Carg05845 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg05845
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description1-aminocyclopropane-1-carboxylate synthase
LocationCarg_Chr16: 4807876 .. 4809666 (+)
RNA-Seq ExpressionCarg05845
SyntenyCarg05845
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGCTGTTGTCTAGAAAAGCTACGTGCAACTCCCATGGTCAAGATTCCTCCTACTTCTTAGGCTGGGAAGAATACGAGAAGAATCCATACGACGAGATTCGAAACCCTAAAGGAATTATCCAAATGGGTCTCGCAGAAAATCAGGTTAGATACTGTTCGGTTCTTAAAATGTGAGATCTCACATTCCATCTTATTTTGGGGCCCAGTGTCCTTGACACTTTGTTACACTCTCCAATCGACCTAAGATCTCAATTAATGATTTTTTGTTGTATATTTTGATGATATAGCTGAGCTTCGACCTAGTGGAGAGGTGGCTCGAGGAGAACCCAGAGGCAGCGGGATTTAAAAGAGATGGCAAATCCATTTTTAAAGAACTTGCTCTATTTCAAGACTACAATGGCTTGCCAGCATTCAAAAAGGTACGTTTTTTATTATCAGCCTAACCCTAAGCTCATCATCATGACATGTTTTTTTTGGAGCTAATTTAATGTTTCATTTAGGCATTGGCGGAGTTCATGTCGGAAATTAGAGACAACAAAGTAACGTTCGATCCCGACCACATAGTCCTCACGGCTGGTGCTACCTCGGCTAACGAGACTCTTATGTTCTGCTTGGCTGAACCGGGCGACGCCTTCCTTCTACCTACCCCATATTACCCCGGGTACGTGTAAAAACCCCATTTGTCTTATTATGAAATTTGATTTAAGATATAAAGATATTCGTGCAATATATTTTATTGTATACCGTCGTGAAATATATTTTATATAGCTATTATATGATTATTCAATTTCATTTTTGTTCCTCATTCTTGACGTGACTTAATACAGATTTGATAGAGATTTGAAATGGAGAACGGGGATAGAGATCGTGCCCATCCAATGCTCGAGCTCGAATGGCTTCAAGATCACAAAGCCAGCATTGTTAGAAGCCTACCAAAGCGCCCAAAATCGGAACCTCAAGGTTAAAGGAGTGCTGGTCACGAACCCATCAAACCCATTAGGAACCACAATCAGCACTCAAGAACTCAACCTTCTACTCCAATTCATTGCATCAAAAAACCTTCACTTAATAAGCGACGAGATTTATTCAGGCACTGTTTTTCAAACCCCAGCCTTTGTAAGTGTCATGGAAATCCTCAACCAAAATTACAAACACAATGACCAAATCCGAAAACAAGTTCATATCGTTTATAGCCTTTCGAAAGATCTCGGCTTGCCGGGGTTTCGAGTCGGAGCCGTGTATTCAAATGACGAGGTGGTAGTAGCTGCTGTGACGAAAATGTCGAGCTTCGGGTTGGTTTCGTCGCAAACGCAGTATCTTCTATCGGCGATGTTGTCCGACAAGACGTTTAGGCGGAAGTACATAAGGGAGAATCAAATGAGGCTGAAGAAAAGACAGAGAATGCTGGTGGATGGGCTGGAAAAGGCTGGGATTAAGTGCCACAAAGGGAATGCAGGGCTATTTTCTTGGGTGGATATGAGGCATTTGTTGGGAGCTAATACGTTTGAGGCAGAAATGGAGCTTTGGAGGAAGATTGTGTTCGAGGTGCGCTTGAACGTATCGCCCGGTCAGTCGTGCCATTGCTCCGAACCCGGTTGGTTTCGTGTTTGTTTTGCCAACATGTCTGAGGCTACTTTGGATCTCGCAATGAAAAGATTCAAGGCTTTCGTGGCTGACTCCACTACTAATTCTACTTCAAACCAACTCGACACGAAGAGGAAGTCGGTGGCTAAATGGGTTTTTCGGTTATCGGTTCATGATCGCACTAGCGAATCACGAGATCGATAG

mRNA sequence

ATGGGGCTGTTGTCTAGAAAAGCTACGTGCAACTCCCATGGTCAAGATTCCTCCTACTTCTTAGGCTGGGAAGAATACGAGAAGAATCCATACGACGAGATTCGAAACCCTAAAGGAATTATCCAAATGGGTCTCGCAGAAAATCAGCTGAGCTTCGACCTAGTGGAGAGGTGGCTCGAGGAGAACCCAGAGGCAGCGGGATTTAAAAGAGATGGCAAATCCATTTTTAAAGAACTTGCTCTATTTCAAGACTACAATGGCTTGCCAGCATTCAAAAAGGCATTGGCGGAGTTCATGTCGGAAATTAGAGACAACAAAGTAACGTTCGATCCCGACCACATAGTCCTCACGGCTGGTGCTACCTCGGCTAACGAGACTCTTATGTTCTGCTTGGCTGAACCGGGCGACGCCTTCCTTCTACCTACCCCATATTACCCCGGATTTGATAGAGATTTGAAATGGAGAACGGGGATAGAGATCGTGCCCATCCAATGCTCGAGCTCGAATGGCTTCAAGATCACAAAGCCAGCATTGTTAGAAGCCTACCAAAGCGCCCAAAATCGGAACCTCAAGGTTAAAGGAGTGCTGGTCACGAACCCATCAAACCCATTAGGAACCACAATCAGCACTCAAGAACTCAACCTTCTACTCCAATTCATTGCATCAAAAAACCTTCACTTAATAAGCGACGAGATTTATTCAGGCACTGTTTTTCAAACCCCAGCCTTTGTAAGTGTCATGGAAATCCTCAACCAAAATTACAAACACAATGACCAAATCCGAAAACAAGTTCATATCGTTTATAGCCTTTCGAAAGATCTCGGCTTGCCGGGGTTTCGAGTCGGAGCCGTGTATTCAAATGACGAGGTGGTAGTAGCTGCTGTGACGAAAATGTCGAGCTTCGGGTTGGTTTCGTCGCAAACGCAGTATCTTCTATCGGCGATGTTGTCCGACAAGACGTTTAGGCGGAAGTACATAAGGGAGAATCAAATGAGGCTGAAGAAAAGACAGAGAATGCTGGTGGATGGGCTGGAAAAGGCTGGGATTAAGTGCCACAAAGGGAATGCAGGGCTATTTTCTTGGGTGGATATGAGGCATTTGTTGGGAGCTAATACGTTTGAGGCAGAAATGGAGCTTTGGAGGAAGATTGTGTTCGAGGTGCGCTTGAACGTATCGCCCGGTCAGTCGTGCCATTGCTCCGAACCCGGTTGGTTTCGTGTTTGTTTTGCCAACATGTCTGAGGCTACTTTGGATCTCGCAATGAAAAGATTCAAGGCTTTCGTGGCTGACTCCACTACTAATTCTACTTCAAACCAACTCGACACGAAGAGGAAGTCGGTGGCTAAATGGGTTTTTCGGTTATCGGTTCATGATCGCACTAGCGAATCACGAGATCGATAG

Coding sequence (CDS)

ATGGGGCTGTTGTCTAGAAAAGCTACGTGCAACTCCCATGGTCAAGATTCCTCCTACTTCTTAGGCTGGGAAGAATACGAGAAGAATCCATACGACGAGATTCGAAACCCTAAAGGAATTATCCAAATGGGTCTCGCAGAAAATCAGCTGAGCTTCGACCTAGTGGAGAGGTGGCTCGAGGAGAACCCAGAGGCAGCGGGATTTAAAAGAGATGGCAAATCCATTTTTAAAGAACTTGCTCTATTTCAAGACTACAATGGCTTGCCAGCATTCAAAAAGGCATTGGCGGAGTTCATGTCGGAAATTAGAGACAACAAAGTAACGTTCGATCCCGACCACATAGTCCTCACGGCTGGTGCTACCTCGGCTAACGAGACTCTTATGTTCTGCTTGGCTGAACCGGGCGACGCCTTCCTTCTACCTACCCCATATTACCCCGGATTTGATAGAGATTTGAAATGGAGAACGGGGATAGAGATCGTGCCCATCCAATGCTCGAGCTCGAATGGCTTCAAGATCACAAAGCCAGCATTGTTAGAAGCCTACCAAAGCGCCCAAAATCGGAACCTCAAGGTTAAAGGAGTGCTGGTCACGAACCCATCAAACCCATTAGGAACCACAATCAGCACTCAAGAACTCAACCTTCTACTCCAATTCATTGCATCAAAAAACCTTCACTTAATAAGCGACGAGATTTATTCAGGCACTGTTTTTCAAACCCCAGCCTTTGTAAGTGTCATGGAAATCCTCAACCAAAATTACAAACACAATGACCAAATCCGAAAACAAGTTCATATCGTTTATAGCCTTTCGAAAGATCTCGGCTTGCCGGGGTTTCGAGTCGGAGCCGTGTATTCAAATGACGAGGTGGTAGTAGCTGCTGTGACGAAAATGTCGAGCTTCGGGTTGGTTTCGTCGCAAACGCAGTATCTTCTATCGGCGATGTTGTCCGACAAGACGTTTAGGCGGAAGTACATAAGGGAGAATCAAATGAGGCTGAAGAAAAGACAGAGAATGCTGGTGGATGGGCTGGAAAAGGCTGGGATTAAGTGCCACAAAGGGAATGCAGGGCTATTTTCTTGGGTGGATATGAGGCATTTGTTGGGAGCTAATACGTTTGAGGCAGAAATGGAGCTTTGGAGGAAGATTGTGTTCGAGGTGCGCTTGAACGTATCGCCCGGTCAGTCGTGCCATTGCTCCGAACCCGGTTGGTTTCGTGTTTGTTTTGCCAACATGTCTGAGGCTACTTTGGATCTCGCAATGAAAAGATTCAAGGCTTTCGTGGCTGACTCCACTACTAATTCTACTTCAAACCAACTCGACACGAAGAGGAAGTCGGTGGCTAAATGGGTTTTTCGGTTATCGGTTCATGATCGCACTAGCGAATCACGAGATCGATAG

Protein sequence

MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPKGIIQMGLAENQLSFDLVERWLEENPEAAGFKRDGKSIFKELALFQDYNGLPAFKKALAEFMSEIRDNKVTFDPDHIVLTAGATSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITKPALLEAYQSAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFQTPAFVSVMEILNQNYKHNDQIRKQVHIVYSLSKDLGLPGFRVGAVYSNDEVVVAAVTKMSSFGLVSSQTQYLLSAMLSDKTFRRKYIRENQMRLKKRQRMLVDGLEKAGIKCHKGNAGLFSWVDMRHLLGANTFEAEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLAMKRFKAFVADSTTNSTSNQLDTKRKSVAKWVFRLSVHDRTSESRDR
Homology
BLAST of Carg05845 vs. NCBI nr
Match: KAG7015411.1 (1-aminocyclopropane-1-carboxylate synthase 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 940.3 bits (2429), Expect = 6.5e-270
Identity = 466/466 (100.00%), Postives = 466/466 (100.00%), Query Frame = 0

Query: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPKGIIQMGLAENQLSFDLVERWLE 60
           MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPKGIIQMGLAENQLSFDLVERWLE
Sbjct: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPKGIIQMGLAENQLSFDLVERWLE 60

Query: 61  ENPEAAGFKRDGKSIFKELALFQDYNGLPAFKKALAEFMSEIRDNKVTFDPDHIVLTAGA 120
           ENPEAAGFKRDGKSIFKELALFQDYNGLPAFKKALAEFMSEIRDNKVTFDPDHIVLTAGA
Sbjct: 61  ENPEAAGFKRDGKSIFKELALFQDYNGLPAFKKALAEFMSEIRDNKVTFDPDHIVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITKPALLE 180
           TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITKPALLE
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITKPALLE 180

Query: 181 AYQSAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFQT 240
           AYQSAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFQT
Sbjct: 181 AYQSAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFQT 240

Query: 241 PAFVSVMEILNQNYKHNDQIRKQVHIVYSLSKDLGLPGFRVGAVYSNDEVVVAAVTKMSS 300
           PAFVSVMEILNQNYKHNDQIRKQVHIVYSLSKDLGLPGFRVGAVYSNDEVVVAAVTKMSS
Sbjct: 241 PAFVSVMEILNQNYKHNDQIRKQVHIVYSLSKDLGLPGFRVGAVYSNDEVVVAAVTKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKTFRRKYIRENQMRLKKRQRMLVDGLEKAGIKCHKGNAGLFS 360
           FGLVSSQTQYLLSAMLSDKTFRRKYIRENQMRLKKRQRMLVDGLEKAGIKCHKGNAGLFS
Sbjct: 301 FGLVSSQTQYLLSAMLSDKTFRRKYIRENQMRLKKRQRMLVDGLEKAGIKCHKGNAGLFS 360

Query: 361 WVDMRHLLGANTFEAEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420
           WVDMRHLLGANTFEAEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA
Sbjct: 361 WVDMRHLLGANTFEAEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420

Query: 421 MKRFKAFVADSTTNSTSNQLDTKRKSVAKWVFRLSVHDRTSESRDR 467
           MKRFKAFVADSTTNSTSNQLDTKRKSVAKWVFRLSVHDRTSESRDR
Sbjct: 421 MKRFKAFVADSTTNSTSNQLDTKRKSVAKWVFRLSVHDRTSESRDR 466

BLAST of Carg05845 vs. NCBI nr
Match: XP_022929274.1 (1-aminocyclopropane-1-carboxylate synthase 9-like [Cucurbita moschata])

HSP 1 Score: 923.3 bits (2385), Expect = 8.2e-265
Identity = 461/479 (96.24%), Postives = 463/479 (96.66%), Query Frame = 0

Query: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPKGIIQMGLAENQLSFDLVERWLE 60
           MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPKGIIQMGLAENQL FDLVERWLE
Sbjct: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPKGIIQMGLAENQLCFDLVERWLE 60

Query: 61  ENPEAAGFKRDGKSIFKELALFQDYNGLPAFKKALAEFMSEIRDNKVTFDPDHIVLTAGA 120
           ENP+AAGFKRDGKSIFKELALFQDYNGLPAFKKALAEFMSEIRDNKVTFDPDHIVLTAGA
Sbjct: 61  ENPDAAGFKRDGKSIFKELALFQDYNGLPAFKKALAEFMSEIRDNKVTFDPDHIVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITKPALLE 180
           TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITKPALLE
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITKPALLE 180

Query: 181 AYQSAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFQT 240
           AYQSAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFI SKNLHLISDEIYSGTVFQT
Sbjct: 181 AYQSAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIVSKNLHLISDEIYSGTVFQT 240

Query: 241 PAFVSVMEILNQNYKHNDQIRKQVHIVYSLSKDLGLPGFRVGAVYSNDEVVVAAVTKMSS 300
           PAFVSVMEILNQNYKHNDQIRKQVHIVYSLSKDLGLPGFRVGAVYSNDEVVVAA TKMSS
Sbjct: 241 PAFVSVMEILNQNYKHNDQIRKQVHIVYSLSKDLGLPGFRVGAVYSNDEVVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKTFRRKYIRENQMRLKKRQRMLVDGLEKAGIKCHKGNAGLFS 360
           FGLVSSQTQYLLSAMLSD+TFRRKYIRENQMRLKKRQRMLVDGLEKAGIKCHKGNAGLFS
Sbjct: 301 FGLVSSQTQYLLSAMLSDETFRRKYIRENQMRLKKRQRMLVDGLEKAGIKCHKGNAGLFS 360

Query: 361 WVDMRHLLGANTFEAEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420
           WVDMRHLLGANTFEAEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA
Sbjct: 361 WVDMRHLLGANTFEAEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420

Query: 421 MKRFKAFVADST-------------TNSTSNQLDTKRKSVAKWVFRLSVHDRTSESRDR 467
           MKRFKAFVADST             TNSTSNQLDTKRKSVAKWVFRLSVHDRTSESRDR
Sbjct: 421 MKRFKAFVADSTTVKLNSSRIRSRNTNSTSNQLDTKRKSVAKWVFRLSVHDRTSESRDR 479

BLAST of Carg05845 vs. NCBI nr
Match: XP_023552892.1 (1-aminocyclopropane-1-carboxylate synthase 9-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 912.9 bits (2358), Expect = 1.1e-261
Identity = 455/479 (94.99%), Postives = 458/479 (95.62%), Query Frame = 0

Query: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPKGIIQMGLAENQLSFDLVERWLE 60
           MG+LSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNP GIIQMGLAENQL FDLVERWLE
Sbjct: 1   MGMLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPNGIIQMGLAENQLCFDLVERWLE 60

Query: 61  ENPEAAGFKRDGKSIFKELALFQDYNGLPAFKKALAEFMSEIRDNKVTFDPDHIVLTAGA 120
           ENP+ AGFKRDGKSIFKELALFQDYNGLPAFKKALAEFMSEIRDNKVTFDPDHIVLTAGA
Sbjct: 61  ENPDGAGFKRDGKSIFKELALFQDYNGLPAFKKALAEFMSEIRDNKVTFDPDHIVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITKPALLE 180
           TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITKPALLE
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITKPALLE 180

Query: 181 AYQSAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFQT 240
           AYQSAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFQT
Sbjct: 181 AYQSAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFQT 240

Query: 241 PAFVSVMEILNQNYKHNDQIRKQVHIVYSLSKDLGLPGFRVGAVYSNDEVVVAAVTKMSS 300
           PAFVSVMEILNQNYKHNDQIRKQVHIVYSLSKDLGLPGFRVGAVYSNDEVVVAA TKMSS
Sbjct: 241 PAFVSVMEILNQNYKHNDQIRKQVHIVYSLSKDLGLPGFRVGAVYSNDEVVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKTFRRKYIRENQMRLKKRQRMLVDGLEKAGIKCHKGNAGLFS 360
           FGLVSSQTQYLLS MLSD+TFRRKYIRENQMRLKKRQRMLVDGLEKAGIKCHKGNAGLFS
Sbjct: 301 FGLVSSQTQYLLSVMLSDETFRRKYIRENQMRLKKRQRMLVDGLEKAGIKCHKGNAGLFS 360

Query: 361 WVDMRHLLGANTFEAEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420
           WVDMRHLLGANTFEAEMELW KIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA
Sbjct: 361 WVDMRHLLGANTFEAEMELWSKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420

Query: 421 MKRFKAFVADST-------------TNSTSNQLDTKRKSVAKWVFRLSVHDRTSESRDR 467
           MKRFKAFV DST             TNST NQLDTKRKSVAKWVFRLSVHDRTSESRDR
Sbjct: 421 MKRFKAFVVDSTTIKLNSTRIRSRNTNSTLNQLDTKRKSVAKWVFRLSVHDRTSESRDR 479

BLAST of Carg05845 vs. NCBI nr
Match: XP_022985137.1 (1-aminocyclopropane-1-carboxylate synthase 9-like [Cucurbita maxima])

HSP 1 Score: 906.7 bits (2342), Expect = 8.0e-260
Identity = 452/479 (94.36%), Postives = 457/479 (95.41%), Query Frame = 0

Query: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPKGIIQMGLAENQLSFDLVERWLE 60
           MGLLS KATCNSHGQDSSYFLGWEEYEKNPY EIRNP GIIQMGLAENQL FDLVERWLE
Sbjct: 1   MGLLSTKATCNSHGQDSSYFLGWEEYEKNPYHEIRNPNGIIQMGLAENQLCFDLVERWLE 60

Query: 61  ENPEAAGFKRDGKSIFKELALFQDYNGLPAFKKALAEFMSEIRDNKVTFDPDHIVLTAGA 120
           ENP+AAGFKRDGKSIFKELALFQDYNGLPAFKKALAEFMSEIRDNKVTFDPDHIVLTAGA
Sbjct: 61  ENPDAAGFKRDGKSIFKELALFQDYNGLPAFKKALAEFMSEIRDNKVTFDPDHIVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITKPALLE 180
           TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITKPALLE
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITKPALLE 180

Query: 181 AYQSAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFQT 240
           AYQSAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFQT
Sbjct: 181 AYQSAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFQT 240

Query: 241 PAFVSVMEILNQNYKHNDQIRKQVHIVYSLSKDLGLPGFRVGAVYSNDEVVVAAVTKMSS 300
           PAFVSVMEILNQ+YKHNDQIRKQVHIVYSLSKDLGLPGFRVGAVYSNDEVVVAA TKMSS
Sbjct: 241 PAFVSVMEILNQSYKHNDQIRKQVHIVYSLSKDLGLPGFRVGAVYSNDEVVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKTFRRKYIRENQMRLKKRQRMLVDGLEKAGIKCHKGNAGLFS 360
           FGLVSSQTQYLLS MLSD+TFRRKYIRENQMRLKKRQRMLVDGLEKAGIKCHKGNAGLFS
Sbjct: 301 FGLVSSQTQYLLSVMLSDETFRRKYIRENQMRLKKRQRMLVDGLEKAGIKCHKGNAGLFS 360

Query: 361 WVDMRHLLGANTFEAEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420
           WVDMRHLLGANTFEAEMELWRKIVFEVRLNVSP QSCHCSEPGWFRVCFANMSEATLDLA
Sbjct: 361 WVDMRHLLGANTFEAEMELWRKIVFEVRLNVSPSQSCHCSEPGWFRVCFANMSEATLDLA 420

Query: 421 MKRFKAFVADSTT-------------NSTSNQLDTKRKSVAKWVFRLSVHDRTSESRDR 467
           MKRFKAFV DSTT             NSTSN LDTKRKSVAKWVFRLS+HDR+SESRDR
Sbjct: 421 MKRFKAFVVDSTTTKLNSTWIRSRNVNSTSNNLDTKRKSVAKWVFRLSIHDRSSESRDR 479

BLAST of Carg05845 vs. NCBI nr
Match: XP_038905873.1 (1-aminocyclopropane-1-carboxylate synthase 3-like [Benincasa hispida])

HSP 1 Score: 846.7 bits (2186), Expect = 9.8e-242
Identity = 423/478 (88.49%), Postives = 438/478 (91.63%), Query Frame = 0

Query: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPKGIIQMGLAENQLSFDLVERWLE 60
           MGLLSRKATCNSHGQDSSYF GWEEYEKNPYDEI NPKGIIQMGLAENQL FD+VE+WL 
Sbjct: 1   MGLLSRKATCNSHGQDSSYFSGWEEYEKNPYDEITNPKGIIQMGLAENQLCFDVVEKWLV 60

Query: 61  ENPEAAGFKRDGKSIFKELALFQDYNGLPAFKKALAEFMSEIRDNKVTFDPDHIVLTAGA 120
           ENP+AA FKRDGKSIF+ELALFQDYNGLPAFKKAL EFMSEIR NKVTFDP+HIVLTAGA
Sbjct: 61  ENPDAASFKRDGKSIFRELALFQDYNGLPAFKKALTEFMSEIRANKVTFDPNHIVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITKPALLE 180
           TSANETLMFCLAE GDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKIT+ ALLE
Sbjct: 121 TSANETLMFCLAELGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITEAALLE 180

Query: 181 AYQSAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFQT 240
           AYQ+AQN NL VKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGT+F+ 
Sbjct: 181 AYQNAQNLNLNVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTIFRN 240

Query: 241 PAFVSVMEILNQNYKHNDQIRKQVHIVYSLSKDLGLPGFRVGAVYSNDEVVVAAVTKMSS 300
           P FVS MEILNQNYKHN QIRKQVHIVYSLSKDLGLPGFRVGA+YSNDEVVVAA TKMSS
Sbjct: 241 PGFVSAMEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDEVVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKTFRRKYIRENQMRLKKRQRMLVDGLEKAGIKCHKGNAGLFS 360
           FGLVSSQTQYLLS MLSDK FRRKYI+ENQ RL KRQRMLV+GLEKAGIKCHKGNAGLF 
Sbjct: 301 FGLVSSQTQYLLSVMLSDKKFRRKYIKENQKRLAKRQRMLVNGLEKAGIKCHKGNAGLFC 360

Query: 361 WVDMRHLLGANTFEAEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420
           WVDMRHLLGANTFE+EMELW+KIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSE TLDLA
Sbjct: 361 WVDMRHLLGANTFESEMELWKKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEPTLDLA 420

Query: 421 MKRFKAFVADST------TNST------SNQLDTKRKSVAKWVFRLSVHDRTSESRDR 467
           M+R KAFV DST      TNS       SNQLDTKRKSVAKWVFRLSV DR SE RDR
Sbjct: 421 MQRLKAFVVDSTATSPCNTNSVTVKTTPSNQLDTKRKSVAKWVFRLSVQDRASEPRDR 478

BLAST of Carg05845 vs. ExPASy Swiss-Prot
Match: Q42881 (1-aminocyclopropane-1-carboxylate synthase 3 OS=Solanum lycopersicum OX=4081 GN=ACS3 PE=1 SV=1)

HSP 1 Score: 709.1 bits (1829), Expect = 3.2e-203
Identity = 344/468 (73.50%), Postives = 398/468 (85.04%), Query Frame = 0

Query: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPKGIIQMGLAENQLSFDLVERWLE 60
           M LLS KATCNSHGQDSSYFLGW+EYEKNPYDEI+NPKGIIQMGLAENQLSFDL+E WL 
Sbjct: 1   MKLLSEKATCNSHGQDSSYFLGWQEYEKNPYDEIQNPKGIIQMGLAENQLSFDLLESWLA 60

Query: 61  ENPEAAGFKRDGKSIFKELALFQDYNGLPAFKKALAEFMSEIRDNKVTFDPDHIVLTAGA 120
           +NP+AAGFKR+G+SIF+ELALFQDY+GLPAFK A+ +FMSEIR N+V+FD +++VLTAGA
Sbjct: 61  QNPDAAGFKRNGESIFRELALFQDYHGLPAFKNAMTKFMSEIRGNRVSFDSNNLVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITKPALLE 180
           TSANETLMFCLA  GDAFLLPTPYYPGFDRDLKWRTG EIVPI CSSSNGF+IT+ AL E
Sbjct: 121 TSANETLMFCLANQGDAFLLPTPYYPGFDRDLKWRTGAEIVPIHCSSSNGFRITESALEE 180

Query: 181 AYQSAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFQT 240
           AY  A+ RNLKVKGVLVTNPSNPLGTT++  EL LLL FI  K +HLISDEIYSGTVF +
Sbjct: 181 AYLDAKKRNLKVKGVLVTNPSNPLGTTLNRNELELLLTFIDEKGIHLISDEIYSGTVFNS 240

Query: 241 PAFVSVMEILNQNYKHNDQIRKQVHIVYSLSKDLGLPGFRVGAVYSNDEVVVAAVTKMSS 300
           P FVSVME+L +      ++ ++VHIVYSLSKDLGLPGFR+GA+YSNDE+VV+A TKMSS
Sbjct: 241 PGFVSVMEVLIEKNYMKTRVWERVHIVYSLSKDLGLPGFRIGAIYSNDEMVVSAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKTFRRKYIRENQMRLKKRQRMLVDGLEKAGIKCHKGNAGLFS 360
           FGLVSSQTQYLLS MLSDK F +KYI ENQ RLKKR  MLV GL+ AGI C + NAGLF 
Sbjct: 301 FGLVSSQTQYLLSCMLSDKKFTKKYISENQKRLKKRHAMLVKGLKSAGINCLESNAGLFC 360

Query: 361 WVDMRHLLGANTFEAEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420
           WVDMRHLL +N F+AEM+LW+KIV++V LN+SPG SCHC+EPGWFRVCFANMSE TLDLA
Sbjct: 361 WVDMRHLLSSNNFDAEMDLWKKIVYDVGLNISPGSSCHCTEPGWFRVCFANMSEDTLDLA 420

Query: 421 MKRFKAFVADSTTNSTSNQ------LDTKRKSVAKWVFRLSVHDRTSE 463
           M+R K FV  +  N+T++Q       ++K+KS +KWVFRLS +DR  E
Sbjct: 421 MRRIKDFVESTAPNATNHQNQQQSNANSKKKSFSKWVFRLSFNDRQRE 468

BLAST of Carg05845 vs. ExPASy Swiss-Prot
Match: Q9M2Y8 (1-aminocyclopropane-1-carboxylate synthase 9 OS=Arabidopsis thaliana OX=3702 GN=ACS9 PE=1 SV=1)

HSP 1 Score: 698.4 bits (1801), Expect = 5.7e-200
Identity = 343/470 (72.98%), Postives = 392/470 (83.40%), Query Frame = 0

Query: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPKGIIQMGLAENQLSFDLVERWLE 60
           M  LSRK T N+HGQDSSYFLGWEEYEKNPYDEI+NP GIIQMGLAENQL FDL+E WL 
Sbjct: 1   MKQLSRKVTSNAHGQDSSYFLGWEEYEKNPYDEIKNPNGIIQMGLAENQLCFDLIETWLA 60

Query: 61  ENPEAAGFKRDGKSIFKELALFQDYNGLPAFKKALAEFMSEIRDNKVTFDPDHIVLTAGA 120
           +NP+AAG K+DG+SIFKELALFQDY+GLP FKKALAEFM EIR N+VTFDP  IVL AG+
Sbjct: 61  KNPDAAGLKKDGQSIFKELALFQDYHGLPEFKKALAEFMEEIRGNRVTFDPSKIVLAAGS 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITKPALLE 180
           TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTG EIVPI CSSSNGF+IT+ AL +
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIHCSSSNGFQITESALQQ 180

Query: 181 AYQSAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFQT 240
           AYQ AQ  +LKVKGVLVTNPSNPLGT ++ +ELNLL+ FI SKN+HLISDEIYSGTVF  
Sbjct: 181 AYQQAQKLDLKVKGVLVTNPSNPLGTMLTRRELNLLVDFITSKNIHLISDEIYSGTVFGF 240

Query: 241 PAFVSVMEILNQNYKHNDQIRKQVHIVYSLSKDLGLPGFRVGAVYSNDEVVVAAVTKMSS 300
             FVSVM++L      N ++ K+VHIVYSLSKDLGLPGFRVGA+YSNDE+VV+A TKMSS
Sbjct: 241 EQFVSVMDVLKDKNLENSEVSKRVHIVYSLSKDLGLPGFRVGAIYSNDEMVVSAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKTFRRKYIRENQMRLKKRQRMLVDGLEKAGIKCHKGNAGLFS 360
           FGLVSSQTQYLLSA+LSDK F   Y+ ENQ RLK RQ+ LV GLE AGI C K NAGLF 
Sbjct: 301 FGLVSSQTQYLLSALLSDKKFTSTYLDENQKRLKIRQKKLVSGLEAAGITCLKSNAGLFC 360

Query: 361 WVDMRHLLGANTFEAEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420
           WVDMRHLL  NTFEAE+ELW+KIV++V+LN+SPG SCHC+EPGWFRVCFANMSE TLDLA
Sbjct: 361 WVDMRHLLDTNTFEAELELWKKIVYDVKLNISPGSSCHCTEPGWFRVCFANMSEDTLDLA 420

Query: 421 MKRFKAFVADS-----TTNSTSNQLDTKRK-SVAKWVFRLSVHDRTSESR 465
           MKR K +V  +      + S+ +++ + RK +V+ WVFR+S  DR  + R
Sbjct: 421 MKRLKEYVESTDSRRVISKSSHDRIKSLRKRTVSNWVFRVSWTDRVPDER 470

BLAST of Carg05845 vs. ExPASy Swiss-Prot
Match: Q9T065 (1-aminocyclopropane-1-carboxylate synthase 8 OS=Arabidopsis thaliana OX=3702 GN=ACS8 PE=1 SV=1)

HSP 1 Score: 693.0 bits (1787), Expect = 2.4e-198
Identity = 334/469 (71.22%), Postives = 390/469 (83.16%), Query Frame = 0

Query: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPKGIIQMGLAENQLSFDLVERWLE 60
           MGLLS+KA+CN+HGQDSSYF GWEEYEKNPYDEI+NP GIIQMGLAENQLSFDL+E WL 
Sbjct: 1   MGLLSKKASCNTHGQDSSYFWGWEEYEKNPYDEIKNPDGIIQMGLAENQLSFDLIESWLA 60

Query: 61  ENPEAAGFKRDGKSIFKELALFQDYNGLPAFKKALAEFMSEIRDNKVTFDPDHIVLTAGA 120
           +NP+AA F+R+G+SIF+ELALFQDY+GLP+FK A+A+FMSE R N+V+F+P+ +VLTAGA
Sbjct: 61  KNPDAANFQREGQSIFRELALFQDYHGLPSFKNAMADFMSENRGNRVSFNPNKLVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITKPALLE 180
           T ANETLMFCLA+PGDAFLLPTPYYPGFDRDLKWRTG EIVPIQC S+NGF+ITK AL E
Sbjct: 121 TPANETLMFCLADPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIQCKSANGFRITKVALEE 180

Query: 181 AYQSAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFQT 240
           AY+ AQ  NLKVKGVL+TNPSNPLGTT +  ELN LL FI+ K +HLISDEIYSGTVF  
Sbjct: 181 AYEQAQKLNLKVKGVLITNPSNPLGTTTTRTELNHLLDFISRKKIHLISDEIYSGTVFTN 240

Query: 241 PAFVSVMEILNQNYKHNDQIRKQVHIVYSLSKDLGLPGFRVGAVYSNDEVVVAAVTKMSS 300
           P F+SVME+L      N  +  +VHIVYSLSKDLGLPGFRVG +YSND+ VV+A TKMSS
Sbjct: 241 PGFISVMEVLKDRKLENTDVFDRVHIVYSLSKDLGLPGFRVGVIYSNDDFVVSAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKTFRRKYIRENQMRLKKRQRMLVDGLEKAGIKCHKGNAGLFS 360
           FGL+SSQTQYLLSA+LSDKTF + Y+ ENQ+RLK R + LV GLE AGI+C K NAGLF 
Sbjct: 301 FGLISSQTQYLLSALLSDKTFTKNYLEENQIRLKNRHKKLVSGLEAAGIECLKSNAGLFC 360

Query: 361 WVDMRHLLGANTFEAEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420
           WVDMRHLL +NTFEAE+ELW+KIV+EV+LN+SPG SCHC+EPGWFRVCFAN+SE TL +A
Sbjct: 361 WVDMRHLLKSNTFEAEIELWKKIVYEVKLNISPGSSCHCNEPGWFRVCFANLSEETLKVA 420

Query: 421 MKRFKAFVADST----TNSTSNQLDTKRK-SVAKWVFRLSVHDRTSESR 465
           + R K FV   +    + S   +L   RK  V+ WVFRLS HDR  E R
Sbjct: 421 LDRLKRFVDGPSPTRRSQSEHQRLKNLRKMKVSNWVFRLSFHDREPEER 469

BLAST of Carg05845 vs. ExPASy Swiss-Prot
Match: Q00257 (1-aminocyclopropane-1-carboxylate synthase CMA101 OS=Cucurbita maxima OX=3661 GN=ACS2 PE=2 SV=1)

HSP 1 Score: 690.6 bits (1781), Expect = 1.2e-197
Identity = 334/466 (71.67%), Postives = 389/466 (83.48%), Query Frame = 0

Query: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPKGIIQMGLAENQLSFDLVERWLE 60
           M +LS KATCNSHGQDSSYFLGWE YE NP+    NP GIIQMGLAENQLSFDL+E WL 
Sbjct: 1   MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLS 60

Query: 61  ENPEAAGFKRDGKSIFKELALFQDYNGLPAFKKALAEFMSEIRDNKVTFDPDHIVLTAGA 120
           +NP+AA FKRDGKSIF+ELALFQDY+GLPAFKKAL EFM+EIR NKV+F+ ++IVLTAGA
Sbjct: 61  KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITKPALLE 180
           TSANETLMFCLAE GDAFLLPTPYYPGFDRDLKWRTG+EIVPI C+SSNGF+IT+ AL +
Sbjct: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNGFQITQSALEQ 180

Query: 181 AYQSAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFQT 240
           AY+ AQ RNL+VKGVLVTNPSNPLGTT++  ELNL+  FI SK +HLISDEIYSGTVF +
Sbjct: 181 AYKDAQTRNLRVKGVLVTNPSNPLGTTMNRDELNLVFDFITSKGIHLISDEIYSGTVFGS 240

Query: 241 PAFVSVMEILNQNYKHNDQIRKQVHIVYSLSKDLGLPGFRVGAVYSNDEVVVAAVTKMSS 300
           P FVS ME+L +    ++++ K+VHIVYSLSKDLGLPGFRVGA+YSND++VVAA TKMSS
Sbjct: 241 PGFVSAMEVLKERSSEDEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKTFRRKYIRENQMRLKKRQRMLVDGLEKAGIKCHKGNAGLFS 360
           FGLVSSQTQYLLSAMLSDK F   YI ENQ RLK+RQ+MLV GL+KAGI C   NAGLF 
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFTISYISENQKRLKQRQKMLVSGLQKAGINCLDSNAGLFC 360

Query: 361 WVDMRHLLGANTFEAEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420
           WVDMRHLL ++ FE+E+ELW+KIV+EV LN+SPG SCHC+EPGWFRVCFANMSE+TL LA
Sbjct: 361 WVDMRHLLESDKFESELELWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKLA 420

Query: 421 MKRFKAFVADSTTNSTSNQ--------LDTKRKSVAKWVFRLSVHD 459
           ++R K+FV +  + +TSN          + K+    KWVFR S  +
Sbjct: 421 VRRLKSFVTELRSTTTSNHRNHDNKICKNIKKNIFTKWVFRQSAQE 466

BLAST of Carg05845 vs. ExPASy Swiss-Prot
Match: Q37001 (1-aminocyclopropane-1-carboxylate synthase 5 OS=Arabidopsis thaliana OX=3702 GN=ACS5 PE=1 SV=1)

HSP 1 Score: 686.4 bits (1770), Expect = 2.2e-196
Identity = 330/470 (70.21%), Postives = 386/470 (82.13%), Query Frame = 0

Query: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPKGIIQMGLAENQLSFDLVERWLE 60
           M  LS K T N HGQDSSYFLGWEEYEKNPYDEI+NP G+IQMGLAENQL FDL+E WL 
Sbjct: 1   MKQLSTKVTSNGHGQDSSYFLGWEEYEKNPYDEIKNPNGMIQMGLAENQLCFDLIESWLT 60

Query: 61  ENPEAAGFKRDGKSIFKELALFQDYNGLPAFKKALAEFMSEIRDNKVTFDPDHIVLTAGA 120
           +NP+AA  KR+G+SIF+ELALFQDY+G+P FKKA+AEFM EIR N+VTFDP  IVL AG+
Sbjct: 61  KNPDAASLKRNGQSIFRELALFQDYHGMPEFKKAMAEFMEEIRGNRVTFDPKKIVLAAGS 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITKPALLE 180
           TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTG EIVPI CSSSNGF+IT+ AL +
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIHCSSSNGFQITESALQQ 180

Query: 181 AYQSAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFQT 240
           AYQ AQ  +LKVKGVLVTNPSNPLGT ++ +ELNLL+ FI SKN+HLISDEIYSGT+F  
Sbjct: 181 AYQQAQKLDLKVKGVLVTNPSNPLGTALTRRELNLLVDFITSKNIHLISDEIYSGTMFGF 240

Query: 241 PAFVSVMEILNQNYKHNDQIRKQVHIVYSLSKDLGLPGFRVGAVYSNDEVVVAAVTKMSS 300
             F+SVM++L      + ++ K+VH+VYSLSKDLGLPGFRVGA+YSNDE++V+A TKMSS
Sbjct: 241 EQFISVMDVLKDKKLEDTEVSKRVHVVYSLSKDLGLPGFRVGAIYSNDEMIVSAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKTFRRKYIRENQMRLKKRQRMLVDGLEKAGIKCHKGNAGLFS 360
           FGLVSSQTQYLLSA+LSDK F  +Y+ ENQ RLK RQR LV GLE AGI C + NAGLF 
Sbjct: 301 FGLVSSQTQYLLSALLSDKKFTSQYLEENQKRLKSRQRRLVSGLESAGITCLRSNAGLFC 360

Query: 361 WVDMRHLLGANTFEAEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420
           WVDMRHLL  NTFEAE++LW+KIV+ V+LN+SPG SCHC+EPGWFRVCFANMSE TLDLA
Sbjct: 361 WVDMRHLLDTNTFEAELDLWKKIVYNVKLNISPGSSCHCTEPGWFRVCFANMSEDTLDLA 420

Query: 421 MKRFKAFVADS------TTNSTSNQLDTKRKSVAKWVFRLSVHDRTSESR 465
           +KR K FV  +      + +S       ++K+V+ WVFR+S  DR  + R
Sbjct: 421 LKRLKTFVESTDCGRMISRSSHERLKSLRKKTVSNWVFRVSWTDRVPDER 470

BLAST of Carg05845 vs. ExPASy TrEMBL
Match: A0A6J1ENA4 (1-aminocyclopropane-1-carboxylate synthase 9-like OS=Cucurbita moschata OX=3662 GN=LOC111435906 PE=3 SV=1)

HSP 1 Score: 923.3 bits (2385), Expect = 4.0e-265
Identity = 461/479 (96.24%), Postives = 463/479 (96.66%), Query Frame = 0

Query: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPKGIIQMGLAENQLSFDLVERWLE 60
           MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPKGIIQMGLAENQL FDLVERWLE
Sbjct: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPKGIIQMGLAENQLCFDLVERWLE 60

Query: 61  ENPEAAGFKRDGKSIFKELALFQDYNGLPAFKKALAEFMSEIRDNKVTFDPDHIVLTAGA 120
           ENP+AAGFKRDGKSIFKELALFQDYNGLPAFKKALAEFMSEIRDNKVTFDPDHIVLTAGA
Sbjct: 61  ENPDAAGFKRDGKSIFKELALFQDYNGLPAFKKALAEFMSEIRDNKVTFDPDHIVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITKPALLE 180
           TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITKPALLE
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITKPALLE 180

Query: 181 AYQSAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFQT 240
           AYQSAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFI SKNLHLISDEIYSGTVFQT
Sbjct: 181 AYQSAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIVSKNLHLISDEIYSGTVFQT 240

Query: 241 PAFVSVMEILNQNYKHNDQIRKQVHIVYSLSKDLGLPGFRVGAVYSNDEVVVAAVTKMSS 300
           PAFVSVMEILNQNYKHNDQIRKQVHIVYSLSKDLGLPGFRVGAVYSNDEVVVAA TKMSS
Sbjct: 241 PAFVSVMEILNQNYKHNDQIRKQVHIVYSLSKDLGLPGFRVGAVYSNDEVVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKTFRRKYIRENQMRLKKRQRMLVDGLEKAGIKCHKGNAGLFS 360
           FGLVSSQTQYLLSAMLSD+TFRRKYIRENQMRLKKRQRMLVDGLEKAGIKCHKGNAGLFS
Sbjct: 301 FGLVSSQTQYLLSAMLSDETFRRKYIRENQMRLKKRQRMLVDGLEKAGIKCHKGNAGLFS 360

Query: 361 WVDMRHLLGANTFEAEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420
           WVDMRHLLGANTFEAEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA
Sbjct: 361 WVDMRHLLGANTFEAEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420

Query: 421 MKRFKAFVADST-------------TNSTSNQLDTKRKSVAKWVFRLSVHDRTSESRDR 467
           MKRFKAFVADST             TNSTSNQLDTKRKSVAKWVFRLSVHDRTSESRDR
Sbjct: 421 MKRFKAFVADSTTVKLNSSRIRSRNTNSTSNQLDTKRKSVAKWVFRLSVHDRTSESRDR 479

BLAST of Carg05845 vs. ExPASy TrEMBL
Match: A0A6J1J417 (1-aminocyclopropane-1-carboxylate synthase 9-like OS=Cucurbita maxima OX=3661 GN=LOC111483220 PE=3 SV=1)

HSP 1 Score: 906.7 bits (2342), Expect = 3.9e-260
Identity = 452/479 (94.36%), Postives = 457/479 (95.41%), Query Frame = 0

Query: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPKGIIQMGLAENQLSFDLVERWLE 60
           MGLLS KATCNSHGQDSSYFLGWEEYEKNPY EIRNP GIIQMGLAENQL FDLVERWLE
Sbjct: 1   MGLLSTKATCNSHGQDSSYFLGWEEYEKNPYHEIRNPNGIIQMGLAENQLCFDLVERWLE 60

Query: 61  ENPEAAGFKRDGKSIFKELALFQDYNGLPAFKKALAEFMSEIRDNKVTFDPDHIVLTAGA 120
           ENP+AAGFKRDGKSIFKELALFQDYNGLPAFKKALAEFMSEIRDNKVTFDPDHIVLTAGA
Sbjct: 61  ENPDAAGFKRDGKSIFKELALFQDYNGLPAFKKALAEFMSEIRDNKVTFDPDHIVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITKPALLE 180
           TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITKPALLE
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITKPALLE 180

Query: 181 AYQSAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFQT 240
           AYQSAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFQT
Sbjct: 181 AYQSAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFQT 240

Query: 241 PAFVSVMEILNQNYKHNDQIRKQVHIVYSLSKDLGLPGFRVGAVYSNDEVVVAAVTKMSS 300
           PAFVSVMEILNQ+YKHNDQIRKQVHIVYSLSKDLGLPGFRVGAVYSNDEVVVAA TKMSS
Sbjct: 241 PAFVSVMEILNQSYKHNDQIRKQVHIVYSLSKDLGLPGFRVGAVYSNDEVVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKTFRRKYIRENQMRLKKRQRMLVDGLEKAGIKCHKGNAGLFS 360
           FGLVSSQTQYLLS MLSD+TFRRKYIRENQMRLKKRQRMLVDGLEKAGIKCHKGNAGLFS
Sbjct: 301 FGLVSSQTQYLLSVMLSDETFRRKYIRENQMRLKKRQRMLVDGLEKAGIKCHKGNAGLFS 360

Query: 361 WVDMRHLLGANTFEAEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420
           WVDMRHLLGANTFEAEMELWRKIVFEVRLNVSP QSCHCSEPGWFRVCFANMSEATLDLA
Sbjct: 361 WVDMRHLLGANTFEAEMELWRKIVFEVRLNVSPSQSCHCSEPGWFRVCFANMSEATLDLA 420

Query: 421 MKRFKAFVADSTT-------------NSTSNQLDTKRKSVAKWVFRLSVHDRTSESRDR 467
           MKRFKAFV DSTT             NSTSN LDTKRKSVAKWVFRLS+HDR+SESRDR
Sbjct: 421 MKRFKAFVVDSTTTKLNSTWIRSRNVNSTSNNLDTKRKSVAKWVFRLSIHDRSSESRDR 479

BLAST of Carg05845 vs. ExPASy TrEMBL
Match: A0A6J1FWL0 (1-aminocyclopropane-1-carboxylate synthase 9-like OS=Cucurbita moschata OX=3662 GN=LOC111448029 PE=3 SV=1)

HSP 1 Score: 837.8 bits (2163), Expect = 2.2e-239
Identity = 410/467 (87.79%), Postives = 438/467 (93.79%), Query Frame = 0

Query: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPKGIIQMGLAENQLSFDLVERWLE 60
           MG+LSRKATCNSHGQDSSYFLGWEEY+KNPYDEI NPKGIIQMGLAENQL FDL+ERWLE
Sbjct: 1   MGILSRKATCNSHGQDSSYFLGWEEYDKNPYDEILNPKGIIQMGLAENQLCFDLLERWLE 60

Query: 61  ENPEAAGFKRDGKSIFKELALFQDYNGLPAFKKALAEFMSEIRDNKVTFDPDHIVLTAGA 120
           ENP+AAGFKRDGKSIF+ELALFQDYNGLPAFKKALAEFMSEIR N+VTF+PDHIVLTAGA
Sbjct: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGNRVTFNPDHIVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITKPALLE 180
           TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPI CSSSNGF+IT+ ALLE
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFRITESALLE 180

Query: 181 AYQSAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFQT 240
           AYQSAQ+R+LKVKGVLVTNPSNPLGTTI+TQELNLLLQFIASKNLHLISDEIYSGTVF+ 
Sbjct: 181 AYQSAQSRDLKVKGVLVTNPSNPLGTTITTQELNLLLQFIASKNLHLISDEIYSGTVFRN 240

Query: 241 PAFVSVMEILNQNYKHNDQIRKQVHIVYSLSKDLGLPGFRVGAVYSNDEVVVAAVTKMSS 300
           P FVSVMEILNQNYKHN QIRKQVHIVYSLSKDLGLPGFRVGA+YSND+VVVAA TKMSS
Sbjct: 241 PGFVSVMEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKTFRRKYIRENQMRLKKRQRMLVDGLEKAGIKCHKGNAGLFS 360
           FGLVSSQTQYLLSAMLSDK F + YI+ENQ RL+KRQ+MLV GL+KAGIKCHKGNAGLF 
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFTKNYIKENQKRLEKRQKMLVRGLQKAGIKCHKGNAGLFC 360

Query: 361 WVDMRHLLGANTFEAEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420
           WVDMRHLLGANTFE+EMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA
Sbjct: 361 WVDMRHLLGANTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420

Query: 421 MKRFKAFVADS------TTNSTSNQLDTKRKSVAKWVFRLSVHDRTS 462
           M+R KAFV DS      TT +T+N L TKRK++ KWVFRLS +DR +
Sbjct: 421 MQRLKAFVVDSTVTTSPTTTTTTNHLPTKRKNMVKWVFRLSFNDRVA 467

BLAST of Carg05845 vs. ExPASy TrEMBL
Match: A0A6J1J434 (1-aminocyclopropane-1-carboxylate synthase 9-like OS=Cucurbita maxima OX=3661 GN=LOC111483239 PE=3 SV=1)

HSP 1 Score: 833.9 bits (2153), Expect = 3.2e-238
Identity = 409/465 (87.96%), Postives = 436/465 (93.76%), Query Frame = 0

Query: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPKGIIQMGLAENQLSFDLVERWLE 60
           MG+LSRKATCNSHGQDSSYFLGWEEY+KNPYDEI NPKGIIQMGLAENQL FDL+ERWLE
Sbjct: 1   MGILSRKATCNSHGQDSSYFLGWEEYDKNPYDEILNPKGIIQMGLAENQLCFDLLERWLE 60

Query: 61  ENPEAAGFKRDGKSIFKELALFQDYNGLPAFKKALAEFMSEIRDNKVTFDPDHIVLTAGA 120
           ENP+AAGFKRDGKSIF+ELALFQDYNGLPAFKKALAEFMSEIR N+VTF+PDHIVLTAGA
Sbjct: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGNRVTFNPDHIVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITKPALLE 180
           TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPI CSSSNGF+IT+ ALLE
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSSNGFRITESALLE 180

Query: 181 AYQSAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFQT 240
           AYQSAQ+R+LKVKGVLVTNPSNPLGTTI+TQELNLLLQFIASKNLHLISDEIYSGTVF+ 
Sbjct: 181 AYQSAQSRDLKVKGVLVTNPSNPLGTTITTQELNLLLQFIASKNLHLISDEIYSGTVFRN 240

Query: 241 PAFVSVMEILNQNYKHNDQIRKQVHIVYSLSKDLGLPGFRVGAVYSNDEVVVAAVTKMSS 300
           P FVSVMEILNQNYKHN QIRKQVHIVYSLSKDLGLPGFRVGA+YSND+VVVAA TKMSS
Sbjct: 241 PGFVSVMEILNQNYKHNHQIRKQVHIVYSLSKDLGLPGFRVGAIYSNDDVVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKTFRRKYIRENQMRLKKRQRMLVDGLEKAGIKCHKGNAGLFS 360
           FGLVSSQTQYLLSAMLSDK F + YI+ENQ RL+KRQ+MLV GL+KAGIKCHKGNAGLF 
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFTKNYIKENQKRLEKRQKMLVRGLQKAGIKCHKGNAGLFC 360

Query: 361 WVDMRHLLGANTFEAEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420
           WVDMRHLLGANTFE+EMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA
Sbjct: 361 WVDMRHLLGANTFESEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420

Query: 421 MKRFKAFVADS------TTNSTSNQLDTKRKSVAKWVFRLSVHDR 460
           M+R KAFV  S      TT +T+N L TKRK++ KWVFRLS +DR
Sbjct: 421 MQRLKAFVVYSTVATSPTTTTTTNHLPTKRKNMVKWVFRLSFNDR 465

BLAST of Carg05845 vs. ExPASy TrEMBL
Match: A0A6J1C5C9 (1-aminocyclopropane-1-carboxylate synthase CMA101-like OS=Momordica charantia OX=3673 GN=LOC111008407 PE=3 SV=1)

HSP 1 Score: 794.7 bits (2051), Expect = 2.1e-226
Identity = 391/466 (83.91%), Postives = 421/466 (90.34%), Query Frame = 0

Query: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPKGIIQMGLAENQLSFDLVERWLE 60
           MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNP GIIQMGLAENQL FDL+E+WLE
Sbjct: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPGGIIQMGLAENQLCFDLLEKWLE 60

Query: 61  ENPEAAGFKRDGKSIFKELALFQDYNGLPAFKKALAEFMSEIRDNKVTFDPDHIVLTAGA 120
           ENP+AAGFKRDGKSIF+ELALFQDYNGLPAFKKALAEFMSEIR NKV FDP+HIVLTAGA
Sbjct: 61  ENPDAAGFKRDGKSIFRELALFQDYNGLPAFKKALAEFMSEIRGNKVRFDPNHIVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITKPALLE 180
           TSANETLMFCLAEP DAFLLPTPYYPGFDRDLKWRTGIEIVPI CSS NGF+IT+ ALL+
Sbjct: 121 TSANETLMFCLAEPDDAFLLPTPYYPGFDRDLKWRTGIEIVPIHCSSDNGFRITEAALLQ 180

Query: 181 AYQSAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFQT 240
           AYQ+AQ+R+LKVKGVLVTNPSNPLGTTIS QEL+LLL+F+A+K+LHLISDEIYSGTVF +
Sbjct: 181 AYQNAQDRHLKVKGVLVTNPSNPLGTTISRQELSLLLRFVATKDLHLISDEIYSGTVFGS 240

Query: 241 PAFVSVMEILNQNYKHNDQIRKQVHIVYSLSKDLGLPGFRVGAVYSNDEVVVAAVTKMSS 300
           P FVS ME+LNQ YK++ Q+RK+VHIVYSLSKDLGLPGFRVGA+YSNDEVVVAA TKMSS
Sbjct: 241 PGFVSAMEVLNQKYKNDAQVRKRVHIVYSLSKDLGLPGFRVGAIYSNDEVVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKTFRRKYIRENQMRLKKRQRMLVDGLEKAGIKCHKGNAGLFS 360
           FGLVSSQTQYLLSAMLSD+ FR  YIRENQ RL +RQRMLV GLEK GIKCHKGNAGLF 
Sbjct: 301 FGLVSSQTQYLLSAMLSDEKFRGSYIRENQRRLLRRQRMLVSGLEKGGIKCHKGNAGLFC 360

Query: 361 WVDMRHLLGANTFEAEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420
           WVDMRHLLG  TFEAEMELWRKIVFEV LNVSPGQSCHCSEPGWFRVCFANMSEATL++A
Sbjct: 361 WVDMRHLLGEQTFEAEMELWRKIVFEVGLNVSPGQSCHCSEPGWFRVCFANMSEATLEVA 420

Query: 421 MKRFKAFVADSTTNSTSNQLDTKRKSVAKWVFRLSVHDRTSESRDR 467
           M+R K F   + TN+        RKSV KWVFRLSV DRT E R R
Sbjct: 421 MRRLKDFFVATKTNA------NVRKSVVKWVFRLSVQDRTGEPRHR 460

BLAST of Carg05845 vs. TAIR 10
Match: AT3G49700.1 (1-aminocyclopropane-1-carboxylate synthase 9 )

HSP 1 Score: 698.4 bits (1801), Expect = 4.0e-201
Identity = 343/470 (72.98%), Postives = 392/470 (83.40%), Query Frame = 0

Query: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPKGIIQMGLAENQLSFDLVERWLE 60
           M  LSRK T N+HGQDSSYFLGWEEYEKNPYDEI+NP GIIQMGLAENQL FDL+E WL 
Sbjct: 1   MKQLSRKVTSNAHGQDSSYFLGWEEYEKNPYDEIKNPNGIIQMGLAENQLCFDLIETWLA 60

Query: 61  ENPEAAGFKRDGKSIFKELALFQDYNGLPAFKKALAEFMSEIRDNKVTFDPDHIVLTAGA 120
           +NP+AAG K+DG+SIFKELALFQDY+GLP FKKALAEFM EIR N+VTFDP  IVL AG+
Sbjct: 61  KNPDAAGLKKDGQSIFKELALFQDYHGLPEFKKALAEFMEEIRGNRVTFDPSKIVLAAGS 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITKPALLE 180
           TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTG EIVPI CSSSNGF+IT+ AL +
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIHCSSSNGFQITESALQQ 180

Query: 181 AYQSAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFQT 240
           AYQ AQ  +LKVKGVLVTNPSNPLGT ++ +ELNLL+ FI SKN+HLISDEIYSGTVF  
Sbjct: 181 AYQQAQKLDLKVKGVLVTNPSNPLGTMLTRRELNLLVDFITSKNIHLISDEIYSGTVFGF 240

Query: 241 PAFVSVMEILNQNYKHNDQIRKQVHIVYSLSKDLGLPGFRVGAVYSNDEVVVAAVTKMSS 300
             FVSVM++L      N ++ K+VHIVYSLSKDLGLPGFRVGA+YSNDE+VV+A TKMSS
Sbjct: 241 EQFVSVMDVLKDKNLENSEVSKRVHIVYSLSKDLGLPGFRVGAIYSNDEMVVSAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKTFRRKYIRENQMRLKKRQRMLVDGLEKAGIKCHKGNAGLFS 360
           FGLVSSQTQYLLSA+LSDK F   Y+ ENQ RLK RQ+ LV GLE AGI C K NAGLF 
Sbjct: 301 FGLVSSQTQYLLSALLSDKKFTSTYLDENQKRLKIRQKKLVSGLEAAGITCLKSNAGLFC 360

Query: 361 WVDMRHLLGANTFEAEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420
           WVDMRHLL  NTFEAE+ELW+KIV++V+LN+SPG SCHC+EPGWFRVCFANMSE TLDLA
Sbjct: 361 WVDMRHLLDTNTFEAELELWKKIVYDVKLNISPGSSCHCTEPGWFRVCFANMSEDTLDLA 420

Query: 421 MKRFKAFVADS-----TTNSTSNQLDTKRK-SVAKWVFRLSVHDRTSESR 465
           MKR K +V  +      + S+ +++ + RK +V+ WVFR+S  DR  + R
Sbjct: 421 MKRLKEYVESTDSRRVISKSSHDRIKSLRKRTVSNWVFRVSWTDRVPDER 470

BLAST of Carg05845 vs. TAIR 10
Match: AT4G37770.1 (1-amino-cyclopropane-1-carboxylate synthase 8 )

HSP 1 Score: 693.0 bits (1787), Expect = 1.7e-199
Identity = 334/469 (71.22%), Postives = 390/469 (83.16%), Query Frame = 0

Query: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPKGIIQMGLAENQLSFDLVERWLE 60
           MGLLS+KA+CN+HGQDSSYF GWEEYEKNPYDEI+NP GIIQMGLAENQLSFDL+E WL 
Sbjct: 1   MGLLSKKASCNTHGQDSSYFWGWEEYEKNPYDEIKNPDGIIQMGLAENQLSFDLIESWLA 60

Query: 61  ENPEAAGFKRDGKSIFKELALFQDYNGLPAFKKALAEFMSEIRDNKVTFDPDHIVLTAGA 120
           +NP+AA F+R+G+SIF+ELALFQDY+GLP+FK A+A+FMSE R N+V+F+P+ +VLTAGA
Sbjct: 61  KNPDAANFQREGQSIFRELALFQDYHGLPSFKNAMADFMSENRGNRVSFNPNKLVLTAGA 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITKPALLE 180
           T ANETLMFCLA+PGDAFLLPTPYYPGFDRDLKWRTG EIVPIQC S+NGF+ITK AL E
Sbjct: 121 TPANETLMFCLADPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIQCKSANGFRITKVALEE 180

Query: 181 AYQSAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFQT 240
           AY+ AQ  NLKVKGVL+TNPSNPLGTT +  ELN LL FI+ K +HLISDEIYSGTVF  
Sbjct: 181 AYEQAQKLNLKVKGVLITNPSNPLGTTTTRTELNHLLDFISRKKIHLISDEIYSGTVFTN 240

Query: 241 PAFVSVMEILNQNYKHNDQIRKQVHIVYSLSKDLGLPGFRVGAVYSNDEVVVAAVTKMSS 300
           P F+SVME+L      N  +  +VHIVYSLSKDLGLPGFRVG +YSND+ VV+A TKMSS
Sbjct: 241 PGFISVMEVLKDRKLENTDVFDRVHIVYSLSKDLGLPGFRVGVIYSNDDFVVSAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKTFRRKYIRENQMRLKKRQRMLVDGLEKAGIKCHKGNAGLFS 360
           FGL+SSQTQYLLSA+LSDKTF + Y+ ENQ+RLK R + LV GLE AGI+C K NAGLF 
Sbjct: 301 FGLISSQTQYLLSALLSDKTFTKNYLEENQIRLKNRHKKLVSGLEAAGIECLKSNAGLFC 360

Query: 361 WVDMRHLLGANTFEAEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420
           WVDMRHLL +NTFEAE+ELW+KIV+EV+LN+SPG SCHC+EPGWFRVCFAN+SE TL +A
Sbjct: 361 WVDMRHLLKSNTFEAEIELWKKIVYEVKLNISPGSSCHCNEPGWFRVCFANLSEETLKVA 420

Query: 421 MKRFKAFVADST----TNSTSNQLDTKRK-SVAKWVFRLSVHDRTSESR 465
           + R K FV   +    + S   +L   RK  V+ WVFRLS HDR  E R
Sbjct: 421 LDRLKRFVDGPSPTRRSQSEHQRLKNLRKMKVSNWVFRLSFHDREPEER 469

BLAST of Carg05845 vs. TAIR 10
Match: AT5G65800.1 (ACC synthase 5 )

HSP 1 Score: 686.4 bits (1770), Expect = 1.6e-197
Identity = 330/470 (70.21%), Postives = 386/470 (82.13%), Query Frame = 0

Query: 1   MGLLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPKGIIQMGLAENQLSFDLVERWLE 60
           M  LS K T N HGQDSSYFLGWEEYEKNPYDEI+NP G+IQMGLAENQL FDL+E WL 
Sbjct: 1   MKQLSTKVTSNGHGQDSSYFLGWEEYEKNPYDEIKNPNGMIQMGLAENQLCFDLIESWLT 60

Query: 61  ENPEAAGFKRDGKSIFKELALFQDYNGLPAFKKALAEFMSEIRDNKVTFDPDHIVLTAGA 120
           +NP+AA  KR+G+SIF+ELALFQDY+G+P FKKA+AEFM EIR N+VTFDP  IVL AG+
Sbjct: 61  KNPDAASLKRNGQSIFRELALFQDYHGMPEFKKAMAEFMEEIRGNRVTFDPKKIVLAAGS 120

Query: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITKPALLE 180
           TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTG EIVPI CSSSNGF+IT+ AL +
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIHCSSSNGFQITESALQQ 180

Query: 181 AYQSAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFQT 240
           AYQ AQ  +LKVKGVLVTNPSNPLGT ++ +ELNLL+ FI SKN+HLISDEIYSGT+F  
Sbjct: 181 AYQQAQKLDLKVKGVLVTNPSNPLGTALTRRELNLLVDFITSKNIHLISDEIYSGTMFGF 240

Query: 241 PAFVSVMEILNQNYKHNDQIRKQVHIVYSLSKDLGLPGFRVGAVYSNDEVVVAAVTKMSS 300
             F+SVM++L      + ++ K+VH+VYSLSKDLGLPGFRVGA+YSNDE++V+A TKMSS
Sbjct: 241 EQFISVMDVLKDKKLEDTEVSKRVHVVYSLSKDLGLPGFRVGAIYSNDEMIVSAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKTFRRKYIRENQMRLKKRQRMLVDGLEKAGIKCHKGNAGLFS 360
           FGLVSSQTQYLLSA+LSDK F  +Y+ ENQ RLK RQR LV GLE AGI C + NAGLF 
Sbjct: 301 FGLVSSQTQYLLSALLSDKKFTSQYLEENQKRLKSRQRRLVSGLESAGITCLRSNAGLFC 360

Query: 361 WVDMRHLLGANTFEAEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLA 420
           WVDMRHLL  NTFEAE++LW+KIV+ V+LN+SPG SCHC+EPGWFRVCFANMSE TLDLA
Sbjct: 361 WVDMRHLLDTNTFEAELDLWKKIVYNVKLNISPGSSCHCTEPGWFRVCFANMSEDTLDLA 420

Query: 421 MKRFKAFVADS------TTNSTSNQLDTKRKSVAKWVFRLSVHDRTSESR 465
           +KR K FV  +      + +S       ++K+V+ WVFR+S  DR  + R
Sbjct: 421 LKRLKTFVESTDCGRMISRSSHERLKSLRKKTVSNWVFRVSWTDRVPDER 470

BLAST of Carg05845 vs. TAIR 10
Match: AT2G22810.1 (1-aminocyclopropane-1-carboxylate synthase 4 )

HSP 1 Score: 666.0 bits (1717), Expect = 2.2e-191
Identity = 328/471 (69.64%), Postives = 385/471 (81.74%), Query Frame = 0

Query: 4   LSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPKGIIQMGLAENQLSFDLVERWLEENP 63
           LSRKATCNSHGQ SSYFLGWEEYEKNPYD  +NP+GIIQMGLAENQL FDL+E WL +N 
Sbjct: 4   LSRKATCNSHGQVSSYFLGWEEYEKNPYDVTKNPQGIIQMGLAENQLCFDLLESWLAQNT 63

Query: 64  EAAGFKRDGKSIFKELALFQDYNGLPAFKKALAEFMSEIRDNKVTFDPDHIVLTAGATSA 123
           +AA FKRDG+S+F+ELALFQDY+GL +FK A A+FMSE R N+V+FD +++VLTAGATSA
Sbjct: 64  DAACFKRDGQSVFRELALFQDYHGLSSFKNAFADFMSENRGNRVSFDSNNLVLTAGATSA 123

Query: 124 NETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITKPALLEAYQ 183
           NETLMFCLA+PGDAFLLPTPYYPGFDRDLKWRTG+EIVPIQ SS+NGF+ITK AL EAY+
Sbjct: 124 NETLMFCLADPGDAFLLPTPYYPGFDRDLKWRTGVEIVPIQSSSTNGFRITKLALEEAYE 183

Query: 184 SAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIA-SKNLHLISDEIYSGTVFQTPA 243
            A+  +L VKG+L+TNPSNPLGTT +  ELN+L  FI  +KN+HL+SDEIYSGTVF +  
Sbjct: 184 QAKKLDLNVKGILITNPSNPLGTTTTQTELNILFDFITKNKNIHLVSDEIYSGTVFNSSE 243

Query: 244 FVSVMEILNQNYKHNDQIRKQVHIVYSLSKDLGLPGFRVGAVYSNDEVVVAAVTKMSSFG 303
           F+SVMEIL  N   N  +  +VHIV SLSKDLGLPGFRVGA+YSND+ V++A TKMSSFG
Sbjct: 244 FISVMEILKNNQLENTDVLNRVHIVCSLSKDLGLPGFRVGAIYSNDKDVISAATKMSSFG 303

Query: 304 LVSSQTQYLLSAMLSDKTFRRKYIRENQMRLKKRQRMLVDGLEKAGIKCHKGNAGLFSWV 363
           LVSSQTQYLLS++LSDK F + Y+RENQ RLK RQR LV GLE  GIKC K NAGLF WV
Sbjct: 304 LVSSQTQYLLSSLLSDKKFTKNYLRENQKRLKNRQRKLVLGLEAIGIKCLKSNAGLFCWV 363

Query: 364 DMRHLLGANTFEAEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLAMK 423
           DMR LL + TFEAEM+LW+KIV+EV+LN+SPG SCHC EPGWFRVCFANM + TL LA+K
Sbjct: 364 DMRPLLRSKTFEAEMDLWKKIVYEVKLNISPGSSCHCEEPGWFRVCFANMIDETLKLALK 423

Query: 424 RFKAFVADSTTN-----STSNQLDTKRK----SVAKWVFRLSVHDRTSESR 465
           R K  V D  ++     S S +L+  RK    +V+ WVFRLS HDR +E R
Sbjct: 424 RLKMLVDDENSSRRCQKSKSERLNGSRKKTMSNVSNWVFRLSFHDREAEER 474

BLAST of Carg05845 vs. TAIR 10
Match: AT4G08040.1 (1-aminocyclopropane-1-carboxylate synthase 11 )

HSP 1 Score: 610.5 bits (1573), Expect = 1.1e-174
Identity = 291/455 (63.96%), Postives = 365/455 (80.22%), Query Frame = 0

Query: 3   LLSRKATCNSHGQDSSYFLGWEEYEKNPYDEIRNPKGIIQMGLAENQLSFDLVERWLEEN 62
           +LS K   +SHGQDSSYFLGW+EYEKNP+ E  N  GI+QMGLAENQLSFDL+E+WLEE+
Sbjct: 1   MLSSKVVGDSHGQDSSYFLGWQEYEKNPFHESFNTSGIVQMGLAENQLSFDLIEKWLEEH 60

Query: 63  PEAAGFKRDGKSIFKELALFQDYNGLPAFKKALAEFMSEIRDNKVTFDPDHIVLTAGATS 122
           PE  G K++ +S+F++LALFQDY+GLPAFK A+A+FM +IR+NKV FD + +VLTAG+TS
Sbjct: 61  PEVLGLKKNDESVFRQLALFQDYHGLPAFKDAMAKFMGKIRENKVKFDTNKMVLTAGSTS 120

Query: 123 ANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGIEIVPIQCSSSNGFKITKPALLEAY 182
           ANETLMFCLA PGDAFL+P PYYPGFDRDLKWRTG+EIVPI C SSNG+KIT+ AL +AY
Sbjct: 121 ANETLMFCLANPGDAFLIPAPYYPGFDRDLKWRTGVEIVPIHCVSSNGYKITEDALEDAY 180

Query: 183 QSAQNRNLKVKGVLVTNPSNPLGTTISTQELNLLLQFIASKNLHLISDEIYSGTVFQTPA 242
           + A   NL VKGVL+TNPSNPLGT+ + +EL+LLL F ++K +H++SDEIYSGTVF +P 
Sbjct: 181 ERALKHNLNVKGVLITNPSNPLGTSTTREELDLLLTFTSTKKIHMVSDEIYSGTVFDSPE 240

Query: 243 FVSVMEILNQNYKHNDQIRKQVHIVYSLSKDLGLPGFRVGAVYSNDEVVVAAVTKMSSFG 302
           F SV+E+       N  +  ++H+VYSLSKDLGLPGFRVG +YSN+E VV+A TKMSSFG
Sbjct: 241 FTSVLEVAKDK---NMGLDGKIHVVYSLSKDLGLPGFRVGLIYSNNEKVVSAATKMSSFG 300

Query: 303 LVSSQTQYLLSAMLSDKTFRRKYIRENQMRLKKRQRMLVDGLEKAGIKCHKGNAGLFSWV 362
           L+SSQTQ+LL+ +LSD+ F   Y+ EN+ RL++R+  LV GL++AGI C K NAGLF WV
Sbjct: 301 LISSQTQHLLANLLSDERFTTNYLEENKKRLRERKDRLVSGLKEAGISCLKSNAGLFCWV 360

Query: 363 DMRHLLGANTFEAEMELWRKIVFEVRLNVSPGQSCHCSEPGWFRVCFANMSEATLDLAMK 422
           D+RHLL +NTFEAE  LW KIV EV LN+SPG SCHC EPGWFRVCFANMS+ T+++AM 
Sbjct: 361 DLRHLLKSNTFEAEHSLWTKIVCEVGLNISPGSSCHCDEPGWFRVCFANMSDQTMEVAMD 420

Query: 423 RFKAFVADSTTNSTSNQL-DTKRKS-VAKWVFRLS 456
           R K FV ++        + DT+R+S + KWV +LS
Sbjct: 421 RVKGFVDNNNGGKQKRTMWDTRRRSLINKWVSKLS 452

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7015411.16.5e-270100.001-aminocyclopropane-1-carboxylate synthase 3, partial [Cucurbita argyrosperma su... [more]
XP_022929274.18.2e-26596.241-aminocyclopropane-1-carboxylate synthase 9-like [Cucurbita moschata][more]
XP_023552892.11.1e-26194.991-aminocyclopropane-1-carboxylate synthase 9-like [Cucurbita pepo subsp. pepo][more]
XP_022985137.18.0e-26094.361-aminocyclopropane-1-carboxylate synthase 9-like [Cucurbita maxima][more]
XP_038905873.19.8e-24288.491-aminocyclopropane-1-carboxylate synthase 3-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q428813.2e-20373.501-aminocyclopropane-1-carboxylate synthase 3 OS=Solanum lycopersicum OX=4081 GN=... [more]
Q9M2Y85.7e-20072.981-aminocyclopropane-1-carboxylate synthase 9 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9T0652.4e-19871.221-aminocyclopropane-1-carboxylate synthase 8 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q002571.2e-19771.671-aminocyclopropane-1-carboxylate synthase CMA101 OS=Cucurbita maxima OX=3661 GN... [more]
Q370012.2e-19670.211-aminocyclopropane-1-carboxylate synthase 5 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Match NameE-valueIdentityDescription
A0A6J1ENA44.0e-26596.241-aminocyclopropane-1-carboxylate synthase 9-like OS=Cucurbita moschata OX=3662 ... [more]
A0A6J1J4173.9e-26094.361-aminocyclopropane-1-carboxylate synthase 9-like OS=Cucurbita maxima OX=3661 GN... [more]
A0A6J1FWL02.2e-23987.791-aminocyclopropane-1-carboxylate synthase 9-like OS=Cucurbita moschata OX=3662 ... [more]
A0A6J1J4343.2e-23887.961-aminocyclopropane-1-carboxylate synthase 9-like OS=Cucurbita maxima OX=3661 GN... [more]
A0A6J1C5C92.1e-22683.911-aminocyclopropane-1-carboxylate synthase CMA101-like OS=Momordica charantia OX... [more]
Match NameE-valueIdentityDescription
AT3G49700.14.0e-20172.981-aminocyclopropane-1-carboxylate synthase 9 [more]
AT4G37770.11.7e-19971.221-amino-cyclopropane-1-carboxylate synthase 8 [more]
AT5G65800.11.6e-19770.21ACC synthase 5 [more]
AT2G22810.12.2e-19169.641-aminocyclopropane-1-carboxylate synthase 4 [more]
AT4G08040.11.1e-17463.961-aminocyclopropane-1-carboxylate synthase 11 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00753ACCSYNTHASEcoord: 43..69
score: 63.39
coord: 295..318
score: 77.94
coord: 189..213
score: 65.11
coord: 135..156
score: 86.36
coord: 225..248
score: 63.05
coord: 113..133
score: 66.9
coord: 76..92
score: 67.5
coord: 263..287
score: 81.18
NoneNo IPR availablePANTHERPTHR43795BIFUNCTIONAL ASPARTATE AMINOTRANSFERASE AND GLUTAMATE/ASPARTATE-PREPHENATE AMINOTRANSFERASE-RELATEDcoord: 1..438
NoneNo IPR availablePANTHERPTHR43795:SF101-AMINOCYCLOPROPANE-1-CARBOXYLATE SYNTHASE 9coord: 1..438
NoneNo IPR availableCDDcd00609AAT_likecoord: 41..426
e-value: 4.60499E-66
score: 214.127
IPR004839Aminotransferase, class I/classIIPFAMPF00155Aminotran_1_2coord: 41..423
e-value: 3.2E-101
score: 339.1
IPR015422Pyridoxal phosphate-dependent transferase, small domainGENE3D3.90.1150.10Aspartate Aminotransferase, domain 1coord: 29..423
e-value: 2.2E-167
score: 558.7
IPR015421Pyridoxal phosphate-dependent transferase, major domainGENE3D3.40.640.10coord: 63..318
e-value: 2.2E-167
score: 558.7
IPR004838Aminotransferases, class-I, pyridoxal-phosphate-binding sitePROSITEPS00105AA_TRANSFER_CLASS_1coord: 269..282
IPR015424Pyridoxal phosphate-dependent transferaseSUPERFAMILY53383PLP-dependent transferasescoord: 8..428

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg05845-RACarg05845-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042218 1-aminocyclopropane-1-carboxylate biosynthetic process
biological_process GO:0009058 biosynthetic process
molecular_function GO:0016847 1-aminocyclopropane-1-carboxylate synthase activity
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0008483 transaminase activity
molecular_function GO:0003824 catalytic activity