Carg06325 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg06325
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionProtein kinase domain-containing protein
LocationCarg_Chr06: 7823993 .. 7826032 (-)
RNA-Seq ExpressionCarg06325
SyntenyCarg06325
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TGTGTACAAGTATTACCAAACAAGTCTTTTCTCCACCATTGTCTTCTTTTGTCTCCTTGTTTCCCCATTCTCCCTTCTTCTTCTTCTCCTTCAGCTGTTGTTTCTTTCTGTTCTGTACTCGGAGTTTTTCAATCTGGCATCGAGAACGTTCCTATCATAGTTCTTGTGGTTTTTCCTCTGTTTTTGCTTTTGTATCAATCTCTATAAGATGTGTTTTCAGCTGTTCTTTTGAGACCAGAAACAACTGAAAGCTTTTGCATTAAATTTCTTCTGGGGATAAATTGCAGAGGCTCAAAACAACGTGGGAATTGCCAAATTTCCAGATCTAATAGAAAATGAAGTGCTTTTTCTACTTCAAAAACAAGTCCAGGAGTAGGAGGCATCAAAGATCCGCACCTGAATTGAAAGGGGAGCTAGAATCTGACTTTTCGGGATGCAGTCAGACGGCTGCTTCTTCGTGCTCTCTAACATCGACTCGGAGTGTACCTGAATTGTATGAAGAGAAAGCACATAATTTGAGAGTTTTCTCATTTGCAGAGATGAGAGAGGCAACACATGACTTCAACAGGCTTCTTAAGATTGGACAAGGGGGATTTGGGAGTGTCTTTAAAGGCTCAATTAAGCCCATTGATGGGAAGGGAGATCCCCTTGTTGTTGCTATCAAGCAACTGAGTAAGGACGGCTTACAGGTATGTTTTTCTTTGAATTTATTGGCCGAATTTCTTCATTGTTGGAGATTCTGATTGGGTGATCTGGTTATATTCATTCCATATAATATATATGCATCAAGAACAGAGAATCATTACTACTATTCGTTAAGCTTTAATCCTATGGAAAATAAGGCAAGTTTTCATGAGGAAGTGATTTTTCTTTGTACGTACCATTTCTATTTTGCAAATAGGGCCACAAGCAGTGGCTGGCAGAAGTTCAATTTCTTGGTATAGTGGAGCATCCAAATCTTGTCCAACTCGTAGGATACTGTGCTGTGGATGGAACAAGAGGAATCCAAAGACTGCTCGTATACGAGTATATGACGAACAGAAGCTTGGAAGATCATCTCTTCAACAAGGCGCTACCGACACTCGCTTGGAAAACAAGATTGCAGATAGTACTTGGAGCAGCTCAGGGGTTGGCTTATCTGCATGAAGGATTAGAAGTTCAGGTATCATATCTTCTCAAATAGAATTCAATTTCAGGGCGTGTGCATTTAAACAAGATAGTTATACACAAACATACCACTTTTTGCTGTGACTAGATTTACCCTCATGTTCTTCTATAATGTGGCACAGATAATATACAGAGATTTTAAGAGTTCAAATGTGCTACTGGATGAAAATTTTCATCCAAAACTTTCAGATTTTGGGCTTGCCAGGGAAGGGCCAGAATTTGGGCGCACTCATGTTTCAACAGCAGTAAGTGTTTGTTTGTTTTTTTTGTTCTTCTCCCTTTAATCACTTACAGATGCTCACAGTGAACTACATCATGTACACTCAGGTGATGGGGACTAATGGATATGCAGCTCCGGACTACATCAAGACAGGCCATCTGACAGCGAAAAGTGATGTATGGAGCTATGGTGTTGTGCTTTATGAGATTTTAACTGGGAGACGATCATTGGAGAGACATCATCCAAGACCAGAACAGAATCTTGTGGAATGGGTTAAATATATCAATCCAGACAGCGAAAAGTTTAGTTCGATAATAGATCCTCGGCTAGAAAACGAGTACCCCATCAATACAGCTAGAAAAGTAGCAAAGTTAGCAAACACTTGCTTGGAGAAAAATGCGAAGGATAGGCCCTCAATGGCTGAAGTAGTAAACAGATTGAAGCAGATCATTAAAGCTGAGGACGACAATGAGCCTTACGAGAAAAGTCCTGATAACAGCGACAACAAACCCGACATGGAAAGAGAACCAGAGAAAACGAAGGTGATGGACTCGTGGAGAAGGCGGATGAGCCACCTGCAGAAACTGGGAGAGCATGTGGAGGGTGCAAGTAGAAGAAGATTTATGATAATGCAAAGAGCAAAAGTGCCTTAA

mRNA sequence

TGTGTACAAGTATTACCAAACAAGTCTTTTCTCCACCATTGTCTTCTTTTGTCTCCTTGTTTCCCCATTCTCCCTTCTTCTTCTTCTCCTTCAGCTGTTGTTTCTTTCTGTTCTGTACTCGGAGTTTTTCAATCTGGCATCGAGAACGTTCCTATCATAGTTCTTGTGGTTTTTCCTCTGTTTTTGCTTTTGTATCAATCTCTATAAGATGTGTTTTCAGCTGTTCTTTTGAGACCAGAAACAACTGAAAGCTTTTGCATTAAATTTCTTCTGGGGATAAATTGCAGAGGCTCAAAACAACGTGGGAATTGCCAAATTTCCAGATCTAATAGAAAATGAAGTGCTTTTTCTACTTCAAAAACAAGTCCAGGAGTAGGAGGCATCAAAGATCCGCACCTGAATTGAAAGGGGAGCTAGAATCTGACTTTTCGGGATGCAGTCAGACGGCTGCTTCTTCGTGCTCTCTAACATCGACTCGGAGTGTACCTGAATTGTATGAAGAGAAAGCACATAATTTGAGAGTTTTCTCATTTGCAGAGATGAGAGAGGCAACACATGACTTCAACAGGCTTCTTAAGATTGGACAAGGGGGATTTGGGAGTGTCTTTAAAGGCTCAATTAAGCCCATTGATGGGAAGGGAGATCCCCTTGTTGTTGCTATCAAGCAACTGAGTAAGGACGGCTTACAGGGCCACAAGCAGTGGCTGGCAGAAGTTCAATTTCTTGGTATAGTGGAGCATCCAAATCTTGTCCAACTCGTAGGATACTGTGCTGTGGATGGAACAAGAGGAATCCAAAGACTGCTCGTATACGAGTATATGACGAACAGAAGCTTGGAAGATCATCTCTTCAACAAGGCGCTACCGACACTCGCTTGGAAAACAAGATTGCAGATAGTACTTGGAGCAGCTCAGGGGTTGGCTTATCTGCATGAAGGATTAGAAGTTCAGATAATATACAGAGATTTTAAGAGTTCAAATGTGCTACTGGATGAAAATTTTCATCCAAAACTTTCAGATTTTGGGCTTGCCAGGGAAGGGCCAGAATTTGGGCGCACTCATGTTTCAACAGCAGTGATGGGGACTAATGGATATGCAGCTCCGGACTACATCAAGACAGGCCATCTGACAGCGAAAAGTGATGTATGGAGCTATGGTGTTGTGCTTTATGAGATTTTAACTGGGAGACGATCATTGGAGAGACATCATCCAAGACCAGAACAGAATCTTGTGGAATGGGTTAAATATATCAATCCAGACAGCGAAAAGTTTAGTTCGATAATAGATCCTCGGCTAGAAAACGAGTACCCCATCAATACAGCTAGAAAAGTAGCAAAGTTAGCAAACACTTGCTTGGAGAAAAATGCGAAGGATAGGCCCTCAATGGCTGAAGTAGTAAACAGATTGAAGCAGATCATTAAAGCTGAGGACGACAATGAGCCTTACGAGAAAAGTCCTGATAACAGCGACAACAAACCCGACATGGAAAGAGAACCAGAGAAAACGAAGGTGATGGACTCGTGGAGAAGGCGGATGAGCCACCTGCAGAAACTGGGAGAGCATGTGGAGGGTGCAAGTAGAAGAAGATTTATGATAATGCAAAGAGCAAAAGTGCCTTAA

Coding sequence (CDS)

ATGAAGTGCTTTTTCTACTTCAAAAACAAGTCCAGGAGTAGGAGGCATCAAAGATCCGCACCTGAATTGAAAGGGGAGCTAGAATCTGACTTTTCGGGATGCAGTCAGACGGCTGCTTCTTCGTGCTCTCTAACATCGACTCGGAGTGTACCTGAATTGTATGAAGAGAAAGCACATAATTTGAGAGTTTTCTCATTTGCAGAGATGAGAGAGGCAACACATGACTTCAACAGGCTTCTTAAGATTGGACAAGGGGGATTTGGGAGTGTCTTTAAAGGCTCAATTAAGCCCATTGATGGGAAGGGAGATCCCCTTGTTGTTGCTATCAAGCAACTGAGTAAGGACGGCTTACAGGGCCACAAGCAGTGGCTGGCAGAAGTTCAATTTCTTGGTATAGTGGAGCATCCAAATCTTGTCCAACTCGTAGGATACTGTGCTGTGGATGGAACAAGAGGAATCCAAAGACTGCTCGTATACGAGTATATGACGAACAGAAGCTTGGAAGATCATCTCTTCAACAAGGCGCTACCGACACTCGCTTGGAAAACAAGATTGCAGATAGTACTTGGAGCAGCTCAGGGGTTGGCTTATCTGCATGAAGGATTAGAAGTTCAGATAATATACAGAGATTTTAAGAGTTCAAATGTGCTACTGGATGAAAATTTTCATCCAAAACTTTCAGATTTTGGGCTTGCCAGGGAAGGGCCAGAATTTGGGCGCACTCATGTTTCAACAGCAGTGATGGGGACTAATGGATATGCAGCTCCGGACTACATCAAGACAGGCCATCTGACAGCGAAAAGTGATGTATGGAGCTATGGTGTTGTGCTTTATGAGATTTTAACTGGGAGACGATCATTGGAGAGACATCATCCAAGACCAGAACAGAATCTTGTGGAATGGGTTAAATATATCAATCCAGACAGCGAAAAGTTTAGTTCGATAATAGATCCTCGGCTAGAAAACGAGTACCCCATCAATACAGCTAGAAAAGTAGCAAAGTTAGCAAACACTTGCTTGGAGAAAAATGCGAAGGATAGGCCCTCAATGGCTGAAGTAGTAAACAGATTGAAGCAGATCATTAAAGCTGAGGACGACAATGAGCCTTACGAGAAAAGTCCTGATAACAGCGACAACAAACCCGACATGGAAAGAGAACCAGAGAAAACGAAGGTGATGGACTCGTGGAGAAGGCGGATGAGCCACCTGCAGAAACTGGGAGAGCATGTGGAGGGTGCAAGTAGAAGAAGATTTATGATAATGCAAAGAGCAAAAGTGCCTTAA

Protein sequence

MKCFFYFKNKSRSRRHQRSAPELKGELESDFSGCSQTAASSCSLTSTRSVPELYEEKAHNLRVFSFAEMREATHDFNRLLKIGQGGFGSVFKGSIKPIDGKGDPLVVAIKQLSKDGLQGHKQWLAEVQFLGIVEHPNLVQLVGYCAVDGTRGIQRLLVYEYMTNRSLEDHLFNKALPTLAWKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEFGRTHVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSYGVVLYEILTGRRSLERHHPRPEQNLVEWVKYINPDSEKFSSIIDPRLENEYPINTARKVAKLANTCLEKNAKDRPSMAEVVNRLKQIIKAEDDNEPYEKSPDNSDNKPDMEREPEKTKVMDSWRRRMSHLQKLGEHVEGASRRRFMIMQRAKVP
Homology
BLAST of Carg06325 vs. NCBI nr
Match: KAG7028725.1 (putative serine/threonine-protein kinase PBL19 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 857.1 bits (2213), Expect = 6.7e-245
Identity = 427/427 (100.00%), Postives = 427/427 (100.00%), Query Frame = 0

Query: 1   MKCFFYFKNKSRSRRHQRSAPELKGELESDFSGCSQTAASSCSLTSTRSVPELYEEKAHN 60
           MKCFFYFKNKSRSRRHQRSAPELKGELESDFSGCSQTAASSCSLTSTRSVPELYEEKAHN
Sbjct: 1   MKCFFYFKNKSRSRRHQRSAPELKGELESDFSGCSQTAASSCSLTSTRSVPELYEEKAHN 60

Query: 61  LRVFSFAEMREATHDFNRLLKIGQGGFGSVFKGSIKPIDGKGDPLVVAIKQLSKDGLQGH 120
           LRVFSFAEMREATHDFNRLLKIGQGGFGSVFKGSIKPIDGKGDPLVVAIKQLSKDGLQGH
Sbjct: 61  LRVFSFAEMREATHDFNRLLKIGQGGFGSVFKGSIKPIDGKGDPLVVAIKQLSKDGLQGH 120

Query: 121 KQWLAEVQFLGIVEHPNLVQLVGYCAVDGTRGIQRLLVYEYMTNRSLEDHLFNKALPTLA 180
           KQWLAEVQFLGIVEHPNLVQLVGYCAVDGTRGIQRLLVYEYMTNRSLEDHLFNKALPTLA
Sbjct: 121 KQWLAEVQFLGIVEHPNLVQLVGYCAVDGTRGIQRLLVYEYMTNRSLEDHLFNKALPTLA 180

Query: 181 WKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEFGR 240
           WKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEFGR
Sbjct: 181 WKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEFGR 240

Query: 241 THVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSYGVVLYEILTGRRSLERHHPRPEQNLVE 300
           THVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSYGVVLYEILTGRRSLERHHPRPEQNLVE
Sbjct: 241 THVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSYGVVLYEILTGRRSLERHHPRPEQNLVE 300

Query: 301 WVKYINPDSEKFSSIIDPRLENEYPINTARKVAKLANTCLEKNAKDRPSMAEVVNRLKQI 360
           WVKYINPDSEKFSSIIDPRLENEYPINTARKVAKLANTCLEKNAKDRPSMAEVVNRLKQI
Sbjct: 301 WVKYINPDSEKFSSIIDPRLENEYPINTARKVAKLANTCLEKNAKDRPSMAEVVNRLKQI 360

Query: 361 IKAEDDNEPYEKSPDNSDNKPDMEREPEKTKVMDSWRRRMSHLQKLGEHVEGASRRRFMI 420
           IKAEDDNEPYEKSPDNSDNKPDMEREPEKTKVMDSWRRRMSHLQKLGEHVEGASRRRFMI
Sbjct: 361 IKAEDDNEPYEKSPDNSDNKPDMEREPEKTKVMDSWRRRMSHLQKLGEHVEGASRRRFMI 420

Query: 421 MQRAKVP 428
           MQRAKVP
Sbjct: 421 MQRAKVP 427

BLAST of Carg06325 vs. NCBI nr
Match: XP_022940922.1 (probable serine/threonine-protein kinase PBL19 [Cucurbita moschata])

HSP 1 Score: 853.6 bits (2204), Expect = 7.4e-244
Identity = 425/427 (99.53%), Postives = 427/427 (100.00%), Query Frame = 0

Query: 1   MKCFFYFKNKSRSRRHQRSAPELKGELESDFSGCSQTAASSCSLTSTRSVPELYEEKAHN 60
           MKCFFYFKNKSRSRRHQRSAPELKGELES+FSGCSQTAASSCSLTSTRSVPELYEEKAHN
Sbjct: 1   MKCFFYFKNKSRSRRHQRSAPELKGELESNFSGCSQTAASSCSLTSTRSVPELYEEKAHN 60

Query: 61  LRVFSFAEMREATHDFNRLLKIGQGGFGSVFKGSIKPIDGKGDPLVVAIKQLSKDGLQGH 120
           LRVFSFAEMREATHDFNRLLKIGQGGFGSVFKGSIKPIDGKGDPLVVAIKQLSKDGLQGH
Sbjct: 61  LRVFSFAEMREATHDFNRLLKIGQGGFGSVFKGSIKPIDGKGDPLVVAIKQLSKDGLQGH 120

Query: 121 KQWLAEVQFLGIVEHPNLVQLVGYCAVDGTRGIQRLLVYEYMTNRSLEDHLFNKALPTLA 180
           KQWLAEVQFLGIVEHPNLVQLVGYCAVDGTRGIQRLLVYEYMTNRSLEDHLFNKALPTLA
Sbjct: 121 KQWLAEVQFLGIVEHPNLVQLVGYCAVDGTRGIQRLLVYEYMTNRSLEDHLFNKALPTLA 180

Query: 181 WKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEFGR 240
           WKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEFGR
Sbjct: 181 WKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEFGR 240

Query: 241 THVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSYGVVLYEILTGRRSLERHHPRPEQNLVE 300
           THVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSYGVVLYEILTGRRSLERHHPRPEQNLVE
Sbjct: 241 THVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSYGVVLYEILTGRRSLERHHPRPEQNLVE 300

Query: 301 WVKYINPDSEKFSSIIDPRLENEYPINTARKVAKLANTCLEKNAKDRPSMAEVVNRLKQI 360
           WVKYINPDSEKFSSIIDPRLENEYPINTARKVAKLANTCLEKNAKDRPSMAEVVNRLKQI
Sbjct: 301 WVKYINPDSEKFSSIIDPRLENEYPINTARKVAKLANTCLEKNAKDRPSMAEVVNRLKQI 360

Query: 361 IKAEDDNEPYEKSPDNSDNKPDMEREPEKTKVMDSWRRRMSHLQKLGEHVEGASRRRFMI 420
           IKA+DDNEPYEKSPDNSDNKPDMEREPEKTKVMDSWRRRMSHLQKLGEHVEGASRRRFMI
Sbjct: 361 IKAKDDNEPYEKSPDNSDNKPDMEREPEKTKVMDSWRRRMSHLQKLGEHVEGASRRRFMI 420

Query: 421 MQRAKVP 428
           MQRAKVP
Sbjct: 421 MQRAKVP 427

BLAST of Carg06325 vs. NCBI nr
Match: XP_023540606.1 (probable serine/threonine-protein kinase PBL19 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 850.5 bits (2196), Expect = 6.2e-243
Identity = 423/427 (99.06%), Postives = 426/427 (99.77%), Query Frame = 0

Query: 1   MKCFFYFKNKSRSRRHQRSAPELKGELESDFSGCSQTAASSCSLTSTRSVPELYEEKAHN 60
           MKCFFYFKNKSRSRRHQRSAPELKGELESDFSGCS+TAASSCSLTSTRSVP+LYEEKAHN
Sbjct: 1   MKCFFYFKNKSRSRRHQRSAPELKGELESDFSGCSRTAASSCSLTSTRSVPKLYEEKAHN 60

Query: 61  LRVFSFAEMREATHDFNRLLKIGQGGFGSVFKGSIKPIDGKGDPLVVAIKQLSKDGLQGH 120
           LRVFSFAEMREATHDFNRLLKIGQGGFGSVFKGSIKPIDGKGDPLVVAIKQLSKDGLQGH
Sbjct: 61  LRVFSFAEMREATHDFNRLLKIGQGGFGSVFKGSIKPIDGKGDPLVVAIKQLSKDGLQGH 120

Query: 121 KQWLAEVQFLGIVEHPNLVQLVGYCAVDGTRGIQRLLVYEYMTNRSLEDHLFNKALPTLA 180
           KQWLAEVQFLGIVEHPNLVQLVGYCAVDGTRGIQRLLVYEYMTNRSLEDHLFNKALPTLA
Sbjct: 121 KQWLAEVQFLGIVEHPNLVQLVGYCAVDGTRGIQRLLVYEYMTNRSLEDHLFNKALPTLA 180

Query: 181 WKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEFGR 240
           WKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEFGR
Sbjct: 181 WKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEFGR 240

Query: 241 THVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSYGVVLYEILTGRRSLERHHPRPEQNLVE 300
           THVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSYGVVLYEILTGRRSLERHHPRPEQNLVE
Sbjct: 241 THVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSYGVVLYEILTGRRSLERHHPRPEQNLVE 300

Query: 301 WVKYINPDSEKFSSIIDPRLENEYPINTARKVAKLANTCLEKNAKDRPSMAEVVNRLKQI 360
           WVKYINPDSEKFSSIIDPRLEN+YPINTARKVAKLANTCLEKNAKDRPSMAEVVNRLKQI
Sbjct: 301 WVKYINPDSEKFSSIIDPRLENQYPINTARKVAKLANTCLEKNAKDRPSMAEVVNRLKQI 360

Query: 361 IKAEDDNEPYEKSPDNSDNKPDMEREPEKTKVMDSWRRRMSHLQKLGEHVEGASRRRFMI 420
           IKAEDDNEPYEKSPDN DNKPDMEREPEKTKVMDSWRRRMSHLQKLGEHVEGASRRRFMI
Sbjct: 361 IKAEDDNEPYEKSPDNIDNKPDMEREPEKTKVMDSWRRRMSHLQKLGEHVEGASRRRFMI 420

Query: 421 MQRAKVP 428
           MQRAKVP
Sbjct: 421 MQRAKVP 427

BLAST of Carg06325 vs. NCBI nr
Match: XP_022974762.1 (probable serine/threonine-protein kinase PBL19 isoform X2 [Cucurbita maxima])

HSP 1 Score: 849.0 bits (2192), Expect = 1.8e-242
Identity = 421/427 (98.59%), Postives = 427/427 (100.00%), Query Frame = 0

Query: 1   MKCFFYFKNKSRSRRHQRSAPELKGELESDFSGCSQTAASSCSLTSTRSVPELYEEKAHN 60
           MKCFFYF+NKSRSRRHQRSAPELKGELESDFSGCS+TAASSCSLTSTRSVPELYEEKAHN
Sbjct: 1   MKCFFYFRNKSRSRRHQRSAPELKGELESDFSGCSRTAASSCSLTSTRSVPELYEEKAHN 60

Query: 61  LRVFSFAEMREATHDFNRLLKIGQGGFGSVFKGSIKPIDGKGDPLVVAIKQLSKDGLQGH 120
           LRVFSFAEMREATHDFNRLLKIGQGGFGSVFKGSIKPIDGKGDPLVVAIKQLSKDGLQGH
Sbjct: 61  LRVFSFAEMREATHDFNRLLKIGQGGFGSVFKGSIKPIDGKGDPLVVAIKQLSKDGLQGH 120

Query: 121 KQWLAEVQFLGIVEHPNLVQLVGYCAVDGTRGIQRLLVYEYMTNRSLEDHLFNKALPTLA 180
           KQWLAEVQFLGIVEHPNLVQLVGYCAVDGTRGIQRLL+YEY+TNRSLEDHLFNKALPTLA
Sbjct: 121 KQWLAEVQFLGIVEHPNLVQLVGYCAVDGTRGIQRLLIYEYVTNRSLEDHLFNKALPTLA 180

Query: 181 WKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEFGR 240
           WKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEFGR
Sbjct: 181 WKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEFGR 240

Query: 241 THVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSYGVVLYEILTGRRSLERHHPRPEQNLVE 300
           THVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSYGVVLYEILTGRRSLERHHPRPEQNLVE
Sbjct: 241 THVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSYGVVLYEILTGRRSLERHHPRPEQNLVE 300

Query: 301 WVKYINPDSEKFSSIIDPRLENEYPINTARKVAKLANTCLEKNAKDRPSMAEVVNRLKQI 360
           WVKYINPDS+KFSSIIDPRLENEYPINTARKVAKLANTCLEKNAKDRPSMAEVVNRLKQI
Sbjct: 301 WVKYINPDSKKFSSIIDPRLENEYPINTARKVAKLANTCLEKNAKDRPSMAEVVNRLKQI 360

Query: 361 IKAEDDNEPYEKSPDNSDNKPDMEREPEKTKVMDSWRRRMSHLQKLGEHVEGASRRRFMI 420
           IKAEDD+EPYEKSPDNSDNKPDMEREPEKTKVMDSWRRRMSHLQKLGEHVEGASRRRFMI
Sbjct: 361 IKAEDDDEPYEKSPDNSDNKPDMEREPEKTKVMDSWRRRMSHLQKLGEHVEGASRRRFMI 420

Query: 421 MQRAKVP 428
           MQRAKVP
Sbjct: 421 MQRAKVP 427

BLAST of Carg06325 vs. NCBI nr
Match: XP_023540605.1 (probable serine/threonine-protein kinase PBL19 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 835.9 bits (2158), Expect = 1.6e-238
Identity = 423/454 (93.17%), Postives = 426/454 (93.83%), Query Frame = 0

Query: 1   MKCFFYFKNKSRSRRHQRSAPELKGELESDFSGCSQTAASSCSLTSTRSVPELYEEKAHN 60
           MKCFFYFKNKSRSRRHQRSAPELKGELESDFSGCS+TAASSCSLTSTRSVP+LYEEKAHN
Sbjct: 1   MKCFFYFKNKSRSRRHQRSAPELKGELESDFSGCSRTAASSCSLTSTRSVPKLYEEKAHN 60

Query: 61  LRVFSFAEMREATHDFNRLLKIGQGGFGSVFKGSIKPIDGKGDPLVVAIKQLSKDGLQGH 120
           LRVFSFAEMREATHDFNRLLKIGQGGFGSVFKGSIKPIDGKGDPLVVAIKQLSKDGLQGH
Sbjct: 61  LRVFSFAEMREATHDFNRLLKIGQGGFGSVFKGSIKPIDGKGDPLVVAIKQLSKDGLQGH 120

Query: 121 KQWLAEVQFLGIVEHPNLVQLVGYCAVDGTRGIQRLLVYEYMTNRSLEDHLFNKALPTLA 180
           KQWLAEVQFLGIVEHPNLVQLVGYCAVDGTRGIQRLLVYEYMTNRSLEDHLFNKALPTLA
Sbjct: 121 KQWLAEVQFLGIVEHPNLVQLVGYCAVDGTRGIQRLLVYEYMTNRSLEDHLFNKALPTLA 180

Query: 181 WKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEFGR 240
           WKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEFGR
Sbjct: 181 WKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEFGR 240

Query: 241 THVSTA---------------------------VMGTNGYAAPDYIKTGHLTAKSDVWSY 300
           THVSTA                           VMGTNGYAAPDYIKTGHLTAKSDVWSY
Sbjct: 241 THVSTAVSVCLFFCSSPFNHLQMLTMNYIMNTQVMGTNGYAAPDYIKTGHLTAKSDVWSY 300

Query: 301 GVVLYEILTGRRSLERHHPRPEQNLVEWVKYINPDSEKFSSIIDPRLENEYPINTARKVA 360
           GVVLYEILTGRRSLERHHPRPEQNLVEWVKYINPDSEKFSSIIDPRLEN+YPINTARKVA
Sbjct: 301 GVVLYEILTGRRSLERHHPRPEQNLVEWVKYINPDSEKFSSIIDPRLENQYPINTARKVA 360

Query: 361 KLANTCLEKNAKDRPSMAEVVNRLKQIIKAEDDNEPYEKSPDNSDNKPDMEREPEKTKVM 420
           KLANTCLEKNAKDRPSMAEVVNRLKQIIKAEDDNEPYEKSPDN DNKPDMEREPEKTKVM
Sbjct: 361 KLANTCLEKNAKDRPSMAEVVNRLKQIIKAEDDNEPYEKSPDNIDNKPDMEREPEKTKVM 420

Query: 421 DSWRRRMSHLQKLGEHVEGASRRRFMIMQRAKVP 428
           DSWRRRMSHLQKLGEHVEGASRRRFMIMQRAKVP
Sbjct: 421 DSWRRRMSHLQKLGEHVEGASRRRFMIMQRAKVP 454

BLAST of Carg06325 vs. ExPASy Swiss-Prot
Match: Q9LTC0 (Probable serine/threonine-protein kinase PBL19 OS=Arabidopsis thaliana OX=3702 GN=PBL19 PE=1 SV=1)

HSP 1 Score: 418.3 bits (1074), Expect = 1.0e-115
Identity = 224/408 (54.90%), Postives = 289/408 (70.83%), Query Frame = 0

Query: 1   MKCFFYFKNKSRSRRH--------------QRSAPELKGELESDFSGCSQTAASSCSLTS 60
           M C F FK+K   ++               Q SAPEL    E+     S    +  SL S
Sbjct: 1   MNCLFLFKSKKPRKQQKDNNKNKRKGKELLQNSAPELTNRSETS----SFNLQTPRSLPS 60

Query: 61  TRSVPELYEEKAHNLRVFSFAEMREATHDFNRLLKIGQGGFGSVFKGSI-KPIDGKGDPL 120
            RS+ +LY E+  NLRVFS+ E+ +AT+ F+R L IG+GGFG V+KG I    D    PL
Sbjct: 61  PRSIKDLYTEREQNLRVFSYEELSKATYVFSRKLVIGEGGFGIVYKGKILSNGDSSDPPL 120

Query: 121 VVAIKQLSKDGLQGHKQWLAEVQFLGIVEHPNLVQLVGYCAVDGTRGIQRLLVYEYMTNR 180
           VVAIK+L++ GLQGHKQWLAEVQFLG+V HPN+V+L+GYC+ DG  GI+RLLVYEYM+NR
Sbjct: 121 VVAIKKLNRQGLQGHKQWLAEVQFLGVVNHPNVVKLIGYCSEDGETGIERLLVYEYMSNR 180

Query: 181 SLEDHLFNKALPTLAWKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPK 240
           SLEDHLF +   TL WK RL+I+LGAA+GL YLH   ++++IYRDFKSSNVLLD+ F PK
Sbjct: 181 SLEDHLFPRRSHTLPWKKRLEIMLGAAEGLTYLH---DLKVIYRDFKSSNVLLDDQFCPK 240

Query: 241 LSDFGLAREGPEFGRTHVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSYGVVLYEILTGRR 300
           LSDFGLAREGP+   THV+TA +GT+GYAAP+Y++TGHL  KSDV+S+GVVLYEI+TGRR
Sbjct: 241 LSDFGLAREGPDGDNTHVTTARVGTHGYAAPEYVQTGHLRLKSDVYSFGVVLYEIITGRR 300

Query: 301 SLERHHPRPEQNLVEWVKYINPDSEKFSSIIDPRLENEYPINTARKVAKLANTCLEKNAK 360
           ++ER+ P  E+ L++WVK    DS++FS I+DPRL N YP   AR +AKLA+ CL+KN K
Sbjct: 301 TIERNKPVAERRLLDWVKEYPADSQRFSMIVDPRLRNNYPAAGARSLAKLADLCLKKNDK 360

Query: 361 DRPSMAEVVNRLKQIIKAEDDNEPYEKSPDNSDNKPDMER----EPEK 390
           +RP+M  VV RLK+II+ E D+E Y  +   +     + R    +PEK
Sbjct: 361 ERPTMEIVVERLKKIIE-ESDSEDYPMATTTTKESSQVRRRQVAKPEK 400

BLAST of Carg06325 vs. ExPASy Swiss-Prot
Match: F4JPX3 (Probable serine/threonine-protein kinase PBL20 OS=Arabidopsis thaliana OX=3702 GN=PBL20 PE=3 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 1.2e-103
Identity = 211/390 (54.10%), Postives = 266/390 (68.21%), Query Frame = 0

Query: 1   MKCFFYF---KNKSRSRRH------------QRSAPELKGELESDFSGCSQTAASSCSLT 60
           M C F F   K KSR                Q+SAPEL     +     S    +  SL 
Sbjct: 1   MNCLFLFMSKKPKSRGNMEKEKKNIRGREFLQKSAPELTTRKTT----LSFNLPTPRSLP 60

Query: 61  STRSVPELYEEKA----HNLRVFSFAEMREATHDFNRLLKIGQGGFGSVFKGSI-KPI-- 120
           S  S+ +LY ++      NLRVFSF E+ +AT +F+R LKIG+GGFGSV+K +I  P   
Sbjct: 61  SPTSIKDLYTDREQNQNQNLRVFSFKELSDATCEFSRKLKIGEGGFGSVYKATINNPTVG 120

Query: 121 DGKGDPLVVAIKQLSKDGLQGHKQWLAEVQFLGIVEHPNLVQLVGYCAVDGTRGIQRLLV 180
           D    PL VA+K+L++  LQGHKQWLAEV FLG+V HPN+V+L+GYC+ D     +RLLV
Sbjct: 121 DSHSSPLTVAVKKLNRQSLQGHKQWLAEVHFLGVVNHPNVVRLLGYCSEDR----ERLLV 180

Query: 181 YEYMTNRSLEDHLFNKALPTLAWKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLL 240
           YE M+NRSLEDHLF     TL+WK RL+I+LGAAQGLAYLH   E+Q+IYRDFKSSNVLL
Sbjct: 181 YELMSNRSLEDHLFTLRTLTLSWKQRLEIMLGAAQGLAYLH---EIQVIYRDFKSSNVLL 240

Query: 241 DENFHPKLSDFGLAREGPEFGRTHVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSYGVVLY 300
           +E FHPKLSDFGLAREGPE   THV+TA +GT+GYAAP+Y+ TGHL    DV+S+GVVLY
Sbjct: 241 NEEFHPKLSDFGLAREGPEGDNTHVTTARVGTDGYAAPEYVITGHLKTHCDVYSFGVVLY 300

Query: 301 EILTGRRSLERHHPRPEQNLVEWVKYINPDSEKFSSIIDPRLENEYPINTARKVAKLANT 360
           EI+TGRR+LER  P  EQ L+EWVK    +S++F  I+D +L N+YPI   R+VAKLA+ 
Sbjct: 301 EIITGRRTLERMKPLAEQKLLEWVKKYPINSKRFKMIVDSKLCNKYPIAMVRRVAKLADH 360

Query: 361 CLEKNAKDRPSMAEVVNRLKQIIKAEDDNE 369
           C+ K  K+RP+MA VV  L  II+  +  +
Sbjct: 361 CVNKIDKERPTMAFVVESLTNIIEESNSED 379

BLAST of Carg06325 vs. ExPASy Swiss-Prot
Match: Q9SF86 (Serine/threonine-protein kinase PCRK1 OS=Arabidopsis thaliana OX=3702 GN=PCRK1 PE=1 SV=1)

HSP 1 Score: 374.8 bits (961), Expect = 1.3e-102
Identity = 192/385 (49.87%), Postives = 259/385 (67.27%), Query Frame = 0

Query: 1   MKCFFYFKNKSRSRRHQRSAPELKGELE--------SDFSGCSQTAASSCSLTSTRSVPE 60
           MKCF +     R  +    +  L             S+F+    +  S+ S    ++   
Sbjct: 1   MKCFLFSGGDKRGEQKTPISVSLTSIFSDREINRSGSEFNSRDVSGTSTESSMGRKNSYP 60

Query: 61  LYEEKAHNLRVFSFAEMREATHDFNRLLKIGQGGFGSVFKGSIKPIDGKGDPLVVAIKQL 120
               +A NLR FS  +++ AT +F+R + IG+GGFG VF+G+++ ++     + VA+KQL
Sbjct: 61  PVSTRASNLREFSITDLKSATKNFSRSVMIGEGGFGCVFRGTVRNLEDSSVKIEVAVKQL 120

Query: 121 SKDGLQGHKQWLAEVQFLGIVEHPNLVQLVGYCAVDGTRGIQRLLVYEYMTNRSLEDHLF 180
            K GLQGHK+W+ EV FLGIVEH NLV+L+GYCA D  RGIQRLLVYEYM NRS+E HL 
Sbjct: 121 GKRGLQGHKEWVTEVNFLGIVEHTNLVKLLGYCAEDDERGIQRLLVYEYMPNRSVEFHLS 180

Query: 181 NKALPTLAWKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLA 240
            ++L  L W  RL+I   AA+GL YLHE +E QII+RDFKSSN+LLDE++  KLSDFGLA
Sbjct: 181 PRSLTVLTWDLRLRIAQDAARGLTYLHEEMEFQIIFRDFKSSNILLDEDWKAKLSDFGLA 240

Query: 241 REGPEFGRTHVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSYGVVLYEILTGRRSLERHHP 300
           R GP  G THVST V+GT GYAAP+YI+TG LT+KSDVW YGV LYE++TGRR ++R+ P
Sbjct: 241 RLGPSEGLTHVSTDVVGTMGYAAPEYIQTGRLTSKSDVWGYGVFLYELITGRRPVDRNRP 300

Query: 301 RPEQNLVEWVKYINPDSEKFSSIIDPRLENEYPINTARKVAKLANTCLEKNAKDRPSMAE 360
           + EQ L+EWV+    D+ KF  I+DPRLE +YPI + +K+A +AN CL +N+K RP M+E
Sbjct: 301 KGEQKLLEWVRPYLSDTRKFKLILDPRLEGKYPIKSVQKLAVVANRCLVRNSKARPKMSE 360

Query: 361 VVNRLKQIIKAEDDNEPYEKSPDNS 378
           V+  + +I++A   N   +  P NS
Sbjct: 361 VLEMVNKIVEASSGNGSPQLVPLNS 385

BLAST of Carg06325 vs. ExPASy Swiss-Prot
Match: Q9LZF8 (Serine/threonine-protein kinase PCRK2 OS=Arabidopsis thaliana OX=3702 GN=PCRK2 PE=1 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 1.0e-99
Identity = 192/389 (49.36%), Postives = 260/389 (66.84%), Query Frame = 0

Query: 1   MKCFFY----FKNKSRSRRHQRSAPELK--GELESDFSGCSQTAASSCSLTSTRSVPELY 60
           MKCF +     K++ RS +            +  SDFS    +  S+ S T   S   + 
Sbjct: 1   MKCFLFPLGDKKDEQRSPKPVSPTSNFSDVNKSGSDFSPRDVSGTSTVSSTGRNSNTSM- 60

Query: 61  EEKAHNLRVFSFAEMREATHDFNRLLKIGQGGFGSVFKGSIKPIDGKGDPLVVAIKQLSK 120
             + +NLR F+  +++ AT +F+R   IG+GGFG VF G+IK ++     + VA+KQL K
Sbjct: 61  SARENNLREFTIGDLKSATRNFSRSGMIGEGGFGCVFWGTIKNLEDPSKKIEVAVKQLGK 120

Query: 121 DGLQGHKQWLAEVQFLGIVEHPNLVQLVGYCAVDGTRGIQRLLVYEYMTNRSLEDHLFNK 180
            GLQGHK+W+ EV FLG+VEH NLV+L+G+CA D  RGIQRLLVYEYM N+S+E HL  +
Sbjct: 121 RGLQGHKEWVTEVNFLGVVEHSNLVKLLGHCAEDDERGIQRLLVYEYMPNQSVEFHLSPR 180

Query: 181 ALPTLAWKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLARE 240
           +   L W  RL+I   AA+GL YLHE ++ QII+RDFKSSN+LLDEN+  KLSDFGLAR 
Sbjct: 181 SPTVLTWDLRLRIAQDAARGLTYLHEEMDFQIIFRDFKSSNILLDENWTAKLSDFGLARL 240

Query: 241 GPEFGRTHVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSYGVVLYEILTGRRSLERHHPRP 300
           GP  G +HVST V+GT GYAAP+YI+TG LT+KSDVW YGV +YE++TGRR L+R+ P+ 
Sbjct: 241 GPSPGSSHVSTDVVGTMGYAAPEYIQTGRLTSKSDVWGYGVFIYELITGRRPLDRNKPKG 300

Query: 301 EQNLVEWVKYINPDSEKFSSIIDPRLENEYPINTARKVAKLANTCLEKNAKDRPSMAEVV 360
           EQ L+EWV+    D+ +F  I+DPRLE +Y I + +K+A +AN CL +NAK RP M+EV+
Sbjct: 301 EQKLLEWVRPYLSDTRRFRLIVDPRLEGKYMIKSVQKLAVVANLCLTRNAKARPKMSEVL 360

Query: 361 NRLKQIIKAEDDNEPYEKSPDNSDNKPDM 384
             + +I++A         SP N   KP +
Sbjct: 361 EMVTKIVEA--------SSPGNGGKKPQL 380

BLAST of Carg06325 vs. ExPASy Swiss-Prot
Match: F4J0D2 (Serine/threonine-protein kinase PBL36 OS=Arabidopsis thaliana OX=3702 GN=PBL36 PE=1 SV=2)

HSP 1 Score: 330.1 bits (845), Expect = 3.7e-89
Identity = 174/339 (51.33%), Postives = 234/339 (69.03%), Query Frame = 0

Query: 32  SGCSQTAASSCSLTSTRSVPELYEEKAHNLRVFSFAEMREATHDFNRLLKIGQGGFGSVF 91
           +GC+++ +S    T   S    Y  K   LR+F F +++ AT +F     +G+GGFG VF
Sbjct: 89  TGCAESGSS----TPLMSGELKYSSK---LRIFMFNDLKLATRNFRPESLLGEGGFGCVF 148

Query: 92  KGSIK-----PIDGKGDPLVVAIKQLSKDGLQGHKQWLAEVQFLGIVEHPNLVQLVGYCA 151
           KG I+     P+   G  L VA+K L+ DGLQGHK+WLAE+ FLG + HP+LV+LVGYC 
Sbjct: 149 KGWIEENGTAPVK-PGTGLTVAVKTLNPDGLQGHKEWLAEINFLGNLVHPSLVKLVGYCM 208

Query: 152 VDGTRGIQRLLVYEYMTNRSLEDHLFNKALPTLAWKTRLQIVLGAAQGLAYLHEGLEVQI 211
            +     QRLLVYE+M   SLE+HLF + LP L W  R++I LGAA+GLA+LHE  E  +
Sbjct: 209 EED----QRLLVYEFMPRGSLENHLFRRTLP-LPWSVRMKIALGAAKGLAFLHEEAEKPV 268

Query: 212 IYRDFKSSNVLLDENFHPKLSDFGLAREGPEFGRTHVSTAVMGTNGYAAPDYIKTGHLTA 271
           IYRDFK+SN+LLD  ++ KLSDFGLA++ P+  ++HVST VMGT GYAAP+Y+ TGHLT 
Sbjct: 269 IYRDFKTSNILLDGEYNAKLSDFGLAKDAPDEKKSHVSTRVMGTYGYAAPEYVMTGHLTT 328

Query: 272 KSDVWSYGVVLYEILTGRRSLERHHPRPEQNLVEWVKYINPDSEKFSSIIDPRLENEYPI 331
           KSDV+S+GVVL EILTGRRS+++  P  EQNLVEWV+    D ++F  ++DPRLE  Y I
Sbjct: 329 KSDVYSFGVVLLEILTGRRSVDKSRPNGEQNLVEWVRPHLLDKKRFYRLLDPRLEGHYSI 388

Query: 332 NTARKVAKLANTCLEKNAKDRPSMAEVVNRLKQIIKAED 366
             A+K  ++A  CL +++K RP M+EVV  LK +   +D
Sbjct: 389 KGAQKATQVAAQCLNRDSKARPKMSEVVEALKPLPNLKD 414

BLAST of Carg06325 vs. ExPASy TrEMBL
Match: A0A6J1FKY9 (probable serine/threonine-protein kinase PBL19 OS=Cucurbita moschata OX=3662 GN=LOC111446363 PE=3 SV=1)

HSP 1 Score: 853.6 bits (2204), Expect = 3.6e-244
Identity = 425/427 (99.53%), Postives = 427/427 (100.00%), Query Frame = 0

Query: 1   MKCFFYFKNKSRSRRHQRSAPELKGELESDFSGCSQTAASSCSLTSTRSVPELYEEKAHN 60
           MKCFFYFKNKSRSRRHQRSAPELKGELES+FSGCSQTAASSCSLTSTRSVPELYEEKAHN
Sbjct: 1   MKCFFYFKNKSRSRRHQRSAPELKGELESNFSGCSQTAASSCSLTSTRSVPELYEEKAHN 60

Query: 61  LRVFSFAEMREATHDFNRLLKIGQGGFGSVFKGSIKPIDGKGDPLVVAIKQLSKDGLQGH 120
           LRVFSFAEMREATHDFNRLLKIGQGGFGSVFKGSIKPIDGKGDPLVVAIKQLSKDGLQGH
Sbjct: 61  LRVFSFAEMREATHDFNRLLKIGQGGFGSVFKGSIKPIDGKGDPLVVAIKQLSKDGLQGH 120

Query: 121 KQWLAEVQFLGIVEHPNLVQLVGYCAVDGTRGIQRLLVYEYMTNRSLEDHLFNKALPTLA 180
           KQWLAEVQFLGIVEHPNLVQLVGYCAVDGTRGIQRLLVYEYMTNRSLEDHLFNKALPTLA
Sbjct: 121 KQWLAEVQFLGIVEHPNLVQLVGYCAVDGTRGIQRLLVYEYMTNRSLEDHLFNKALPTLA 180

Query: 181 WKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEFGR 240
           WKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEFGR
Sbjct: 181 WKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEFGR 240

Query: 241 THVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSYGVVLYEILTGRRSLERHHPRPEQNLVE 300
           THVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSYGVVLYEILTGRRSLERHHPRPEQNLVE
Sbjct: 241 THVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSYGVVLYEILTGRRSLERHHPRPEQNLVE 300

Query: 301 WVKYINPDSEKFSSIIDPRLENEYPINTARKVAKLANTCLEKNAKDRPSMAEVVNRLKQI 360
           WVKYINPDSEKFSSIIDPRLENEYPINTARKVAKLANTCLEKNAKDRPSMAEVVNRLKQI
Sbjct: 301 WVKYINPDSEKFSSIIDPRLENEYPINTARKVAKLANTCLEKNAKDRPSMAEVVNRLKQI 360

Query: 361 IKAEDDNEPYEKSPDNSDNKPDMEREPEKTKVMDSWRRRMSHLQKLGEHVEGASRRRFMI 420
           IKA+DDNEPYEKSPDNSDNKPDMEREPEKTKVMDSWRRRMSHLQKLGEHVEGASRRRFMI
Sbjct: 361 IKAKDDNEPYEKSPDNSDNKPDMEREPEKTKVMDSWRRRMSHLQKLGEHVEGASRRRFMI 420

Query: 421 MQRAKVP 428
           MQRAKVP
Sbjct: 421 MQRAKVP 427

BLAST of Carg06325 vs. ExPASy TrEMBL
Match: A0A6J1IB57 (probable serine/threonine-protein kinase PBL19 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111473487 PE=3 SV=1)

HSP 1 Score: 849.0 bits (2192), Expect = 8.8e-243
Identity = 421/427 (98.59%), Postives = 427/427 (100.00%), Query Frame = 0

Query: 1   MKCFFYFKNKSRSRRHQRSAPELKGELESDFSGCSQTAASSCSLTSTRSVPELYEEKAHN 60
           MKCFFYF+NKSRSRRHQRSAPELKGELESDFSGCS+TAASSCSLTSTRSVPELYEEKAHN
Sbjct: 1   MKCFFYFRNKSRSRRHQRSAPELKGELESDFSGCSRTAASSCSLTSTRSVPELYEEKAHN 60

Query: 61  LRVFSFAEMREATHDFNRLLKIGQGGFGSVFKGSIKPIDGKGDPLVVAIKQLSKDGLQGH 120
           LRVFSFAEMREATHDFNRLLKIGQGGFGSVFKGSIKPIDGKGDPLVVAIKQLSKDGLQGH
Sbjct: 61  LRVFSFAEMREATHDFNRLLKIGQGGFGSVFKGSIKPIDGKGDPLVVAIKQLSKDGLQGH 120

Query: 121 KQWLAEVQFLGIVEHPNLVQLVGYCAVDGTRGIQRLLVYEYMTNRSLEDHLFNKALPTLA 180
           KQWLAEVQFLGIVEHPNLVQLVGYCAVDGTRGIQRLL+YEY+TNRSLEDHLFNKALPTLA
Sbjct: 121 KQWLAEVQFLGIVEHPNLVQLVGYCAVDGTRGIQRLLIYEYVTNRSLEDHLFNKALPTLA 180

Query: 181 WKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEFGR 240
           WKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEFGR
Sbjct: 181 WKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEFGR 240

Query: 241 THVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSYGVVLYEILTGRRSLERHHPRPEQNLVE 300
           THVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSYGVVLYEILTGRRSLERHHPRPEQNLVE
Sbjct: 241 THVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSYGVVLYEILTGRRSLERHHPRPEQNLVE 300

Query: 301 WVKYINPDSEKFSSIIDPRLENEYPINTARKVAKLANTCLEKNAKDRPSMAEVVNRLKQI 360
           WVKYINPDS+KFSSIIDPRLENEYPINTARKVAKLANTCLEKNAKDRPSMAEVVNRLKQI
Sbjct: 301 WVKYINPDSKKFSSIIDPRLENEYPINTARKVAKLANTCLEKNAKDRPSMAEVVNRLKQI 360

Query: 361 IKAEDDNEPYEKSPDNSDNKPDMEREPEKTKVMDSWRRRMSHLQKLGEHVEGASRRRFMI 420
           IKAEDD+EPYEKSPDNSDNKPDMEREPEKTKVMDSWRRRMSHLQKLGEHVEGASRRRFMI
Sbjct: 361 IKAEDDDEPYEKSPDNSDNKPDMEREPEKTKVMDSWRRRMSHLQKLGEHVEGASRRRFMI 420

Query: 421 MQRAKVP 428
           MQRAKVP
Sbjct: 421 MQRAKVP 427

BLAST of Carg06325 vs. ExPASy TrEMBL
Match: A0A6J1IC97 (probable serine/threonine-protein kinase PBL19 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111473487 PE=3 SV=1)

HSP 1 Score: 834.3 bits (2154), Expect = 2.2e-238
Identity = 421/454 (92.73%), Postives = 427/454 (94.05%), Query Frame = 0

Query: 1   MKCFFYFKNKSRSRRHQRSAPELKGELESDFSGCSQTAASSCSLTSTRSVPELYEEKAHN 60
           MKCFFYF+NKSRSRRHQRSAPELKGELESDFSGCS+TAASSCSLTSTRSVPELYEEKAHN
Sbjct: 1   MKCFFYFRNKSRSRRHQRSAPELKGELESDFSGCSRTAASSCSLTSTRSVPELYEEKAHN 60

Query: 61  LRVFSFAEMREATHDFNRLLKIGQGGFGSVFKGSIKPIDGKGDPLVVAIKQLSKDGLQGH 120
           LRVFSFAEMREATHDFNRLLKIGQGGFGSVFKGSIKPIDGKGDPLVVAIKQLSKDGLQGH
Sbjct: 61  LRVFSFAEMREATHDFNRLLKIGQGGFGSVFKGSIKPIDGKGDPLVVAIKQLSKDGLQGH 120

Query: 121 KQWLAEVQFLGIVEHPNLVQLVGYCAVDGTRGIQRLLVYEYMTNRSLEDHLFNKALPTLA 180
           KQWLAEVQFLGIVEHPNLVQLVGYCAVDGTRGIQRLL+YEY+TNRSLEDHLFNKALPTLA
Sbjct: 121 KQWLAEVQFLGIVEHPNLVQLVGYCAVDGTRGIQRLLIYEYVTNRSLEDHLFNKALPTLA 180

Query: 181 WKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEFGR 240
           WKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEFGR
Sbjct: 181 WKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEFGR 240

Query: 241 THVSTA---------------------------VMGTNGYAAPDYIKTGHLTAKSDVWSY 300
           THVSTA                           VMGTNGYAAPDYIKTGHLTAKSDVWSY
Sbjct: 241 THVSTAVSVCLFFYSSPFNQLQMLTVNYIMYTQVMGTNGYAAPDYIKTGHLTAKSDVWSY 300

Query: 301 GVVLYEILTGRRSLERHHPRPEQNLVEWVKYINPDSEKFSSIIDPRLENEYPINTARKVA 360
           GVVLYEILTGRRSLERHHPRPEQNLVEWVKYINPDS+KFSSIIDPRLENEYPINTARKVA
Sbjct: 301 GVVLYEILTGRRSLERHHPRPEQNLVEWVKYINPDSKKFSSIIDPRLENEYPINTARKVA 360

Query: 361 KLANTCLEKNAKDRPSMAEVVNRLKQIIKAEDDNEPYEKSPDNSDNKPDMEREPEKTKVM 420
           KLANTCLEKNAKDRPSMAEVVNRLKQIIKAEDD+EPYEKSPDNSDNKPDMEREPEKTKVM
Sbjct: 361 KLANTCLEKNAKDRPSMAEVVNRLKQIIKAEDDDEPYEKSPDNSDNKPDMEREPEKTKVM 420

Query: 421 DSWRRRMSHLQKLGEHVEGASRRRFMIMQRAKVP 428
           DSWRRRMSHLQKLGEHVEGASRRRFMIMQRAKVP
Sbjct: 421 DSWRRRMSHLQKLGEHVEGASRRRFMIMQRAKVP 454

BLAST of Carg06325 vs. ExPASy TrEMBL
Match: A0A6J1EFA4 (probable serine/threonine-protein kinase PBL19 OS=Cucurbita moschata OX=3662 GN=LOC111431988 PE=3 SV=1)

HSP 1 Score: 752.3 bits (1941), Expect = 1.1e-213
Identity = 376/427 (88.06%), Postives = 397/427 (92.97%), Query Frame = 0

Query: 1   MKCFFYFKNKSRSRRHQRSAPELKGELESDFSGCSQTAASSCSLTSTRSVPELYEEKAHN 60
           MKCFFYF+NK+RS RHQRSAPELK + ESD SGCS+TAAS  SLTS RSVPELYEEKAHN
Sbjct: 1   MKCFFYFRNKNRS-RHQRSAPELKEQQESDISGCSRTAASLSSLTSPRSVPELYEEKAHN 60

Query: 61  LRVFSFAEMREATHDFNRLLKIGQGGFGSVFKGSIKPIDGKGDPLVVAIKQLSKDGLQGH 120
           LRVFSF E+R+ATHDFNRLLKIGQGGFGSVFKGSIKP+DGKG+PLVVAIKQLSKDGLQGH
Sbjct: 61  LRVFSFTELRQATHDFNRLLKIGQGGFGSVFKGSIKPVDGKGEPLVVAIKQLSKDGLQGH 120

Query: 121 KQWLAEVQFLGIVEHPNLVQLVGYCAVDGTRGIQRLLVYEYMTNRSLEDHLFNKALPTLA 180
           KQWLAEVQFL IVEHPNLV+L+GYCAVDG RGIQRLLVYEYM NRSLEDHLF KALP LA
Sbjct: 121 KQWLAEVQFLSIVEHPNLVKLIGYCAVDGERGIQRLLVYEYMPNRSLEDHLFKKALPALA 180

Query: 181 WKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEFGR 240
           WKTRLQIVLGAAQGLAYLHEGLE+QIIYRDFKSSNVLLDENFHPKLSDFGLAREGPE GR
Sbjct: 181 WKTRLQIVLGAAQGLAYLHEGLEIQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEIGR 240

Query: 241 THVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSYGVVLYEILTGRRSLERHHPRPEQNLVE 300
           THVSTAVMGTNGYAAPDYIKTGHLTAKSDVWS GVVLYEILTGRRSLERH  RPEQ LVE
Sbjct: 241 THVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSLGVVLYEILTGRRSLERHRSRPEQKLVE 300

Query: 301 WVKYINPDSEKFSSIIDPRLENEYPINTARKVAKLANTCLEKNAKDRPSMAEVVNRLKQI 360
           WVK+ NPDS +FS IIDPRLEN+YPIN ARKVAKLA+TCL KNAKDRPSMAEVVNRLK I
Sbjct: 301 WVKHFNPDSNRFSLIIDPRLENQYPINAARKVAKLADTCLAKNAKDRPSMAEVVNRLKLI 360

Query: 361 IKAEDDNEPYEKSPDNSDNKPDMEREPEKTKVMDSWRRRMSHLQKLGEHVEGASRRRFMI 420
           IKAEDD+EPY KSPD+ +++PDMEREPEK +   SWRRRMSHLQKLGEHVEGASRRRFMI
Sbjct: 361 IKAEDDSEPYAKSPDSVNDEPDMEREPEKMEAPMSWRRRMSHLQKLGEHVEGASRRRFMI 420

Query: 421 MQRAKVP 428
           MQRAKVP
Sbjct: 421 MQRAKVP 426

BLAST of Carg06325 vs. ExPASy TrEMBL
Match: A0A6J1IYW5 (probable serine/threonine-protein kinase PBL19 OS=Cucurbita maxima OX=3661 GN=LOC111479715 PE=3 SV=1)

HSP 1 Score: 745.3 bits (1923), Expect = 1.4e-211
Identity = 374/427 (87.59%), Postives = 395/427 (92.51%), Query Frame = 0

Query: 1   MKCFFYFKNKSRSRRHQRSAPELKGELESDFSGCSQTAASSCSLTSTRSVPELYEEKAHN 60
           MKCFFYF+NK+RS RHQRSAPELK + ESD SGCS+TAAS  SLTS RSVPELYEEKAHN
Sbjct: 1   MKCFFYFRNKNRS-RHQRSAPELKEQQESDISGCSRTAASLSSLTSPRSVPELYEEKAHN 60

Query: 61  LRVFSFAEMREATHDFNRLLKIGQGGFGSVFKGSIKPIDGKGDPLVVAIKQLSKDGLQGH 120
           LRVFSF E+R+ATHDFNRLLKIGQGGFGSVFKGSIKP+DGKG+PLVVAIKQLSKDGLQGH
Sbjct: 61  LRVFSFTELRQATHDFNRLLKIGQGGFGSVFKGSIKPVDGKGEPLVVAIKQLSKDGLQGH 120

Query: 121 KQWLAEVQFLGIVEHPNLVQLVGYCAVDGTRGIQRLLVYEYMTNRSLEDHLFNKALPTLA 180
           KQWLAEVQFL IVEHPNLV+L+GYCAVDG RGIQRLLVYEYM NRSLEDHLFNKALP LA
Sbjct: 121 KQWLAEVQFLSIVEHPNLVKLIGYCAVDGERGIQRLLVYEYMPNRSLEDHLFNKALPALA 180

Query: 181 WKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEFGR 240
           WKTRLQIVLGAAQGLAYLHEGLE+QIIYRDFKSSNVLLDENFHPKLSDFGLAREGPE G 
Sbjct: 181 WKTRLQIVLGAAQGLAYLHEGLEIQIIYRDFKSSNVLLDENFHPKLSDFGLAREGPEIGH 240

Query: 241 THVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSYGVVLYEILTGRRSLERHHPRPEQNLVE 300
           THVSTAVMGTNGYAAPDYIKTGHLTAKSDVWS GVVLYEILTGRRSLERH  R EQ LVE
Sbjct: 241 THVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSLGVVLYEILTGRRSLERHRSRLEQRLVE 300

Query: 301 WVKYINPDSEKFSSIIDPRLENEYPINTARKVAKLANTCLEKNAKDRPSMAEVVNRLKQI 360
           WVK+ NPDS +FS IIDPRLEN+YPIN ARKVAKLA+TCL KNAKDRPSMAEVVNRLK I
Sbjct: 301 WVKHFNPDSNRFSLIIDPRLENQYPINAARKVAKLADTCLAKNAKDRPSMAEVVNRLKLI 360

Query: 361 IKAEDDNEPYEKSPDNSDNKPDMEREPEKTKVMDSWRRRMSHLQKLGEHVEGASRRRFMI 420
           IKAED +EPY KSPD+ +++PDMEREPEK +   SWRRRMSHLQKLGEHVEGASRRRFMI
Sbjct: 361 IKAEDVSEPYAKSPDSVNDEPDMEREPEKMEAPMSWRRRMSHLQKLGEHVEGASRRRFMI 420

Query: 421 MQRAKVP 428
           MQRAKVP
Sbjct: 421 MQRAKVP 426

BLAST of Carg06325 vs. TAIR 10
Match: AT5G47070.1 (Protein kinase superfamily protein )

HSP 1 Score: 418.3 bits (1074), Expect = 7.4e-117
Identity = 224/408 (54.90%), Postives = 289/408 (70.83%), Query Frame = 0

Query: 1   MKCFFYFKNKSRSRRH--------------QRSAPELKGELESDFSGCSQTAASSCSLTS 60
           M C F FK+K   ++               Q SAPEL    E+     S    +  SL S
Sbjct: 1   MNCLFLFKSKKPRKQQKDNNKNKRKGKELLQNSAPELTNRSETS----SFNLQTPRSLPS 60

Query: 61  TRSVPELYEEKAHNLRVFSFAEMREATHDFNRLLKIGQGGFGSVFKGSI-KPIDGKGDPL 120
            RS+ +LY E+  NLRVFS+ E+ +AT+ F+R L IG+GGFG V+KG I    D    PL
Sbjct: 61  PRSIKDLYTEREQNLRVFSYEELSKATYVFSRKLVIGEGGFGIVYKGKILSNGDSSDPPL 120

Query: 121 VVAIKQLSKDGLQGHKQWLAEVQFLGIVEHPNLVQLVGYCAVDGTRGIQRLLVYEYMTNR 180
           VVAIK+L++ GLQGHKQWLAEVQFLG+V HPN+V+L+GYC+ DG  GI+RLLVYEYM+NR
Sbjct: 121 VVAIKKLNRQGLQGHKQWLAEVQFLGVVNHPNVVKLIGYCSEDGETGIERLLVYEYMSNR 180

Query: 181 SLEDHLFNKALPTLAWKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPK 240
           SLEDHLF +   TL WK RL+I+LGAA+GL YLH   ++++IYRDFKSSNVLLD+ F PK
Sbjct: 181 SLEDHLFPRRSHTLPWKKRLEIMLGAAEGLTYLH---DLKVIYRDFKSSNVLLDDQFCPK 240

Query: 241 LSDFGLAREGPEFGRTHVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSYGVVLYEILTGRR 300
           LSDFGLAREGP+   THV+TA +GT+GYAAP+Y++TGHL  KSDV+S+GVVLYEI+TGRR
Sbjct: 241 LSDFGLAREGPDGDNTHVTTARVGTHGYAAPEYVQTGHLRLKSDVYSFGVVLYEIITGRR 300

Query: 301 SLERHHPRPEQNLVEWVKYINPDSEKFSSIIDPRLENEYPINTARKVAKLANTCLEKNAK 360
           ++ER+ P  E+ L++WVK    DS++FS I+DPRL N YP   AR +AKLA+ CL+KN K
Sbjct: 301 TIERNKPVAERRLLDWVKEYPADSQRFSMIVDPRLRNNYPAAGARSLAKLADLCLKKNDK 360

Query: 361 DRPSMAEVVNRLKQIIKAEDDNEPYEKSPDNSDNKPDMER----EPEK 390
           +RP+M  VV RLK+II+ E D+E Y  +   +     + R    +PEK
Sbjct: 361 ERPTMEIVVERLKKIIE-ESDSEDYPMATTTTKESSQVRRRQVAKPEK 400

BLAST of Carg06325 vs. TAIR 10
Match: AT2G39110.1 (Protein kinase superfamily protein )

HSP 1 Score: 394.4 bits (1012), Expect = 1.1e-109
Identity = 201/374 (53.74%), Postives = 268/374 (71.66%), Query Frame = 0

Query: 1   MKCFFYFKNKSR----SRRHQRSAPELKGELESDFSGCSQTAASSCSLTSTRSVPELYEE 60
           MKCF++ K+K++      R   SA   +G   S+F+  + TA S      T S+  L E 
Sbjct: 16  MKCFYFSKDKTQDDEAKTRKFGSATMARGGSGSEFNSDTSTATS-----ITSSLHVLSET 75

Query: 61  KAHNLRVFSFAEMREATHDFNRLLKIGQGGFGSVFKGSIKPIDGKGDPLVVAIKQLSKDG 120
            ++NL+VF   +++ AT +F+R L IG+GGFG VF+G I+        + +A+KQLS+ G
Sbjct: 76  HSNNLKVFVLDDLKTATKNFSRSLMIGEGGFGGVFRGVIQNPQDSRKKIDIAVKQLSRRG 135

Query: 121 LQGHKQWLAEVQFLGIVEHPNLVQLVGYCAVDGTRGIQRLLVYEYMTNRSLEDHLFNKAL 180
           LQGHK+W+ EV  LG+VEHPNLV+L+GYCA D  RGIQRLLVYEY+ NRS++DHL N+ +
Sbjct: 136 LQGHKEWVTEVNVLGVVEHPNLVKLIGYCAEDDERGIQRLLVYEYVQNRSVQDHLSNRFI 195

Query: 181 PT-LAWKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLAREG 240
            T L W TRL+I    A+GLAYLH+G+E QII+RDFKSSN+LLDEN++ KLSDFGLAR G
Sbjct: 196 VTPLPWSTRLKIAQDTARGLAYLHQGMEFQIIFRDFKSSNILLDENWNAKLSDFGLARMG 255

Query: 241 PEFGRTHVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSYGVVLYEILTGRRSLERHHPRPE 300
           P  G THVSTAV+GT GYAAP+YI+TGHLTAKSDVWSYG+ LYE++TGRR  +R+ PR E
Sbjct: 256 PSDGITHVSTAVVGTIGYAAPEYIQTGHLTAKSDVWSYGIFLYELITGRRPFDRNRPRNE 315

Query: 301 QNLVEWVKYINPDSEKFSSIIDPRLENEYPINTARKVAKLANTCLEKNAKDRPSMAEVVN 360
           QN++EW++    D +KF  IIDPRLE  Y + +A K+A +AN CL   AK RP+M++V  
Sbjct: 316 QNILEWIRPHLSDIKKFKMIIDPRLEGNYYLKSALKLAAVANRCLMVKAKARPTMSQVSE 375

Query: 361 RLKQIIKAEDDNEP 370
            L++I++   D  P
Sbjct: 376 MLERIVETSSDGAP 384

BLAST of Carg06325 vs. TAIR 10
Match: AT4G17660.1 (Protein kinase superfamily protein )

HSP 1 Score: 378.3 bits (970), Expect = 8.4e-105
Identity = 211/390 (54.10%), Postives = 266/390 (68.21%), Query Frame = 0

Query: 1   MKCFFYF---KNKSRSRRH------------QRSAPELKGELESDFSGCSQTAASSCSLT 60
           M C F F   K KSR                Q+SAPEL     +     S    +  SL 
Sbjct: 1   MNCLFLFMSKKPKSRGNMEKEKKNIRGREFLQKSAPELTTRKTT----LSFNLPTPRSLP 60

Query: 61  STRSVPELYEEKA----HNLRVFSFAEMREATHDFNRLLKIGQGGFGSVFKGSI-KPI-- 120
           S  S+ +LY ++      NLRVFSF E+ +AT +F+R LKIG+GGFGSV+K +I  P   
Sbjct: 61  SPTSIKDLYTDREQNQNQNLRVFSFKELSDATCEFSRKLKIGEGGFGSVYKATINNPTVG 120

Query: 121 DGKGDPLVVAIKQLSKDGLQGHKQWLAEVQFLGIVEHPNLVQLVGYCAVDGTRGIQRLLV 180
           D    PL VA+K+L++  LQGHKQWLAEV FLG+V HPN+V+L+GYC+ D     +RLLV
Sbjct: 121 DSHSSPLTVAVKKLNRQSLQGHKQWLAEVHFLGVVNHPNVVRLLGYCSEDR----ERLLV 180

Query: 181 YEYMTNRSLEDHLFNKALPTLAWKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLL 240
           YE M+NRSLEDHLF     TL+WK RL+I+LGAAQGLAYLH   E+Q+IYRDFKSSNVLL
Sbjct: 181 YELMSNRSLEDHLFTLRTLTLSWKQRLEIMLGAAQGLAYLH---EIQVIYRDFKSSNVLL 240

Query: 241 DENFHPKLSDFGLAREGPEFGRTHVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSYGVVLY 300
           +E FHPKLSDFGLAREGPE   THV+TA +GT+GYAAP+Y+ TGHL    DV+S+GVVLY
Sbjct: 241 NEEFHPKLSDFGLAREGPEGDNTHVTTARVGTDGYAAPEYVITGHLKTHCDVYSFGVVLY 300

Query: 301 EILTGRRSLERHHPRPEQNLVEWVKYINPDSEKFSSIIDPRLENEYPINTARKVAKLANT 360
           EI+TGRR+LER  P  EQ L+EWVK    +S++F  I+D +L N+YPI   R+VAKLA+ 
Sbjct: 301 EIITGRRTLERMKPLAEQKLLEWVKKYPINSKRFKMIVDSKLCNKYPIAMVRRVAKLADH 360

Query: 361 CLEKNAKDRPSMAEVVNRLKQIIKAEDDNE 369
           C+ K  K+RP+MA VV  L  II+  +  +
Sbjct: 361 CVNKIDKERPTMAFVVESLTNIIEESNSED 379

BLAST of Carg06325 vs. TAIR 10
Match: AT3G09830.1 (Protein kinase superfamily protein )

HSP 1 Score: 374.8 bits (961), Expect = 9.3e-104
Identity = 192/385 (49.87%), Postives = 259/385 (67.27%), Query Frame = 0

Query: 1   MKCFFYFKNKSRSRRHQRSAPELKGELE--------SDFSGCSQTAASSCSLTSTRSVPE 60
           MKCF +     R  +    +  L             S+F+    +  S+ S    ++   
Sbjct: 1   MKCFLFSGGDKRGEQKTPISVSLTSIFSDREINRSGSEFNSRDVSGTSTESSMGRKNSYP 60

Query: 61  LYEEKAHNLRVFSFAEMREATHDFNRLLKIGQGGFGSVFKGSIKPIDGKGDPLVVAIKQL 120
               +A NLR FS  +++ AT +F+R + IG+GGFG VF+G+++ ++     + VA+KQL
Sbjct: 61  PVSTRASNLREFSITDLKSATKNFSRSVMIGEGGFGCVFRGTVRNLEDSSVKIEVAVKQL 120

Query: 121 SKDGLQGHKQWLAEVQFLGIVEHPNLVQLVGYCAVDGTRGIQRLLVYEYMTNRSLEDHLF 180
            K GLQGHK+W+ EV FLGIVEH NLV+L+GYCA D  RGIQRLLVYEYM NRS+E HL 
Sbjct: 121 GKRGLQGHKEWVTEVNFLGIVEHTNLVKLLGYCAEDDERGIQRLLVYEYMPNRSVEFHLS 180

Query: 181 NKALPTLAWKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLA 240
            ++L  L W  RL+I   AA+GL YLHE +E QII+RDFKSSN+LLDE++  KLSDFGLA
Sbjct: 181 PRSLTVLTWDLRLRIAQDAARGLTYLHEEMEFQIIFRDFKSSNILLDEDWKAKLSDFGLA 240

Query: 241 REGPEFGRTHVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSYGVVLYEILTGRRSLERHHP 300
           R GP  G THVST V+GT GYAAP+YI+TG LT+KSDVW YGV LYE++TGRR ++R+ P
Sbjct: 241 RLGPSEGLTHVSTDVVGTMGYAAPEYIQTGRLTSKSDVWGYGVFLYELITGRRPVDRNRP 300

Query: 301 RPEQNLVEWVKYINPDSEKFSSIIDPRLENEYPINTARKVAKLANTCLEKNAKDRPSMAE 360
           + EQ L+EWV+    D+ KF  I+DPRLE +YPI + +K+A +AN CL +N+K RP M+E
Sbjct: 301 KGEQKLLEWVRPYLSDTRKFKLILDPRLEGKYPIKSVQKLAVVANRCLVRNSKARPKMSE 360

Query: 361 VVNRLKQIIKAEDDNEPYEKSPDNS 378
           V+  + +I++A   N   +  P NS
Sbjct: 361 VLEMVNKIVEASSGNGSPQLVPLNS 385

BLAST of Carg06325 vs. TAIR 10
Match: AT3G09830.2 (Protein kinase superfamily protein )

HSP 1 Score: 374.8 bits (961), Expect = 9.3e-104
Identity = 192/385 (49.87%), Postives = 259/385 (67.27%), Query Frame = 0

Query: 1   MKCFFYFKNKSRSRRHQRSAPELKGELE--------SDFSGCSQTAASSCSLTSTRSVPE 60
           MKCF +     R  +    +  L             S+F+    +  S+ S    ++   
Sbjct: 1   MKCFLFSGGDKRGEQKTPISVSLTSIFSDREINRSGSEFNSRDVSGTSTESSMGRKNSYP 60

Query: 61  LYEEKAHNLRVFSFAEMREATHDFNRLLKIGQGGFGSVFKGSIKPIDGKGDPLVVAIKQL 120
               +A NLR FS  +++ AT +F+R + IG+GGFG VF+G+++ ++     + VA+KQL
Sbjct: 61  PVSTRASNLREFSITDLKSATKNFSRSVMIGEGGFGCVFRGTVRNLEDSSVKIEVAVKQL 120

Query: 121 SKDGLQGHKQWLAEVQFLGIVEHPNLVQLVGYCAVDGTRGIQRLLVYEYMTNRSLEDHLF 180
            K GLQGHK+W+ EV FLGIVEH NLV+L+GYCA D  RGIQRLLVYEYM NRS+E HL 
Sbjct: 121 GKRGLQGHKEWVTEVNFLGIVEHTNLVKLLGYCAEDDERGIQRLLVYEYMPNRSVEFHLS 180

Query: 181 NKALPTLAWKTRLQIVLGAAQGLAYLHEGLEVQIIYRDFKSSNVLLDENFHPKLSDFGLA 240
            ++L  L W  RL+I   AA+GL YLHE +E QII+RDFKSSN+LLDE++  KLSDFGLA
Sbjct: 181 PRSLTVLTWDLRLRIAQDAARGLTYLHEEMEFQIIFRDFKSSNILLDEDWKAKLSDFGLA 240

Query: 241 REGPEFGRTHVSTAVMGTNGYAAPDYIKTGHLTAKSDVWSYGVVLYEILTGRRSLERHHP 300
           R GP  G THVST V+GT GYAAP+YI+TG LT+KSDVW YGV LYE++TGRR ++R+ P
Sbjct: 241 RLGPSEGLTHVSTDVVGTMGYAAPEYIQTGRLTSKSDVWGYGVFLYELITGRRPVDRNRP 300

Query: 301 RPEQNLVEWVKYINPDSEKFSSIIDPRLENEYPINTARKVAKLANTCLEKNAKDRPSMAE 360
           + EQ L+EWV+    D+ KF  I+DPRLE +YPI + +K+A +AN CL +N+K RP M+E
Sbjct: 301 KGEQKLLEWVRPYLSDTRKFKLILDPRLEGKYPIKSVQKLAVVANRCLVRNSKARPKMSE 360

Query: 361 VVNRLKQIIKAEDDNEPYEKSPDNS 378
           V+  + +I++A   N   +  P NS
Sbjct: 361 VLEMVNKIVEASSGNGSPQLVPLNS 385

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7028725.16.7e-245100.00putative serine/threonine-protein kinase PBL19 [Cucurbita argyrosperma subsp. ar... [more]
XP_022940922.17.4e-24499.53probable serine/threonine-protein kinase PBL19 [Cucurbita moschata][more]
XP_023540606.16.2e-24399.06probable serine/threonine-protein kinase PBL19 isoform X2 [Cucurbita pepo subsp.... [more]
XP_022974762.11.8e-24298.59probable serine/threonine-protein kinase PBL19 isoform X2 [Cucurbita maxima][more]
XP_023540605.11.6e-23893.17probable serine/threonine-protein kinase PBL19 isoform X1 [Cucurbita pepo subsp.... [more]
Match NameE-valueIdentityDescription
Q9LTC01.0e-11554.90Probable serine/threonine-protein kinase PBL19 OS=Arabidopsis thaliana OX=3702 G... [more]
F4JPX31.2e-10354.10Probable serine/threonine-protein kinase PBL20 OS=Arabidopsis thaliana OX=3702 G... [more]
Q9SF861.3e-10249.87Serine/threonine-protein kinase PCRK1 OS=Arabidopsis thaliana OX=3702 GN=PCRK1 P... [more]
Q9LZF81.0e-9949.36Serine/threonine-protein kinase PCRK2 OS=Arabidopsis thaliana OX=3702 GN=PCRK2 P... [more]
F4J0D23.7e-8951.33Serine/threonine-protein kinase PBL36 OS=Arabidopsis thaliana OX=3702 GN=PBL36 P... [more]
Match NameE-valueIdentityDescription
A0A6J1FKY93.6e-24499.53probable serine/threonine-protein kinase PBL19 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1IB578.8e-24398.59probable serine/threonine-protein kinase PBL19 isoform X2 OS=Cucurbita maxima OX... [more]
A0A6J1IC972.2e-23892.73probable serine/threonine-protein kinase PBL19 isoform X1 OS=Cucurbita maxima OX... [more]
A0A6J1EFA41.1e-21388.06probable serine/threonine-protein kinase PBL19 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1IYW51.4e-21187.59probable serine/threonine-protein kinase PBL19 OS=Cucurbita maxima OX=3661 GN=LO... [more]
Match NameE-valueIdentityDescription
AT5G47070.17.4e-11754.90Protein kinase superfamily protein [more]
AT2G39110.11.1e-10953.74Protein kinase superfamily protein [more]
AT4G17660.18.4e-10554.10Protein kinase superfamily protein [more]
AT3G09830.19.3e-10449.87Protein kinase superfamily protein [more]
AT3G09830.29.3e-10449.87Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 81..357
e-value: 1.0E-47
score: 162.6
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 162..383
e-value: 4.9E-64
score: 217.5
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 48..161
e-value: 2.3E-26
score: 93.9
NoneNo IPR availablePIRSRPIRSR037921-2PIRSR037921-2coord: 79..365
e-value: 1.0E-22
score: 78.7
NoneNo IPR availablePIRSRPIRSR633573-1PIRSR633573-1coord: 81..288
e-value: 6.9E-12
score: 43.0
NoneNo IPR availablePIRSRPIRSR000556-1PIRSR000556-1coord: 76..284
e-value: 5.8E-27
score: 92.3
NoneNo IPR availablePIRSRPIRSR038172-1PIRSR038172-1coord: 70..289
e-value: 8.8E-10
score: 35.4
NoneNo IPR availablePIRSRPIRSR000605-51PIRSR000605-51coord: 68..284
e-value: 1.4E-19
score: 68.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 362..395
NoneNo IPR availablePANTHERPTHR45621:SF190OS07G0495150 PROTEINcoord: 29..411
NoneNo IPR availablePANTHERPTHR45621OS01G0588500 PROTEIN-RELATEDcoord: 29..411
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 82..110
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 206..218
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 76..361
score: 39.820438
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 59..365

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg06325-RACarg06325-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity