Carg05912 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg05912 vs. NCBI nr
Match: KAG7033235.1 (Proline dehydrogenase 2, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 941.0 bits (2431), Expect = 3.9e-270
Identity = 477/477 (100.00%), Postives = 477/477 (100.00%), Query Frame = 0

Query: 1   MARRILATQSKTPKRFFIYTFDRLLNTAASSPAAAFTAAAASASPLNHTGSPATPTIDFT 60
           MARRILATQSKTPKRFFIYTFDRLLNTAASSPAAAFTAAAASASPLNHTGSPATPTIDFT
Sbjct: 1   MARRILATQSKTPKRFFIYTFDRLLNTAASSPAAAFTAAAASASPLNHTGSPATPTIDFT 60

Query: 61  DTSALFGSLSTLELLRAAANLHLAAVGPVVDAGIWIMNSKLMDVELIRDAILGSVKHTFY 120
           DTSALFGSLSTLELLRAAANLHLAAVGPVVDAGIWIMNSKLMDVELIRDAILGSVKHTFY
Sbjct: 61  DTSALFGSLSTLELLRAAANLHLAAVGPVVDAGIWIMNSKLMDVELIRDAILGSVKHTFY 120

Query: 121 RHFCAGEDDPAVRKTVRRLHDVGLRSMLDYALEYADDEESCDRNLDGFLKTIEATKSLPP 180
           RHFCAGEDDPAVRKTVRRLHDVGLRSMLDYALEYADDEESCDRNLDGFLKTIEATKSLPP
Sbjct: 121 RHFCAGEDDPAVRKTVRRLHDVGLRSMLDYALEYADDEESCDRNLDGFLKTIEATKSLPP 180

Query: 181 GSASFVVAKVSAICPLRLLERVSDLLRWQHKNPCLNLPWKLKTLPIFSDSSPLYHTLKRP 240
           GSASFVVAKVSAICPLRLLERVSDLLRWQHKNPCLNLPWKLKTLPIFSDSSPLYHTLKRP
Sbjct: 181 GSASFVVAKVSAICPLRLLERVSDLLRWQHKNPCLNLPWKLKTLPIFSDSSPLYHTLKRP 240

Query: 241 PPLTPEEEKSLQISHQRLIKICQACADANVPLAIDAEHTKVQPAIDYFTYSAAIDYNKDH 300
           PPLTPEEEKSLQISHQRLIKICQACADANVPLAIDAEHTKVQPAIDYFTYSAAIDYNKDH
Sbjct: 241 PPLTPEEEKSLQISHQRLIKICQACADANVPLAIDAEHTKVQPAIDYFTYSAAIDYNKDH 300

Query: 301 NPIVYGTVQAYLKDAKDRLLLVTKAADNMKIPLGIKLVRGAYMSTESKLASSLGFPSPIH 360
           NPIVYGTVQAYLKDAKDRLLLVTKAADNMKIPLGIKLVRGAYMSTESKLASSLGFPSPIH
Sbjct: 301 NPIVYGTVQAYLKDAKDRLLLVTKAADNMKIPLGIKLVRGAYMSTESKLASSLGFPSPIH 360

Query: 361 DTILDTHSCYDGCASFLLDNVVKSSSGVILATHNVESGKLAATKAYEIGIGKLKHKLEFA 420
           DTILDTHSCYDGCASFLLDNVVKSSSGVILATHNVESGKLAATKAYEIGIGKLKHKLEFA
Sbjct: 361 DTILDTHSCYDGCASFLLDNVVKSSSGVILATHNVESGKLAATKAYEIGIGKLKHKLEFA 420

Query: 421 QLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRKLM 478
           QLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRKLM
Sbjct: 421 QLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRKLM 477

BLAST of Carg05912 vs. NCBI nr
Match: KAG6602556.1 (Proline dehydrogenase 2, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 931.4 bits (2406), Expect = 3.1e-267
Identity = 472/477 (98.95%), Postives = 474/477 (99.37%), Query Frame = 0

Query: 1   MARRILATQSKTPKRFFIYTFDRLLNTAASSPAAAFTAAAASASPLNHTGSPATPTIDFT 60
           MARRILATQSKTPKRFF+YTFDRLLNTAASSPAAAFTAAAASASPLNHTGSPATPTIDFT
Sbjct: 1   MARRILATQSKTPKRFFLYTFDRLLNTAASSPAAAFTAAAASASPLNHTGSPATPTIDFT 60

Query: 61  DTSALFGSLSTLELLRAAANLHLAAVGPVVDAGIWIMNSKLMDVELIRDAILGSVKHTFY 120
           DTSALFGSLSTLELLRAAANLHLAAVGPVVDAGIWIMNSKLMDVELIRD ILGSVKHTFY
Sbjct: 61  DTSALFGSLSTLELLRAAANLHLAAVGPVVDAGIWIMNSKLMDVELIRDTILGSVKHTFY 120

Query: 121 RHFCAGEDDPAVRKTVRRLHDVGLRSMLDYALEYADDEESCDRNLDGFLKTIEATKSLPP 180
           RHFCAGEDD AVRKTVRRLHDVGLRSMLDYALEYADDEESCDRNLDGFLKTIEATKSLPP
Sbjct: 121 RHFCAGEDDAAVRKTVRRLHDVGLRSMLDYALEYADDEESCDRNLDGFLKTIEATKSLPP 180

Query: 181 GSASFVVAKVSAICPLRLLERVSDLLRWQHKNPCLNLPWKLKTLPIFSDSSPLYHTLKRP 240
           GSASFVVAKVSAICPLRLLERVSDLLRWQHKNPCLNLPWKLKTLPIFSDSSPLYHTLKRP
Sbjct: 181 GSASFVVAKVSAICPLRLLERVSDLLRWQHKNPCLNLPWKLKTLPIFSDSSPLYHTLKRP 240

Query: 241 PPLTPEEEKSLQISHQRLIKICQACADANVPLAIDAEHTKVQPAIDYFTYSAAIDYNKDH 300
           PPLTPEEEKSLQISHQRLIKICQACADANVPLAIDAEHTKVQPAIDYFTYSAAIDYNKDH
Sbjct: 241 PPLTPEEEKSLQISHQRLIKICQACADANVPLAIDAEHTKVQPAIDYFTYSAAIDYNKDH 300

Query: 301 NPIVYGTVQAYLKDAKDRLLLVTKAADNMKIPLGIKLVRGAYMSTESKLASSLGFPSPIH 360
           NPIVYGTVQAYLKDAKDRLLLVTKAADNMKIPLGIKLVRGAYMS+ESKLASSLGFPSPIH
Sbjct: 301 NPIVYGTVQAYLKDAKDRLLLVTKAADNMKIPLGIKLVRGAYMSSESKLASSLGFPSPIH 360

Query: 361 DTILDTHSCYDGCASFLLDNVVKSSSGVILATHNVESGKLAATKAYEIGIGKLKHKLEFA 420
           DTILDTHSCYDGCAS LLDNVVKSSSGVILATHNVESGKLAATKAYEIGIGKLKHKLEFA
Sbjct: 361 DTILDTHSCYDGCASVLLDNVVKSSSGVILATHNVESGKLAATKAYEIGIGKLKHKLEFA 420

Query: 421 QLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRKLM 478
           QLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRKLM
Sbjct: 421 QLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRKLM 477

BLAST of Carg05912 vs. NCBI nr
Match: XP_022923652.1 (proline dehydrogenase 2, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 927.5 bits (2396), Expect = 4.5e-266
Identity = 470/477 (98.53%), Postives = 473/477 (99.16%), Query Frame = 0

Query: 1   MARRILATQSKTPKRFFIYTFDRLLNTAASSPAAAFTAAAASASPLNHTGSPATPTIDFT 60
           MARRILATQSKTPKRFF+YTFDRLLNTAASSPAAAFTAAAASASPLNHTGSPATPTIDFT
Sbjct: 1   MARRILATQSKTPKRFFLYTFDRLLNTAASSPAAAFTAAAASASPLNHTGSPATPTIDFT 60

Query: 61  DTSALFGSLSTLELLRAAANLHLAAVGPVVDAGIWIMNSKLMDVELIRDAILGSVKHTFY 120
           DTSALFGSLSTLELLRAAANLHLAAVGPVVDAGIWIMNSKLMDVELIRDAILGSVKHTFY
Sbjct: 61  DTSALFGSLSTLELLRAAANLHLAAVGPVVDAGIWIMNSKLMDVELIRDAILGSVKHTFY 120

Query: 121 RHFCAGEDDPAVRKTVRRLHDVGLRSMLDYALEYADDEESCDRNLDGFLKTIEATKSLPP 180
           RHFCAGEDD AVRKTVRRLHDVGLRSMLDYALEYADDEESCDRNLDGFLKTIEATKSLPP
Sbjct: 121 RHFCAGEDDAAVRKTVRRLHDVGLRSMLDYALEYADDEESCDRNLDGFLKTIEATKSLPP 180

Query: 181 GSASFVVAKVSAICPLRLLERVSDLLRWQHKNPCLNLPWKLKTLPIFSDSSPLYHTLKRP 240
           GSASFVVAKVSAICPLRLLERVSDLLRWQHKNPCLNLPWKLKTLPIFSDSSPLYHTLK P
Sbjct: 181 GSASFVVAKVSAICPLRLLERVSDLLRWQHKNPCLNLPWKLKTLPIFSDSSPLYHTLKHP 240

Query: 241 PPLTPEEEKSLQISHQRLIKICQACADANVPLAIDAEHTKVQPAIDYFTYSAAIDYNKDH 300
            PLTP+EEKSLQISHQRLIKICQACADANVPLAIDAEHTKVQPAIDYFTYSAAIDYNKDH
Sbjct: 241 TPLTPQEEKSLQISHQRLIKICQACADANVPLAIDAEHTKVQPAIDYFTYSAAIDYNKDH 300

Query: 301 NPIVYGTVQAYLKDAKDRLLLVTKAADNMKIPLGIKLVRGAYMSTESKLASSLGFPSPIH 360
           NPIVYGTVQAYLKDAKDRLLLV KAADNMKIPLGIKLVRGAYMS+ESKLASSLGFPSPIH
Sbjct: 301 NPIVYGTVQAYLKDAKDRLLLVAKAADNMKIPLGIKLVRGAYMSSESKLASSLGFPSPIH 360

Query: 361 DTILDTHSCYDGCASFLLDNVVKSSSGVILATHNVESGKLAATKAYEIGIGKLKHKLEFA 420
           DTILDTHSCYDGCASFLLDNVVKSSSGVILATHNVESGKLAATKAYEIGIGKLKHKLEFA
Sbjct: 361 DTILDTHSCYDGCASFLLDNVVKSSSGVILATHNVESGKLAATKAYEIGIGKLKHKLEFA 420

Query: 421 QLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRKLM 478
           QLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRKLM
Sbjct: 421 QLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRKLM 477

BLAST of Carg05912 vs. NCBI nr
Match: XP_023524135.1 (proline dehydrogenase 2, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 905.6 bits (2339), Expect = 1.8e-259
Identity = 461/477 (96.65%), Postives = 467/477 (97.90%), Query Frame = 0

Query: 1   MARRILATQSKTPKRFFIYTFDRLLNTAASSPAAAFTAAAASASPLNHTGSPATPTIDFT 60
           MARRILATQSKTPKRFF+YTFDRLLNTAASSPAAAFTAAA  ASPLN TGSPA PT+DFT
Sbjct: 1   MARRILATQSKTPKRFFLYTFDRLLNTAASSPAAAFTAAA--ASPLNLTGSPAKPTVDFT 60

Query: 61  DTSALFGSLSTLELLRAAANLHLAAVGPVVDAGIWIMNSKLMDVELIRDAILGSVKHTFY 120
           DTSALFGSLSTLELLRAAANLHLAAVGPVVDAGIWIMNSKLMDVELIR+AILG+VKHTFY
Sbjct: 61  DTSALFGSLSTLELLRAAANLHLAAVGPVVDAGIWIMNSKLMDVELIREAILGTVKHTFY 120

Query: 121 RHFCAGEDDPAVRKTVRRLHDVGLRSMLDYALEYADDEESCDRNLDGFLKTIEATKSLPP 180
           RHFCAGEDD AVRKTVRRLHDVGLRSMLDYALEYADDEESCDRNLDGFLKTIEATKSLPP
Sbjct: 121 RHFCAGEDDAAVRKTVRRLHDVGLRSMLDYALEYADDEESCDRNLDGFLKTIEATKSLPP 180

Query: 181 GSASFVVAKVSAICPLRLLERVSDLLRWQHKNPCLNLPWKLKTLPIFSDSSPLYHTLKRP 240
           GSASFVVAKVSAICPLRLLERVSDLLRWQHKNPCLNLPWKLKTLPIFSDSSPLYHTLK P
Sbjct: 181 GSASFVVAKVSAICPLRLLERVSDLLRWQHKNPCLNLPWKLKTLPIFSDSSPLYHTLKHP 240

Query: 241 PPLTPEEEKSLQISHQRLIKICQACADANVPLAIDAEHTKVQPAIDYFTYSAAIDYNKDH 300
            PLTP+EEKSLQISHQRLIKICQACADANVPLAIDAEHTKVQPAIDYFTYSAAIDYNKDH
Sbjct: 241 TPLTPQEEKSLQISHQRLIKICQACADANVPLAIDAEHTKVQPAIDYFTYSAAIDYNKDH 300

Query: 301 NPIVYGTVQAYLKDAKDRLLLVTKAADNMKIPLGIKLVRGAYMSTESKLASSLGFPSPIH 360
           NPIVYGTVQAYLKDAKDRLLLV KAADNMKIPLGIKLVRGAYMS+ESKLASSLGF SPIH
Sbjct: 301 NPIVYGTVQAYLKDAKDRLLLVAKAADNMKIPLGIKLVRGAYMSSESKLASSLGFSSPIH 360

Query: 361 DTILDTHSCYDGCASFLLDNVVKSSSGVILATHNVESGKLAATKAYEIGIGKLKHKLEFA 420
           DTILDTHSCYDGCASFLLDNVVKSSSGVILATHNVESGKLAA KAYEIGIGKLKHKLEFA
Sbjct: 361 DTILDTHSCYDGCASFLLDNVVKSSSGVILATHNVESGKLAAAKAYEIGIGKLKHKLEFA 420

Query: 421 QLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRKLM 478
           QLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRKLM
Sbjct: 421 QLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRKLM 475

BLAST of Carg05912 vs. NCBI nr
Match: XP_022990936.1 (proline dehydrogenase 2, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 902.1 bits (2330), Expect = 2.0e-258
Identity = 460/477 (96.44%), Postives = 466/477 (97.69%), Query Frame = 0

Query: 1   MARRILATQSKTPKRFFIYTFDRLLNTAASSPAAAFTAAAASASPLNHTGSPATPTIDFT 60
           MARRILATQSKTPKRFF+YTFDRLLNTAASSPAAAFT  AASASPLN TGSPATPT+DFT
Sbjct: 1   MARRILATQSKTPKRFFLYTFDRLLNTAASSPAAAFT--AASASPLNLTGSPATPTVDFT 60

Query: 61  DTSALFGSLSTLELLRAAANLHLAAVGPVVDAGIWIMNSKLMDVELIRDAILGSVKHTFY 120
           DTSALFGSLSTLELLRAAANLHLAAVGPVVDAGIWIMNSKLMDVELIR+AILGSVKHTFY
Sbjct: 61  DTSALFGSLSTLELLRAAANLHLAAVGPVVDAGIWIMNSKLMDVELIREAILGSVKHTFY 120

Query: 121 RHFCAGEDDPAVRKTVRRLHDVGLRSMLDYALEYADDEESCDRNLDGFLKTIEATKSLPP 180
           RHFCAGEDD AVRKTVRRLHDVGLRSMLDYALEYADDEESCDRNLDGFLKTIEA KSLPP
Sbjct: 121 RHFCAGEDDAAVRKTVRRLHDVGLRSMLDYALEYADDEESCDRNLDGFLKTIEAIKSLPP 180

Query: 181 GSASFVVAKVSAICPLRLLERVSDLLRWQHKNPCLNLPWKLKTLPIFSDSSPLYHTLKRP 240
           GSASFVVAKVSAICPLRLLERVSDLLRWQHKNPCLNLPWK KTLPIFSDSSPLYHTLK P
Sbjct: 181 GSASFVVAKVSAICPLRLLERVSDLLRWQHKNPCLNLPWKFKTLPIFSDSSPLYHTLKHP 240

Query: 241 PPLTPEEEKSLQISHQRLIKICQACADANVPLAIDAEHTKVQPAIDYFTYSAAIDYNKDH 300
            PLTPEEEKSLQISHQRL+KICQA ADANVPLAIDAEHTKVQPAIDYFTYSAAIDYNKDH
Sbjct: 241 TPLTPEEEKSLQISHQRLMKICQASADANVPLAIDAEHTKVQPAIDYFTYSAAIDYNKDH 300

Query: 301 NPIVYGTVQAYLKDAKDRLLLVTKAADNMKIPLGIKLVRGAYMSTESKLASSLGFPSPIH 360
           NPIVYGTVQAYLKDAKDRLLLVTKAADNMKIPLGIKLVRGAYMS+ESKLA SLGFPSPIH
Sbjct: 301 NPIVYGTVQAYLKDAKDRLLLVTKAADNMKIPLGIKLVRGAYMSSESKLALSLGFPSPIH 360

Query: 361 DTILDTHSCYDGCASFLLDNVVKSSSGVILATHNVESGKLAATKAYEIGIGKLKHKLEFA 420
           DTILDTHSCY+GCASFLLDNVVKSSSGVILATHNVESGKLAA KAYEIGIGKLKHKLEFA
Sbjct: 361 DTILDTHSCYNGCASFLLDNVVKSSSGVILATHNVESGKLAAAKAYEIGIGKLKHKLEFA 420

Query: 421 QLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRKLM 478
           QLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRKLM
Sbjct: 421 QLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRKLM 475

BLAST of Carg05912 vs. ExPASy Swiss-Prot
Match: P92983 (Proline dehydrogenase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=POX1 PE=1 SV=2)

HSP 1 Score: 496.9 bits (1278), Expect = 2.6e-139
Identity = 244/424 (57.55%), Postives = 316/424 (74.53%), Query Frame = 0

Query: 57  IDFTDTSALFGSLSTLELLRAAANLHLAAVGPVVDAGIWIMNSKLMDVELIRDAILGSVK 116
           +D +D + LF S+ T +LLR+ A LH AA+GP+VD G W+M+SKLMD  + R  +LG VK
Sbjct: 63  LDLSDQARLFSSIPTSDLLRSTAVLHAAAIGPMVDLGTWVMSSKLMDASVTRGMVLGLVK 122

Query: 117 HTFYRHFCAGEDDPAVRKTVRRLHD-VGLRSMLDYALEYADDEESCDRNLDGFLKTIEAT 176
            TFY HFCAGED  A  + VR +++  GL+ ML Y +E+ADD  SCD N+  F++TIEA 
Sbjct: 123 STFYDHFCAGEDADAAAERVRSVYEATGLKGMLVYGVEHADDAVSCDDNMQQFIRTIEAA 182

Query: 177 KSLPPGSASFVVAKVSAICPLRLLERVSDLLRWQHKNPCLNLPWKLKTLPIFSDSSPLYH 236
           KSLP    S VV K++AICP+ LL+RVSDLLRW++K+P   L WKLK+ P+FS+SSPLYH
Sbjct: 183 KSLPTSHFSSVVVKITAICPISLLKRVSDLLRWEYKSPNFKLSWKLKSFPVFSESSPLYH 242

Query: 237 TLKRPPPLTPEEEKSLQISHQRLIKICQACADANVPLAIDAEHTKVQPAIDYFTYSAAID 296
           T   P PLT EEE+ L+ +H R+ +IC+ C ++NVPL IDAE T +QPAIDY  YS+AI 
Sbjct: 243 TNSEPEPLTAEEERELEAAHGRIQEICRKCQESNVPLLIDAEDTILQPAIDYMAYSSAIM 302

Query: 297 YNKDHN-PIVYGTVQAYLKDAKDRLLLVTKAADNMKIPLGIKLVRGAYMSTESKLASSLG 356
           +N D + PIVY T+QAYL+DA +RL L  + A+   +P+G KLVRGAYMS+E+ LA SLG
Sbjct: 303 FNADKDRPIVYNTIQAYLRDAGERLHLAVQNAEKENVPMGFKLVRGAYMSSEASLADSLG 362

Query: 357 FPSPIHDTILDTHSCYDGCASFLLDNVVKSSS-GVILATHNVESGKLAATKAYEIGIGKL 416
             SP+HDTI DTHSCY+ C +FL++     S  GV+LATHN +SG+LA+ KA ++GI K 
Sbjct: 363 CKSPVHDTIQDTHSCYNDCMTFLMEKASNGSGFGVVLATHNADSGRLASRKASDLGIDKQ 422

Query: 417 KHKLEFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLD 476
             K+EFAQLYGMS+ALSFGL+ AGF VSKYMPFGPV   +PYLLRRA ENRG+++T   D
Sbjct: 423 NGKIEFAQLYGMSDALSFGLKRAGFNVSKYMPFGPVATAIPYLLRRAYENRGMMATGAHD 482

Query: 477 RKLM 478
           R+LM
Sbjct: 483 RQLM 486

BLAST of Carg05912 vs. ExPASy Swiss-Prot
Match: Q6NKX1 (Proline dehydrogenase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=POX2 PE=2 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 3.1e-137
Identity = 239/424 (56.37%), Postives = 310/424 (73.11%), Query Frame = 0

Query: 57  IDFTDTSALFGSLSTLELLRAAANLHLAAVGPVVDAGIWIMNSKLMDVELIRDAILGSVK 116
           +D +D + LF S+    LLR+ A LH  ++GP+VD G W+M+SKLMD  + RD +L  VK
Sbjct: 42  LDLSDQARLFASVPISTLLRSTAILHATSIGPMVDLGSWLMSSKLMDTTVTRDLVLRIVK 101

Query: 117 HTFYRHFCAGEDDPAVRKTVRRLHD-VGLRSMLDYALEYADDEESCDRNLDGFLKTIEAT 176
            TFY HFCAGED  A  + V  +++  GL+ ML Y +E+A+D  +CD N+  F++T+EA 
Sbjct: 102 GTFYDHFCAGEDAAAAARRVSSVYESTGLKGMLVYGVEHAEDGGACDENIQKFIETVEAA 161

Query: 177 KSLPPGSASFVVAKVSAICPLRLLERVSDLLRWQHKNPCLNLPWKLKTLPIFSDSSPLYH 236
           K+LP    S VV K++AICP+ +L+RVSDLLRWQ+KNP   LPWKL + P+FS  SPLYH
Sbjct: 162 KTLPSSHLSSVVVKITAICPMNVLKRVSDLLRWQYKNPNFKLPWKLNSFPVFSGLSPLYH 221

Query: 237 TLKRPPPLTPEEEKSLQISHQRLIKICQACADANVPLAIDAEHTKVQPAIDYFTYSAAID 296
           T   P PLT EEE+ L+ +H+RL  +C  C ++NVPL IDAE T +QPAIDY  Y +AI 
Sbjct: 222 TTSEPEPLTVEEERELEKAHERLKSVCLRCQESNVPLLIDAEDTILQPAIDYMAYWSAIM 281

Query: 297 YNKDHN-PIVYGTVQAYLKDAKDRLLLVTKAADNMKIPLGIKLVRGAYMSTESKLASSLG 356
           +N D + PIVY T+QAYLKDA +RL L  + ++ M +P+G KLVRGAYMS+E+KLA SLG
Sbjct: 282 FNSDKDRPIVYNTIQAYLKDAGERLHLALRESEKMNVPIGFKLVRGAYMSSEAKLADSLG 341

Query: 357 FPSPIHDTILDTHSCYDGCASFLLDNVVKSSS-GVILATHNVESGKLAATKAYEIGIGKL 416
           + SP+HDTI +TH CY+ C SFL++     S   VILATHN +SGKL A KA E+GI K 
Sbjct: 342 YKSPVHDTIQNTHDCYNDCMSFLMEKASNGSGIAVILATHNTDSGKLGARKASELGINKE 401

Query: 417 KHKLEFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLD 476
             K+EFAQLYGMS+ALSFGL+ AGF VSKYMP+GPVD  +PYL+RRA ENRG++ST  LD
Sbjct: 402 NGKIEFAQLYGMSDALSFGLKRAGFNVSKYMPYGPVDTAIPYLIRRAYENRGMMSTGALD 461

Query: 477 RKLM 478
           R+LM
Sbjct: 462 RQLM 465

BLAST of Carg05912 vs. ExPASy Swiss-Prot
Match: Q86H28 (Proline dehydrogenase 1, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=prodh PE=3 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 8.4e-50
Identity = 141/430 (32.79%), Postives = 223/430 (51.86%), Query Frame = 0

Query: 61  DTSALFGSLSTLELLRAAANLHLAAVGPVVDAGIWIMNSKLMDVELIRDAILGSVKHTFY 120
           DTS L+ S ST EL      L + ++  + D     +N  L +   +   +   +K++F+
Sbjct: 145 DTSKLYVSKSTGELFFTFMILKVCSINFISDNSQKFLN--LFEKLGLSKPLNFFIKYSFF 204

Query: 121 RHFCAGEDDPAVRKTVRRLHDVGLRSMLDYAL-EYADDEESCDRNLDGFLKTIEATKSLP 180
           + FCAGE          +L+ +G+ ++LDYA+ E A   E  D   +   +TI      P
Sbjct: 205 KQFCAGETIRETEIFTEKLNKLGIGTILDYAIEELAGSSEGFDSVAENICETIRVAAKNP 264

Query: 181 PGSASFVVAKVSAICPLRLLERVSDLLRWQHKNPCLNLPWKLKTLPIFSDSSPLYHTLKR 240
             S S V  K + +    +LE+++ L+         N+   +  LPI S +SPL   L +
Sbjct: 265 TNSFSCV--KFTGLVTPSVLEKMNTLVS--------NVTTNVSELPIESFNSPLDFYLNQ 324

Query: 241 ---------PPPLTPEEEKSLQISHQRLIKICQACADANVPLAIDAEHTKVQPAIDYFTY 300
                     P LT ++ K ++    R+ KI Q C    VP+ +DAE +  Q AI + T 
Sbjct: 325 SSSLMKQGSEPLLTSKDIKEIKEFFNRMDKIFQLCHQRGVPILVDAEQSYYQVAIHHLTM 384

Query: 301 SAAIDYNKDHNPIVYGTVQAYLKDAKDRLLLVTKAADNMK--IPLGIKLVRGAYMSTESK 360
           S +I YNK+  PI+Y T Q YL +  + L    + + + K    LG K+VRGAYM TES+
Sbjct: 385 SYSIKYNKE-KPIIYNTYQMYLVNGMNVLKQHFELSSSQKFNFKLGAKIVRGAYMVTESE 444

Query: 361 LASSLGFPSPIHDTILDTHSCYDGCASFLLDNVVK--SSSGVILATHNVESGKLAATKAY 420
            +  L   +P+  TI DTH  Y+    FLL+ +    +S G+++A+HN +S  L      
Sbjct: 445 RSQRLSTENPVLPTIQDTHKSYNTALDFLLNQIKSDPNSIGLMIASHNEDSINLGTKLIK 504

Query: 421 EIGIGKLKHKLEFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGL 477
           +  I      ++F QL+GM++ LSF L +   ++ KY+PFGPV+ V+PYL+RR  EN+G 
Sbjct: 505 QYKIDPTNPNIQFGQLFGMADFLSFNLVDQHQRIFKYVPFGPVEEVLPYLIRRMHENKGF 560

BLAST of Carg05912 vs. ExPASy Swiss-Prot
Match: O45228 (Proline dehydrogenase 1, mitochondrial OS=Caenorhabditis elegans OX=6239 GN=prdh-1 PE=2 SV=2)

HSP 1 Score: 168.3 bits (425), Expect = 2.1e-40
Identity = 147/547 (26.87%), Postives = 224/547 (40.95%), Query Frame = 0

Query: 57  IDFTDTSALFGSLSTLELLRAAANLHLAAVGPVVDAGIWIMNSKLMDVELIRDAILGSVK 116
           + F +T   F S S  EL+RA   L L  +  +V+    I+N+  M   L ++    ++K
Sbjct: 59  LSFENTKEAFKSKSNTELVRALVVLRLCGIQTLVNQNQIILNT--MRRVLGKNLFKKTLK 118

Query: 117 HTFYRHFCAGEDDPAVRKTVRRLHDVGLRSMLDYALE----------------------- 176
           +TF+ HF AGE +  VR  V +L + G++S+LDY++E                       
Sbjct: 119 NTFFGHFVAGETEEEVRHVVEKLRNYGVKSILDYSVEADITSQEATDKTVKGTSVATVKP 178

Query: 177 --------------------------------------YADDEESCDRNLDGFLKTIEAT 236
                                                 + + EE CD+N D F  +I A 
Sbjct: 179 AAMTPVVDAKTLETTRERYTVHEEFGDRRQGVSSARTYFYEGEEQCDKNRDIFKDSINAV 238

Query: 237 KSLPPGSASFVVAKVSAICPLRLLERVS-------------------------------- 296
            S    +  FV  K++A+   +LL ++S                                
Sbjct: 239 ASATK-NEGFVAVKITALGRPQLLLKLSEAIVQTQNFFKALTGGMSLQEGRLTSQEFYKR 298

Query: 297 ----------------------------DLLRWQH-KNPCLNLPWKLKTLPIFSDS-SPL 356
                                       DL  W H  +  + L    + L I + S  PL
Sbjct: 299 LGELGVKTDTESVKKFFDEVDFDSDGIVDLHGWNHILDDHVKLGQLFQVLNIKTGSLEPL 358

Query: 357 YHTLKRPPPLTPEEEKSLQISHQRLIKICQACADANVPLAIDAEHTKVQPAIDYFTYSAA 416
              L        EEE+  +   +R + + +   +  V + +DAE T +QPAI   T    
Sbjct: 359 IQNLSN------EEEQEFRNMVRRTLDVAEYAIEKGVRIMVDAEQTYLQPAISKITIEMM 418

Query: 417 IDYNKDHNPIVYGTVQAYLKDAKDRLLLVTKAADNMKIPLGIKLVRGAYMSTESKLASSL 476
             YNK    I + T QAYLK     +    + A       G KLVRGAYM  E   A ++
Sbjct: 419 KKYNKGRGNI-FNTYQAYLKGTLQNMEADMQVARREGWHFGAKLVRGAYMEQERARAKAI 478

Query: 477 GFPSPIHDTILDTHSCYDGCASFLLDNV---VKSSSGVILATHNVESGKLAATKAYEIGI 478
           G+  PI+D    T   Y+ C + + D V    K++  V++A+HN ++ + A     E  I
Sbjct: 479 GYEDPINDNFEATSKMYESCLTRIADEVHRRGKTNVSVMVASHNEDTVRFALNLMKEKCI 538

BLAST of Carg05912 vs. ExPASy Swiss-Prot
Match: A6QQ74 (Hydroxyproline dehydrogenase OS=Bos taurus OX=9913 GN=PRODH2 PE=2 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 7.4e-38
Identity = 127/421 (30.17%), Postives = 204/421 (48.46%), Query Frame = 0

Query: 73  ELLRAAANLHLAAVGPVVDAGIWIMNSKLMDVELIRDAILGS-VKHTFYRHFCAGEDDPA 132
           EL RA   L L A  P+V  G+ +   +     L+   + G+ ++ + Y  F AGE    
Sbjct: 36  ELTRALLVLRLCAWPPLVTHGLAL---QAWSQRLLGSRLSGALLRASIYGQFVAGETAEE 95

Query: 133 VRKTVRRLHDVGLRSMLDYALEYADD------EESCDRNLDGFLKTIEATKSL-----PP 192
           VR  V +L ++GLR +L    E   D      E   + NL   L+ ++ ++ L     P 
Sbjct: 96  VRSCVLQLQNLGLRPLLAVPTEEEPDSAVKTGEAWYEGNLSAMLRCVDLSRGLLETPDPT 155

Query: 193 GSASFVVAKVSAICPLRLLERVSDLLRWQHKNPCLNLPWKLKTLPIFSDSSPLYHTLKRP 252
           G+A  +  K++A+   RL ++++  +R     P  +L    + L    DS          
Sbjct: 156 GNA-LMQLKMTALMSTRLCKQLTSWVR----RPGDSLELSPERLAEAMDSGQDLQV---- 215

Query: 253 PPLTPEEEKSLQISHQRLIKICQACADANVPLAIDAEHTKVQPAIDYFTYSAAIDYNK-- 312
             L  E+ + LQ S  RL ++ Q      V L +DAE+T + PA+     + A  +N   
Sbjct: 216 SCLNTEQTRHLQASLSRLHRVVQHARAQRVRLLVDAEYTSLNPALSLLVAALATRWNSSG 275

Query: 313 DHNPIVYGTVQAYLKDAKDRLLLVTKAADNMKIPLGIKLVRGAYMSTESKLASSLGFPSP 372
           +  P V+ T QAYLKD  +RL    +AAD   +  G+KLVRGAY+  E + A   G   P
Sbjct: 276 EGGPWVWNTYQAYLKDTYERLRWDAEAADRAGLAFGVKLVRGAYLDKERETARLQGTEDP 335

Query: 373 IHDTILDTHSCYDGCASFLLDNVVKSS--SGVILATHNVESGKLAATKAYEIGIGKLKHK 432
                  T   Y  C   +L  V        +++A+HN +S + A  + +E+GI      
Sbjct: 336 TQPDYEATSQSYSRCLELMLTQVSHRGPMCHLMVASHNEDSVRQATKRMWELGIPP-DGP 395

Query: 433 LEFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRKL 478
           + F QL GM + +S  L  AG+ V K +P+G ++ V+PYL+RRA+ENR +L  +  +++L
Sbjct: 396 VCFGQLLGMCDHVSLALGQAGYAVYKSIPYGSLEEVIPYLIRRAQENRSVLRGARREQEL 443

BLAST of Carg05912 vs. ExPASy TrEMBL
Match: A0A6J1E6Q2 (Proline dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111431296 PE=3 SV=1)

HSP 1 Score: 927.5 bits (2396), Expect = 2.2e-266
Identity = 470/477 (98.53%), Postives = 473/477 (99.16%), Query Frame = 0

Query: 1   MARRILATQSKTPKRFFIYTFDRLLNTAASSPAAAFTAAAASASPLNHTGSPATPTIDFT 60
           MARRILATQSKTPKRFF+YTFDRLLNTAASSPAAAFTAAAASASPLNHTGSPATPTIDFT
Sbjct: 1   MARRILATQSKTPKRFFLYTFDRLLNTAASSPAAAFTAAAASASPLNHTGSPATPTIDFT 60

Query: 61  DTSALFGSLSTLELLRAAANLHLAAVGPVVDAGIWIMNSKLMDVELIRDAILGSVKHTFY 120
           DTSALFGSLSTLELLRAAANLHLAAVGPVVDAGIWIMNSKLMDVELIRDAILGSVKHTFY
Sbjct: 61  DTSALFGSLSTLELLRAAANLHLAAVGPVVDAGIWIMNSKLMDVELIRDAILGSVKHTFY 120

Query: 121 RHFCAGEDDPAVRKTVRRLHDVGLRSMLDYALEYADDEESCDRNLDGFLKTIEATKSLPP 180
           RHFCAGEDD AVRKTVRRLHDVGLRSMLDYALEYADDEESCDRNLDGFLKTIEATKSLPP
Sbjct: 121 RHFCAGEDDAAVRKTVRRLHDVGLRSMLDYALEYADDEESCDRNLDGFLKTIEATKSLPP 180

Query: 181 GSASFVVAKVSAICPLRLLERVSDLLRWQHKNPCLNLPWKLKTLPIFSDSSPLYHTLKRP 240
           GSASFVVAKVSAICPLRLLERVSDLLRWQHKNPCLNLPWKLKTLPIFSDSSPLYHTLK P
Sbjct: 181 GSASFVVAKVSAICPLRLLERVSDLLRWQHKNPCLNLPWKLKTLPIFSDSSPLYHTLKHP 240

Query: 241 PPLTPEEEKSLQISHQRLIKICQACADANVPLAIDAEHTKVQPAIDYFTYSAAIDYNKDH 300
            PLTP+EEKSLQISHQRLIKICQACADANVPLAIDAEHTKVQPAIDYFTYSAAIDYNKDH
Sbjct: 241 TPLTPQEEKSLQISHQRLIKICQACADANVPLAIDAEHTKVQPAIDYFTYSAAIDYNKDH 300

Query: 301 NPIVYGTVQAYLKDAKDRLLLVTKAADNMKIPLGIKLVRGAYMSTESKLASSLGFPSPIH 360
           NPIVYGTVQAYLKDAKDRLLLV KAADNMKIPLGIKLVRGAYMS+ESKLASSLGFPSPIH
Sbjct: 301 NPIVYGTVQAYLKDAKDRLLLVAKAADNMKIPLGIKLVRGAYMSSESKLASSLGFPSPIH 360

Query: 361 DTILDTHSCYDGCASFLLDNVVKSSSGVILATHNVESGKLAATKAYEIGIGKLKHKLEFA 420
           DTILDTHSCYDGCASFLLDNVVKSSSGVILATHNVESGKLAATKAYEIGIGKLKHKLEFA
Sbjct: 361 DTILDTHSCYDGCASFLLDNVVKSSSGVILATHNVESGKLAATKAYEIGIGKLKHKLEFA 420

Query: 421 QLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRKLM 478
           QLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRKLM
Sbjct: 421 QLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRKLM 477

BLAST of Carg05912 vs. ExPASy TrEMBL
Match: A0A6J1JPB7 (Proline dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111487675 PE=3 SV=1)

HSP 1 Score: 902.1 bits (2330), Expect = 9.7e-259
Identity = 460/477 (96.44%), Postives = 466/477 (97.69%), Query Frame = 0

Query: 1   MARRILATQSKTPKRFFIYTFDRLLNTAASSPAAAFTAAAASASPLNHTGSPATPTIDFT 60
           MARRILATQSKTPKRFF+YTFDRLLNTAASSPAAAFT  AASASPLN TGSPATPT+DFT
Sbjct: 1   MARRILATQSKTPKRFFLYTFDRLLNTAASSPAAAFT--AASASPLNLTGSPATPTVDFT 60

Query: 61  DTSALFGSLSTLELLRAAANLHLAAVGPVVDAGIWIMNSKLMDVELIRDAILGSVKHTFY 120
           DTSALFGSLSTLELLRAAANLHLAAVGPVVDAGIWIMNSKLMDVELIR+AILGSVKHTFY
Sbjct: 61  DTSALFGSLSTLELLRAAANLHLAAVGPVVDAGIWIMNSKLMDVELIREAILGSVKHTFY 120

Query: 121 RHFCAGEDDPAVRKTVRRLHDVGLRSMLDYALEYADDEESCDRNLDGFLKTIEATKSLPP 180
           RHFCAGEDD AVRKTVRRLHDVGLRSMLDYALEYADDEESCDRNLDGFLKTIEA KSLPP
Sbjct: 121 RHFCAGEDDAAVRKTVRRLHDVGLRSMLDYALEYADDEESCDRNLDGFLKTIEAIKSLPP 180

Query: 181 GSASFVVAKVSAICPLRLLERVSDLLRWQHKNPCLNLPWKLKTLPIFSDSSPLYHTLKRP 240
           GSASFVVAKVSAICPLRLLERVSDLLRWQHKNPCLNLPWK KTLPIFSDSSPLYHTLK P
Sbjct: 181 GSASFVVAKVSAICPLRLLERVSDLLRWQHKNPCLNLPWKFKTLPIFSDSSPLYHTLKHP 240

Query: 241 PPLTPEEEKSLQISHQRLIKICQACADANVPLAIDAEHTKVQPAIDYFTYSAAIDYNKDH 300
            PLTPEEEKSLQISHQRL+KICQA ADANVPLAIDAEHTKVQPAIDYFTYSAAIDYNKDH
Sbjct: 241 TPLTPEEEKSLQISHQRLMKICQASADANVPLAIDAEHTKVQPAIDYFTYSAAIDYNKDH 300

Query: 301 NPIVYGTVQAYLKDAKDRLLLVTKAADNMKIPLGIKLVRGAYMSTESKLASSLGFPSPIH 360
           NPIVYGTVQAYLKDAKDRLLLVTKAADNMKIPLGIKLVRGAYMS+ESKLA SLGFPSPIH
Sbjct: 301 NPIVYGTVQAYLKDAKDRLLLVTKAADNMKIPLGIKLVRGAYMSSESKLALSLGFPSPIH 360

Query: 361 DTILDTHSCYDGCASFLLDNVVKSSSGVILATHNVESGKLAATKAYEIGIGKLKHKLEFA 420
           DTILDTHSCY+GCASFLLDNVVKSSSGVILATHNVESGKLAA KAYEIGIGKLKHKLEFA
Sbjct: 361 DTILDTHSCYNGCASFLLDNVVKSSSGVILATHNVESGKLAAAKAYEIGIGKLKHKLEFA 420

Query: 421 QLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRKLM 478
           QLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRKLM
Sbjct: 421 QLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRKLM 475

BLAST of Carg05912 vs. ExPASy TrEMBL
Match: A0A6J1FGN7 (Proline dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111445322 PE=3 SV=1)

HSP 1 Score: 778.1 bits (2008), Expect = 2.1e-221
Identity = 392/478 (82.01%), Postives = 433/478 (90.59%), Query Frame = 0

Query: 1   MARRILATQSKTPKRFFIYTFDRLLNTAASSPAAAFTAAAASASP-LNHTGSPATPTIDF 60
           MAR IL+TQSK PK  F Y F+RLLNTAASS AAAFT+AA++  P LN   SPA PT+DF
Sbjct: 1   MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSAASTPPPHLNLAVSPAAPTVDF 60

Query: 61  TDTSALFGSLSTLELLRAAANLHLAAVGPVVDAGIWIMNSKLMDVELIRDAILGSVKHTF 120
           +DT ALFGSLSTLE+L AAA+LH+AAVGPVVD G+W+MNSKLMDVEL RD I G+V+H+F
Sbjct: 61  SDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRHSF 120

Query: 121 YRHFCAGEDDPAVRKTVRRLHDVGLRSMLDYALEYADDEESCDRNLDGFLKTIEATKSLP 180
           YRHFCAGEDD AV KTVRRLH+VGLRSMLDYA+EYA+DEESCDRNL GFL+TIEAT+ LP
Sbjct: 121 YRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHGFLRTIEATRVLP 180

Query: 181 PGSASFVVAKVSAICPLRLLERVSDLLRWQHKNPCLNLPWKLKTLPIFSDSSPLYHTLKR 240
            GSASFVVAKVSAICPLRLLERVSDLLRWQ K+PCLNLPWKLKT PIFS+SSPLYHTL++
Sbjct: 181 RGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPIFSESSPLYHTLQQ 240

Query: 241 PPPLTPEEEKSLQISHQRLIKICQACADANVPLAIDAEHTKVQPAIDYFTYSAAIDYNKD 300
           P PLT EE+KSLQISHQRL+KICQ+C DANVPLAIDAEHTK+QPAIDYFTYSAAI +NKD
Sbjct: 241 PNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHNKD 300

Query: 301 HNPIVYGTVQAYLKDAKDRLLLVTKAADNMKIPLGIKLVRGAYMSTESKLASSLGFPSPI 360
           HNPIVYGT+QAYLKDAKDRLLLVTK A+ M++PLGIKLVRGAYMS+ESKLASSLGF SPI
Sbjct: 301 HNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGFESPI 360

Query: 361 HDTILDTHSCYDGCASFLLDNVVKSSSGVILATHNVESGKLAATKAYEIGIGKLKHKLEF 420
           H++I DTH+CY+ CASFLLDN+   SSG ILATHNVESGKLAA KAYEIGI KLK KLEF
Sbjct: 361 HNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKKKLEF 420

Query: 421 AQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRKLM 478
           AQLYGMSEALS+GLRNAGFQVSKYMPFG VDMVMPYLLRRAEENRGLLS SNLDR+LM
Sbjct: 421 AQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLM 478

BLAST of Carg05912 vs. ExPASy TrEMBL
Match: A0A0A0KXN4 (Proline dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_5G643300 PE=3 SV=1)

HSP 1 Score: 777.3 bits (2006), Expect = 3.6e-221
Identity = 389/479 (81.21%), Postives = 427/479 (89.14%), Query Frame = 0

Query: 1   MARRILATQSKTPKRFFIYTFDRLLNTAASSPAAAFTAAAASASPLN--HTGSPATPTID 60
           MA+ I++TQSK  KR F Y F+R LNTAASSPAAAFTAA A+ SP +  H   P+TPT+D
Sbjct: 1   MAQAIVSTQSKISKRVFFYVFNRPLNTAASSPAAAFTAATATTSPPSPLHLNPPSTPTVD 60

Query: 61  FTDTSALFGSLSTLELLRAAANLHLAAVGPVVDAGIWIMNSKLMDVELIRDAILGSVKHT 120
           FTD+ ALFGS+ T +LL A A LH AAVGPVVD G+W+MNSKLMDVEL RD +LG+VKH+
Sbjct: 61  FTDSRALFGSIPTSDLLHATATLHAAAVGPVVDVGMWVMNSKLMDVELFRDVVLGTVKHS 120

Query: 121 FYRHFCAGEDDPAVRKTVRRLHDVGLRSMLDYALEYADDEESCDRNLDGFLKTIEATKSL 180
           FYRHFCAGEDD +V KTVRRLHDVGLRSMLDYALEYADDE SCDRNLDGFL T+EATKSL
Sbjct: 121 FYRHFCAGEDDTSVAKTVRRLHDVGLRSMLDYALEYADDEASCDRNLDGFLSTVEATKSL 180

Query: 181 PPGSASFVVAKVSAICPLRLLERVSDLLRWQHKNPCLNLPWKLKTLPIFSDSSPLYHTLK 240
           P GSASFVV KVSAICPLRLLERVSDLLRWQ KNP  NLPWKL+TLPIFS+SSPLYHTL+
Sbjct: 181 PSGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLE 240

Query: 241 RPPPLTPEEEKSLQISHQRLIKICQACADANVPLAIDAEHTKVQPAIDYFTYSAAIDYNK 300
           +P PLT EEEKSLQ+SH+RL+KICQ+C DANVPLAIDAEHTKVQPAIDYFTYSAAI +NK
Sbjct: 241 QPEPLTREEEKSLQMSHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNK 300

Query: 301 DHNPIVYGTVQAYLKDAKDRLLLVTKAADNMKIPLGIKLVRGAYMSTESKLASSLGFPSP 360
           D NPIVYGT+QAYLKDAKDRLLL  K A  +K+PLGIKLVRGAYMS+ESKLASSLGF SP
Sbjct: 301 DRNPIVYGTIQAYLKDAKDRLLLANKEASKLKVPLGIKLVRGAYMSSESKLASSLGFESP 360

Query: 361 IHDTILDTHSCYDGCASFLLDNVVKSSSGVILATHNVESGKLAATKAYEIGIGKLKHKLE 420
           IHDTI DTHSCY+ CASFLLD++ K S+G ILATHNVESGKLAA++AYEIGIGKLK KLE
Sbjct: 361 IHDTIQDTHSCYNTCASFLLDDIAKGSTGAILATHNVESGKLAASRAYEIGIGKLKQKLE 420

Query: 421 FAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRKLM 478
           FAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLS SNLDR+LM
Sbjct: 421 FAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRELM 479

BLAST of Carg05912 vs. ExPASy TrEMBL
Match: A0A1S3CBE9 (Proline dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103498577 PE=3 SV=1)

HSP 1 Score: 775.4 bits (2001), Expect = 1.4e-220
Identity = 388/477 (81.34%), Postives = 424/477 (88.89%), Query Frame = 0

Query: 1   MARRILATQSKTPKRFFIYTFDRLLNTAASSPAAAFTAAAASASPLNHTGSPATPTIDFT 60
           MAR +++TQSK  KR F+Y F+R LNTAASSPAAAFTA      P  H   P+TPT+DFT
Sbjct: 1   MARALVSTQSKISKRVFLYVFNRPLNTAASSPAAAFTA----PPPPLHLNPPSTPTVDFT 60

Query: 61  DTSALFGSLSTLELLRAAANLHLAAVGPVVDAGIWIMNSKLMDVELIRDAILGSVKHTFY 120
           D+ ALFGS+ T +LL A A LH +A+GPVVD G+W+MNSKLMDVEL RD +LG+VKHTFY
Sbjct: 61  DSRALFGSIPTSDLLHATATLHASAIGPVVDVGMWVMNSKLMDVELFRDVVLGTVKHTFY 120

Query: 121 RHFCAGEDDPAVRKTVRRLHDVGLRSMLDYALEYADDEESCDRNLDGFLKTIEATKSLPP 180
           RHFCAGEDD +V KTVRRLHDVGLRSMLDYALEYADDE SCDRNLDGFL+T+EATKSLP 
Sbjct: 121 RHFCAGEDDTSVAKTVRRLHDVGLRSMLDYALEYADDEASCDRNLDGFLRTVEATKSLPS 180

Query: 181 GSASFVVAKVSAICPLRLLERVSDLLRWQHKNPCLNLPWKLKTLPIFSDSSPLYHTLKRP 240
           GSASFVV KVSAICPLRLLERVSDLLRWQ KNP  NLPWKL+TLPIFS+SSPLYHTL++P
Sbjct: 181 GSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLEQP 240

Query: 241 PPLTPEEEKSLQISHQRLIKICQACADANVPLAIDAEHTKVQPAIDYFTYSAAIDYNKDH 300
            PLT EEEKSLQISH+RL+KICQ+C DANVPLAIDAEHTKVQPAIDYFTYSAAI +NKD 
Sbjct: 241 EPLTREEEKSLQISHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNKDR 300

Query: 301 NPIVYGTVQAYLKDAKDRLLLVTKAADNMKIPLGIKLVRGAYMSTESKLASSLGFPSPIH 360
           NPIVYGT+QAYLKDAKDRLLL  K A  +KIPLGIKLVRGAYMS+ESKLASSLGF SPIH
Sbjct: 301 NPIVYGTIQAYLKDAKDRLLLANKEASKLKIPLGIKLVRGAYMSSESKLASSLGFESPIH 360

Query: 361 DTILDTHSCYDGCASFLLDNVVKSSSGVILATHNVESGKLAATKAYEIGIGKLKHKLEFA 420
           +TI DTHSCY+ CASFLLD++ K SSG ILATHNVESGKLAAT+AYEIGIGKLK KLEFA
Sbjct: 361 NTIQDTHSCYNTCASFLLDDIAKGSSGAILATHNVESGKLAATRAYEIGIGKLKQKLEFA 420

Query: 421 QLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRKLM 478
           QLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLS SNLDR+LM
Sbjct: 421 QLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRQLM 473

BLAST of Carg05912 vs. TAIR 10
Match: AT3G30775.1 (Methylenetetrahydrofolate reductase family protein )

HSP 1 Score: 496.9 bits (1278), Expect = 1.8e-140
Identity = 244/424 (57.55%), Postives = 316/424 (74.53%), Query Frame = 0

Query: 57  IDFTDTSALFGSLSTLELLRAAANLHLAAVGPVVDAGIWIMNSKLMDVELIRDAILGSVK 116
           +D +D + LF S+ T +LLR+ A LH AA+GP+VD G W+M+SKLMD  + R  +LG VK
Sbjct: 63  LDLSDQARLFSSIPTSDLLRSTAVLHAAAIGPMVDLGTWVMSSKLMDASVTRGMVLGLVK 122

Query: 117 HTFYRHFCAGEDDPAVRKTVRRLHD-VGLRSMLDYALEYADDEESCDRNLDGFLKTIEAT 176
            TFY HFCAGED  A  + VR +++  GL+ ML Y +E+ADD  SCD N+  F++TIEA 
Sbjct: 123 STFYDHFCAGEDADAAAERVRSVYEATGLKGMLVYGVEHADDAVSCDDNMQQFIRTIEAA 182

Query: 177 KSLPPGSASFVVAKVSAICPLRLLERVSDLLRWQHKNPCLNLPWKLKTLPIFSDSSPLYH 236
           KSLP    S VV K++AICP+ LL+RVSDLLRW++K+P   L WKLK+ P+FS+SSPLYH
Sbjct: 183 KSLPTSHFSSVVVKITAICPISLLKRVSDLLRWEYKSPNFKLSWKLKSFPVFSESSPLYH 242

Query: 237 TLKRPPPLTPEEEKSLQISHQRLIKICQACADANVPLAIDAEHTKVQPAIDYFTYSAAID 296
           T   P PLT EEE+ L+ +H R+ +IC+ C ++NVPL IDAE T +QPAIDY  YS+AI 
Sbjct: 243 TNSEPEPLTAEEERELEAAHGRIQEICRKCQESNVPLLIDAEDTILQPAIDYMAYSSAIM 302

Query: 297 YNKDHN-PIVYGTVQAYLKDAKDRLLLVTKAADNMKIPLGIKLVRGAYMSTESKLASSLG 356
           +N D + PIVY T+QAYL+DA +RL L  + A+   +P+G KLVRGAYMS+E+ LA SLG
Sbjct: 303 FNADKDRPIVYNTIQAYLRDAGERLHLAVQNAEKENVPMGFKLVRGAYMSSEASLADSLG 362

Query: 357 FPSPIHDTILDTHSCYDGCASFLLDNVVKSSS-GVILATHNVESGKLAATKAYEIGIGKL 416
             SP+HDTI DTHSCY+ C +FL++     S  GV+LATHN +SG+LA+ KA ++GI K 
Sbjct: 363 CKSPVHDTIQDTHSCYNDCMTFLMEKASNGSGFGVVLATHNADSGRLASRKASDLGIDKQ 422

Query: 417 KHKLEFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLD 476
             K+EFAQLYGMS+ALSFGL+ AGF VSKYMPFGPV   +PYLLRRA ENRG+++T   D
Sbjct: 423 NGKIEFAQLYGMSDALSFGLKRAGFNVSKYMPFGPVATAIPYLLRRAYENRGMMATGAHD 482

Query: 477 RKLM 478
           R+LM
Sbjct: 483 RQLM 486

BLAST of Carg05912 vs. TAIR 10
Match: AT5G38710.1 (Methylenetetrahydrofolate reductase family protein )

HSP 1 Score: 490.0 bits (1260), Expect = 2.2e-138
Identity = 239/424 (56.37%), Postives = 310/424 (73.11%), Query Frame = 0

Query: 57  IDFTDTSALFGSLSTLELLRAAANLHLAAVGPVVDAGIWIMNSKLMDVELIRDAILGSVK 116
           +D +D + LF S+    LLR+ A LH  ++GP+VD G W+M+SKLMD  + RD +L  VK
Sbjct: 42  LDLSDQARLFASVPISTLLRSTAILHATSIGPMVDLGSWLMSSKLMDTTVTRDLVLRIVK 101

Query: 117 HTFYRHFCAGEDDPAVRKTVRRLHD-VGLRSMLDYALEYADDEESCDRNLDGFLKTIEAT 176
            TFY HFCAGED  A  + V  +++  GL+ ML Y +E+A+D  +CD N+  F++T+EA 
Sbjct: 102 GTFYDHFCAGEDAAAAARRVSSVYESTGLKGMLVYGVEHAEDGGACDENIQKFIETVEAA 161

Query: 177 KSLPPGSASFVVAKVSAICPLRLLERVSDLLRWQHKNPCLNLPWKLKTLPIFSDSSPLYH 236
           K+LP    S VV K++AICP+ +L+RVSDLLRWQ+KNP   LPWKL + P+FS  SPLYH
Sbjct: 162 KTLPSSHLSSVVVKITAICPMNVLKRVSDLLRWQYKNPNFKLPWKLNSFPVFSGLSPLYH 221

Query: 237 TLKRPPPLTPEEEKSLQISHQRLIKICQACADANVPLAIDAEHTKVQPAIDYFTYSAAID 296
           T   P PLT EEE+ L+ +H+RL  +C  C ++NVPL IDAE T +QPAIDY  Y +AI 
Sbjct: 222 TTSEPEPLTVEEERELEKAHERLKSVCLRCQESNVPLLIDAEDTILQPAIDYMAYWSAIM 281

Query: 297 YNKDHN-PIVYGTVQAYLKDAKDRLLLVTKAADNMKIPLGIKLVRGAYMSTESKLASSLG 356
           +N D + PIVY T+QAYLKDA +RL L  + ++ M +P+G KLVRGAYMS+E+KLA SLG
Sbjct: 282 FNSDKDRPIVYNTIQAYLKDAGERLHLALRESEKMNVPIGFKLVRGAYMSSEAKLADSLG 341

Query: 357 FPSPIHDTILDTHSCYDGCASFLLDNVVKSSS-GVILATHNVESGKLAATKAYEIGIGKL 416
           + SP+HDTI +TH CY+ C SFL++     S   VILATHN +SGKL A KA E+GI K 
Sbjct: 342 YKSPVHDTIQNTHDCYNDCMSFLMEKASNGSGIAVILATHNTDSGKLGARKASELGINKE 401

Query: 417 KHKLEFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLD 476
             K+EFAQLYGMS+ALSFGL+ AGF VSKYMP+GPVD  +PYL+RRA ENRG++ST  LD
Sbjct: 402 NGKIEFAQLYGMSDALSFGLKRAGFNVSKYMPYGPVDTAIPYLIRRAYENRGMMSTGALD 461

Query: 477 RKLM 478
           R+LM
Sbjct: 462 RQLM 465