Carg06035 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg06035
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Phytocyanin domain containing protein
Location	Carg_Chr04: 21614919 .. 21615506 (-)
RNA-Seq Expression	Carg06035
Synteny	Carg06035

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGGATTAGGGAGAGGCTTGTTTGTTGTTATAGCTGCTGCAGCTTCAATGCTGGAAATGGGTTTGGCTGATCAAAGACATATGGTTGGGGGAACCCAAGGTTGGCAACAATCCATTGATTTCGACTCTTGGGCGGAAGCTCAGACCTTCAAAGTTGGCGACGAACTCGGTATATCTCTCTTCATCATCTTTTCAATTCCAAACCATATTTCATTGCATATTTGTCTTCATATGTGTAACAAAATTTGGACAGTTTTCAACTACGCTTCGGGGCTGCACAGTATGGTAGAGCTTCCGGACGAGAGTGCGTATGAGAACTGCGATATTGGGAGCGCCATCGAGACGAAGAGCAGCGGGAAGGATGGCATCAAGTTGACGAAGCCGGGAACACGATACTTCGCGTGCGGCACCATGGGGCATTGCAGCCAAGGCATGAAGGTCAAGATCAAAATAGCCACTGGCACAGCTTCCTCTACTCCATCTCATCCTTCTTCTTCTTCATCTTCTTCATCTTCTTCGCATTATTCTCTTCTGGGTTGCTTCCTCATCATGGCTTCCTACGCCTTGTTGAGGTTCATGTTATGA

mRNA sequence

ATGGAGGGATTAGGGAGAGGCTTGTTTGTTGTTATAGCTGCTGCAGCTTCAATGCTGGAAATGGGTTTGGCTGATCAAAGACATATGGTTGGGGGAACCCAAGGTTGGCAACAATCCATTGATTTCGACTCTTGGGCGGAAGCTCAGACCTTCAAAGTTGGCGACGAACTCGTTTTCAACTACGCTTCGGGGCTGCACAGTATGGTAGAGCTTCCGGACGAGAGTGCGTATGAGAACTGCGATATTGGGAGCGCCATCGAGACGAAGAGCAGCGGGAAGGATGGCATCAAGTTGACGAAGCCGGGAACACGATACTTCGCGTGCGGCACCATGGGGCATTGCAGCCAAGGCATGAAGGTCAAGATCAAAATAGCCACTGGCACAGCTTCCTCTACTCCATCTCATCCTTCTTCTTCTTCATCTTCTTCATCTTCTTCGCATTATTCTCTTCTGGGTTGCTTCCTCATCATGGCTTCCTACGCCTTGTTGAGGTTCATGTTATGA

Coding sequence (CDS)

ATGGAGGGATTAGGGAGAGGCTTGTTTGTTGTTATAGCTGCTGCAGCTTCAATGCTGGAAATGGGTTTGGCTGATCAAAGACATATGGTTGGGGGAACCCAAGGTTGGCAACAATCCATTGATTTCGACTCTTGGGCGGAAGCTCAGACCTTCAAAGTTGGCGACGAACTCGTTTTCAACTACGCTTCGGGGCTGCACAGTATGGTAGAGCTTCCGGACGAGAGTGCGTATGAGAACTGCGATATTGGGAGCGCCATCGAGACGAAGAGCAGCGGGAAGGATGGCATCAAGTTGACGAAGCCGGGAACACGATACTTCGCGTGCGGCACCATGGGGCATTGCAGCCAAGGCATGAAGGTCAAGATCAAAATAGCCACTGGCACAGCTTCCTCTACTCCATCTCATCCTTCTTCTTCTTCATCTTCTTCATCTTCTTCGCATTATTCTCTTCTGGGTTGCTTCCTCATCATGGCTTCCTACGCCTTGTTGAGGTTCATGTTATGA

Protein sequence

MEGLGRGLFVVIAAAASMLEMGLADQRHMVGGTQGWQQSIDFDSWAEAQTFKVGDELVFNYASGLHSMVELPDESAYENCDIGSAIETKSSGKDGIKLTKPGTRYFACGTMGHCSQGMKVKIKIATGTASSTPSHPSSSSSSSSSSHYSLLGCFLIMASYALLRFML

Homology

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BLAST of Carg06035 vs. NCBI nr
Match: KAG7033356.1 (Uclacyanin 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 324.7 bits (831), Expect = 4.6e-85
Identity = 167/167 (100.00%), Postives = 167/167 (100.00%), Query Frame = 0


Query: 1   MEGLGRGLFVVIAAAASMLEMGLADQRHMVGGTQGWQQSIDFDSWAEAQTFKVGDELVFN 60
           MEGLGRGLFVVIAAAASMLEMGLADQRHMVGGTQGWQQSIDFDSWAEAQTFKVGDELVFN
Sbjct: 1   MEGLGRGLFVVIAAAASMLEMGLADQRHMVGGTQGWQQSIDFDSWAEAQTFKVGDELVFN 60

Query: 61  YASGLHSMVELPDESAYENCDIGSAIETKSSGKDGIKLTKPGTRYFACGTMGHCSQGMKV 120
           YASGLHSMVELPDESAYENCDIGSAIETKSSGKDGIKLTKPGTRYFACGTMGHCSQGMKV
Sbjct: 61  YASGLHSMVELPDESAYENCDIGSAIETKSSGKDGIKLTKPGTRYFACGTMGHCSQGMKV 120

Query: 121 KIKIATGTASSTPSHPSSSSSSSSSSHYSLLGCFLIMASYALLRFML 168
           KIKIATGTASSTPSHPSSSSSSSSSSHYSLLGCFLIMASYALLRFML
Sbjct: 121 KIKIATGTASSTPSHPSSSSSSSSSSHYSLLGCFLIMASYALLRFML 167

[Close]

BLAST of Carg06035 vs. NCBI nr
Match: KAG6602670.1 (Uclacyanin 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 317.4 bits (812), Expect = 7.4e-83
Identity = 165/167 (98.80%), Postives = 166/167 (99.40%), Query Frame = 0


Query: 1   MEGLGRGLFVVIAAAASMLEMGLADQRHMVGGTQGWQQSIDFDSWAEAQTFKVGDELVFN 60
           MEGLGRGLFVVIAAAASMLEMGLADQRHMVGGTQGWQQSIDFDSWAEAQTFKVGDELVFN
Sbjct: 1   MEGLGRGLFVVIAAAASMLEMGLADQRHMVGGTQGWQQSIDFDSWAEAQTFKVGDELVFN 60

Query: 61  YASGLHSMVELPDESAYENCDIGSAIETKSSGKDGIKLTKPGTRYFACGTMGHCSQGMKV 120
           YASGLHS+VELPDESAYENCDIGSAIETKSSGKDGIKLTKPGTRYFACGTMGHCSQGMKV
Sbjct: 61  YASGLHSVVELPDESAYENCDIGSAIETKSSGKDGIKLTKPGTRYFACGTMGHCSQGMKV 120

Query: 121 KIKIATGTASSTPSHPSSSSSSSSSSHYSLLGCFLIMASYALLRFML 168
           KIKIATGTASSTPSHPSSSSSSSSSSHYSLLGCFLIMASYA LRFML
Sbjct: 121 KIKIATGTASSTPSHPSSSSSSSSSSHYSLLGCFLIMASYA-LRFML 166

[Close]

BLAST of Carg06035 vs. NCBI nr
Match: XP_022955584.1 (mavicyanin [Cucurbita moschata])

HSP 1 Score: 308.9 bits (790), Expect = 2.6e-80
Identity = 162/167 (97.01%), Postives = 163/167 (97.60%), Query Frame = 0


Query: 1   MEGLGRGLFVVIAAAASMLEMGLADQRHMVGGTQGWQQSIDFDSWAEAQTFKVGDELVFN 60
           MEGLGRGLFVVIAAAASMLEMGLADQRHMVGGTQGWQQSIDFDSWAEAQTFKVGDELVFN
Sbjct: 1   MEGLGRGLFVVIAAAASMLEMGLADQRHMVGGTQGWQQSIDFDSWAEAQTFKVGDELVFN 60

Query: 61  YASGLHSMVELPDESAYENCDIGSAIETKSSGKDGIKLTKPGTRYFACGTMGHCSQGMKV 120
           YASGLHS+VELPDESAYENCDIGSAIETKSSGKDGIKLTKPGTRYFACGTMGHCSQGMKV
Sbjct: 61  YASGLHSVVELPDESAYENCDIGSAIETKSSGKDGIKLTKPGTRYFACGTMGHCSQGMKV 120

Query: 121 KIKIATGTASSTPSHPSSSSSSSSSSHYSLLGCFLIMASYALLRFML 168
           KIKIATGTASSTPSHP   SSSSSSSHYSLLGCFLIMASYA LRFML
Sbjct: 121 KIKIATGTASSTPSHP---SSSSSSSHYSLLGCFLIMASYA-LRFML 163

[Close]

BLAST of Carg06035 vs. NCBI nr
Match: XP_022991129.1 (mavicyanin [Cucurbita maxima])

HSP 1 Score: 303.5 bits (776), Expect = 1.1e-78
Identity = 157/166 (94.58%), Postives = 162/166 (97.59%), Query Frame = 0


Query: 1   MEGLGRGLFVVIAAAASMLEMGLADQRHMVGGTQGWQQSIDFDSWAEAQTFKVGDELVFN 60
           MEGLGRGLFVVIAAAASMLEMGLADQRH+VGGTQGWQQSIDFDSWAEAQTFKVGD+LVFN
Sbjct: 1   MEGLGRGLFVVIAAAASMLEMGLADQRHVVGGTQGWQQSIDFDSWAEAQTFKVGDQLVFN 60

Query: 61  YASGLHSMVELPDESAYENCDIGSAIETKSSGKDGIKLTKPGTRYFACGTMGHCSQGMKV 120
           YASGLHS+VELPDESAYENCDIGSAIE+KSSGKDGIKLTK GTRYFACGTMGHCSQGMKV
Sbjct: 61  YASGLHSVVELPDESAYENCDIGSAIESKSSGKDGIKLTKAGTRYFACGTMGHCSQGMKV 120

Query: 121 KIKIATGTASSTPSHPSSSSSSSSSSHYSLLGCFLIMASYALLRFM 167
           KIKIATGTASSTPSHPSSS+SSS SSHYSLLGCFLIMA YA LRFM
Sbjct: 121 KIKIATGTASSTPSHPSSSASSSFSSHYSLLGCFLIMAFYA-LRFM 165

[Close]

BLAST of Carg06035 vs. NCBI nr
Match: XP_008441346.1 (PREDICTED: mavicyanin-like [Cucumis melo])

HSP 1 Score: 245.7 bits (626), Expect = 2.7e-61
Identity = 124/162 (76.54%), Postives = 145/162 (89.51%), Query Frame = 0


Query: 6   RGLFV-VIAAAASMLEMGLADQRHMVGGTQGWQQSIDFDSWAEAQTFKVGDELVFNYASG 65
           RG+FV +I  AA M EMGLADQRHMVGG+QGWQ+S+DFDSWA +QTFKVGD++VF Y S 
Sbjct: 11  RGVFVMMIVGAALMAEMGLADQRHMVGGSQGWQESVDFDSWASSQTFKVGDQIVFKYDSS 70

Query: 66  LHSMVELPDESAYENCDIGSAIETKSSGKDGIKLTKPGTRYFACGTMGHCSQGMKVKIKI 125
           LHS+VELP+ES+Y+NCDIG++IE+KSSG D IKLTK GTRYFACGT+GHCSQGMKVKIKI
Sbjct: 71  LHSVVELPEESSYKNCDIGNSIESKSSGNDVIKLTKSGTRYFACGTIGHCSQGMKVKIKI 130

Query: 126 ATGTASSTPSHPSSSSSSSSSSHYSLLGCFLIMASYALLRFM 167
           ATGTASSTPS PSSSSSSSSSSHYSL+G FL++  + +LRFM
Sbjct: 131 ATGTASSTPSSPSSSSSSSSSSHYSLMGFFLMLLPFYVLRFM 172

[Close]

BLAST of Carg06035 vs. ExPASy Swiss-Prot
Match: Q41001 (Blue copper protein OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 9.3e-20
Identity = 53/121 (43.80%), Postives = 76/121 (62.81%), Query Frame = 0


Query: 28  HMVGGTQGWQQSIDFDSWAEAQTFKVGDELVFNYASGLHSMVELPDESAYENCDIGSAIE 87
           + VG T GW    D+ +WA  +TF VGD LVFNY +G H++ E+  ES Y++C  G++I 
Sbjct: 27  YTVGDTSGWVIGGDYSTWASDKTFAVGDSLVFNYGAGAHTVDEV-KESDYKSCTSGNSIS 86

Query: 88  TKSSGKDGIKLTKPGTRYFACGTMGHCSQGMK--VKIKIATGTASSTPSHPSSSSSSSSS 147
           T S+G   I L K G  YF CG  GH + GMK  +K+K ++G++++  + PSSS   S S
Sbjct: 87  TDSTGATTIPLKKAGKHYFICGVPGHSTGGMKLSIKVKASSGSSAAPSATPSSSGKGSPS 146

[Close]

BLAST of Carg06035 vs. ExPASy Swiss-Prot
Match: O82081 (Uclacyanin 1 OS=Arabidopsis thaliana OX=3702 GN=UCC1 PE=1 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 3.9e-18
Identity = 52/147 (35.37%), Postives = 81/147 (55.10%), Query Frame = 0


Query: 6   RGLFVVIAAAASMLEMGLADQRHMVGGTQGWQQSIDFDSWAEAQTFKVGDELVFNYASGL 65
           R + ++I+  A+ L        H +GG  GW       +WA  QTF VGD LVF+Y +  
Sbjct: 4   REMLIIISVLATTLIGLTVATDHTIGGPSGWTVGASLRTWAAGQTFAVGDNLVFSYPAAF 63

Query: 66  HSMVELPDESAYENCDIGSAIETKSSGKDGIKLTKPGTRYFACGTMGHCSQGMKVKIKIA 125
           H +VE+  +  +++C     + T ++G   + LT PG RYF CG  GHCSQGMK+++ + 
Sbjct: 64  HDVVEV-TKPEFDSCQAVKPLITFANGNSLVPLTTPGKRYFICGMPGHCSQGMKLEVNVV 123

Query: 126 -TGTASSTPSHPSSSSSSSSSSHYSLL 152
            T T + T   P++  S ++ S  S+L
Sbjct: 124 PTATVAPTAPLPNTVPSLNAPSPSSVL 149

[Close]

BLAST of Carg06035 vs. ExPASy Swiss-Prot
Match: P80728 (Mavicyanin OS=Cucurbita pepo OX=3663 PE=1 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 1.5e-17
Identity = 42/106 (39.62%), Postives = 66/106 (62.26%), Query Frame = 0


Query: 28  HMVGGTQGWQQSIDFD--SWAEAQTFKVGDELVFNYASGLHSMVELPDESAYENCDIGSA 87
           H VG + GW   + +D   WA +  F VGD L+FNY +  H+++++ D+  +++C+  S 
Sbjct: 4   HKVGDSTGWTTLVPYDYAKWASSNKFHVGDSLLFNYNNKFHNVLQV-DQEQFKSCNSSSP 63

Query: 88  IETKSSGKDGIKLTKPGTRYFACGTMGHCSQGMKVKIKIATGTASS 132
             + +SG D I L +PGT YF CG  GHC  G KV+IK+  G++S+
Sbjct: 64  AASYTSGADSIPLKRPGTFYFLCGIPGHCQLGQKVEIKVDPGSSSA 108

[Close]

BLAST of Carg06035 vs. ExPASy Swiss-Prot
Match: Q96316 (Uclacyanin-3 OS=Arabidopsis thaliana OX=3702 GN=UCC3 PE=2 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 5.6e-17
Identity = 48/117 (41.03%), Postives = 66/117 (56.41%), Query Frame = 0


Query: 30  VGGTQGWQQSIDFDSWAEAQTFKVGDELVFNYASGLHSMVELPDESAYENCDIGSAIETK 89
           VG   GW  ++D+  W   +TF+VGD L F Y  GL   V + D++ Y+NCD   A +  
Sbjct: 26  VGDISGWTSNLDYTVWLTGKTFRVGDTLEFVY--GLSHSVSVVDKAGYDNCDSSGATQNF 85

Query: 90  SSGKDGIKLTKPGTRYFACGTMGHCSQGMKVKIKI-ATGTASSTPSHPSSSSSSSSS 146
           + G   I LT  GT +F C T GHC  GMK+ + + A   + STPS P S+ S+ SS
Sbjct: 86  ADGDTKIDLTTVGTMHFLCPTFGHCKNGMKLAVPVLAAAPSPSTPSSPPSTPSTPSS 140

[Close]

BLAST of Carg06035 vs. ExPASy Swiss-Prot
Match: O80517 (Uclacyanin-2 OS=Arabidopsis thaliana OX=3702 GN=At2g44790 PE=2 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 5.3e-15
Identity = 52/149 (34.90%), Postives = 77/149 (51.68%), Query Frame = 0


Query: 1   MEGLGRGLFVVIAAAASMLEMGLADQRHMVGGTQGWQQSIDFDSWAEAQTFKVGDELVFN 60
           M GL +    V AA A +L + +      V  T  W   +D+  WA  +TF+VGD L F 
Sbjct: 3   MNGLSK--MAVAAATALLLVLTIVPGAVAVTYTIEWTTGVDYSGWATGKTFRVGDILEFK 62

Query: 61  YASGLHSMVELPDESAYENCDIGSAIETKSSGKDGIKLTKPGTRYFACGTMGHC--SQGM 120
           Y S     V++ D++ Y+ CD  S+ E  S G   I L   G  YF C T GHC  + GM
Sbjct: 63  YGSS--HTVDVVDKAGYDGCDASSSTENHSDGDTKIDLKTVGINYFICSTPGHCRTNGGM 122

Query: 121 KVKIKIATGTAS--STPSHPSSSSSSSSS 146
           K+ + +  G+A   +TP+ PSS+  + ++
Sbjct: 123 KLAVNVVAGSAGPPATPTPPSSTPGTPTT 147

[Close]

BLAST of Carg06035 vs. ExPASy TrEMBL
Match: A0A6J1GWP5 (mavicyanin OS=Cucurbita moschata OX=3662 GN=LOC111457551 PE=4 SV=1)

HSP 1 Score: 308.9 bits (790), Expect = 1.3e-80
Identity = 162/167 (97.01%), Postives = 163/167 (97.60%), Query Frame = 0


Query: 1   MEGLGRGLFVVIAAAASMLEMGLADQRHMVGGTQGWQQSIDFDSWAEAQTFKVGDELVFN 60
           MEGLGRGLFVVIAAAASMLEMGLADQRHMVGGTQGWQQSIDFDSWAEAQTFKVGDELVFN
Sbjct: 1   MEGLGRGLFVVIAAAASMLEMGLADQRHMVGGTQGWQQSIDFDSWAEAQTFKVGDELVFN 60

Query: 61  YASGLHSMVELPDESAYENCDIGSAIETKSSGKDGIKLTKPGTRYFACGTMGHCSQGMKV 120
           YASGLHS+VELPDESAYENCDIGSAIETKSSGKDGIKLTKPGTRYFACGTMGHCSQGMKV
Sbjct: 61  YASGLHSVVELPDESAYENCDIGSAIETKSSGKDGIKLTKPGTRYFACGTMGHCSQGMKV 120

Query: 121 KIKIATGTASSTPSHPSSSSSSSSSSHYSLLGCFLIMASYALLRFML 168
           KIKIATGTASSTPSHP   SSSSSSSHYSLLGCFLIMASYA LRFML
Sbjct: 121 KIKIATGTASSTPSHP---SSSSSSSHYSLLGCFLIMASYA-LRFML 163

[Close]

BLAST of Carg06035 vs. ExPASy TrEMBL
Match: A0A6J1JTY7 (mavicyanin OS=Cucurbita maxima OX=3661 GN=LOC111487816 PE=4 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 5.4e-79
Identity = 157/166 (94.58%), Postives = 162/166 (97.59%), Query Frame = 0


Query: 1   MEGLGRGLFVVIAAAASMLEMGLADQRHMVGGTQGWQQSIDFDSWAEAQTFKVGDELVFN 60
           MEGLGRGLFVVIAAAASMLEMGLADQRH+VGGTQGWQQSIDFDSWAEAQTFKVGD+LVFN
Sbjct: 1   MEGLGRGLFVVIAAAASMLEMGLADQRHVVGGTQGWQQSIDFDSWAEAQTFKVGDQLVFN 60

Query: 61  YASGLHSMVELPDESAYENCDIGSAIETKSSGKDGIKLTKPGTRYFACGTMGHCSQGMKV 120
           YASGLHS+VELPDESAYENCDIGSAIE+KSSGKDGIKLTK GTRYFACGTMGHCSQGMKV
Sbjct: 61  YASGLHSVVELPDESAYENCDIGSAIESKSSGKDGIKLTKAGTRYFACGTMGHCSQGMKV 120

Query: 121 KIKIATGTASSTPSHPSSSSSSSSSSHYSLLGCFLIMASYALLRFM 167
           KIKIATGTASSTPSHPSSS+SSS SSHYSLLGCFLIMA YA LRFM
Sbjct: 121 KIKIATGTASSTPSHPSSSASSSFSSHYSLLGCFLIMAFYA-LRFM 165

[Close]

BLAST of Carg06035 vs. ExPASy TrEMBL
Match: A0A1S3B386 (mavicyanin-like OS=Cucumis melo OX=3656 GN=LOC103485487 PE=4 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 1.3e-61
Identity = 124/162 (76.54%), Postives = 145/162 (89.51%), Query Frame = 0


Query: 6   RGLFV-VIAAAASMLEMGLADQRHMVGGTQGWQQSIDFDSWAEAQTFKVGDELVFNYASG 65
           RG+FV +I  AA M EMGLADQRHMVGG+QGWQ+S+DFDSWA +QTFKVGD++VF Y S 
Sbjct: 11  RGVFVMMIVGAALMAEMGLADQRHMVGGSQGWQESVDFDSWASSQTFKVGDQIVFKYDSS 70

Query: 66  LHSMVELPDESAYENCDIGSAIETKSSGKDGIKLTKPGTRYFACGTMGHCSQGMKVKIKI 125
           LHS+VELP+ES+Y+NCDIG++IE+KSSG D IKLTK GTRYFACGT+GHCSQGMKVKIKI
Sbjct: 71  LHSVVELPEESSYKNCDIGNSIESKSSGNDVIKLTKSGTRYFACGTIGHCSQGMKVKIKI 130

Query: 126 ATGTASSTPSHPSSSSSSSSSSHYSLLGCFLIMASYALLRFM 167
           ATGTASSTPS PSSSSSSSSSSHYSL+G FL++  + +LRFM
Sbjct: 131 ATGTASSTPSSPSSSSSSSSSSHYSLMGFFLMLLPFYVLRFM 172

[Close]

BLAST of Carg06035 vs. ExPASy TrEMBL
Match: A0A5A7UST1 (Mavicyanin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold205G00240 PE=4 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 2.8e-59
Identity = 117/149 (78.52%), Postives = 136/149 (91.28%), Query Frame = 0


Query: 18  MLEMGLADQRHMVGGTQGWQQSIDFDSWAEAQTFKVGDELVFNYASGLHSMVELPDESAY 77
           M EMGLADQRHMVGG+QGWQ+S+DFDSWA +QTFKVGD++VF Y S LHS+VELP+ES+Y
Sbjct: 1   MAEMGLADQRHMVGGSQGWQESVDFDSWASSQTFKVGDQIVFKYDSSLHSVVELPEESSY 60

Query: 78  ENCDIGSAIETKSSGKDGIKLTKPGTRYFACGTMGHCSQGMKVKIKIATGTASSTPSHPS 137
           +NCDIG++IE+KSSG D IKLTK GTRYFACGT+GHCSQGMKVKIKIATGTASSTPS PS
Sbjct: 61  KNCDIGNSIESKSSGNDVIKLTKSGTRYFACGTIGHCSQGMKVKIKIATGTASSTPSSPS 120

Query: 138 SSSSSSSSSHYSLLGCFLIMASYALLRFM 167
           SSSSSSSSSHYSL+G FL++  + +LRFM
Sbjct: 121 SSSSSSSSSHYSLMGFFLMLLPFYVLRFM 149

[Close]

BLAST of Carg06035 vs. ExPASy TrEMBL
Match: A0A6J1C123 (mavicyanin OS=Momordica charantia OX=3673 GN=LOC111006448 PE=4 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 1.2e-57
Identity = 124/163 (76.07%), Postives = 143/163 (87.73%), Query Frame = 0


Query: 1   MEGLGRGLFV-VIAAAASMLEMGLADQRHMVGGTQGWQQSIDFDSWAEAQTFKVGDELVF 60
           MEGL R + V V+ AAASM E   ADQRH+VGG+QGWQ+SIDFDSWA AQTFKVGD+LVF
Sbjct: 1   MEGLWRVVLVMVMVAAASMAEKAWADQRHVVGGSQGWQESIDFDSWASAQTFKVGDQLVF 60

Query: 61  NYASGLHSMVELPDESAYENCDIGSAIETKSSGKDGIKLTKPGTRYFACGTMGHCSQGMK 120
            YASGLHS+VELPDESAY+NCDIGSAIE+KSSG D IKLTKPGTRYFACGT+GHCSQGMK
Sbjct: 61  KYASGLHSVVELPDESAYKNCDIGSAIESKSSGNDAIKLTKPGTRYFACGTIGHCSQGMK 120

Query: 121 VKIKIATGTASSTPSHPSSSSSSSSSSHYSLLGCFLIMASYAL 163
           VKIK+ +GTASSTPS P+SS ++SSS+  SL+G FL++A YAL
Sbjct: 121 VKIKVGSGTASSTPS-PTSSHAASSST--SLVGYFLLLAFYAL 160

[Close]

BLAST of Carg06035 vs. TAIR 10
Match: AT3G27200.1 (Cupredoxin superfamily protein )

HSP 1 Score: 146.7 bits (369), Expect = 1.6e-35
Identity = 70/139 (50.36%), Postives = 102/139 (73.38%), Query Frame = 0


Query: 8   LFVVIAAAASMLEMGLADQRHMVGGTQGWQQSIDFDSWAEAQTFKVGDELVFNYASGLHS 67
           L +++ +    ++  LA  RH++GG+QGW+QS+DFDSW+  Q+FKVGD++VF Y S LHS
Sbjct: 7   LVILVFSGLLSVKTALA-ARHVIGGSQGWEQSVDFDSWSSDQSFKVGDQIVFKY-SELHS 66

Query: 68  MVELPDESAYENCDIGSAIETKSSGKDGIKLTKPGTRYFACGTMGHCSQGMKVKIKIATG 127
           +VEL  E+AY++CD+G+++ + SSG D +KL+K GTRYFACGT+GHC QGMK+K+ + + 
Sbjct: 67  VVELGSETAYKSCDLGTSVNSLSSGNDVVKLSKTGTRYFACGTVGHCEQGMKIKVNVVSS 126

Query: 128 TASSTPSHPSSSSSSSSSS 147
            + S  S   S S S S S
Sbjct: 127 DSKSASSPSGSGSGSDSGS 143

[Close]

BLAST of Carg06035 vs. TAIR 10
Match: AT2G32300.1 (uclacyanin 1 )

HSP 1 Score: 92.8 bits (229), Expect = 2.8e-19
Identity = 52/147 (35.37%), Postives = 81/147 (55.10%), Query Frame = 0


Query: 6   RGLFVVIAAAASMLEMGLADQRHMVGGTQGWQQSIDFDSWAEAQTFKVGDELVFNYASGL 65
           R + ++I+  A+ L        H +GG  GW       +WA  QTF VGD LVF+Y +  
Sbjct: 4   REMLIIISVLATTLIGLTVATDHTIGGPSGWTVGASLRTWAAGQTFAVGDNLVFSYPAAF 63

Query: 66  HSMVELPDESAYENCDIGSAIETKSSGKDGIKLTKPGTRYFACGTMGHCSQGMKVKIKIA 125
           H +VE+  +  +++C     + T ++G   + LT PG RYF CG  GHCSQGMK+++ + 
Sbjct: 64  HDVVEV-TKPEFDSCQAVKPLITFANGNSLVPLTTPGKRYFICGMPGHCSQGMKLEVNVV 123

Query: 126 -TGTASSTPSHPSSSSSSSSSSHYSLL 152
            T T + T   P++  S ++ S  S+L
Sbjct: 124 PTATVAPTAPLPNTVPSLNAPSPSSVL 149

[Close]

BLAST of Carg06035 vs. TAIR 10
Match: AT3G60280.1 (uclacyanin 3 )

HSP 1 Score: 89.0 bits (219), Expect = 4.0e-18
Identity = 48/117 (41.03%), Postives = 66/117 (56.41%), Query Frame = 0


Query: 30  VGGTQGWQQSIDFDSWAEAQTFKVGDELVFNYASGLHSMVELPDESAYENCDIGSAIETK 89
           VG   GW  ++D+  W   +TF+VGD L F Y  GL   V + D++ Y+NCD   A +  
Sbjct: 26  VGDISGWTSNLDYTVWLTGKTFRVGDTLEFVY--GLSHSVSVVDKAGYDNCDSSGATQNF 85

Query: 90  SSGKDGIKLTKPGTRYFACGTMGHCSQGMKVKIKI-ATGTASSTPSHPSSSSSSSSS 146
           + G   I LT  GT +F C T GHC  GMK+ + + A   + STPS P S+ S+ SS
Sbjct: 86  ADGDTKIDLTTVGTMHFLCPTFGHCKNGMKLAVPVLAAAPSPSTPSSPPSTPSTPSS 140

[Close]

BLAST of Carg06035 vs. TAIR 10
Match: AT1G22480.1 (Cupredoxin superfamily protein )

HSP 1 Score: 81.6 bits (200), Expect = 6.4e-16
Identity = 43/109 (39.45%), Postives = 63/109 (57.80%), Query Frame = 0


Query: 33  TQGWQQSIDFDSWAEAQTFKVGDELVFNYASGLHSMVELPDESAYENCDIGSAIETKSSG 92
           T  W    D+      +TF VGD +VFNY +G H++ E+  E+ Y++C +G++I + SSG
Sbjct: 24  TVNWSLGTDYTPLTTGKTFSVGDTIVFNYGAG-HTVDEV-SENDYKSCTLGNSITSDSSG 83

Query: 93  KDGIKLTKPGTRYFACGTMGHCSQGMKVKIKIAT--------GTASSTP 134
              I LT  G RYF CG  GHC+ GMK+ + +A+        GT + TP
Sbjct: 84  TTTIALTTTGPRYFICGIPGHCAAGMKLAVTVASNSSNGVAGGTTTPTP 130

[Close]

BLAST of Carg06035 vs. TAIR 10
Match: AT1G72230.1 (Cupredoxin superfamily protein )

HSP 1 Score: 80.5 bits (197), Expect = 1.4e-15
Identity = 39/97 (40.21%), Postives = 59/97 (60.82%), Query Frame = 0


Query: 36  WQQSIDFDSWAEAQTFKVGDELVFNYASGLHSMVELPDESAYENCDIGSAIETKSSGKDG 95
           W    D+ S A  ++F VGD +VFNY +G H++ E+  ES Y++C +G+AI + SSG   
Sbjct: 31  WSLGKDYSSLATGKSFAVGDTIVFNYGAG-HTVDEV-SESDYKSCTLGNAISSDSSGTTS 90

Query: 96  IKLTKPGTRYFACGTMGHCSQGMKVKIKIATGTASST 133
           I L  PG  YF CG  GHC+ GMK+ + +   ++  +
Sbjct: 91  IALKTPGPHYFICGIPGHCTGGMKLSVIVPAASSGGS 125

The following BLAST results are available for this feature:

BLAST of Carg06035 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7033356.1	4.6e-85	100.00	Uclacyanin 1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6602670.1	7.4e-83	98.80	Uclacyanin 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022955584.1	2.6e-80	97.01	mavicyanin [Cucurbita moschata]	[more]
XP_022991129.1	1.1e-78	94.58	mavicyanin [Cucurbita maxima]	[more]
XP_008441346.1	2.7e-61	76.54	PREDICTED: mavicyanin-like [Cucumis melo]	[more]

BLAST of Carg06035 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q41001	9.3e-20	43.80	Blue copper protein OS=Pisum sativum OX=3888 PE=2 SV=1	[more]
O82081	3.9e-18	35.37	Uclacyanin 1 OS=Arabidopsis thaliana OX=3702 GN=UCC1 PE=1 SV=1	[more]
P80728	1.5e-17	39.62	Mavicyanin OS=Cucurbita pepo OX=3663 PE=1 SV=1	[more]
Q96316	5.6e-17	41.03	Uclacyanin-3 OS=Arabidopsis thaliana OX=3702 GN=UCC3 PE=2 SV=1	[more]
O80517	5.3e-15	34.90	Uclacyanin-2 OS=Arabidopsis thaliana OX=3702 GN=At2g44790 PE=2 SV=1	[more]

BLAST of Carg06035 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GWP5	1.3e-80	97.01	mavicyanin OS=Cucurbita moschata OX=3662 GN=LOC111457551 PE=4 SV=1	[more]
A0A6J1JTY7	5.4e-79	94.58	mavicyanin OS=Cucurbita maxima OX=3661 GN=LOC111487816 PE=4 SV=1	[more]
A0A1S3B386	1.3e-61	76.54	mavicyanin-like OS=Cucumis melo OX=3656 GN=LOC103485487 PE=4 SV=1	[more]
A0A5A7UST1	2.8e-59	78.52	Mavicyanin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold205G0024...	[more]
A0A6J1C123	1.2e-57	76.07	mavicyanin OS=Momordica charantia OX=3673 GN=LOC111006448 PE=4 SV=1	[more]

BLAST of Carg06035 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G27200.1	1.6e-35	50.36	Cupredoxin superfamily protein	[more]
AT2G32300.1	2.8e-19	35.37	uclacyanin 1	[more]
AT3G60280.1	4.0e-18	41.03	uclacyanin 3	[more]
AT1G22480.1	6.4e-16	39.45	Cupredoxin superfamily protein	[more]
AT1G72230.1	1.4e-15	40.21	Cupredoxin superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003245	Phytocyanin domain	PFAM	PF02298	Cu_bind_like	coord: 36..118 e-value: 7.2E-24 score: 83.6
IPR003245	Phytocyanin domain	PROSITE	PS51485	PHYTOCYANIN	coord: 26..126 score: 32.867786
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 25..131 e-value: 9.6E-33 score: 114.6
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 27..124
IPR039391	Phytocyanin	PANTHER	PTHR33021	BLUE COPPER PROTEIN	coord: 10..140
None	No IPR available	PANTHER	PTHR33021:SF396	BNAA06G32330D PROTEIN	coord: 10..140
None	No IPR available	CDD	cd04216	Phytocyanin	coord: 28..124 e-value: 4.83907E-45 score: 141.629

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg06035-RA	Carg06035-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0022900	electron transport chain
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0046658	anchored component of plasma membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0009055	electron transfer activity