frame = 0


Query: 1   MKKVFMNEGKLKEEGIEEAKENLKHLEEELKAGGKRFFGGEVIGFVDIALGWLANLPSVL 60
           +KKVFMN+GK++EEG+EEA ENLKHLEEELK  GKRFFGGE IGFVDIA+GWLANL SV+
Sbjct: 109 IKKVFMNQGKVREEGVEEALENLKHLEEELK--GKRFFGGEAIGFVDIAVGWLANLVSVI 168

Query: 61  EEVIGLSLITQQTFPFLSKWTHEFANAPIINQHWPPKQQLV 102
           EEV+GL LIT++ FP LSKWT EFA APIIN++WPP+ +L+
Sbjct: 169 EEVVGLELITEERFPLLSKWTKEFAAAPIINENWPPRDKLI 207

[Close]

BLAST of Carg06361 vs. TAIR 10
Match: AT3G09270.1 (glutathione S-transferase TAU 8 )

HSP 1 Score: 73.9 bits (180), Expect = 1.2e-13
Identity = 40/101 (39.60%), Postives = 61/101 (60.40%), Query Frame = 0


Query: 1   MKKVFMNEGKLKEEGIEEAKENLKHLEEELKAGGKRFFGGEVIGFVDIALGWLANLPSVL 60
           +KK        +E+ ++EA E LK LE+EL  G K FFGGE IGFVDIA  ++     + 
Sbjct: 113 VKKACWGPESEREKEVKEAYEGLKCLEKEL--GDKLFFGGETIGFVDIAADFIGYWLGIF 172

Query: 61  EEVIGLSLITQQTFPFLSKWTHEFANAPIINQHWPPKQQLV 102
           +E  G++++T + FP L +W+ +F     I +  PPK++LV
Sbjct: 173 QEASGVTIMTAEEFPKLQRWSEDFVGNNFIKEVLPPKEKLV 211

[Close]

BLAST of Carg06361 vs. TAIR 10
Match: AT2G29480.1 (glutathione S-transferase tau 2 )

HSP 1 Score: 57.4 bits (137), Expect = 1.1e-08
Identity = 33/92 (35.87%), Postives = 52/92 (56.52%), Query Frame = 0


Query: 16  IEEAKENLKHLEEELKAGGKRFFGGEVIGFVDIALGWLANLPSVLE---EVIGLSLITQQ 75
           IEE +E L  LE+E+   GK FFGG+ IGF+D+  G  + +P  L    E +G+ +  + 
Sbjct: 127 IEEIREMLMFLEKEVT--GKDFFGGKTIGFLDMVAG--SMIPFCLARAWECLGIDMTPED 186

Query: 76  TFPFLSKWTHEFANAPIINQHWPPKQQLVTNM 105
           TFP L++W        I+ +  PPK++ +  M
Sbjct: 187 TFPELNRWIKNLNEVEIVRECIPPKEKHIERM 214

[Close]

BLAST of Carg06361 vs. TAIR 10
Match: AT2G29450.1 (glutathione S-transferase tau 5 )

HSP 1 Score: 57.4 bits (137), Expect = 1.1e-08
Identity = 36/107 (33.64%), Postives = 59/107 (55.14%), Query Frame = 0


Query: 5   FMNEGKLKEEG----IEEAKENLKHLEEELKAGGKRFFGGEVIGFVDIALGWLANLPSVL 64
           F++  +  E+G     E+ +E + +LE+EL   GK +FGG+ +GF+D   G L  +P  L
Sbjct: 112 FISMARADEKGREVLAEQVRELIMYLEKELV--GKDYFGGKTVGFLDFVAGSL--IPFCL 171

Query: 65  E---EVIGLSLITQQTFPFLSKWTHEFANAPIINQHWPPKQQLVTNM 105
           E   E IGL +IT++ FP   +W        I+    PP+++ V +M
Sbjct: 172 ERGWEGIGLEVITEEKFPEFKRWVRNLEKVEIVKDCVPPREEHVEHM 214

[Close]

BLAST of Carg06361 vs. TAIR 10
Match: AT2G29490.1 (glutathione S-transferase TAU 1 )

HSP 1 Score: 55.8 bits (133), Expect = 3.3e-08
Identity = 34/99 (34.34%), Postives = 55/99 (55.56%), Query Frame = 0


Query: 10  KLKEEGIEEAKENLKHLEEELKAGGKRFFGGEVIGFVDIALGWLANLPSVLEEV---IGL 69
           K +E  IEE +E L  LE+E+   GK FFGG+ IGF+D+  G  + +P  L  +   IG+
Sbjct: 121 KGREVAIEETRELLMFLEKEVT--GKDFFGGKTIGFLDMIAG--SMIPFCLARLWKGIGI 180

Query: 70  SLITQQTFPFLSKWTHEFANAPIINQHWPPKQQLVTNMT 106
            +I ++ FP L++W         +    PP+++ +  MT
Sbjct: 181 DMIPEEKFPELNRWIKNLEEVEAVRGCIPPREKQIERMT 215

[Close]

BLAST of Carg06361 vs. TAIR 10
Match: AT2G29460.1 (glutathione S-transferase tau 4 )

HSP 1 Score: 55.1 bits (131), Expect = 5.6e-08
Identity = 35/106 (33.02%), Postives = 60/106 (56.60%), Query Frame = 0


Query: 5   FMNEGKLK---EEGIEEAKENLKHLEEELKAGGKRFFGGEVIGFVDIALGWLANLPSVLE 64
           FM+  K +   E  I+EA+E    LE+E+   GK FFGG+ IGF+D+  G  + +P  L 
Sbjct: 113 FMSVAKAEKGVEVAIKEAQELFMFLEKEVT--GKDFFGGKTIGFLDLVAG--SMIPFCLA 172

Query: 65  ---EVIGLSLITQQTFPFLSKWTHEFANAPIINQHWPPKQQLVTNM 105
              E +G+ +I ++ FP L++W        I+ +  PP+++ + +M
Sbjct: 173 RGWEGMGIDMIPEEKFPELNRWIKNLKEIEIVRECIPPREEQIEHM 214

The following BLAST results are available for this feature:

BLAST of Carg06361 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7028689.1	2.0e-76	100.00	putative glutathione S-transferase, partial [Cucurbita argyrosperma subsp. argyr...	[more]
KAG6597218.1	7.3e-50	100.00	hypothetical protein SDJN03_10398, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022974174.1	2.3e-48	97.06	probable glutathione S-transferase [Cucurbita maxima] >XP_022975554.1 probable g...	[more]
XP_023540933.1	3.1e-48	97.06	probable glutathione S-transferase [Cucurbita pepo subsp. pepo]	[more]
XP_022950014.1	2.6e-47	96.08	probable glutathione S-transferase [Cucurbita moschata]	[more]

BLAST of Carg06361 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P32110	1.8e-14	43.43	Probable glutathione S-transferase OS=Glycine max OX=3847 GN=HSP26-A PE=2 SV=1	[more]
Q9SR36	1.6e-12	39.60	Glutathione S-transferase U8 OS=Arabidopsis thaliana OX=3702 GN=GSTU8 PE=2 SV=1	[more]
Q9FQA3	1.1e-11	33.67	Glutathione transferase GST 23 OS=Zea mays OX=4577 PE=2 SV=1	[more]
Q03662	9.0e-11	39.39	Probable glutathione S-transferase OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
Q03663	1.2e-10	39.18	Probable glutathione S-transferase OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]

BLAST of Carg06361 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IGU2	1.1e-48	97.06	probable glutathione S-transferase OS=Cucurbita maxima OX=3661 GN=LOC111472760 P...	[more]
A0A6J1GEL0	1.3e-47	96.08	probable glutathione S-transferase OS=Cucurbita moschata OX=3662 GN=LOC111453233...	[more]
A0A0A0L3V7	1.4e-35	73.53	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G126940 PE=3 SV=1	[more]
A0A5A7U184	4.2e-35	74.26	Putative glutathione S-transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3AWH4	4.2e-35	74.26	probable glutathione S-transferase OS=Cucumis melo OX=3656 GN=LOC103483384 PE=3 ...	[more]

BLAST of Carg06361 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G09270.1	1.2e-13	39.60	glutathione S-transferase TAU 8	[more]
AT2G29480.1	1.1e-08	35.87	glutathione S-transferase tau 2	[more]
AT2G29450.1	1.1e-08	33.64	glutathione S-transferase tau 5	[more]
AT2G29490.1	3.3e-08	34.34	glutathione S-transferase TAU 1	[more]
AT2G29460.1	5.6e-08	33.02	glutathione S-transferase tau 4	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 9..36
None	No IPR available	GENE3D	1.20.1050.10		coord: 1..112 e-value: 6.1E-29 score: 102.7
None	No IPR available	PANTHER	PTHR11260:SF638	S-TRANSFERASE, PUTATIVE-RELATED	coord: 3..107
None	No IPR available	PANTHER	PTHR11260	GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, GST DOMAIN CONTAINING	coord: 3..107
IPR004046	Glutathione S-transferase, C-terminal	PFAM	PF00043	GST_C	coord: 10..88 e-value: 1.7E-7 score: 31.3
IPR010987	Glutathione S-transferase, C-terminal-like	PROSITE	PS50405	GST_CTER	coord: 1..104 score: 11.954026
IPR045074	Glutathione S-transferases Tau, C-terminal alpha helical domain, plant	CDD	cd03185	GST_C_Tau	coord: 1..101 e-value: 5.30031E-33 score: 111.116
IPR036282	Glutathione S-transferase, C-terminal domain superfamily	SUPERFAMILY	47616	GST C-terminal domain-like	coord: 4..102

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg06361-RA	Carg06361-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006749	glutathione metabolic process
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0140359	ABC-type transporter activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004364	glutathione transferase activity