Carg05748 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg05748
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein
Location	Carg_Chr16: 4197110 .. 4199118 (+)
RNA-Seq Expression	Carg05748
Synteny	Carg05748

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTGTACTTCATCAAATTCTACCATTAAAAGGGGCATTGAACAACACAAAGAACTCATCCAAGTAGAGAAGAACAAGAACAAGAACCAAACCCAGAACCAGAACCAACTCCAAATCTTCACCCATATGGATATTGAAAAAGTTTTCCACATGAATGGTGGAATTGGCAACAATAGCTATGCCAAAAACTCCCATCTTCAGGTCCTCTCTCTGATATGTATATATATATATATTGAAATGTTTTTTGTCCTACTCTCTCTTTCCTTTTCTGTTTGTTTTCTAATGGCTAAACCACATGATTGTAACAGAAGAAAGGTTCAGATATGGTAAAGCACATAACAATGGAGGCCATAGAGCAAGTTTACCTTTCCACCGGCGCTCCTGCGAGCTTCGGCATCGCCGATCTGGGCTGCTCCTCCGGTCCAAATTCGCTATCGATCGTCAAAGAAATAATCCAAGCCGTTCAATCGCTAAGCTCCAGTCATCTCCAGCCGCCGCCGGAGTTCCGCGTCTATTTGAACGACCTTCCGACCAACGATTTCAATTCCATCTTCAAAGCCCTCCCCGATTTCTGCAGAGAGCTTCAAAACGAAGGGAAAAATCAAAATCCATCGGGGTTTTTCATTGGGGCCTATCCAGGCTCATTTTACCAGAGACTTTTCCCCAATAATTGCTTGCACTTTGTCTATTCCTCTTACAGCCTCCACTGGCTTTCTAGGGTTTGCCGCCATTGCTATTTCCTTTTCCCCCATTTCCTTTTTCTTTTTTTGGATTTTCTCTAATGTTTAAAATTGTTCGTTTCGGTGATCATCCTTGTAGGTTCCTGAGGGACTTCGAAATGAATTGGGGGAACCACTGAACAGAGGCACCATTTATATATCGGAGAGGAGTCCGATTTCCATAGCGGAGGCTTACGTGAAGCAATTCCGGCGAGATTTCGGGGAGTTCCTGAGGAAGCGAGCGGAGGAGGTGGTTTCCGGCGGCCGGATGGTGCTGATCTTGCTCGGAAGAGACGGTGCCGATCATGTCGATAGAGGAAATTCCTTCTTGTGGCACCTTCTTGCTCGCGCCTTCGCCATCTTAGTCTCGCAGGTAACAAAAATTAAATAAATAAACATAAAAATGTTTGCTTTATTTTTTTAAATGGCTTTTTATTTTATTTTATTTATAAATTTCCTTAGAAAAAATATATATATATATATTAAAAAAATGTAATCAAGAATCTTCAATAGTGATGTCGTGACCTGTTCATCCAGGCATGTATATCTTAACGACATTGGATTAGAGTGGATTATCGATGAGGGTCGAGCGTTTTATAACGAAATATGTATTTTGTTTGTTATATTATAGGGAAAAGTGAAAGAGGAGGAGGTAGATAGATACGACGTGAATTTCTACGCACCGAACAAAGATGAGATAGAAGAGGAAGTAAAAAGAGAAGGGTCGTTCGGGTTGGAGAGAATAGAGAAGTTTGAGTTAGAAAAGAAGGTGAAAAACGGCGAGAGCTACGGGAAAGAAGTGGCAAAGACTGTTAGAGCCATTCAAGAATCTATGATATCTCACCATTTCGGTGATGCAATTTTGGAGTCTTTGTTTCATAATTACAGTACCATTTTGGACGAAGAATCTGCCAAACAAGATATCAAACCCATCTCTTTTGTTATCGTTCTTACAAAGTTTTAAATTATTTATTTTGAAAAAATTCTTAGTCTCACATTTTTTGTAATATTTCTACTTCATTTCTTATCGATTTAGTGTCGTTTTAATTGTCTCATCTCGTTGTTCTTTTCCTAGGCTGAATATATTACAGAGTTATGAGCTTTCTGATTATGTAAAATCCCGAACATCGAGTTTGAACACTAACAACGTTGTAAAAAACTAGTTTTTTTTTAGTTCTAAACTGCTCGGGAGCTATTTTGAATCTACTACGACCAAATCGAAAAAGATTCATTATTGAAGGGCAACTAACTTTGATATAAACTCAATTAAGAGTCAAAAAGTCGTGAATTT

mRNA sequence

TTTGTACTTCATCAAATTCTACCATTAAAAGGGGCATTGAACAACACAAAGAACTCATCCAAGTAGAGAAGAACAAGAACAAGAACCAAACCCAGAACCAGAACCAACTCCAAATCTTCACCCATATGGATATTGAAAAAGTTTTCCACATGAATGGTGGAATTGGCAACAATAGCTATGCCAAAAACTCCCATCTTCAGGTCCTCTCTCTGATATGTATATATATATATATTGAAATGTTTTTTGTCCTACTCTCTCTTTCCTTTTCTGTTTGTTTTCTAATGGCTAAACCACATGATTGTAACAGAAGAAAGGTTCAGATATGGTAAAGCACATAACAATGGAGGCCATAGAGCAAGTTTACCTTTCCACCGGCGCTCCTGCGAGCTTCGGCATCGCCGATCTGGGCTGCTCCTCCGGTCCAAATTCGCTATCGATCGTCAAAGAAATAATCCAAGCCGTTCAATCGCTAAGCTCCAGTCATCTCCAGCCGCCGCCGGAGTTCCGCGTCTATTTGAACGACCTTCCGACCAACGATTTCAATTCCATCTTCAAAGCCCTCCCCGATTTCTGCAGAGAGCTTCAAAACGAAGGGAAAAATCAAAATCCATCGGGGTTTTTCATTGGGGCCTATCCAGGCTCATTTTACCAGAGACTTTTCCCCAATAATTGCTTGCACTTTGTCTATTCCTCTTACAGCCTCCACTGGCTTTCTAGGGTTTGCCGCCATTGCTATTTCCTTTTCCCCCATTTCCTTTTTCTTTTTTTGGATTTTCTCTAATGTTTAAAATTGTTCGTTTCGGTGATCATCCTTGTAGGTTCCTGAGGGACTTCGAAATGAATTGGGGGAACCACTGAACAGAGGCACCATTTATATATCGGAGAGGAGTCCGATTTCCATAGCGGAGGCTTACGTGAAGCAATTCCGGCGAGATTTCGGGGAGTTCCTGAGGAAGCGAGCGGAGGAGGTGGTTTCCGGCGGCCGGATGGTGCTGATCTTGCTCGGAAGAGACGGTGCCGATCATGTCGATAGAGGAAATTCCTTCTTGTGGCACCTTCTTGCTCGCGCCTTCGCCATCTTAGTCTCGCAGGGAAAAGTGAAAGAGGAGGAGGTAGATAGATACGACGTGAATTTCTACGCACCGAACAAAGATGAGATAGAAGAGGAAGTAAAAAGAGAAGGGTCGTTCGGGTTGGAGAGAATAGAGAAGTTTGAGTTAGAAAAGAAGGTGAAAAACGGCGAGAGCTACGGGAAAGAAGTGGCAAAGACTGTTAGAGCCATTCAAGAATCTATGATATCTCACCATTTCGGTGATGCAATTTTGGAGTCTTTGTTTCATAATTACAGTACCATTTTGGACGAAGAATCTGCCAAACAAGATATCAAACCCATCTCTTTTGTTATCGTTCTTACAAAGTTTTAAATTATTTATTTTGAAAAAATTCTTAGTCTCACATTTTTTGTAATATTTCTACTTCATTTCTTATCGATTTAGTGTCGTTTTAATTGTCTCATCTCGTTGTTCTTTTCCTAGGCTGAATATATTACAGAGTTATGAGCTTTCTGATTATGTAAAATCCCGAACATCGAGTTTGAACACTAACAACGTTGTAAAAAACTAGTTTTTTTTTAGTTCTAAACTGCTCGGGAGCTATTTTGAATCTACTACGACCAAATCGAAAAAGATTCATTATTGAAGGGCAACTAACTTTGATATAAACTCAATTAAGAGTCAAAAAGTCGTGAATTT

Coding sequence (CDS)

ATGGTAAAGCACATAACAATGGAGGCCATAGAGCAAGTTTACCTTTCCACCGGCGCTCCTGCGAGCTTCGGCATCGCCGATCTGGGCTGCTCCTCCGGTCCAAATTCGCTATCGATCGTCAAAGAAATAATCCAAGCCGTTCAATCGCTAAGCTCCAGTCATCTCCAGCCGCCGCCGGAGTTCCGCGTCTATTTGAACGACCTTCCGACCAACGATTTCAATTCCATCTTCAAAGCCCTCCCCGATTTCTGCAGAGAGCTTCAAAACGAAGGGAAAAATCAAAATCCATCGGGGTTTTTCATTGGGGCCTATCCAGGCTCATTTTACCAGAGACTTTTCCCCAATAATTGCTTGCACTTTGTCTATTCCTCTTACAGCCTCCACTGGCTTTCTAGGGTTTGCCGCCATTGCTATTTCCTTTTCCCCCATTTCCTTTTTCTTTTTTTGGATTTTCTCTAA

Protein sequence

MVKHITMEAIEQVYLSTGAPASFGIADLGCSSGPNSLSIVKEIIQAVQSLSSSHLQPPPEFRVYLNDLPTNDFNSIFKALPDFCRELQNEGKNQNPSGFFIGAYPGSFYQRLFPNNCLHFVYSSYSLHWLSRVCRHCYFLFPHFLFLFLDFL

Homology

[Close]

BLAST of Carg05748 vs. NCBI nr
Match: KAG7015316.1 (Salicylate carboxymethyltransferase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 314.7 bits (805), Expect = 4.4e-82
Identity = 152/152 (100.00%), Postives = 152/152 (100.00%), Query Frame = 0


Query: 1   MVKHITMEAIEQVYLSTGAPASFGIADLGCSSGPNSLSIVKEIIQAVQSLSSSHLQPPPE 60
           MVKHITMEAIEQVYLSTGAPASFGIADLGCSSGPNSLSIVKEIIQAVQSLSSSHLQPPPE
Sbjct: 1   MVKHITMEAIEQVYLSTGAPASFGIADLGCSSGPNSLSIVKEIIQAVQSLSSSHLQPPPE 60

Query: 61  FRVYLNDLPTNDFNSIFKALPDFCRELQNEGKNQNPSGFFIGAYPGSFYQRLFPNNCLHF 120
           FRVYLNDLPTNDFNSIFKALPDFCRELQNEGKNQNPSGFFIGAYPGSFYQRLFPNNCLHF
Sbjct: 61  FRVYLNDLPTNDFNSIFKALPDFCRELQNEGKNQNPSGFFIGAYPGSFYQRLFPNNCLHF 120

Query: 121 VYSSYSLHWLSRVCRHCYFLFPHFLFLFLDFL 153
           VYSSYSLHWLSRVCRHCYFLFPHFLFLFLDFL
Sbjct: 121 VYSSYSLHWLSRVCRHCYFLFPHFLFLFLDFL 152

[Close]

BLAST of Carg05748 vs. NCBI nr
Match: XP_022929492.1 (salicylate carboxymethyltransferase-like [Cucurbita moschata])

HSP 1 Score: 276.6 bits (706), Expect = 1.3e-70
Identity = 133/133 (100.00%), Postives = 133/133 (100.00%), Query Frame = 0


Query: 1   MVKHITMEAIEQVYLSTGAPASFGIADLGCSSGPNSLSIVKEIIQAVQSLSSSHLQPPPE 60
           MVKHITMEAIEQVYLSTGAPASFGIADLGCSSGPNSLSIVKEIIQAVQSLSSSHLQPPPE
Sbjct: 31  MVKHITMEAIEQVYLSTGAPASFGIADLGCSSGPNSLSIVKEIIQAVQSLSSSHLQPPPE 90

Query: 61  FRVYLNDLPTNDFNSIFKALPDFCRELQNEGKNQNPSGFFIGAYPGSFYQRLFPNNCLHF 120
           FRVYLNDLPTNDFNSIFKALPDFCRELQNEGKNQNPSGFFIGAYPGSFYQRLFPNNCLHF
Sbjct: 91  FRVYLNDLPTNDFNSIFKALPDFCRELQNEGKNQNPSGFFIGAYPGSFYQRLFPNNCLHF 150

Query: 121 VYSSYSLHWLSRV 134
           VYSSYSLHWLSRV
Sbjct: 151 VYSSYSLHWLSRV 163

[Close]

BLAST of Carg05748 vs. NCBI nr
Match: KAG6577224.1 (Salicylate carboxymethyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 276.6 bits (706), Expect = 1.3e-70
Identity = 133/133 (100.00%), Postives = 133/133 (100.00%), Query Frame = 0


Query: 1   MVKHITMEAIEQVYLSTGAPASFGIADLGCSSGPNSLSIVKEIIQAVQSLSSSHLQPPPE 60
           MVKHITMEAIEQVYLSTGAPASFGIADLGCSSGPNSLSIVKEIIQAVQSLSSSHLQPPPE
Sbjct: 31  MVKHITMEAIEQVYLSTGAPASFGIADLGCSSGPNSLSIVKEIIQAVQSLSSSHLQPPPE 90

Query: 61  FRVYLNDLPTNDFNSIFKALPDFCRELQNEGKNQNPSGFFIGAYPGSFYQRLFPNNCLHF 120
           FRVYLNDLPTNDFNSIFKALPDFCRELQNEGKNQNPSGFFIGAYPGSFYQRLFPNNCLHF
Sbjct: 91  FRVYLNDLPTNDFNSIFKALPDFCRELQNEGKNQNPSGFFIGAYPGSFYQRLFPNNCLHF 150

Query: 121 VYSSYSLHWLSRV 134
           VYSSYSLHWLSRV
Sbjct: 151 VYSSYSLHWLSRV 163

[Close]

BLAST of Carg05748 vs. NCBI nr
Match: XP_023553174.1 (salicylate carboxymethyltransferase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 276.2 bits (705), Expect = 1.7e-70
Identity = 132/133 (99.25%), Postives = 133/133 (100.00%), Query Frame = 0


Query: 1   MVKHITMEAIEQVYLSTGAPASFGIADLGCSSGPNSLSIVKEIIQAVQSLSSSHLQPPPE 60
           MVKHITMEAIEQVYLSTGAPASFGIADLGCSSGPNSLSIVKEIIQA+QSLSSSHLQPPPE
Sbjct: 31  MVKHITMEAIEQVYLSTGAPASFGIADLGCSSGPNSLSIVKEIIQAIQSLSSSHLQPPPE 90

Query: 61  FRVYLNDLPTNDFNSIFKALPDFCRELQNEGKNQNPSGFFIGAYPGSFYQRLFPNNCLHF 120
           FRVYLNDLPTNDFNSIFKALPDFCRELQNEGKNQNPSGFFIGAYPGSFYQRLFPNNCLHF
Sbjct: 91  FRVYLNDLPTNDFNSIFKALPDFCRELQNEGKNQNPSGFFIGAYPGSFYQRLFPNNCLHF 150

Query: 121 VYSSYSLHWLSRV 134
           VYSSYSLHWLSRV
Sbjct: 151 VYSSYSLHWLSRV 163

[Close]

BLAST of Carg05748 vs. NCBI nr
Match: XP_022984428.1 (salicylate carboxymethyltransferase [Cucurbita maxima])

HSP 1 Score: 275.0 bits (702), Expect = 3.8e-70
Identity = 132/133 (99.25%), Postives = 132/133 (99.25%), Query Frame = 0


Query: 1   MVKHITMEAIEQVYLSTGAPASFGIADLGCSSGPNSLSIVKEIIQAVQSLSSSHLQPPPE 60
           MVKHITMEAIEQVYLSTG PASFGIADLGCSSGPNSLSIVKEIIQAVQSLSSSHLQPPPE
Sbjct: 31  MVKHITMEAIEQVYLSTGTPASFGIADLGCSSGPNSLSIVKEIIQAVQSLSSSHLQPPPE 90

Query: 61  FRVYLNDLPTNDFNSIFKALPDFCRELQNEGKNQNPSGFFIGAYPGSFYQRLFPNNCLHF 120
           FRVYLNDLPTNDFNSIFKALPDFCRELQNEGKNQNPSGFFIGAYPGSFYQRLFPNNCLHF
Sbjct: 91  FRVYLNDLPTNDFNSIFKALPDFCRELQNEGKNQNPSGFFIGAYPGSFYQRLFPNNCLHF 150

Query: 121 VYSSYSLHWLSRV 134
           VYSSYSLHWLSRV
Sbjct: 151 VYSSYSLHWLSRV 163

[Close]

BLAST of Carg05748 vs. ExPASy Swiss-Prot
Match: A0A061DM17 (Probable methyltransferase TCM_000336 OS=Theobroma cacao OX=3641 GN=TCM_000336 PE=3 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 1.2e-47
Identity = 90/133 (67.67%), Postives = 107/133 (80.45%), Query Frame = 0


Query: 1   MVKHITMEAIEQVYLSTGAPASFGIADLGCSSGPNSLSIVKEIIQAVQSLSSSHLQPPPE 60
           MVKHITME IE++Y S  AP S GIADLGCSSGPNSLS++K+I++ V+  S     P PE
Sbjct: 31  MVKHITMETIEELYYSI-APKSLGIADLGCSSGPNSLSLIKDIVEVVEETSHKLFHPLPE 90

Query: 61  FRVYLNDLPTNDFNSIFKALPDFCRELQNEGKNQNPSGFFIGAYPGSFYQRLFPNNCLHF 120
           FRVYLNDLPTNDFNS+FK+LPDF R+L+ + +N+     FI  YPGSFY RLFPNNCLHF
Sbjct: 91  FRVYLNDLPTNDFNSVFKSLPDFYRDLKKD-RNEGGPAIFIAGYPGSFYGRLFPNNCLHF 150

Query: 121 VYSSYSLHWLSRV 134
           +YSSYSLHWLS+V
Sbjct: 151 IYSSYSLHWLSKV 161

[Close]

BLAST of Carg05748 vs. ExPASy Swiss-Prot
Match: Q9SBK6 (Jasmonate O-methyltransferase OS=Brassica rapa subsp. pekinensis OX=51351 GN=JMT PE=1 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 3.4e-29
Identity = 65/139 (46.76%), Postives = 87/139 (62.59%), Query Frame = 0


Query: 3   KHITMEAIEQVYLSTGAPASFGIADLGCSSGPNSLSIVKEIIQAVQSLSSSHLQPPPEFR 62
           + +  EA++++ +      SFGIADLGCSSGPNSL  +  I++ +Q+L     +P PE  
Sbjct: 33  RRVMDEALKKLMIRNSEILSFGIADLGCSSGPNSLLSISNIVETIQNLCHDLDRPVPELS 92

Query: 63  VYLNDLPTNDFNSIFKALPDFCRELQNEGKNQNPSGF--------FIGAYPGSFYQRLFP 122
           + LNDLP+NDFN IF +LP+F   ++    N    GF        F+ A PGSFY RLFP
Sbjct: 93  LSLNDLPSNDFNYIFASLPEFYDRVKKRDNNYESLGFEHGSGGPCFVSAVPGSFYGRLFP 152

Query: 123 NNCLHFVYSSYSLHWLSRV 134
              LHFV+SS SLHWLS+V
Sbjct: 153 RRSLHFVHSSSSLHWLSQV 171

[Close]

BLAST of Carg05748 vs. ExPASy Swiss-Prot
Match: A0A061FBW2 (Probable jasmonic acid carboxyl methyltransferase 2 OS=Theobroma cacao OX=3641 GN=TCM_034091 PE=3 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 2.9e-28
Identity = 69/131 (52.67%), Postives = 86/131 (65.65%), Query Frame = 0


Query: 3   KHITMEAIEQVYLSTGAPASFGIADLGCSSGPNSLSIVKEIIQAVQSLSSSHLQPPPEFR 62
           K I  EA+ ++  +     S GIADLGCSSGPN+LS++ EI+  VQ+ S    +P PEFR
Sbjct: 33  KPIIEEAVHEISCN-NLLESMGIADLGCSSGPNTLSVISEIMDMVQTTSRRLGRPVPEFR 92

Query: 63  VYLNDLPTNDFNSIFKALPDFCRELQNEGKNQNPSGFFIGAYPGSFYQRLFPNNCLHFVY 122
           VYLNDL +NDFN IF +LP F   L+ E K       +I    GSFY RLFP+  LHFV+
Sbjct: 93  VYLNDLYSNDFNYIFMSLPAFYHRLKEE-KGIGCGSCYISGVAGSFYGRLFPSKSLHFVH 152

Query: 123 SSYSLHWLSRV 134
           SS SLHWLS+V
Sbjct: 153 SSSSLHWLSQV 161

[Close]

BLAST of Carg05748 vs. ExPASy Swiss-Prot
Match: A0A061FDP1 (Probable jasmonic acid carboxyl methyltransferase 1 OS=Theobroma cacao OX=3641 GN=TCM_034089 PE=3 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 4.9e-28
Identity = 68/131 (51.91%), Postives = 85/131 (64.89%), Query Frame = 0


Query: 3   KHITMEAIEQVYLSTGAPASFGIADLGCSSGPNSLSIVKEIIQAVQSLSSSHLQPPPEFR 62
           K I  EA+ ++  +     S GIADLGCSSGPN+L ++ EI+  VQ+ S     P PEFR
Sbjct: 33  KPIIEEAVHEISCN-NVLESMGIADLGCSSGPNTLCVISEIMDMVQATSHRLGHPVPEFR 92

Query: 63  VYLNDLPTNDFNSIFKALPDFCRELQNEGKNQNPSGFFIGAYPGSFYQRLFPNNCLHFVY 122
           +YLNDL +NDFNSIF +LP F   L+ E K       FI    GSFY RLFP+  LH+V+
Sbjct: 93  LYLNDLYSNDFNSIFMSLPAFYHRLKEE-KGIGCGSCFISGVAGSFYGRLFPSKSLHYVH 152

Query: 123 SSYSLHWLSRV 134
           SS SLHWLS+V
Sbjct: 153 SSSSLHWLSQV 161

[Close]

BLAST of Carg05748 vs. ExPASy Swiss-Prot
Match: Q9AR07 (Jasmonate O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=JMT PE=1 SV=3)

HSP 1 Score: 125.6 bits (314), Expect = 4.9e-28
Identity = 68/142 (47.89%), Postives = 89/142 (62.68%), Query Frame = 0


Query: 3   KHITMEAIEQVYLSTGAPASFGIADLGCSSGPNSLSIVKEIIQAVQSLSSSHLQPPPEFR 62
           + +  EA++++ +S    +S GIADLGCSSGPNSL  +  I+  + +L     +P PE R
Sbjct: 33  RRVMDEALKKLMMSNSEISSIGIADLGCSSGPNSLLSISNIVDTIHNLCPDLDRPVPELR 92

Query: 63  VYLNDLPTNDFNSIFKALPDFCRELQNEGKNQNPSGF--------FIGAYPGSFYQRLFP 122
           V LNDLP+NDFN I  +LP+F   + N   N+   GF        F+ A PGSFY RLFP
Sbjct: 93  VSLNDLPSNDFNYICASLPEFYDRVNN---NKEGLGFGRGGGESCFVSAVPGSFYGRLFP 152

Query: 123 NNCLHFVYSSYSLHWLSRV-CR 136
              LHFV+SS SLHWLS+V CR
Sbjct: 153 RRSLHFVHSSSSLHWLSQVPCR 171

[Close]

BLAST of Carg05748 vs. ExPASy TrEMBL
Match: A0A6J1EMX7 (salicylate carboxymethyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111436043 PE=4 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 6.4e-71
Identity = 133/133 (100.00%), Postives = 133/133 (100.00%), Query Frame = 0


Query: 1   MVKHITMEAIEQVYLSTGAPASFGIADLGCSSGPNSLSIVKEIIQAVQSLSSSHLQPPPE 60
           MVKHITMEAIEQVYLSTGAPASFGIADLGCSSGPNSLSIVKEIIQAVQSLSSSHLQPPPE
Sbjct: 31  MVKHITMEAIEQVYLSTGAPASFGIADLGCSSGPNSLSIVKEIIQAVQSLSSSHLQPPPE 90

Query: 61  FRVYLNDLPTNDFNSIFKALPDFCRELQNEGKNQNPSGFFIGAYPGSFYQRLFPNNCLHF 120
           FRVYLNDLPTNDFNSIFKALPDFCRELQNEGKNQNPSGFFIGAYPGSFYQRLFPNNCLHF
Sbjct: 91  FRVYLNDLPTNDFNSIFKALPDFCRELQNEGKNQNPSGFFIGAYPGSFYQRLFPNNCLHF 150

Query: 121 VYSSYSLHWLSRV 134
           VYSSYSLHWLSRV
Sbjct: 151 VYSSYSLHWLSRV 163

[Close]

BLAST of Carg05748 vs. ExPASy TrEMBL
Match: A0A6J1JAG4 (salicylate carboxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111482729 PE=4 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 1.9e-70
Identity = 132/133 (99.25%), Postives = 132/133 (99.25%), Query Frame = 0


Query: 1   MVKHITMEAIEQVYLSTGAPASFGIADLGCSSGPNSLSIVKEIIQAVQSLSSSHLQPPPE 60
           MVKHITMEAIEQVYLSTG PASFGIADLGCSSGPNSLSIVKEIIQAVQSLSSSHLQPPPE
Sbjct: 31  MVKHITMEAIEQVYLSTGTPASFGIADLGCSSGPNSLSIVKEIIQAVQSLSSSHLQPPPE 90

Query: 61  FRVYLNDLPTNDFNSIFKALPDFCRELQNEGKNQNPSGFFIGAYPGSFYQRLFPNNCLHF 120
           FRVYLNDLPTNDFNSIFKALPDFCRELQNEGKNQNPSGFFIGAYPGSFYQRLFPNNCLHF
Sbjct: 91  FRVYLNDLPTNDFNSIFKALPDFCRELQNEGKNQNPSGFFIGAYPGSFYQRLFPNNCLHF 150

Query: 121 VYSSYSLHWLSRV 134
           VYSSYSLHWLSRV
Sbjct: 151 VYSSYSLHWLSRV 163

[Close]

BLAST of Carg05748 vs. ExPASy TrEMBL
Match: A0A5A7TNL9 (Salicylate carboxymethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G005050 PE=4 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 1.7e-63
Identity = 120/133 (90.23%), Postives = 128/133 (96.24%), Query Frame = 0


Query: 1   MVKHITMEAIEQVYLSTGAPASFGIADLGCSSGPNSLSIVKEIIQAVQSLSSSHLQPPPE 60
           MVKHITMEAIE+VYLSTGAP SFGIADLGCSSGPN+LSI+KEIIQAVQSLSS HL+  PE
Sbjct: 23  MVKHITMEAIEKVYLSTGAPTSFGIADLGCSSGPNTLSIIKEIIQAVQSLSSDHLRQSPE 82

Query: 61  FRVYLNDLPTNDFNSIFKALPDFCRELQNEGKNQNPSGFFIGAYPGSFYQRLFPNNCLHF 120
           FRVYLNDLPTNDFNSIFKALPDFCRELQNEG+NQNPSGFFIGAYPGSFY+RLFP+N LHF
Sbjct: 83  FRVYLNDLPTNDFNSIFKALPDFCRELQNEGENQNPSGFFIGAYPGSFYKRLFPSNSLHF 142

Query: 121 VYSSYSLHWLSRV 134
           V+SSYSLHWLSRV
Sbjct: 143 VHSSYSLHWLSRV 155

[Close]

BLAST of Carg05748 vs. ExPASy TrEMBL
Match: A0A0A0KUR7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G046610 PE=4 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 1.7e-63
Identity = 120/133 (90.23%), Postives = 126/133 (94.74%), Query Frame = 0


Query: 1   MVKHITMEAIEQVYLSTGAPASFGIADLGCSSGPNSLSIVKEIIQAVQSLSSSHLQPPPE 60
           MVKH+TMEAIE+VYLSTGAP SFGIADLGCSSGPN+LSIVKEIIQA QSLSS HL+   E
Sbjct: 31  MVKHVTMEAIEKVYLSTGAPTSFGIADLGCSSGPNTLSIVKEIIQAFQSLSSDHLRQSSE 90

Query: 61  FRVYLNDLPTNDFNSIFKALPDFCRELQNEGKNQNPSGFFIGAYPGSFYQRLFPNNCLHF 120
           FRVYLNDLPTNDFNSIFKALPDFCRELQNEG NQNPSGFFIGAYPGSFYQRLFP+NCLHF
Sbjct: 91  FRVYLNDLPTNDFNSIFKALPDFCRELQNEGVNQNPSGFFIGAYPGSFYQRLFPSNCLHF 150

Query: 121 VYSSYSLHWLSRV 134
           VYS+YSLHWLSRV
Sbjct: 151 VYSNYSLHWLSRV 163

[Close]

BLAST of Carg05748 vs. ExPASy TrEMBL
Match: A0A1S3BSG5 (salicylate carboxymethyltransferase OS=Cucumis melo OX=3656 GN=LOC103493203 PE=4 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 1.7e-63
Identity = 120/133 (90.23%), Postives = 128/133 (96.24%), Query Frame = 0


Query: 1   MVKHITMEAIEQVYLSTGAPASFGIADLGCSSGPNSLSIVKEIIQAVQSLSSSHLQPPPE 60
           MVKHITMEAIE+VYLSTGAP SFGIADLGCSSGPN+LSI+KEIIQAVQSLSS HL+  PE
Sbjct: 31  MVKHITMEAIEKVYLSTGAPTSFGIADLGCSSGPNTLSIIKEIIQAVQSLSSDHLRQSPE 90

Query: 61  FRVYLNDLPTNDFNSIFKALPDFCRELQNEGKNQNPSGFFIGAYPGSFYQRLFPNNCLHF 120
           FRVYLNDLPTNDFNSIFKALPDFCRELQNEG+NQNPSGFFIGAYPGSFY+RLFP+N LHF
Sbjct: 91  FRVYLNDLPTNDFNSIFKALPDFCRELQNEGENQNPSGFFIGAYPGSFYKRLFPSNSLHF 150

Query: 121 VYSSYSLHWLSRV 134
           V+SSYSLHWLSRV
Sbjct: 151 VHSSYSLHWLSRV 163

[Close]

BLAST of Carg05748 vs. TAIR 10
Match: AT4G36470.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 157.1 bits (396), Expect = 1.1e-38
Identity = 77/133 (57.89%), Postives = 96/133 (72.18%), Query Frame = 0


Query: 3   KHITMEAIEQVYLSTGAPASFGIADLGCSSGPNSLSIVKEIIQAVQSLSSSH--LQPPPE 62
           KHIT+E ++Q+Y  T  P S GIADLGCSSGPN+LS + + I+ VQ        +QP PE
Sbjct: 36  KHITLETLQQLYKET-RPKSLGIADLGCSSGPNTLSTITDFIKTVQVAHHREIPIQPLPE 95

Query: 63  FRVYLNDLPTNDFNSIFKALPDFCRELQNEGKNQNPSGFFIGAYPGSFYQRLFPNNCLHF 122
           F ++LNDLP NDFN IFK+LPDF  EL+ +  N +    FI AYPGSFY RLFP N +HF
Sbjct: 96  FSIFLNDLPGNDFNFIFKSLPDFHIELKRDNNNGDCPSVFIAAYPGSFYGRLFPENTIHF 155

Query: 123 VYSSYSLHWLSRV 134
           VY+S+SLHWLS+V
Sbjct: 156 VYASHSLHWLSKV 167

[Close]

BLAST of Carg05748 vs. TAIR 10
Match: AT1G19640.1 (jasmonic acid carboxyl methyltransferase )

HSP 1 Score: 125.6 bits (314), Expect = 3.5e-29
Identity = 68/142 (47.89%), Postives = 89/142 (62.68%), Query Frame = 0


Query: 3   KHITMEAIEQVYLSTGAPASFGIADLGCSSGPNSLSIVKEIIQAVQSLSSSHLQPPPEFR 62
           + +  EA++++ +S    +S GIADLGCSSGPNSL  +  I+  + +L     +P PE R
Sbjct: 33  RRVMDEALKKLMMSNSEISSIGIADLGCSSGPNSLLSISNIVDTIHNLCPDLDRPVPELR 92

Query: 63  VYLNDLPTNDFNSIFKALPDFCRELQNEGKNQNPSGF--------FIGAYPGSFYQRLFP 122
           V LNDLP+NDFN I  +LP+F   + N   N+   GF        F+ A PGSFY RLFP
Sbjct: 93  VSLNDLPSNDFNYICASLPEFYDRVNN---NKEGLGFGRGGGESCFVSAVPGSFYGRLFP 152

Query: 123 NNCLHFVYSSYSLHWLSRV-CR 136
              LHFV+SS SLHWLS+V CR
Sbjct: 153 RRSLHFVHSSSSLHWLSQVPCR 171

[Close]

BLAST of Carg05748 vs. TAIR 10
Match: AT2G14060.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 113.2 bits (282), Expect = 1.8e-25
Identity = 53/132 (40.15%), Postives = 78/132 (59.09%), Query Frame = 0


Query: 2   VKHITMEAIEQVYLSTGAPASFGIADLGCSSGPNSLSIVKEIIQAVQSLSSSHLQPPPEF 61
           ++ + +E + ++ L  G P    +ADLGCS+G N++  +  I   +        + PPE 
Sbjct: 24  IQPLVIENVREMLLKNGFPGCIKVADLGCSTGQNTVLAMSAIAYTIMESYQQMSKNPPEI 83

Query: 62  RVYLNDLPTNDFNSIFKALPDFCRELQNEGKNQNPSGFFIGAYPGSFYQRLFPNNCLHFV 121
             YLNDLP NDFN+ FK    F  +L+ E K +    +F+   PGSFY RLFP   LHFV
Sbjct: 84  DCYLNDLPENDFNTTFKLFHSFQEKLKPEVKGK----WFVSGVPGSFYSRLFPRKSLHFV 143

Query: 122 YSSYSLHWLSRV 134
           +S++S+HWLSR+
Sbjct: 144 HSAFSIHWLSRI 151

[Close]

BLAST of Carg05748 vs. TAIR 10
Match: AT5G38020.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 112.1 bits (279), Expect = 4.0e-25
Identity = 56/131 (42.75%), Postives = 80/131 (61.07%), Query Frame = 0


Query: 3   KHITMEAIEQVYLSTGAPASFGIADLGCSSGPNSLSIVKEIIQAVQSLSSSHLQPPPEFR 62
           K + +E ++++ + T  P    +ADLGCSSG N+L ++ EI+  + +      +  PE  
Sbjct: 30  KPVVVETVKEMIVKTDFPGCIKVADLGCSSGENTLLVMSEIVNTIITSYQQKGKNLPEIN 89

Query: 63  VYLNDLPTNDFNSIFKALPDFCRELQNEGKNQNPSGFFIGAYPGSFYQRLFPNNCLHFVY 122
             LNDLP NDFN+ FK +P F + L+ + K +     FI   PGSFY RLFP+  LHFV+
Sbjct: 90  CCLNDLPDNDFNTTFKLVPAFHKLLKMDVKGK----CFISGVPGSFYSRLFPSKSLHFVH 149

Query: 123 SSYSLHWLSRV 134
           SS  LHWLS+V
Sbjct: 150 SSLCLHWLSKV 156

[Close]

BLAST of Carg05748 vs. TAIR 10
Match: AT3G11480.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 108.2 bits (269), Expect = 5.8e-24
Identity = 57/133 (42.86%), Postives = 76/133 (57.14%), Query Frame = 0


Query: 1   MVKHITMEAIEQVYLSTGAPASFGIADLGCSSGPNSLSIVKEIIQAVQSLSSSHLQPPPE 60
           M K + +   E++ ++   P    +A+LGCSSG NS   + EII  +  L     +  PE
Sbjct: 53  MAKPVLVRNTEEMMMNLDFPTYIKVAELGCSSGQNSFLAIFEIINTINVLCQHVNKNSPE 112

Query: 61  FRVYLNDLPTNDFNSIFKALPDFCRELQNEGKNQNPSGFFIGAYPGSFYQRLFPNNCLHF 120
               LNDLP NDFN+ FK +P F +EL       N S  F+   PGSFY RLF  N LH 
Sbjct: 113 IDCCLNDLPENDFNTTFKFVPFFNKELM----ITNKSSCFVYGAPGSFYSRLFSRNSLHL 172

Query: 121 VYSSYSLHWLSRV 134
           ++SSY+LHWLS+V
Sbjct: 173 IHSSYALHWLSKV 181

The following BLAST results are available for this feature:

BLAST of Carg05748 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7015316.1	4.4e-82	100.00	Salicylate carboxymethyltransferase [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022929492.1	1.3e-70	100.00	salicylate carboxymethyltransferase-like [Cucurbita moschata]	[more]
KAG6577224.1	1.3e-70	100.00	Salicylate carboxymethyltransferase, partial [Cucurbita argyrosperma subsp. soro...	[more]
XP_023553174.1	1.7e-70	99.25	salicylate carboxymethyltransferase [Cucurbita pepo subsp. pepo]	[more]
XP_022984428.1	3.8e-70	99.25	salicylate carboxymethyltransferase [Cucurbita maxima]	[more]

BLAST of Carg05748 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A061DM17	1.2e-47	67.67	Probable methyltransferase TCM_000336 OS=Theobroma cacao OX=3641 GN=TCM_000336 P...	[more]
Q9SBK6	3.4e-29	46.76	Jasmonate O-methyltransferase OS=Brassica rapa subsp. pekinensis OX=51351 GN=JMT...	[more]
A0A061FBW2	2.9e-28	52.67	Probable jasmonic acid carboxyl methyltransferase 2 OS=Theobroma cacao OX=3641 G...	[more]
A0A061FDP1	4.9e-28	51.91	Probable jasmonic acid carboxyl methyltransferase 1 OS=Theobroma cacao OX=3641 G...	[more]
Q9AR07	4.9e-28	47.89	Jasmonate O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=JMT PE=1 SV=3	[more]

BLAST of Carg05748 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EMX7	6.4e-71	100.00	salicylate carboxymethyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1JAG4	1.9e-70	99.25	salicylate carboxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111482729 ...	[more]
A0A5A7TNL9	1.7e-63	90.23	Salicylate carboxymethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5...	[more]
A0A0A0KUR7	1.7e-63	90.23	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G046610 PE=4 SV=1	[more]
A0A1S3BSG5	1.7e-63	90.23	salicylate carboxymethyltransferase OS=Cucumis melo OX=3656 GN=LOC103493203 PE=4...	[more]

BLAST of Carg05748 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G36470.1	1.1e-38	57.89	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT1G19640.1	3.5e-29	47.89	jasmonic acid carboxyl methyltransferase	[more]
AT2G14060.1	1.8e-25	40.15	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT5G38020.1	4.0e-25	42.75	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT3G11480.1	5.8e-24	42.86	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 1..139 e-value: 3.5E-52 score: 178.8
None	No IPR available	PANTHER	PTHR31009:SF6	OS01G0701700 PROTEIN	coord: 1..134
IPR005299	SAM dependent carboxyl methyltransferase	PFAM	PF03492	Methyltransf_7	coord: 8..136 e-value: 9.9E-51 score: 172.9
IPR005299	SAM dependent carboxyl methyltransferase	PANTHER	PTHR31009	S-ADENOSYL-L-METHIONINE:CARBOXYL METHYLTRANSFERASE FAMILY PROTEIN	coord: 1..134
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 2..133

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg05748-RA	Carg05748-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0008757	S-adenosylmethionine-dependent methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity