Carg06181 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg06181
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionADP-ribosylation factor-like protein 5
LocationCarg_Chr06: 8640004 .. 8642401 (+)
RNA-Seq ExpressionCarg06181
SyntenyCarg06181
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATCAAATTGATCCTTCTGCCAGTGTCCATCCACACTTTCGCGCTACGAAGCTCCTTAACCTTGCCGACCAGAAATATATCCCGCTGCCCCCCCAAAACTTCGGACAACTTATTACCTCACCTGAATTCTCGACGGATTTAGTGGTTTTCTCTATCTGTGTGTCCCTTGATTCCGGTTTTTGTAAAAAATGCTATTCTGGTCCACTTTCGCCGCTCGATTAATTAAAGGTATTGCAGATGCATTGCAGCGCACTCCTATTCATGTCTTTTATAAAGCGCCTTTTTCGTTTTCTGATCAAACATCTCCTGCGTCTCGTTAGTGGATTGTGAGTTTGTTTCCGTCTGTCCGATTGTGCATTTAGTTAGGTTTTCTTTCTAGGAGTTATTTATTACTTCTGGGATTTAGCTATGAATTTCATGTTGCTGTTTGATGAAGAAATTTAATGTGTGTATCTTCAACTATTTATTTAGTTAGTCACTTTAGGCTATTATTTGGGATGCCTTGCATTAGACTCGGGCGAGCTTTCATTTCGAGATTGCTGGGGCATGCGTATTTCCGTTGATAGCAAAGAGGATATTCTTTAAGCAAACTTACATTGTCGGTGTCGTGATCATTTGCGGGTTATAGGATAATGCCGGAAAAAGACGAGCGGGACTCTTTACAAATTGCACTTGTGAGAGGTTGTTACTACACGATCGCTGTTGGTTTTCGGATCCGAAGCTCAATTTGTTGAAGAGGCTCTGTACCAAGAATATTCCGATTCGAGGGTACAGTCGACTATACCAATTTTGCTCCTATTTTTCCGTTCTATTTTTGTGTACGCCCCTCTGATGATCCGGCTTAGTCGTTCCATGAAGAGAAATGTGTTCGGAAGTGGATACTCACTACCACAAATTGATCCTAATTCAAAAAGCAGTATGATTCCCATGAATTTCAATACATTAGAGGAATAGATTTTCGTTTCCATCCGGTACCCTCTGAGGGTATGAGGCCCCTTTTATGTGAACGCCTCCACCTGATACAGCTCTTATGGTTTAACAGTGGACAATATTATACAAATCATGCCCTCTTAACTTAGGCGGCGTGTCAATAGACATCCTCAAAATGAGCGTCTCCGGAACAAAAGAAGTTGTGGAGTCTCGAAAATGATGTAGGCCAAACAAGTGAATCTATTTTATTTTTTCCCAAAGGGTTTAATCCTAACACCAATCCGAATAAAGGGCGTCCCTAAGGTGATATTCTTTATTCAGAACGACTAAGGAGAAGGAGCGGAGTTCGTATGTGGAGGAGAGGCACTCTTCCGAAGACTCCCTATGTTGTCTTGTCATTCCCGACGCGCAGGAGGATGGTTTTTGAGGAGGTTGCGGAGTGAGTTACCACTAGTTGGCTAAATTTCATTCGGCGACGGCGTTAGGATCATAGGTTTCCATAAGTAAGAATAACATCTCCATTGGTATGAGGTCTTTGGAAAAGCCCTCGAAGCAAAAAAGCGCCATATGAGAGCCTTCCTAGCTCAGACCGTGGACACATCCATATGCAATGACCATGTGGAAGAATTTTTGAGTTCTTATACCTACTTCTAACTCTGTTGTTTTCATTGTCTGGTGCAAAATCGACAGGCGGTCTTTTTCCCGTCTTGATCGAATATTCTCCGTTCTCTGTTATTCTATTGCTGCTCCATGCTCTTCTTGTGTGGTTTTGCACTGGGATCTTTGAGATCTTTTAATGTTCCGGCCTGTTGTTGTAGAGTTGTCACTTGATTTTGTGAGTCAGTGTCTTTATAGCATTTAACTGGTAACTTCTCAAAATATTACAAAGTGTTGGGCTGATTTGCCTTGTGATTTGAGTATGGTTTCCTCTATATTTGGCTTGTTTATTCGCCTAACAAGGAAATTGAAAGCAGAGTAATTGTTGGAAAAGCATATACAATCCAGTTATATTGAAAACTTTGCACAAAACCCATCTCTGTTCTTCATGTTGTAAGGCGAAGCTGAACAGCTAAAACCTGTGTTGTTTTGGCTTTGTTTTCTGTAGGTATGGGATCTCGGTGGTCAAGAGAGGCTTAGGACATCATGGGCAACATATTACCGTGGAACTCATGCTGTGATTGCGGTGATAGACAGCACAGATAGAGCAAGAATCAGCATTATGAAGGACGAGCTCTTCAGACTACTCGGTCACGATGATCTTCAGCAATCTGTGGTGCTGGTTTTTGCAAACAAACAGGATCTTAAGGATGCCATGACTCCGGTCGAAATCACCGATGCTCTTTCGCTTCACAGCATCAAGAATCACGACTGGCACATCCAAGCCTGCTCTGCCCTCACCGGAGATGGTCTATACGACGGTCTTGGGTGGATTGCTCAGCAAGTTACCGGTAAAACGCCGAGTTGA

mRNA sequence

ATGGATCAAATTGATCCTTCTGCCAGTGTCCATCCACACTTTCGCGCTACGAAGCTCCTTAACCTTGCCGACCAGAAATATATCCCGCTGCCCCCCCAAAACTTCGGACAACTTATTACCTCACCTGAATTCTCGACGGATTTAGTGGATAATGCCGGAAAAAGACGAGCGGGACTCTTTACAAATTGCACTTGTGAGAGGTTGTTACTACACGATCGCTGTTGGTTTTCGGATCCGAAGCTCAATTTGTTGAAGAGGCTCTGTACCAAGAATATTCCGATTCGAGGTCGTTCCATGAAGAGAAATGTGTTCGGAAGTGGATACTCACTACCACAAATTGATCCTAATTCAAAAAGCAAACGACTAAGGAGAAGGAGCGGAGTTCGTATGTGGAGGAGAGGCACTCTTCCGAAGACTCCCTATGTTGTCTTGTCATTCCCGACGCGCAGGAGGATGGTTTTTGAGGAGGTATGGGATCTCGGTGGTCAAGAGAGGCTTAGGACATCATGGGCAACATATTACCGTGGAACTCATGCTGTGATTGCGGTGATAGACAGCACAGATAGAGCAAGAATCAGCATTATGAAGGACGAGCTCTTCAGACTACTCGGTCACGATGATCTTCAGCAATCTGTGGTGCTGGTTTTTGCAAACAAACAGGATCTTAAGGATGCCATGACTCCGGTCGAAATCACCGATGCTCTTTCGCTTCACAGCATCAAGAATCACGACTGGCACATCCAAGCCTGCTCTGCCCTCACCGGAGATGGTCTATACGACGGTCTTGGGTGGATTGCTCAGCAAGTTACCGGTAAAACGCCGAGTTGA

Coding sequence (CDS)

ATGGATCAAATTGATCCTTCTGCCAGTGTCCATCCACACTTTCGCGCTACGAAGCTCCTTAACCTTGCCGACCAGAAATATATCCCGCTGCCCCCCCAAAACTTCGGACAACTTATTACCTCACCTGAATTCTCGACGGATTTAGTGGATAATGCCGGAAAAAGACGAGCGGGACTCTTTACAAATTGCACTTGTGAGAGGTTGTTACTACACGATCGCTGTTGGTTTTCGGATCCGAAGCTCAATTTGTTGAAGAGGCTCTGTACCAAGAATATTCCGATTCGAGGTCGTTCCATGAAGAGAAATGTGTTCGGAAGTGGATACTCACTACCACAAATTGATCCTAATTCAAAAAGCAAACGACTAAGGAGAAGGAGCGGAGTTCGTATGTGGAGGAGAGGCACTCTTCCGAAGACTCCCTATGTTGTCTTGTCATTCCCGACGCGCAGGAGGATGGTTTTTGAGGAGGTATGGGATCTCGGTGGTCAAGAGAGGCTTAGGACATCATGGGCAACATATTACCGTGGAACTCATGCTGTGATTGCGGTGATAGACAGCACAGATAGAGCAAGAATCAGCATTATGAAGGACGAGCTCTTCAGACTACTCGGTCACGATGATCTTCAGCAATCTGTGGTGCTGGTTTTTGCAAACAAACAGGATCTTAAGGATGCCATGACTCCGGTCGAAATCACCGATGCTCTTTCGCTTCACAGCATCAAGAATCACGACTGGCACATCCAAGCCTGCTCTGCCCTCACCGGAGATGGTCTATACGACGGTCTTGGGTGGATTGCTCAGCAAGTTACCGGTAAAACGCCGAGTTGA

Protein sequence

MDQIDPSASVHPHFRATKLLNLADQKYIPLPPQNFGQLITSPEFSTDLVDNAGKRRAGLFTNCTCERLLLHDRCWFSDPKLNLLKRLCTKNIPIRGRSMKRNVFGSGYSLPQIDPNSKSKRLRRRSGVRMWRRGTLPKTPYVVLSFPTRRRMVFEEVWDLGGQERLRTSWATYYRGTHAVIAVIDSTDRARISIMKDELFRLLGHDDLQQSVVLVFANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQVTGKTPS
Homology
BLAST of Carg06181 vs. NCBI nr
Match: KAG7028866.1 (ADP-ribosylation factor-like protein 5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 572.8 bits (1475), Expect = 1.6e-159
Identity = 275/275 (100.00%), Postives = 275/275 (100.00%), Query Frame = 0

Query: 1   MDQIDPSASVHPHFRATKLLNLADQKYIPLPPQNFGQLITSPEFSTDLVDNAGKRRAGLF 60
           MDQIDPSASVHPHFRATKLLNLADQKYIPLPPQNFGQLITSPEFSTDLVDNAGKRRAGLF
Sbjct: 1   MDQIDPSASVHPHFRATKLLNLADQKYIPLPPQNFGQLITSPEFSTDLVDNAGKRRAGLF 60

Query: 61  TNCTCERLLLHDRCWFSDPKLNLLKRLCTKNIPIRGRSMKRNVFGSGYSLPQIDPNSKSK 120
           TNCTCERLLLHDRCWFSDPKLNLLKRLCTKNIPIRGRSMKRNVFGSGYSLPQIDPNSKSK
Sbjct: 61  TNCTCERLLLHDRCWFSDPKLNLLKRLCTKNIPIRGRSMKRNVFGSGYSLPQIDPNSKSK 120

Query: 121 RLRRRSGVRMWRRGTLPKTPYVVLSFPTRRRMVFEEVWDLGGQERLRTSWATYYRGTHAV 180
           RLRRRSGVRMWRRGTLPKTPYVVLSFPTRRRMVFEEVWDLGGQERLRTSWATYYRGTHAV
Sbjct: 121 RLRRRSGVRMWRRGTLPKTPYVVLSFPTRRRMVFEEVWDLGGQERLRTSWATYYRGTHAV 180

Query: 181 IAVIDSTDRARISIMKDELFRLLGHDDLQQSVVLVFANKQDLKDAMTPVEITDALSLHSI 240
           IAVIDSTDRARISIMKDELFRLLGHDDLQQSVVLVFANKQDLKDAMTPVEITDALSLHSI
Sbjct: 181 IAVIDSTDRARISIMKDELFRLLGHDDLQQSVVLVFANKQDLKDAMTPVEITDALSLHSI 240

Query: 241 KNHDWHIQACSALTGDGLYDGLGWIAQQVTGKTPS 276
           KNHDWHIQACSALTGDGLYDGLGWIAQQVTGKTPS
Sbjct: 241 KNHDWHIQACSALTGDGLYDGLGWIAQQVTGKTPS 275

BLAST of Carg06181 vs. NCBI nr
Match: XP_022937092.1 (ADP-ribosylation factor-like protein 5 [Cucurbita moschata] >XP_022937100.1 ADP-ribosylation factor-like protein 5 [Cucurbita moschata] >XP_022937107.1 ADP-ribosylation factor-like protein 5 [Cucurbita moschata] >XP_022937116.1 ADP-ribosylation factor-like protein 5 [Cucurbita moschata] >XP_023538887.1 ADP-ribosylation factor-like protein 5 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 250.4 bits (638), Expect = 1.8e-62
Identity = 120/120 (100.00%), Postives = 120/120 (100.00%), Query Frame = 0

Query: 156 EVWDLGGQERLRTSWATYYRGTHAVIAVIDSTDRARISIMKDELFRLLGHDDLQQSVVLV 215
           EVWDLGGQERLRTSWATYYRGTHAVIAVIDSTDRARISIMKDELFRLLGHDDLQQSVVLV
Sbjct: 64  EVWDLGGQERLRTSWATYYRGTHAVIAVIDSTDRARISIMKDELFRLLGHDDLQQSVVLV 123

Query: 216 FANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQVTGKTPS 275
           FANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQVTGKTPS
Sbjct: 124 FANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQVTGKTPS 183

BLAST of Carg06181 vs. NCBI nr
Match: KAG6597406.1 (ADP-ribosylation factor-like protein 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 250.4 bits (638), Expect = 1.8e-62
Identity = 120/120 (100.00%), Postives = 120/120 (100.00%), Query Frame = 0

Query: 156 EVWDLGGQERLRTSWATYYRGTHAVIAVIDSTDRARISIMKDELFRLLGHDDLQQSVVLV 215
           EVWDLGGQERLRTSWATYYRGTHAVIAVIDSTDRARISIMKDELFRLLGHDDLQQSVVLV
Sbjct: 54  EVWDLGGQERLRTSWATYYRGTHAVIAVIDSTDRARISIMKDELFRLLGHDDLQQSVVLV 113

Query: 216 FANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQVTGKTPS 275
           FANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQVTGKTPS
Sbjct: 114 FANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQVTGKTPS 173

BLAST of Carg06181 vs. NCBI nr
Match: XP_023538886.1 (ADP-ribosylation factor-like protein 5 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 250.4 bits (638), Expect = 1.8e-62
Identity = 120/120 (100.00%), Postives = 120/120 (100.00%), Query Frame = 0

Query: 156 EVWDLGGQERLRTSWATYYRGTHAVIAVIDSTDRARISIMKDELFRLLGHDDLQQSVVLV 215
           EVWDLGGQERLRTSWATYYRGTHAVIAVIDSTDRARISIMKDELFRLLGHDDLQQSVVLV
Sbjct: 149 EVWDLGGQERLRTSWATYYRGTHAVIAVIDSTDRARISIMKDELFRLLGHDDLQQSVVLV 208

Query: 216 FANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQVTGKTPS 275
           FANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQVTGKTPS
Sbjct: 209 FANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQVTGKTPS 268

BLAST of Carg06181 vs. NCBI nr
Match: XP_008438599.1 (PREDICTED: ADP-ribosylation factor-like protein 5 [Cucumis melo])

HSP 1 Score: 244.6 bits (623), Expect = 1.0e-60
Identity = 116/120 (96.67%), Postives = 119/120 (99.17%), Query Frame = 0

Query: 156 EVWDLGGQERLRTSWATYYRGTHAVIAVIDSTDRARISIMKDELFRLLGHDDLQQSVVLV 215
           EVWDLGGQERLRTSWATYYRGTHAVIAVIDSTDRARI+IMKDELFRLLGH+DLQQ+VVLV
Sbjct: 64  EVWDLGGQERLRTSWATYYRGTHAVIAVIDSTDRARITIMKDELFRLLGHEDLQQAVVLV 123

Query: 216 FANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQVTGKTPS 275
           FANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQVTGK PS
Sbjct: 124 FANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQVTGKAPS 183

BLAST of Carg06181 vs. ExPASy Swiss-Prot
Match: Q55AD9 (ADP-ribosylation factor-like protein 5 OS=Dictyostelium discoideum OX=44689 GN=arl5 PE=3 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 4.3e-38
Identity = 68/113 (60.18%), Postives = 94/113 (83.19%), Query Frame = 0

Query: 157 VWDLGGQERLRTSWATYYRGTHAVIAVIDSTDRARISIMKDELFRLLGHDDLQQSVVLVF 216
           +WDLGGQ+ LR++W  YY  T AVI VIDSTDRAR++++K+ELF++L H++L++S++L++
Sbjct: 68  MWDLGGQDLLRSTWNQYYINTQAVILVIDSTDRARVNLIKEELFKMLAHENLKKSIILIY 127

Query: 217 ANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQV 270
           ANKQDLKDAM+P E++  LSLHSIK+HD+HIQAC ALTG GL  GL W+   +
Sbjct: 128 ANKQDLKDAMSPTELSTLLSLHSIKDHDYHIQACCALTGQGLESGLDWLVSHI 180

BLAST of Carg06181 vs. ExPASy Swiss-Prot
Match: Q9Y689 (ADP-ribosylation factor-like protein 5A OS=Homo sapiens OX=9606 GN=ARL5A PE=1 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 1.7e-34
Identity = 64/113 (56.64%), Postives = 88/113 (77.88%), Query Frame = 0

Query: 157 VWDLGGQERLRTSWATYYRGTHAVIAVIDSTDRARISIMKDELFRLLGHDDLQQSVVLVF 216
           +WD+GGQE LR+SW TYY  T  VI V+DSTDR RIS+ ++EL+++L H+DL+++ +L+F
Sbjct: 64  MWDIGGQESLRSSWNTYYTNTEFVIVVVDSTDRERISVTREELYKMLAHEDLRKAGLLIF 123

Query: 217 ANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQV 270
           ANKQD+K+ MT  EI+  L L SIK+H WHIQAC ALTG+GL  GL W+  ++
Sbjct: 124 ANKQDVKECMTVAEISQFLKLTSIKDHQWHIQACCALTGEGLCQGLEWMMSRL 176

BLAST of Carg06181 vs. ExPASy Swiss-Prot
Match: P51646 (ADP-ribosylation factor-like protein 5A OS=Rattus norvegicus OX=10116 GN=Arl5a PE=2 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 1.7e-34
Identity = 64/113 (56.64%), Postives = 88/113 (77.88%), Query Frame = 0

Query: 157 VWDLGGQERLRTSWATYYRGTHAVIAVIDSTDRARISIMKDELFRLLGHDDLQQSVVLVF 216
           +WD+GGQE LR+SW TYY  T  VI V+DSTDR RIS+ ++EL+++L H+DL+++ +L+F
Sbjct: 64  MWDIGGQESLRSSWNTYYTNTEFVIVVVDSTDRERISVTREELYKMLAHEDLRKAGLLIF 123

Query: 217 ANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQV 270
           ANKQD+K+ MT  EI+  L L SIK+H WHIQAC ALTG+GL  GL W+  ++
Sbjct: 124 ANKQDVKECMTVAEISQFLKLTSIKDHQWHIQACCALTGEGLCQGLEWMMSRL 176

BLAST of Carg06181 vs. ExPASy Swiss-Prot
Match: Q96KC2 (ADP-ribosylation factor-like protein 5B OS=Homo sapiens OX=9606 GN=ARL5B PE=1 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 2.2e-34
Identity = 63/113 (55.75%), Postives = 87/113 (76.99%), Query Frame = 0

Query: 157 VWDLGGQERLRTSWATYYRGTHAVIAVIDSTDRARISIMKDELFRLLGHDDLQQSVVLVF 216
           +WD+GGQE LR+SW TYY  T  +I V+DS DR R++I K+EL+R+L H+DL+++ VL+F
Sbjct: 64  MWDIGGQESLRSSWNTYYSNTEFIILVVDSIDRERLAITKEELYRMLAHEDLRKAAVLIF 123

Query: 217 ANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQV 270
           ANKQD+K  MT  EI+  L+L SIK+H WHIQ+C ALTG+GL  GL W+  ++
Sbjct: 124 ANKQDMKGCMTAAEISKYLTLSSIKDHPWHIQSCCALTGEGLCQGLEWMTSRI 176

BLAST of Carg06181 vs. ExPASy Swiss-Prot
Match: Q9D4P0 (ADP-ribosylation factor-like protein 5B OS=Mus musculus OX=10090 GN=Arl5b PE=2 SV=3)

HSP 1 Score: 147.5 bits (371), Expect = 2.2e-34
Identity = 63/113 (55.75%), Postives = 87/113 (76.99%), Query Frame = 0

Query: 157 VWDLGGQERLRTSWATYYRGTHAVIAVIDSTDRARISIMKDELFRLLGHDDLQQSVVLVF 216
           +WD+GGQE LR+SW TYY  T  +I V+DS DR R++I K+EL+R+L H+DL+++ VL+F
Sbjct: 64  MWDIGGQESLRSSWNTYYSNTEFIILVVDSIDRERLAITKEELYRMLAHEDLRKAAVLIF 123

Query: 217 ANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQV 270
           ANKQD+K  MT  EI+  L+L SIK+H WHIQ+C ALTG+GL  GL W+  ++
Sbjct: 124 ANKQDMKGCMTAAEISKYLTLSSIKDHPWHIQSCCALTGEGLCQGLEWMTSRI 176

BLAST of Carg06181 vs. ExPASy TrEMBL
Match: A0A6J1FA74 (ADP-ribosylation factor-like protein 5 OS=Cucurbita moschata OX=3662 GN=LOC111443495 PE=3 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 8.9e-63
Identity = 120/120 (100.00%), Postives = 120/120 (100.00%), Query Frame = 0

Query: 156 EVWDLGGQERLRTSWATYYRGTHAVIAVIDSTDRARISIMKDELFRLLGHDDLQQSVVLV 215
           EVWDLGGQERLRTSWATYYRGTHAVIAVIDSTDRARISIMKDELFRLLGHDDLQQSVVLV
Sbjct: 64  EVWDLGGQERLRTSWATYYRGTHAVIAVIDSTDRARISIMKDELFRLLGHDDLQQSVVLV 123

Query: 216 FANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQVTGKTPS 275
           FANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQVTGKTPS
Sbjct: 124 FANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQVTGKTPS 183

BLAST of Carg06181 vs. ExPASy TrEMBL
Match: A0A5A7U254 (ADP-ribosylation factor-like protein 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00910 PE=3 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 4.9e-61
Identity = 116/120 (96.67%), Postives = 119/120 (99.17%), Query Frame = 0

Query: 156 EVWDLGGQERLRTSWATYYRGTHAVIAVIDSTDRARISIMKDELFRLLGHDDLQQSVVLV 215
           EVWDLGGQERLRTSWATYYRGTHAVIAVIDSTDRARI+IMKDELFRLLGH+DLQQ+VVLV
Sbjct: 126 EVWDLGGQERLRTSWATYYRGTHAVIAVIDSTDRARITIMKDELFRLLGHEDLQQAVVLV 185

Query: 216 FANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQVTGKTPS 275
           FANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQVTGK PS
Sbjct: 186 FANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQVTGKAPS 245

BLAST of Carg06181 vs. ExPASy TrEMBL
Match: A0A1S3AWS5 (ADP-ribosylation factor-like protein 5 OS=Cucumis melo OX=3656 GN=LOC103483660 PE=3 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 4.9e-61
Identity = 116/120 (96.67%), Postives = 119/120 (99.17%), Query Frame = 0

Query: 156 EVWDLGGQERLRTSWATYYRGTHAVIAVIDSTDRARISIMKDELFRLLGHDDLQQSVVLV 215
           EVWDLGGQERLRTSWATYYRGTHAVIAVIDSTDRARI+IMKDELFRLLGH+DLQQ+VVLV
Sbjct: 64  EVWDLGGQERLRTSWATYYRGTHAVIAVIDSTDRARITIMKDELFRLLGHEDLQQAVVLV 123

Query: 216 FANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQVTGKTPS 275
           FANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQVTGK PS
Sbjct: 124 FANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQVTGKAPS 183

BLAST of Carg06181 vs. ExPASy TrEMBL
Match: A0A6J1IE39 (ADP-ribosylation factor-like protein 5 OS=Cucurbita maxima OX=3661 GN=LOC111474731 PE=3 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 8.3e-61
Identity = 117/120 (97.50%), Postives = 118/120 (98.33%), Query Frame = 0

Query: 156 EVWDLGGQERLRTSWATYYRGTHAVIAVIDSTDRARISIMKDELFRLLGHDDLQQSVVLV 215
           EVWDLGGQERLRTSWATYYRGTHAVIAVI+STDRARISIMKDELFRLLGHDDLQQSVVLV
Sbjct: 64  EVWDLGGQERLRTSWATYYRGTHAVIAVINSTDRARISIMKDELFRLLGHDDLQQSVVLV 123

Query: 216 FANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQVTGKTPS 275
           FANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQVT K PS
Sbjct: 124 FANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQVTSKMPS 183

BLAST of Carg06181 vs. ExPASy TrEMBL
Match: A0A6J1IER4 (ADP-ribosylation factor-like protein 5 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111475364 PE=3 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 8.3e-61
Identity = 117/120 (97.50%), Postives = 118/120 (98.33%), Query Frame = 0

Query: 156 EVWDLGGQERLRTSWATYYRGTHAVIAVIDSTDRARISIMKDELFRLLGHDDLQQSVVLV 215
           EVWDLGGQERLRTSWATYYRGTHAVIAVI+STDRARISIMKDELFRLLGHDDLQQSVVLV
Sbjct: 54  EVWDLGGQERLRTSWATYYRGTHAVIAVINSTDRARISIMKDELFRLLGHDDLQQSVVLV 113

Query: 216 FANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQVTGKTPS 275
           FANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQVT K PS
Sbjct: 114 FANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQVTSKMPS 173

BLAST of Carg06181 vs. TAIR 10
Match: AT3G22950.1 (ADP-ribosylation factor C1 )

HSP 1 Score: 221.5 bits (563), Expect = 8.5e-58
Identity = 104/120 (86.67%), Postives = 111/120 (92.50%), Query Frame = 0

Query: 156 EVWDLGGQERLRTSWATYYRGTHAVIAVIDSTDRARISIMKDELFRLLGHDDLQQSVVLV 215
           EVWDLGGQ+RLRTSWATYYRGTHAVI VIDSTDRARIS MKDEL RLLGH+DLQ SV+LV
Sbjct: 64  EVWDLGGQDRLRTSWATYYRGTHAVIVVIDSTDRARISFMKDELARLLGHEDLQNSVILV 123

Query: 216 FANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQVTGKTPS 275
           FANKQDLKDAMTP EITDAL+LHSIKNHDWHIQA  A+TG+GLYDGLGWIAQ+VTGK  S
Sbjct: 124 FANKQDLKDAMTPAEITDALNLHSIKNHDWHIQASCAVTGEGLYDGLGWIAQKVTGKATS 183

BLAST of Carg06181 vs. TAIR 10
Match: AT3G22950.2 (ADP-ribosylation factor C1 )

HSP 1 Score: 221.5 bits (563), Expect = 8.5e-58
Identity = 104/120 (86.67%), Postives = 111/120 (92.50%), Query Frame = 0

Query: 156 EVWDLGGQERLRTSWATYYRGTHAVIAVIDSTDRARISIMKDELFRLLGHDDLQQSVVLV 215
           EVWDLGGQ+RLRTSWATYYRGTHAVI VIDSTDRARIS MKDEL RLLGH+DLQ SV+LV
Sbjct: 64  EVWDLGGQDRLRTSWATYYRGTHAVIVVIDSTDRARISFMKDELARLLGHEDLQNSVILV 123

Query: 216 FANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQVTGKTPS 275
           FANKQDLKDAMTP EITDAL+LHSIKNHDWHIQA  A+TG+GLYDGLGWIAQ+VTGK  S
Sbjct: 124 FANKQDLKDAMTPAEITDALNLHSIKNHDWHIQASCAVTGEGLYDGLGWIAQKVTGKATS 183

BLAST of Carg06181 vs. TAIR 10
Match: AT1G70490.1 (Ras-related small GTP-binding family protein )

HSP 1 Score: 134.4 bits (337), Expect = 1.4e-31
Identity = 57/116 (49.14%), Postives = 83/116 (71.55%), Query Frame = 0

Query: 157 VWDLGGQERLRTSWATYYRGTHAVIAVIDSTDRARISIMKDELFRLLGHDDLQQSVVLVF 216
           VWD+GGQ+++R  W  Y++ T  +I V+DS DR R+   +DEL R+L  D+L+ +V+LVF
Sbjct: 65  VWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVF 124

Query: 217 ANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQVTGK 273
           ANKQDL +AM   EITD L LHS++   W+IQ+  A +G+GLY+GL W++  + GK
Sbjct: 125 ANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSNNIAGK 180

BLAST of Carg06181 vs. TAIR 10
Match: AT1G23490.1 (ADP-ribosylation factor 1 )

HSP 1 Score: 134.4 bits (337), Expect = 1.4e-31
Identity = 57/116 (49.14%), Postives = 83/116 (71.55%), Query Frame = 0

Query: 157 VWDLGGQERLRTSWATYYRGTHAVIAVIDSTDRARISIMKDELFRLLGHDDLQQSVVLVF 216
           VWD+GGQ+++R  W  Y++ T  +I V+DS DR R+   +DEL R+L  D+L+ +V+LVF
Sbjct: 65  VWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVF 124

Query: 217 ANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQVTGK 273
           ANKQDL +AM   EITD L LHS++   W+IQ+  A +G+GLY+GL W++  + GK
Sbjct: 125 ANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSNNIAGK 180

BLAST of Carg06181 vs. TAIR 10
Match: AT1G70490.3 (Ras-related small GTP-binding family protein )

HSP 1 Score: 134.4 bits (337), Expect = 1.4e-31
Identity = 57/116 (49.14%), Postives = 83/116 (71.55%), Query Frame = 0

Query: 157 VWDLGGQERLRTSWATYYRGTHAVIAVIDSTDRARISIMKDELFRLLGHDDLQQSVVLVF 216
           VWD+GGQ+++R  W  Y++ T  +I V+DS DR R+   +DEL R+L  D+L+ +V+LVF
Sbjct: 65  VWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVF 124

Query: 217 ANKQDLKDAMTPVEITDALSLHSIKNHDWHIQACSALTGDGLYDGLGWIAQQVTGK 273
           ANKQDL +AM   EITD L LHS++   W+IQ+  A +G+GLY+GL W++  + GK
Sbjct: 125 ANKQDLPNAMNAAEITDKLGLHSLRQRHWYIQSTCATSGEGLYEGLDWLSNNIAGK 180

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7028866.11.6e-159100.00ADP-ribosylation factor-like protein 5, partial [Cucurbita argyrosperma subsp. a... [more]
XP_022937092.11.8e-62100.00ADP-ribosylation factor-like protein 5 [Cucurbita moschata] >XP_022937100.1 ADP-... [more]
KAG6597406.11.8e-62100.00ADP-ribosylation factor-like protein 5, partial [Cucurbita argyrosperma subsp. s... [more]
XP_023538886.11.8e-62100.00ADP-ribosylation factor-like protein 5 isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_008438599.11.0e-6096.67PREDICTED: ADP-ribosylation factor-like protein 5 [Cucumis melo][more]
Match NameE-valueIdentityDescription
Q55AD94.3e-3860.18ADP-ribosylation factor-like protein 5 OS=Dictyostelium discoideum OX=44689 GN=a... [more]
Q9Y6891.7e-3456.64ADP-ribosylation factor-like protein 5A OS=Homo sapiens OX=9606 GN=ARL5A PE=1 SV... [more]
P516461.7e-3456.64ADP-ribosylation factor-like protein 5A OS=Rattus norvegicus OX=10116 GN=Arl5a P... [more]
Q96KC22.2e-3455.75ADP-ribosylation factor-like protein 5B OS=Homo sapiens OX=9606 GN=ARL5B PE=1 SV... [more]
Q9D4P02.2e-3455.75ADP-ribosylation factor-like protein 5B OS=Mus musculus OX=10090 GN=Arl5b PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A6J1FA748.9e-63100.00ADP-ribosylation factor-like protein 5 OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
A0A5A7U2544.9e-6196.67ADP-ribosylation factor-like protein 5 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3AWS54.9e-6196.67ADP-ribosylation factor-like protein 5 OS=Cucumis melo OX=3656 GN=LOC103483660 P... [more]
A0A6J1IE398.3e-6197.50ADP-ribosylation factor-like protein 5 OS=Cucurbita maxima OX=3661 GN=LOC1114747... [more]
A0A6J1IER48.3e-6197.50ADP-ribosylation factor-like protein 5 isoform X1 OS=Cucurbita maxima OX=3661 GN... [more]
Match NameE-valueIdentityDescription
AT3G22950.18.5e-5886.67ADP-ribosylation factor C1 [more]
AT3G22950.28.5e-5886.67ADP-ribosylation factor C1 [more]
AT1G70490.11.4e-3149.14Ras-related small GTP-binding family protein [more]
AT1G23490.11.4e-3149.14ADP-ribosylation factor 1 [more]
AT1G70490.31.4e-3149.14Ras-related small GTP-binding family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSMARTSM00177arf_sub_2coord: 92..273
e-value: 3.4E-34
score: 129.6
NoneNo IPR availablePANTHERPTHR11711:SF369ADP-RIBOSYLATION FACTOR C1coord: 155..275
NoneNo IPR availablePANTHERPTHR11711ADP RIBOSYLATION FACTOR-RELATEDcoord: 155..275
NoneNo IPR availablePROSITEPS51417ARFcoord: 1..269
score: 15.71002
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 134..272
e-value: 2.0E-43
score: 150.0
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 155..271
IPR006689Small GTPase superfamily, ARF/SAR typePFAMPF00025Arfcoord: 156..268
e-value: 2.3E-39
score: 134.7

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg06181-RACarg06181-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006886 intracellular protein transport
biological_process GO:1903292 protein localization to Golgi membrane
biological_process GO:0016192 vesicle-mediated transport
cellular_component GO:0005802 trans-Golgi network
molecular_function GO:0003924 GTPase activity
molecular_function GO:0005525 GTP binding