Carg05931 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg05931 vs. NCBI nr
Match: KAG7033254.1 (Cytokinin hydroxylase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1050.8 bits (2716), Expect = 3.8e-303
Identity = 521/521 (100.00%), Postives = 521/521 (100.00%), Query Frame = 0

Query: 1   MAAFILPLVITTLALAATSFVILSVLYDAVSRYWWTPRRIKKKMAMQGVGGPKPRPFLGN 60
           MAAFILPLVITTLALAATSFVILSVLYDAVSRYWWTPRRIKKKMAMQGVGGPKPRPFLGN
Sbjct: 1   MAAFILPLVITTLALAATSFVILSVLYDAVSRYWWTPRRIKKKMAMQGVGGPKPRPFLGN 60

Query: 61  ILHVTALLSNSTSQDMPSISHDLVPRLLPHYLVWTTQFGKRFIFWNGIEPRMCLAETDLI 120
           ILHVTALLSNSTSQDMPSISHDLVPRLLPHYLVWTTQFGKRFIFWNGIEPRMCLAETDLI
Sbjct: 61  ILHVTALLSNSTSQDMPSISHDLVPRLLPHYLVWTTQFGKRFIFWNGIEPRMCLAETDLI 120

Query: 121 KELLSKHSAVTGKSWLQQQGSNHFIGRGLLMANGQNWFHQRHMVAPAFLGNRLKSYAGYM 180
           KELLSKHSAVTGKSWLQQQGSNHFIGRGLLMANGQNWFHQRHMVAPAFLGNRLKSYAGYM
Sbjct: 121 KELLSKHSAVTGKSWLQQQGSNHFIGRGLLMANGQNWFHQRHMVAPAFLGNRLKSYAGYM 180

Query: 181 VECTREMLISLENEVKSGRTEFEIGECMTRITADIISKTEFGSSYEKGKQIFHLLTTLQN 240
           VECTREMLISLENEVKSGRTEFEIGECMTRITADIISKTEFGSSYEKGKQIFHLLTTLQN
Sbjct: 181 VECTREMLISLENEVKSGRTEFEIGECMTRITADIISKTEFGSSYEKGKQIFHLLTTLQN 240

Query: 241 LCAQASRHLCLPGSRFFPSKYNREIKALKGKMEGLLMEIIESRKECVENGNDLLGMLLNE 300
           LCAQASRHLCLPGSRFFPSKYNREIKALKGKMEGLLMEIIESRKECVENGNDLLGMLLNE
Sbjct: 241 LCAQASRHLCLPGSRFFPSKYNREIKALKGKMEGLLMEIIESRKECVENGNDLLGMLLNE 300

Query: 301 MQNKRDNKGFNLNLQIIMDECKTFFFAGHETTALLLTWTLMLLATNPSWQHKVRAEVMAV 360
           MQNKRDNKGFNLNLQIIMDECKTFFFAGHETTALLLTWTLMLLATNPSWQHKVRAEVMAV
Sbjct: 301 MQNKRDNKGFNLNLQIIMDECKTFFFAGHETTALLLTWTLMLLATNPSWQHKVRAEVMAV 360

Query: 361 CNRQAPSLDHLPKLSLLGKVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVL 420
           CNRQAPSLDHLPKLSLLGKVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVL
Sbjct: 361 CNRQAPSLDHLPKLSLLGKVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVL 420

Query: 421 AIHHSEELWGKDANEFNPERFESSKAFTSGRFIPFASGPRNCVGQSFAVMETKIILAMLI 480
           AIHHSEELWGKDANEFNPERFESSKAFTSGRFIPFASGPRNCVGQSFAVMETKIILAMLI
Sbjct: 421 AIHHSEELWGKDANEFNPERFESSKAFTSGRFIPFASGPRNCVGQSFAVMETKIILAMLI 480

Query: 481 AKFSFTISPNYRHAPVVVLTIKPKYGVQVCLTPVTSSLVGL 522
           AKFSFTISPNYRHAPVVVLTIKPKYGVQVCLTPVTSSLVGL
Sbjct: 481 AKFSFTISPNYRHAPVVVLTIKPKYGVQVCLTPVTSSLVGL 521

BLAST of Carg05931 vs. NCBI nr
Match: XP_022954721.1 (cytokinin hydroxylase-like [Cucurbita moschata])

HSP 1 Score: 1045.4 bits (2702), Expect = 1.6e-301
Identity = 518/521 (99.42%), Postives = 519/521 (99.62%), Query Frame = 0

Query: 1   MAAFILPLVITTLALAATSFVILSVLYDAVSRYWWTPRRIKKKMAMQGVGGPKPRPFLGN 60
           MAAFILPLVITTLALAATSFVILSVLYDAVS YWWTPRRIKKKMAMQGVGGPKPRPFLGN
Sbjct: 1   MAAFILPLVITTLALAATSFVILSVLYDAVSCYWWTPRRIKKKMAMQGVGGPKPRPFLGN 60

Query: 61  ILHVTALLSNSTSQDMPSISHDLVPRLLPHYLVWTTQFGKRFIFWNGIEPRMCLAETDLI 120
           ILHVTALLSNSTSQDMPS+SHDLVPRLLPHYLVWT QFGKRFIFWNGIEPRMCLAETDLI
Sbjct: 61  ILHVTALLSNSTSQDMPSVSHDLVPRLLPHYLVWTAQFGKRFIFWNGIEPRMCLAETDLI 120

Query: 121 KELLSKHSAVTGKSWLQQQGSNHFIGRGLLMANGQNWFHQRHMVAPAFLGNRLKSYAGYM 180
           KELLSKHSAVTGKSWLQQQGSNHFIGRGLLMANGQNWFHQRHMVAPAFLGNRLKSYAGYM
Sbjct: 121 KELLSKHSAVTGKSWLQQQGSNHFIGRGLLMANGQNWFHQRHMVAPAFLGNRLKSYAGYM 180

Query: 181 VECTREMLISLENEVKSGRTEFEIGECMTRITADIISKTEFGSSYEKGKQIFHLLTTLQN 240
           VECTREMLISLENEVKSGRTEFEIGECMTRITADIISKTEFGSSYEKGKQIFHLLTTLQN
Sbjct: 181 VECTREMLISLENEVKSGRTEFEIGECMTRITADIISKTEFGSSYEKGKQIFHLLTTLQN 240

Query: 241 LCAQASRHLCLPGSRFFPSKYNREIKALKGKMEGLLMEIIESRKECVENGNDLLGMLLNE 300
           LCAQASRHLCLPGSRFFPSKYNREIKALKGKMEGLLMEIIESRKECVENGNDLLGMLLNE
Sbjct: 241 LCAQASRHLCLPGSRFFPSKYNREIKALKGKMEGLLMEIIESRKECVENGNDLLGMLLNE 300

Query: 301 MQNKRDNKGFNLNLQIIMDECKTFFFAGHETTALLLTWTLMLLATNPSWQHKVRAEVMAV 360
           MQNKRDNKGFNLNLQIIMDECKTFFFAGHETTALLLTWTLMLLATNPSWQHKVRAEVMAV
Sbjct: 301 MQNKRDNKGFNLNLQIIMDECKTFFFAGHETTALLLTWTLMLLATNPSWQHKVRAEVMAV 360

Query: 361 CNRQAPSLDHLPKLSLLGKVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVL 420
           CNRQAPSLDHLPKLSLLGKVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVL
Sbjct: 361 CNRQAPSLDHLPKLSLLGKVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVL 420

Query: 421 AIHHSEELWGKDANEFNPERFESSKAFTSGRFIPFASGPRNCVGQSFAVMETKIILAMLI 480
           AIHHSEELWGKDANEFNPERFESSKAFTSGRFIPFASGPRNCVGQSFAVMETKIILAMLI
Sbjct: 421 AIHHSEELWGKDANEFNPERFESSKAFTSGRFIPFASGPRNCVGQSFAVMETKIILAMLI 480

Query: 481 AKFSFTISPNYRHAPVVVLTIKPKYGVQVCLTPVTSSLVGL 522
           AKFSFTISPNYRHAPVVVLTIKPKYGVQVCLTPVTSSLVGL
Sbjct: 481 AKFSFTISPNYRHAPVVVLTIKPKYGVQVCLTPVTSSLVGL 521

BLAST of Carg05931 vs. NCBI nr
Match: XP_023532069.1 (cytokinin hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 983.8 bits (2542), Expect = 5.7e-283
Identity = 489/514 (95.14%), Postives = 496/514 (96.50%), Query Frame = 0

Query: 1   MAAFILPLVITTLALAATSFVILSVLYDAVSRYWWTPRRIKKKMAMQGVGGPKPRPFLGN 60
           MAAFILPLVI TLALAATSFVILSVLYDAVS YWWTPRRIK+KMAMQGVGGPKPRPFLGN
Sbjct: 1   MAAFILPLVIATLALAATSFVILSVLYDAVSCYWWTPRRIKEKMAMQGVGGPKPRPFLGN 60

Query: 61  ILHVTALLSNSTSQDMPSISHDLVPRLLPHYLVWTTQFGKRFIFWNGIEPRMCLAETDLI 120
           IL VTALLS STSQDMPSISHDLVPRLLPHYLVWT QFG  F  WNGIEPRMCLAETDLI
Sbjct: 61  ILDVTALLSKSTSQDMPSISHDLVPRLLPHYLVWTAQFGMSFFXWNGIEPRMCLAETDLI 120

Query: 121 KELLSKHSAVTGKSWLQQQGSNHFIGRGLLMANGQNWFHQRHMVAPAFLGNRLKSYAGYM 180
           KELLSKHSAVTGKSWLQQQGSNHFIGRGLLMANGQNWFHQRH+VAPAFLGNRLKSY GYM
Sbjct: 121 KELLSKHSAVTGKSWLQQQGSNHFIGRGLLMANGQNWFHQRHVVAPAFLGNRLKSYVGYM 180

Query: 181 VECTREMLISLENEVKSGRTEFEIGECMTRITADIISKTEFGSSYEKGKQIFHLLTTLQN 240
           VECTREMLISLENEVK GRTE EIGE MTRITADIISKTEFGSSYEKGKQIFHLLTTLQN
Sbjct: 181 VECTREMLISLENEVKLGRTELEIGEYMTRITADIISKTEFGSSYEKGKQIFHLLTTLQN 240

Query: 241 LCAQASRHLCLPGSRFFPSKYNREIKALKGKMEGLLMEIIESRKECVENGNDLLGMLLNE 300
           LCAQASRHLCLPGSRFFPSKYNREIKALKGK+EGLLMEIIESRKECVENGNDLLGMLLNE
Sbjct: 241 LCAQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIESRKECVENGNDLLGMLLNE 300

Query: 301 MQNKRDNKGFNLNLQIIMDECKTFFFAGHETTALLLTWTLMLLATNPSWQHKVRAEVMAV 360
           MQNKRDNKGFNLNLQIIMDECKTFFFAGHETTALLLTWT+MLLATNPSWQHKVRAEV+AV
Sbjct: 301 MQNKRDNKGFNLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQHKVRAEVIAV 360

Query: 361 CNRQAPSLDHLPKLSLLGKVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVL 420
           CNRQAPSLDHLPKLSLLG VINESLRLYPPASILPRMAFEDIKL DLEIPKGL+IWIPVL
Sbjct: 361 CNRQAPSLDHLPKLSLLGMVINESLRLYPPASILPRMAFEDIKLRDLEIPKGLAIWIPVL 420

Query: 421 AIHHSEELWGKDANEFNPERFESSKAFTSGRFIPFASGPRNCVGQSFAVMETKIILAMLI 480
           AIHHSEELWGKDANEFNPERF SSKAFTSGRFIPFA GPRNCVGQSFAVMETKIILAMLI
Sbjct: 421 AIHHSEELWGKDANEFNPERFGSSKAFTSGRFIPFAFGPRNCVGQSFAVMETKIILAMLI 480

Query: 481 AKFSFTISPNYRHAPVVVLTIKPKYGVQVCLTPV 515
           AKFSFTIS NYRHAPVVVLTIKPKYGVQVCLTP+
Sbjct: 481 AKFSFTISQNYRHAPVVVLTIKPKYGVQVCLTPL 514

BLAST of Carg05931 vs. NCBI nr
Match: XP_022990121.1 (cytokinin hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 983.0 bits (2540), Expect = 9.8e-283
Identity = 492/514 (95.72%), Postives = 497/514 (96.69%), Query Frame = 0

Query: 1   MAAFILPLVITTLALAATSFVILSVLYDAVSRYWWTPRRIKKKMAMQGVGGPKPRPFLGN 60
           MAAFILPLVITTLALAATSFVILSVLYDAVS YW TPRRIKKKMAMQGVGGPKPRPFLGN
Sbjct: 1   MAAFILPLVITTLALAATSFVILSVLYDAVSCYWSTPRRIKKKMAMQGVGGPKPRPFLGN 60

Query: 61  ILHVTALLSNSTSQDMPSISHDLVPRLLPHYLVWTTQFGKRFIFWNGIEPRMCLAETDLI 120
           IL VTALLS STSQDMPSISHDLVPRLLPHYLVWT QFGKRFIFWNGIEPRMCLAETDLI
Sbjct: 61  ILDVTALLSKSTSQDMPSISHDLVPRLLPHYLVWTAQFGKRFIFWNGIEPRMCLAETDLI 120

Query: 121 KELLSKHSAVTGKSWLQQQGSNHFIGRGLLMANGQNWFHQRHMVAPAFLGNRLKSYAGYM 180
           KELLSKHSAVTGKSWLQQQGS+HFIGRGLLMANGQNWFHQRH+VAPAFLGNRLKSYAG M
Sbjct: 121 KELLSKHSAVTGKSWLQQQGSSHFIGRGLLMANGQNWFHQRHVVAPAFLGNRLKSYAGCM 180

Query: 181 VECTREMLISLENEVKSGRTEFEIGECMTRITADIISKTEFGSSYEKGKQIFHLLTTLQN 240
           VECTREMLISLENEVK GRTEFEIGE MTRITADIISKTEFGSSYEKGKQIFHLLTTLQN
Sbjct: 181 VECTREMLISLENEVKLGRTEFEIGEYMTRITADIISKTEFGSSYEKGKQIFHLLTTLQN 240

Query: 241 LCAQASRHLCLPGSRFFPSKYNREIKALKGKMEGLLMEIIESRKECVENGNDLLGMLLNE 300
           LCAQASRHLCLPGSRFFPSKYNREIKALKGK+EGLLMEIIESRKECVENGNDLLGMLLNE
Sbjct: 241 LCAQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIESRKECVENGNDLLGMLLNE 300

Query: 301 MQNKRDNKGFNLNLQIIMDECKTFFFAGHETTALLLTWTLMLLATNPSWQHKVRAEVMAV 360
           MQNKRDNKGFNLNLQIIMDECKTFFFAGHETTALLLTWTLMLLATNPSWQ KVRAEVMAV
Sbjct: 301 MQNKRDNKGFNLNLQIIMDECKTFFFAGHETTALLLTWTLMLLATNPSWQDKVRAEVMAV 360

Query: 361 CNRQAPSLDHLPKLSLLGKVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVL 420
           CNRQAPSLDHLPKLS L  VINESLRLYPPASILPR+AFEDIKLGDLEIPKGLSIWIPVL
Sbjct: 361 CNRQAPSLDHLPKLSQLSMVINESLRLYPPASILPRIAFEDIKLGDLEIPKGLSIWIPVL 420

Query: 421 AIHHSEELWGKDANEFNPERFESSKAFTSGRFIPFASGPRNCVGQSFAVMETKIILAMLI 480
           AIHHSEELWGKDANEF PERF SSK FTSGRFIPFASGPRNCVGQSFAVMETKIILAMLI
Sbjct: 421 AIHHSEELWGKDANEFKPERFGSSKVFTSGRFIPFASGPRNCVGQSFAVMETKIILAMLI 480

Query: 481 AKFSFTISPNYRHAPVVVLTIKPKYGVQVCLTPV 515
           AKFSF IS NYRHAPVVVLTIKPKYGVQVCLTP+
Sbjct: 481 AKFSFAISQNYRHAPVVVLTIKPKYGVQVCLTPL 514

BLAST of Carg05931 vs. NCBI nr
Match: XP_038890905.1 (cytokinin hydroxylase-like [Benincasa hispida])

HSP 1 Score: 882.1 bits (2278), Expect = 2.4e-252
Identity = 435/518 (83.98%), Postives = 470/518 (90.73%), Query Frame = 0

Query: 5   ILPLVITTLALAATSFVILSVLYDAVSRYWWTPRRIKKKMAMQGVGGPKPRPFLGNILHV 64
           +LPL++TTL L  TSF I S+LYD +S Y +TPRRIK KMA QGV GPKPRP LGNIL V
Sbjct: 4   MLPLLLTTLVLLTTSFFIFSLLYDTLSCYCFTPRRIKHKMAKQGVRGPKPRPLLGNILDV 63

Query: 65  TALLSNSTSQDMPSISHDLVPRLLPHYLVWTTQFGKRFIFWNGIEPRMCLAETDLIKELL 124
            ALLS STS DMPSISHDLVPRLLPH+L W+TQFGKRFIFWNGIEPRMCL ETDLIKELL
Sbjct: 64  AALLSKSTSSDMPSISHDLVPRLLPHFLAWSTQFGKRFIFWNGIEPRMCLTETDLIKELL 123

Query: 125 SKHSAVTGKSWLQQQGSNHFIGRGLLMANGQNWFHQRHMVAPAFLGNRLKSYAGYMVECT 184
           SK+SAV+GKSWLQQQGS HFIGRGLLMANGQNWFHQRH+VAPAF+G+RLKSYAGYMVECT
Sbjct: 124 SKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVECT 183

Query: 185 REMLISLENEVKSGRTEFEIGECMTRITADIISKTEFGSSYEKGKQIFHLLTTLQNLCAQ 244
           REML S+ENEVKSGR+EFEIGE MTR+TADIIS+TEF SSYEKGKQIFHLLT LQ+LCAQ
Sbjct: 184 REMLESIENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQ 243

Query: 245 ASRHLCLPGSRFFPSKYNREIKALKGKMEGLLMEIIESRKECVE------NGNDLLGMLL 304
           ASRHLCLPGSRFFPSKYNREIKALKGK+E LLMEII+SR++CVE       GNDLLGMLL
Sbjct: 244 ASRHLCLPGSRFFPSKYNREIKALKGKVEELLMEIIQSRRDCVEIGRSSSYGNDLLGMLL 303

Query: 305 NEMQNKRDNKGFNLNLQIIMDECKTFFFAGHETTALLLTWTLMLLATNPSWQHKVRAEVM 364
           NEMQ K+D  G +LNLQIIMDECKTFFFAGHETTALLLTWT+MLLATNPSWQHK+RAEV+
Sbjct: 304 NEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQHKLRAEVI 363

Query: 365 AVCNRQAPSLDHLPKLSLLGKVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIP 424
           A+C  + PS  HL KLSLLG VINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIP
Sbjct: 364 AICGSETPSFHHLSKLSLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIP 423

Query: 425 VLAIHHSEELWGKDANEFNPERFESSKAFTSGRFIPFASGPRNCVGQSFAVMETKIILAM 484
           VLAIHHSEE+WGKDANEFNPERF +SKAFTSG FIPFASGPRNCVGQSFA+METKIILAM
Sbjct: 424 VLAIHHSEEIWGKDANEFNPERFANSKAFTSGGFIPFASGPRNCVGQSFALMETKIILAM 483

Query: 485 LIAKFSFTISPNYRHAPVVVLTIKPKYGVQVCLTPVTS 517
           LI+KFSFTIS  YRHAPVVVLTIKPKYGVQVCL P+ +
Sbjct: 484 LISKFSFTISDKYRHAPVVVLTIKPKYGVQVCLKPLNT 521

BLAST of Carg05931 vs. ExPASy Swiss-Prot
Match: Q9FF18 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1)

HSP 1 Score: 708.8 bits (1828), Expect = 4.7e-203
Identity = 352/519 (67.82%), Postives = 416/519 (80.15%), Query Frame = 0

Query: 8   LVITTLALAATSFV--ILSVLYDAVSRYWWTPRRIKKKMAMQGVGGPKPRPFLGNILHVT 67
           +++T L      FV  IL VLYD +S YW TPRRIKK M  QGV GPKPRP  GNIL ++
Sbjct: 1   MLLTILKSLLVIFVTTILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEIS 60

Query: 68  ALLSNSTSQDMPSISHDLVPRLLPHYLVWTTQFGKRFIFWNGIEPRMCLAETDLIKELLS 127
           A++S S S+D  SI HD+V RLLPHY+ W+ Q+GKRFI WNG +PR+CL ET+LIKELL 
Sbjct: 61  AMVSQSASKDCDSIHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELIKELLM 120

Query: 128 KHSAVTGKSWLQQQGSNHFIGRGLLMANGQNWFHQRHMVAPAFLGNRLKSYAGYMVECTR 187
           KH+ V+G+SWLQQQG+ +FIGRGLLMANGQ+W HQRH+ APAF G RLK YA +MVECT 
Sbjct: 121 KHNGVSGRSWLQQQGTKNFIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHMVECTS 180

Query: 188 EMLISLENEVKSGRTEFEIGECMTRITADIISKTEFGSSYEKGKQIFHLLTTLQNLCAQA 247
           +++  L  EV  G  E EIGE M ++TADIIS+T+FGSS+EKGK++F+ LT LQ  CAQA
Sbjct: 181 KLVERLRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRRCAQA 240

Query: 248 SRHLCLPGSRFFPSKYNREIKALKGKMEGLLMEIIESRKECVE------NGNDLLGMLLN 307
           +RHLC PGSRF PSKYNREIK+LK ++E LL+EII+SR++C E      +G+DLLG+LLN
Sbjct: 241 TRHLCFPGSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDDLLGLLLN 300

Query: 308 EMQNKRDNKGFNLNLQIIMDECKTFFFAGHETTALLLTWTLMLLATNPSWQHKVRAEVMA 367
           EM   ++N   N NLQ+IMDECKTFFFAGHETTALLLTWT MLLA NP+WQ KVR EV  
Sbjct: 301 EMDIDKNNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEKVREEVRE 360

Query: 368 VCNRQA-PSLDHLPKLSLLGKVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIP 427
           V  R   PS+D L KL+ L KVINESLRLYPPA++LPRMAFED+KLGDL IPKGLSIWIP
Sbjct: 361 VFGRNGLPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPKGLSIWIP 420

Query: 428 VLAIHHSEELWGKDANEFNPERFESSKAFTSGR-FIPFASGPRNCVGQSFAVMETKIILA 487
           VLAIHHSEELWGKDAN+FNPERF   + F SGR FIPFA+GPRNC+GQ FA+ME KIILA
Sbjct: 421 VLAIHHSEELWGKDANQFNPERF-GGRPFASGRHFIPFAAGPRNCIGQQFALMEAKIILA 480

Query: 488 MLIAKFSFTISPNYRHAPVVVLTIKPKYGVQVCLTPVTS 517
            LI+KF+FTIS NYRHAP+VVLTIKPKYGVQV L P+ S
Sbjct: 481 TLISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPLVS 518

BLAST of Carg05931 vs. ExPASy Swiss-Prot
Match: Q9ZW95 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1)

HSP 1 Score: 678.3 bits (1749), Expect = 6.8e-194
Identity = 338/513 (65.89%), Postives = 405/513 (78.95%), Query Frame = 0

Query: 9   VITTLALAATSFVILSVLYDAVSRYWWTPRRIKKKMAMQGVGGPKPRPFLGNILHVTALL 68
           ++    L     +IL VLYD++  Y+ TPRRIKK M  QG+ GPKPR   GNI+ ++ +L
Sbjct: 5   LVLKYVLVIVMTLILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNIIDISKML 64

Query: 69  SNSTSQDMPSISHDLVPRLLPHYLVWTTQFGKRFIFWNGIEPRMCLAETDLIKELLSKHS 128
           S+S S D  SI H++VPRLLPHY+ W+ Q+GKRFI WNG EPR+CL ET++IKELL+KH+
Sbjct: 65  SHSASNDCSSIHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTETEMIKELLTKHN 124

Query: 129 AVTGKSWLQQQGSNHFIGRGLLMANGQNWFHQRHMVAPAFLGNRLKSYAGYMVECTREML 188
            VTGKSWLQQQG+  FIGRGLLMANG+ W HQRHM APAF  +RLK YA +MVECT+ M 
Sbjct: 125 PVTGKSWLQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKHMVECTKMMA 184

Query: 189 ISLENEVKSGRTEFEIGECMTRITADIISKTEFGSSYEKGKQIFHLLTTLQNLCAQASRH 248
             L  EV     E EIGE M R+TADIIS+TEFGSS +KGK++F LLT LQ LCAQA+RH
Sbjct: 185 ERLRKEVGE---EVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQRLCAQATRH 244

Query: 249 LCLPGSRFFPSKYNREIKALKGKMEGLLMEIIESRKECVE------NGNDLLGMLLNEMQ 308
           LC PGSRF PSKYNREIK+LK ++E LLMEII+SRK+ VE       G+DLLG+LLN+M 
Sbjct: 245 LCFPGSRFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLGLLLNQMD 304

Query: 309 NKRDNKGFNLNLQIIMDECKTFFFAGHETTALLLTWTLMLLATNPSWQHKVRAEVMAVCN 368
           + ++    NLN+Q+IMDECKTFFF GHETT+LLLTWTLMLLA NP+WQ  VR EV  VC 
Sbjct: 305 SNKN----NLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDNVRDEVRQVCG 364

Query: 369 RQ-APSLDHLPKLSLLGKVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVLA 428
           +   PS++ L  L+ L KVINESLRLYPPA++LPRMAFEDIKLGDL IPKGLSIWIPVLA
Sbjct: 365 QDGVPSVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPKGLSIWIPVLA 424

Query: 429 IHHSEELWGKDANEFNPERFESSKAFTSGRFIPFASGPRNCVGQSFAVMETKIILAMLIA 488
           IHHS ELWG+DANEFNPERF +    +S  F+PFA+GPRNC+GQ+FA+ME KIILAML++
Sbjct: 425 IHHSNELWGEDANEFNPERFTTRSFASSRHFMPFAAGPRNCIGQTFAMMEAKIILAMLVS 484

Query: 489 KFSFTISPNYRHAPVVVLTIKPKYGVQVCLTPV 515
           KFSF IS NYRHAP+VVLTIKPKYGVQ+ L P+
Sbjct: 485 KFSFAISENYRHAPIVVLTIKPKYGVQLVLKPL 510

BLAST of Carg05931 vs. ExPASy Swiss-Prot
Match: F4IK45 (Cytochrome P450 709B2 OS=Arabidopsis thaliana OX=3702 GN=CYP709B2 PE=2 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 8.2e-99
Identity = 196/516 (37.98%), Postives = 299/516 (57.95%), Query Frame = 0

Query: 9   VITTLALAATSFVILSV--LYDAVSRYWWTPRRIKKKMAMQGVGGPKPRPFLGNILHVTA 68
           +++T+ L A + V+L V  +Y A     W P  + ++   QG+ GPK R   GN+  +  
Sbjct: 58  LLSTINLLAIALVLLVVPKIYGACRILVWRPWMLSRRFKKQGISGPKYRILYGNLREIRK 117

Query: 69  LLSNSTSQDMPSISHDLVPRLLPHYLVWTTQFGKRFIFWNGIEPRMCLAETDLIKELLSK 128
           + + +    +   S+D+VPR+LPH   W +Q+G+ F++W G +PR+C+++ +L K++LS 
Sbjct: 118 MKNEAKLMVLDPNSNDIVPRVLPHLQQWKSQYGETFLYWQGTDPRLCISDHELAKQILSN 177

Query: 129 HSAVTGKSWLQQQGSNHFIGRGLLMANGQNWFHQRHMVAPAFLGNRLKSYAGYMVECTRE 188
                 KS  + +      G GL+  NG +W   R ++ PAF  ++LK     MV+CT  
Sbjct: 178 KFVFFSKSKTKPE-ILKLSGNGLIFVNGLDWVRHRRILNPAFSMDKLKLMTQLMVDCTFR 237

Query: 189 MLISLENEVKSGRTE--FEIGECMTRITADIISKTEFGSSYEKGKQIFHLLTTLQNLCAQ 248
           M +  + +     TE    I     R+TADII+   FGSSY +G ++F     LQ  CA 
Sbjct: 238 MFLEWKKQRNGVETEQFVLISREFKRLTADIIATAAFGSSYAEGIEVFKSQLELQKCCAA 297

Query: 249 ASRHLCLPGSRFFPSKYNREIKALKGKMEGLLMEIIESR--KECVENGNDLLGMLLNEMQ 308
           A   L  PG ++ P+  N +I  L  K+   +  II++R   E  + GNDLLG++L    
Sbjct: 298 ALTDLYFPGIQYLPTPSNLQIWKLDMKVNSSIKRIIDARLTSESKDYGNDLLGIMLTAAS 357

Query: 309 NKRDNKGFNLNLQIIMDECKTFFFAGHETTALLLTWTLMLLATNPSWQHKVRAEVMAVCN 368
           +    K   +++  I++ECKTFFFAGHETTA LLTW+ MLL+ +  WQ K+R EV   C 
Sbjct: 358 SNESEK--KMSIDEIIEECKTFFFAGHETTANLLTWSTMLLSLHQDWQEKLREEVFNECG 417

Query: 369 R-QAPSLDHLPKLSLLGKVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVLA 428
           + + P  +   KL L+  V  ESLRLY P   L R+A ED+KLG+LEIPKG +I +P+  
Sbjct: 418 KDKIPDAETCSKLKLMNTVFMESLRLYGPVLNLLRLASEDMKLGNLEIPKGTTIILPIAK 477

Query: 429 IHHSEELWGKDANEFNPERFE---SSKAFTSGRFIPFASGPRNCVGQSFAVMETKIILAM 488
           +H  + +WG DA++FNP RF    S  A      + F+ GPR C+GQ+FA+ME K +LAM
Sbjct: 478 MHRDKAVWGSDADKFNPMRFANGLSRAANHPNALLAFSMGPRACIGQNFAIMEAKTVLAM 537

Query: 489 LIAKFSFTISPNYRHAPVVVLTIKPKYGVQVCLTPV 515
           ++ +F   +S +Y+HAP   LT++P+Y + V L P+
Sbjct: 538 ILQRFRLNLSADYKHAPADHLTLQPQYDLPVILEPI 570

BLAST of Carg05931 vs. ExPASy Swiss-Prot
Match: O48786 (Cytochrome P450 734A1 OS=Arabidopsis thaliana OX=3702 GN=CYP734A1 PE=2 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 3.8e-96
Identity = 186/518 (35.91%), Postives = 300/518 (57.92%), Query Frame = 0

Query: 19  SFVILSVLYDAVSRYWWTPRRIKKKMAMQGVGGPKPRPFLGNILHVTALLSNSTSQDMPS 78
           S ++  V+   +S  WW PR+I++  + QG+ GP    F+GN+  +  ++  ++S  MP 
Sbjct: 17  SVILSLVIVKGMSLLWWRPRKIEEHFSKQGIRGPPYHFFIGNVKELVGMMLKASSHPMP- 76

Query: 79  ISHDLVPRLLPHYLVWTTQFGKRFIFWNGIEPRMCLAETDLIKELLSKHSAVTGKSWLQQ 138
            SH+++PR+L  Y  W   +G  F+ W G   R+ +A+ DLI+E+ S       KS   +
Sbjct: 77  FSHNILPRVLSFYHHWRKIYGATFLVWFGPTFRLTVADPDLIREIFS-------KSEFYE 136

Query: 139 QGSNHFI-----GRGLLMANGQNWFHQRHMVAPAFLGNRLKSYAGYMVECTREMLISLEN 198
           +   H +     G GLL   G+ W H R +++P F    LK     +++   +M+    +
Sbjct: 137 KNEAHPLVKQLEGDGLLSLKGEKWAHHRKIISPTFHMENLKLLVPVVLKSVTDMVDKWSD 196

Query: 199 EV-KSGRTEFEIGECMTRITADIISKTEFGSSYEKGKQIFHLLTTLQNLCAQASRHLCLP 258
           ++ ++G  E ++ E    +T D+IS+T FGSSYE G+ +F L      LCA+A + + +P
Sbjct: 197 KLSENGEVEVDVYEWFQILTEDVISRTAFGSSYEDGRAVFRLQAQQMLLCAEAFQKVFIP 256

Query: 259 GSRFFPSKYNREIKALKGKMEGLLMEIIESRK---------ECVE-NGNDLLGMLLNEMQ 318
           G RFFP++ N +   L  ++   L+++IE R+         EC E    DLLG+++    
Sbjct: 257 GYRFFPTRGNLKSWKLDKEIRKSLLKLIERRRQNAIDGEGEECKEPAAKDLLGLMI---- 316

Query: 319 NKRDNKGFNLNLQIIMDECKTFFFAGHETTALLLTWTLMLLATNPSWQHKVRAEVMAVC- 378
                +  N+ +Q I++ECK+FFFAG +TT+ LLTWT +LL+ +P WQ K R EV+ VC 
Sbjct: 317 -----QAKNVTVQDIVEECKSFFFAGKQTTSNLLTWTTILLSMHPEWQAKARDEVLRVCG 376

Query: 379 NRQAPSLDHLPKLSLLGKVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVLA 438
           +R  P+ DH+ KL  L  ++NESLRLYPP     R A  D+KLG  +IP G  + IP++A
Sbjct: 377 SRDVPTKDHVVKLKTLSMILNESLRLYPPIVATIRRAKSDVKLGGYKIPCGTELLIPIIA 436

Query: 439 IHHSEELWGKDANEFNPERFESSKAFTSGR---FIPFASGPRNCVGQSFAVMETKIILAM 498
           +HH + +WG D NEFNP RF       +     FIPF  G R C+GQ+ A+++ K+ LA+
Sbjct: 437 VHHDQAIWGNDVNEFNPARFADGVPRAAKHPVGFIPFGLGVRTCIGQNLAILQAKLTLAV 496

Query: 499 LIAKFSFTISPNYRHAPVVVLTIKPKYGVQVCLTPVTS 517
           +I +F+F ++P Y+HAP V++ + P++G  +    +T+
Sbjct: 497 MIQRFTFHLAPTYQHAPTVLMLLYPQHGAPITFRRLTN 517

BLAST of Carg05931 vs. ExPASy Swiss-Prot
Match: Q9ASR3 (Cytochrome P450 709B1 OS=Arabidopsis thaliana OX=3702 GN=CYP709B1 PE=2 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 7.2e-95
Identity = 191/517 (36.94%), Postives = 294/517 (56.87%), Query Frame = 0

Query: 9   VITTLALAATSFVILSVLYDAVSRYWWTPRRIKKKMAMQGVGGPKPRPFLGNILHVTALL 68
           ++  LAL     +I   ++ A     W P  + +++  QG+ GP  R F GN+  +  + 
Sbjct: 3   LVIFLALIVLILIIGLRIFKAFMILVWHPFVLTRRLKNQGISGPNYRIFYGNLSEIKKMK 62

Query: 69  SNSTSQDMPSISHDLVPRLLPHYLVWTTQFGKRFIFWNGIEPRMCLAETDLIKELLSKHS 128
             S    +   S+D+ PR+LPHY  W +Q+G+ F++WNG EPR+C+++ +L K +LS   
Sbjct: 63  RESHLSILDPSSNDIFPRILPHYQKWMSQYGETFLYWNGTEPRICISDPELAKTMLSNKL 122

Query: 129 AVTGKSWLQQQGSNHFIGRGLLMANGQNWFHQRHMVAPAFLGNRLKSYAGYMVECTREML 188
               KS  + +       +GL+   G +W   R ++ PAF  +RLK     MV+CT +ML
Sbjct: 123 GFFVKSKARPEAVKLVGSKGLVFIEGADWVRHRRILNPAFSIDRLKIMTTVMVDCTLKML 182

Query: 189 ISLENEVKSGRTEF-----EIGECMTRITADIISKTEFGSSYEKGKQIFHLLTTLQNLCA 248
                E     TE      E+ E   R+TADII+ + FGSSY +G ++F     L+    
Sbjct: 183 EEWRKESTKEETEHPKIKKEMNEEFQRLTADIIATSAFGSSYVEGIEVFRSQMELKRCYT 242

Query: 249 QASRHLCLPGSRFFPSKYNREIKALKGKMEGLLMEIIESRKECVEN-GNDLLGMLLNEMQ 308
            +   + +PG+++ P+  N  +  L+ KM+  +  II SR +   + G+DLLG+LL    
Sbjct: 243 TSLNQVSIPGTQYLPTPSNIRVWKLERKMDNSIKRIISSRLQSKSDYGDDLLGILLKAYN 302

Query: 309 NKRDNKGFNLNLQIIMDECKTFFFAGHETTALLLTWTLMLLATNPSWQHKVRAEVMAVCN 368
              + K   ++++ I+ EC+TFFF GHETT+ LL WT MLL+ +  WQ K+R E+   C 
Sbjct: 303 T--EGKERKMSIEEIIHECRTFFFGGHETTSNLLAWTTMLLSLHQDWQEKLREEIFKECG 362

Query: 369 RQ-APSLDHLPKLSLLGKVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVLA 428
           ++  P  +   KL L+  VI ESLRLY P S L R A  +IKLGDLEIPKG ++ IP+L 
Sbjct: 363 KEKTPDSETFSKLKLMNMVIMESLRLYGPVSALAREASVNIKLGDLEIPKGTTVVIPLLK 422

Query: 429 IHHSEELWGKDANEFNPERFE---SSKAFTSGRFIPFASGPRNCVGQSFAVMETKIILAM 488
           +H  + LWG DA++FNP RF    S  A      + F+ GPR C+GQ+F ++E K +L M
Sbjct: 423 MHSDKTLWGSDADKFNPMRFANGVSRAANHPNALLAFSVGPRACIGQNFVMIEAKTVLTM 482

Query: 489 LIAKFSF-TISPNYRHAPVVVLTIKPKYGVQVCLTPV 515
           ++ +F F ++   Y+H PV  +TI+P+YG+ V L P+
Sbjct: 483 ILQRFRFISLCDEYKHTPVDNVTIQPQYGLPVMLQPL 517

BLAST of Carg05931 vs. ExPASy TrEMBL
Match: A0A6J1GRP1 (cytokinin hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111456891 PE=3 SV=1)

HSP 1 Score: 1045.4 bits (2702), Expect = 7.8e-302
Identity = 518/521 (99.42%), Postives = 519/521 (99.62%), Query Frame = 0

Query: 1   MAAFILPLVITTLALAATSFVILSVLYDAVSRYWWTPRRIKKKMAMQGVGGPKPRPFLGN 60
           MAAFILPLVITTLALAATSFVILSVLYDAVS YWWTPRRIKKKMAMQGVGGPKPRPFLGN
Sbjct: 1   MAAFILPLVITTLALAATSFVILSVLYDAVSCYWWTPRRIKKKMAMQGVGGPKPRPFLGN 60

Query: 61  ILHVTALLSNSTSQDMPSISHDLVPRLLPHYLVWTTQFGKRFIFWNGIEPRMCLAETDLI 120
           ILHVTALLSNSTSQDMPS+SHDLVPRLLPHYLVWT QFGKRFIFWNGIEPRMCLAETDLI
Sbjct: 61  ILHVTALLSNSTSQDMPSVSHDLVPRLLPHYLVWTAQFGKRFIFWNGIEPRMCLAETDLI 120

Query: 121 KELLSKHSAVTGKSWLQQQGSNHFIGRGLLMANGQNWFHQRHMVAPAFLGNRLKSYAGYM 180
           KELLSKHSAVTGKSWLQQQGSNHFIGRGLLMANGQNWFHQRHMVAPAFLGNRLKSYAGYM
Sbjct: 121 KELLSKHSAVTGKSWLQQQGSNHFIGRGLLMANGQNWFHQRHMVAPAFLGNRLKSYAGYM 180

Query: 181 VECTREMLISLENEVKSGRTEFEIGECMTRITADIISKTEFGSSYEKGKQIFHLLTTLQN 240
           VECTREMLISLENEVKSGRTEFEIGECMTRITADIISKTEFGSSYEKGKQIFHLLTTLQN
Sbjct: 181 VECTREMLISLENEVKSGRTEFEIGECMTRITADIISKTEFGSSYEKGKQIFHLLTTLQN 240

Query: 241 LCAQASRHLCLPGSRFFPSKYNREIKALKGKMEGLLMEIIESRKECVENGNDLLGMLLNE 300
           LCAQASRHLCLPGSRFFPSKYNREIKALKGKMEGLLMEIIESRKECVENGNDLLGMLLNE
Sbjct: 241 LCAQASRHLCLPGSRFFPSKYNREIKALKGKMEGLLMEIIESRKECVENGNDLLGMLLNE 300

Query: 301 MQNKRDNKGFNLNLQIIMDECKTFFFAGHETTALLLTWTLMLLATNPSWQHKVRAEVMAV 360
           MQNKRDNKGFNLNLQIIMDECKTFFFAGHETTALLLTWTLMLLATNPSWQHKVRAEVMAV
Sbjct: 301 MQNKRDNKGFNLNLQIIMDECKTFFFAGHETTALLLTWTLMLLATNPSWQHKVRAEVMAV 360

Query: 361 CNRQAPSLDHLPKLSLLGKVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVL 420
           CNRQAPSLDHLPKLSLLGKVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVL
Sbjct: 361 CNRQAPSLDHLPKLSLLGKVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVL 420

Query: 421 AIHHSEELWGKDANEFNPERFESSKAFTSGRFIPFASGPRNCVGQSFAVMETKIILAMLI 480
           AIHHSEELWGKDANEFNPERFESSKAFTSGRFIPFASGPRNCVGQSFAVMETKIILAMLI
Sbjct: 421 AIHHSEELWGKDANEFNPERFESSKAFTSGRFIPFASGPRNCVGQSFAVMETKIILAMLI 480

Query: 481 AKFSFTISPNYRHAPVVVLTIKPKYGVQVCLTPVTSSLVGL 522
           AKFSFTISPNYRHAPVVVLTIKPKYGVQVCLTPVTSSLVGL
Sbjct: 481 AKFSFTISPNYRHAPVVVLTIKPKYGVQVCLTPVTSSLVGL 521

BLAST of Carg05931 vs. ExPASy TrEMBL
Match: A0A6J1JM26 (cytokinin hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111487109 PE=3 SV=1)

HSP 1 Score: 983.0 bits (2540), Expect = 4.7e-283
Identity = 492/514 (95.72%), Postives = 497/514 (96.69%), Query Frame = 0

Query: 1   MAAFILPLVITTLALAATSFVILSVLYDAVSRYWWTPRRIKKKMAMQGVGGPKPRPFLGN 60
           MAAFILPLVITTLALAATSFVILSVLYDAVS YW TPRRIKKKMAMQGVGGPKPRPFLGN
Sbjct: 1   MAAFILPLVITTLALAATSFVILSVLYDAVSCYWSTPRRIKKKMAMQGVGGPKPRPFLGN 60

Query: 61  ILHVTALLSNSTSQDMPSISHDLVPRLLPHYLVWTTQFGKRFIFWNGIEPRMCLAETDLI 120
           IL VTALLS STSQDMPSISHDLVPRLLPHYLVWT QFGKRFIFWNGIEPRMCLAETDLI
Sbjct: 61  ILDVTALLSKSTSQDMPSISHDLVPRLLPHYLVWTAQFGKRFIFWNGIEPRMCLAETDLI 120

Query: 121 KELLSKHSAVTGKSWLQQQGSNHFIGRGLLMANGQNWFHQRHMVAPAFLGNRLKSYAGYM 180
           KELLSKHSAVTGKSWLQQQGS+HFIGRGLLMANGQNWFHQRH+VAPAFLGNRLKSYAG M
Sbjct: 121 KELLSKHSAVTGKSWLQQQGSSHFIGRGLLMANGQNWFHQRHVVAPAFLGNRLKSYAGCM 180

Query: 181 VECTREMLISLENEVKSGRTEFEIGECMTRITADIISKTEFGSSYEKGKQIFHLLTTLQN 240
           VECTREMLISLENEVK GRTEFEIGE MTRITADIISKTEFGSSYEKGKQIFHLLTTLQN
Sbjct: 181 VECTREMLISLENEVKLGRTEFEIGEYMTRITADIISKTEFGSSYEKGKQIFHLLTTLQN 240

Query: 241 LCAQASRHLCLPGSRFFPSKYNREIKALKGKMEGLLMEIIESRKECVENGNDLLGMLLNE 300
           LCAQASRHLCLPGSRFFPSKYNREIKALKGK+EGLLMEIIESRKECVENGNDLLGMLLNE
Sbjct: 241 LCAQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIESRKECVENGNDLLGMLLNE 300

Query: 301 MQNKRDNKGFNLNLQIIMDECKTFFFAGHETTALLLTWTLMLLATNPSWQHKVRAEVMAV 360
           MQNKRDNKGFNLNLQIIMDECKTFFFAGHETTALLLTWTLMLLATNPSWQ KVRAEVMAV
Sbjct: 301 MQNKRDNKGFNLNLQIIMDECKTFFFAGHETTALLLTWTLMLLATNPSWQDKVRAEVMAV 360

Query: 361 CNRQAPSLDHLPKLSLLGKVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVL 420
           CNRQAPSLDHLPKLS L  VINESLRLYPPASILPR+AFEDIKLGDLEIPKGLSIWIPVL
Sbjct: 361 CNRQAPSLDHLPKLSQLSMVINESLRLYPPASILPRIAFEDIKLGDLEIPKGLSIWIPVL 420

Query: 421 AIHHSEELWGKDANEFNPERFESSKAFTSGRFIPFASGPRNCVGQSFAVMETKIILAMLI 480
           AIHHSEELWGKDANEF PERF SSK FTSGRFIPFASGPRNCVGQSFAVMETKIILAMLI
Sbjct: 421 AIHHSEELWGKDANEFKPERFGSSKVFTSGRFIPFASGPRNCVGQSFAVMETKIILAMLI 480

Query: 481 AKFSFTISPNYRHAPVVVLTIKPKYGVQVCLTPV 515
           AKFSF IS NYRHAPVVVLTIKPKYGVQVCLTP+
Sbjct: 481 AKFSFAISQNYRHAPVVVLTIKPKYGVQVCLTPL 514

BLAST of Carg05931 vs. ExPASy TrEMBL
Match: A0A6J1FGF0 (cytokinin hydroxylase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445453 PE=3 SV=1)

HSP 1 Score: 874.0 bits (2257), Expect = 3.1e-250
Identity = 432/519 (83.24%), Postives = 468/519 (90.17%), Query Frame = 0

Query: 3   AFILPLVITTLALAATSFVILSVLYDAVSRYWWTPRRIKKKMAMQGVGGPKPRPFLGNIL 62
           A +L L++TTLA AATSF I S+LY  +S YW TPRRI +KMA QGV GPKPRP LGNIL
Sbjct: 2   AIMLALLLTTLAFAATSFCIFSLLYHTLSCYWLTPRRIMRKMAKQGVRGPKPRPLLGNIL 61

Query: 63  HVTALLSNSTSQDMPSISHDLVPRLLPHYLVWTTQFGKRFIFWNGIEPRMCLAETDLIKE 122
            V ALLS STS DMPSISHDLVPRLLPHYL W+ QFGKRFIFWNGIEPRMCL ET LIKE
Sbjct: 62  DVAALLSKSTSSDMPSISHDLVPRLLPHYLAWSAQFGKRFIFWNGIEPRMCLTETHLIKE 121

Query: 123 LLSKHSAVTGKSWLQQQGSNHFIGRGLLMANGQNWFHQRHMVAPAFLGNRLKSYAGYMVE 182
           LLSK+S+V+GKSWLQQQGS HFIGRGLLMANG NWFHQRH+VAPAF+G+RLKSYAGYMVE
Sbjct: 122 LLSKYSSVSGKSWLQQQGSKHFIGRGLLMANGPNWFHQRHIVAPAFVGDRLKSYAGYMVE 181

Query: 183 CTREMLISLENEVKSGRTEFEIGECMTRITADIISKTEFGSSYEKGKQIFHLLTTLQNLC 242
           CTREML SLENEVKSGR+EFEIGE MTR+TADIIS+TEF SSYEKGKQIFHLLT LQ+LC
Sbjct: 182 CTREMLQSLENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLC 241

Query: 243 AQASRHLCLPGSRFFPSKYNREIKALKGKMEGLLMEIIESRKECVE------NGNDLLGM 302
           AQASRHLCLPGSRFFPSKYNREIKALKGK+EGLLMEII+SRK+ VE       GNDLLGM
Sbjct: 242 AQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRKDGVEIGRSSSYGNDLLGM 301

Query: 303 LLNEMQNKRDNKGFNLNLQIIMDECKTFFFAGHETTALLLTWTLMLLATNPSWQHKVRAE 362
           LLNEMQ K++  G +LNLQIIMDECKTFFFAGHETTALLLTWT+MLLATNPSWQ +VRAE
Sbjct: 302 LLNEMQKKKNGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTIMLLATNPSWQDRVRAE 361

Query: 363 VMAVCNRQAPSLDHLPKLSLLGKVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIW 422
           + AVCN Q PSL HL KL LLG VINESLRLYPPASILPRMAFEDIKLGDLEIPKGLS+W
Sbjct: 362 LNAVCNSQTPSLHHLSKLPLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSVW 421

Query: 423 IPVLAIHHSEELWGKDANEFNPERFESSKAFTSGRFIPFASGPRNCVGQSFAVMETKIIL 482
           IPV+AIHHSEE+WGKDANEFNPERF SSKAFT G FIPFASGPRNCVGQ+FA+METKIIL
Sbjct: 422 IPVVAIHHSEEIWGKDANEFNPERFGSSKAFTCGGFIPFASGPRNCVGQAFALMETKIIL 481

Query: 483 AMLIAKFSFTISPNYRHAPVVVLTIKPKYGVQVCLTPVT 516
           AMLI+KF FTISP+YRHAPVVVLT+KPKYGVQ+CLTP+T
Sbjct: 482 AMLISKFRFTISPSYRHAPVVVLTVKPKYGVQICLTPLT 520

BLAST of Carg05931 vs. ExPASy TrEMBL
Match: A0A6J1JYD2 (cytokinin hydroxylase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111488993 PE=3 SV=1)

HSP 1 Score: 871.7 bits (2251), Expect = 1.5e-249
Identity = 431/519 (83.04%), Postives = 467/519 (89.98%), Query Frame = 0

Query: 3   AFILPLVITTLALAATSFVILSVLYDAVSRYWWTPRRIKKKMAMQGVGGPKPRPFLGNIL 62
           A +L L++TTLA A TSF I S+LY  +S YW TPRRI +KMA QGV GPKPRP LGNIL
Sbjct: 2   ATMLALLLTTLAFAVTSFCIFSLLYHTLSCYWLTPRRIMRKMAKQGVRGPKPRPLLGNIL 61

Query: 63  HVTALLSNSTSQDMPSISHDLVPRLLPHYLVWTTQFGKRFIFWNGIEPRMCLAETDLIKE 122
            V ALLS STS DMPSISHDLVPRLLPHYL W+ QFGKRFIFWNGIEPRMCL ET LIKE
Sbjct: 62  DVAALLSKSTSSDMPSISHDLVPRLLPHYLAWSAQFGKRFIFWNGIEPRMCLTETHLIKE 121

Query: 123 LLSKHSAVTGKSWLQQQGSNHFIGRGLLMANGQNWFHQRHMVAPAFLGNRLKSYAGYMVE 182
           LLSK+S+V+GKSWLQQQGS HFIGRGLLMANG NWFHQRH+VAPAF+G+RLKSYAGYMVE
Sbjct: 122 LLSKYSSVSGKSWLQQQGSKHFIGRGLLMANGPNWFHQRHIVAPAFVGDRLKSYAGYMVE 181

Query: 183 CTREMLISLENEVKSGRTEFEIGECMTRITADIISKTEFGSSYEKGKQIFHLLTTLQNLC 242
           CTREML SLENEVKSGR+EFEIGE MTR+TADIIS+TEF SSYEKGKQIFHLLT LQ+LC
Sbjct: 182 CTREMLQSLENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLC 241

Query: 243 AQASRHLCLPGSRFFPSKYNREIKALKGKMEGLLMEIIESRKECVE------NGNDLLGM 302
           AQASRHLCLPGSRFFPSKYNREIKALKGK+EGLLMEII+SRK+ VE       GNDLLGM
Sbjct: 242 AQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRKDGVEIGRSSSYGNDLLGM 301

Query: 303 LLNEMQNKRDNKGFNLNLQIIMDECKTFFFAGHETTALLLTWTLMLLATNPSWQHKVRAE 362
           LLNEMQ K++  G +LNLQIIMDECKTFFFAGHETTALLLTWT+MLLATNPSWQ +VRAE
Sbjct: 302 LLNEMQKKKNGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTIMLLATNPSWQDRVRAE 361

Query: 363 VMAVCNRQAPSLDHLPKLSLLGKVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIW 422
           + AVCN Q PSL HL KL LLG VINESLRLYPPASILPRMAFEDIKLGDLEIPKGLS+W
Sbjct: 362 LNAVCNSQTPSLHHLSKLPLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSVW 421

Query: 423 IPVLAIHHSEELWGKDANEFNPERFESSKAFTSGRFIPFASGPRNCVGQSFAVMETKIIL 482
           IPV+AIHHSEE+WGKDANEFNPERF SSKAFT G FIPFASGPRNCVGQ+FA+METKIIL
Sbjct: 422 IPVVAIHHSEEIWGKDANEFNPERFGSSKAFTCGGFIPFASGPRNCVGQAFALMETKIIL 481

Query: 483 AMLIAKFSFTISPNYRHAPVVVLTIKPKYGVQVCLTPVT 516
           AMLI+KF FTISP+YRHAPVVVLT+KPKYGVQ+CLTP+T
Sbjct: 482 AMLISKFRFTISPSYRHAPVVVLTVKPKYGVQICLTPLT 520

BLAST of Carg05931 vs. ExPASy TrEMBL
Match: A0A5A7T7B8 (Cytokinin hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold64G001740 PE=3 SV=1)

HSP 1 Score: 865.9 bits (2236), Expect = 8.5e-248
Identity = 431/518 (83.20%), Postives = 465/518 (89.77%), Query Frame = 0

Query: 5   ILPLVITTLALAATSFVILSVLYDAVSRYWWTPRRIKKKMAMQGVGGPKPRPFLGNILHV 64
           +LPL++TTLAL  TSF I S+LYD VS Y  TPRRIK+KMA QGV GPKPRP LGNIL V
Sbjct: 4   MLPLLLTTLALLTTSFFIFSLLYDTVSCYLLTPRRIKQKMAKQGVHGPKPRPLLGNILDV 63

Query: 65  TALLSNSTSQDMPSISHDLVPRLLPHYLVWTTQFGKRFIFWNGIEPRMCLAETDLIKELL 124
            +LLS STS DMPSISHDLVPRLLPH+L W++QFGKRFIFWNGIEPRMCL ETDLIKELL
Sbjct: 64  ASLLSKSTSSDMPSISHDLVPRLLPHFLAWSSQFGKRFIFWNGIEPRMCLTETDLIKELL 123

Query: 125 SKHSAVTGKSWLQQQGSNHFIGRGLLMANGQNWFHQRHMVAPAFLGNRLKSYAGYMVECT 184
           SK+S V+GKSWLQQQGS HFIGRGLLMANGQNWFHQRH+VAPAF+G+RLKSYAGYMVECT
Sbjct: 124 SKYSTVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVECT 183

Query: 185 REMLISLENEVKSGRTEFEIGECMTRITADIISKTEFGSSYEKGKQIFHLLTTLQNLCAQ 244
           +EML S+E EVKSGR+EFEIGE MTR+TADIIS+TEF SS+EKGKQIFHLLT LQ+LCAQ
Sbjct: 184 KEMLESIEKEVKSGRSEFEIGEYMTRLTADIISRTEFESSFEKGKQIFHLLTVLQHLCAQ 243

Query: 245 ASRHLCLPGSRFFPSKYNREIKALKGKMEGLLMEIIESRKECVE------NGNDLLGMLL 304
           ASRHLC PGSRFFPSKYNREIKALKGK+E LLMEII+SRK+CVE       GNDLLGMLL
Sbjct: 244 ASRHLCFPGSRFFPSKYNREIKALKGKVEELLMEIIQSRKDCVEIGRSSSYGNDLLGMLL 303

Query: 305 NEMQNKR--DNKGFNLNLQIIMDECKTFFFAGHETTALLLTWTLMLLATNPSWQHKVRAE 364
           NEMQ K+   N G +LNLQIIMDECKTFFFAGHETTALLLTWT+MLLATNP+WQ KVRAE
Sbjct: 304 NEMQKKKLDGNNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPTWQDKVRAE 363

Query: 365 VMAVCNRQAPSLDHLPKLSLLGKVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIW 424
           VMAVC  + PS  HL KLSLL  VINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIW
Sbjct: 364 VMAVCGSETPSFHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIW 423

Query: 425 IPVLAIHHSEELWGKDANEFNPERFESSKAFTSGRFIPFASGPRNCVGQSFAVMETKIIL 484
           IPVLAIHHSEELWGKDANEFNPERF +SK FTSG FIPFASGPRNC+GQSFA+METKIIL
Sbjct: 424 IPVLAIHHSEELWGKDANEFNPERFANSKPFTSGGFIPFASGPRNCIGQSFALMETKIIL 483

Query: 485 AMLIAKFSFTISPNYRHAPVVVLTIKPKYGVQVCLTPV 515
           AMLI+KFSFTIS NYRHAPVVVLTIKPKYGVQVCL P+
Sbjct: 484 AMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPL 521

BLAST of Carg05931 vs. TAIR 10
Match: AT5G38450.1 (cytochrome P450, family 735, subfamily A, polypeptide 1 )

HSP 1 Score: 708.8 bits (1828), Expect = 3.3e-204
Identity = 352/519 (67.82%), Postives = 416/519 (80.15%), Query Frame = 0

Query: 8   LVITTLALAATSFV--ILSVLYDAVSRYWWTPRRIKKKMAMQGVGGPKPRPFLGNILHVT 67
           +++T L      FV  IL VLYD +S YW TPRRIKK M  QGV GPKPRP  GNIL ++
Sbjct: 1   MLLTILKSLLVIFVTTILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEIS 60

Query: 68  ALLSNSTSQDMPSISHDLVPRLLPHYLVWTTQFGKRFIFWNGIEPRMCLAETDLIKELLS 127
           A++S S S+D  SI HD+V RLLPHY+ W+ Q+GKRFI WNG +PR+CL ET+LIKELL 
Sbjct: 61  AMVSQSASKDCDSIHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELIKELLM 120

Query: 128 KHSAVTGKSWLQQQGSNHFIGRGLLMANGQNWFHQRHMVAPAFLGNRLKSYAGYMVECTR 187
           KH+ V+G+SWLQQQG+ +FIGRGLLMANGQ+W HQRH+ APAF G RLK YA +MVECT 
Sbjct: 121 KHNGVSGRSWLQQQGTKNFIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHMVECTS 180

Query: 188 EMLISLENEVKSGRTEFEIGECMTRITADIISKTEFGSSYEKGKQIFHLLTTLQNLCAQA 247
           +++  L  EV  G  E EIGE M ++TADIIS+T+FGSS+EKGK++F+ LT LQ  CAQA
Sbjct: 181 KLVERLRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRRCAQA 240

Query: 248 SRHLCLPGSRFFPSKYNREIKALKGKMEGLLMEIIESRKECVE------NGNDLLGMLLN 307
           +RHLC PGSRF PSKYNREIK+LK ++E LL+EII+SR++C E      +G+DLLG+LLN
Sbjct: 241 TRHLCFPGSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDDLLGLLLN 300

Query: 308 EMQNKRDNKGFNLNLQIIMDECKTFFFAGHETTALLLTWTLMLLATNPSWQHKVRAEVMA 367
           EM   ++N   N NLQ+IMDECKTFFFAGHETTALLLTWT MLLA NP+WQ KVR EV  
Sbjct: 301 EMDIDKNNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEKVREEVRE 360

Query: 368 VCNRQA-PSLDHLPKLSLLGKVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIP 427
           V  R   PS+D L KL+ L KVINESLRLYPPA++LPRMAFED+KLGDL IPKGLSIWIP
Sbjct: 361 VFGRNGLPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPKGLSIWIP 420

Query: 428 VLAIHHSEELWGKDANEFNPERFESSKAFTSGR-FIPFASGPRNCVGQSFAVMETKIILA 487
           VLAIHHSEELWGKDAN+FNPERF   + F SGR FIPFA+GPRNC+GQ FA+ME KIILA
Sbjct: 421 VLAIHHSEELWGKDANQFNPERF-GGRPFASGRHFIPFAAGPRNCIGQQFALMEAKIILA 480

Query: 488 MLIAKFSFTISPNYRHAPVVVLTIKPKYGVQVCLTPVTS 517
            LI+KF+FTIS NYRHAP+VVLTIKPKYGVQV L P+ S
Sbjct: 481 TLISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPLVS 518

BLAST of Carg05931 vs. TAIR 10
Match: AT1G67110.1 (cytochrome P450, family 735, subfamily A, polypeptide 2 )

HSP 1 Score: 678.3 bits (1749), Expect = 4.8e-195
Identity = 338/513 (65.89%), Postives = 405/513 (78.95%), Query Frame = 0

Query: 9   VITTLALAATSFVILSVLYDAVSRYWWTPRRIKKKMAMQGVGGPKPRPFLGNILHVTALL 68
           ++    L     +IL VLYD++  Y+ TPRRIKK M  QG+ GPKPR   GNI+ ++ +L
Sbjct: 5   LVLKYVLVIVMTLILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNIIDISKML 64

Query: 69  SNSTSQDMPSISHDLVPRLLPHYLVWTTQFGKRFIFWNGIEPRMCLAETDLIKELLSKHS 128
           S+S S D  SI H++VPRLLPHY+ W+ Q+GKRFI WNG EPR+CL ET++IKELL+KH+
Sbjct: 65  SHSASNDCSSIHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTETEMIKELLTKHN 124

Query: 129 AVTGKSWLQQQGSNHFIGRGLLMANGQNWFHQRHMVAPAFLGNRLKSYAGYMVECTREML 188
            VTGKSWLQQQG+  FIGRGLLMANG+ W HQRHM APAF  +RLK YA +MVECT+ M 
Sbjct: 125 PVTGKSWLQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKHMVECTKMMA 184

Query: 189 ISLENEVKSGRTEFEIGECMTRITADIISKTEFGSSYEKGKQIFHLLTTLQNLCAQASRH 248
             L  EV     E EIGE M R+TADIIS+TEFGSS +KGK++F LLT LQ LCAQA+RH
Sbjct: 185 ERLRKEVGE---EVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQRLCAQATRH 244

Query: 249 LCLPGSRFFPSKYNREIKALKGKMEGLLMEIIESRKECVE------NGNDLLGMLLNEMQ 308
           LC PGSRF PSKYNREIK+LK ++E LLMEII+SRK+ VE       G+DLLG+LLN+M 
Sbjct: 245 LCFPGSRFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLGLLLNQMD 304

Query: 309 NKRDNKGFNLNLQIIMDECKTFFFAGHETTALLLTWTLMLLATNPSWQHKVRAEVMAVCN 368
           + ++    NLN+Q+IMDECKTFFF GHETT+LLLTWTLMLLA NP+WQ  VR EV  VC 
Sbjct: 305 SNKN----NLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDNVRDEVRQVCG 364

Query: 369 RQ-APSLDHLPKLSLLGKVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVLA 428
           +   PS++ L  L+ L KVINESLRLYPPA++LPRMAFEDIKLGDL IPKGLSIWIPVLA
Sbjct: 365 QDGVPSVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPKGLSIWIPVLA 424

Query: 429 IHHSEELWGKDANEFNPERFESSKAFTSGRFIPFASGPRNCVGQSFAVMETKIILAMLIA 488
           IHHS ELWG+DANEFNPERF +    +S  F+PFA+GPRNC+GQ+FA+ME KIILAML++
Sbjct: 425 IHHSNELWGEDANEFNPERFTTRSFASSRHFMPFAAGPRNCIGQTFAMMEAKIILAMLVS 484

Query: 489 KFSFTISPNYRHAPVVVLTIKPKYGVQVCLTPV 515
           KFSF IS NYRHAP+VVLTIKPKYGVQ+ L P+
Sbjct: 485 KFSFAISENYRHAPIVVLTIKPKYGVQLVLKPL 510

BLAST of Carg05931 vs. TAIR 10
Match: AT2G46950.1 (cytochrome P450, family 709, subfamily B, polypeptide 2 )

HSP 1 Score: 362.5 bits (929), Expect = 5.8e-100
Identity = 196/516 (37.98%), Postives = 299/516 (57.95%), Query Frame = 0

Query: 9   VITTLALAATSFVILSV--LYDAVSRYWWTPRRIKKKMAMQGVGGPKPRPFLGNILHVTA 68
           +++T+ L A + V+L V  +Y A     W P  + ++   QG+ GPK R   GN+  +  
Sbjct: 58  LLSTINLLAIALVLLVVPKIYGACRILVWRPWMLSRRFKKQGISGPKYRILYGNLREIRK 117

Query: 69  LLSNSTSQDMPSISHDLVPRLLPHYLVWTTQFGKRFIFWNGIEPRMCLAETDLIKELLSK 128
           + + +    +   S+D+VPR+LPH   W +Q+G+ F++W G +PR+C+++ +L K++LS 
Sbjct: 118 MKNEAKLMVLDPNSNDIVPRVLPHLQQWKSQYGETFLYWQGTDPRLCISDHELAKQILSN 177

Query: 129 HSAVTGKSWLQQQGSNHFIGRGLLMANGQNWFHQRHMVAPAFLGNRLKSYAGYMVECTRE 188
                 KS  + +      G GL+  NG +W   R ++ PAF  ++LK     MV+CT  
Sbjct: 178 KFVFFSKSKTKPE-ILKLSGNGLIFVNGLDWVRHRRILNPAFSMDKLKLMTQLMVDCTFR 237

Query: 189 MLISLENEVKSGRTE--FEIGECMTRITADIISKTEFGSSYEKGKQIFHLLTTLQNLCAQ 248
           M +  + +     TE    I     R+TADII+   FGSSY +G ++F     LQ  CA 
Sbjct: 238 MFLEWKKQRNGVETEQFVLISREFKRLTADIIATAAFGSSYAEGIEVFKSQLELQKCCAA 297

Query: 249 ASRHLCLPGSRFFPSKYNREIKALKGKMEGLLMEIIESR--KECVENGNDLLGMLLNEMQ 308
           A   L  PG ++ P+  N +I  L  K+   +  II++R   E  + GNDLLG++L    
Sbjct: 298 ALTDLYFPGIQYLPTPSNLQIWKLDMKVNSSIKRIIDARLTSESKDYGNDLLGIMLTAAS 357

Query: 309 NKRDNKGFNLNLQIIMDECKTFFFAGHETTALLLTWTLMLLATNPSWQHKVRAEVMAVCN 368
           +    K   +++  I++ECKTFFFAGHETTA LLTW+ MLL+ +  WQ K+R EV   C 
Sbjct: 358 SNESEK--KMSIDEIIEECKTFFFAGHETTANLLTWSTMLLSLHQDWQEKLREEVFNECG 417

Query: 369 R-QAPSLDHLPKLSLLGKVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVLA 428
           + + P  +   KL L+  V  ESLRLY P   L R+A ED+KLG+LEIPKG +I +P+  
Sbjct: 418 KDKIPDAETCSKLKLMNTVFMESLRLYGPVLNLLRLASEDMKLGNLEIPKGTTIILPIAK 477

Query: 429 IHHSEELWGKDANEFNPERFE---SSKAFTSGRFIPFASGPRNCVGQSFAVMETKIILAM 488
           +H  + +WG DA++FNP RF    S  A      + F+ GPR C+GQ+FA+ME K +LAM
Sbjct: 478 MHRDKAVWGSDADKFNPMRFANGLSRAANHPNALLAFSMGPRACIGQNFAIMEAKTVLAM 537

Query: 489 LIAKFSFTISPNYRHAPVVVLTIKPKYGVQVCLTPV 515
           ++ +F   +S +Y+HAP   LT++P+Y + V L P+
Sbjct: 538 ILQRFRLNLSADYKHAPADHLTLQPQYDLPVILEPI 570

BLAST of Carg05931 vs. TAIR 10
Match: AT2G26710.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 353.6 bits (906), Expect = 2.7e-97
Identity = 186/518 (35.91%), Postives = 300/518 (57.92%), Query Frame = 0

Query: 19  SFVILSVLYDAVSRYWWTPRRIKKKMAMQGVGGPKPRPFLGNILHVTALLSNSTSQDMPS 78
           S ++  V+   +S  WW PR+I++  + QG+ GP    F+GN+  +  ++  ++S  MP 
Sbjct: 17  SVILSLVIVKGMSLLWWRPRKIEEHFSKQGIRGPPYHFFIGNVKELVGMMLKASSHPMP- 76

Query: 79  ISHDLVPRLLPHYLVWTTQFGKRFIFWNGIEPRMCLAETDLIKELLSKHSAVTGKSWLQQ 138
            SH+++PR+L  Y  W   +G  F+ W G   R+ +A+ DLI+E+ S       KS   +
Sbjct: 77  FSHNILPRVLSFYHHWRKIYGATFLVWFGPTFRLTVADPDLIREIFS-------KSEFYE 136

Query: 139 QGSNHFI-----GRGLLMANGQNWFHQRHMVAPAFLGNRLKSYAGYMVECTREMLISLEN 198
           +   H +     G GLL   G+ W H R +++P F    LK     +++   +M+    +
Sbjct: 137 KNEAHPLVKQLEGDGLLSLKGEKWAHHRKIISPTFHMENLKLLVPVVLKSVTDMVDKWSD 196

Query: 199 EV-KSGRTEFEIGECMTRITADIISKTEFGSSYEKGKQIFHLLTTLQNLCAQASRHLCLP 258
           ++ ++G  E ++ E    +T D+IS+T FGSSYE G+ +F L      LCA+A + + +P
Sbjct: 197 KLSENGEVEVDVYEWFQILTEDVISRTAFGSSYEDGRAVFRLQAQQMLLCAEAFQKVFIP 256

Query: 259 GSRFFPSKYNREIKALKGKMEGLLMEIIESRK---------ECVE-NGNDLLGMLLNEMQ 318
           G RFFP++ N +   L  ++   L+++IE R+         EC E    DLLG+++    
Sbjct: 257 GYRFFPTRGNLKSWKLDKEIRKSLLKLIERRRQNAIDGEGEECKEPAAKDLLGLMI---- 316

Query: 319 NKRDNKGFNLNLQIIMDECKTFFFAGHETTALLLTWTLMLLATNPSWQHKVRAEVMAVC- 378
                +  N+ +Q I++ECK+FFFAG +TT+ LLTWT +LL+ +P WQ K R EV+ VC 
Sbjct: 317 -----QAKNVTVQDIVEECKSFFFAGKQTTSNLLTWTTILLSMHPEWQAKARDEVLRVCG 376

Query: 379 NRQAPSLDHLPKLSLLGKVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVLA 438
           +R  P+ DH+ KL  L  ++NESLRLYPP     R A  D+KLG  +IP G  + IP++A
Sbjct: 377 SRDVPTKDHVVKLKTLSMILNESLRLYPPIVATIRRAKSDVKLGGYKIPCGTELLIPIIA 436

Query: 439 IHHSEELWGKDANEFNPERFESSKAFTSGR---FIPFASGPRNCVGQSFAVMETKIILAM 498
           +HH + +WG D NEFNP RF       +     FIPF  G R C+GQ+ A+++ K+ LA+
Sbjct: 437 VHHDQAIWGNDVNEFNPARFADGVPRAAKHPVGFIPFGLGVRTCIGQNLAILQAKLTLAV 496

Query: 499 LIAKFSFTISPNYRHAPVVVLTIKPKYGVQVCLTPVTS 517
           +I +F+F ++P Y+HAP V++ + P++G  +    +T+
Sbjct: 497 MIQRFTFHLAPTYQHAPTVLMLLYPQHGAPITFRRLTN 517

BLAST of Carg05931 vs. TAIR 10
Match: AT2G46960.2 (cytochrome P450, family 709, subfamily B, polypeptide 1 )

HSP 1 Score: 349.4 bits (895), Expect = 5.1e-96
Identity = 191/517 (36.94%), Postives = 294/517 (56.87%), Query Frame = 0

Query: 9   VITTLALAATSFVILSVLYDAVSRYWWTPRRIKKKMAMQGVGGPKPRPFLGNILHVTALL 68
           ++  LAL     +I   ++ A     W P  + +++  QG+ GP  R F GN+  +  + 
Sbjct: 3   LVIFLALIVLILIIGLRIFKAFMILVWHPFVLTRRLKNQGISGPNYRIFYGNLSEIKKMK 62

Query: 69  SNSTSQDMPSISHDLVPRLLPHYLVWTTQFGKRFIFWNGIEPRMCLAETDLIKELLSKHS 128
             S    +   S+D+ PR+LPHY  W +Q+G+ F++WNG EPR+C+++ +L K +LS   
Sbjct: 63  RESHLSILDPSSNDIFPRILPHYQKWMSQYGETFLYWNGTEPRICISDPELAKTMLSNKL 122

Query: 129 AVTGKSWLQQQGSNHFIGRGLLMANGQNWFHQRHMVAPAFLGNRLKSYAGYMVECTREML 188
               KS  + +       +GL+   G +W   R ++ PAF  +RLK     MV+CT +ML
Sbjct: 123 GFFVKSKARPEAVKLVGSKGLVFIEGADWVRHRRILNPAFSIDRLKIMTTVMVDCTLKML 182

Query: 189 ISLENEVKSGRTEF-----EIGECMTRITADIISKTEFGSSYEKGKQIFHLLTTLQNLCA 248
                E     TE      E+ E   R+TADII+ + FGSSY +G ++F     L+    
Sbjct: 183 EEWRKESTKEETEHPKIKKEMNEEFQRLTADIIATSAFGSSYVEGIEVFRSQMELKRCYT 242

Query: 249 QASRHLCLPGSRFFPSKYNREIKALKGKMEGLLMEIIESRKECVEN-GNDLLGMLLNEMQ 308
            +   + +PG+++ P+  N  +  L+ KM+  +  II SR +   + G+DLLG+LL    
Sbjct: 243 TSLNQVSIPGTQYLPTPSNIRVWKLERKMDNSIKRIISSRLQSKSDYGDDLLGILLKAYN 302

Query: 309 NKRDNKGFNLNLQIIMDECKTFFFAGHETTALLLTWTLMLLATNPSWQHKVRAEVMAVCN 368
              + K   ++++ I+ EC+TFFF GHETT+ LL WT MLL+ +  WQ K+R E+   C 
Sbjct: 303 T--EGKERKMSIEEIIHECRTFFFGGHETTSNLLAWTTMLLSLHQDWQEKLREEIFKECG 362

Query: 369 RQ-APSLDHLPKLSLLGKVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVLA 428
           ++  P  +   KL L+  VI ESLRLY P S L R A  +IKLGDLEIPKG ++ IP+L 
Sbjct: 363 KEKTPDSETFSKLKLMNMVIMESLRLYGPVSALAREASVNIKLGDLEIPKGTTVVIPLLK 422

Query: 429 IHHSEELWGKDANEFNPERFE---SSKAFTSGRFIPFASGPRNCVGQSFAVMETKIILAM 488
           +H  + LWG DA++FNP RF    S  A      + F+ GPR C+GQ+F ++E K +L M
Sbjct: 423 MHSDKTLWGSDADKFNPMRFANGVSRAANHPNALLAFSVGPRACIGQNFVMIEAKTVLTM 482

Query: 489 LIAKFSF-TISPNYRHAPVVVLTIKPKYGVQVCLTPV 515
           ++ +F F ++   Y+H PV  +TI+P+YG+ V L P+
Sbjct: 483 ILQRFRFISLCDEYKHTPVDNVTIQPQYGLPVMLQPL 517