Carg06016 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg06016
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	thylakoid lumenal 15 kDa protein 1, chloroplastic
Location	Carg_Chr04: 21548006 .. 21550417 (-)
RNA-Seq Expression	Carg06016
Synteny	Carg06016

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGAAAAGAAAAGAAAAGAAATCTGGGAAGAACTGCAGCTTTGGAAATGGCTCTGCTCAACGCCTCATTATGTTCTTCCACTTGCAGAATCCCTTCCAGAACTCACCTTCCTCTTCCTCCCTCTATTTCCGCCTTTTCCAATCCTCAGGTAAACTTCACCTCTCAATTTCTTCTCTGCTTCTCTCATTTTGTACTGAAATGCTATGATTGCGACCAAAAAAATGAAGGCGATGAAGCACCAAGTGGAAGCTACTCGGAATTTTGTTCTTTCCGTTGGAGAATCCGCCTCTAAAGCCAGCTTCCTCGCTTTGATTTCCGCCTCCTTGTTCCTCGCTGATCCTGCGCTAGCTTTTAAGGTTAAATTGTTAAGCGTATATATCTTTCTTGTTCTGATTGTTTCGAGTGAATGTCTGTTGTTATATCTCTGTATTTGAAGCCGCTTCAATCTGATCATTCACTGATTTTGGGTGAACGGATTCGATTTCGTAATTAGGGCGGAGGTCCTTACGGTGCTGGGGTCACTAGAGGTCAGGATCTCACTGGGAAAGATTTCAGTGGGAAGACCTTGATCAAGCAAGATTTCAAAACGGTTTCTCCCTTTTCAAACAAATTCAATTTTTCCTCATGTTGTGTTTCTGAAATAGCTTTACGTTTCTTTTCTCACCAGTCTATATTACGGCAAGCTAATTTTAAAGGTGCAAAGTTATTAGGTGCTAGCTTCTTCGATGCTGATTTAACAGGTTCGTTGTTTCAGTGTTCGTAAGTAATTTTTATGAACTGCCAACTCTTCCTTGGAGAACAAATTCCCATTAAAGCTGAGTTCAATTGTTTCTTTCTTTGCCTAATTGTTAGGAGCGGATCTTTCTGATGCTGATCTCCGAGGAGCAGACTTTTCTTTGGCCAATGTTACCAAGGTGATGTATGTTTATTCCTGTTAACAGTATCATCTTTAGTTGATTTCAATCTCATTCTATGCACAAAGTAGGTTATGTGATGCATTGAAATCTGCAGTTTCTATCTGAGATTTTCCATTCAAGTTTTGGTTCATGTTGTGCTTTGATCTTAGAAGCCAATGAAATTATAGTCTTTATCCTCGATGGTTTCTTCTACTTTCGGCAGGCAAATTTAAGAAATGCCAACTTAGAGGGAGCGCTTGCTACAGGAAACACTTCTTTTCGTGGATCAATAATAAATGGAGCTGGTATGTATGTAAAGCTAACATTTATGTAGCTTTTAGTATTTCAAGGGCGATCCTCTCTCCAAAATTGTCTTAGTTAATAAAAGACATTAATATAGGTTGTTACTCCAACCCCTCTATTTTTATCAAATTTGAAATTCTAAACCTATAGCTCTAGTGTAACAACCCAAGTCTATTGGAAATCGATATTGTCCGCTTTGGTCGGTTACGTATTGTCATTTGTTTCACGATTCTAAAATGTGTCTAGGAGAGGTTTCCACACCCTTATAAGCAATGCTTCATTTCCCTTTCTAACCAATGTAGGATCTCACAATCCCAGACGTGCCTTTGCGAGATCCCAGATACGATTTTAAAACGCGTCTACTAAGAAGACACCATGCTATAAAGGGGGTGGATTGTGAGATCCTACATTGATTGGAGAGGGGAAAGAAGCATTCATTATAAGGGTTTGGAAACCTCTCCTTAGTAGACGCGTTTTCAAACTGTGAGACTAACGATAATATGTAACGGGCCAAAGTGAACAACATTTGCTAGCGATGAGCTTGAACCGTTACATTTAGTTACTCGTGTTAGGGGTGCAAACAAACTGTCACATTCGTTAGAAAAAGGAGTAATCTTAGGTAGATAAGGAAGGACAAATATCTCTATGAGGAAATACTGATGGTTAATTCATCTCATGATGTCAGACTTCACAGACGTGCCTTTGCGAGAGGATCAGCGGGAGTACCTCTGCAAAGTTGCAGATGGGTAAACAATACTTTCCTCTGTTCTCAAAATGGTTCAATAAAGATTAAAGAAAACATTTTAGATCCTTGAACTTTGATGAAAGTAACAATTTAGTCACTTTAACTTCAAATGTTACATGAAAGTAACATTTTGGATCCTTAAACTTCCATGAAAGTGGTTTTTACTGGTAAATAAACTAATGAACTCAGTCCTTTCTGTTGACAGGGTGAATCCAATGACTGGAAATGCAACCCGGGAGACCTTGCTCTGCAATTGAGAGTCGAATAATATGGCGAGCTACTAAGGTACAAAGAATCATAAAGATTTACCTTAAAATGTAATGAATTTAATAACTATGTGAGTATCTAATCCAATGAGTTCAGTTTTGTCATTCTTCTGTTAATATCTCCTCATACCCTGTTTTCGCGGCTTGTCCATATCATAAAGCTTATGAAAAGTAACACATTCGAAGAATCAGAACACATTGGA

mRNA sequence

AGAAAAGAAAAGAAAAGAAATCTGGGAAGAACTGCAGCTTTGGAAATGGCTCTGCTCAACGCCTCATTATGTTCTTCCACTTGCAGAATCCCTTCCAGAACTCACCTTCCTCTTCCTCCCTCTATTTCCGCCTTTTCCAATCCTCAGGCGATGAAGCACCAAGTGGAAGCTACTCGGAATTTTGTTCTTTCCGTTGGAGAATCCGCCTCTAAAGCCAGCTTCCTCGCTTTGATTTCCGCCTCCTTGTTCCTCGCTGATCCTGCGCTAGCTTTTAAGGGCGGAGGTCCTTACGGTGCTGGGGTCACTAGAGGTCAGGATCTCACTGGGAAAGATTTCAGTGGGAAGACCTTGATCAAGCAAGATTTCAAAACGTCTATATTACGGCAAGCTAATTTTAAAGGTGCAAAGTTATTAGGTGCTAGCTTCTTCGATGCTGATTTAACAGGAGCGGATCTTTCTGATGCTGATCTCCGAGGAGCAGACTTTTCTTTGGCCAATGTTACCAAGGCAAATTTAAGAAATGCCAACTTAGAGGGAGCGCTTGCTACAGGAAACACTTCTTTTCGTGGATCAATAATAAATGGAGCTGACTTCACAGACGTGCCTTTGCGAGAGGATCAGCGGGAGTACCTCTGCAAAGTTGCAGATGGGGTGAATCCAATGACTGGAAATGCAACCCGGGAGACCTTGCTCTGCAATTGAGAGTCGAATAATATGGCGAGCTACTAAGGTACAAAGAATCATAAAGATTTACCTTAAAATGTAATGAATTTAATAACTATGTGAGTATCTAATCCAATGAGTTCAGTTTTGTCATTCTTCTGTTAATATCTCCTCATACCCTGTTTTCGCGGCTTGTCCATATCATAAAGCTTATGAAAAGTAACACATTCGAAGAATCAGAACACATTGGA

Coding sequence (CDS)

ATGGCTCTGCTCAACGCCTCATTATGTTCTTCCACTTGCAGAATCCCTTCCAGAACTCACCTTCCTCTTCCTCCCTCTATTTCCGCCTTTTCCAATCCTCAGGCGATGAAGCACCAAGTGGAAGCTACTCGGAATTTTGTTCTTTCCGTTGGAGAATCCGCCTCTAAAGCCAGCTTCCTCGCTTTGATTTCCGCCTCCTTGTTCCTCGCTGATCCTGCGCTAGCTTTTAAGGGCGGAGGTCCTTACGGTGCTGGGGTCACTAGAGGTCAGGATCTCACTGGGAAAGATTTCAGTGGGAAGACCTTGATCAAGCAAGATTTCAAAACGTCTATATTACGGCAAGCTAATTTTAAAGGTGCAAAGTTATTAGGTGCTAGCTTCTTCGATGCTGATTTAACAGGAGCGGATCTTTCTGATGCTGATCTCCGAGGAGCAGACTTTTCTTTGGCCAATGTTACCAAGGCAAATTTAAGAAATGCCAACTTAGAGGGAGCGCTTGCTACAGGAAACACTTCTTTTCGTGGATCAATAATAAATGGAGCTGACTTCACAGACGTGCCTTTGCGAGAGGATCAGCGGGAGTACCTCTGCAAAGTTGCAGATGGGGTGAATCCAATGACTGGAAATGCAACCCGGGAGACCTTGCTCTGCAATTGA

Protein sequence

MALLNASLCSSTCRIPSRTHLPLPPSISAFSNPQAMKHQVEATRNFVLSVGESASKASFLALISASLFLADPALAFKGGGPYGAGVTRGQDLTGKDFSGKTLIKQDFKTSILRQANFKGAKLLGASFFDADLTGADLSDADLRGADFSLANVTKANLRNANLEGALATGNTSFRGSIINGADFTDVPLREDQREYLCKVADGVNPMTGNATRETLLCN

Homology

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BLAST of Carg06016 vs. NCBI nr
Match: KAG7033337.1 (Thylakoid lumenal 15 kDa protein 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 421.8 bits (1083), Expect = 3.6e-114
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 0


Query: 1   MALLNASLCSSTCRIPSRTHLPLPPSISAFSNPQAMKHQVEATRNFVLSVGESASKASFL 60
           MALLNASLCSSTCRIPSRTHLPLPPSISAFSNPQAMKHQVEATRNFVLSVGESASKASFL
Sbjct: 1   MALLNASLCSSTCRIPSRTHLPLPPSISAFSNPQAMKHQVEATRNFVLSVGESASKASFL 60

Query: 61  ALISASLFLADPALAFKGGGPYGAGVTRGQDLTGKDFSGKTLIKQDFKTSILRQANFKGA 120
           ALISASLFLADPALAFKGGGPYGAGVTRGQDLTGKDFSGKTLIKQDFKTSILRQANFKGA
Sbjct: 61  ALISASLFLADPALAFKGGGPYGAGVTRGQDLTGKDFSGKTLIKQDFKTSILRQANFKGA 120

Query: 121 KLLGASFFDADLTGADLSDADLRGADFSLANVTKANLRNANLEGALATGNTSFRGSIING 180
           KLLGASFFDADLTGADLSDADLRGADFSLANVTKANLRNANLEGALATGNTSFRGSIING
Sbjct: 121 KLLGASFFDADLTGADLSDADLRGADFSLANVTKANLRNANLEGALATGNTSFRGSIING 180

Query: 181 ADFTDVPLREDQREYLCKVADGVNPMTGNATRETLLCN 219
           ADFTDVPLREDQREYLCKVADGVNPMTGNATRETLLCN
Sbjct: 181 ADFTDVPLREDQREYLCKVADGVNPMTGNATRETLLCN 218

[Close]

BLAST of Carg06016 vs. NCBI nr
Match: KAG6602651.1 (Thylakoid lumenal 15 kDa protein 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 415.2 bits (1066), Expect = 3.4e-112
Identity = 215/218 (98.62%), Postives = 215/218 (98.62%), Query Frame = 0


Query: 1   MALLNASLCSSTCRIPSRTHLPLPPSISAFSNPQAMKHQVEATRNFVLSVGESASKASFL 60
           MALLNASLCSSTCRIPSR HLPLPPSI AFSNPQAMKHQVEATRNFVLSVGESASKASFL
Sbjct: 1   MALLNASLCSSTCRIPSRPHLPLPPSIPAFSNPQAMKHQVEATRNFVLSVGESASKASFL 60

Query: 61  ALISASLFLADPALAFKGGGPYGAGVTRGQDLTGKDFSGKTLIKQDFKTSILRQANFKGA 120
           ALISASLFLADPALAFKGGGPYGAGVTRGQDLTGKDFSGKTLIKQDFKTSILRQANFKGA
Sbjct: 61  ALISASLFLADPALAFKGGGPYGAGVTRGQDLTGKDFSGKTLIKQDFKTSILRQANFKGA 120

Query: 121 KLLGASFFDADLTGADLSDADLRGADFSLANVTKANLRNANLEGALATGNTSFRGSIING 180
           KLLGASFFDADLTGADLSDADLRGADFSLANVTKANL NANLEGALATGNTSFRGSIING
Sbjct: 121 KLLGASFFDADLTGADLSDADLRGADFSLANVTKANLSNANLEGALATGNTSFRGSIING 180

Query: 181 ADFTDVPLREDQREYLCKVADGVNPMTGNATRETLLCN 219
           ADFTDVPLREDQREYLCKVADGVNPMTGNATRETLLCN
Sbjct: 181 ADFTDVPLREDQREYLCKVADGVNPMTGNATRETLLCN 218

[Close]

BLAST of Carg06016 vs. NCBI nr
Match: XP_022955551.1 (thylakoid lumenal 15 kDa protein 1, chloroplastic isoform X1 [Cucurbita moschata])

HSP 1 Score: 412.9 bits (1060), Expect = 1.7e-111
Identity = 213/218 (97.71%), Postives = 214/218 (98.17%), Query Frame = 0


Query: 1   MALLNASLCSSTCRIPSRTHLPLPPSISAFSNPQAMKHQVEATRNFVLSVGESASKASFL 60
           MALLNASLCSSTCRIPSR HLPLPPS+SAFSNPQ MKHQVEATRNFVLSVGESASKASFL
Sbjct: 1   MALLNASLCSSTCRIPSRPHLPLPPSVSAFSNPQTMKHQVEATRNFVLSVGESASKASFL 60

Query: 61  ALISASLFLADPALAFKGGGPYGAGVTRGQDLTGKDFSGKTLIKQDFKTSILRQANFKGA 120
           ALISASLFLADPALAFKGGGPYGAGVTRGQDLTGKDFSGKTLIKQDFKTSILRQANFKGA
Sbjct: 61  ALISASLFLADPALAFKGGGPYGAGVTRGQDLTGKDFSGKTLIKQDFKTSILRQANFKGA 120

Query: 121 KLLGASFFDADLTGADLSDADLRGADFSLANVTKANLRNANLEGALATGNTSFRGSIING 180
           KLLGASFFDADLTGADLSDADLRGADFSLANVTKANL NANLEGALATGNTSFRGSIING
Sbjct: 121 KLLGASFFDADLTGADLSDADLRGADFSLANVTKANLSNANLEGALATGNTSFRGSIING 180

Query: 181 ADFTDVPLREDQREYLCKVADGVNPMTGNATRETLLCN 219
           ADFTDVPLREDQREYLCKVADGVNP TGNATRETLLCN
Sbjct: 181 ADFTDVPLREDQREYLCKVADGVNPTTGNATRETLLCN 218

[Close]

BLAST of Carg06016 vs. NCBI nr
Match: XP_022955559.1 (thylakoid lumenal 15 kDa protein 1, chloroplastic isoform X2 [Cucurbita moschata])

HSP 1 Score: 412.9 bits (1060), Expect = 1.7e-111
Identity = 213/218 (97.71%), Postives = 214/218 (98.17%), Query Frame = 0


Query: 1   MALLNASLCSSTCRIPSRTHLPLPPSISAFSNPQAMKHQVEATRNFVLSVGESASKASFL 60
           MALLNASLCSSTCRIPSR HLPLPPS+SAFSNPQ MKHQVEATRNFVLSVGESASKASFL
Sbjct: 1   MALLNASLCSSTCRIPSRPHLPLPPSVSAFSNPQVMKHQVEATRNFVLSVGESASKASFL 60

Query: 61  ALISASLFLADPALAFKGGGPYGAGVTRGQDLTGKDFSGKTLIKQDFKTSILRQANFKGA 120
           ALISASLFLADPALAFKGGGPYGAGVTRGQDLTGKDFSGKTLIKQDFKTSILRQANFKGA
Sbjct: 61  ALISASLFLADPALAFKGGGPYGAGVTRGQDLTGKDFSGKTLIKQDFKTSILRQANFKGA 120

Query: 121 KLLGASFFDADLTGADLSDADLRGADFSLANVTKANLRNANLEGALATGNTSFRGSIING 180
           KLLGASFFDADLTGADLSDADLRGADFSLANVTKANL NANLEGALATGNTSFRGSIING
Sbjct: 121 KLLGASFFDADLTGADLSDADLRGADFSLANVTKANLSNANLEGALATGNTSFRGSIING 180

Query: 181 ADFTDVPLREDQREYLCKVADGVNPMTGNATRETLLCN 219
           ADFTDVPLREDQREYLCKVADGVNP TGNATRETLLCN
Sbjct: 181 ADFTDVPLREDQREYLCKVADGVNPTTGNATRETLLCN 218

[Close]

BLAST of Carg06016 vs. NCBI nr
Match: XP_022991096.1 (thylakoid lumenal 15 kDa protein 1, chloroplastic isoform X1 [Cucurbita maxima])

HSP 1 Score: 412.1 bits (1058), Expect = 2.9e-111
Identity = 213/218 (97.71%), Postives = 215/218 (98.62%), Query Frame = 0


Query: 1   MALLNASLCSSTCRIPSRTHLPLPPSISAFSNPQAMKHQVEATRNFVLSVGESASKASFL 60
           MALLNASLCSSTCRIPSR+HLPLPPSI AFSNP+AMKHQVEATRNFVLSVGESASKASFL
Sbjct: 1   MALLNASLCSSTCRIPSRSHLPLPPSIPAFSNPRAMKHQVEATRNFVLSVGESASKASFL 60

Query: 61  ALISASLFLADPALAFKGGGPYGAGVTRGQDLTGKDFSGKTLIKQDFKTSILRQANFKGA 120
           ALISASLFLADPALAFKGGGPYGAGVTRGQDLTGKDFSGKTLIKQDFKTSILRQANFKGA
Sbjct: 61  ALISASLFLADPALAFKGGGPYGAGVTRGQDLTGKDFSGKTLIKQDFKTSILRQANFKGA 120

Query: 121 KLLGASFFDADLTGADLSDADLRGADFSLANVTKANLRNANLEGALATGNTSFRGSIING 180
           KLLGASFFDADLTGADLSDADLRGADFSLANVTKANL NANLEGALATGNTSFRGSIING
Sbjct: 121 KLLGASFFDADLTGADLSDADLRGADFSLANVTKANLSNANLEGALATGNTSFRGSIING 180

Query: 181 ADFTDVPLREDQREYLCKVADGVNPMTGNATRETLLCN 219
           ADFTDVPLREDQREYLCKVADGVNP TGNATRETLLCN
Sbjct: 181 ADFTDVPLREDQREYLCKVADGVNPTTGNATRETLLCN 218

[Close]

BLAST of Carg06016 vs. ExPASy Swiss-Prot
Match: O22160 (Thylakoid lumenal 15 kDa protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g44920 PE=1 SV=2)

HSP 1 Score: 287.7 bits (735), Expect = 1.1e-76
Identity = 159/224 (70.98%), Postives = 174/224 (77.68%), Query Frame = 0


Query: 1   MALLNASLCSSTCRIPSRTHLPLPPSISAF-----SNPQAMKHQVEA-TRNFVLSVGESA 60
           M +L+     S C I  +  L  P S S+      S  Q  K  V +  R+ V S+GE  
Sbjct: 1   MVILSNVSLFSCCNISQKPSLFSPSSRSSHCPIRCSQSQEGKEVVTSPLRSVVWSLGEEV 60

Query: 61  SKASFLALISASLFLADPALAFKGGGPYGAGVTRGQDLTGKDFSGKTLIKQDFKTSILRQ 120
           SK S  AL+SASLF  DPALAFKGGGPYG GVTRGQDL+GKDFSG+TLI+QDFKTSILRQ
Sbjct: 61  SKRSLFALVSASLFFVDPALAFKGGGPYGQGVTRGQDLSGKDFSGQTLIRQDFKTSILRQ 120

Query: 121 ANFKGAKLLGASFFDADLTGADLSDADLRGADFSLANVTKANLRNANLEGALATGNTSFR 180
           ANFKGAKLLGASFFDADLTGADLS+ADLRGADFSLANVTK NL NANLEGA  TGNTSF+
Sbjct: 121 ANFKGAKLLGASFFDADLTGADLSEADLRGADFSLANVTKVNLTNANLEGATVTGNTSFK 180

Query: 181 GSIINGADFTDVPLREDQREYLCKVADGVNPMTGNATRETLLCN 219
           GS I GADFTDVPLR+DQR YLCKVADGVN  TGNATR+TLLCN
Sbjct: 181 GSNITGADFTDVPLRDDQRVYLCKVADGVNATTGNATRDTLLCN 224

[Close]

BLAST of Carg06016 vs. ExPASy Swiss-Prot
Match: B1WVN5 (Pentapeptide repeat protein Rfr32 OS=Crocosphaera subtropica (strain ATCC 51142 / BH68) OX=43989 GN=rfr32 PE=1 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 2.6e-14
Identity = 55/126 (43.65%), Postives = 69/126 (54.76%), Query Frame = 0


Query: 92  LTGKDFSGKTLIKQDFKTSILRQANFKGAKLLGASFFDADLTGADLSDADLRGADFSLAN 151
           L G+DFSGK+L    F  + L  +NF  A L GA F  + L GADL  ADL      L +
Sbjct: 54  LIGEDFSGKSLTYAQFTNADLTDSNFSEADLRGAVFNGSALIGADLHGADLTNGLAYLTS 113

Query: 152 VTKANLRNANLEGALATGNTSFRGSIINGADFTDVPLREDQREYLCKVADGVNPMTGNAT 211
              A+L NA L  A+    T F  + I GADF+   L   + + LC  ADGVNP TG +T
Sbjct: 114 FKGADLTNAVLTEAIMM-RTKFDDAKITGADFSLAVLDVYEVDKLCDRADGVNPKTGVST 173

Query: 212 RETLLC 218
           RE+L C
Sbjct: 174 RESLGC 178

[Close]

BLAST of Carg06016 vs. ExPASy Swiss-Prot
Match: Q8H1Q1 (Thylakoid lumenal protein TL20.3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TL20.3 PE=1 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 7.4e-09
Identity = 52/164 (31.71%), Postives = 80/164 (48.78%), Query Frame = 0


Query: 54  ASKASFLALISASLFLADPALAFKGGGPYGAGVTRGQDLTGKDFSGKTLIKQDFKTSILR 113
           AS +   A+   + F AD    F  G     G     DL+    S +   + +F ++ +R
Sbjct: 81  ASSSGVPAMAELNRFEADTRGEFGIGSAAQYG---SADLSKTVHSNENFRRANFTSADMR 140

Query: 114 QANFKGAKLLGASFFDADLTGADLSDADLRGADFSLANVTKANLRNANLEGALATGNTSF 173
           +++F G+   GA    A    A+ S ADL         + +ANL NA L  ++ T  +  
Sbjct: 141 ESDFSGSTFNGAYLEKAVAYKANFSGADLSDTLMDRMVLNEANLTNAVLVRSVLT-RSDL 200

Query: 174 RGSIINGADFTDVPLREDQREYLCKVADGVNPMTGNATRETLLC 218
            G+ I GADF+D  +   Q++ LCK A G NP+TG  TR++L C
Sbjct: 201 GGAKIEGADFSDAVIDLLQKQALCKYATGTNPLTGVDTRKSLGC 240

[Close]

BLAST of Carg06016 vs. ExPASy Swiss-Prot
Match: Q9SE95 (FH protein interacting protein FIP2 OS=Arabidopsis thaliana OX=3702 GN=FIP2 PE=1 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 3.1e-07
Identity = 42/111 (37.84%), Postives = 53/111 (47.75%), Query Frame = 0


Query: 88  RGQDLTGKDFSGKTLIKQDFKTSILRQANFKGAKLLGASFFDADLTGADLSDADLRGADF 147
           R  D  G  F    L + +F ++ LR A   G  L  A+  DA L G     ADLR A  
Sbjct: 174 RNADAEGSIFHNAILRECEFTSANLRGALLAGTNLQSANLQDACLVGCSFCGADLRTAHL 233

Query: 148 SLANVTKANLRNANLEGALATG----NTSFRGSIINGADFTDVPLREDQRE 195
             A++T ANL  ANLEGA   G    N +F+G+ +  A    V LRE   E
Sbjct: 234 QNADLTNANLEGANLEGANLKGAKLSNANFKGANLQRAYLRHVNLREAHME 284

[Close]

BLAST of Carg06016 vs. ExPASy Swiss-Prot
Match: P73709 (Uncharacterized protein slr1819 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=slr1819 PE=4 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 4.0e-07
Identity = 40/97 (41.24%), Postives = 53/97 (54.64%), Query Frame = 0


Query: 91  DLTGKDFSGKTLIKQDFKTSILRQANFKGAKLLGASFFDADLTGADLSD-----ADLRGA 150
           +L   + SG +L + D   + LR ANF GA L GA   D+D+T A L D     ADLRGA
Sbjct: 61  NLVAANLSGASLNQADLAGADLRSANFHGAMLQGAILRDSDMTLATLQDTNLIGADLRGA 120

Query: 151 DFSLANVTKANLRNANLEGALATGNTSFRGSIINGAD 183
           D S A +T A LR AN+        T+ + +I+  AD
Sbjct: 121 DLSGATLTGACLRGANMRQEKKGYYTNLQAAILGRAD 157

[Close]

BLAST of Carg06016 vs. ExPASy TrEMBL
Match: A0A6J1GWM1 (thylakoid lumenal 15 kDa protein 1, chloroplastic isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111457530 PE=4 SV=1)

HSP 1 Score: 412.9 bits (1060), Expect = 8.2e-112
Identity = 213/218 (97.71%), Postives = 214/218 (98.17%), Query Frame = 0


Query: 1   MALLNASLCSSTCRIPSRTHLPLPPSISAFSNPQAMKHQVEATRNFVLSVGESASKASFL 60
           MALLNASLCSSTCRIPSR HLPLPPS+SAFSNPQ MKHQVEATRNFVLSVGESASKASFL
Sbjct: 1   MALLNASLCSSTCRIPSRPHLPLPPSVSAFSNPQVMKHQVEATRNFVLSVGESASKASFL 60

Query: 61  ALISASLFLADPALAFKGGGPYGAGVTRGQDLTGKDFSGKTLIKQDFKTSILRQANFKGA 120
           ALISASLFLADPALAFKGGGPYGAGVTRGQDLTGKDFSGKTLIKQDFKTSILRQANFKGA
Sbjct: 61  ALISASLFLADPALAFKGGGPYGAGVTRGQDLTGKDFSGKTLIKQDFKTSILRQANFKGA 120

Query: 121 KLLGASFFDADLTGADLSDADLRGADFSLANVTKANLRNANLEGALATGNTSFRGSIING 180
           KLLGASFFDADLTGADLSDADLRGADFSLANVTKANL NANLEGALATGNTSFRGSIING
Sbjct: 121 KLLGASFFDADLTGADLSDADLRGADFSLANVTKANLSNANLEGALATGNTSFRGSIING 180

Query: 181 ADFTDVPLREDQREYLCKVADGVNPMTGNATRETLLCN 219
           ADFTDVPLREDQREYLCKVADGVNP TGNATRETLLCN
Sbjct: 181 ADFTDVPLREDQREYLCKVADGVNPTTGNATRETLLCN 218

[Close]

BLAST of Carg06016 vs. ExPASy TrEMBL
Match: A0A6J1GU31 (thylakoid lumenal 15 kDa protein 1, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111457530 PE=4 SV=1)

HSP 1 Score: 412.9 bits (1060), Expect = 8.2e-112
Identity = 213/218 (97.71%), Postives = 214/218 (98.17%), Query Frame = 0


Query: 1   MALLNASLCSSTCRIPSRTHLPLPPSISAFSNPQAMKHQVEATRNFVLSVGESASKASFL 60
           MALLNASLCSSTCRIPSR HLPLPPS+SAFSNPQ MKHQVEATRNFVLSVGESASKASFL
Sbjct: 1   MALLNASLCSSTCRIPSRPHLPLPPSVSAFSNPQTMKHQVEATRNFVLSVGESASKASFL 60

Query: 61  ALISASLFLADPALAFKGGGPYGAGVTRGQDLTGKDFSGKTLIKQDFKTSILRQANFKGA 120
           ALISASLFLADPALAFKGGGPYGAGVTRGQDLTGKDFSGKTLIKQDFKTSILRQANFKGA
Sbjct: 61  ALISASLFLADPALAFKGGGPYGAGVTRGQDLTGKDFSGKTLIKQDFKTSILRQANFKGA 120

Query: 121 KLLGASFFDADLTGADLSDADLRGADFSLANVTKANLRNANLEGALATGNTSFRGSIING 180
           KLLGASFFDADLTGADLSDADLRGADFSLANVTKANL NANLEGALATGNTSFRGSIING
Sbjct: 121 KLLGASFFDADLTGADLSDADLRGADFSLANVTKANLSNANLEGALATGNTSFRGSIING 180

Query: 181 ADFTDVPLREDQREYLCKVADGVNPMTGNATRETLLCN 219
           ADFTDVPLREDQREYLCKVADGVNP TGNATRETLLCN
Sbjct: 181 ADFTDVPLREDQREYLCKVADGVNPTTGNATRETLLCN 218

[Close]

BLAST of Carg06016 vs. ExPASy TrEMBL
Match: A0A6J1JPS6 (thylakoid lumenal 15 kDa protein 1, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111487791 PE=4 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 1.4e-111
Identity = 213/218 (97.71%), Postives = 215/218 (98.62%), Query Frame = 0


Query: 1   MALLNASLCSSTCRIPSRTHLPLPPSISAFSNPQAMKHQVEATRNFVLSVGESASKASFL 60
           MALLNASLCSSTCRIPSR+HLPLPPSI AFSNP+AMKHQVEATRNFVLSVGESASKASFL
Sbjct: 1   MALLNASLCSSTCRIPSRSHLPLPPSIPAFSNPRAMKHQVEATRNFVLSVGESASKASFL 60

Query: 61  ALISASLFLADPALAFKGGGPYGAGVTRGQDLTGKDFSGKTLIKQDFKTSILRQANFKGA 120
           ALISASLFLADPALAFKGGGPYGAGVTRGQDLTGKDFSGKTLIKQDFKTSILRQANFKGA
Sbjct: 61  ALISASLFLADPALAFKGGGPYGAGVTRGQDLTGKDFSGKTLIKQDFKTSILRQANFKGA 120

Query: 121 KLLGASFFDADLTGADLSDADLRGADFSLANVTKANLRNANLEGALATGNTSFRGSIING 180
           KLLGASFFDADLTGADLSDADLRGADFSLANVTKANL NANLEGALATGNTSFRGSIING
Sbjct: 121 KLLGASFFDADLTGADLSDADLRGADFSLANVTKANLSNANLEGALATGNTSFRGSIING 180

Query: 181 ADFTDVPLREDQREYLCKVADGVNPMTGNATRETLLCN 219
           ADFTDVPLREDQREYLCKVADGVNP TGNATRETLLCN
Sbjct: 181 ADFTDVPLREDQREYLCKVADGVNPTTGNATRETLLCN 218

[Close]

BLAST of Carg06016 vs. ExPASy TrEMBL
Match: A0A6J1JKT4 (thylakoid lumenal 15 kDa protein 1, chloroplastic isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111487791 PE=4 SV=1)

HSP 1 Score: 410.6 bits (1054), Expect = 4.1e-111
Identity = 212/218 (97.25%), Postives = 214/218 (98.17%), Query Frame = 0


Query: 1   MALLNASLCSSTCRIPSRTHLPLPPSISAFSNPQAMKHQVEATRNFVLSVGESASKASFL 60
           MALLNASLCSSTCRIPSR+HLPLPPSI AFSNP+ MKHQVEATRNFVLSVGESASKASFL
Sbjct: 1   MALLNASLCSSTCRIPSRSHLPLPPSIPAFSNPRVMKHQVEATRNFVLSVGESASKASFL 60

Query: 61  ALISASLFLADPALAFKGGGPYGAGVTRGQDLTGKDFSGKTLIKQDFKTSILRQANFKGA 120
           ALISASLFLADPALAFKGGGPYGAGVTRGQDLTGKDFSGKTLIKQDFKTSILRQANFKGA
Sbjct: 61  ALISASLFLADPALAFKGGGPYGAGVTRGQDLTGKDFSGKTLIKQDFKTSILRQANFKGA 120

Query: 121 KLLGASFFDADLTGADLSDADLRGADFSLANVTKANLRNANLEGALATGNTSFRGSIING 180
           KLLGASFFDADLTGADLSDADLRGADFSLANVTKANL NANLEGALATGNTSFRGSIING
Sbjct: 121 KLLGASFFDADLTGADLSDADLRGADFSLANVTKANLSNANLEGALATGNTSFRGSIING 180

Query: 181 ADFTDVPLREDQREYLCKVADGVNPMTGNATRETLLCN 219
           ADFTDVPLREDQREYLCKVADGVNP TGNATRETLLCN
Sbjct: 181 ADFTDVPLREDQREYLCKVADGVNPTTGNATRETLLCN 218

[Close]

BLAST of Carg06016 vs. ExPASy TrEMBL
Match: A0A6J1BVJ8 (thylakoid lumenal 15 kDa protein 1, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111006104 PE=4 SV=1)

HSP 1 Score: 372.1 bits (954), Expect = 1.6e-99
Identity = 193/218 (88.53%), Postives = 201/218 (92.20%), Query Frame = 0


Query: 1   MALLNASLCSSTCRIPSRTHLPLPPSISAFSNPQAMKHQVEATRNFVLSVGESASKASFL 60
           MALLNASLC ST RIP   HL LPPSISA   P  +K Q+EA+RN +LSVGESASKA+FL
Sbjct: 1   MALLNASLCCSTSRIPPAPHLHLPPSISAHPTPPVLKQQLEASRNLILSVGESASKATFL 60

Query: 61  ALISASLFLADPALAFKGGGPYGAGVTRGQDLTGKDFSGKTLIKQDFKTSILRQANFKGA 120
           AL+SASLFLADPALAFKGGGPYGAGVTRGQDL+GKDFSGKTLIKQDFKTSILRQANFKGA
Sbjct: 61  ALLSASLFLADPALAFKGGGPYGAGVTRGQDLSGKDFSGKTLIKQDFKTSILRQANFKGA 120

Query: 121 KLLGASFFDADLTGADLSDADLRGADFSLANVTKANLRNANLEGALATGNTSFRGSIING 180
           KLLGASFFDADLTGADLSDADLRGADFSLANVTKANL NANLEGALATGNTSFRGS I G
Sbjct: 121 KLLGASFFDADLTGADLSDADLRGADFSLANVTKANLSNANLEGALATGNTSFRGSTIEG 180

Query: 181 ADFTDVPLREDQREYLCKVADGVNPMTGNATRETLLCN 219
           ADFTDVPLREDQREYLCKVADGVNP TGNATR+TLLCN
Sbjct: 181 ADFTDVPLREDQREYLCKVADGVNPTTGNATRDTLLCN 218

[Close]

BLAST of Carg06016 vs. TAIR 10
Match: AT2G44920.2 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 287.7 bits (735), Expect = 7.7e-78
Identity = 159/224 (70.98%), Postives = 174/224 (77.68%), Query Frame = 0


Query: 1   MALLNASLCSSTCRIPSRTHLPLPPSISAF-----SNPQAMKHQVEA-TRNFVLSVGESA 60
           M +L+     S C I  +  L  P S S+      S  Q  K  V +  R+ V S+GE  
Sbjct: 1   MVILSNVSLFSCCNISQKPSLFSPSSRSSHCPIRCSQSQEGKEVVTSPLRSVVWSLGEEV 60

Query: 61  SKASFLALISASLFLADPALAFKGGGPYGAGVTRGQDLTGKDFSGKTLIKQDFKTSILRQ 120
           SK S  AL+SASLF  DPALAFKGGGPYG GVTRGQDL+GKDFSG+TLI+QDFKTSILRQ
Sbjct: 61  SKRSLFALVSASLFFVDPALAFKGGGPYGQGVTRGQDLSGKDFSGQTLIRQDFKTSILRQ 120

Query: 121 ANFKGAKLLGASFFDADLTGADLSDADLRGADFSLANVTKANLRNANLEGALATGNTSFR 180
           ANFKGAKLLGASFFDADLTGADLS+ADLRGADFSLANVTK NL NANLEGA  TGNTSF+
Sbjct: 121 ANFKGAKLLGASFFDADLTGADLSEADLRGADFSLANVTKVNLTNANLEGATVTGNTSFK 180

Query: 181 GSIINGADFTDVPLREDQREYLCKVADGVNPMTGNATRETLLCN 219
           GS I GADFTDVPLR+DQR YLCKVADGVN  TGNATR+TLLCN
Sbjct: 181 GSNITGADFTDVPLRDDQRVYLCKVADGVNATTGNATRDTLLCN 224

[Close]

BLAST of Carg06016 vs. TAIR 10
Match: AT2G44920.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 219.5 bits (558), Expect = 2.6e-57
Identity = 127/187 (67.91%), Postives = 140/187 (74.87%), Query Frame = 0


Query: 1   MALLNASLCSSTCRIPSRTHLPLPPSISAF-----SNPQAMKHQVEA-TRNFVLSVGESA 60
           M +L+     S C I  +  L  P S S+      S  Q  K  V +  R+ V S+GE  
Sbjct: 1   MVILSNVSLFSCCNISQKPSLFSPSSRSSHCPIRCSQSQEGKEVVTSPLRSVVWSLGEEV 60

Query: 61  SKASFLALISASLFLADPALAFKGGGPYGAGVTRGQDLTGKDFSGKTLIKQDFKTSILRQ 120
           SK S  AL+SASLF  DPALAFKGGGPYG GVTRGQDL+GKDFSG+TLI+QDFKTSILRQ
Sbjct: 61  SKRSLFALVSASLFFVDPALAFKGGGPYGQGVTRGQDLSGKDFSGQTLIRQDFKTSILRQ 120

Query: 121 ANFKGAKLLGASFFDADLTGADLSDADLRGADFSLANVTKANLRNANLEGALATGNTSFR 180
           ANFKGAKLLGASFFDADLTGADLS+ADLRGADFSLANVTK NL NANLEGA  TGNTSF+
Sbjct: 121 ANFKGAKLLGASFFDADLTGADLSEADLRGADFSLANVTKVNLTNANLEGATVTGNTSFK 180

Query: 181 GSIINGA 182
           GS I GA
Sbjct: 181 GSNITGA 187

[Close]

BLAST of Carg06016 vs. TAIR 10
Match: AT1G12250.1 (Pentapeptide repeat-containing protein )

HSP 1 Score: 62.4 bits (150), Expect = 5.2e-10
Identity = 52/164 (31.71%), Postives = 80/164 (48.78%), Query Frame = 0


Query: 54  ASKASFLALISASLFLADPALAFKGGGPYGAGVTRGQDLTGKDFSGKTLIKQDFKTSILR 113
           AS +   A+   + F AD    F  G     G     DL+    S +   + +F ++ +R
Sbjct: 81  ASSSGVPAMAELNRFEADTRGEFGIGSAAQYG---SADLSKTVHSNENFRRANFTSADMR 140

Query: 114 QANFKGAKLLGASFFDADLTGADLSDADLRGADFSLANVTKANLRNANLEGALATGNTSF 173
           +++F G+   GA    A    A+ S ADL         + +ANL NA L  ++ T  +  
Sbjct: 141 ESDFSGSTFNGAYLEKAVAYKANFSGADLSDTLMDRMVLNEANLTNAVLVRSVLT-RSDL 200

Query: 174 RGSIINGADFTDVPLREDQREYLCKVADGVNPMTGNATRETLLC 218
            G+ I GADF+D  +   Q++ LCK A G NP+TG  TR++L C
Sbjct: 201 GGAKIEGADFSDAVIDLLQKQALCKYATGTNPLTGVDTRKSLGC 240

[Close]

BLAST of Carg06016 vs. TAIR 10
Match: AT1G12250.2 (Pentapeptide repeat-containing protein )

HSP 1 Score: 62.4 bits (150), Expect = 5.2e-10
Identity = 52/164 (31.71%), Postives = 80/164 (48.78%), Query Frame = 0


Query: 54  ASKASFLALISASLFLADPALAFKGGGPYGAGVTRGQDLTGKDFSGKTLIKQDFKTSILR 113
           AS +   A+   + F AD    F  G     G     DL+    S +   + +F ++ +R
Sbjct: 7   ASSSGVPAMAELNRFEADTRGEFGIGSAAQYG---SADLSKTVHSNENFRRANFTSADMR 66

Query: 114 QANFKGAKLLGASFFDADLTGADLSDADLRGADFSLANVTKANLRNANLEGALATGNTSF 173
           +++F G+   GA    A    A+ S ADL         + +ANL NA L  ++ T  +  
Sbjct: 67  ESDFSGSTFNGAYLEKAVAYKANFSGADLSDTLMDRMVLNEANLTNAVLVRSVLT-RSDL 126

Query: 174 RGSIINGADFTDVPLREDQREYLCKVADGVNPMTGNATRETLLC 218
            G+ I GADF+D  +   Q++ LCK A G NP+TG  TR++L C
Sbjct: 127 GGAKIEGADFSDAVIDLLQKQALCKYATGTNPLTGVDTRKSLGC 166

[Close]

BLAST of Carg06016 vs. TAIR 10
Match: AT5G55000.2 (potassium channel tetramerisation domain-containing protein / pentapeptide repeat-containing protein )

HSP 1 Score: 57.0 bits (136), Expect = 2.2e-08
Identity = 42/111 (37.84%), Postives = 53/111 (47.75%), Query Frame = 0


Query: 88  RGQDLTGKDFSGKTLIKQDFKTSILRQANFKGAKLLGASFFDADLTGADLSDADLRGADF 147
           R  D  G  F    L + +F ++ LR A   G  L  A+  DA L G     ADLR A  
Sbjct: 174 RNADAEGSIFHNAILRECEFTSANLRGALLAGTNLQSANLQDACLVGCSFCGADLRTAHL 233

Query: 148 SLANVTKANLRNANLEGALATG----NTSFRGSIINGADFTDVPLREDQRE 195
             A++T ANL  ANLEGA   G    N +F+G+ +  A    V LRE   E
Sbjct: 234 QNADLTNANLEGANLEGANLKGAKLSNANFKGANLQRAYLRHVNLREAHME 284

The following BLAST results are available for this feature:

BLAST of Carg06016 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7033337.1	3.6e-114	100.00	Thylakoid lumenal 15 kDa protein 1, chloroplastic [Cucurbita argyrosperma subsp....	[more]
KAG6602651.1	3.4e-112	98.62	Thylakoid lumenal 15 kDa protein 1, chloroplastic, partial [Cucurbita argyrosper...	[more]
XP_022955551.1	1.7e-111	97.71	thylakoid lumenal 15 kDa protein 1, chloroplastic isoform X1 [Cucurbita moschata...	[more]
XP_022955559.1	1.7e-111	97.71	thylakoid lumenal 15 kDa protein 1, chloroplastic isoform X2 [Cucurbita moschata...	[more]
XP_022991096.1	2.9e-111	97.71	thylakoid lumenal 15 kDa protein 1, chloroplastic isoform X1 [Cucurbita maxima]	[more]

BLAST of Carg06016 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22160	1.1e-76	70.98	Thylakoid lumenal 15 kDa protein 1, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]
B1WVN5	2.6e-14	43.65	Pentapeptide repeat protein Rfr32 OS=Crocosphaera subtropica (strain ATCC 51142 ...	[more]
Q8H1Q1	7.4e-09	31.71	Thylakoid lumenal protein TL20.3, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]
Q9SE95	3.1e-07	37.84	FH protein interacting protein FIP2 OS=Arabidopsis thaliana OX=3702 GN=FIP2 PE=1...	[more]
P73709	4.0e-07	41.24	Uncharacterized protein slr1819 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) ...	[more]

BLAST of Carg06016 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GWM1	8.2e-112	97.71	thylakoid lumenal 15 kDa protein 1, chloroplastic isoform X2 OS=Cucurbita moscha...	[more]
A0A6J1GU31	8.2e-112	97.71	thylakoid lumenal 15 kDa protein 1, chloroplastic isoform X1 OS=Cucurbita moscha...	[more]
A0A6J1JPS6	1.4e-111	97.71	thylakoid lumenal 15 kDa protein 1, chloroplastic isoform X1 OS=Cucurbita maxima...	[more]
A0A6J1JKT4	4.1e-111	97.25	thylakoid lumenal 15 kDa protein 1, chloroplastic isoform X2 OS=Cucurbita maxima...	[more]
A0A6J1BVJ8	1.6e-99	88.53	thylakoid lumenal 15 kDa protein 1, chloroplastic isoform X1 OS=Momordica charan...	[more]

BLAST of Carg06016 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G44920.2	7.7e-78	70.98	Tetratricopeptide repeat (TPR)-like superfamily protein	[more]
AT2G44920.1	2.6e-57	67.91	Tetratricopeptide repeat (TPR)-like superfamily protein	[more]
AT1G12250.1	5.2e-10	31.71	Pentapeptide repeat-containing protein	[more]
AT1G12250.2	5.2e-10	31.71	Pentapeptide repeat-containing protein	[more]
AT5G55000.2	2.2e-08	37.84	potassium channel tetramerisation domain-containing protein / pentapeptide repea...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001646	Pentapeptide repeat	PFAM	PF00805	Pentapeptide	coord: 110..148 e-value: 1.2E-12 score: 47.2
None	No IPR available	GENE3D	2.160.20.80		coord: 75..218 e-value: 1.2E-46 score: 159.4
None	No IPR available	SUPERFAMILY	141571	Pentapeptide repeat-like	coord: 87..189
IPR044213	Thylakoid lumenal 15kDa protein 1-like	PANTHER	PTHR47200	THYLAKOID LUMENAL 15 KDA PROTEIN 1, CHLOROPLASTIC	coord: 27..218

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg06016-RA	Carg06016-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0009534	chloroplast thylakoid