Carg05886 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg05886
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionMethyl esterase
LocationCarg_Chr04: 20929615 .. 20932440 (+)
RNA-Seq ExpressionCarg05886
SyntenyCarg05886
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTAATTTATGTGCCTGTCTCGCACCCAAATCAATGAAGAAAAAACCCACCAAGCGCCTTCACAACCCACCTCCGACCACCAATTCTACCAAAAGATGGACCCGAGTTCGTTCTTCCAGGAAAGAAAAATCCGATGCTGTTCTGACTCAAGAGCAAGTTCTCGCCGCCGCGATTCTGTTCCAACAACAGAATGGCCGCGACCCTTTTGATCGGACCACTTCTGTCCGGTACCCGAAATCAGGGTCGAAGAATAGCAACACTCTGCCGAGGAGCTCTAGTTCGCGGGCTCGGGCTCTTACCGATCCCCTGCTTCAGCCTCATCAGCTTGTTAGCGAGGTTGTGATTAAACCCCCTTTTTTGAACTGTTTTGCTTCTTTTTTAGTTCACGAATTGATGGAGTCTCTGCATTTCGGAATGGGTTGCTTGATTTCATTTGTTTTGACGCTTTCTCCTCTGTTCATGCAGAATGTAAAACTTGAGGATATAGAAACGAATCATTTTGTTCTTGTCCATGGTGGTGGCTTTGGTGCTTGGTGTTGGTATAAAACCATAGCACTTCTAGAAGAAGGGGGTTACAAAGCTACTGCCATAGATTTAGCTGGTTCTGGAGTTCATTCATTTGATCCTAATCGCATCACAAGTCTTTTGCAATATGTGCACCCGCTTACTGATTTCCTTCAAATGCTTCCTGAGGGAGAAAAGGTCTTTGAACTCATCCATTAATTTCTTTTTCAGTTCTGTTTAATGTTTCTGATGTTGTTCCTTAATCAAGTTAGAGCTTAGAGGTAAGAATCTAAAACTCGTTATCGTCCCTGCCACAACTAGCATATATTGTCCTCTTTGGCTTTTCCTTTCGAGTTTCTCCTCAAAGTTTTTAAAACGCTCTGTTAGGGAGATGTTTCTATACTTCTATAAAAAGATTTCGTAATCCTCCCCAACCGATGTGATATCTCACAATCCACCCCGTTCGAGGCCTAGTGTCTTTTTGGCATTTGTTCCCTTATTCAATCGACGTGGGATTCCCCAATCCACCCTCTTTAGAGTCCAACGTTCTTGTTGGCACACCTCCTTGTGTCTACCTTTTTTGGGCTCATCCTCCTTGCTGGCACATCGCTTGGTATATGTAATATCTTAAGTCCACCGCCGGTAGATATTGTTCTATGTGGACTTTCCCGTCTGGGTTTCCTGTTCGGGTTACAAGTGCCATATACTAAAAAGGCTAGGTTTACTGAAAAAGTTTTTGTTTCCTTCCTCATATTTGGAACTTCCTTACTCTATTCATGCAGGTTATCTTGGTGGGACATGATTTTGGCGGTGCATGTATAGCATATGCAATGGAGTTATTTCCTTTTAGAATTTCAAAAGCCATATTCATCGCTGCAGCAATGTTGAGTAATGGACAGAACACTTTAGACATGTTTTCTCAGCAGGTATGCATTGTTAATCTGTGATTCATAGATACGTATCTATTCTGTTTAAGTATGTGAATTGTGTTGAGGATTGTTGGGAGAGGAATTCCACATCGGCTAATCGGCTAATTAAGGGGTTGATCATGAGTTTATAAGTAAGAAATACATCTTCATTGGTATGAGGCCTTTTCGGGAAACACAAAACAAAGCCATAAGAGCTTATGCGCAAAGTGGACAATATCATACCATTGTAGAGATTCGTGATTCCTAACAAGCTATTAGAGTCCTGCCCTTAACTTAGTCATGTCAATAGAATCCTTAAATATCGAACAAAGAAGTTGTGAGCCTCGAAGATGTAGTCAAAAGTGTCGAACAAAATGTGACTTTGTTCGACGACTCCATAAGCCTTAAGAGAGGGCTCTATGTTGTACTTTGTTTGGGAGGGGGGAGGATTGTTGAGAGAGGAGTCCCACTTCGACTAATTAAGGGACCATGCTCAAAGTGGACAATATCATACCAATGTGGAGATTCGTGGTTCTTAACAAATGGTGTCTTGTTTGCCTACTGCAGGCTGGTGCAGATGATCTAATGCGGCAGGCTCAGACATTTTCATATGCAAATGGGAACGATCGTCCTCCAACCTCTATTAACTTCGAGAAATCGTTGTTGAGGGACTTGTTTTTCAATCAATGTCCTGCCAAGGTAATGGGGTTGAGAAACATTGAATATGCATACTTTTTTCCCCATAAGCTAAACGACATGTTTGGGAGTGATTTTGGTCGTGTTGAACATTAACCGTTGCTCGTGTTCTTTTGCCTCTTTCGTTTCATACAAGCTCATGGCCGTTTGTATTCATGGTCCAGGATGTGGCTCTGGCGTCTGTATCAATGAGACCGATGCCATTTGCGCCTATTTTGGAGAAGCTCTGCCTTACAGACTTAAACTATGGATTGGTCAAGCGGTTTTACATAGAAACTCCAGAAGACAATGCCATTCCCATCACATTACAGGACAGCATGATAAACAAAAGCCCCCCACAACAGGTCTTTCGGCTAAAAGGTGCAGATCATTCCCCTTTCTTCTCAAAGCCTCAAGCTCTAAACAAGCTATTGGTAGAGATCTCAAAAATCAAAACACCATCATAGCATCACTCGACTCGGAAACGACTCAGAAATCTGTGAAAATCGTCGCCCGGATCGTAAGGTCAGAACATATGAATAATGTATAGGCTCATTAAACAGCAAGAACAGTAAAAGTTCTGGTTACTTGATTAAAAAATGAATCATGCTTCCTTTTACATTTGTATGCTCTTTCTTTGTTTCTGTTTCTGAGAGGATTTAGGGGATTTTTGTTTGCTTTCCCCTTCGTCCCTAGGAATTTTTGCAGCAACAAATATATTGTTTTAAGGATAAAC

mRNA sequence

ATGGGTAATTTATGTGCCTGTCTCGCACCCAAATCAATGAAGAAAAAACCCACCAAGCGCCTTCACAACCCACCTCCGACCACCAATTCTACCAAAAGATGGACCCGAGTTCGTTCTTCCAGGAAAGAAAAATCCGATGCTGTTCTGACTCAAGAGCAAGTTCTCGCCGCCGCGATTCTGTTCCAACAACAGAATGGCCGCGACCCTTTTGATCGGACCACTTCTGTCCGGTACCCGAAATCAGGGTCGAAGAATAGCAACACTCTGCCGAGGAGCTCTAGTTCGCGGGCTCGGGCTCTTACCGATCCCCTGCTTCAGCCTCATCAGCTTGTTAGCGAGAATGTAAAACTTGAGGATATAGAAACGAATCATTTTGTTCTTGTCCATGGTGGTGGCTTTGGTGCTTGGTGTTGGTATAAAACCATAGCACTTCTAGAAGAAGGGGGTTACAAAGCTACTGCCATAGATTTAGCTGGTTCTGGAGTTCATTCATTTGATCCTAATCGCATCACAAGTCTTTTGCAATATGTGCACCCGCTTACTGATTTCCTTCAAATGCTTCCTGAGGGAGAAAAGAGTCCAACGTTCTTGTTGGCACACCTCCTTGTGTCTACCTTTTTTGGGCTCATCCTCCTTGCTGGCACATCGCTTGGTATATGTAATATCTTAAGTCCACCGCCGGTAGATATTGTTCTATGTGGACTTTCCCGTCTGGGTTTCCTGTTCGGGTTACAAGTTATCTTGGTGGGACATGATTTTGGCGGTGCATGTATAGCATATGCAATGGAGTTATTTCCTTTTAGAATTTCAAAAGCCATATTCATCGCTGCAGCAATGTTGAGTAATGGACAGAACACTTTAGACATGTTTTCTCAGCAGGCTGGTGCAGATGATCTAATGCGGCAGGCTCAGACATTTTCATATGCAAATGGGAACGATCGTCCTCCAACCTCTATTAACTTCGAGAAATCGTTGTTGAGGGACTTGTTTTTCAATCAATGTCCTGCCAAGGATGTGGCTCTGGCGTCTGTATCAATGAGACCGATGCCATTTGCGCCTATTTTGGAGAAGCTCTGCCTTACAGACTTAAACTATGGATTGGTCAAGCGGTTTTACATAGAAACTCCAGAAGACAATGCCATTCCCATCACATTACAGGACAGCATGATAAACAAAAGCCCCCCACAACAGGTCTTTCGGCTAAAAGGTGCAGATCATTCCCCTTTCTTCTCAAAGCCTCAAGCTCTAAACAAGCTATTGGTAGAGATCTCAAAAATCAAAACACCATCATAGCATCACTCGACTCGGAAACGACTCAGAAATCTGTGAAAATCGTCGCCCGGATCGTAAGGTCAGAACATATGAATAATGTATAGGCTCATTAAACAGCAAGAACAGTAAAAGTTCTGGTTACTTGATTAAAAAATGAATCATGCTTCCTTTTACATTTGTATGCTCTTTCTTTGTTTCTGTTTCTGAGAGGATTTAGGGGATTTTTGTTTGCTTTCCCCTTCGTCCCTAGGAATTTTTGCAGCAACAAATATATTGTTTTAAGGATAAAC

Coding sequence (CDS)

ATGGGTAATTTATGTGCCTGTCTCGCACCCAAATCAATGAAGAAAAAACCCACCAAGCGCCTTCACAACCCACCTCCGACCACCAATTCTACCAAAAGATGGACCCGAGTTCGTTCTTCCAGGAAAGAAAAATCCGATGCTGTTCTGACTCAAGAGCAAGTTCTCGCCGCCGCGATTCTGTTCCAACAACAGAATGGCCGCGACCCTTTTGATCGGACCACTTCTGTCCGGTACCCGAAATCAGGGTCGAAGAATAGCAACACTCTGCCGAGGAGCTCTAGTTCGCGGGCTCGGGCTCTTACCGATCCCCTGCTTCAGCCTCATCAGCTTGTTAGCGAGAATGTAAAACTTGAGGATATAGAAACGAATCATTTTGTTCTTGTCCATGGTGGTGGCTTTGGTGCTTGGTGTTGGTATAAAACCATAGCACTTCTAGAAGAAGGGGGTTACAAAGCTACTGCCATAGATTTAGCTGGTTCTGGAGTTCATTCATTTGATCCTAATCGCATCACAAGTCTTTTGCAATATGTGCACCCGCTTACTGATTTCCTTCAAATGCTTCCTGAGGGAGAAAAGAGTCCAACGTTCTTGTTGGCACACCTCCTTGTGTCTACCTTTTTTGGGCTCATCCTCCTTGCTGGCACATCGCTTGGTATATGTAATATCTTAAGTCCACCGCCGGTAGATATTGTTCTATGTGGACTTTCCCGTCTGGGTTTCCTGTTCGGGTTACAAGTTATCTTGGTGGGACATGATTTTGGCGGTGCATGTATAGCATATGCAATGGAGTTATTTCCTTTTAGAATTTCAAAAGCCATATTCATCGCTGCAGCAATGTTGAGTAATGGACAGAACACTTTAGACATGTTTTCTCAGCAGGCTGGTGCAGATGATCTAATGCGGCAGGCTCAGACATTTTCATATGCAAATGGGAACGATCGTCCTCCAACCTCTATTAACTTCGAGAAATCGTTGTTGAGGGACTTGTTTTTCAATCAATGTCCTGCCAAGGATGTGGCTCTGGCGTCTGTATCAATGAGACCGATGCCATTTGCGCCTATTTTGGAGAAGCTCTGCCTTACAGACTTAAACTATGGATTGGTCAAGCGGTTTTACATAGAAACTCCAGAAGACAATGCCATTCCCATCACATTACAGGACAGCATGATAAACAAAAGCCCCCCACAACAGGTCTTTCGGCTAAAAGGTGCAGATCATTCCCCTTTCTTCTCAAAGCCTCAAGCTCTAAACAAGCTATTGGTAGAGATCTCAAAAATCAAAACACCATCATAG

Protein sequence

MGNLCACLAPKSMKKKPTKRLHNPPPTTNSTKRWTRVRSSRKEKSDAVLTQEQVLAAAILFQQQNGRDPFDRTTSVRYPKSGSKNSNTLPRSSSSRARALTDPLLQPHQLVSENVKLEDIETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLAGSGVHSFDPNRITSLLQYVHPLTDFLQMLPEGEKSPTFLLAHLLVSTFFGLILLAGTSLGICNILSPPPVDIVLCGLSRLGFLFGLQVILVGHDFGGACIAYAMELFPFRISKAIFIAAAMLSNGQNTLDMFSQQAGADDLMRQAQTFSYANGNDRPPTSINFEKSLLRDLFFNQCPAKDVALASVSMRPMPFAPILEKLCLTDLNYGLVKRFYIETPEDNAIPITLQDSMINKSPPQQVFRLKGADHSPFFSKPQALNKLLVEISKIKTPS
Homology
BLAST of Carg05886 vs. NCBI nr
Match: KAG7033209.1 (putative methylesterase 11, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 855.9 bits (2210), Expect = 1.5e-244
Identity = 430/430 (100.00%), Postives = 430/430 (100.00%), Query Frame = 0

Query: 1   MGNLCACLAPKSMKKKPTKRLHNPPPTTNSTKRWTRVRSSRKEKSDAVLTQEQVLAAAIL 60
           MGNLCACLAPKSMKKKPTKRLHNPPPTTNSTKRWTRVRSSRKEKSDAVLTQEQVLAAAIL
Sbjct: 1   MGNLCACLAPKSMKKKPTKRLHNPPPTTNSTKRWTRVRSSRKEKSDAVLTQEQVLAAAIL 60

Query: 61  FQQQNGRDPFDRTTSVRYPKSGSKNSNTLPRSSSSRARALTDPLLQPHQLVSENVKLEDI 120
           FQQQNGRDPFDRTTSVRYPKSGSKNSNTLPRSSSSRARALTDPLLQPHQLVSENVKLEDI
Sbjct: 61  FQQQNGRDPFDRTTSVRYPKSGSKNSNTLPRSSSSRARALTDPLLQPHQLVSENVKLEDI 120

Query: 121 ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLAGSGVHSFDPNRITSLLQYVHPL 180
           ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLAGSGVHSFDPNRITSLLQYVHPL
Sbjct: 121 ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLAGSGVHSFDPNRITSLLQYVHPL 180

Query: 181 TDFLQMLPEGEKSPTFLLAHLLVSTFFGLILLAGTSLGICNILSPPPVDIVLCGLSRLGF 240
           TDFLQMLPEGEKSPTFLLAHLLVSTFFGLILLAGTSLGICNILSPPPVDIVLCGLSRLGF
Sbjct: 181 TDFLQMLPEGEKSPTFLLAHLLVSTFFGLILLAGTSLGICNILSPPPVDIVLCGLSRLGF 240

Query: 241 LFGLQVILVGHDFGGACIAYAMELFPFRISKAIFIAAAMLSNGQNTLDMFSQQAGADDLM 300
           LFGLQVILVGHDFGGACIAYAMELFPFRISKAIFIAAAMLSNGQNTLDMFSQQAGADDLM
Sbjct: 241 LFGLQVILVGHDFGGACIAYAMELFPFRISKAIFIAAAMLSNGQNTLDMFSQQAGADDLM 300

Query: 301 RQAQTFSYANGNDRPPTSINFEKSLLRDLFFNQCPAKDVALASVSMRPMPFAPILEKLCL 360
           RQAQTFSYANGNDRPPTSINFEKSLLRDLFFNQCPAKDVALASVSMRPMPFAPILEKLCL
Sbjct: 301 RQAQTFSYANGNDRPPTSINFEKSLLRDLFFNQCPAKDVALASVSMRPMPFAPILEKLCL 360

Query: 361 TDLNYGLVKRFYIETPEDNAIPITLQDSMINKSPPQQVFRLKGADHSPFFSKPQALNKLL 420
           TDLNYGLVKRFYIETPEDNAIPITLQDSMINKSPPQQVFRLKGADHSPFFSKPQALNKLL
Sbjct: 361 TDLNYGLVKRFYIETPEDNAIPITLQDSMINKSPPQQVFRLKGADHSPFFSKPQALNKLL 420

Query: 421 VEISKIKTPS 431
           VEISKIKTPS
Sbjct: 421 VEISKIKTPS 430

BLAST of Carg05886 vs. NCBI nr
Match: XP_023538126.1 (putative methylesterase 11, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 729.9 bits (1883), Expect = 1.2e-206
Identity = 377/430 (87.67%), Postives = 377/430 (87.67%), Query Frame = 0

Query: 1   MGNLCACLAPKSMKKKPTKRLHNPPPTTNSTKRWTRVRSSRKEKSDAVLTQEQVLAAAIL 60
           MGNLCACLAPKSMKKKPTKRLHNPPPTTNSTKRWTRVRSSRKEKSDAVLTQEQVLAAAIL
Sbjct: 1   MGNLCACLAPKSMKKKPTKRLHNPPPTTNSTKRWTRVRSSRKEKSDAVLTQEQVLAAAIL 60

Query: 61  FQQQNGRDPFDRTTSVRYPKSGSKNSNTLPRSSSSRARALTDPLLQPHQLVSENVKLEDI 120
           FQQQNGRDPFDRTTSVRYPKSGSKNSNTLPRSSSSRARALTDPLLQPHQLVSENVKLEDI
Sbjct: 61  FQQQNGRDPFDRTTSVRYPKSGSKNSNTLPRSSSSRARALTDPLLQPHQLVSENVKLEDI 120

Query: 121 ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLAGSGVHSFDPNRITSLLQYVHPL 180
           ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLAGSGVHSFDPNRITSLLQYVHPL
Sbjct: 121 ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLAGSGVHSFDPNRITSLLQYVHPL 180

Query: 181 TDFLQMLPEGEKSPTFLLAHLLVSTFFGLILLAGTSLGICNILSPPPVDIVLCGLSRLGF 240
           TDFLQMLPEGEK                                                
Sbjct: 181 TDFLQMLPEGEK------------------------------------------------ 240

Query: 241 LFGLQVILVGHDFGGACIAYAMELFPFRISKAIFIAAAMLSNGQNTLDMFSQQAGADDLM 300
                VILVGHDFGGACIAYAMELFPFRISKAIFIAAAMLSNGQNTLDMFSQQAGADDLM
Sbjct: 241 -----VILVGHDFGGACIAYAMELFPFRISKAIFIAAAMLSNGQNTLDMFSQQAGADDLM 300

Query: 301 RQAQTFSYANGNDRPPTSINFEKSLLRDLFFNQCPAKDVALASVSMRPMPFAPILEKLCL 360
           RQAQTFSYANGNDRPPTSINFEKSLLRDLFFNQCPAKDVALASVSMRPMPFAPILEKLCL
Sbjct: 301 RQAQTFSYANGNDRPPTSINFEKSLLRDLFFNQCPAKDVALASVSMRPMPFAPILEKLCL 360

Query: 361 TDLNYGLVKRFYIETPEDNAIPITLQDSMINKSPPQQVFRLKGADHSPFFSKPQALNKLL 420
           TDLNYGLVKRFYIETPEDNAIPITLQDSMINKSPPQQVFRLKGADHSPFFSKPQALNKLL
Sbjct: 361 TDLNYGLVKRFYIETPEDNAIPITLQDSMINKSPPQQVFRLKGADHSPFFSKPQALNKLL 377

Query: 421 VEISKIKTPS 431
           VEISKIKTPS
Sbjct: 421 VEISKIKTPS 377

BLAST of Carg05886 vs. NCBI nr
Match: XP_022954463.1 (putative methylesterase 11, chloroplastic [Cucurbita moschata])

HSP 1 Score: 724.5 bits (1869), Expect = 5.2e-205
Identity = 374/430 (86.98%), Postives = 375/430 (87.21%), Query Frame = 0

Query: 1   MGNLCACLAPKSMKKKPTKRLHNPPPTTNSTKRWTRVRSSRKEKSDAVLTQEQVLAAAIL 60
           MGNLCACLAPKSMKKKPTKRLHNPPPTTNSTKRWTRVR SRKEKSDA LTQEQVLAAAIL
Sbjct: 1   MGNLCACLAPKSMKKKPTKRLHNPPPTTNSTKRWTRVRFSRKEKSDAALTQEQVLAAAIL 60

Query: 61  FQQQNGRDPFDRTTSVRYPKSGSKNSNTLPRSSSSRARALTDPLLQPHQLVSENVKLEDI 120
           FQQQNGRDPFDRTTSVRYPKSGSKNSNTLPRSSSSRARALTDPLLQPHQLVSENVKLEDI
Sbjct: 61  FQQQNGRDPFDRTTSVRYPKSGSKNSNTLPRSSSSRARALTDPLLQPHQLVSENVKLEDI 120

Query: 121 ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLAGSGVHSFDPNRITSLLQYVHPL 180
           ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLAGSGVHSFDPNRITSLLQYVHPL
Sbjct: 121 ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLAGSGVHSFDPNRITSLLQYVHPL 180

Query: 181 TDFLQMLPEGEKSPTFLLAHLLVSTFFGLILLAGTSLGICNILSPPPVDIVLCGLSRLGF 240
           TDFLQMLPEGEK                                                
Sbjct: 181 TDFLQMLPEGEK------------------------------------------------ 240

Query: 241 LFGLQVILVGHDFGGACIAYAMELFPFRISKAIFIAAAMLSNGQNTLDMFSQQAGADDLM 300
                VILVGHDFGGACIAYAMELFPFRISKAIFIAAAMLSNGQNTLDMFSQQAGADDLM
Sbjct: 241 -----VILVGHDFGGACIAYAMELFPFRISKAIFIAAAMLSNGQNTLDMFSQQAGADDLM 300

Query: 301 RQAQTFSYANGNDRPPTSINFEKSLLRDLFFNQCPAKDVALASVSMRPMPFAPILEKLCL 360
           R+AQTFSYANGNDRPPTSINFEKSLLRDLFFNQCPAKDVALASVSMRPMPFAPILEKLCL
Sbjct: 301 RRAQTFSYANGNDRPPTSINFEKSLLRDLFFNQCPAKDVALASVSMRPMPFAPILEKLCL 360

Query: 361 TDLNYGLVKRFYIETPEDNAIPITLQDSMINKSPPQQVFRLKGADHSPFFSKPQALNKLL 420
           TDLNYGLVKRFYIETPEDNAIPITLQDSMINKSPPQQVFRLKGADHSPFFSKPQALNKLL
Sbjct: 361 TDLNYGLVKRFYIETPEDNAIPITLQDSMINKSPPQQVFRLKGADHSPFFSKPQALNKLL 377

Query: 421 VEISKIKTPS 431
           VEISKIKTPS
Sbjct: 421 VEISKIKTPS 377

BLAST of Carg05886 vs. NCBI nr
Match: XP_022990993.1 (putative methylesterase 11, chloroplastic [Cucurbita maxima])

HSP 1 Score: 720.7 bits (1859), Expect = 7.5e-204
Identity = 372/430 (86.51%), Postives = 375/430 (87.21%), Query Frame = 0

Query: 1   MGNLCACLAPKSMKKKPTKRLHNPPPTTNSTKRWTRVRSSRKEKSDAVLTQEQVLAAAIL 60
           MGNLCACLAPKSMKKKPTKRL NPPPTTNS+KRWTRVRSSRKEKSDA LTQEQVLAAAIL
Sbjct: 1   MGNLCACLAPKSMKKKPTKRLRNPPPTTNSSKRWTRVRSSRKEKSDAALTQEQVLAAAIL 60

Query: 61  FQQQNGRDPFDRTTSVRYPKSGSKNSNTLPRSSSSRARALTDPLLQPHQLVSENVKLEDI 120
           FQQQNGRDPFDRTTSVRYPKSGSKNSNTLPRSSSSRARALTDPLLQPHQLVSENVKLEDI
Sbjct: 61  FQQQNGRDPFDRTTSVRYPKSGSKNSNTLPRSSSSRARALTDPLLQPHQLVSENVKLEDI 120

Query: 121 ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLAGSGVHSFDPNRITSLLQYVHPL 180
           ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLAGSGVHSFDPNRITSLLQYVHPL
Sbjct: 121 ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLAGSGVHSFDPNRITSLLQYVHPL 180

Query: 181 TDFLQMLPEGEKSPTFLLAHLLVSTFFGLILLAGTSLGICNILSPPPVDIVLCGLSRLGF 240
           TDFLQMLPEGEK                                                
Sbjct: 181 TDFLQMLPEGEK------------------------------------------------ 240

Query: 241 LFGLQVILVGHDFGGACIAYAMELFPFRISKAIFIAAAMLSNGQNTLDMFSQQAGADDLM 300
                VILVGHDFGGACIA+AMELFP+RISKAIFIAAAMLSNGQNTLDMFSQQAGADDLM
Sbjct: 241 -----VILVGHDFGGACIAHAMELFPYRISKAIFIAAAMLSNGQNTLDMFSQQAGADDLM 300

Query: 301 RQAQTFSYANGNDRPPTSINFEKSLLRDLFFNQCPAKDVALASVSMRPMPFAPILEKLCL 360
           RQAQTFSYANGNDRPPTSINFEKSLLRDLFFNQCPAKDVALASVSMRPMPFAPILEKLCL
Sbjct: 301 RQAQTFSYANGNDRPPTSINFEKSLLRDLFFNQCPAKDVALASVSMRPMPFAPILEKLCL 360

Query: 361 TDLNYGLVKRFYIETPEDNAIPITLQDSMINKSPPQQVFRLKGADHSPFFSKPQALNKLL 420
           TDLNYGLVKRFYIETPEDNAIPITLQDSMINKSPPQQVFRLKGADHSPFFSKPQALNKLL
Sbjct: 361 TDLNYGLVKRFYIETPEDNAIPITLQDSMINKSPPQQVFRLKGADHSPFFSKPQALNKLL 377

Query: 421 VEISKIKTPS 431
           VEISKIKTPS
Sbjct: 421 VEISKIKTPS 377

BLAST of Carg05886 vs. NCBI nr
Match: XP_038891023.1 (putative methylesterase 11, chloroplastic [Benincasa hispida])

HSP 1 Score: 650.2 bits (1676), Expect = 1.2e-182
Identity = 338/433 (78.06%), Postives = 357/433 (82.45%), Query Frame = 0

Query: 1   MGNLCACLAPKSMKKKPTKRLHNPPPTTNSTKRWTRVRSSRKEKSDAVLTQEQVLAAAIL 60
           MGN CACLAPKS+KKKPTKRL NPP  TNS+KRWTRVRSSRKEK DA LTQ+QVLAAAIL
Sbjct: 1   MGNFCACLAPKSIKKKPTKRLLNPPLPTNSSKRWTRVRSSRKEKPDAALTQDQVLAAAIL 60

Query: 61  F---QQQNGRDPFDRTTSVRYPKSGSKNSNTLPRSSSSRARALTDPLLQPHQLVSENVKL 120
           F   QQ+NGRDPFDRTTS+RYPKSGSKNSN LPRSSSSRAR+LTDPLLQPHQLVS+NVKL
Sbjct: 61  FQQHQQRNGRDPFDRTTSLRYPKSGSKNSNALPRSSSSRARSLTDPLLQPHQLVSQNVKL 120

Query: 121 EDIETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLAGSGVHSFDPNRITSLLQYV 180
           ED+ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDL GSGVHSFDPNRITSLLQYV
Sbjct: 121 EDVETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLTGSGVHSFDPNRITSLLQYV 180

Query: 181 HPLTDFLQMLPEGEKSPTFLLAHLLVSTFFGLILLAGTSLGICNILSPPPVDIVLCGLSR 240
            PL DFL+ L EGEK                                             
Sbjct: 181 QPLIDFLEKLAEGEK--------------------------------------------- 240

Query: 241 LGFLFGLQVILVGHDFGGACIAYAMELFPFRISKAIFIAAAMLSNGQNTLDMFSQQAGAD 300
                   VILVGHDFGGACIAYAMELFPFRI+KAIFIAAAML+NGQNTLDMFSQQAGAD
Sbjct: 241 --------VILVGHDFGGACIAYAMELFPFRIAKAIFIAAAMLTNGQNTLDMFSQQAGAD 300

Query: 301 DLMRQAQTFSYANGNDRPPTSINFEKSLLRDLFFNQCPAKDVALASVSMRPMPFAPILEK 360
           DLM+QAQTFSY NGN+ PPT+IN EKSLLRDLFFNQ  AKDVALASVSMRP+PFAPILEK
Sbjct: 301 DLMQQAQTFSYTNGNNHPPTAINLEKSLLRDLFFNQSLAKDVALASVSMRPIPFAPILEK 360

Query: 361 LCLTDLNYGLVKRFYIETPEDNAIPITLQDSMINKSPPQQVFRLKGADHSPFFSKPQALN 420
           LCL+DL YG V+RFYIET EDNAIPITLQDSMINKSPP+QVFRLKGADHSPFFSKPQALN
Sbjct: 361 LCLSDLKYGSVRRFYIETTEDNAIPITLQDSMINKSPPEQVFRLKGADHSPFFSKPQALN 380

Query: 421 KLLVEISKIKTPS 431
           KLL+EISKIKTPS
Sbjct: 421 KLLIEISKIKTPS 380

BLAST of Carg05886 vs. ExPASy Swiss-Prot
Match: Q9FW03 (Putative methylesterase 11, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES11 PE=1 SV=1)

HSP 1 Score: 456.4 bits (1173), Expect = 3.5e-127
Identity = 250/441 (56.69%), Postives = 310/441 (70.29%), Query Frame = 0

Query: 1   MGNLCACLAPKS--MKKKP-TKRLHNPPPTTN----STKRW-TRVRSSRKEK----SDAV 60
           MGNLC+   P     K+KP TKR  +   +++    ++ RW  RVRSS   +     DA+
Sbjct: 1   MGNLCSLFTPPKPVKKRKPITKRQSSIGASSSGSGLNSNRWNNRVRSSSSRRDNKFEDAL 60

Query: 61  LTQEQVLAAAILFQQQNGRD---PFDRTTSVRYPKSGSKNSNTLPRSSSSRARALTDPLL 120
           + +  + AAA+LF+QQNG     PFDR+ S RY  S SK  N LPRSSSSR+R+ TDPLL
Sbjct: 61  IQEHALAAAAVLFRQQNGGGGSLPFDRSASQRYQGSCSK-KNQLPRSSSSRSRSSTDPLL 120

Query: 121 QPHQLVSENVKLEDIETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLAGSGVHSF 180
           QPHQ +++ +KL+D+ETNHFVLVHGG FGAWCWYKTIALLEE G+K TAIDLAG G++S 
Sbjct: 121 QPHQFLNQGIKLDDLETNHFVLVHGGSFGAWCWYKTIALLEEDGFKVTAIDLAGCGINSI 180

Query: 181 DPNRITSLLQYVHPLTDFLQMLPEGEKSPTFLLAHLLVSTFFGLILLAGTSLGICNILSP 240
           + N I SL QYV PLTD L+ LP GEK                                 
Sbjct: 181 NINGIASLSQYVKPLTDILEKLPIGEK--------------------------------- 240

Query: 241 PPVDIVLCGLSRLGFLFGLQVILVGHDFGGACIAYAMELFPFRISKAIFIAAAMLSNGQN 300
                               VILVGHDFGGACI+YAMELFP +ISKA+F+AAAML+NGQ+
Sbjct: 241 --------------------VILVGHDFGGACISYAMELFPSKISKAVFLAAAMLTNGQS 300

Query: 301 TLDMFSQQAGADDLMRQAQTFSYANGNDRPPTSINFEKSLLRDLFFNQCPAKDVALASVS 360
           TLDMFS +AG +DLMR+AQ F Y NGN+ PPT+I+ +KSLL+DL FNQ P+KDVALASVS
Sbjct: 301 TLDMFSLKAGQNDLMRKAQIFIYTNGNENPPTAIDLDKSLLKDLLFNQSPSKDVALASVS 360

Query: 361 MRPMPFAPILEKLCLTDLNYGLVKRFYIETPEDNAIPITLQDSMINKSPPQQVFRLKGAD 420
           MR +PFAP+LEKL L+D NYG V+R+YIET EDNAIP+TLQ++MIN SPP++V+RLKGAD
Sbjct: 361 MRSIPFAPVLEKLSLSDANYGSVRRYYIETLEDNAIPVTLQENMINSSPPEKVYRLKGAD 387

Query: 421 HSPFFSKPQALNKLLVEISKI 427
           H+PFFSKPQAL+KLL+EI++I
Sbjct: 421 HAPFFSKPQALHKLLLEIARI 387

BLAST of Carg05886 vs. ExPASy Swiss-Prot
Match: F4IE65 (Putative methylesterase 13, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES13 PE=2 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 3.6e-100
Identity = 197/406 (48.52%), Postives = 262/406 (64.53%), Query Frame = 0

Query: 44  KSDAVLTQEQVLAAAILFQQQNGRD-------PFDRTTSVRYP---------------KS 103
           + D+ + QEQ LAA  L   Q  R+        F R+TSV YP               ++
Sbjct: 86  EKDSHIIQEQTLAATNLLFNQTPRNSNSVVPPSFRRSTSVVYPSAQPSGTSSGPVSAVQT 145

Query: 104 GSKNSNTLPRSSSSRARALTDPLLQPHQLVSENV-KLEDIETNHFVLVHGGGFGAWCWYK 163
             K+S    RSSSSR R+ TDP+++P+QLV + + K+E  ET  FVLVHGGGFGAWCWYK
Sbjct: 146 PKKSSAGFVRSSSSRQRSSTDPMIKPNQLVDKELNKVEGSETKRFVLVHGGGFGAWCWYK 205

Query: 164 TIALLEEGGYKATAIDLAGSGVHSFDPNRITSLLQYVHPLTDFLQMLPEGEKSPTFLLAH 223
           TI LLE+ G++  A++L GSGV S D N ITSL  Y  PL  F + L   EK        
Sbjct: 206 TITLLEKHGFQVDAVELTGSGVSSIDTNNITSLAHYSKPLLHFFESLKPTEK-------- 265

Query: 224 LLVSTFFGLILLAGTSLGICNILSPPPVDIVLCGLSRLGFLFGLQVILVGHDFGGACIAY 283
                                                        VILVGHDFGGAC++Y
Sbjct: 266 ---------------------------------------------VILVGHDFGGACMSY 325

Query: 284 AMELFPFRISKAIFIAAAMLSNGQNTLDMFSQQAGADDLMRQAQTFSYANGNDRPPTSIN 343
           AME+FP +I+KA+FI+AAML+NGQ+TLD+F+QQ G++DLM+QAQ F YANG   PPT+++
Sbjct: 326 AMEMFPTKIAKAVFISAAMLANGQSTLDLFNQQLGSNDLMQQAQIFLYANGKKNPPTAVD 385

Query: 344 FEKSLLRDLFFNQCPAKDVALASVSMRPMPFAPILEKLCLTDLNYGLVKRFYIETPEDNA 403
           F++SLLRD  FNQ P KD+ALASVS+RP+PFAP+ EK+ +++ NYG ++RFYI+T ED A
Sbjct: 386 FDRSLLRDFLFNQSPPKDLALASVSIRPIPFAPVSEKVHVSEKNYGSIRRFYIKTMEDYA 438

Query: 404 IPITLQDSMINKSPPQQVFRLKGADHSPFFSKPQALNKLLVEISKI 427
           +P+ LQ++MI  +PP+QVF+LKG+DH+PFFS+PQ+LNK+LVEIS+I
Sbjct: 446 VPVLLQEAMIKLNPPEQVFQLKGSDHAPFFSRPQSLNKILVEISQI 438

BLAST of Carg05886 vs. ExPASy Swiss-Prot
Match: F4I0K9 (Putative methylesterase 15, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES15 PE=2 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 2.3e-99
Identity = 201/424 (47.41%), Postives = 271/424 (63.92%), Query Frame = 0

Query: 31  TKRWTRVRSSRKE-----KSDAVLTQEQVLAAAILFQQ----QNGRDPFDRTTSVRYPK- 90
           TK+  R R  +++     + D+++  + + A  ILF Q     N   PF R+TSV Y + 
Sbjct: 68  TKKKIRERHHQEQHHHDHEKDSLIQDQTLAATNILFSQTPRNSNSAPPFRRSTSVVYTQP 127

Query: 91  ------------SGS----KNSNTLPRSSSSRARALTDPLLQPHQLVSENVKLEDIETNH 150
                       SG+    K++    RSSS+R R+ TDP+L+P+QL+ + +K+E  ET  
Sbjct: 128 PTAAVAASVGSVSGALTPKKSTYGYVRSSSNRQRSSTDPVLKPNQLLDKELKVEGAETKR 187

Query: 151 FVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLAGSGVHSFDPNRITSLLQYVHPLTDFL 210
           FVLVHGGGFGAWCWYKTI LLE+ G++  A+DL GSGV SFD N ITSL QYV PL  F 
Sbjct: 188 FVLVHGGGFGAWCWYKTITLLEKHGFQVDAVDLTGSGVSSFDTNNITSLAQYVKPLLHFF 247

Query: 211 QMLPEGEKSPTFLLAHLLVSTFFGLILLAGTSLGICNILSPPPVDIVLCGLSRLGFLFGL 270
             L   EK                                                    
Sbjct: 248 DTLKPTEK---------------------------------------------------- 307

Query: 271 QVILVGHDFGGACIAYAMELFPFRISKAIFIAAAMLSNGQNTLDMFSQQAGAD-DLMRQA 330
            VILVGHDFGGAC++YAME++P +I+KAIFI+AAML+N Q+TLD+F+QQ  ++ DLM Q 
Sbjct: 308 -VILVGHDFGGACMSYAMEMYPSKIAKAIFISAAMLANAQSTLDLFNQQPDSNYDLMEQV 367

Query: 331 QTFSYANGNDRPPTSINFEKSLLRDLFFNQCPAKDVALASVSMRPMPFAPILEKLCLTDL 390
             F YANG   PPT+++F++SLLRD FFNQ P KDVALASVSMRP+PFAP++EKL +++ 
Sbjct: 368 HLFLYANGKKNPPTAVDFDRSLLRDFFFNQSPPKDVALASVSMRPIPFAPVVEKLHVSEK 427

Query: 391 NYGLVKRFYIETPEDN-AIPITLQDSMINKSPPQQVFRLKGADHSPFFSKPQALNKLLVE 427
           NYG ++RFYI+T ED+ A+P++LQD+MI  +PP+QVF LKG+DH+PFFS+PQ+LN++LVE
Sbjct: 428 NYGSIRRFYIKTMEDDYAVPVSLQDAMIKSNPPEQVFHLKGSDHAPFFSRPQSLNRILVE 438

BLAST of Carg05886 vs. ExPASy Swiss-Prot
Match: Q9FVW3 (Putative methylesterase 14, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES14 PE=1 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 1.1e-69
Identity = 152/390 (38.97%), Postives = 216/390 (55.38%), Query Frame = 0

Query: 38  RSSRKEKSDAVLTQEQVLAAAILFQQQNGRDPFDRTTSVRYPKSGSKNSNTLPRSSSSRA 97
           RS RK  +D  +   + L+ AI   Q + R  FD + S R              S+S+R 
Sbjct: 28  RSQRKLLADEEMLHRRALSMAIHQAQLSQR--FDGSMSRRV------------GSTSTRK 87

Query: 98  RALTDPLLQPHQLVSENVKLEDIETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDL 157
           R L+DP     Q+   +   E +    FVLVHG GFGAWCWYK +A LEE G     +DL
Sbjct: 88  RTLSDPFSNGKQVPDFS---ESLIVKKFVLVHGEGFGAWCWYKMVASLEESGLSPVTVDL 147

Query: 158 AGSGVHSFDPNRITSLLQYVHPLTDFLQMLPEGEKSPTFLLAHLLVSTFFGLILLAGTSL 217
            G G +  D N +++L +Y  PL D L+ LPE EK                         
Sbjct: 148 TGCGFNMTDTNTVSTLEEYSKPLIDLLENLPEEEK------------------------- 207

Query: 218 GICNILSPPPVDIVLCGLSRLGFLFGLQVILVGHDFGGACIAYAMELFPFRISKAIFIAA 277
                                       VILVGH  GGA I+YA+E FP +ISKAIF+ A
Sbjct: 208 ----------------------------VILVGHSTGGASISYALERFPEKISKAIFVCA 267

Query: 278 AMLSNGQNTLDMFSQQAG-ADDLMRQAQTFSYANGNDRPPTSINFEKSLLRDLFFNQCPA 337
            M+S+GQ   D+FS++ G A+  M+++Q   Y NG D+PPT   FEK  ++ L+FNQ P 
Sbjct: 268 TMVSDGQRPFDVFSEELGSAERFMKESQFLIYGNGKDKPPTGFMFEKPHMKGLYFNQSPN 327

Query: 338 KDVALASVSMRPMPFAPILEKLCLTDLNYGLVKRFYIETPEDNAIPITLQDSMINKSPPQ 397
           KD+ALA +SMRP+P  P++EK+ LT   YG  +RFY++T +D A+   +Q+ ++ ++ P+
Sbjct: 328 KDIALAMISMRPVPLGPMMEKVSLTAERYGKGRRFYVQTLDDRALSPDVQEKLVRENSPE 347

Query: 398 QVFRLKGADHSPFFSKPQALNKLLVEISKI 427
            VF++KG+DH PFFSKPQ+L+K+L+EI++I
Sbjct: 388 GVFKIKGSDHCPFFSKPQSLHKILLEIAQI 347

BLAST of Carg05886 vs. ExPASy Swiss-Prot
Match: Q940H7 (Putative methylesterase 12, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES12 PE=2 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 8.1e-68
Identity = 151/390 (38.72%), Postives = 214/390 (54.87%), Query Frame = 0

Query: 38  RSSRKEKSDAVLTQEQVLAAAILFQQQNGRDPFDRTTSVRYPKSGSKNSNTLPRSSSSRA 97
           RS RK  +D      + L+ AI   Q + R  FD + S R              S+SSR 
Sbjct: 29  RSQRKLLADEENLHRRALSMAIHQAQVSQR--FDGSMSRRI------------GSTSSRR 88

Query: 98  RALTDPLLQPHQLVSENVKLEDIETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDL 157
             L+D      Q+      LE ++   FVLVHG GFGAWCWYKTIA LEE G     +DL
Sbjct: 89  GTLSDSFSNNKQVPE---FLESLKVKKFVLVHGEGFGAWCWYKTIASLEESGLSPVTVDL 148

Query: 158 AGSGVHSFDPNRITSLLQYVHPLTDFLQMLPEGEKSPTFLLAHLLVSTFFGLILLAGTSL 217
           AGSG +  D N +++L +Y  PL + +Q LP  EK                         
Sbjct: 149 AGSGFNMTDANSVSTLEEYSKPLIELIQNLPAEEK------------------------- 208

Query: 218 GICNILSPPPVDIVLCGLSRLGFLFGLQVILVGHDFGGACIAYAMELFPFRISKAIFIAA 277
                                       VILVGH  GGAC++YA+E FP +ISKAIFI A
Sbjct: 209 ----------------------------VILVGHSTGGACVSYALERFPEKISKAIFICA 268

Query: 278 AMLSNGQNTLDMFSQQAG-ADDLMRQAQTFSYANGNDRPPTSINFEKSLLRDLFFNQCPA 337
            M+++GQ   D+F+ + G A+  M+++Q   Y NG D P T   FEK  ++ L+FNQ P 
Sbjct: 269 TMVTDGQRPFDVFADELGSAERFMKESQFLIYGNGKDNPATGFMFEKQHMKGLYFNQSPN 328

Query: 338 KDVALASVSMRPMPFAPILEKLCLTDLNYGLVKRFYIETPEDNAIPITLQDSMINKSPPQ 397
           KD+AL+ +SMRP+P  P++EKL L+   YG  +RFY++T +D A+   +Q+ ++ ++ P+
Sbjct: 329 KDIALSMISMRPVPLGPMMEKLSLSAERYGKGRRFYVQTLDDLALSPDVQEKLVRENSPE 348

Query: 398 QVFRLKGADHSPFFSKPQALNKLLVEISKI 427
            VF++KG+DH PFFSKPQ+L+K+L+EI++I
Sbjct: 389 AVFKIKGSDHCPFFSKPQSLHKILLEIAQI 348

BLAST of Carg05886 vs. ExPASy TrEMBL
Match: A0A6J1GR58 (putative methylesterase 11, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111456729 PE=4 SV=1)

HSP 1 Score: 724.5 bits (1869), Expect = 2.5e-205
Identity = 374/430 (86.98%), Postives = 375/430 (87.21%), Query Frame = 0

Query: 1   MGNLCACLAPKSMKKKPTKRLHNPPPTTNSTKRWTRVRSSRKEKSDAVLTQEQVLAAAIL 60
           MGNLCACLAPKSMKKKPTKRLHNPPPTTNSTKRWTRVR SRKEKSDA LTQEQVLAAAIL
Sbjct: 1   MGNLCACLAPKSMKKKPTKRLHNPPPTTNSTKRWTRVRFSRKEKSDAALTQEQVLAAAIL 60

Query: 61  FQQQNGRDPFDRTTSVRYPKSGSKNSNTLPRSSSSRARALTDPLLQPHQLVSENVKLEDI 120
           FQQQNGRDPFDRTTSVRYPKSGSKNSNTLPRSSSSRARALTDPLLQPHQLVSENVKLEDI
Sbjct: 61  FQQQNGRDPFDRTTSVRYPKSGSKNSNTLPRSSSSRARALTDPLLQPHQLVSENVKLEDI 120

Query: 121 ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLAGSGVHSFDPNRITSLLQYVHPL 180
           ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLAGSGVHSFDPNRITSLLQYVHPL
Sbjct: 121 ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLAGSGVHSFDPNRITSLLQYVHPL 180

Query: 181 TDFLQMLPEGEKSPTFLLAHLLVSTFFGLILLAGTSLGICNILSPPPVDIVLCGLSRLGF 240
           TDFLQMLPEGEK                                                
Sbjct: 181 TDFLQMLPEGEK------------------------------------------------ 240

Query: 241 LFGLQVILVGHDFGGACIAYAMELFPFRISKAIFIAAAMLSNGQNTLDMFSQQAGADDLM 300
                VILVGHDFGGACIAYAMELFPFRISKAIFIAAAMLSNGQNTLDMFSQQAGADDLM
Sbjct: 241 -----VILVGHDFGGACIAYAMELFPFRISKAIFIAAAMLSNGQNTLDMFSQQAGADDLM 300

Query: 301 RQAQTFSYANGNDRPPTSINFEKSLLRDLFFNQCPAKDVALASVSMRPMPFAPILEKLCL 360
           R+AQTFSYANGNDRPPTSINFEKSLLRDLFFNQCPAKDVALASVSMRPMPFAPILEKLCL
Sbjct: 301 RRAQTFSYANGNDRPPTSINFEKSLLRDLFFNQCPAKDVALASVSMRPMPFAPILEKLCL 360

Query: 361 TDLNYGLVKRFYIETPEDNAIPITLQDSMINKSPPQQVFRLKGADHSPFFSKPQALNKLL 420
           TDLNYGLVKRFYIETPEDNAIPITLQDSMINKSPPQQVFRLKGADHSPFFSKPQALNKLL
Sbjct: 361 TDLNYGLVKRFYIETPEDNAIPITLQDSMINKSPPQQVFRLKGADHSPFFSKPQALNKLL 377

Query: 421 VEISKIKTPS 431
           VEISKIKTPS
Sbjct: 421 VEISKIKTPS 377

BLAST of Carg05886 vs. ExPASy TrEMBL
Match: A0A6J1JUW7 (putative methylesterase 11, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111487718 PE=4 SV=1)

HSP 1 Score: 720.7 bits (1859), Expect = 3.6e-204
Identity = 372/430 (86.51%), Postives = 375/430 (87.21%), Query Frame = 0

Query: 1   MGNLCACLAPKSMKKKPTKRLHNPPPTTNSTKRWTRVRSSRKEKSDAVLTQEQVLAAAIL 60
           MGNLCACLAPKSMKKKPTKRL NPPPTTNS+KRWTRVRSSRKEKSDA LTQEQVLAAAIL
Sbjct: 1   MGNLCACLAPKSMKKKPTKRLRNPPPTTNSSKRWTRVRSSRKEKSDAALTQEQVLAAAIL 60

Query: 61  FQQQNGRDPFDRTTSVRYPKSGSKNSNTLPRSSSSRARALTDPLLQPHQLVSENVKLEDI 120
           FQQQNGRDPFDRTTSVRYPKSGSKNSNTLPRSSSSRARALTDPLLQPHQLVSENVKLEDI
Sbjct: 61  FQQQNGRDPFDRTTSVRYPKSGSKNSNTLPRSSSSRARALTDPLLQPHQLVSENVKLEDI 120

Query: 121 ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLAGSGVHSFDPNRITSLLQYVHPL 180
           ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLAGSGVHSFDPNRITSLLQYVHPL
Sbjct: 121 ETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLAGSGVHSFDPNRITSLLQYVHPL 180

Query: 181 TDFLQMLPEGEKSPTFLLAHLLVSTFFGLILLAGTSLGICNILSPPPVDIVLCGLSRLGF 240
           TDFLQMLPEGEK                                                
Sbjct: 181 TDFLQMLPEGEK------------------------------------------------ 240

Query: 241 LFGLQVILVGHDFGGACIAYAMELFPFRISKAIFIAAAMLSNGQNTLDMFSQQAGADDLM 300
                VILVGHDFGGACIA+AMELFP+RISKAIFIAAAMLSNGQNTLDMFSQQAGADDLM
Sbjct: 241 -----VILVGHDFGGACIAHAMELFPYRISKAIFIAAAMLSNGQNTLDMFSQQAGADDLM 300

Query: 301 RQAQTFSYANGNDRPPTSINFEKSLLRDLFFNQCPAKDVALASVSMRPMPFAPILEKLCL 360
           RQAQTFSYANGNDRPPTSINFEKSLLRDLFFNQCPAKDVALASVSMRPMPFAPILEKLCL
Sbjct: 301 RQAQTFSYANGNDRPPTSINFEKSLLRDLFFNQCPAKDVALASVSMRPMPFAPILEKLCL 360

Query: 361 TDLNYGLVKRFYIETPEDNAIPITLQDSMINKSPPQQVFRLKGADHSPFFSKPQALNKLL 420
           TDLNYGLVKRFYIETPEDNAIPITLQDSMINKSPPQQVFRLKGADHSPFFSKPQALNKLL
Sbjct: 361 TDLNYGLVKRFYIETPEDNAIPITLQDSMINKSPPQQVFRLKGADHSPFFSKPQALNKLL 377

Query: 421 VEISKIKTPS 431
           VEISKIKTPS
Sbjct: 421 VEISKIKTPS 377

BLAST of Carg05886 vs. ExPASy TrEMBL
Match: A0A1S3CBA3 (putative methylesterase 11, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103498536 PE=4 SV=1)

HSP 1 Score: 646.0 bits (1665), Expect = 1.1e-181
Identity = 338/433 (78.06%), Postives = 354/433 (81.76%), Query Frame = 0

Query: 1   MGNLCACLAPKSMKKKPTKRLHNPPPTTNSTKRWTRVRSSRKEKSDAVLTQEQVLAAAIL 60
           MGNLCACLAPKS+KKKPTKRL +P    NS+KRWTRVRSSRK+K DA LT+EQVLAAAIL
Sbjct: 1   MGNLCACLAPKSIKKKPTKRLPSPTLPNNSSKRWTRVRSSRKDKPDAALTREQVLAAAIL 60

Query: 61  F---QQQNGRDPFDRTTSVRYPKSGSKNSNTLPRSSSSRARALTDPLLQPHQLVSENVKL 120
           F   QQ N RDPFDRTTS+RYPKSGSKNSN LPRSSSSRAR+LTDPLLQPHQLVSENVK 
Sbjct: 61  FQQHQQHNARDPFDRTTSLRYPKSGSKNSNVLPRSSSSRARSLTDPLLQPHQLVSENVKP 120

Query: 121 EDIETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLAGSGVHSFDPNRITSLLQYV 180
           EDIETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDL GSGVHSFDPN ITSL QYV
Sbjct: 121 EDIETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLTGSGVHSFDPNCITSLSQYV 180

Query: 181 HPLTDFLQMLPEGEKSPTFLLAHLLVSTFFGLILLAGTSLGICNILSPPPVDIVLCGLSR 240
            PLTDFL+ L EGEK                                             
Sbjct: 181 QPLTDFLEKLAEGEK--------------------------------------------- 240

Query: 241 LGFLFGLQVILVGHDFGGACIAYAMELFPFRISKAIFIAAAMLSNGQNTLDMFSQQAGAD 300
                   VILVGHDFGGACIAYAMELFPFRI+KAIFIAAAML+NGQNTLDMFSQQAGAD
Sbjct: 241 --------VILVGHDFGGACIAYAMELFPFRIAKAIFIAAAMLTNGQNTLDMFSQQAGAD 300

Query: 301 DLMRQAQTFSYANGNDRPPTSINFEKSLLRDLFFNQCPAKDVALASVSMRPMPFAPILEK 360
           DLM+QAQTFSYANGN  PPT+IN EKSLLRDLFFNQ PAKDVALASVSMRP+PFAPILEK
Sbjct: 301 DLMQQAQTFSYANGNSHPPTAINLEKSLLRDLFFNQSPAKDVALASVSMRPIPFAPILEK 360

Query: 361 LCLTDLNYGLVKRFYIETPEDNAIPITLQDSMINKSPPQQVFRLKGADHSPFFSKPQALN 420
           LCL+DL YG V+RFYIETPEDNAIPITLQDSMINKSPPQQVFRLKGADHSPFFSKPQALN
Sbjct: 361 LCLSDLKYGSVRRFYIETPEDNAIPITLQDSMINKSPPQQVFRLKGADHSPFFSKPQALN 380

Query: 421 KLLVEISKIKTPS 431
           KLL+EISKIKTPS
Sbjct: 421 KLLIEISKIKTPS 380

BLAST of Carg05886 vs. ExPASy TrEMBL
Match: A0A5D3BLC2 (Putative methylesterase 11 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G002410 PE=4 SV=1)

HSP 1 Score: 645.6 bits (1664), Expect = 1.5e-181
Identity = 337/433 (77.83%), Postives = 355/433 (81.99%), Query Frame = 0

Query: 1   MGNLCACLAPKSMKKKPTKRLHNPPPTTNSTKRWTRVRSSRKEKSDAVLTQEQVLAAAIL 60
           MGNLCAC+APKS+KKKPTKRL +P    NS+KRWTRVRSSRK+K DA LT+EQVLAAAIL
Sbjct: 1   MGNLCACIAPKSIKKKPTKRLPSPTLPNNSSKRWTRVRSSRKDKPDAALTREQVLAAAIL 60

Query: 61  F---QQQNGRDPFDRTTSVRYPKSGSKNSNTLPRSSSSRARALTDPLLQPHQLVSENVKL 120
           F   QQ N RDPFDRTTS+RYPKSGSKNSN LPRSSSSRAR+LTDPLLQPHQLVSENVK 
Sbjct: 61  FQQHQQHNARDPFDRTTSLRYPKSGSKNSNVLPRSSSSRARSLTDPLLQPHQLVSENVKP 120

Query: 121 EDIETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLAGSGVHSFDPNRITSLLQYV 180
           EDIETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDL GSGVHSFDPN ITSL QYV
Sbjct: 121 EDIETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLTGSGVHSFDPNCITSLSQYV 180

Query: 181 HPLTDFLQMLPEGEKSPTFLLAHLLVSTFFGLILLAGTSLGICNILSPPPVDIVLCGLSR 240
            PLTDFL+ L EGEK                                             
Sbjct: 181 QPLTDFLEKLAEGEK--------------------------------------------- 240

Query: 241 LGFLFGLQVILVGHDFGGACIAYAMELFPFRISKAIFIAAAMLSNGQNTLDMFSQQAGAD 300
                   VILVGHDFGGACIAYAMELFPFRI+KAIFIAAAML+NGQNTLDMFSQQAGAD
Sbjct: 241 --------VILVGHDFGGACIAYAMELFPFRIAKAIFIAAAMLTNGQNTLDMFSQQAGAD 300

Query: 301 DLMRQAQTFSYANGNDRPPTSINFEKSLLRDLFFNQCPAKDVALASVSMRPMPFAPILEK 360
           DLM+QAQTFSYANGN+ PPT+IN EKSLLRDLFFNQ PAKDVALASVSMRP+PFAPILEK
Sbjct: 301 DLMQQAQTFSYANGNNHPPTAINLEKSLLRDLFFNQSPAKDVALASVSMRPIPFAPILEK 360

Query: 361 LCLTDLNYGLVKRFYIETPEDNAIPITLQDSMINKSPPQQVFRLKGADHSPFFSKPQALN 420
           LCL+DL YG V+RFYIETPEDNAIPITLQDSMINKSPPQQVFRLKGADHSPFFSKPQALN
Sbjct: 361 LCLSDLKYGSVRRFYIETPEDNAIPITLQDSMINKSPPQQVFRLKGADHSPFFSKPQALN 380

Query: 421 KLLVEISKIKTPS 431
           KLL+EISKIKTPS
Sbjct: 421 KLLIEISKIKTPS 380

BLAST of Carg05886 vs. ExPASy TrEMBL
Match: A0A0A0KSG7 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G640000 PE=4 SV=1)

HSP 1 Score: 645.2 bits (1663), Expect = 1.9e-181
Identity = 337/433 (77.83%), Postives = 353/433 (81.52%), Query Frame = 0

Query: 1   MGNLCACLAPKSMKKKPTKRLHNPPPTTNSTKRWTRVRSSRKEKSDAVLTQEQVLAAAIL 60
           MGN CACLAPKS+KKKP  RL NP   TNS+KRWTRVRSSRK+K DA LT++QVLAAAIL
Sbjct: 1   MGNFCACLAPKSIKKKPNHRLPNPTLPTNSSKRWTRVRSSRKDKPDAALTRDQVLAAAIL 60

Query: 61  FQQ---QNGRDPFDRTTSVRYPKSGSKNSNTLPRSSSSRARALTDPLLQPHQLVSENVKL 120
           FQQ    N RDPFDRTTS+RYPKSGSKNSN LPRSSSSRAR+LTDPLLQPHQLVSENVK 
Sbjct: 61  FQQHQHHNARDPFDRTTSLRYPKSGSKNSNALPRSSSSRARSLTDPLLQPHQLVSENVKP 120

Query: 121 EDIETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLAGSGVHSFDPNRITSLLQYV 180
           EDIETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDL GSGVHSFDPN ITSL QYV
Sbjct: 121 EDIETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLTGSGVHSFDPNCITSLSQYV 180

Query: 181 HPLTDFLQMLPEGEKSPTFLLAHLLVSTFFGLILLAGTSLGICNILSPPPVDIVLCGLSR 240
            PLTDFL+ LPEGEK                                             
Sbjct: 181 QPLTDFLEKLPEGEK--------------------------------------------- 240

Query: 241 LGFLFGLQVILVGHDFGGACIAYAMELFPFRISKAIFIAAAMLSNGQNTLDMFSQQAGAD 300
                   VILVGHDFGGACIAYAMELFPFRI+KAIFIAAAMLSNGQNT DMFSQQAGAD
Sbjct: 241 --------VILVGHDFGGACIAYAMELFPFRIAKAIFIAAAMLSNGQNTSDMFSQQAGAD 300

Query: 301 DLMRQAQTFSYANGNDRPPTSINFEKSLLRDLFFNQCPAKDVALASVSMRPMPFAPILEK 360
           DLM+QAQTFSYANGN+ PPT+IN EKSLLRDLFFNQ PAKDVALASVSMRP+PFAPILEK
Sbjct: 301 DLMQQAQTFSYANGNNHPPTAINLEKSLLRDLFFNQSPAKDVALASVSMRPIPFAPILEK 360

Query: 361 LCLTDLNYGLVKRFYIETPEDNAIPITLQDSMINKSPPQQVFRLKGADHSPFFSKPQALN 420
           LCL+DL YG V+RFYIETPEDNAIPITLQDSMINKSPPQQVFRLKGADHSPFFSKPQALN
Sbjct: 361 LCLSDLKYGSVRRFYIETPEDNAIPITLQDSMINKSPPQQVFRLKGADHSPFFSKPQALN 380

Query: 421 KLLVEISKIKTPS 431
           KLL+EISKIKTPS
Sbjct: 421 KLLIEISKIKTPS 380

BLAST of Carg05886 vs. TAIR 10
Match: AT3G29770.1 (methyl esterase 11 )

HSP 1 Score: 456.4 bits (1173), Expect = 2.5e-128
Identity = 250/441 (56.69%), Postives = 310/441 (70.29%), Query Frame = 0

Query: 1   MGNLCACLAPKS--MKKKP-TKRLHNPPPTTN----STKRW-TRVRSSRKEK----SDAV 60
           MGNLC+   P     K+KP TKR  +   +++    ++ RW  RVRSS   +     DA+
Sbjct: 1   MGNLCSLFTPPKPVKKRKPITKRQSSIGASSSGSGLNSNRWNNRVRSSSSRRDNKFEDAL 60

Query: 61  LTQEQVLAAAILFQQQNGRD---PFDRTTSVRYPKSGSKNSNTLPRSSSSRARALTDPLL 120
           + +  + AAA+LF+QQNG     PFDR+ S RY  S SK  N LPRSSSSR+R+ TDPLL
Sbjct: 61  IQEHALAAAAVLFRQQNGGGGSLPFDRSASQRYQGSCSK-KNQLPRSSSSRSRSSTDPLL 120

Query: 121 QPHQLVSENVKLEDIETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLAGSGVHSF 180
           QPHQ +++ +KL+D+ETNHFVLVHGG FGAWCWYKTIALLEE G+K TAIDLAG G++S 
Sbjct: 121 QPHQFLNQGIKLDDLETNHFVLVHGGSFGAWCWYKTIALLEEDGFKVTAIDLAGCGINSI 180

Query: 181 DPNRITSLLQYVHPLTDFLQMLPEGEKSPTFLLAHLLVSTFFGLILLAGTSLGICNILSP 240
           + N I SL QYV PLTD L+ LP GEK                                 
Sbjct: 181 NINGIASLSQYVKPLTDILEKLPIGEK--------------------------------- 240

Query: 241 PPVDIVLCGLSRLGFLFGLQVILVGHDFGGACIAYAMELFPFRISKAIFIAAAMLSNGQN 300
                               VILVGHDFGGACI+YAMELFP +ISKA+F+AAAML+NGQ+
Sbjct: 241 --------------------VILVGHDFGGACISYAMELFPSKISKAVFLAAAMLTNGQS 300

Query: 301 TLDMFSQQAGADDLMRQAQTFSYANGNDRPPTSINFEKSLLRDLFFNQCPAKDVALASVS 360
           TLDMFS +AG +DLMR+AQ F Y NGN+ PPT+I+ +KSLL+DL FNQ P+KDVALASVS
Sbjct: 301 TLDMFSLKAGQNDLMRKAQIFIYTNGNENPPTAIDLDKSLLKDLLFNQSPSKDVALASVS 360

Query: 361 MRPMPFAPILEKLCLTDLNYGLVKRFYIETPEDNAIPITLQDSMINKSPPQQVFRLKGAD 420
           MR +PFAP+LEKL L+D NYG V+R+YIET EDNAIP+TLQ++MIN SPP++V+RLKGAD
Sbjct: 361 MRSIPFAPVLEKLSLSDANYGSVRRYYIETLEDNAIPVTLQENMINSSPPEKVYRLKGAD 387

Query: 421 HSPFFSKPQALNKLLVEISKI 427
           H+PFFSKPQAL+KLL+EI++I
Sbjct: 421 HAPFFSKPQALHKLLLEIARI 387

BLAST of Carg05886 vs. TAIR 10
Match: AT1G26360.1 (methyl esterase 13 )

HSP 1 Score: 366.7 bits (940), Expect = 2.6e-101
Identity = 197/406 (48.52%), Postives = 262/406 (64.53%), Query Frame = 0

Query: 44  KSDAVLTQEQVLAAAILFQQQNGRD-------PFDRTTSVRYP---------------KS 103
           + D+ + QEQ LAA  L   Q  R+        F R+TSV YP               ++
Sbjct: 86  EKDSHIIQEQTLAATNLLFNQTPRNSNSVVPPSFRRSTSVVYPSAQPSGTSSGPVSAVQT 145

Query: 104 GSKNSNTLPRSSSSRARALTDPLLQPHQLVSENV-KLEDIETNHFVLVHGGGFGAWCWYK 163
             K+S    RSSSSR R+ TDP+++P+QLV + + K+E  ET  FVLVHGGGFGAWCWYK
Sbjct: 146 PKKSSAGFVRSSSSRQRSSTDPMIKPNQLVDKELNKVEGSETKRFVLVHGGGFGAWCWYK 205

Query: 164 TIALLEEGGYKATAIDLAGSGVHSFDPNRITSLLQYVHPLTDFLQMLPEGEKSPTFLLAH 223
           TI LLE+ G++  A++L GSGV S D N ITSL  Y  PL  F + L   EK        
Sbjct: 206 TITLLEKHGFQVDAVELTGSGVSSIDTNNITSLAHYSKPLLHFFESLKPTEK-------- 265

Query: 224 LLVSTFFGLILLAGTSLGICNILSPPPVDIVLCGLSRLGFLFGLQVILVGHDFGGACIAY 283
                                                        VILVGHDFGGAC++Y
Sbjct: 266 ---------------------------------------------VILVGHDFGGACMSY 325

Query: 284 AMELFPFRISKAIFIAAAMLSNGQNTLDMFSQQAGADDLMRQAQTFSYANGNDRPPTSIN 343
           AME+FP +I+KA+FI+AAML+NGQ+TLD+F+QQ G++DLM+QAQ F YANG   PPT+++
Sbjct: 326 AMEMFPTKIAKAVFISAAMLANGQSTLDLFNQQLGSNDLMQQAQIFLYANGKKNPPTAVD 385

Query: 344 FEKSLLRDLFFNQCPAKDVALASVSMRPMPFAPILEKLCLTDLNYGLVKRFYIETPEDNA 403
           F++SLLRD  FNQ P KD+ALASVS+RP+PFAP+ EK+ +++ NYG ++RFYI+T ED A
Sbjct: 386 FDRSLLRDFLFNQSPPKDLALASVSIRPIPFAPVSEKVHVSEKNYGSIRRFYIKTMEDYA 438

Query: 404 IPITLQDSMINKSPPQQVFRLKGADHSPFFSKPQALNKLLVEISKI 427
           +P+ LQ++MI  +PP+QVF+LKG+DH+PFFS+PQ+LNK+LVEIS+I
Sbjct: 446 VPVLLQEAMIKLNPPEQVFQLKGSDHAPFFSRPQSLNKILVEISQI 438

BLAST of Carg05886 vs. TAIR 10
Match: AT1G69240.1 (methyl esterase 15 )

HSP 1 Score: 364.0 bits (933), Expect = 1.7e-100
Identity = 201/424 (47.41%), Postives = 271/424 (63.92%), Query Frame = 0

Query: 31  TKRWTRVRSSRKE-----KSDAVLTQEQVLAAAILFQQ----QNGRDPFDRTTSVRYPK- 90
           TK+  R R  +++     + D+++  + + A  ILF Q     N   PF R+TSV Y + 
Sbjct: 68  TKKKIRERHHQEQHHHDHEKDSLIQDQTLAATNILFSQTPRNSNSAPPFRRSTSVVYTQP 127

Query: 91  ------------SGS----KNSNTLPRSSSSRARALTDPLLQPHQLVSENVKLEDIETNH 150
                       SG+    K++    RSSS+R R+ TDP+L+P+QL+ + +K+E  ET  
Sbjct: 128 PTAAVAASVGSVSGALTPKKSTYGYVRSSSNRQRSSTDPVLKPNQLLDKELKVEGAETKR 187

Query: 151 FVLVHGGGFGAWCWYKTIALLEEGGYKATAIDLAGSGVHSFDPNRITSLLQYVHPLTDFL 210
           FVLVHGGGFGAWCWYKTI LLE+ G++  A+DL GSGV SFD N ITSL QYV PL  F 
Sbjct: 188 FVLVHGGGFGAWCWYKTITLLEKHGFQVDAVDLTGSGVSSFDTNNITSLAQYVKPLLHFF 247

Query: 211 QMLPEGEKSPTFLLAHLLVSTFFGLILLAGTSLGICNILSPPPVDIVLCGLSRLGFLFGL 270
             L   EK                                                    
Sbjct: 248 DTLKPTEK---------------------------------------------------- 307

Query: 271 QVILVGHDFGGACIAYAMELFPFRISKAIFIAAAMLSNGQNTLDMFSQQAGAD-DLMRQA 330
            VILVGHDFGGAC++YAME++P +I+KAIFI+AAML+N Q+TLD+F+QQ  ++ DLM Q 
Sbjct: 308 -VILVGHDFGGACMSYAMEMYPSKIAKAIFISAAMLANAQSTLDLFNQQPDSNYDLMEQV 367

Query: 331 QTFSYANGNDRPPTSINFEKSLLRDLFFNQCPAKDVALASVSMRPMPFAPILEKLCLTDL 390
             F YANG   PPT+++F++SLLRD FFNQ P KDVALASVSMRP+PFAP++EKL +++ 
Sbjct: 368 HLFLYANGKKNPPTAVDFDRSLLRDFFFNQSPPKDVALASVSMRPIPFAPVVEKLHVSEK 427

Query: 391 NYGLVKRFYIETPEDN-AIPITLQDSMINKSPPQQVFRLKGADHSPFFSKPQALNKLLVE 427
           NYG ++RFYI+T ED+ A+P++LQD+MI  +PP+QVF LKG+DH+PFFS+PQ+LN++LVE
Sbjct: 428 NYGSIRRFYIKTMEDDYAVPVSLQDAMIKSNPPEQVFHLKGSDHAPFFSRPQSLNRILVE 438

BLAST of Carg05886 vs. TAIR 10
Match: AT1G33990.1 (methyl esterase 14 )

HSP 1 Score: 265.4 bits (677), Expect = 8.0e-71
Identity = 152/390 (38.97%), Postives = 216/390 (55.38%), Query Frame = 0

Query: 38  RSSRKEKSDAVLTQEQVLAAAILFQQQNGRDPFDRTTSVRYPKSGSKNSNTLPRSSSSRA 97
           RS RK  +D  +   + L+ AI   Q + R  FD + S R              S+S+R 
Sbjct: 28  RSQRKLLADEEMLHRRALSMAIHQAQLSQR--FDGSMSRRV------------GSTSTRK 87

Query: 98  RALTDPLLQPHQLVSENVKLEDIETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDL 157
           R L+DP     Q+   +   E +    FVLVHG GFGAWCWYK +A LEE G     +DL
Sbjct: 88  RTLSDPFSNGKQVPDFS---ESLIVKKFVLVHGEGFGAWCWYKMVASLEESGLSPVTVDL 147

Query: 158 AGSGVHSFDPNRITSLLQYVHPLTDFLQMLPEGEKSPTFLLAHLLVSTFFGLILLAGTSL 217
            G G +  D N +++L +Y  PL D L+ LPE EK                         
Sbjct: 148 TGCGFNMTDTNTVSTLEEYSKPLIDLLENLPEEEK------------------------- 207

Query: 218 GICNILSPPPVDIVLCGLSRLGFLFGLQVILVGHDFGGACIAYAMELFPFRISKAIFIAA 277
                                       VILVGH  GGA I+YA+E FP +ISKAIF+ A
Sbjct: 208 ----------------------------VILVGHSTGGASISYALERFPEKISKAIFVCA 267

Query: 278 AMLSNGQNTLDMFSQQAG-ADDLMRQAQTFSYANGNDRPPTSINFEKSLLRDLFFNQCPA 337
            M+S+GQ   D+FS++ G A+  M+++Q   Y NG D+PPT   FEK  ++ L+FNQ P 
Sbjct: 268 TMVSDGQRPFDVFSEELGSAERFMKESQFLIYGNGKDKPPTGFMFEKPHMKGLYFNQSPN 327

Query: 338 KDVALASVSMRPMPFAPILEKLCLTDLNYGLVKRFYIETPEDNAIPITLQDSMINKSPPQ 397
           KD+ALA +SMRP+P  P++EK+ LT   YG  +RFY++T +D A+   +Q+ ++ ++ P+
Sbjct: 328 KDIALAMISMRPVPLGPMMEKVSLTAERYGKGRRFYVQTLDDRALSPDVQEKLVRENSPE 347

Query: 398 QVFRLKGADHSPFFSKPQALNKLLVEISKI 427
            VF++KG+DH PFFSKPQ+L+K+L+EI++I
Sbjct: 388 GVFKIKGSDHCPFFSKPQSLHKILLEIAQI 347

BLAST of Carg05886 vs. TAIR 10
Match: AT4G09900.1 (methyl esterase 12 )

HSP 1 Score: 259.2 bits (661), Expect = 5.8e-69
Identity = 151/390 (38.72%), Postives = 214/390 (54.87%), Query Frame = 0

Query: 38  RSSRKEKSDAVLTQEQVLAAAILFQQQNGRDPFDRTTSVRYPKSGSKNSNTLPRSSSSRA 97
           RS RK  +D      + L+ AI   Q + R  FD + S R              S+SSR 
Sbjct: 29  RSQRKLLADEENLHRRALSMAIHQAQVSQR--FDGSMSRRI------------GSTSSRR 88

Query: 98  RALTDPLLQPHQLVSENVKLEDIETNHFVLVHGGGFGAWCWYKTIALLEEGGYKATAIDL 157
             L+D      Q+      LE ++   FVLVHG GFGAWCWYKTIA LEE G     +DL
Sbjct: 89  GTLSDSFSNNKQVPE---FLESLKVKKFVLVHGEGFGAWCWYKTIASLEESGLSPVTVDL 148

Query: 158 AGSGVHSFDPNRITSLLQYVHPLTDFLQMLPEGEKSPTFLLAHLLVSTFFGLILLAGTSL 217
           AGSG +  D N +++L +Y  PL + +Q LP  EK                         
Sbjct: 149 AGSGFNMTDANSVSTLEEYSKPLIELIQNLPAEEK------------------------- 208

Query: 218 GICNILSPPPVDIVLCGLSRLGFLFGLQVILVGHDFGGACIAYAMELFPFRISKAIFIAA 277
                                       VILVGH  GGAC++YA+E FP +ISKAIFI A
Sbjct: 209 ----------------------------VILVGHSTGGACVSYALERFPEKISKAIFICA 268

Query: 278 AMLSNGQNTLDMFSQQAG-ADDLMRQAQTFSYANGNDRPPTSINFEKSLLRDLFFNQCPA 337
            M+++GQ   D+F+ + G A+  M+++Q   Y NG D P T   FEK  ++ L+FNQ P 
Sbjct: 269 TMVTDGQRPFDVFADELGSAERFMKESQFLIYGNGKDNPATGFMFEKQHMKGLYFNQSPN 328

Query: 338 KDVALASVSMRPMPFAPILEKLCLTDLNYGLVKRFYIETPEDNAIPITLQDSMINKSPPQ 397
           KD+AL+ +SMRP+P  P++EKL L+   YG  +RFY++T +D A+   +Q+ ++ ++ P+
Sbjct: 329 KDIALSMISMRPVPLGPMMEKLSLSAERYGKGRRFYVQTLDDLALSPDVQEKLVRENSPE 348

Query: 398 QVFRLKGADHSPFFSKPQALNKLLVEISKI 427
            VF++KG+DH PFFSKPQ+L+K+L+EI++I
Sbjct: 389 AVFKIKGSDHCPFFSKPQSLHKILLEIAQI 348

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7033209.11.5e-244100.00putative methylesterase 11, chloroplastic, partial [Cucurbita argyrosperma subsp... [more]
XP_023538126.11.2e-20687.67putative methylesterase 11, chloroplastic [Cucurbita pepo subsp. pepo][more]
XP_022954463.15.2e-20586.98putative methylesterase 11, chloroplastic [Cucurbita moschata][more]
XP_022990993.17.5e-20486.51putative methylesterase 11, chloroplastic [Cucurbita maxima][more]
XP_038891023.11.2e-18278.06putative methylesterase 11, chloroplastic [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9FW033.5e-12756.69Putative methylesterase 11, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES... [more]
F4IE653.6e-10048.52Putative methylesterase 13, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES... [more]
F4I0K92.3e-9947.41Putative methylesterase 15, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES... [more]
Q9FVW31.1e-6938.97Putative methylesterase 14, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES... [more]
Q940H78.1e-6838.72Putative methylesterase 12, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES... [more]
Match NameE-valueIdentityDescription
A0A6J1GR582.5e-20586.98putative methylesterase 11, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1JUW73.6e-20486.51putative methylesterase 11, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A1S3CBA31.1e-18178.06putative methylesterase 11, chloroplastic OS=Cucumis melo OX=3656 GN=LOC10349853... [more]
A0A5D3BLC21.5e-18177.83Putative methylesterase 11 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A0A0KSG71.9e-18177.83AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G640... [more]
Match NameE-valueIdentityDescription
AT3G29770.12.5e-12856.69methyl esterase 11 [more]
AT1G26360.12.6e-10148.52methyl esterase 13 [more]
AT1G69240.11.7e-10047.41methyl esterase 15 [more]
AT1G33990.18.0e-7138.97methyl esterase 14 [more]
AT4G09900.15.8e-6938.72methyl esterase 12 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PFAMPF12697Abhydrolase_6coord: 125..417
e-value: 1.4E-13
score: 51.9
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 117..425
e-value: 2.4E-60
score: 206.5
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 124..422
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 67..96
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..47
NoneNo IPR availablePANTHERPTHR10992METHYLESTERASE FAMILY MEMBERcoord: 44..192
NoneNo IPR availablePANTHERPTHR10992METHYLESTERASE FAMILY MEMBERcoord: 245..426
NoneNo IPR availablePANTHERPTHR10992:SF872METHYLESTERASE 11, CHLOROPLASTIC-RELATEDcoord: 245..426
coord: 44..192

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg05886-RACarg05886-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009694 jasmonic acid metabolic process
biological_process GO:0009696 salicylic acid metabolic process
molecular_function GO:0080030 methyl indole-3-acetate esterase activity
molecular_function GO:0080032 methyl jasmonate esterase activity
molecular_function GO:0080031 methyl salicylate esterase activity