Carg05852 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg05852
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	basic helix-loop-helix (bHLH) DNA-binding superfamily protein
Location	Carg_Chr16: 4862855 .. 4864567 (+)
RNA-Seq Expression	Carg05852
Synteny	Carg05852

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCGGCTCGGCAGCGAGTGCGGAGATTTGGCCGTTGGTTAATGTTGGTAGAAATCCACCGCGTGCTAAAGATCACGTTCTGGCTGAGAGGAAACGCCGGGAGAAACTCAGCCAGAGATTCATTGCTCTTTCAGCCCTCTTACCTGGCCTTAAGAAGGTACCCAAATTATACTAATTTTACCTTTAATTAGGCGTAATATCAAATCATCCACTTTTTAACTATAAGTTTTCGTGACTTTACTTTGAGTTTTCTCAAAAAATTCTCGTACCAATAGAGATTCTTTATACGCTCAAAGTGAATAATCATATCATTGTAGAGAGTCAGTGATTCTTTACTTATAAACCCATGACCATACCTTTACGTTAGTCATGTCGATAGAATTCTCAAATTTTGAACAAAGAATTGTGAGCCTCGAACGTGTAGTCAAAAGTGACTAAAATATCAAACAAAAGGTGTACTTTGTTTGAGGGCTCAAGAGAAGGAATCGAGCCTTGATTAAGGGGAGACTATTCTAGGGCTCCATAGACCTCGAAGGAGGCTCTATGATGTACTTTGTTCGAGAAGAGAATTGGTGAGACAGAGTCCCACATAAATTAATTAAGAGGATGATCATGAGTTTATAAGCAACGAGGTCTACCTAATGCTAGATGTAGAGGTGCATAATAGATCGATATAAACATTATGAGCTGTCCCGTAATAAAATCTGTTGTTGTTGATACTTTTTTCTCGGTTCCTTCTTCCATAACCCGAGATTGGAGAAGGAAGAGATAACTTTTTCTTTTCCATTGCAAGGATTATTATATATATGATAATTTTTTCACTTTCCATATATATATATATATATAGAAATAAAAACAGATAGACTAGAAACAAACATCACTTATCTCAATGACACACCCGTATATTAAATGAATGGAATTGGGATAGGGATGGAATACAATGAAATAGAGCCATTTTGAGGTTTTCTATGAAATGGGACATGAAACGGGGCATGAAACGGAGCCACTACGAAGAAATACATCTCCAATGGGGCGAAGTCACGAAAGTTTATTGCTCACAATGGACAATATCATATCGTTAGGAGAGTCATTATTGACCCATATCCATGATAGATTCATTATTATAATTTGTTTTACAGATGGATAAAGCCTCAATTCTAGGAGATGCAATCAAGTACATAAAACAGCTACAAGAACGTGTGGAATCACTAGAGGAAATGGGCAAGAACAGCAACATTGAATCAGCCATTTTCATAAAGAAATCCCATTTATGTAACAACATGAATGATTGTTGTGATGATATAGATGGCGAAGAACACAATCACAAAACTGGATTTTCCGACCAAAAACTAGTGGAAATCGAAGCCAAAATCTCAGACAAACATGTTCTTGTTCGAATCCACTGTGAGAAACATGAGGGTTTGTTGGCGAAGTTATTAGAGGAAATGGAGAAGCTCAATTTTAAGATCATTAATGTCAATGTCTTCCCCTTTGGCAGCTCAACCCAATATATGGTTTTTCTTCTTCAGGTTCCTTCTAACACCATCTCTTATTCAATTAATTCCATCAATTTATGCATCCAATTACCATTTTTTCTATCTAATTATGAATATATTCTCTTTCGTCGCAGAGAAATTCGGACTTAAATATAGAATCAAAAGAGCTCGTACGAAAGTTAGAAGATGAATTGTTCAAGTTCATAACAACATAA

mRNA sequence

ATGTCCGGCTCGGCAGCGAGTGCGGAGATTTGGCCGTTGGTTAATGTTGGTAGAAATCCACCGCGTGCTAAAGATCACGTTCTGGCTGAGAGGAAACGCCGGGAGAAACTCAGCCAGAGATTCATTGCTCTTTCAGCCCTCTTACCTGGCCTTAAGAAGATGGATAAAGCCTCAATTCTAGGAGATGCAATCAAGTACATAAAACAGCTACAAGAACGTGTGGAATCACTAGAGGAAATGGGCAAGAACAGCAACATTGAATCAGCCATTTTCATAAAGAAATCCCATTTATGTAACAACATGAATGATTGTTGTGATGATATAGATGGCGAAGAACACAATCACAAAACTGGATTTTCCGACCAAAAACTAGTGGAAATCGAAGCCAAAATCTCAGACAAACATGTTCTTGTTCGAATCCACTGTGAGAAACATGAGGGTTTGTTGGCGAAGTTATTAGAGGAAATGGAGAAGCTCAATTTTAAGATCATTAATGTCAATGTCTTCCCCTTTGGCAGCTCAACCCAATATATGGTTTTTCTTCTTCAGAGAAATTCGGACTTAAATATAGAATCAAAAGAGCTCGTACGAAAGTTAGAAGATGAATTGTTCAAGTTCATAACAACATAA

Coding sequence (CDS)

ATGTCCGGCTCGGCAGCGAGTGCGGAGATTTGGCCGTTGGTTAATGTTGGTAGAAATCCACCGCGTGCTAAAGATCACGTTCTGGCTGAGAGGAAACGCCGGGAGAAACTCAGCCAGAGATTCATTGCTCTTTCAGCCCTCTTACCTGGCCTTAAGAAGATGGATAAAGCCTCAATTCTAGGAGATGCAATCAAGTACATAAAACAGCTACAAGAACGTGTGGAATCACTAGAGGAAATGGGCAAGAACAGCAACATTGAATCAGCCATTTTCATAAAGAAATCCCATTTATGTAACAACATGAATGATTGTTGTGATGATATAGATGGCGAAGAACACAATCACAAAACTGGATTTTCCGACCAAAAACTAGTGGAAATCGAAGCCAAAATCTCAGACAAACATGTTCTTGTTCGAATCCACTGTGAGAAACATGAGGGTTTGTTGGCGAAGTTATTAGAGGAAATGGAGAAGCTCAATTTTAAGATCATTAATGTCAATGTCTTCCCCTTTGGCAGCTCAACCCAATATATGGTTTTTCTTCTTCAGAGAAATTCGGACTTAAATATAGAATCAAAAGAGCTCGTACGAAAGTTAGAAGATGAATTGTTCAAGTTCATAACAACATAA

Protein sequence

MSGSAASAEIWPLVNVGRNPPRAKDHVLAERKRREKLSQRFIALSALLPGLKKMDKASILGDAIKYIKQLQERVESLEEMGKNSNIESAIFIKKSHLCNNMNDCCDDIDGEEHNHKTGFSDQKLVEIEAKISDKHVLVRIHCEKHEGLLAKLLEEMEKLNFKIINVNVFPFGSSTQYMVFLLQRNSDLNIESKELVRKLEDELFKFITT

Homology

[Close]

BLAST of Carg05852 vs. NCBI nr
Match: KAG7015418.1 (Transcription factor bHLH25, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 408.3 bits (1048), Expect = 4.0e-110
Identity = 209/209 (100.00%), Postives = 209/209 (100.00%), Query Frame = 0


Query: 1   MSGSAASAEIWPLVNVGRNPPRAKDHVLAERKRREKLSQRFIALSALLPGLKKMDKASIL 60
           MSGSAASAEIWPLVNVGRNPPRAKDHVLAERKRREKLSQRFIALSALLPGLKKMDKASIL
Sbjct: 1   MSGSAASAEIWPLVNVGRNPPRAKDHVLAERKRREKLSQRFIALSALLPGLKKMDKASIL 60

Query: 61  GDAIKYIKQLQERVESLEEMGKNSNIESAIFIKKSHLCNNMNDCCDDIDGEEHNHKTGFS 120
           GDAIKYIKQLQERVESLEEMGKNSNIESAIFIKKSHLCNNMNDCCDDIDGEEHNHKTGFS
Sbjct: 61  GDAIKYIKQLQERVESLEEMGKNSNIESAIFIKKSHLCNNMNDCCDDIDGEEHNHKTGFS 120

Query: 121 DQKLVEIEAKISDKHVLVRIHCEKHEGLLAKLLEEMEKLNFKIINVNVFPFGSSTQYMVF 180
           DQKLVEIEAKISDKHVLVRIHCEKHEGLLAKLLEEMEKLNFKIINVNVFPFGSSTQYMVF
Sbjct: 121 DQKLVEIEAKISDKHVLVRIHCEKHEGLLAKLLEEMEKLNFKIINVNVFPFGSSTQYMVF 180

Query: 181 LLQRNSDLNIESKELVRKLEDELFKFITT 210
           LLQRNSDLNIESKELVRKLEDELFKFITT
Sbjct: 181 LLQRNSDLNIESKELVRKLEDELFKFITT 209

[Close]

BLAST of Carg05852 vs. NCBI nr
Match: XP_023552894.1 (transcription factor bHLH25-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 394.8 bits (1013), Expect = 4.6e-106
Identity = 201/205 (98.05%), Postives = 204/205 (99.51%), Query Frame = 0


Query: 4   SAASAEIWPLVNVGRNPPRAKDHVLAERKRREKLSQRFIALSALLPGLKKMDKASILGDA 63
           +AA+AEIWPLVNVGRNPPRAKDHVLAERKRREKLSQRFIALSALLPGLKKMDKASILGDA
Sbjct: 61  AAAAAEIWPLVNVGRNPPRAKDHVLAERKRREKLSQRFIALSALLPGLKKMDKASILGDA 120

Query: 64  IKYIKQLQERVESLEEMGKNSNIESAIFIKKSHLCNNMNDCCDDIDGEEHNHKTGFSDQK 123
           IKYIKQLQERVESLEEMGKNSNIE+AI IKKSHLCNNMNDCCDDIDGEEHNHKTGFSDQK
Sbjct: 121 IKYIKQLQERVESLEEMGKNSNIEAAILIKKSHLCNNMNDCCDDIDGEEHNHKTGFSDQK 180

Query: 124 LVEIEAKISDKHVLVRIHCEKHEGLLAKLLEEMEKLNFKIINVNVFPFGSSTQYMVFLLQ 183
           LVEIEAKISDKHVLVRIHCEKHEGLLAKLLEEMEKLNFKIINVNVFPFGSSTQYMVFLLQ
Sbjct: 181 LVEIEAKISDKHVLVRIHCEKHEGLLAKLLEEMEKLNFKIINVNVFPFGSSTQYMVFLLQ 240

Query: 184 RNSDLNIESKELVRKLEDELFKFIT 209
           RNSDLNIESKELVRKLEDELFKFIT
Sbjct: 241 RNSDLNIESKELVRKLEDELFKFIT 265

[Close]

BLAST of Carg05852 vs. NCBI nr
Match: XP_022929215.1 (transcription factor bHLH25-like [Cucurbita moschata])

HSP 1 Score: 390.6 bits (1002), Expect = 8.6e-105
Identity = 199/204 (97.55%), Postives = 201/204 (98.53%), Query Frame = 0


Query: 6   ASAEIWPLVNVGRNPPRAKDHVLAERKRREKLSQRFIALSALLPGLKKMDKASILGDAIK 65
           A+AEIWPLVNVGRNPPRAKDHVLAERKRREKLSQRFIALSALLPGLKKMDKASILGDAIK
Sbjct: 61  AAAEIWPLVNVGRNPPRAKDHVLAERKRREKLSQRFIALSALLPGLKKMDKASILGDAIK 120

Query: 66  YIKQLQERVESLEEMGKNSNIESAIFIKKSHLCNNMNDCCDDIDGEEHNHKTGFSDQKLV 125
           YIKQLQERVESLEEMGKNSNIESAIFIKKSHLCNNMN CCDDIDGEEHNHKTGFSDQKLV
Sbjct: 121 YIKQLQERVESLEEMGKNSNIESAIFIKKSHLCNNMNGCCDDIDGEEHNHKTGFSDQKLV 180

Query: 126 EIEAKISDKHVLVRIHCEKHEGLLAKLLEEMEKLNFKIINVNVFPFGSSTQYMVFLLQRN 185
           E+EAKISDKHVLVRIHCEKHEGLLAKLLEEMEKLNFKIIN NVFPFGSSTQYMVFLLQRN
Sbjct: 181 EMEAKISDKHVLVRIHCEKHEGLLAKLLEEMEKLNFKIINGNVFPFGSSTQYMVFLLQRN 240

Query: 186 SDLNIESKELVRKLEDELFKFITT 210
           SD NIESKELVRKLEDELFKFITT
Sbjct: 241 SDFNIESKELVRKLEDELFKFITT 264

[Close]

BLAST of Carg05852 vs. NCBI nr
Match: XP_022985138.1 (transcription factor bHLH25-like [Cucurbita maxima])

HSP 1 Score: 367.9 bits (943), Expect = 6.0e-98
Identity = 191/205 (93.17%), Postives = 197/205 (96.10%), Query Frame = 0


Query: 5   AASAEIWPLVNVGRNPPRAKDHVLAERKRREKLSQRFIALSALLPGLKKMDKASILGDAI 64
           AA+AEIWPLVNVGRNPPRAKDHVLAERKRREKLSQRFIALSALLPGLKKMDKASILGDAI
Sbjct: 62  AAAAEIWPLVNVGRNPPRAKDHVLAERKRREKLSQRFIALSALLPGLKKMDKASILGDAI 121

Query: 65  KYIKQLQERVESLEEMGKNSNIESAIFIKKSHLCNNMNDCCDDIDGEEHNHKTGFSDQKL 124
           KYIKQLQERVESLEEMGKNSNIESAI IKKSHLCNNMND CDDIDGEE NHKT FSDQKL
Sbjct: 122 KYIKQLQERVESLEEMGKNSNIESAILIKKSHLCNNMND-CDDIDGEEQNHKTRFSDQKL 181

Query: 125 VEIEAKISDKHVLVRIHCEKHEGLLAKLLEEMEKLNFKIINVNVFPFGSSTQYMVFLLQR 184
           VEIEAKISDKHVLVRIHCEKHEGLLA+LLEEMEKLNF+IIN NVFPFGSSTQYMVFLLQR
Sbjct: 182 VEIEAKISDKHVLVRIHCEKHEGLLARLLEEMEKLNFRIINANVFPFGSSTQYMVFLLQR 241

Query: 185 NSDLNIESKELVRKLEDELFKFITT 210
           NS+  I+SKEL+RKLEDEL KFITT
Sbjct: 242 NSNSFIDSKELIRKLEDELVKFITT 265

[Close]

BLAST of Carg05852 vs. NCBI nr
Match: XP_022136751.1 (transcription factor bHLH18-like isoform X1 [Momordica charantia])

HSP 1 Score: 212.2 bits (539), Expect = 4.2e-51
Identity = 123/185 (66.49%), Postives = 142/185 (76.76%), Query Frame = 0


Query: 23  AKDHVLAERKRREKLSQRFIALSALLPGLKKMDKASILGDAIKYIKQLQERVESLEEMGK 82
           A+DHVLAERKRREKLSQRFIALSALLPGLKKMDKAS+LGDAIKYIKQ++ERV  LEE  K
Sbjct: 61  AQDHVLAERKRREKLSQRFIALSALLPGLKKMDKASVLGDAIKYIKQVEERVRVLEEKAK 120

Query: 83  NSNIESAIFIKKSHLCNNMNDCCDDIDGEEHNHKTGFSDQK-LVEIEAKISD--KHVLVR 142
             +IESAI + KSHL    NDC ++I        + FSDQK L EIEA+ISD   +VLVR
Sbjct: 121 KRSIESAILVNKSHL---YNDCDEEI--------SRFSDQKQLPEIEARISDDKNNVLVR 180

Query: 143 IHCEKHEGLLAKLLEEMEKLNFKIINVNVFPFGSSTQYMVFLLQRNSDLNIESKELVRKL 202
           IHCEK+EGL AKLL EMEK+N  I+N N FPFG+STQYMV + Q +S+L I+SKE   KL
Sbjct: 181 IHCEKNEGLFAKLLGEMEKMNLTIVNANFFPFGTSTQYMVLVAQIDSELKIKSKEGTNKL 234

Query: 203 EDELF 205
            D  F
Sbjct: 241 VDIKF 234

[Close]

BLAST of Carg05852 vs. ExPASy Swiss-Prot
Match: Q9T072 (Transcription factor bHLH25 OS=Arabidopsis thaliana OX=3702 GN=BHLH25 PE=2 SV=2)

HSP 1 Score: 161.0 bits (406), Expect = 1.5e-38
Identity = 93/190 (48.95%), Postives = 127/190 (66.84%), Query Frame = 0


Query: 18  RNPPRAKDHVLAERKRREKLSQRFIALSALLPGLKKMDKASILGDAIKYIKQLQERVESL 77
           RN   A+DH++AERKRREKL+QRF+ALSAL+PGLKKMDKAS+LGDA+K+IK LQERV  L
Sbjct: 145 RNQSNAQDHIIAERKRREKLTQRFVALSALVPGLKKMDKASVLGDALKHIKYLQERVGEL 204

Query: 78  EEMGKNSNIESAIFIKKSHLCNNMNDCCDDIDGEEHNHKTGFSDQKLVEIEAKISDKHVL 137
           EE  K   +ES + +KKS L  + N+       E+     GFSD  L EIE + SD+ VL
Sbjct: 205 EEQKKERRLESMVLVKKSKLILDDNNQSFSSSCED-----GFSDLDLPEIEVRFSDEDVL 264

Query: 138 VRIHCEKHEGLLAKLLEEMEKLNFKIINVNVFPFGSSTQYMVFLLQRNSDLNIESKELVR 197
           ++I CEK +G LAK++ E+EKL+  I N +V  FG  T  +  + ++ SD ++   ++V+
Sbjct: 265 IKILCEKQKGHLAKIMAEIEKLHILITNSSVLNFG-PTLDITIIAKKESDFDMTLMDVVK 324

Query: 198 KLEDELFKFI 208
            L   L  FI
Sbjct: 325 SLRSALSNFI 328

[Close]

BLAST of Carg05852 vs. ExPASy Swiss-Prot
Match: Q1PF17 (Transcription factor bHLH18 OS=Arabidopsis thaliana OX=3702 GN=BHLH18 PE=1 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 1.0e-36
Identity = 87/188 (46.28%), Postives = 124/188 (65.96%), Query Frame = 0


Query: 18  RNPPRAKDHVLAERKRREKLSQRFIALSALLPGLKKMDKASILGDAIKYIKQLQERVESL 77
           R+   A+DH+LAERKRREKL+QRF+ALSAL+PGLKKMDKAS+LGDAIK+IK LQE V+  
Sbjct: 119 RSQSNAQDHILAERKRREKLTQRFVALSALIPGLKKMDKASVLGDAIKHIKYLQESVKEY 178

Query: 78  EEMGKNSNIESAIFIKKSHLCNNMNDCCDDIDGEEHNHKTGFSDQKLVEIEAKISDKHVL 137
           EE  K   +ES + +KKS L  + N         + N  +  S   L EIE ++S K VL
Sbjct: 179 EEQKKEKTMESVVLVKKSSLVLDENHQPSSSSSSDGNRNS--SSSNLPEIEVRVSGKDVL 238

Query: 138 VRIHCEKHEGLLAKLLEEMEKLNFKIINVNVFPFGSSTQYMVFLLQRNSDLNIESKELVR 197
           ++I CEK +G + K++ E+EKL   I N NV PFG +    + + Q+N++ +++ +++V+
Sbjct: 239 IKILCEKQKGNVIKIMGEIEKLGLSITNSNVLPFGPTFDISI-IAQKNNNFDMKIEDVVK 298

Query: 198 KLEDELFK 206
            L   L K
Sbjct: 299 NLSFGLSK 303

[Close]

BLAST of Carg05852 vs. ExPASy Swiss-Prot
Match: Q8S3F1 (Transcription factor NAI1 OS=Arabidopsis thaliana OX=3702 GN=NAI1 PE=2 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 1.1e-30
Identity = 76/190 (40.00%), Postives = 119/190 (62.63%), Query Frame = 0


Query: 18  RNPPRAKDHVLAERKRREKLSQRFIALSALLPGLKKMDKASILGDAIKYIKQLQERVESL 77
           R P   K+HVLAERKRR+KL++R IALSALLPGLKK DKA++L DAIK++KQLQERV+ L
Sbjct: 125 REPHLLKEHVLAERKRRQKLNERLIALSALLPGLKKTDKATVLEDAIKHLKQLQERVKKL 184

Query: 78  EE--MGKNSNIESAIFIKKSHL------CNNMNDCCDDIDGEEHNHKTGFSDQKLVEIEA 137
           EE  +      +S I +K+S +       +  + C         + +     Q +  IEA
Sbjct: 185 EEERVVTKKMDQSIILVKRSQVYLDDDSSSYSSTCSAASPLSSSSDEVSIFKQTMPMIEA 244

Query: 138 KISDKHVLVRIHCEKHEGLLAKLLEEMEKLNFKIINVNVFPFGSSTQYMVFLLQRNSDLN 197
           ++SD+ +L+R+HCEK++G + K+L  +EK   +++N    PFG+ST  +  L + ++  +
Sbjct: 245 RVSDRDLLIRVHCEKNKGCMIKILSSLEKFRLEVVNSFTLPFGNSTLVITILTKMDNKFS 304

Query: 198 IESKELVRKL 200
              +E+V+ +
Sbjct: 305 RPVEEVVKNI 314

[Close]

BLAST of Carg05852 vs. ExPASy Swiss-Prot
Match: Q1PF16 (Transcription factor bHLH19 OS=Arabidopsis thaliana OX=3702 GN=BHLH19 PE=1 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 1.5e-30
Identity = 88/187 (47.06%), Postives = 114/187 (60.96%), Query Frame = 0


Query: 18  RNPPRAKDHVLAERKRREKLSQRFIALSALLPGLKKMDKASILGDAIKYIKQLQERVESL 77
           R+P  AK+HVLAERKRREKLS++FIALSALLPGLKK DK +IL DAI  +KQLQE++ +L
Sbjct: 112 RSPVLAKEHVLAERKRREKLSEKFIALSALLPGLKKADKVTILDDAISRMKQLQEQLRTL 171

Query: 78  -EEMGKNSNIESAIFIKKSHLCNNMN---DCCDDIDGEEHNHKTGFSDQKLVEIEAKISD 137
            EE      +ES I +KKS +  +      C   +  E         DQ L EIEAKIS 
Sbjct: 172 KEEKEATRQMESMILVKKSKVFFDEEPNLSCSPSVHIE--------FDQALPEIEAKISQ 231

Query: 138 KHVLVRIHCEKHEGLLAKLLEEMEKLNFKIINVNVFPFGSSTQYMVFLLQRNSDLNIE-S 197
             +L+RI CEK +G +  +L  +E    +I N  V PFG ST  +  L Q + D ++   
Sbjct: 232 NDILIRILCEKSKGCMINILNTIENFQLRIENSIVLPFGDSTLDITVLAQMDKDFSMSIL 290

Query: 198 KELVRKL 200
           K+LVR L
Sbjct: 292 KDLVRNL 290

[Close]

BLAST of Carg05852 vs. ExPASy Swiss-Prot
Match: E3SXU4 (Basic helix-loop-helix protein A OS=Pisum sativum OX=3888 GN=BHLH PE=3 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 1.2e-13
Identity = 58/158 (36.71%), Postives = 91/158 (57.59%), Query Frame = 0


Query: 17  GRNPPR---AKDHVLAERKRREKLSQRFIALSALLPGLKKMDKASILGDAIKYIKQLQER 76
           G+  P+   + +HVLAER+RREKL++RFI L +L+P + KMDKASILGD I+Y+KQL+ +
Sbjct: 457 GKGTPQDELSANHVLAERRRREKLNERFIILRSLVPFVTKMDKASILGDTIEYLKQLRRK 516

Query: 77  VESLEEMGKNSNIESAIFIKKSHLCNNMNDC-CDDIDGEEHNHKTGFSDQKLVEIEAKIS 136
           ++ LE   +N  +ES    +KS +   +       +   E N   G    K VE+ A + 
Sbjct: 517 IQDLET--RNRQMES----EKSGVTVLVGPTEKKKVRIVEGNGTGGGVRAKAVEVVASVQ 576

Query: 137 ----DKHVLVRIHCEKHEGLLAKLLEEMEKLNFKIINV 167
               +   L+ I C + EGLL  ++  + +L  ++I V
Sbjct: 577 VSIIESDALLEIECLQREGLLLDVMMMLRELRIEVIGV 608

[Close]

BLAST of Carg05852 vs. ExPASy TrEMBL
Match: A0A6J1ETR4 (transcription factor bHLH25-like OS=Cucurbita moschata OX=3662 GN=LOC111435876 PE=4 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 4.2e-105
Identity = 199/204 (97.55%), Postives = 201/204 (98.53%), Query Frame = 0


Query: 6   ASAEIWPLVNVGRNPPRAKDHVLAERKRREKLSQRFIALSALLPGLKKMDKASILGDAIK 65
           A+AEIWPLVNVGRNPPRAKDHVLAERKRREKLSQRFIALSALLPGLKKMDKASILGDAIK
Sbjct: 61  AAAEIWPLVNVGRNPPRAKDHVLAERKRREKLSQRFIALSALLPGLKKMDKASILGDAIK 120

Query: 66  YIKQLQERVESLEEMGKNSNIESAIFIKKSHLCNNMNDCCDDIDGEEHNHKTGFSDQKLV 125
           YIKQLQERVESLEEMGKNSNIESAIFIKKSHLCNNMN CCDDIDGEEHNHKTGFSDQKLV
Sbjct: 121 YIKQLQERVESLEEMGKNSNIESAIFIKKSHLCNNMNGCCDDIDGEEHNHKTGFSDQKLV 180

Query: 126 EIEAKISDKHVLVRIHCEKHEGLLAKLLEEMEKLNFKIINVNVFPFGSSTQYMVFLLQRN 185
           E+EAKISDKHVLVRIHCEKHEGLLAKLLEEMEKLNFKIIN NVFPFGSSTQYMVFLLQRN
Sbjct: 181 EMEAKISDKHVLVRIHCEKHEGLLAKLLEEMEKLNFKIINGNVFPFGSSTQYMVFLLQRN 240

Query: 186 SDLNIESKELVRKLEDELFKFITT 210
           SD NIESKELVRKLEDELFKFITT
Sbjct: 241 SDFNIESKELVRKLEDELFKFITT 264

[Close]

BLAST of Carg05852 vs. ExPASy TrEMBL
Match: A0A6J1JCQ1 (transcription factor bHLH25-like OS=Cucurbita maxima OX=3661 GN=LOC111483221 PE=4 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 2.9e-98
Identity = 191/205 (93.17%), Postives = 197/205 (96.10%), Query Frame = 0


Query: 5   AASAEIWPLVNVGRNPPRAKDHVLAERKRREKLSQRFIALSALLPGLKKMDKASILGDAI 64
           AA+AEIWPLVNVGRNPPRAKDHVLAERKRREKLSQRFIALSALLPGLKKMDKASILGDAI
Sbjct: 62  AAAAEIWPLVNVGRNPPRAKDHVLAERKRREKLSQRFIALSALLPGLKKMDKASILGDAI 121

Query: 65  KYIKQLQERVESLEEMGKNSNIESAIFIKKSHLCNNMNDCCDDIDGEEHNHKTGFSDQKL 124
           KYIKQLQERVESLEEMGKNSNIESAI IKKSHLCNNMND CDDIDGEE NHKT FSDQKL
Sbjct: 122 KYIKQLQERVESLEEMGKNSNIESAILIKKSHLCNNMND-CDDIDGEEQNHKTRFSDQKL 181

Query: 125 VEIEAKISDKHVLVRIHCEKHEGLLAKLLEEMEKLNFKIINVNVFPFGSSTQYMVFLLQR 184
           VEIEAKISDKHVLVRIHCEKHEGLLA+LLEEMEKLNF+IIN NVFPFGSSTQYMVFLLQR
Sbjct: 182 VEIEAKISDKHVLVRIHCEKHEGLLARLLEEMEKLNFRIINANVFPFGSSTQYMVFLLQR 241

Query: 185 NSDLNIESKELVRKLEDELFKFITT 210
           NS+  I+SKEL+RKLEDEL KFITT
Sbjct: 242 NSNSFIDSKELIRKLEDELVKFITT 265

[Close]

BLAST of Carg05852 vs. ExPASy TrEMBL
Match: A0A6J1C4D8 (transcription factor bHLH18-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111008381 PE=4 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 2.0e-51
Identity = 123/185 (66.49%), Postives = 142/185 (76.76%), Query Frame = 0


Query: 23  AKDHVLAERKRREKLSQRFIALSALLPGLKKMDKASILGDAIKYIKQLQERVESLEEMGK 82
           A+DHVLAERKRREKLSQRFIALSALLPGLKKMDKAS+LGDAIKYIKQ++ERV  LEE  K
Sbjct: 61  AQDHVLAERKRREKLSQRFIALSALLPGLKKMDKASVLGDAIKYIKQVEERVRVLEEKAK 120

Query: 83  NSNIESAIFIKKSHLCNNMNDCCDDIDGEEHNHKTGFSDQK-LVEIEAKISD--KHVLVR 142
             +IESAI + KSHL    NDC ++I        + FSDQK L EIEA+ISD   +VLVR
Sbjct: 121 KRSIESAILVNKSHL---YNDCDEEI--------SRFSDQKQLPEIEARISDDKNNVLVR 180

Query: 143 IHCEKHEGLLAKLLEEMEKLNFKIINVNVFPFGSSTQYMVFLLQRNSDLNIESKELVRKL 202
           IHCEK+EGL AKLL EMEK+N  I+N N FPFG+STQYMV + Q +S+L I+SKE   KL
Sbjct: 181 IHCEKNEGLFAKLLGEMEKMNLTIVNANFFPFGTSTQYMVLVAQIDSELKIKSKEGTNKL 234

Query: 203 EDELF 205
            D  F
Sbjct: 241 VDIKF 234

[Close]

BLAST of Carg05852 vs. ExPASy TrEMBL
Match: A0A6J1C590 (transcription factor bHLH18-like isoform X4 OS=Momordica charantia OX=3673 GN=LOC111008381 PE=4 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 1.7e-50
Identity = 119/175 (68.00%), Postives = 138/175 (78.86%), Query Frame = 0


Query: 23  AKDHVLAERKRREKLSQRFIALSALLPGLKKMDKASILGDAIKYIKQLQERVESLEEMGK 82
           A+DHVLAERKRREKLSQRFIALSALLPGLKKMDKAS+LGDAIKYIKQ++ERV  LEE  K
Sbjct: 61  AQDHVLAERKRREKLSQRFIALSALLPGLKKMDKASVLGDAIKYIKQVEERVRVLEEKAK 120

Query: 83  NSNIESAIFIKKSHLCNNMNDCCDDIDGEEHNHKTGFSDQK-LVEIEAKISD--KHVLVR 142
             +IESAI + KSHL    NDC ++I        + FSDQK L EIEA+ISD   +VLVR
Sbjct: 121 KRSIESAILVNKSHL---YNDCDEEI--------SRFSDQKQLPEIEARISDDKNNVLVR 180

Query: 143 IHCEKHEGLLAKLLEEMEKLNFKIINVNVFPFGSSTQYMVFLLQRNSDLNIESKE 195
           IHCEK+EGL AKLL EMEK+N  I+N N FPFG+STQYMV + Q +S+L I+SKE
Sbjct: 181 IHCEKNEGLFAKLLGEMEKMNLTIVNANFFPFGTSTQYMVLVAQIDSELKIKSKE 224

[Close]

BLAST of Carg05852 vs. ExPASy TrEMBL
Match: A0A6J1C4V4 (transcription factor bHLH18-like isoform X5 OS=Momordica charantia OX=3673 GN=LOC111008381 PE=4 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 2.8e-48
Identity = 114/166 (68.67%), Postives = 131/166 (78.92%), Query Frame = 0


Query: 23  AKDHVLAERKRREKLSQRFIALSALLPGLKKMDKASILGDAIKYIKQLQERVESLEEMGK 82
           A+DHVLAERKRREKLSQRFIALSALLPGLKKMDKAS+LGDAIKYIKQ++ERV  LEE  K
Sbjct: 61  AQDHVLAERKRREKLSQRFIALSALLPGLKKMDKASVLGDAIKYIKQVEERVRVLEEKAK 120

Query: 83  NSNIESAIFIKKSHLCNNMNDCCDDIDGEEHNHKTGFSDQK-LVEIEAKISD--KHVLVR 142
             +IESAI + KSHL    NDC ++I        + FSDQK L EIEA+ISD   +VLVR
Sbjct: 121 KRSIESAILVNKSHL---YNDCDEEI--------SRFSDQKQLPEIEARISDDKNNVLVR 180

Query: 143 IHCEKHEGLLAKLLEEMEKLNFKIINVNVFPFGSSTQYMVFLLQRN 186
           IHCEK+EGL AKLL EMEK+N  I+N N FPFG+STQYMV + Q+N
Sbjct: 181 IHCEKNEGLFAKLLGEMEKMNLTIVNANFFPFGTSTQYMVLVAQKN 215

[Close]

BLAST of Carg05852 vs. TAIR 10
Match: AT4G37850.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 161.0 bits (406), Expect = 1.0e-39
Identity = 93/190 (48.95%), Postives = 127/190 (66.84%), Query Frame = 0


Query: 18  RNPPRAKDHVLAERKRREKLSQRFIALSALLPGLKKMDKASILGDAIKYIKQLQERVESL 77
           RN   A+DH++AERKRREKL+QRF+ALSAL+PGLKKMDKAS+LGDA+K+IK LQERV  L
Sbjct: 145 RNQSNAQDHIIAERKRREKLTQRFVALSALVPGLKKMDKASVLGDALKHIKYLQERVGEL 204

Query: 78  EEMGKNSNIESAIFIKKSHLCNNMNDCCDDIDGEEHNHKTGFSDQKLVEIEAKISDKHVL 137
           EE  K   +ES + +KKS L  + N+       E+     GFSD  L EIE + SD+ VL
Sbjct: 205 EEQKKERRLESMVLVKKSKLILDDNNQSFSSSCED-----GFSDLDLPEIEVRFSDEDVL 264

Query: 138 VRIHCEKHEGLLAKLLEEMEKLNFKIINVNVFPFGSSTQYMVFLLQRNSDLNIESKELVR 197
           ++I CEK +G LAK++ E+EKL+  I N +V  FG  T  +  + ++ SD ++   ++V+
Sbjct: 265 IKILCEKQKGHLAKIMAEIEKLHILITNSSVLNFG-PTLDITIIAKKESDFDMTLMDVVK 324

Query: 198 KLEDELFKFI 208
            L   L  FI
Sbjct: 325 SLRSALSNFI 328

[Close]

BLAST of Carg05852 vs. TAIR 10
Match: AT2G22750.2 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 154.8 bits (390), Expect = 7.4e-38
Identity = 87/188 (46.28%), Postives = 124/188 (65.96%), Query Frame = 0


Query: 18  RNPPRAKDHVLAERKRREKLSQRFIALSALLPGLKKMDKASILGDAIKYIKQLQERVESL 77
           R+   A+DH+LAERKRREKL+QRF+ALSAL+PGLKKMDKAS+LGDAIK+IK LQE V+  
Sbjct: 119 RSQSNAQDHILAERKRREKLTQRFVALSALIPGLKKMDKASVLGDAIKHIKYLQESVKEY 178

Query: 78  EEMGKNSNIESAIFIKKSHLCNNMNDCCDDIDGEEHNHKTGFSDQKLVEIEAKISDKHVL 137
           EE  K   +ES + +KKS L  + N         + N  +  S   L EIE ++S K VL
Sbjct: 179 EEQKKEKTMESVVLVKKSSLVLDENHQPSSSSSSDGNRNS--SSSNLPEIEVRVSGKDVL 238

Query: 138 VRIHCEKHEGLLAKLLEEMEKLNFKIINVNVFPFGSSTQYMVFLLQRNSDLNIESKELVR 197
           ++I CEK +G + K++ E+EKL   I N NV PFG +    + + Q+N++ +++ +++V+
Sbjct: 239 IKILCEKQKGNVIKIMGEIEKLGLSITNSNVLPFGPTFDISI-IAQKNNNFDMKIEDVVK 298

Query: 198 KLEDELFK 206
            L   L K
Sbjct: 299 NLSFGLSK 303

[Close]

BLAST of Carg05852 vs. TAIR 10
Match: AT2G22750.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 154.5 bits (389), Expect = 9.7e-38
Identity = 89/188 (47.34%), Postives = 119/188 (63.30%), Query Frame = 0


Query: 18  RNPPRAKDHVLAERKRREKLSQRFIALSALLPGLKKMDKASILGDAIKYIKQLQERVESL 77
           R+   A+DH+LAERKRREKL+QRF+ALSAL+PGLKKMDKAS+LGDAIK+IK LQE V+  
Sbjct: 119 RSQSNAQDHILAERKRREKLTQRFVALSALIPGLKKMDKASVLGDAIKHIKYLQESVKEY 178

Query: 78  EEMGKNSNIESAIFIKKSHLCNNMNDCCDDIDGEEHNHKTGFSDQKLVEIEAKISDKHVL 137
           EE  K   +ES + +KKS L  + N         + N  +  S   L EIE ++S K VL
Sbjct: 179 EEQKKEKTMESVVLVKKSSLVLDENHQPSSSSSSDGNRNS--SSSNLPEIEVRVSGKDVL 238

Query: 138 VRIHCEKHEGLLAKLLEEMEKLNFKIINVNVFPFGSSTQYMVFLLQRNSDLNIESKELVR 197
           ++I CEK +G + K++ E+EKL   I N NV PFG +    +     N D+ IE  ++V+
Sbjct: 239 IKILCEKQKGNVIKIMGEIEKLGLSITNSNVLPFGPTFDISIIAQNNNFDMKIE--DVVK 298

Query: 198 KLEDELFK 206
            L   L K
Sbjct: 299 NLSFGLSK 302

[Close]

BLAST of Carg05852 vs. TAIR 10
Match: AT2G22770.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 134.8 bits (338), Expect = 8.0e-32
Identity = 76/190 (40.00%), Postives = 119/190 (62.63%), Query Frame = 0


Query: 18  RNPPRAKDHVLAERKRREKLSQRFIALSALLPGLKKMDKASILGDAIKYIKQLQERVESL 77
           R P   K+HVLAERKRR+KL++R IALSALLPGLKK DKA++L DAIK++KQLQERV+ L
Sbjct: 125 REPHLLKEHVLAERKRRQKLNERLIALSALLPGLKKTDKATVLEDAIKHLKQLQERVKKL 184

Query: 78  EE--MGKNSNIESAIFIKKSHL------CNNMNDCCDDIDGEEHNHKTGFSDQKLVEIEA 137
           EE  +      +S I +K+S +       +  + C         + +     Q +  IEA
Sbjct: 185 EEERVVTKKMDQSIILVKRSQVYLDDDSSSYSSTCSAASPLSSSSDEVSIFKQTMPMIEA 244

Query: 138 KISDKHVLVRIHCEKHEGLLAKLLEEMEKLNFKIINVNVFPFGSSTQYMVFLLQRNSDLN 197
           ++SD+ +L+R+HCEK++G + K+L  +EK   +++N    PFG+ST  +  L + ++  +
Sbjct: 245 RVSDRDLLIRVHCEKNKGCMIKILSSLEKFRLEVVNSFTLPFGNSTLVITILTKMDNKFS 304

Query: 198 IESKELVRKL 200
              +E+V+ +
Sbjct: 305 RPVEEVVKNI 314

[Close]

BLAST of Carg05852 vs. TAIR 10
Match: AT2G22760.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 134.4 bits (337), Expect = 1.0e-31
Identity = 88/187 (47.06%), Postives = 114/187 (60.96%), Query Frame = 0


Query: 18  RNPPRAKDHVLAERKRREKLSQRFIALSALLPGLKKMDKASILGDAIKYIKQLQERVESL 77
           R+P  AK+HVLAERKRREKLS++FIALSALLPGLKK DK +IL DAI  +KQLQE++ +L
Sbjct: 112 RSPVLAKEHVLAERKRREKLSEKFIALSALLPGLKKADKVTILDDAISRMKQLQEQLRTL 171

Query: 78  -EEMGKNSNIESAIFIKKSHLCNNMN---DCCDDIDGEEHNHKTGFSDQKLVEIEAKISD 137
            EE      +ES I +KKS +  +      C   +  E         DQ L EIEAKIS 
Sbjct: 172 KEEKEATRQMESMILVKKSKVFFDEEPNLSCSPSVHIE--------FDQALPEIEAKISQ 231

Query: 138 KHVLVRIHCEKHEGLLAKLLEEMEKLNFKIINVNVFPFGSSTQYMVFLLQRNSDLNIE-S 197
             +L+RI CEK +G +  +L  +E    +I N  V PFG ST  +  L Q + D ++   
Sbjct: 232 NDILIRILCEKSKGCMINILNTIENFQLRIENSIVLPFGDSTLDITVLAQMDKDFSMSIL 290

Query: 198 KELVRKL 200
           K+LVR L
Sbjct: 292 KDLVRNL 290

The following BLAST results are available for this feature:

BLAST of Carg05852 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7015418.1	4.0e-110	100.00	Transcription factor bHLH25, partial [Cucurbita argyrosperma subsp. argyrosperma...	[more]
XP_023552894.1	4.6e-106	98.05	transcription factor bHLH25-like [Cucurbita pepo subsp. pepo]	[more]
XP_022929215.1	8.6e-105	97.55	transcription factor bHLH25-like [Cucurbita moschata]	[more]
XP_022985138.1	6.0e-98	93.17	transcription factor bHLH25-like [Cucurbita maxima]	[more]
XP_022136751.1	4.2e-51	66.49	transcription factor bHLH18-like isoform X1 [Momordica charantia]	[more]

BLAST of Carg05852 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9T072	1.5e-38	48.95	Transcription factor bHLH25 OS=Arabidopsis thaliana OX=3702 GN=BHLH25 PE=2 SV=2	[more]
Q1PF17	1.0e-36	46.28	Transcription factor bHLH18 OS=Arabidopsis thaliana OX=3702 GN=BHLH18 PE=1 SV=1	[more]
Q8S3F1	1.1e-30	40.00	Transcription factor NAI1 OS=Arabidopsis thaliana OX=3702 GN=NAI1 PE=2 SV=1	[more]
Q1PF16	1.5e-30	47.06	Transcription factor bHLH19 OS=Arabidopsis thaliana OX=3702 GN=BHLH19 PE=1 SV=1	[more]
E3SXU4	1.2e-13	36.71	Basic helix-loop-helix protein A OS=Pisum sativum OX=3888 GN=BHLH PE=3 SV=1	[more]

BLAST of Carg05852 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1ETR4	4.2e-105	97.55	transcription factor bHLH25-like OS=Cucurbita moschata OX=3662 GN=LOC111435876 P...	[more]
A0A6J1JCQ1	2.9e-98	93.17	transcription factor bHLH25-like OS=Cucurbita maxima OX=3661 GN=LOC111483221 PE=...	[more]
A0A6J1C4D8	2.0e-51	66.49	transcription factor bHLH18-like isoform X1 OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A6J1C590	1.7e-50	68.00	transcription factor bHLH18-like isoform X4 OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A6J1C4V4	2.8e-48	68.67	transcription factor bHLH18-like isoform X5 OS=Momordica charantia OX=3673 GN=LO...	[more]

BLAST of Carg05852 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G37850.1	1.0e-39	48.95	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT2G22750.2	7.4e-38	46.28	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT2G22750.1	9.7e-38	47.34	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT2G22770.1	8.0e-32	40.00	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT2G22760.1	1.0e-31	47.06	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 60..87
None	No IPR available	COILS	Coil	Coil	coord: 142..162
None	No IPR available	PANTHER	PTHR45959	BHLH TRANSCRIPTION FACTOR	coord: 16..206
None	No IPR available	PANTHER	PTHR45959:SF36	TRANSCRIPTION FACTOR NAI1	coord: 16..206
None	No IPR available	CDD	cd02116	ACT	coord: 138..200 e-value: 0.00657978 score: 32.264
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	SMART	SM00353	finulus	coord: 27..76 e-value: 1.8E-16 score: 70.7
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	PFAM	PF00010	HLH	coord: 25..71 e-value: 1.1E-11 score: 44.6
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	PROSITE	PS50888	BHLH	coord: 21..70 score: 16.742168
IPR036638	Helix-loop-helix DNA-binding domain superfamily	GENE3D	4.10.280.10		coord: 14..85 e-value: 1.2E-17 score: 65.6
IPR036638	Helix-loop-helix DNA-binding domain superfamily	SUPERFAMILY	47459	HLH, helix-loop-helix DNA-binding domain	coord: 24..84
IPR002912	ACT domain	PROSITE	PS51671	ACT	coord: 137..209 score: 9.238951

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg05852-RA	Carg05852-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
cellular_component	GO:0005634	nucleus
molecular_function	GO:0046983	protein dimerization activity