Carg05790 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg05790
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	DNA-directed RNA polymerases II and V subunit 8A-like
Location	Carg_Chr16: 4495400 .. 4498375 (+)
RNA-Seq Expression	Carg05790
Synteny	Carg05790

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TATTTTAAATTGTGGGCTCTGAATCTTAAAAATACAAATAAATAAAATAACGAGTAAACCCTAAACCTTTGCGTGCTCCACCTCCTCCTCCTTCGCGCCGGCTTCACCTTCCTTTTCAGTAGCTCAGTGCCGCCGACGTCTTTCCTCCGCTCGTTCCGGCGCCGCCTCCGTCATAGCTGTTTGCATTGATTGTTTACTCTCTTGTTGTTATTGGTATTTGCCCAATCTCCATCTACGTTCTATTCTCACTTGAAAAACTTAAAATTAGGAATTGTCTAATGAAAGAATGGTGCCATTGATTTTTAATGCAAGTTCGCACAATTTCTGGATAGTAGTCTTGCGGCAAATTGTGAATTTTTGGACTGCCGTGTGCTCTGTCTTTTGATCTATTGATTTATTGCGTATCTGTTAATGAAGGCTTTGATCATCTGTGTTCTTTGGTTCTCTGAAAATTGCTTAGTTTCTTGTCAACATGAATTTTCCTGTATCTCGAATAAAAAACAAAGTCGCAGATATTAGAATGTGAATAGACTCAGCCACCATACACCTTTATGGCGGGTTGTTTCGATTTTACCCTTTTCGTTCTTACCGTTTTTTGAGTTTCAATAGAATCTGGTCTTGGACAGACTTTGAAGTTCTTGTATTGGTTGTTTTTGGGGGTTTAAATTTTTGTTTAGCTCTTATGATGTTTATGATGTAATTTTGCGTTTCCGCCAAGTGAGTTCTTTGATTCCGAATTCAAAATGTTTCTCCATCCCCTCTCAATTTCTACGCTTATTCTGTGCAAATTTTAGTTGGTGATTTCTTTCACTGGCTTTTCATCCCATATTTAATATTTGTTGCATGCCCACCAACTCAGATCTGCATTTTGAGGAGTGAATTCCCCATAAACATGGCGGAGCTTCTATTTAATGATGTTTTCAAGGTCAAGTGTGTCAACCCAGATGGTAAAAAGTATGATAAAGGTACTTTCCTCAAGCTTGTTATTACTTTCTCATCCTTCAATCATAATTTTCTGGTCTTTGAACTTTCTTTGAGTCTCCTTTGTAGACTTGAAACTTCGTCACCGTCTTAAGTTTGTTGTGATTGCATAAAATTGTTCTTTTAGTTTCGAGAATCGAAGCACGGAGTGAGAAGCTTTCCATGTATATGCTGCTGGATGTAAATACAGAGATATATCCAATTGATGAAGGTGAGAAGATTCTGATGGTCTTGTCTCCCACGCTGAACTATGATGGAGCTCCTGTTACCAGCTATAGTGGCCAGGTAATTTCCACCCCCCATATTCTATTGAATTCATTGCTGGAGCTCTTGGGTCATTCGTTTCATGTTCTTCATGTCTGTTTTGTCACAAGTAGAATGTTAGATGTATCTTCAAAGTTCTTAATAACTCCATAAGCTTTCTCTGATACTAAAAATTTGGAGAAGGATTTTCCCTAGCAGTCTAAAAAAGCCTAGAAAAAAGGGTCCCCGAACTTCCAGGCTAGTATCAATTGGTGCTGTTCTGTTAAGGCTGTGCTAGTGCTAGCCATGTCGCTTGAAAAGTAACACGAAAAGATGAATGAGCTCTTATTTAGGCGAGTTAAGACTCGTGTCCAAGGCTTGTGACACATGCCATAGATTAGTCAAGTATTGAAGATGCCTGCCTAGGTGTTCCAACTTTCCTTAAAGACACTTAAGAGTGTTAGGAACCACGACCCTTCACAATGATATGATTTTGTCCATTTTGAGCCTAAGATCTCATGGCTTTGCTTTTGATTTCTCCAAAAGGCCTCATACTAATGGAGTCACTGTTTTTTACTTATAAACTCATGATCAACCCTTAATTAACCGATGGAACTCCTCTTCCAACAATCCTCAACAATCCTCCCCTTGAACAAATTACACCATAGAGCCTCCCTAGAGGCCTATGAAGCCCTCGAACAACCTCTCCTTAATTGAGGCTCAACTCCTTTTTTTGGAGCCCTCGAACCAAGTACACCCTTTGTTCAACACTTGAGTAACTTTTGACTACACCTTCGAGGCTTACAACTTCTTTGTTCGACATTTGAGAATTTTATTGACATGGTTAAGTTAAGGGCATGGCTCTGATAATCCCATGTCAGGAACCACGACCCCTCAACAATGGTATGATAACATCTACTTTGAGCATAAGCTCTCGTGGCTTTGCTTTTGATTTCTCCAAAAGGTCTAATAATATCAATAGAGATAGTGTTCCTTACTTATAAACCCATGATCAACCCCTTAATTAGACGATGAGACTCCTCTCCCAACCATCTTCAGCCAGGGTCACTTTGACCAACCTGTTTTACTTCAGCCGTCAAGATTGTGAAACATAGATGAATTTTTTTTTTTTTTGCAGGAAGGGAAGAAATCGCTAGCAGATAAGTTTGAGTATATCATGCATGGTAAAATATACAAACTTTCAGATGAAGGCTCGGGTTCAGACTTGAAAGTGTAAGTAAACTTACTTACATTTCCTTGATTTACTGTTCCTAGCATAAGTAGCTACCTAGCAATCGTGTCATATAAAGCGTTCACAACACTGATGGGTTAGCTGCAATGGTTGTTGTATTTACTGTTCCTAGCATAAGTAGCTACCTAGCAATCGTGTCGTATAAAGCGTTCACAACGCTGATGATTTAGCTGCAATGGTTGTTGTATTTGCAGGGAGGTTTATGCATCTTTTGGAGGACTCCAAATGCTGTTGAAGGGAGATCCCATCCACTGTGCCAAATTCAGGGTGGATCAGAACATGTTTTTGCTCCTCCGAAAACTCTAAAAATCTCTCCTACTTGCTGTTTCACTCTCATGATGGCCTTTTGTGTGATGACTCTGTCTAGTTATTGATAATATTTGCAGATAATTTATGCGTTCAAAACTTTTACTAAATTGATCTTCATAGTCAATATGGCTTTACATAACACCAAGTGTTTTCCTAACGTTATGTTCAGTTTCTATTAAAAATTATTTAAT

mRNA sequence

TATTTTAAATTGTGGGCTCTGAATCTTAAAAATACAAATAAATAAAATAACGAGTAAACCCTAAACCTTTGCGTGCTCCACCTCCTCCTCCTTCGCGCCGGCTTCACCTTCCTTTTCAGTAGCTCAGTGCCGCCGACGTCTTTCCTCCGCTCGTTCCGGCGCCGCCTCCGTCATAGCTGTTTGCATTGATTGTTTACTCTCTTGTTGTTATTGGTATTTGCCCAATCTCCATCTACGTTCTATTCTCACTTGAAAAACTTAAAATTAGGAATTGTCTAATGAAAGAATGGTGCCATTGATTTTTAATGCAAGTTCGCACAATTTCTGGATAGTAGTCTTGCGGCAAATTGTGAATTTTTGGACTGCCGTGTGCTCTGTCTTTTGATCTATTGATTTATTGCGTATCTGTTAATGAAGGCTTTGATCATCTGTGTTCTTTGGTTCTCTGAAAATTGCTTAGTTTCTTGTCAACATGAATTTTCCTGTATCTCGAATAAAAAACAAAGTCGCAGATATTAGAATGTGAATAGACTCAGCCACCATACACCTTTATGGCGGGTTGTTTCGATTTTACCCTTTTCGTTCTTACCGTTTTTTGAGTTTCAATAGAATCTGGTCTTGGACAGACTTTGAAGTTCTTGTATTGGTTGTTTTTGGGGGTTTAAATTTTTGTTTAGCTCTTATGATGTTTATGATGTAATTTTGCGTTTCCGCCAAGTGAGTTCTTTGATTCCGAATTCAAAATGTTTCTCCATCCCCTCTCAATTTCTACGCTTATTCTGTGCAAATTTTAGTTGGTGATTTCTTTCACTGGCTTTTCATCCCATATTTAATATTTGTTGCATGCCCACCAACTCAGATCTGCATTTTGAGGAGTGAATTCCCCATAAACATGGCGGAGCTTCTATTTAATGATGTTTTCAAGGTCAAGTGTGTCAACCCAGATGGTAAAAAGTATGATAAAGTTTCGAGAATCGAAGCACGGAGTGAGAAGCTTTCCATGTATATGCTGCTGGATGTAAATACAGAGATATATCCAATTGATGAAGGTGAGAAGATTCTGATGGTCTTGTCTCCCACGCTGAACTATGATGGAGCTCCTGTTACCAGCTATAGTGGCCAGGAAGGGAAGAAATCGCTAGCAGATAAGTTTGAGTATATCATGCATGGTAAAATATACAAACTTTCAGATGAAGGCTCGGGTTCAGACTTGAAAGTGGAGGTTTATGCATCTTTTGGAGGACTCCAAATGCTGTTGAAGGGAGATCCCATCCACTGTGCCAAATTCAGGGTGGATCAGAACATGTTTTTGCTCCTCCGAAAACTCTAAAAATCTCTCCTACTTGCTGTTTCACTCTCATGATGGCCTTTTGTGTGATGACTCTGTCTAGTTATTGATAATATTTGCAGATAATTTATGCGTTCAAAACTTTTACTAAATTGATCTTCATAGTCAATATGGCTTTACATAACACCAAGTGTTTTCCTAACGTTATGTTCAGTTTCTATTAAAAATTATTTAAT

Coding sequence (CDS)

ATGGCGGAGCTTCTATTTAATGATGTTTTCAAGGTCAAGTGTGTCAACCCAGATGGTAAAAAGTATGATAAAGTTTCGAGAATCGAAGCACGGAGTGAGAAGCTTTCCATGTATATGCTGCTGGATGTAAATACAGAGATATATCCAATTGATGAAGGTGAGAAGATTCTGATGGTCTTGTCTCCCACGCTGAACTATGATGGAGCTCCTGTTACCAGCTATAGTGGCCAGGAAGGGAAGAAATCGCTAGCAGATAAGTTTGAGTATATCATGCATGGTAAAATATACAAACTTTCAGATGAAGGCTCGGGTTCAGACTTGAAAGTGGAGGTTTATGCATCTTTTGGAGGACTCCAAATGCTGTTGAAGGGAGATCCCATCCACTGTGCCAAATTCAGGGTGGATCAGAACATGTTTTTGCTCCTCCGAAAACTCTAA

Protein sequence

MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVLSPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQMLLKGDPIHCAKFRVDQNMFLLLRKL

Homology

[Close]

BLAST of Carg05790 vs. NCBI nr
Match: XP_022929200.1 (DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata] >XP_022929202.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata] >XP_022985172.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_023551974.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita pepo subsp. pepo] >KAG6577274.1 DNA-directed RNA polymerases II, IV and V subunit 8B, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015358.1 DNA-directed RNA polymerases II, IV and V subunit 8B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 295.0 bits (754), Expect = 3.4e-76
Identity = 145/145 (100.00%), Postives = 145/145 (100.00%), Query Frame = 0


Query: 1   MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60
           MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL
Sbjct: 1   MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQM 120

Query: 121 LLKGDPIHCAKFRVDQNMFLLLRKL 146
           LLKGDPIHCAKFRVDQNMFLLLRKL
Sbjct: 121 LLKGDPIHCAKFRVDQNMFLLLRKL 145

[Close]

BLAST of Carg05790 vs. NCBI nr
Match: XP_022983196.1 (DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_022983203.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_022983212.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_022983221.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP_022983229.1 DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima])

HSP 1 Score: 287.0 bits (733), Expect = 9.3e-74
Identity = 137/145 (94.48%), Postives = 145/145 (100.00%), Query Frame = 0


Query: 1   MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60
           MAE+LF+D+FKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEG+KILMVL
Sbjct: 1   MAEVLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGK+YKL+DEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120

Query: 121 LLKGDPIHCAKFRVDQNMFLLLRKL 146
           +LKGDP+HCAKFRVDQNMFLLLRKL
Sbjct: 121 MLKGDPLHCAKFRVDQNMFLLLRKL 145

[Close]

BLAST of Carg05790 vs. NCBI nr
Match: XP_038901286.1 (DNA-directed RNA polymerases II, IV and V subunit 8B-like [Benincasa hispida])

HSP 1 Score: 286.6 bits (732), Expect = 1.2e-73
Identity = 139/144 (96.53%), Postives = 143/144 (99.31%), Query Frame = 0


Query: 1   MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60
           MAELLF+D+F VKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL
Sbjct: 1   MAELLFDDIFMVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVTSY+GQEGKKSLADKFEYIMHGKIYKLSD+GSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYTGQEGKKSLADKFEYIMHGKIYKLSDDGSGSDLKVEVYASFGGLQM 120

Query: 121 LLKGDPIHCAKFRVDQNMFLLLRK 145
           LLKGDPIHCAKFRVDQNMFLLLRK
Sbjct: 121 LLKGDPIHCAKFRVDQNMFLLLRK 144

[Close]

BLAST of Carg05790 vs. NCBI nr
Match: KAG6600626.1 (DNA-directed RNA polymerases II, IV and V subunit 8B, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031262.1 DNA-directed RNA polymerases II, IV and V subunit 8B, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 285.0 bits (728), Expect = 3.5e-73
Identity = 136/145 (93.79%), Postives = 144/145 (99.31%), Query Frame = 0


Query: 1   MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60
           MAELLF+D+FKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEG+KILMVL
Sbjct: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVT YSGQ+GKKSLADKFEYIMHGK+YKL+DEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTGYSGQKGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120

Query: 121 LLKGDPIHCAKFRVDQNMFLLLRKL 146
           +LKGDP+HCAKFRVDQNMFLLLRKL
Sbjct: 121 MLKGDPLHCAKFRVDQNMFLLLRKL 145

[Close]

BLAST of Carg05790 vs. NCBI nr
Match: XP_022942165.1 (DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata])

HSP 1 Score: 283.5 bits (724), Expect = 1.0e-72
Identity = 136/145 (93.79%), Postives = 143/145 (98.62%), Query Frame = 0


Query: 1   MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60
           MAELLF+D+FKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEG+KILMVL
Sbjct: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMH K+YKL+DEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHRKLYKLADEGSGSDLKVEVYASFGGLQM 120

Query: 121 LLKGDPIHCAKFRVDQNMFLLLRKL 146
           +LKGDP+HC KFRVDQNMFLLLRKL
Sbjct: 121 MLKGDPLHCVKFRVDQNMFLLLRKL 145

[Close]

BLAST of Carg05790 vs. ExPASy Swiss-Prot
Match: Q9M1A8 (DNA-directed RNA polymerases II, IV and V subunit 8B OS=Arabidopsis thaliana OX=3702 GN=NRPB8B PE=1 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 8.8e-43
Identity = 81/142 (57.04%), Postives = 106/142 (74.65%), Query Frame = 0


Query: 4   LLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVLSPT 63
           ++F D+F V  ++PDGKK+DKV+R+EARS  L M+M LDVNTE+YP+  G+K  + ++PT
Sbjct: 6   IMFEDIFVVDKLDPDGKKFDKVTRVEARSHNLEMFMHLDVNTEVYPLAVGDKFTLAMAPT 65

Query: 64  LNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQMLLK 123
           LN DG P T Y     KK+LADK+EYIMHGK+YK+S E  G   K E+Y SFGGL MLL+
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKIS-ERDGKTPKAELYVSFGGLLMLLQ 125

Query: 124 GDPIHCAKFRVDQNMFLLLRKL 146
           GDP H + F +DQ +FLL+RKL
Sbjct: 126 GDPAHISHFELDQRLFLLMRKL 146

[Close]

BLAST of Carg05790 vs. ExPASy Swiss-Prot
Match: O81097 (DNA-directed RNA polymerases II and V subunit 8A OS=Arabidopsis thaliana OX=3702 GN=NRPB8A PE=1 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 1.2e-42
Identity = 82/142 (57.75%), Postives = 105/142 (73.94%), Query Frame = 0


Query: 4   LLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVLSPT 63
           +LF D+F V  ++PDGKK+DKV+R++A S  L M+M LDVNTE+YP+  G+K  + L+PT
Sbjct: 6   ILFEDIFVVDQLDPDGKKFDKVTRVQATSHNLEMFMHLDVNTEVYPLAVGDKFTLALAPT 65

Query: 64  LNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQMLLK 123
           LN DG P T Y     KK+LADK+EYIMHGK+YK+S E  G   K E+Y SFGGL MLLK
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKIS-ERDGKTPKAELYVSFGGLLMLLK 125

Query: 124 GDPIHCAKFRVDQNMFLLLRKL 146
           GDP H + F +DQ +FLL+RKL
Sbjct: 126 GDPAHISHFELDQRLFLLMRKL 146

[Close]

BLAST of Carg05790 vs. ExPASy Swiss-Prot
Match: Q19826 (Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhabditis elegans OX=6239 GN=rpb-8 PE=1 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 5.9e-31
Identity = 62/145 (42.76%), Postives = 100/145 (68.97%), Query Frame = 0


Query: 1   MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60
           MA ++F+D+FKVK V+PDGKK+D+VSR    +E   M +++D+N++IYP+ + +K+ +VL
Sbjct: 1   MAGIIFDDMFKVKSVDPDGKKFDRVSRYFCDAESFKMELIIDINSQIYPLKQNDKVRLVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSD-LKVEVYASFGGLQ 120
           + TL  DG         + +     ++EY+M+GK+Y+L D+ +G+D  K+  YASFGGL 
Sbjct: 61  ATTLREDGLADEGEYDPKAEYPRIKQYEYVMYGKVYRLEDDDTGTDGGKLAAYASFGGLL 120

Query: 121 MLLKGDPIHCAKFRVDQNMFLLLRK 145
           M LKG+ I+   F VD N++LL++K
Sbjct: 121 MRLKGEAINLHGFEVDMNLYLLMKK 145

[Close]

BLAST of Carg05790 vs. ExPASy Swiss-Prot
Match: A8XT81 (Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhabditis briggsae OX=6238 GN=rpb-8 PE=3 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 6.6e-30
Identity = 61/145 (42.07%), Postives = 98/145 (67.59%), Query Frame = 0


Query: 1   MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60
           MA ++F+D+F VK V+PDGKK+D+VSR    +E   M +++D+N++IYP+ + +K  +VL
Sbjct: 1   MAGIIFDDMFSVKSVDPDGKKFDRVSRYFCDAESFKMELIIDINSQIYPLKQNDKFRLVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSD-LKVEVYASFGGLQ 120
           + TL  DG         + +     ++EY+M+GK+Y+L D+ SG+D  ++  YASFGGL 
Sbjct: 61  ATTLREDGLADEGEYDPKAEYPRIKQYEYVMYGKVYRLEDDDSGTDGGQLAAYASFGGLL 120

Query: 121 MLLKGDPIHCAKFRVDQNMFLLLRK 145
           M LKG+ I+   F VD N++LL++K
Sbjct: 121 MRLKGEAINLHGFEVDMNLYLLMKK 145

[Close]

BLAST of Carg05790 vs. ExPASy Swiss-Prot
Match: Q54YW8 (DNA-directed RNA polymerases I, II, and III subunit rpabc3 OS=Dictyostelium discoideum OX=44689 GN=polr2h PE=3 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 6.6e-30
Identity = 67/144 (46.53%), Postives = 95/144 (65.97%), Query Frame = 0


Query: 1   MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60
           M  +LF D F+VK ++PDGKK+D+VSR    SE   M + +D+ T IYP+   E+  MVL
Sbjct: 1   MTSILFEDTFEVKEIDPDGKKFDRVSRFVCYSENYEMDLQIDIATHIYPLGH-ERFKMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQM 120
           + +LN DG+   S S    K SLADK++Y+M+GKI+K   E S S  KV V+ASFGGL +
Sbjct: 61  ASSLNLDGSSDNSGSYDTNKPSLADKYDYVMYGKIFKYQKENSSS--KVSVFASFGGLLV 120

Query: 121 LLKGDPIHCAKFRVDQNMFLLLRK 145
           LL+GDP +     +D  ++LL++K
Sbjct: 121 LLQGDPRYLPGIELDARIYLLIKK 141

[Close]

BLAST of Carg05790 vs. ExPASy TrEMBL
Match: A0A6J1ETP8 (DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita moschata OX=3662 GN=LOC111435865 PE=3 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 1.7e-76
Identity = 145/145 (100.00%), Postives = 145/145 (100.00%), Query Frame = 0


Query: 1   MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60
           MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL
Sbjct: 1   MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQM 120

Query: 121 LLKGDPIHCAKFRVDQNMFLLLRKL 146
           LLKGDPIHCAKFRVDQNMFLLLRKL
Sbjct: 121 LLKGDPIHCAKFRVDQNMFLLLRKL 145

[Close]

BLAST of Carg05790 vs. ExPASy TrEMBL
Match: A0A6J1J454 (DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita maxima OX=3661 GN=LOC111483257 PE=3 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 1.7e-76
Identity = 145/145 (100.00%), Postives = 145/145 (100.00%), Query Frame = 0


Query: 1   MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60
           MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL
Sbjct: 1   MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQM 120

Query: 121 LLKGDPIHCAKFRVDQNMFLLLRKL 146
           LLKGDPIHCAKFRVDQNMFLLLRKL
Sbjct: 121 LLKGDPIHCAKFRVDQNMFLLLRKL 145

[Close]

BLAST of Carg05790 vs. ExPASy TrEMBL
Match: A0A6J1J6Q9 (DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita maxima OX=3661 GN=LOC111481850 PE=3 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 4.5e-74
Identity = 137/145 (94.48%), Postives = 145/145 (100.00%), Query Frame = 0


Query: 1   MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60
           MAE+LF+D+FKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEG+KILMVL
Sbjct: 1   MAEVLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGK+YKL+DEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKLYKLADEGSGSDLKVEVYASFGGLQM 120

Query: 121 LLKGDPIHCAKFRVDQNMFLLLRKL 146
           +LKGDP+HCAKFRVDQNMFLLLRKL
Sbjct: 121 MLKGDPLHCAKFRVDQNMFLLLRKL 145

[Close]

BLAST of Carg05790 vs. ExPASy TrEMBL
Match: A0A6J1FVR2 (DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita moschata OX=3662 GN=LOC111447307 PE=3 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 5.0e-73
Identity = 136/145 (93.79%), Postives = 143/145 (98.62%), Query Frame = 0


Query: 1   MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60
           MAELLF+D+FKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEG+KILMVL
Sbjct: 1   MAELLFDDIFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGDKILMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMH K+YKL+DEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHRKLYKLADEGSGSDLKVEVYASFGGLQM 120

Query: 121 LLKGDPIHCAKFRVDQNMFLLLRKL 146
           +LKGDP+HC KFRVDQNMFLLLRKL
Sbjct: 121 MLKGDPLHCVKFRVDQNMFLLLRKL 145

[Close]

BLAST of Carg05790 vs. ExPASy TrEMBL
Match: A0A0A0KZC2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G031020 PE=3 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 1.5e-72
Identity = 137/145 (94.48%), Postives = 141/145 (97.24%), Query Frame = 0


Query: 1   MAELLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVL 60
           MA LLFND+FKVKCVNPDGKKYDKVSRIEARSEKLSM+MLLDVNTEIYPIDEGEK+LMVL
Sbjct: 1   MAGLLFNDIFKVKCVNPDGKKYDKVSRIEARSEKLSMHMLLDVNTEIYPIDEGEKLLMVL 60

Query: 61  SPTLNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQM 120
           SPTLNYDG+PVTSY  QEGKKSLADKFEYIMHGK YKLSDEGSGSDLKVEVYASFGGLQM
Sbjct: 61  SPTLNYDGSPVTSYKDQEGKKSLADKFEYIMHGKTYKLSDEGSGSDLKVEVYASFGGLQM 120

Query: 121 LLKGDPIHCAKFRVDQNMFLLLRKL 146
           LLKGDPIHCAKFRVDQNMFLLLRKL
Sbjct: 121 LLKGDPIHCAKFRVDQNMFLLLRKL 145

[Close]

BLAST of Carg05790 vs. TAIR 10
Match: AT3G59600.1 (RNA polymerase Rpb8 )

HSP 1 Score: 174.5 bits (441), Expect = 6.3e-44
Identity = 81/142 (57.04%), Postives = 106/142 (74.65%), Query Frame = 0


Query: 4   LLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVLSPT 63
           ++F D+F V  ++PDGKK+DKV+R+EARS  L M+M LDVNTE+YP+  G+K  + ++PT
Sbjct: 6   IMFEDIFVVDKLDPDGKKFDKVTRVEARSHNLEMFMHLDVNTEVYPLAVGDKFTLAMAPT 65

Query: 64  LNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQMLLK 123
           LN DG P T Y     KK+LADK+EYIMHGK+YK+S E  G   K E+Y SFGGL MLL+
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKIS-ERDGKTPKAELYVSFGGLLMLLQ 125

Query: 124 GDPIHCAKFRVDQNMFLLLRKL 146
           GDP H + F +DQ +FLL+RKL
Sbjct: 126 GDPAHISHFELDQRLFLLMRKL 146

[Close]

BLAST of Carg05790 vs. TAIR 10
Match: AT1G54250.1 (RNA polymerase Rpb8 )

HSP 1 Score: 174.1 bits (440), Expect = 8.2e-44
Identity = 82/142 (57.75%), Postives = 105/142 (73.94%), Query Frame = 0


Query: 4   LLFNDVFKVKCVNPDGKKYDKVSRIEARSEKLSMYMLLDVNTEIYPIDEGEKILMVLSPT 63
           +LF D+F V  ++PDGKK+DKV+R++A S  L M+M LDVNTE+YP+  G+K  + L+PT
Sbjct: 6   ILFEDIFVVDQLDPDGKKFDKVTRVQATSHNLEMFMHLDVNTEVYPLAVGDKFTLALAPT 65

Query: 64  LNYDGAPVTSYSGQEGKKSLADKFEYIMHGKIYKLSDEGSGSDLKVEVYASFGGLQMLLK 123
           LN DG P T Y     KK+LADK+EYIMHGK+YK+S E  G   K E+Y SFGGL MLLK
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKIS-ERDGKTPKAELYVSFGGLLMLLK 125

Query: 124 GDPIHCAKFRVDQNMFLLLRKL 146
           GDP H + F +DQ +FLL+RKL
Sbjct: 126 GDPAHISHFELDQRLFLLMRKL 146

The following BLAST results are available for this feature:

BLAST of Carg05790 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022929200.1	3.4e-76	100.00	DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata] >...	[more]
XP_022983196.1	9.3e-74	94.48	DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita maxima] >XP...	[more]
XP_038901286.1	1.2e-73	96.53	DNA-directed RNA polymerases II, IV and V subunit 8B-like [Benincasa hispida]	[more]
KAG6600626.1	3.5e-73	93.79	DNA-directed RNA polymerases II, IV and V subunit 8B, partial [Cucurbita argyros...	[more]
XP_022942165.1	1.0e-72	93.79	DNA-directed RNA polymerases II, IV and V subunit 8B-like [Cucurbita moschata]	[more]

BLAST of Carg05790 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M1A8	8.8e-43	57.04	DNA-directed RNA polymerases II, IV and V subunit 8B OS=Arabidopsis thaliana OX=...	[more]
O81097	1.2e-42	57.75	DNA-directed RNA polymerases II and V subunit 8A OS=Arabidopsis thaliana OX=3702...	[more]
Q19826	5.9e-31	42.76	Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhab...	[more]
A8XT81	6.6e-30	42.07	Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhab...	[more]
Q54YW8	6.6e-30	46.53	DNA-directed RNA polymerases I, II, and III subunit rpabc3 OS=Dictyostelium disc...	[more]

BLAST of Carg05790 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1ETP8	1.7e-76	100.00	DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita moschata ...	[more]
A0A6J1J454	1.7e-76	100.00	DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita maxima OX...	[more]
A0A6J1J6Q9	4.5e-74	94.48	DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita maxima OX...	[more]
A0A6J1FVR2	5.0e-73	93.79	DNA-directed RNA polymerases II, IV and V subunit 8B-like OS=Cucurbita moschata ...	[more]
A0A0A0KZC2	1.5e-72	94.48	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G031020 PE=3 SV=1	[more]

BLAST of Carg05790 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G59600.1	6.3e-44	57.04	RNA polymerase Rpb8	[more]
AT1G54250.1	8.2e-44	57.75	RNA polymerase Rpb8	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005570	RNA polymerase, Rpb8	SMART	SM00658	rpol8neu	coord: 2..144 e-value: 2.1E-58 score: 210.1
IPR005570	RNA polymerase, Rpb8	PIRSF	PIRSF000779	RPB8	coord: 1..145 e-value: 9.6E-48 score: 159.9
IPR005570	RNA polymerase, Rpb8	PFAM	PF03870	RNA_pol_Rpb8	coord: 7..144 e-value: 8.0E-50 score: 168.6
IPR005570	RNA polymerase, Rpb8	PANTHER	PTHR10917	DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3	coord: 1..145
None	No IPR available	GENE3D	2.40.50.140		coord: 6..145 e-value: 2.8E-53 score: 181.8
None	No IPR available	PANTHER	PTHR10917:SF1	DNA-DIRECTED RNA POLYMERASE I, II	coord: 1..145
IPR012340	Nucleic acid-binding, OB-fold	SUPERFAMILY	50249	Nucleic acid-binding proteins	coord: 3..144

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg05790-RA	Carg05790-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006351	transcription, DNA-templated
cellular_component	GO:0005736	RNA polymerase I complex
cellular_component	GO:0005665	RNA polymerase II, core complex
cellular_component	GO:0005666	RNA polymerase III complex
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity