Carg06360 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg06360
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Calcium-binding EF hand family protein
Location	Carg_Chr06: 7599771 .. 7604140 (+)
RNA-Seq Expression	Carg06360
Synteny	Carg06360

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATAACTTGCATTCCGGGAAAGGAAGGTCGCTTTATTCCAAGTTTCCTTCCGAACAGGAAAAGGAAATGTGTGGCAGAAGCAGTTTATGATTTTTTGTTTTTTTTTTTTCAGATACAAAACCTCAGGAAATGATAAATTTCGTTATGGTTGCTACTAAGGCGGGCATCTCGATCTTCCTTTACTCGAAGAACCGATTTCAATCACGGTTGGGGGCTGCTTTACGAGGTCTGTGATCTTCAATCGCCGTCGCTGCCGTCTAGTATGGGACAAATCTTGGGGAGATTTGGAGGTAAATTGACCTAACTTCACTTTTCGTATCCTTCGATTTTGAATTCGTTTTCAGTTTTCTGTTTGGATCCGTCGATAAATTGATTAATGGAACAAGCGAAATGGCGAATGCATAGTCACAGAGTAGATCATACCGAGATCGATCTTATTAGTGATATGCGATTAATCTGCAATTCTTCCTCGTTACTGATATTGTCTTAGACTTCAACCGATGAACTTACCGGTTTTGTTTCTTGTTTGGAGTGTTAGATTAGCTGATTTGTGCTGTCTCTGTGGATTTGATACCGTGTCTGCTTGCAATTGAGGGAGACAGACAGGATTTTTTATAAGATTAGAGACGGAGCGATGATTAATTAAAATATTCTTAAGCTTTTGTCCTTTCTGTTGCAGGAGAAGAATGGAGGAAGAAGAAGTTGAAGACGATATCAGACAGGATATTCGAGAAGATAAAAAAAAACAAATCAGCTGAGGAGGAGCTGTCATTTGAAGAATTATATATTGCCACGTTACTTGTGTTCAAGTAAGAAGCTCTATTATTTACTTCACATTTTTCCTGCTCCAATTTTTTTGTTGCTCATTCGTTTGTTTATTTATTTATTTACAACTTGGAGTGGGATATCACTCTTTATATTGTGTTCATGATCCATTGCTTTGTGAGAGAATTTGCAAAACCCATGACTGACGGACAGTAAGAATTGTAAGTTGGTTTGGCAAATTTCCATTCCTTATTTCTTGGTCCTCTAACTAAGAGAATAGAAATGTGTTTGGTAATAATTTCTTGTTCTTCCATATTGTAAATTTTTAGACAAAATTATCACATAAAAACGATTTGTTTCGAATGCTAGGGAAGACTGAAGAGGGTGGATTGTGAGACCCCACGTCGATTGGGGAGGAGAACGAAACATTCTTTATAAGGGTGTGGAAACCTTTCCCTAGCATACGCGTTGTAAAACCTTGAGAGGAAGTCCAAGAGGGAAAACCTAAAGAGGACACTATCTCTAGTAGTGGATTTGGGCTAGGGAAGACGCTGAAGAGGGTGGATTGTGAGATCCCACGTCGATTGGGAAGGAGAACGAAACATTATTTATAAGGGTGTGGAAACCTTTCCCCTAGCAGACGGGTTTTAAAACCTTGAGGGGAAGCCCATGAGGGAAAACCTAAAGAGGGCACTATCTCTAGTAGTGGATTTGGGCTAGGGAAGACGCTGAAGAGGGTGGATTGTGAGATCCCACGTCGATTGGGGAGGAGAACAAAATATTCTTTATAAGGGTGTGGAAACCTTTCCCTAGCAGACGCGTTTTAAAACTTTGAGAGGAAGCCCTGGAGGGAAAACCTAAAGAGGACACTATTTTTAGTAGTGAATTTGGGCTAGGGAAGACGCTGAAGAGGGTGGATTGTGAGATCCCACGTGGGGAGGAGAACGAACCATTATTTATAAGGGTGTGGAAACCTTTTCCTGGCAGACGCATTTTAAAATCTTGAGAGGAATCCCAGGAGGGAAAACCTAAAGAGGACACTATCTCTAGTAGTGGATTTGGGCTAGGGAAGACGCTGAAGAGGGTGGATTGTGAGATCCCATGTCGATTGGGGAGGAGAACGAAACATTGTTTATAAGGGTGTGAAAACCTTTCCTTTAGCATACGAGTTTTAAAACCTTGAGAGGAAGTCCAATAGGGAAAACCTAAAGAGGCACTATCTCTAGTAGTGGATTTGGGCTCGTAGATATAATGTTTATTATACATTTTACGAGTCAAAATAATAATCTGACGTTTTTTTATTTGTTCTTTTTGAAAAAATTGAGAAAAACTTGATCTTGTTGAAGAAATGAGAAACAGAAACACTTATCAATTGTCAATGTTCTTCAAAATCTGGTAATAGAAAACAGGAAACTAGAAAAATAAATGTGATTGAACAAGTCCTGAATTTTCAAACTGGGCTTGAACTTTGATCCTTGGAGATATCTCTTGAACATCTCAAGCTTTTGGTGATCTGATGAGGCATCTGTGAACTTGGCTTAACCTGAATAAAAACTTCTGATGCAGTGATATTAATAAGCTGTTCTCTGGCACACATCTTGATCCGCCTTCAAGGGAACATTTCAAACAACTCATAGCGGTACTCTGTTTATACAATTTATTACTCTGTTTATACAATTTATTACAAAGCTTTAACGATATGAAAAAGTGGCATCTGTTGATGATTGGGAAAACCTAAACTCGTTGGATATTAATGCCTAACCTTGTTTACTTTAGGTTTAATGCTCAGTAATTCTTGTTCTTTACTTGGTGCTAATGAACTTGGCTGAAGTATGGCTATCAACTTGCATGTGGTGCTGCTTGTTTTACTTTCCTATTCCTATTTTTGTCATGTTCGTCAATAATTTGTGGCTTTATTGAATTGTAGTAGCTTCCCTGCTCTTCCTGTTGCCATGTTCTTCAAAAAAAGTGGCCACTTTTGTTAACAATTTTGTTTTTATGCTTGTTTTCTTTCGGTTTCTTTACTGTGTTTTTCATCTTCCTTAGAGAAACTAGTCAAATTCCAAAACGAAAAGTGGGTTCTCTAAAACTACTTTTTTTCGGCCTGATTATTTGATTTGTTGGTGTTGAGTTTTTGAAAAGTAAGCTTATAGATCTGTAACCGCCCAAGCCCAAGCCTAAACCCACTGCCAATAGATATTGTCTGTTTTGGCCCCTTACGGTTTTTAAAACGCATTTGCTGGGGAGGAGTTTTCACACCCTTATAAAGAATGCTTCGTTCCCCTCTCCAACCAATGTGGAATCTCACGAGATCTTTACTTCTTTTGTGTCTTTTTTCTTTTTAATTTTCAAAATCCAAGTCAAGTTTTGAAAATGGAAAATAATAGTGTTTTTAAAACCATGTTTTTGTTTTTAGAATTAGACTAACAATTCAAATGTATCTTTTAAAAAGGGTTAAAATCATCGTAAATAGATAAGGAAACAAACATAAATTTTGTAAACCAAATAGTTATAGAATAGAACTTTAGTTTTAAGAACGTAAGTAAGTAGATAGCAAAAAAATAAGTAATTCAAAGCTGTATGAACTTTAAAAAATATATTTAAACACACTTCAAATTCTGGGATCAACTTAAGCATAAAATACCTGCTGATCATTAAGTGCCAACTTAAATTTCTGTGTTCATATACCTGCTGATCAAAAGCAAACATAAATTTCATGCTGATCAAACCAAATTTCTGTAGTACCTATAAGCATGCCAACTTCAGTTCATTAACTGCCCAACTTAGTGTTCATATAACCTGCTGATCAACTTAAGTAAGTAACCTGCTAATAACAAAAAATGCCAACTTCAAATTTCTGTGTTCATATACCTGCTGATCAACTTAAGCATGCCAACTTCAAATTTCTGTGTTCATATACCTGCTGATCAACTTAAGCATGCCAACTTCAAATTTCTGTGTTCATATACCTGCTGATCAACTTAAGCATGCCAACTTCAAATTTCTGTGTTCATATACCTGCTGATCAACTTAAGCATGCCAACTTCAAATTTCTGTGTTCATATACCTGCTGATCAACTTAAGCATGCCAACTTCAAATTTCTGTGTTCAATTGCTCTGTGCCAACTTCATTACCTGCTGATCAACTTAAGCATGCCAACTAAGCATATACCTGCTGATCAACTTAAGCATGCAAATTTCTGTGTTCATGTTCAACTTAAGCATGCCACTTCTTCTGTGCTGATCAACTTAAGCATGCTTGTGGCTCATTGTATTATATCAGGTACACGATACAAACAAGAACACAAAAATCGACAAAGGAGAATTTTTTGGTTTTATGGAGGAGTTGACATCTGAAACGTTCAAATCAGTGAGCCAGAAATTAGTCCTCACTTTGGTGATTGCACCAACGATCGCAGTGTTCACAAAGAAACGTACTGAAGGAGTTCCAGGCGTTGGAAAACTGGTGCAAAAACTCCCCTCTTCAGCATATGCTTCCATTATCACTCTTGCTGCTGTGCTGTTTCAGAGCTCTCAGCATCCGCTTCTTAAATGATAAGTTGAGACTCCTCTGTTTTCCTTCCTTTGTTCTTTTTTTTTTT

mRNA sequence

ATGGATAACTTGCATTCCGGGAAAGGAAGGTCGCTTTATTCCAAGTTTCCTTCCGAACAGGAAAAGGAAATGCGGGCATCTCGATCTTCCTTTACTCGAAGAACCGATTTCAATCACGGTTGGGGGCTGCTTTACGAGGTCTGTGATCTTCAATCGCCGTCGCTGCCGTCTAGTATGGGACAAATCTTGGGGAGATTTGGAGGAGAAGAATGGAGGAAGAAGAAGTTGAAGACGATATCAGACAGGATATTCGAGAAGATAAAAAAAAACAAATCAGCTGAGGAGGAGCTGTCATTTGAAGAATTATATATTGCCACGTTACTTGTGTTCAATGATATTAATAAGCTGTTCTCTGGCACACATCTTGATCCGCCTTCAAGGGAACATTTCAAACAACTCATAGCGGTACACGATACAAACAAGAACACAAAAATCGACAAAGGAGAATTTTTTGGTTTTATGGAGGAGTTGACATCTGAAACGTTCAAATCAGTGAGCCAGAAATTAGTCCTCACTTTGGTGATTGCACCAACGATCGCAGTGTTCACAAAGAAACGTACTGAAGGAGTTCCAGGCGTTGGAAAACTGGTGCAAAAACTCCCCTCTTCAGCATATGCTTCCATTATCACTCTTGCTGCTGTGCTGTTTCAGAGCTCTCAGCATCCGCTTCTTAAATGATAAGTTGAGACTCCTCTGTTTTCCTTCCTTTGTTCTTTTTTTTTTT

Coding sequence (CDS)

ATGGATAACTTGCATTCCGGGAAAGGAAGGTCGCTTTATTCCAAGTTTCCTTCCGAACAGGAAAAGGAAATGCGGGCATCTCGATCTTCCTTTACTCGAAGAACCGATTTCAATCACGGTTGGGGGCTGCTTTACGAGGTCTGTGATCTTCAATCGCCGTCGCTGCCGTCTAGTATGGGACAAATCTTGGGGAGATTTGGAGGAGAAGAATGGAGGAAGAAGAAGTTGAAGACGATATCAGACAGGATATTCGAGAAGATAAAAAAAAACAAATCAGCTGAGGAGGAGCTGTCATTTGAAGAATTATATATTGCCACGTTACTTGTGTTCAATGATATTAATAAGCTGTTCTCTGGCACACATCTTGATCCGCCTTCAAGGGAACATTTCAAACAACTCATAGCGGTACACGATACAAACAAGAACACAAAAATCGACAAAGGAGAATTTTTTGGTTTTATGGAGGAGTTGACATCTGAAACGTTCAAATCAGTGAGCCAGAAATTAGTCCTCACTTTGGTGATTGCACCAACGATCGCAGTGTTCACAAAGAAACGTACTGAAGGAGTTCCAGGCGTTGGAAAACTGGTGCAAAAACTCCCCTCTTCAGCATATGCTTCCATTATCACTCTTGCTGCTGTGCTGTTTCAGAGCTCTCAGCATCCGCTTCTTAAATGA

Protein sequence

MDNLHSGKGRSLYSKFPSEQEKEMRASRSSFTRRTDFNHGWGLLYEVCDLQSPSLPSSMGQILGRFGGEEWRKKKLKTISDRIFEKIKKNKSAEEELSFEELYIATLLVFNDINKLFSGTHLDPPSREHFKQLIAVHDTNKNTKIDKGEFFGFMEELTSETFKSVSQKLVLTLVIAPTIAVFTKKRTEGVPGVGKLVQKLPSSAYASIITLAAVLFQSSQHPLLK

Homology

[Close]

BLAST of Carg06360 vs. NCBI nr
Match: KAG7028690.1 (hypothetical protein SDJN02_09871, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 441.8 bits (1135), Expect = 3.5e-120
Identity = 225/225 (100.00%), Postives = 225/225 (100.00%), Query Frame = 0


Query: 1   MDNLHSGKGRSLYSKFPSEQEKEMRASRSSFTRRTDFNHGWGLLYEVCDLQSPSLPSSMG 60
           MDNLHSGKGRSLYSKFPSEQEKEMRASRSSFTRRTDFNHGWGLLYEVCDLQSPSLPSSMG
Sbjct: 1   MDNLHSGKGRSLYSKFPSEQEKEMRASRSSFTRRTDFNHGWGLLYEVCDLQSPSLPSSMG 60

Query: 61  QILGRFGGEEWRKKKLKTISDRIFEKIKKNKSAEEELSFEELYIATLLVFNDINKLFSGT 120
           QILGRFGGEEWRKKKLKTISDRIFEKIKKNKSAEEELSFEELYIATLLVFNDINKLFSGT
Sbjct: 61  QILGRFGGEEWRKKKLKTISDRIFEKIKKNKSAEEELSFEELYIATLLVFNDINKLFSGT 120

Query: 121 HLDPPSREHFKQLIAVHDTNKNTKIDKGEFFGFMEELTSETFKSVSQKLVLTLVIAPTIA 180
           HLDPPSREHFKQLIAVHDTNKNTKIDKGEFFGFMEELTSETFKSVSQKLVLTLVIAPTIA
Sbjct: 121 HLDPPSREHFKQLIAVHDTNKNTKIDKGEFFGFMEELTSETFKSVSQKLVLTLVIAPTIA 180

Query: 181 VFTKKRTEGVPGVGKLVQKLPSSAYASIITLAAVLFQSSQHPLLK 226
           VFTKKRTEGVPGVGKLVQKLPSSAYASIITLAAVLFQSSQHPLLK
Sbjct: 181 VFTKKRTEGVPGVGKLVQKLPSSAYASIITLAAVLFQSSQHPLLK 225

[Close]

BLAST of Carg06360 vs. NCBI nr
Match: KAG6597219.1 (hypothetical protein SDJN03_10399, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 363.2 bits (931), Expect = 1.6e-96
Identity = 203/244 (83.20%), Postives = 203/244 (83.20%), Query Frame = 0


Query: 1   MDNLHSGKGRSLYSKFPSEQEKEMRASRSS-------------------FTRRTDFNHGW 60
           MDNLHSGKGRSLYSKFPSEQEKEM   RS                    FTR   FNH  
Sbjct: 1   MDNLHSGKGRSLYSKFPSEQEKEM-CGRSGGHLDLPLLEELISITVGGCFTRSVIFNH-- 60

Query: 61  GLLYEVCDLQSPSLPSSMGQILGRFGGEEWRKKKLKTISDRIFEKIKKNKSAEEELSFEE 120
                 C L      SSMGQILGRFGGEEWRKKKLKTISDRIFEKIKKNKSAEEELSFEE
Sbjct: 61  ----RRCRL------SSMGQILGRFGGEEWRKKKLKTISDRIFEKIKKNKSAEEELSFEE 120

Query: 121 LYIATLLVFNDINKLFSGTHLDPPSREHFKQLIAVHDTNKNTKIDKGEFFGFMEELTSET 180
           LYIATLLVFNDINKLFSGTHLDPPSREHFKQLIAVHDTNKNTKIDKGEFFGFMEELTSET
Sbjct: 121 LYIATLLVFNDINKLFSGTHLDPPSREHFKQLIAVHDTNKNTKIDKGEFFGFMEELTSET 180

Query: 181 FKSVSQKLVLTLVIAPTIAVFTKKRTEGVPGVGKLVQKLPSSAYASIITLAAVLFQSSQH 226
           FKSVSQKLVLTLVIAPTIAVFTKKRTEGVPGVGKLVQKLPSSAYASIITLAAVLFQSSQH
Sbjct: 181 FKSVSQKLVLTLVIAPTIAVFTKKRTEGVPGVGKLVQKLPSSAYASIITLAAVLFQSSQH 231

[Close]

BLAST of Carg06360 vs. NCBI nr
Match: XP_022950463.1 (uncharacterized protein LOC111453554 [Cucurbita moschata])

HSP 1 Score: 322.8 bits (826), Expect = 2.4e-84
Identity = 167/167 (100.00%), Postives = 167/167 (100.00%), Query Frame = 0


Query: 59  MGQILGRFGGEEWRKKKLKTISDRIFEKIKKNKSAEEELSFEELYIATLLVFNDINKLFS 118
           MGQILGRFGGEEWRKKKLKTISDRIFEKIKKNKSAEEELSFEELYIATLLVFNDINKLFS
Sbjct: 1   MGQILGRFGGEEWRKKKLKTISDRIFEKIKKNKSAEEELSFEELYIATLLVFNDINKLFS 60

Query: 119 GTHLDPPSREHFKQLIAVHDTNKNTKIDKGEFFGFMEELTSETFKSVSQKLVLTLVIAPT 178
           GTHLDPPSREHFKQLIAVHDTNKNTKIDKGEFFGFMEELTSETFKSVSQKLVLTLVIAPT
Sbjct: 61  GTHLDPPSREHFKQLIAVHDTNKNTKIDKGEFFGFMEELTSETFKSVSQKLVLTLVIAPT 120

Query: 179 IAVFTKKRTEGVPGVGKLVQKLPSSAYASIITLAAVLFQSSQHPLLK 226
           IAVFTKKRTEGVPGVGKLVQKLPSSAYASIITLAAVLFQSSQHPLLK
Sbjct: 121 IAVFTKKRTEGVPGVGKLVQKLPSSAYASIITLAAVLFQSSQHPLLK 167

[Close]

BLAST of Carg06360 vs. NCBI nr
Match: XP_023540969.1 (uncharacterized protein LOC111801195 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 276.9 bits (707), Expect = 1.5e-70
Identity = 148/167 (88.62%), Postives = 154/167 (92.22%), Query Frame = 0


Query: 59  MGQILGRFGGEEWRKKKLKTISDRIFEKIKKNKSAEEELSFEELYIATLLVFNDINKLFS 118
           MGQILGRFGG+EWR KKLKTISDRIF+KIKK +S +EELSFEELYIATLLVFNDINKL S
Sbjct: 1   MGQILGRFGGKEWRIKKLKTISDRIFDKIKK-QSDKEELSFEELYIATLLVFNDINKLLS 60

Query: 119 GTHLDPPSREHFKQLIAVHDTNKNTKIDKGEFFGFMEELTSETFKSVSQKLVLTLVIAPT 178
           G+H DPPSREHFKQLI  HD NKN KID  EFFGFM ELTSETF+SVSQKLVLTLVIAPT
Sbjct: 61  GSHHDPPSREHFKQLIKEHDRNKNEKIDNEEFFGFMMELTSETFQSVSQKLVLTLVIAPT 120

Query: 179 IAVFTKKRTEGVPGVGKLVQKLPSSAYASIITLAAVLFQSSQHPLLK 226
           IAVFTKKRTEGVPGVGKLVQKLPSSAYASIITLAAVLFQSSQ PLLK
Sbjct: 121 IAVFTKKRTEGVPGVGKLVQKLPSSAYASIITLAAVLFQSSQQPLLK 166

[Close]

BLAST of Carg06360 vs. NCBI nr
Match: XP_022974167.1 (uncharacterized protein LOC111472754 [Cucurbita maxima] >XP_022974169.1 uncharacterized protein LOC111472754 [Cucurbita maxima])

HSP 1 Score: 270.8 bits (691), Expect = 1.1e-68
Identity = 143/167 (85.63%), Postives = 152/167 (91.02%), Query Frame = 0


Query: 59  MGQILGRFGGEEWRKKKLKTISDRIFEKIKKNKSAEEELSFEELYIATLLVFNDINKLFS 118
           MGQ+LGRFGG++WR KKLK IS+RIF+KIKK +S +EELSFEELYIATLLVFNDINKL S
Sbjct: 1   MGQVLGRFGGKDWRIKKLKMISNRIFDKIKK-QSDKEELSFEELYIATLLVFNDINKLLS 60

Query: 119 GTHLDPPSREHFKQLIAVHDTNKNTKIDKGEFFGFMEELTSETFKSVSQKLVLTLVIAPT 178
           G+H DPPSREHFKQLI  HD+NKN KID  EFFGFM ELTSETFKSVSQKLVLTLVIAPT
Sbjct: 61  GSHHDPPSREHFKQLITAHDSNKNEKIDNEEFFGFMMELTSETFKSVSQKLVLTLVIAPT 120

Query: 179 IAVFTKKRTEGVPGVGKLVQKLPSSAYASIITLAAVLFQSSQHPLLK 226
           IAVFTKKRTEG PGVGKLVQKLPSSAYASIITLAAVLFQSS  PLLK
Sbjct: 121 IAVFTKKRTEGFPGVGKLVQKLPSSAYASIITLAAVLFQSSHQPLLK 166

[Close]

BLAST of Carg06360 vs. ExPASy TrEMBL
Match: A0A6J1GEY5 (uncharacterized protein LOC111453554 OS=Cucurbita moschata OX=3662 GN=LOC111453554 PE=4 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 1.2e-84
Identity = 167/167 (100.00%), Postives = 167/167 (100.00%), Query Frame = 0


Query: 59  MGQILGRFGGEEWRKKKLKTISDRIFEKIKKNKSAEEELSFEELYIATLLVFNDINKLFS 118
           MGQILGRFGGEEWRKKKLKTISDRIFEKIKKNKSAEEELSFEELYIATLLVFNDINKLFS
Sbjct: 1   MGQILGRFGGEEWRKKKLKTISDRIFEKIKKNKSAEEELSFEELYIATLLVFNDINKLFS 60

Query: 119 GTHLDPPSREHFKQLIAVHDTNKNTKIDKGEFFGFMEELTSETFKSVSQKLVLTLVIAPT 178
           GTHLDPPSREHFKQLIAVHDTNKNTKIDKGEFFGFMEELTSETFKSVSQKLVLTLVIAPT
Sbjct: 61  GTHLDPPSREHFKQLIAVHDTNKNTKIDKGEFFGFMEELTSETFKSVSQKLVLTLVIAPT 120

Query: 179 IAVFTKKRTEGVPGVGKLVQKLPSSAYASIITLAAVLFQSSQHPLLK 226
           IAVFTKKRTEGVPGVGKLVQKLPSSAYASIITLAAVLFQSSQHPLLK
Sbjct: 121 IAVFTKKRTEGVPGVGKLVQKLPSSAYASIITLAAVLFQSSQHPLLK 167

[Close]

BLAST of Carg06360 vs. ExPASy TrEMBL
Match: A0A6J1IGT6 (uncharacterized protein LOC111472754 OS=Cucurbita maxima OX=3661 GN=LOC111472754 PE=4 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 5.2e-69
Identity = 143/167 (85.63%), Postives = 152/167 (91.02%), Query Frame = 0


Query: 59  MGQILGRFGGEEWRKKKLKTISDRIFEKIKKNKSAEEELSFEELYIATLLVFNDINKLFS 118
           MGQ+LGRFGG++WR KKLK IS+RIF+KIKK +S +EELSFEELYIATLLVFNDINKL S
Sbjct: 1   MGQVLGRFGGKDWRIKKLKMISNRIFDKIKK-QSDKEELSFEELYIATLLVFNDINKLLS 60

Query: 119 GTHLDPPSREHFKQLIAVHDTNKNTKIDKGEFFGFMEELTSETFKSVSQKLVLTLVIAPT 178
           G+H DPPSREHFKQLI  HD+NKN KID  EFFGFM ELTSETFKSVSQKLVLTLVIAPT
Sbjct: 61  GSHHDPPSREHFKQLITAHDSNKNEKIDNEEFFGFMMELTSETFKSVSQKLVLTLVIAPT 120

Query: 179 IAVFTKKRTEGVPGVGKLVQKLPSSAYASIITLAAVLFQSSQHPLLK 226
           IAVFTKKRTEG PGVGKLVQKLPSSAYASIITLAAVLFQSS  PLLK
Sbjct: 121 IAVFTKKRTEGFPGVGKLVQKLPSSAYASIITLAAVLFQSSHQPLLK 166

[Close]

BLAST of Carg06360 vs. ExPASy TrEMBL
Match: A0A6J1ITL1 (uncharacterized protein LOC111479803 OS=Cucurbita maxima OX=3661 GN=LOC111479803 PE=4 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 8.1e-46
Identity = 100/167 (59.88%), Postives = 131/167 (78.44%), Query Frame = 0


Query: 59  MGQILGRFGGEEWRKKKLKTISDRIFEKIKKNKSAEEELSFEELYIATLLVFNDINKLFS 118
           M + LGRFGG+EWR ++L+ ISDRIF+ I   K   ++LSFE+LYIATLLV+NDINK   
Sbjct: 1   MRKALGRFGGKEWRDEQLRNISDRIFDMIGDKK---DKLSFEDLYIATLLVYNDINKRLP 60

Query: 119 GTHLDPPSREHFKQLIAVHDTNKNTKIDKGEFFGFMEELTSETFKSVSQKLVLTLVIAPT 178
            +HLDPP+ E F+QL+  +D N++ KI + EF GF+  LTS+ F S+S +L+LTLVIAPT
Sbjct: 61  ISHLDPPTEERFRQLMKENDWNEDKKIGRDEFLGFILSLTSDAFISLSHRLILTLVIAPT 120

Query: 179 IAVFTKKRTEGVPGVGKLVQKLPSSAYASIITLAAVLFQSSQHPLLK 226
           +AV TK+ TEG+P VG LVQKLPSSAYA ++TLAA +FQ+SQ P+LK
Sbjct: 121 VAVVTKRSTEGIPVVGSLVQKLPSSAYAFLVTLAAFVFQNSQKPMLK 164

[Close]

BLAST of Carg06360 vs. ExPASy TrEMBL
Match: A0A6J1EC16 (uncharacterized protein LOC111431834 OS=Cucurbita moschata OX=3662 GN=LOC111431834 PE=4 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 1.1e-45
Identity = 99/167 (59.28%), Postives = 131/167 (78.44%), Query Frame = 0


Query: 59  MGQILGRFGGEEWRKKKLKTISDRIFEKIKKNKSAEEELSFEELYIATLLVFNDINKLFS 118
           M + LGRFGG+EWR ++L+ ISDRIF+ I   K   ++LSFE+LYIATLLV+NDINK   
Sbjct: 1   MRKALGRFGGKEWRDEQLRNISDRIFDMIGDKK---DKLSFEDLYIATLLVYNDINKRLP 60

Query: 119 GTHLDPPSREHFKQLIAVHDTNKNTKIDKGEFFGFMEELTSETFKSVSQKLVLTLVIAPT 178
            +HLDPP+ E F++LI  +D N++ KI + EF GF+  LTS+ F S+S +L+LTLVIAPT
Sbjct: 61  ISHLDPPTEERFRELIKENDWNQDKKIGRDEFLGFILSLTSDAFISLSHRLILTLVIAPT 120

Query: 179 IAVFTKKRTEGVPGVGKLVQKLPSSAYASIITLAAVLFQSSQHPLLK 226
           +AV TK+ TEG+P +G LVQKLPSSAYA ++TLAA +FQ+SQ P+LK
Sbjct: 121 VAVVTKRSTEGIPVIGSLVQKLPSSAYAFLVTLAAFVFQNSQKPMLK 164

[Close]

BLAST of Carg06360 vs. ExPASy TrEMBL
Match: A0A0A0L6I0 (EF-hand domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G126970 PE=4 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 1.8e-45
Identity = 97/167 (58.08%), Postives = 131/167 (78.44%), Query Frame = 0


Query: 59  MGQILGRFGGEEWRKKKLKTISDRIFEKIKKNKSAEEELSFEELYIATLLVFNDINKLFS 118
           MGQ   R GG+EWR  +L+ IS++IF++  K     E LSFE+LYIATLLVFNDINK   
Sbjct: 1   MGQAFRRLGGKEWRNTQLENISNKIFDRFGK-----ERLSFEDLYIATLLVFNDINKYLP 60

Query: 119 GTHLDPPSREHFKQLIAVHDTNKNTKIDKGEFFGFMEELTSETFKSVSQKLVLTLVIAPT 178
           G H+DPP ++  K+++ + D N+N  ID+ EF  F+++LTSE+F SVSQ+L++TLV+APT
Sbjct: 61  GQHVDPPKKDRVKEVMEMCDKNQNYAIDREEFLEFVKQLTSESFISVSQRLIITLVVAPT 120

Query: 179 IAVFTKKRTEGVPGVGKLVQKLPSSAYASIITLAAVLFQSSQHPLLK 226
           +AV TKK TEGVPG+GK+VQK+PSSAYA ++TLAA+LFQ+S+  LLK
Sbjct: 121 LAVVTKKSTEGVPGLGKMVQKVPSSAYALLVTLAALLFQNSRQQLLK 162

[Close]

BLAST of Carg06360 vs. TAIR 10
Match: AT1G64850.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 165.2 bits (417), Expect = 5.9e-41
Identity = 80/160 (50.00%), Postives = 116/160 (72.50%), Query Frame = 0


Query: 59  MGQILGRFGGEEWRKKKLKTISDRIFEKIKKNKSAEEELSFEELYIATLLVFNDINKLFS 118
           MGQ+  +  G+EWR K+++ I DR+F++ K  ++    L+FEELYIA LLV+NDINK   
Sbjct: 1   MGQVFNKLRGKEWRHKQVQAICDRVFDRFKM-QTGRANLTFEELYIAVLLVYNDINKRLP 60

Query: 119 GTHLDPPSREHFKQLIAVHDTNKNTKIDKGEFFGFMEELTSETFKSVSQKLVLTLVIAPT 178
           G H DPPS++    ++   D N + ++D+ EF  F+E++T+ETF  VSQ L++ L++APT
Sbjct: 61  GPHFDPPSKDLVNSIMTDCDINLDGELDREEFVKFIEQITAETFNVVSQGLIIALIVAPT 120

Query: 179 IAVFTKKRTEGVPGVGKLVQKLPSSAYASIITLAAVLFQS 219
           +A+ TKK TEGVPGVGK+VQKLP+S YAS++TLA +   S
Sbjct: 121 VAIATKKATEGVPGVGKVVQKLPTSIYASLVTLAVMWVNS 159

[Close]

BLAST of Carg06360 vs. TAIR 10
Match: AT4G37445.1 (BEST Arabidopsis thaliana protein match is: Calcium-binding EF hand family protein (TAIR:AT1G64850.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 48.9 bits (115), Expect = 6.2e-06
Identity = 29/118 (24.58%), Postives = 59/118 (50.00%), Query Frame = 0


Query: 98  SFEELYIATLLVFNDINKLFSGTHLDPPSREHFKQLIAVHDTNKNTKIDKGEFFGFMEEL 157
           +F++ ++A L +FN+ N    G H D P+ +  K   A     K+ +  K  F  FM + 
Sbjct: 36  NFDDFHVAVLDIFNNFNSALPGRHFDFPTPDQIKVCFARWKEAKDEEEKKKLFIEFMSKS 95

Query: 158 TSETFKSVSQKLVLTLVIAPTIAVFTKKRTEGVPGVGKLVQKLPSSAYASIITLAAVL 216
              +   +    ++T +++P  A+  K+  E VP + KL++ +P   +   +T+ A++
Sbjct: 96  VKPS--KLDDVTMITGIVSPPAAMAAKRAGENVPQL-KLIKLIPDVIFVPTVTILAIV 150

The following BLAST results are available for this feature:

BLAST of Carg06360 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7028690.1	3.5e-120	100.00	hypothetical protein SDJN02_09871, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6597219.1	1.6e-96	83.20	hypothetical protein SDJN03_10399, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022950463.1	2.4e-84	100.00	uncharacterized protein LOC111453554 [Cucurbita moschata]	[more]
XP_023540969.1	1.5e-70	88.62	uncharacterized protein LOC111801195 [Cucurbita pepo subsp. pepo]	[more]
XP_022974167.1	1.1e-68	85.63	uncharacterized protein LOC111472754 [Cucurbita maxima] >XP_022974169.1 uncharac...	[more]

BLAST of Carg06360 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg06360 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GEY5	1.2e-84	100.00	uncharacterized protein LOC111453554 OS=Cucurbita moschata OX=3662 GN=LOC1114535...	[more]
A0A6J1IGT6	5.2e-69	85.63	uncharacterized protein LOC111472754 OS=Cucurbita maxima OX=3661 GN=LOC111472754...	[more]
A0A6J1ITL1	8.1e-46	59.88	uncharacterized protein LOC111479803 OS=Cucurbita maxima OX=3661 GN=LOC111479803...	[more]
A0A6J1EC16	1.1e-45	59.28	uncharacterized protein LOC111431834 OS=Cucurbita moschata OX=3662 GN=LOC1114318...	[more]
A0A0A0L6I0	1.8e-45	58.08	EF-hand domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G126970 PE=...	[more]

BLAST of Carg06360 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G64850.1	5.9e-41	50.00	Calcium-binding EF hand family protein	[more]
AT4G37445.1	6.2e-06	24.58	BEST Arabidopsis thaliana protein match is: Calcium-binding EF hand family prote...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.10.238.10		coord: 11..179 e-value: 2.5E-6 score: 29.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..29
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 15..29
None	No IPR available	PANTHER	PTHR11639	S100 CALCIUM-BINDING PROTEIN	coord: 63..221
None	No IPR available	PANTHER	PTHR11639:SF133	CALCIUM-BINDING EF HAND PROTEIN	coord: 63..221
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 125..160 score: 10.914899
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 82..164

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg06360-RA	Carg06360-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0048306	calcium-dependent protein binding
molecular_function	GO:0005509	calcium ion binding