Carg05779 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg05779
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Proline-rich receptor-like protein kinase PERK2
Location	Carg_Chr16: 4414702 .. 4415046 (+)
RNA-Seq Expression	Carg05779
Synteny	Carg05779

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCTTCCAAGTTCTTGATCATTGCTTTCATGGCTACTTTGGCTCTTTCAAGCATCGACATCGGTCAAGCTGCTCGCCATCTTTTACAAACTACTCCACCCCTGTTGCCGAACTTGCCAACAATTCCAACAGGGATACCGCCCTTGCCTACTCTTCCAAATTTACCACAACCCACATTGCCTAAGGTTAATCTCCCTCCATTTCCGAGTGTCCCGGTGAACATCCCGAGCGGGGCAGCATTGCCTCCGCTCCCGAGCTTTCCATCAATTCCTACCATTCCGACTTCATTCCCTTCCATCCCATTTTTATCTCCTCCTCCTGCAACTTCTTCTTCCCCCTAA

mRNA sequence

ATGGCTTCTTCCAAGTTCTTGATCATTGCTTTCATGGCTACTTTGGCTCTTTCAAGCATCGACATCGGTCAAGCTGCTCGCCATCTTTTACAAACTACTCCACCCCTGTTGCCGAACTTGCCAACAATTCCAACAGGGATACCGCCCTTGCCTACTCTTCCAAATTTACCACAACCCACATTGCCTAAGGTTAATCTCCCTCCATTTCCGAGTGTCCCGGTGAACATCCCGAGCGGGGCAGCATTGCCTCCGCTCCCGAGCTTTCCATCAATTCCTACCATTCCGACTTCATTCCCTTCCATCCCATTTTTATCTCCTCCTCCTGCAACTTCTTCTTCCCCCTAA

Coding sequence (CDS)

ATGGCTTCTTCCAAGTTCTTGATCATTGCTTTCATGGCTACTTTGGCTCTTTCAAGCATCGACATCGGTCAAGCTGCTCGCCATCTTTTACAAACTACTCCACCCCTGTTGCCGAACTTGCCAACAATTCCAACAGGGATACCGCCCTTGCCTACTCTTCCAAATTTACCACAACCCACATTGCCTAAGGTTAATCTCCCTCCATTTCCGAGTGTCCCGGTGAACATCCCGAGCGGGGCAGCATTGCCTCCGCTCCCGAGCTTTCCATCAATTCCTACCATTCCGACTTCATTCCCTTCCATCCCATTTTTATCTCCTCCTCCTGCAACTTCTTCTTCCCCCTAA

Protein sequence

MASSKFLIIAFMATLALSSIDIGQAARHLLQTTPPLLPNLPTIPTGIPPLPTLPNLPQPTLPKVNLPPFPSVPVNIPSGAALPPLPSFPSIPTIPTSFPSIPFLSPPPATSSSP

Homology

[Close]

BLAST of Carg05779 vs. NCBI nr
Match: KAG6577257.1 (hypothetical protein SDJN03_24831, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015347.1 hypothetical protein SDJN02_22982, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 207.2 bits (526), Expect = 7.4e-50
Identity = 114/114 (100.00%), Postives = 114/114 (100.00%), Query Frame = 0


Query: 1   MASSKFLIIAFMATLALSSIDIGQAARHLLQTTPPLLPNLPTIPTGIPPLPTLPNLPQPT 60
           MASSKFLIIAFMATLALSSIDIGQAARHLLQTTPPLLPNLPTIPTGIPPLPTLPNLPQPT
Sbjct: 1   MASSKFLIIAFMATLALSSIDIGQAARHLLQTTPPLLPNLPTIPTGIPPLPTLPNLPQPT 60

Query: 61  LPKVNLPPFPSVPVNIPSGAALPPLPSFPSIPTIPTSFPSIPFLSPPPATSSSP 115
           LPKVNLPPFPSVPVNIPSGAALPPLPSFPSIPTIPTSFPSIPFLSPPPATSSSP
Sbjct: 61  LPKVNLPPFPSVPVNIPSGAALPPLPSFPSIPTIPTSFPSIPFLSPPPATSSSP 114

[Close]

BLAST of Carg05779 vs. NCBI nr
Match: XP_023552884.1 (protein PELPK1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 197.2 bits (500), Expect = 7.6e-47
Identity = 108/114 (94.74%), Postives = 110/114 (96.49%), Query Frame = 0


Query: 1   MASSKFLIIAFMATLALSSIDIGQAARHLLQTTPPLLPNLPTIPTGIPPLPTLPNLPQPT 60
           M SSKFLIIAFMATLALSSID+GQAARHLLQ TPPLLPNLPT+PTGIP LPTLPNLPQPT
Sbjct: 1   MTSSKFLIIAFMATLALSSIDMGQAARHLLQITPPLLPNLPTVPTGIPSLPTLPNLPQPT 60

Query: 61  LPKVNLPPFPSVPVNIPSGAALPPLPSFPSIPTIPTSFPSIPFLSPPPATSSSP 115
           LPKVNLPPFPSVPVNIPSG ALPPLPSFPSIPTIPTSFPSIPFLSPPPATSSSP
Sbjct: 61  LPKVNLPPFPSVPVNIPSGVALPPLPSFPSIPTIPTSFPSIPFLSPPPATSSSP 114

[Close]

BLAST of Carg05779 vs. NCBI nr
Match: KAA0056641.1 (proline-rich receptor-like protein kinase PERK2 [Cucumis melo var. makuwa] >TYK07567.1 proline-rich receptor-like protein kinase PERK2 [Cucumis melo var. makuwa])

HSP 1 Score: 181.8 bits (460), Expect = 3.3e-42
Identity = 106/124 (85.48%), Postives = 109/124 (87.90%), Query Frame = 0


Query: 1   MASSKFLIIAFMATLALSSIDIGQAARHLLQTTPPLLPNLPTIPTGIPPL-----PTLPN 60
           MASSKFLIIAFMATLALSSID+GQAARHLLQT PPL+PNLP IPTGIPPL     PTLPN
Sbjct: 1   MASSKFLIIAFMATLALSSIDVGQAARHLLQTAPPLVPNLPVIPTGIPPLPALSNPTLPN 60

Query: 61  LPQPTLPKVNLPPFPSVPVNIPSGAALPP-----LPSFPSIPTIPTSFPSIPFLSPPPAT 115
           LPQPTLPKVNLPP PS PVNIPSG ALPP     LP+FPSIPTIPTSFPSIPFLSPPPAT
Sbjct: 61  LPQPTLPKVNLPPLPSSPVNIPSGLALPPLPNMALPNFPSIPTIPTSFPSIPFLSPPPAT 120

[Close]

BLAST of Carg05779 vs. NCBI nr
Match: XP_038904303.1 (protein PELPK1-like [Benincasa hispida])

HSP 1 Score: 162.9 bits (411), Expect = 1.6e-36
Identity = 99/125 (79.20%), Postives = 103/125 (82.40%), Query Frame = 0


Query: 1   MASSKFLIIAFMATLALSSIDIGQAARHLLQTTPPLLPNLPTIPTGIPPLP------TLP 60
           M SSKFLIIAFMATLALSSID+GQAARHLLQT PPL+PNLP IPTGIPPLP      TLP
Sbjct: 1   MGSSKFLIIAFMATLALSSIDLGQAARHLLQTAPPLVPNLPVIPTGIPPLPTLPNPTTLP 60

Query: 61  NLPQPTLPKVNLPPFPSVPVNIPSGAALPP-----LPSFPSIPTIPTSFPSIPFLSPPPA 115
           NLPQPTLPKVNLPP       +PSG ALPP     LP+FPSIPTIPTSFPSIPFLSPPPA
Sbjct: 61  NLPQPTLPKVNLPP-------LPSGLALPPLPTMALPNFPSIPTIPTSFPSIPFLSPPPA 118

[Close]

BLAST of Carg05779 vs. NCBI nr
Match: KAA0056642.1 (proline-rich receptor-like protein kinase PERK2 [Cucumis melo var. makuwa])

HSP 1 Score: 161.0 bits (406), Expect = 6.1e-36
Identity = 97/124 (78.23%), Postives = 103/124 (83.06%), Query Frame = 0


Query: 1   MASSKFLIIAFMATLALSSIDIGQAARHLLQTTPPLLPNLPTIPTGIPPL-----PTLPN 60
           MASSKFLIIAFMATLALSSI+ GQAARHLLQTTPPL+PNLP IPTGIPP      PTLPN
Sbjct: 1   MASSKFLIIAFMATLALSSINFGQAARHLLQTTPPLMPNLPAIPTGIPPFPALPNPTLPN 60

Query: 61  LPQPTLPKVNLPPFPSVPVNIPSGAALPP-----LPSFPSIPTIPTSFPSIPFLSPPPAT 115
           LPQPT+PKVNLPP       +P+G ALPP     LP+FPSIPTIPTSFPSIPFLSPPPAT
Sbjct: 61  LPQPTIPKVNLPP-------LPNGLALPPLPTMALPNFPSIPTIPTSFPSIPFLSPPPAT 117

[Close]

BLAST of Carg05779 vs. ExPASy TrEMBL
Match: A0A5D3C6P2 (Proline-rich receptor-like protein kinase PERK2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold544G00400 PE=4 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.6e-42
Identity = 106/124 (85.48%), Postives = 109/124 (87.90%), Query Frame = 0


Query: 1   MASSKFLIIAFMATLALSSIDIGQAARHLLQTTPPLLPNLPTIPTGIPPL-----PTLPN 60
           MASSKFLIIAFMATLALSSID+GQAARHLLQT PPL+PNLP IPTGIPPL     PTLPN
Sbjct: 1   MASSKFLIIAFMATLALSSIDVGQAARHLLQTAPPLVPNLPVIPTGIPPLPALSNPTLPN 60

Query: 61  LPQPTLPKVNLPPFPSVPVNIPSGAALPP-----LPSFPSIPTIPTSFPSIPFLSPPPAT 115
           LPQPTLPKVNLPP PS PVNIPSG ALPP     LP+FPSIPTIPTSFPSIPFLSPPPAT
Sbjct: 61  LPQPTLPKVNLPPLPSSPVNIPSGLALPPLPNMALPNFPSIPTIPTSFPSIPFLSPPPAT 120

[Close]

BLAST of Carg05779 vs. ExPASy TrEMBL
Match: A0A5A7ULD9 (Proline-rich receptor-like protein kinase PERK2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold288G001670 PE=4 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 2.9e-36
Identity = 97/124 (78.23%), Postives = 103/124 (83.06%), Query Frame = 0


Query: 1   MASSKFLIIAFMATLALSSIDIGQAARHLLQTTPPLLPNLPTIPTGIPPL-----PTLPN 60
           MASSKFLIIAFMATLALSSI+ GQAARHLLQTTPPL+PNLP IPTGIPP      PTLPN
Sbjct: 1   MASSKFLIIAFMATLALSSINFGQAARHLLQTTPPLMPNLPAIPTGIPPFPALPNPTLPN 60

Query: 61  LPQPTLPKVNLPPFPSVPVNIPSGAALPP-----LPSFPSIPTIPTSFPSIPFLSPPPAT 115
           LPQPT+PKVNLPP       +P+G ALPP     LP+FPSIPTIPTSFPSIPFLSPPPAT
Sbjct: 61  LPQPTIPKVNLPP-------LPNGLALPPLPTMALPNFPSIPTIPTSFPSIPFLSPPPAT 117

[Close]

BLAST of Carg05779 vs. ExPASy TrEMBL
Match: A0A5D3C8R8 (Proline-rich receptor-like protein kinase PERK2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold544G00410 PE=4 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 3.8e-36
Identity = 97/124 (78.23%), Postives = 103/124 (83.06%), Query Frame = 0


Query: 1   MASSKFLIIAFMATLALSSIDIGQAARHLLQTTPPLLPNLPTIPTGIPPL-----PTLPN 60
           MASSKFLIIAFMATLALSSI+ GQAARHLLQTTPPL+PNLP IPTGIPP      PTLPN
Sbjct: 1   MASSKFLIIAFMATLALSSINFGQAARHLLQTTPPLVPNLPAIPTGIPPFPALPNPTLPN 60

Query: 61  LPQPTLPKVNLPPFPSVPVNIPSGAALPP-----LPSFPSIPTIPTSFPSIPFLSPPPAT 115
           LPQPT+PKVNLPP       +P+G ALPP     LP+FPSIPTIPTSFPSIPFLSPPPAT
Sbjct: 61  LPQPTIPKVNLPP-------LPNGLALPPLPTMALPNFPSIPTIPTSFPSIPFLSPPPAT 117

[Close]

BLAST of Carg05779 vs. ExPASy TrEMBL
Match: A0A1S3CJJ1 (proline-rich receptor-like protein kinase PERK2 OS=Cucumis melo OX=3656 GN=LOC103501166 PE=4 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 3.8e-36
Identity = 97/124 (78.23%), Postives = 103/124 (83.06%), Query Frame = 0


Query: 1   MASSKFLIIAFMATLALSSIDIGQAARHLLQTTPPLLPNLPTIPTGIPPL-----PTLPN 60
           MASSKFLIIAFMATLALSSI+ GQAARHLLQTTPPL+PNLP IPTGIPP      PTLPN
Sbjct: 1   MASSKFLIIAFMATLALSSINFGQAARHLLQTTPPLVPNLPAIPTGIPPFPALPNPTLPN 60

Query: 61  LPQPTLPKVNLPPFPSVPVNIPSGAALPP-----LPSFPSIPTIPTSFPSIPFLSPPPAT 115
           LPQPT+PKVNLPP       +P+G ALPP     LP+FPSIPTIPTSFPSIPFLSPPPAT
Sbjct: 61  LPQPTIPKVNLPP-------LPNGLALPPLPTMALPNFPSIPTIPTSFPSIPFLSPPPAT 117

[Close]

BLAST of Carg05779 vs. ExPASy TrEMBL
Match: A0A0A0L1Y3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G374890 PE=4 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 4.1e-30
Identity = 88/124 (70.97%), Postives = 95/124 (76.61%), Query Frame = 0


Query: 1   MASSKFLIIAFMATLALSSIDIGQAARHLLQTTPPLLPNLPTIPTGIPPL-----PTLPN 60
           MA+S+FLIIAF ATLALSSI+ G AARHL QTTPPL+PNLP IPT IPPL     PTLP 
Sbjct: 1   MATSRFLIIAFTATLALSSINFGHAARHLSQTTPPLVPNLPAIPTLIPPLPGLPNPTLPK 60

Query: 61  LPQPTLPKVNLPPFPSVPVNIPSGAALPPLPS-----FPSIPTIPTSFPSIPFLSPPPAT 115
           LPQPTLPK+N PP       +P G ALPPLP+     FP IPTIP SFPSIPFLSPPPAT
Sbjct: 61  LPQPTLPKINFPP-------LPIGLALPPLPTMTLPKFPPIPTIPISFPSIPFLSPPPAT 117

[Close]

BLAST of Carg05779 vs. TAIR 10
Match: AT2G22510.1 (hydroxyproline-rich glycoprotein family protein )

HSP 1 Score: 49.7 bits (117), Expect = 1.8e-06
Identity = 49/120 (40.83%), Postives = 69/120 (57.50%), Query Frame = 0


Query: 7   LIIAFMATLALSSIDIGQAARHLLQTTPPLLP----NLPTIP-TGIPPLPTLPNLPQPT- 66
           L+IA   T   + +    A+RHLLQ+TP + P      P +P T +PP P   +LPQPT 
Sbjct: 8   LVIALTFTSFFTGL---SASRHLLQSTPAITPPVTTTFPPLPTTTMPPFPPSTSLPQPTA 67

Query: 67  ---LPKVNLP--PFPSVPVNIPSGAALPPLPSFP-SIP-----TIPTSFPSIPFLSPPPA 110
              LP   +P  P P+ P+NIP+   +  +P+FP SIP      +PTS P+IPF +PPP+
Sbjct: 68  FPPLPSSQIPSLPNPAQPINIPNFPQI-NIPNFPISIPNNFPFNLPTSIPTIPFFTPPPS 123

[Close]

BLAST of Carg05779 vs. TAIR 10
Match: AT4G38080.1 (hydroxyproline-rich glycoprotein family protein )

HSP 1 Score: 45.8 bits (107), Expect = 2.6e-05
Identity = 47/120 (39.17%), Postives = 63/120 (52.50%), Query Frame = 0


Query: 7   LIIAFMATLALSSIDIGQAARHLLQTTPPLLPNLPTIP----TGIPPLPTLPN------- 66
           L+IA   T   +S+    A RHLLQ+TP   P   T P    T +PP+P+LP        
Sbjct: 12  LVIALTFTSFFTSL---SAHRHLLQSTPVTQPPALTFPPLPKTTMPPVPSLPTPGQQTLP 71

Query: 67  LPQPTLPK-VNLPPFPSVPVNIPSGAALPPLPSFPSIPTIPTSFP-----SIPFLSPPPA 110
            PQPTLP+   LPP PS  +        P +P+ P I   P++FP     +IPFL+PPP+
Sbjct: 72  QPQPTLPQPTGLPPMPSTQIPSLPNQVQPTIPNIPQI-NFPSNFPFNFPFNIPFLTPPPS 127

[Close]

BLAST of Carg05779 vs. TAIR 10
Match: AT5G15780.1 (Pollen Ole e 1 allergen and extensin family protein )

HSP 1 Score: 45.1 bits (105), Expect = 4.5e-05
Identity = 41/96 (42.71%), Postives = 52/96 (54.17%), Query Frame = 0


Query: 33  TPPLLPNLPTIPT----GIPPLPTLPNLPQ-PTLPKVNL-------PPFPSVPVNIPSGA 92
           TPP LP +P +PT     +PP+PT+P LP  P LP V +       PP PS PV +P   
Sbjct: 306 TPPTLPTIPLLPTPPTPTLPPIPTIPTLPPLPVLPPVPIVNPPSLPPPPPSFPVPLPPVP 365

Query: 93  ALPPLPSFPSIPTIPTS--FPSIPFLSPPPATSSSP 115
            LP +P  P IP IP +   P IP LSP  ++   P
Sbjct: 366 GLPGIPPVPLIPGIPPAPLIPGIPPLSPSFSSHHQP 401

[Close]

BLAST of Carg05779 vs. TAIR 10
Match: AT5G09520.1 (hydroxyproline-rich glycoprotein family protein )

HSP 1 Score: 41.2 bits (95), Expect = 6.5e-04
Identity = 40/112 (35.71%), Postives = 54/112 (48.21%), Query Frame = 0


Query: 4   SKFLIIAFMATLALSSIDIGQAARHLLQTTPPLLPNLPTIP-TGIPPLPTLPNLPQPTLP 63
           S FLII     +AL S+ +   AR LL+   P +P  P +P   +P LP  P L  P LP
Sbjct: 13  SPFLIICL---IALLSVPVSVGARRLLEEPKPEIPTFPELPKPEMPKLPEFPKLELPKLP 72

Query: 64  KVNLPPFPSVPVNIPSGAALPPLPSFPSIPTIPT----SFPSIPFLSPPPAT 111
           ++  P  P +P         P LP+FP +P +P      FP +P L  P  T
Sbjct: 73  EIPKPEMPKLP-----EIQKPELPTFPELPKMPEFPKFDFPKLPELPKPEET 116

The following BLAST results are available for this feature:

BLAST of Carg05779 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6577257.1	7.4e-50	100.00	hypothetical protein SDJN03_24831, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023552884.1	7.6e-47	94.74	protein PELPK1-like [Cucurbita pepo subsp. pepo]	[more]
KAA0056641.1	3.3e-42	85.48	proline-rich receptor-like protein kinase PERK2 [Cucumis melo var. makuwa] >TYK0...	[more]
XP_038904303.1	1.6e-36	79.20	protein PELPK1-like [Benincasa hispida]	[more]
KAA0056642.1	6.1e-36	78.23	proline-rich receptor-like protein kinase PERK2 [Cucumis melo var. makuwa]	[more]

BLAST of Carg05779 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg05779 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3C6P2	1.6e-42	85.48	Proline-rich receptor-like protein kinase PERK2 OS=Cucumis melo var. makuwa OX=1...	[more]
A0A5A7ULD9	2.9e-36	78.23	Proline-rich receptor-like protein kinase PERK2 OS=Cucumis melo var. makuwa OX=1...	[more]
A0A5D3C8R8	3.8e-36	78.23	Proline-rich receptor-like protein kinase PERK2 OS=Cucumis melo var. makuwa OX=1...	[more]
A0A1S3CJJ1	3.8e-36	78.23	proline-rich receptor-like protein kinase PERK2 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A0A0L1Y3	4.1e-30	70.97	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G374890 PE=4 SV=1	[more]

BLAST of Carg05779 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G22510.1	1.8e-06	40.83	hydroxyproline-rich glycoprotein family protein	[more]
AT4G38080.1	2.6e-05	39.17	hydroxyproline-rich glycoprotein family protein	[more]
AT5G15780.1	4.5e-05	42.71	Pollen Ole e 1 allergen and extensin family protein	[more]
AT5G09520.1	6.5e-04	35.71	hydroxyproline-rich glycoprotein family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR33088:SF33	PROTEIN APP1-LIKE	coord: 1..112
IPR044659	Protein PELPK-like	PANTHER	PTHR33088	MUCIN-2	coord: 1..112

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg05779-RA	Carg05779-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
molecular_function	GO:0016301	kinase activity