Carg05716 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg05716 vs. NCBI nr
Match: KAG7015284.1 (putative 26S proteasome non-ATPase regulatory subunit 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 939.9 bits (2428), Expect = 9.0e-270
Identity = 493/493 (100.00%), Postives = 493/493 (100.00%), Query Frame = 0

Query: 1   MMQDAEMKELPASVSSSTSPSTLQHLKEIVSLIEAGAYAREVRRIFRAIRLTMALRRRLK 60
           MMQDAEMKELPASVSSSTSPSTLQHLKEIVSLIEAGAYAREVRRIFRAIRLTMALRRRLK
Sbjct: 1   MMQDAEMKELPASVSSSTSPSTLQHLKEIVSLIEAGAYAREVRRIFRAIRLTMALRRRLK 60

Query: 61  ASVIFSFLNFALPPGSDVHTKLLSFIPKEDDCEMEIDSAAPAAVKLPLPEIEIFCYLIVL 120
           ASVIFSFLNFALPPGSDVHTKLLSFIPKEDDCEMEIDSAAPAAVKLPLPEIEIFCYLIVL
Sbjct: 61  ASVIFSFLNFALPPGSDVHTKLLSFIPKEDDCEMEIDSAAPAAVKLPLPEIEIFCYLIVL 120

Query: 121 IFLIDQKKYDEAVACASASIARLKNLNRRTVDVLASRLYSYYSLSYQLTGNLAEIRGNLL 180
           IFLIDQKKYDEAVACASASIARLKNLNRRTVDVLASRLYSYYSLSYQLTGNLAEIRGNLL
Sbjct: 121 IFLIDQKKYDEAVACASASIARLKNLNRRTVDVLASRLYSYYSLSYQLTGNLAEIRGNLL 180

Query: 181 ALHRIATLRHDELGQETLLNLLLFNYLHYNLYDQAEKLRSKAPRFEAHSNQQASLSALFC 240
           ALHRIATLRHDELGQETLLNLLLFNYLHYNLYDQAEKLRSKAPRFEAHSNQQASLSALFC
Sbjct: 181 ALHRIATLRHDELGQETLLNLLLFNYLHYNLYDQAEKLRSKAPRFEAHSNQQASLSALFC 240

Query: 241 RYLFYLGKIRTIQLEYTDAKESLLQAARKAPTAARGFRVQCNKWAIIVRLLLGEIPERTV 300
           RYLFYLGKIRTIQLEYTDAKESLLQAARKAPTAARGFRVQCNKWAIIVRLLLGEIPERTV
Sbjct: 241 RYLFYLGKIRTIQLEYTDAKESLLQAARKAPTAARGFRVQCNKWAIIVRLLLGEIPERTV 300

Query: 301 FTQKGMEIALRPYFELTNAVRIGDLERFKTVAEKFSCTFSSDRTNNLIVRLRHNVIRTGI 360
           FTQKGMEIALRPYFELTNAVRIGDLERFKTVAEKFSCTFSSDRTNNLIVRLRHNVIRTGI
Sbjct: 301 FTQKGMEIALRPYFELTNAVRIGDLERFKTVAEKFSCTFSSDRTNNLIVRLRHNVIRTGI 360

Query: 361 HNISLSYSRISLADVAKKLRLNSENPIADAENIVTKAIRDGAIDATVDHANGWMVSKETG 420
           HNISLSYSRISLADVAKKLRLNSENPIADAENIVTKAIRDGAIDATVDHANGWMVSKETG
Sbjct: 361 HNISLSYSRISLADVAKKLRLNSENPIADAENIVTKAIRDGAIDATVDHANGWMVSKETG 420

Query: 421 DIYSTNEPQIALNSRIAFCLNMHNDAVRALRFPSNSQKEKESAEKRRERRQQEKELAKNI 480
           DIYSTNEPQIALNSRIAFCLNMHNDAVRALRFPSNSQKEKESAEKRRERRQQEKELAKNI
Sbjct: 421 DIYSTNEPQIALNSRIAFCLNMHNDAVRALRFPSNSQKEKESAEKRRERRQQEKELAKNI 480

Query: 481 AEGDDDDFLTDPM 494
           AEGDDDDFLTDPM
Sbjct: 481 AEGDDDDFLTDPM 493

BLAST of Carg05716 vs. NCBI nr
Match: XP_022928271.1 (probable 26S proteasome non-ATPase regulatory subunit 3 [Cucurbita moschata] >XP_022928273.1 probable 26S proteasome non-ATPase regulatory subunit 3 [Cucurbita moschata])

HSP 1 Score: 917.9 bits (2371), Expect = 3.7e-263
Identity = 484/493 (98.17%), Postives = 484/493 (98.17%), Query Frame = 0

Query: 1   MMQDAEMKELPASVSSSTSPSTLQHLKEIVSLIEAGAYAREVRRIFRAIRLTMALRRRLK 60
           MM DAEMKELPASVSSSTSPSTLQHLKEIVSLIEAGAYAREVRRIFRAIRLTMALRRRLK
Sbjct: 1   MMPDAEMKELPASVSSSTSPSTLQHLKEIVSLIEAGAYAREVRRIFRAIRLTMALRRRLK 60

Query: 61  ASVIFSFLNFALPPGSDVHTKLLSFIPKEDDCEMEIDSAAPAAVKLPLPEIEIFCYLIVL 120
           ASVIFSFLNFALPPGSDVHTKL SFIPKEDDCEMEIDSAAPAAVKLPLPEIEIFCYLIVL
Sbjct: 61  ASVIFSFLNFALPPGSDVHTKLSSFIPKEDDCEMEIDSAAPAAVKLPLPEIEIFCYLIVL 120

Query: 121 IFLIDQKKYDEAVACASASIARLKNLNRRTVDVLASRLYSYYSLSYQLTGNLAEIRGNLL 180
           IFLIDQKKYDEAVACASASIARLKNLNRRTVDVLASRLYSYYSLSYQLTGNLAEIRGNLL
Sbjct: 121 IFLIDQKKYDEAVACASASIARLKNLNRRTVDVLASRLYSYYSLSYQLTGNLAEIRGNLL 180

Query: 181 ALHRIATLRHDELGQETLLNLLLFNYLHYNLYDQAEKLRSKAPRFEAHSNQQASLSALFC 240
           ALHRI TLRHDELGQETLLNLLLFNYLHYNLYDQAEKLRSKAPRFEAHSNQQ      FC
Sbjct: 181 ALHRITTLRHDELGQETLLNLLLFNYLHYNLYDQAEKLRSKAPRFEAHSNQQ------FC 240

Query: 241 RYLFYLGKIRTIQLEYTDAKESLLQAARKAPTAARGFRVQCNKWAIIVRLLLGEIPERTV 300
           RYLFYLGKIRTIQLEYTDAKESLLQAARKAPTAARGFRVQCNKWAIIVRLLLGEIPERTV
Sbjct: 241 RYLFYLGKIRTIQLEYTDAKESLLQAARKAPTAARGFRVQCNKWAIIVRLLLGEIPERTV 300

Query: 301 FTQKGMEIALRPYFELTNAVRIGDLERFKTVAEKFSCTFSSDRTNNLIVRLRHNVIRTGI 360
           FTQKGMEIALRPYFELTNAVRIGDLERFKTVAEKFSCTFSSDRTNNLIVRLRHNVIRTGI
Sbjct: 301 FTQKGMEIALRPYFELTNAVRIGDLERFKTVAEKFSCTFSSDRTNNLIVRLRHNVIRTGI 360

Query: 361 HNISLSYSRISLADVAKKLRLNSENPIADAENIVTKAIRDGAIDATVDHANGWMVSKETG 420
           HNISLSYSRISLADVAKKLRLNSENPIADAENIVTKAIRDGAIDATVDHANGWMVSKETG
Sbjct: 361 HNISLSYSRISLADVAKKLRLNSENPIADAENIVTKAIRDGAIDATVDHANGWMVSKETG 420

Query: 421 DIYSTNEPQIALNSRIAFCLNMHNDAVRALRFPSNSQKEKESAEKRRERRQQEKELAKNI 480
           DIYSTNEPQIALNSRIAFCLNMHNDAVRALRFPSNSQKEKESAEKRRERRQQEKELAKNI
Sbjct: 421 DIYSTNEPQIALNSRIAFCLNMHNDAVRALRFPSNSQKEKESAEKRRERRQQEKELAKNI 480

Query: 481 AEGDDDDFLTDPM 494
           AEGDDDDFLTDPM
Sbjct: 481 AEGDDDDFLTDPM 487

BLAST of Carg05716 vs. NCBI nr
Match: XP_023529673.1 (probable 26S proteasome non-ATPase regulatory subunit 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 912.9 bits (2358), Expect = 1.2e-261
Identity = 480/493 (97.36%), Postives = 483/493 (97.97%), Query Frame = 0

Query: 1   MMQDAEMKELPASVSSSTSPSTLQHLKEIVSLIEAGAYAREVRRIFRAIRLTMALRRRLK 60
           MMQDAEMKELPASVSSSTSPSTLQHLKEIVSLIEAG YAREVRRIFRAIRLTMALRRRLK
Sbjct: 1   MMQDAEMKELPASVSSSTSPSTLQHLKEIVSLIEAGGYAREVRRIFRAIRLTMALRRRLK 60

Query: 61  ASVIFSFLNFALPPGSDVHTKLLSFIPKEDDCEMEIDSAAPAAVKLPLPEIEIFCYLIVL 120
           ASV+FSFLNFALPPGSDVHTKL SFIPKEDDCEMEIDSA PAAVKLPLPEIEIFCYLIVL
Sbjct: 61  ASVLFSFLNFALPPGSDVHTKLSSFIPKEDDCEMEIDSAEPAAVKLPLPEIEIFCYLIVL 120

Query: 121 IFLIDQKKYDEAVACASASIARLKNLNRRTVDVLASRLYSYYSLSYQLTGNLAEIRGNLL 180
           IFLIDQKKYDEAVACASASIARLKNLNRRTVDVLASRLYSYYSLSYQLTGNLAEIRGNLL
Sbjct: 121 IFLIDQKKYDEAVACASASIARLKNLNRRTVDVLASRLYSYYSLSYQLTGNLAEIRGNLL 180

Query: 181 ALHRIATLRHDELGQETLLNLLLFNYLHYNLYDQAEKLRSKAPRFEAHSNQQASLSALFC 240
           ALHR ATLRHDELGQETLLNLLLFNYLHYNLYDQAEKLRSKAPRFEAHSNQQ      FC
Sbjct: 181 ALHRTATLRHDELGQETLLNLLLFNYLHYNLYDQAEKLRSKAPRFEAHSNQQ------FC 240

Query: 241 RYLFYLGKIRTIQLEYTDAKESLLQAARKAPTAARGFRVQCNKWAIIVRLLLGEIPERTV 300
           RYLFYLGKIRTIQLEYTDAKESLLQAARKAPTAARGFRVQCNKWAIIVRLLLGEIPERTV
Sbjct: 241 RYLFYLGKIRTIQLEYTDAKESLLQAARKAPTAARGFRVQCNKWAIIVRLLLGEIPERTV 300

Query: 301 FTQKGMEIALRPYFELTNAVRIGDLERFKTVAEKFSCTFSSDRTNNLIVRLRHNVIRTGI 360
           FTQKGMEIALRPYFELTNAVRIGDLERFKTVAEKFSCTFSSDRTNNLIVRLRHNVIRTGI
Sbjct: 301 FTQKGMEIALRPYFELTNAVRIGDLERFKTVAEKFSCTFSSDRTNNLIVRLRHNVIRTGI 360

Query: 361 HNISLSYSRISLADVAKKLRLNSENPIADAENIVTKAIRDGAIDATVDHANGWMVSKETG 420
           HNISLSYSRISLADVAKKLRLNSENPIADAENIVTK+IRDGAIDATVDHANGWMVSKETG
Sbjct: 361 HNISLSYSRISLADVAKKLRLNSENPIADAENIVTKSIRDGAIDATVDHANGWMVSKETG 420

Query: 421 DIYSTNEPQIALNSRIAFCLNMHNDAVRALRFPSNSQKEKESAEKRRERRQQEKELAKNI 480
           DIYSTNEPQIALNSRIAFCLNMHNDAVRALRFPSNSQKEKESAE+RRERRQQEKELAKNI
Sbjct: 421 DIYSTNEPQIALNSRIAFCLNMHNDAVRALRFPSNSQKEKESAEERRERRQQEKELAKNI 480

Query: 481 AEGDDDDFLTDPM 494
           AEGDDDDFLTDPM
Sbjct: 481 AEGDDDDFLTDPM 487

BLAST of Carg05716 vs. NCBI nr
Match: XP_022989253.1 (probable 26S proteasome non-ATPase regulatory subunit 3 [Cucurbita maxima])

HSP 1 Score: 903.3 bits (2333), Expect = 9.3e-259
Identity = 481/498 (96.59%), Postives = 483/498 (96.99%), Query Frame = 0

Query: 1   MMQDAEMKELPASVSS----STSPSTLQHLKEIVSLIEAGAYAREVRRIFRAIRLTMALR 60
           MMQDAEMKELPASVSS    STSPSTLQHLKEIVSLIEAGAYAREVRR+FRAIRLTMALR
Sbjct: 1   MMQDAEMKELPASVSSSTSPSTSPSTLQHLKEIVSLIEAGAYAREVRRVFRAIRLTMALR 60

Query: 61  RRLKASVIFSFLNFALPPGSDVHTKLLSFIPKEDDCEMEIDSAAPAAVKLPLP-EIEIFC 120
           RRLKASVIFSFLNFALPPGSDVHTKL SFIPKEDDCEMEIDSA+PAA KLPLP EIEIFC
Sbjct: 61  RRLKASVIFSFLNFALPPGSDVHTKLSSFIPKEDDCEMEIDSASPAAAKLPLPAEIEIFC 120

Query: 121 YLIVLIFLIDQKKYDEAVACASASIARLKNLNRRTVDVLASRLYSYYSLSYQLTGNLAEI 180
           YLIVLIFLIDQKKYDEAVACASASIARLKNLNRRTVDVLASRLYSYYSLSYQLTGNLAEI
Sbjct: 121 YLIVLIFLIDQKKYDEAVACASASIARLKNLNRRTVDVLASRLYSYYSLSYQLTGNLAEI 180

Query: 181 RGNLLALHRIATLRHDELGQETLLNLLLFNYLHYNLYDQAEKLRSKAPRFEAHSNQQASL 240
           RGNLLALHR ATLR DELGQETLLNLLLFNYLHYNLYDQAEKLRSKAPRFEAHSNQQ   
Sbjct: 181 RGNLLALHRTATLRRDELGQETLLNLLLFNYLHYNLYDQAEKLRSKAPRFEAHSNQQ--- 240

Query: 241 SALFCRYLFYLGKIRTIQLEYTDAKESLLQAARKAPTAARGFRVQCNKWAIIVRLLLGEI 300
              FCRYLFYLGKIRTIQLEYTDAKESLLQAARKAPTAARGFRVQCNKWAIIVRLLLGEI
Sbjct: 241 ---FCRYLFYLGKIRTIQLEYTDAKESLLQAARKAPTAARGFRVQCNKWAIIVRLLLGEI 300

Query: 301 PERTVFTQKGMEIALRPYFELTNAVRIGDLERFKTVAEKFSCTFSSDRTNNLIVRLRHNV 360
           PERTVFTQKGMEIALRPYFELTNAVRIGDLERFKTVAEKFSCTFSSDRTNNLIVRLRHNV
Sbjct: 301 PERTVFTQKGMEIALRPYFELTNAVRIGDLERFKTVAEKFSCTFSSDRTNNLIVRLRHNV 360

Query: 361 IRTGIHNISLSYSRISLADVAKKLRLNSENPIADAENIVTKAIRDGAIDATVDHANGWMV 420
           IRTGIHNISLSYSRISLADVAKKLRLNSENPIADAENIVTKAIRDGAIDATVDHANGWMV
Sbjct: 361 IRTGIHNISLSYSRISLADVAKKLRLNSENPIADAENIVTKAIRDGAIDATVDHANGWMV 420

Query: 421 SKETGDIYSTNEPQIALNSRIAFCLNMHNDAVRALRFPSNSQKEKESAEKRRERRQQEKE 480
           SKETGDIYSTNEPQIALNSRIAFCLNMHNDAVRALRFPSNSQKEKESAEKRRERRQQEKE
Sbjct: 421 SKETGDIYSTNEPQIALNSRIAFCLNMHNDAVRALRFPSNSQKEKESAEKRRERRQQEKE 480

Query: 481 LAKNIAEGDDDDFLTDPM 494
           LAKNIAEGDDDDFLTDPM
Sbjct: 481 LAKNIAEGDDDDFLTDPM 492

BLAST of Carg05716 vs. NCBI nr
Match: KAG6589020.1 (putative 26S proteasome non-ATPase regulatory subunit 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 880.6 bits (2274), Expect = 6.5e-252
Identity = 469/493 (95.13%), Postives = 470/493 (95.33%), Query Frame = 0

Query: 1   MMQDAEMKELPASVSSSTSPSTLQHLKEIVSLIEAGAYAREVRRIFRAIRLTMALRRRLK 60
           MMQDAEMKELPASV SSTSPSTLQHLKEIVSLIEAGAYAREVRRIFRAIRLTMALRRRLK
Sbjct: 1   MMQDAEMKELPASVYSSTSPSTLQHLKEIVSLIEAGAYAREVRRIFRAIRLTMALRRRLK 60

Query: 61  ASVIFSFLNFALPPGSDVHTKLLSFIPKEDDCEMEIDSAAPAAVKLPLPEIEIFCYLIVL 120
           ASVIFSFLNFALPPGSDVHTKLLSFIPKEDDCEMEIDSAAPAAVKLPLPEIEIFCYLIVL
Sbjct: 61  ASVIFSFLNFALPPGSDVHTKLLSFIPKEDDCEMEIDSAAPAAVKLPLPEIEIFCYLIVL 120

Query: 121 IFLIDQKKYDEAVACASASIARLKNLNRRTVDVLASRLYSYYSLSYQLTGNLAEIRGNLL 180
           IFLIDQKKYDEAVACASASIARLKNLNRRTVDVLASRLYSYYSLSYQLTGNLAEIRGNLL
Sbjct: 121 IFLIDQKKYDEAVACASASIARLKNLNRRTVDVLASRLYSYYSLSYQLTGNLAEIRGNLL 180

Query: 181 ALHRIATLRHDELGQETLLNLLLFNYLHYNLYDQAEKLRSKAPRFEAHSNQQASLSALFC 240
           ALHRIATLRHDELGQETLLNLLLFNYLHYNLYDQAEKL                      
Sbjct: 181 ALHRIATLRHDELGQETLLNLLLFNYLHYNLYDQAEKL---------------------- 240

Query: 241 RYLFYLGKIRTIQLEYTDAKESLLQAARKAPTAARGFRVQCNKWAIIVRLLLGEIPERTV 300
           RYLFYLGKIRTIQLEYTDAKESLLQAARKAPTAARGFRVQCNKWAIIVRLLLGEIPERTV
Sbjct: 241 RYLFYLGKIRTIQLEYTDAKESLLQAARKAPTAARGFRVQCNKWAIIVRLLLGEIPERTV 300

Query: 301 FTQKGMEIALRPYFELTNAVRIGDLERFKTVAEKFSCTFSSDRTNNLIVRLRHNVIRTGI 360
           FTQKGMEIALRPYFELTNAVRIGDLERFKTVAEKFSCTFSSDRTNNLIVRLRHNVIRTGI
Sbjct: 301 FTQKGMEIALRPYFELTNAVRIGDLERFKTVAEKFSCTFSSDRTNNLIVRLRHNVIRTGI 360

Query: 361 HNISLSYSRISLADVAKKLRLNSENPIADAENIVTKAIRDGAIDATVDHANGWMVSKETG 420
           HNISLSYSRISLADVAKKLRLNSENPIADAENIVTKAIRDGAIDATVDHANGWMVSKETG
Sbjct: 361 HNISLSYSRISLADVAKKLRLNSENPIADAENIVTKAIRDGAIDATVDHANGWMVSKETG 420

Query: 421 DIYSTNEPQIALNSRIAFCLNMHNDAVRALRFPSNSQKEKESAEKRRERRQQEKELAKNI 480
           DIYSTNEPQIALNSRIAFCLNMHNDAVRALRFPSNSQKEKESAEKRRERRQQEKELAKNI
Sbjct: 421 DIYSTNEPQIALNSRIAFCLNMHNDAVRALRFPSNSQKEKESAEKRRERRQQEKELAKNI 471

Query: 481 AEGDDDDFLTDPM 494
           AEGDDDDFLTDP+
Sbjct: 481 AEGDDDDFLTDPI 471

BLAST of Carg05716 vs. ExPASy Swiss-Prot
Match: P93768 (Probable 26S proteasome non-ATPase regulatory subunit 3 OS=Nicotiana tabacum OX=4097 GN=21D7 PE=2 SV=1)

HSP 1 Score: 753.8 bits (1945), Expect = 1.2e-216
Identity = 402/494 (81.38%), Postives = 440/494 (89.07%), Query Frame = 0

Query: 1   MMQDAEMKEL---PASVSSSTSPSTLQHLKEIVSLIEAGAYAREVRRIFRAIRLTMALRR 60
           M QD EMKE    P++  SST+PS   HLKEI SLIE GAYAREVRRI RA+RLTMALR+
Sbjct: 1   MTQDVEMKEQAAPPSNSLSSTAPSIFHHLKEIASLIETGAYAREVRRISRAVRLTMALRK 60

Query: 61  RLKASVIFSFLNFALPPGSDVHTKLLSFIPKEDDCEMEIDSA---APAAVKLPLPEIEIF 120
           +LKAS + +FLN+ L PGS+VH++L SF+PKED+ +ME+D+A   A A +K PLPE+EI+
Sbjct: 61  KLKASSLSAFLNYVLVPGSEVHSRLSSFLPKEDEQDMEVDTATSGAQAPIKNPLPELEIY 120

Query: 121 CYLIVLIFLIDQKKYDEAVACASASIARLKNLNRRTVDVLASRLYSYYSLSYQLTGNLAE 180
           CYL+VLIFLIDQKKY+EA AC+SASIARLK +NRRTVDVLASRLY YYSL Y+LTG+LAE
Sbjct: 121 CYLLVLIFLIDQKKYNEAKACSSASIARLKTVNRRTVDVLASRLYFYYSLCYELTGDLAE 180

Query: 181 IRGNLLALHRIATLRHDELGQETLLNLLLFNYLHYNLYDQAEKLRSKAPRFEAHSNQQAS 240
           IRG LLALHRIATLRHDELGQETLLNLLL NYLHYNLYDQAEKLRSKAPRFEAHSNQQ  
Sbjct: 181 IRGYLLALHRIATLRHDELGQETLLNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQ-- 240

Query: 241 LSALFCRYLFYLGKIRTIQLEYTDAKESLLQAARKAPTAARGFRVQCNKWAIIVRLLLGE 300
               F RYLFYLGKIRTIQLEYTDAKESLLQAARKAP AA GFRVQCNKWAIIVRLLLGE
Sbjct: 241 ----FSRYLFYLGKIRTIQLEYTDAKESLLQAARKAPQAALGFRVQCNKWAIIVRLLLGE 300

Query: 301 IPERTVFTQKGMEIALRPYFELTNAVRIGDLERFKTVAEKFSCTFSSDRTNNLIVRLRHN 360
           IPERTVF QKGME ALRPYFELTNAVRIGDLE F+ VAEKFS TFSSD TNNLIVRLRHN
Sbjct: 301 IPERTVFMQKGMEKALRPYFELTNAVRIGDLELFRKVAEKFSSTFSSDGTNNLIVRLRHN 360

Query: 361 VIRTGIHNISLSYSRISLADVAKKLRLNSENPIADAENIVTKAIRDGAIDATVDHANGWM 420
           VIRTG+ NIS+SYSRISL DVAKKLRL+S NP+ADAE+IV+KAIRDGAIDAT+DHANGWM
Sbjct: 361 VIRTGLRNISISYSRISLVDVAKKLRLDSPNPVADAESIVSKAIRDGAIDATLDHANGWM 420

Query: 421 VSKETGDIYSTNEPQIALNSRIAFCLNMHNDAVRALRFPSNSQKEKESAEKRRERRQQEK 480
           VSKETGDIYSTNEPQIA NSRIAFCLNMHN+AVRALRFP NS KEKESAEKRRER+QQE+
Sbjct: 421 VSKETGDIYSTNEPQIAFNSRIAFCLNMHNEAVRALRFPPNSHKEKESAEKRRERQQQEQ 480

Query: 481 ELAKNIAEGDDDDF 489
           ELAK+IAE DDDDF
Sbjct: 481 ELAKHIAEEDDDDF 488

BLAST of Carg05716 vs. ExPASy Swiss-Prot
Match: Q06364 (Probable 26S proteasome non-ATPase regulatory subunit 3 OS=Daucus carota OX=4039 GN=21D7 PE=1 SV=2)

HSP 1 Score: 751.1 bits (1938), Expect = 7.8e-216
Identity = 393/495 (79.39%), Postives = 443/495 (89.49%), Query Frame = 0

Query: 1   MMQDAEMKELPASVSSST----SPSTLQHLKEIVSLIEAGAYAREVRRIFRAIRLTMALR 60
           M QD EMKE+PA   S++    +PSTLQHLKEI SLIE+GAYAREVRRI RA+RLT+ALR
Sbjct: 1   MTQDVEMKEVPAPAPSNSVTAATPSTLQHLKEIASLIESGAYAREVRRILRAVRLTIALR 60

Query: 61  RRLKASVIFSFLNFALPPGSDVHTKLLSFIPKEDDCEMEIDSAAPAAVKL---PLPEIEI 120
           ++L ASV+ +FLNF+L PGS+VH +L S++PKED+ +ME+D+A  A   L    LPE+EI
Sbjct: 61  KKLNASVVNAFLNFSLVPGSEVHARLASYLPKEDEHDMEVDTAMSATTTLAKHSLPELEI 120

Query: 121 FCYLIVLIFLIDQKKYDEAVACASASIARLKNLNRRTVDVLASRLYSYYSLSYQLTGNLA 180
           +CYL+VLIFLIDQKKY EA AC+SASIAR+KNLNRRTV+VLASRLY YYSLSY+LTG+LA
Sbjct: 121 YCYLLVLIFLIDQKKYSEAKACSSASIARVKNLNRRTVEVLASRLYFYYSLSYELTGDLA 180

Query: 181 EIRGNLLALHRIATLRHDELGQETLLNLLLFNYLHYNLYDQAEKLRSKAPRFEAHSNQQA 240
           EIRGNLLALHRIATLRHDELGQETLLNLLL NYLHYNLYDQAEKLRSKAPRFEAHSNQQ 
Sbjct: 181 EIRGNLLALHRIATLRHDELGQETLLNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQ- 240

Query: 241 SLSALFCRYLFYLGKIRTIQLEYTDAKESLLQAARKAPTAARGFRVQCNKWAIIVRLLLG 300
                FCRYLFYLGKIRTIQLEYTDAKES LQAARKAP AA GFRVQCNKWA+IVRLLLG
Sbjct: 241 -----FCRYLFYLGKIRTIQLEYTDAKESXLQAARKAPVAALGFRVQCNKWAVIVRLLLG 300

Query: 301 EIPERTVFTQKGMEIALRPYFELTNAVRIGDLERFKTVAEKFSCTFSSDRTNNLIVRLRH 360
           EIPERTVF QKGME ALRPYF LTNAVRIGDLE F+ VA+KF+ TF++DRT+NLIVRLRH
Sbjct: 301 EIPERTVFMQKGMEKALRPYFXLTNAVRIGDLELFRXVADKFASTFTADRTHNLIVRLRH 360

Query: 361 NVIRTGIHNISLSYSRISLADVAKKLRLNSENPIADAENIVTKAIRDGAIDATVDHANGW 420
           NVIRTG+ NIS+SYSRISL DVA+KLRL+S NP+ADAE+IV+KAIRDGAIDAT+DHANGW
Sbjct: 361 NVIRTGLRNISISYSRISLVDVARKLRLDSPNPVADAESIVSKAIRDGAIDATIDHANGW 420

Query: 421 MVSKETGDIYSTNEPQIALNSRIAFCLNMHNDAVRALRFPSNSQKEKESAEKRRERRQQE 480
           MVSKETGDIYSTNEPQ A NSRIAFCLNMHN+AVRALRFP+NS K+KESAEKRRER+QQE
Sbjct: 421 MVSKETGDIYSTNEPQAAFNSRIAFCLNMHNEAVRALRFPANSHKDKESAEKRRERQQQE 480

Query: 481 KELAKNIAEGDDDDF 489
           +ELAK+IAE DDD+F
Sbjct: 481 QELAKHIAEEDDDEF 489

BLAST of Carg05716 vs. ExPASy Swiss-Prot
Match: Q9LNU4 (26S proteasome non-ATPase regulatory subunit 3 homolog A OS=Arabidopsis thaliana OX=3702 GN=RPN3A PE=1 SV=3)

HSP 1 Score: 713.0 bits (1839), Expect = 2.3e-204
Identity = 377/494 (76.32%), Postives = 429/494 (86.84%), Query Frame = 0

Query: 1   MMQDAEMKE--LPASVSSSTSPSTLQHLKEIVSLIEAGAYAREVRRIFRAIRLTMALRRR 60
           M QD EMK+   P+    S+S ST+Q+LKEI +LI+ G+Y +EVRRI RA+RLT+ LR++
Sbjct: 1   MTQDVEMKDNNTPSQSIISSSTSTMQNLKEIAALIDTGSYTKEVRRIARAVRLTIGLRQK 60

Query: 61  LKASVIFSFLNFALPPGSDVHTKLLSFIPKEDDCEMEIDSAAPAAVKLP---LP-EIEIF 120
           L  SV+ SFL+FAL PGS+ H++L SF+PK D+ +ME+D+A+ A    P   LP E+EI+
Sbjct: 61  LTGSVLSSFLDFALVPGSEAHSRLSSFVPKGDEHDMEVDTASSATQAAPSKHLPAELEIY 120

Query: 121 CYLIVLIFLIDQKKYDEAVACASASIARLKNLNRRTVDVLASRLYSYYSLSYQLTGNLAE 180
           CY IVL+FLIDQKKY+EA AC+SASIARLKN+NRRT+DV+ASRLY YYSLSY+ TG+LAE
Sbjct: 121 CYFIVLLFLIDQKKYNEAKACSSASIARLKNVNRRTIDVIASRLYFYYSLSYEQTGDLAE 180

Query: 181 IRGNLLALHRIATLRHDELGQETLLNLLLFNYLHYNLYDQAEKLRSKAPRFEAHSNQQAS 240
           IRG LLALH  ATLRHDELGQETLLNLLL NYLHYNLYDQAEKLRSKAPRFEAHSNQQ  
Sbjct: 181 IRGTLLALHHSATLRHDELGQETLLNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQ-- 240

Query: 241 LSALFCRYLFYLGKIRTIQLEYTDAKESLLQAARKAPTAARGFRVQCNKWAIIVRLLLGE 300
               FCRYLFYLGKIRTIQLEYTDAKESLLQAARKAP AA GFR+QCNKWAI+VRLLLGE
Sbjct: 241 ----FCRYLFYLGKIRTIQLEYTDAKESLLQAARKAPIAALGFRIQCNKWAILVRLLLGE 300

Query: 301 IPERTVFTQKGMEIALRPYFELTNAVRIGDLERFKTVAEKFSCTFSSDRTNNLIVRLRHN 360
           IPER++FTQKGME ALRPYFELTNAVRIGDLE F+TV EKF  TF+ DRT+NLIVRLRHN
Sbjct: 301 IPERSIFTQKGMEKALRPYFELTNAVRIGDLELFRTVQEKFLDTFAQDRTHNLIVRLRHN 360

Query: 361 VIRTGIHNISLSYSRISLADVAKKLRLNSENPIADAENIVTKAIRDGAIDATVDHANGWM 420
           VIRTG+ NIS+SYSRISL DVAKKLRLNSENP+ADAE+IV KAIRDGAIDAT+DH NG M
Sbjct: 361 VIRTGLRNISISYSRISLPDVAKKLRLNSENPVADAESIVAKAIRDGAIDATIDHKNGCM 420

Query: 421 VSKETGDIYSTNEPQIALNSRIAFCLNMHNDAVRALRFPSNSQKEKESAEKRRERRQQEK 480
           VSKETGDIYSTNEPQ A NSRIAFCLNMHN+AVRALRFP N+ KEKES EKRRER+QQE+
Sbjct: 421 VSKETGDIYSTNEPQTAFNSRIAFCLNMHNEAVRALRFPPNTHKEKESDEKRRERKQQEE 480

Query: 481 ELAKNIAEGDDDDF 489
           ELAK++AE DDDDF
Sbjct: 481 ELAKHMAEEDDDDF 488

BLAST of Carg05716 vs. ExPASy Swiss-Prot
Match: Q9LQR8 (26S proteasome non-ATPase regulatory subunit 3 homolog B OS=Arabidopsis thaliana OX=3702 GN=RPN3B PE=1 SV=2)

HSP 1 Score: 693.0 bits (1787), Expect = 2.5e-198
Identity = 368/493 (74.65%), Postives = 419/493 (84.99%), Query Frame = 0

Query: 1   MMQDAEMK--ELPASVSSSTSPSTLQHLKEIVSLIEAGAYAREVRRIFRAIRLTMALRRR 60
           M QD EMK  + P     S   STLQ+LKEI +LI+ G+Y +EVRRI RA+RLT+ LRR+
Sbjct: 1   MTQDVEMKDNQTPTQSVVSAPTSTLQNLKEIAALIDTGSYTKEVRRIARAVRLTVGLRRK 60

Query: 61  LKASVIFSFLNFALPPGSDVHTKLLSFIPKEDDCEMEIDSAAPAAVKLP--LP-EIEIFC 120
           L  SVI SFL+FAL PGS+ HT+L SF+PK D+ +ME+D+A+  +   P  LP E+EI+C
Sbjct: 61  LTGSVISSFLDFALVPGSEAHTRLSSFVPKSDEHDMEVDTASSNSQAPPKHLPAELEIYC 120

Query: 121 YLIVLIFLIDQKKYDEAVACASASIARLKNLNRRTVDVLASRLYSYYSLSYQLTGNLAEI 180
           Y IVL+FLIDQKKY+EA  C++ASIARLK++NRRTVDV+AS+LY YYSLSY+LT +LAEI
Sbjct: 121 YFIVLLFLIDQKKYNEAKVCSTASIARLKSVNRRTVDVIASKLYFYYSLSYELTNDLAEI 180

Query: 181 RGNLLALHRIATLRHDELGQETLLNLLLFNYLHYNLYDQAEKLRSKAPRFEAHSNQQASL 240
           R  LLALH  ATLRHDELGQETLLNLLL NYLHYNLYDQAEKLRSKAPRFEAHSNQQ   
Sbjct: 181 RSTLLALHHSATLRHDELGQETLLNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQ--- 240

Query: 241 SALFCRYLFYLGKIRTIQLEYTDAKESLLQAARKAPTAARGFRVQCNKWAIIVRLLLGEI 300
              FCRYLFYLGKIRTIQLEYTDAKESLLQAARKAP A+ GFR+QCNKWAIIVRLLLGEI
Sbjct: 241 ---FCRYLFYLGKIRTIQLEYTDAKESLLQAARKAPVASLGFRIQCNKWAIIVRLLLGEI 300

Query: 301 PERTVFTQKGMEIALRPYFELTNAVRIGDLERFKTVAEKFSCTFSSDRTNNLIVRLRHNV 360
           PER++FTQKGME  LRPYFELTNAVRIGDLE F  + EKF+ TF+ DRT+NLIVRLRHNV
Sbjct: 301 PERSIFTQKGMEKTLRPYFELTNAVRIGDLELFGKIQEKFAKTFAEDRTHNLIVRLRHNV 360

Query: 361 IRTGIHNISLSYSRISLADVAKKLRLNSENPIADAENIVTKAIRDGAIDATVDHANGWMV 420
           IRTG+ NIS+SYSRISL DVA+KLRLNS NP+ADAE+IV KAIRDGAIDAT+DH NG MV
Sbjct: 361 IRTGLRNISISYSRISLQDVAQKLRLNSANPVADAESIVAKAIRDGAIDATIDHKNGCMV 420

Query: 421 SKETGDIYSTNEPQIALNSRIAFCLNMHNDAVRALRFPSNSQKEKESAEKRRERRQQEKE 480
           SKETGDIYSTNEPQ A NSRIAFCLNMHN+AVRALRFP N+ +EKES EKRRE +QQE+E
Sbjct: 421 SKETGDIYSTNEPQTAFNSRIAFCLNMHNEAVRALRFPPNTHREKESEEKRREMKQQEEE 480

Query: 481 LAKNIAEGDDDDF 489
           LAK +AE DDDDF
Sbjct: 481 LAKYMAEEDDDDF 487

BLAST of Carg05716 vs. ExPASy Swiss-Prot
Match: P14685 (26S proteasome non-ATPase regulatory subunit 3 OS=Mus musculus OX=10090 GN=Psmd3 PE=1 SV=3)

HSP 1 Score: 369.8 bits (948), Expect = 4.9e-101
Identity = 226/508 (44.49%), Postives = 317/508 (62.40%), Query Frame = 0

Query: 3   QDAEMKELPASVSSSTSPS-----------------TLQHLKEIVSLIEAGAYAREVRRI 62
           QD EMKE  A+ S ST                    TL+ +KE V  +E     +E R +
Sbjct: 31  QDVEMKEEAAAGSGSTGEGDGKAAATEHSQRELDTVTLEDIKEHVRQLEKAVSGKEPRFV 90

Query: 63  FRAIRLTMALRRRLKASVIFSFLNFALPPGSDVHTKLLSFI--PKEDDCEMEIDSAAPAA 122
            RA+R+  +  RRL   V++  ++      +     LL F+  P + + +++       A
Sbjct: 91  LRALRMLPSTSRRLNHYVLYKAVHGFFTSNNATRDFLLPFLEEPMDTEADLQFRPRTGKA 150

Query: 123 VKLP-LPEIEIFCYLIVLIFLIDQKKYDEAVACASASIARLKNLNRRTVDVLASRLYSYY 182
              P LPE+E +  L+++IFL++ K+Y EA   +   + ++   NRR +D++A++ Y Y+
Sbjct: 151 ASAPLLPEVEAYLQLLMVIFLMNSKRYKEAQKISDDLMQKISTQNRRALDLVAAKCYYYH 210

Query: 183 SLSYQLTGNLAEIRGNLLALHRIATLRHDELGQETLLNLLLFNYLHYNLYDQAEKLRSKA 242
           +  Y+    L  +R  L A  R ATLRHD  GQ TLLNLLL NYLHY+LYDQAEKL SK+
Sbjct: 211 ARVYEFLDKLDVVRSFLHARLRTATLRHDADGQATLLNLLLRNYLHYSLYDQAEKLVSKS 270

Query: 243 PRFEAHSNQQASLSALFCRYLFYLGKIRTIQLEYTDAKESLLQAARKAPT-AARGFRVQC 302
              E  +N +      + RYL+Y G+I+ IQLEY++A+ ++  A RKAP   A GF+   
Sbjct: 271 VFPEQANNNE------WARYLYYTGRIKAIQLEYSEARRTMTNALRKAPQHTAVGFKQTV 330

Query: 303 NKWAIIVRLLLGEIPERTVFTQKGMEIALRPYFELTNAVRIGDLERFKTVAEKFSCTFSS 362
           +K  I+V LLLGEIP+R  F Q  ++ +L PYF LT AVR G+L +F  V ++F   F +
Sbjct: 331 HKLLIVVELLLGEIPDRLQFRQPSLKRSLMPYFLLTQAVRTGNLAKFNQVLDQFGEKFQT 390

Query: 363 DRTNNLIVRLRHNVIRTGIHNISLSYSRISLADVAKKLRLNSENPIADAENIVTKAIRDG 422
           D T  LI+RLRHNVI+TG+  ISLSYSRISLAD+A+KL+L+S     DAE IV KAIRDG
Sbjct: 391 DGTYTLIIRLRHNVIKTGVRMISLSYSRISLADIAQKLQLDSPE---DAEFIVAKAIRDG 450

Query: 423 AIDATVDHANGWMVSKETGDIYSTNEPQIALNSRIAFCLNMHNDAVRALRFPSNS-QKEK 482
            I+A+++H  G++ SKE  DIYST EPQ+A + RI+FCL++HN +V+A+RFP  S  K+ 
Sbjct: 451 VIEASINHEKGYVQSKEMIDIYSTREPQLAFHQRISFCLDIHNMSVKAMRFPPKSYNKDL 510

Query: 483 ESAEKRRERRQQEKELAKNIAEGDDDDF 489
           ESAE+RRER QQ+ E AK +AE DDD F
Sbjct: 511 ESAEERREREQQDLEFAKEMAEDDDDSF 529

BLAST of Carg05716 vs. ExPASy TrEMBL
Match: A0A6J1EJV5 (probable 26S proteasome non-ATPase regulatory subunit 3 OS=Cucurbita moschata OX=3662 GN=LOC111435152 PE=3 SV=1)

HSP 1 Score: 917.9 bits (2371), Expect = 1.8e-263
Identity = 484/493 (98.17%), Postives = 484/493 (98.17%), Query Frame = 0

Query: 1   MMQDAEMKELPASVSSSTSPSTLQHLKEIVSLIEAGAYAREVRRIFRAIRLTMALRRRLK 60
           MM DAEMKELPASVSSSTSPSTLQHLKEIVSLIEAGAYAREVRRIFRAIRLTMALRRRLK
Sbjct: 1   MMPDAEMKELPASVSSSTSPSTLQHLKEIVSLIEAGAYAREVRRIFRAIRLTMALRRRLK 60

Query: 61  ASVIFSFLNFALPPGSDVHTKLLSFIPKEDDCEMEIDSAAPAAVKLPLPEIEIFCYLIVL 120
           ASVIFSFLNFALPPGSDVHTKL SFIPKEDDCEMEIDSAAPAAVKLPLPEIEIFCYLIVL
Sbjct: 61  ASVIFSFLNFALPPGSDVHTKLSSFIPKEDDCEMEIDSAAPAAVKLPLPEIEIFCYLIVL 120

Query: 121 IFLIDQKKYDEAVACASASIARLKNLNRRTVDVLASRLYSYYSLSYQLTGNLAEIRGNLL 180
           IFLIDQKKYDEAVACASASIARLKNLNRRTVDVLASRLYSYYSLSYQLTGNLAEIRGNLL
Sbjct: 121 IFLIDQKKYDEAVACASASIARLKNLNRRTVDVLASRLYSYYSLSYQLTGNLAEIRGNLL 180

Query: 181 ALHRIATLRHDELGQETLLNLLLFNYLHYNLYDQAEKLRSKAPRFEAHSNQQASLSALFC 240
           ALHRI TLRHDELGQETLLNLLLFNYLHYNLYDQAEKLRSKAPRFEAHSNQQ      FC
Sbjct: 181 ALHRITTLRHDELGQETLLNLLLFNYLHYNLYDQAEKLRSKAPRFEAHSNQQ------FC 240

Query: 241 RYLFYLGKIRTIQLEYTDAKESLLQAARKAPTAARGFRVQCNKWAIIVRLLLGEIPERTV 300
           RYLFYLGKIRTIQLEYTDAKESLLQAARKAPTAARGFRVQCNKWAIIVRLLLGEIPERTV
Sbjct: 241 RYLFYLGKIRTIQLEYTDAKESLLQAARKAPTAARGFRVQCNKWAIIVRLLLGEIPERTV 300

Query: 301 FTQKGMEIALRPYFELTNAVRIGDLERFKTVAEKFSCTFSSDRTNNLIVRLRHNVIRTGI 360
           FTQKGMEIALRPYFELTNAVRIGDLERFKTVAEKFSCTFSSDRTNNLIVRLRHNVIRTGI
Sbjct: 301 FTQKGMEIALRPYFELTNAVRIGDLERFKTVAEKFSCTFSSDRTNNLIVRLRHNVIRTGI 360

Query: 361 HNISLSYSRISLADVAKKLRLNSENPIADAENIVTKAIRDGAIDATVDHANGWMVSKETG 420
           HNISLSYSRISLADVAKKLRLNSENPIADAENIVTKAIRDGAIDATVDHANGWMVSKETG
Sbjct: 361 HNISLSYSRISLADVAKKLRLNSENPIADAENIVTKAIRDGAIDATVDHANGWMVSKETG 420

Query: 421 DIYSTNEPQIALNSRIAFCLNMHNDAVRALRFPSNSQKEKESAEKRRERRQQEKELAKNI 480
           DIYSTNEPQIALNSRIAFCLNMHNDAVRALRFPSNSQKEKESAEKRRERRQQEKELAKNI
Sbjct: 421 DIYSTNEPQIALNSRIAFCLNMHNDAVRALRFPSNSQKEKESAEKRRERRQQEKELAKNI 480

Query: 481 AEGDDDDFLTDPM 494
           AEGDDDDFLTDPM
Sbjct: 481 AEGDDDDFLTDPM 487

BLAST of Carg05716 vs. ExPASy TrEMBL
Match: A0A6J1JPR5 (probable 26S proteasome non-ATPase regulatory subunit 3 OS=Cucurbita maxima OX=3661 GN=LOC111486382 PE=3 SV=1)

HSP 1 Score: 903.3 bits (2333), Expect = 4.5e-259
Identity = 481/498 (96.59%), Postives = 483/498 (96.99%), Query Frame = 0

Query: 1   MMQDAEMKELPASVSS----STSPSTLQHLKEIVSLIEAGAYAREVRRIFRAIRLTMALR 60
           MMQDAEMKELPASVSS    STSPSTLQHLKEIVSLIEAGAYAREVRR+FRAIRLTMALR
Sbjct: 1   MMQDAEMKELPASVSSSTSPSTSPSTLQHLKEIVSLIEAGAYAREVRRVFRAIRLTMALR 60

Query: 61  RRLKASVIFSFLNFALPPGSDVHTKLLSFIPKEDDCEMEIDSAAPAAVKLPLP-EIEIFC 120
           RRLKASVIFSFLNFALPPGSDVHTKL SFIPKEDDCEMEIDSA+PAA KLPLP EIEIFC
Sbjct: 61  RRLKASVIFSFLNFALPPGSDVHTKLSSFIPKEDDCEMEIDSASPAAAKLPLPAEIEIFC 120

Query: 121 YLIVLIFLIDQKKYDEAVACASASIARLKNLNRRTVDVLASRLYSYYSLSYQLTGNLAEI 180
           YLIVLIFLIDQKKYDEAVACASASIARLKNLNRRTVDVLASRLYSYYSLSYQLTGNLAEI
Sbjct: 121 YLIVLIFLIDQKKYDEAVACASASIARLKNLNRRTVDVLASRLYSYYSLSYQLTGNLAEI 180

Query: 181 RGNLLALHRIATLRHDELGQETLLNLLLFNYLHYNLYDQAEKLRSKAPRFEAHSNQQASL 240
           RGNLLALHR ATLR DELGQETLLNLLLFNYLHYNLYDQAEKLRSKAPRFEAHSNQQ   
Sbjct: 181 RGNLLALHRTATLRRDELGQETLLNLLLFNYLHYNLYDQAEKLRSKAPRFEAHSNQQ--- 240

Query: 241 SALFCRYLFYLGKIRTIQLEYTDAKESLLQAARKAPTAARGFRVQCNKWAIIVRLLLGEI 300
              FCRYLFYLGKIRTIQLEYTDAKESLLQAARKAPTAARGFRVQCNKWAIIVRLLLGEI
Sbjct: 241 ---FCRYLFYLGKIRTIQLEYTDAKESLLQAARKAPTAARGFRVQCNKWAIIVRLLLGEI 300

Query: 301 PERTVFTQKGMEIALRPYFELTNAVRIGDLERFKTVAEKFSCTFSSDRTNNLIVRLRHNV 360
           PERTVFTQKGMEIALRPYFELTNAVRIGDLERFKTVAEKFSCTFSSDRTNNLIVRLRHNV
Sbjct: 301 PERTVFTQKGMEIALRPYFELTNAVRIGDLERFKTVAEKFSCTFSSDRTNNLIVRLRHNV 360

Query: 361 IRTGIHNISLSYSRISLADVAKKLRLNSENPIADAENIVTKAIRDGAIDATVDHANGWMV 420
           IRTGIHNISLSYSRISLADVAKKLRLNSENPIADAENIVTKAIRDGAIDATVDHANGWMV
Sbjct: 361 IRTGIHNISLSYSRISLADVAKKLRLNSENPIADAENIVTKAIRDGAIDATVDHANGWMV 420

Query: 421 SKETGDIYSTNEPQIALNSRIAFCLNMHNDAVRALRFPSNSQKEKESAEKRRERRQQEKE 480
           SKETGDIYSTNEPQIALNSRIAFCLNMHNDAVRALRFPSNSQKEKESAEKRRERRQQEKE
Sbjct: 421 SKETGDIYSTNEPQIALNSRIAFCLNMHNDAVRALRFPSNSQKEKESAEKRRERRQQEKE 480

Query: 481 LAKNIAEGDDDDFLTDPM 494
           LAKNIAEGDDDDFLTDPM
Sbjct: 481 LAKNIAEGDDDDFLTDPM 492

BLAST of Carg05716 vs. ExPASy TrEMBL
Match: A0A6J1C6S2 (probable 26S proteasome non-ATPase regulatory subunit 3 OS=Momordica charantia OX=3673 GN=LOC111007897 PE=3 SV=1)

HSP 1 Score: 807.0 bits (2083), Expect = 4.4e-230
Identity = 430/492 (87.40%), Postives = 452/492 (91.87%), Query Frame = 0

Query: 1   MMQDAEMKELPASVSS----STSPSTLQHLKEIVSLIEAGAYAREVRRIFRAIRLTMALR 60
           M QDAEMKELPA +SS    STSPS LQHLKEIVSLIE GAYAREVRRI RAIRLTMALR
Sbjct: 1   MTQDAEMKELPAPISSSSAASTSPSALQHLKEIVSLIETGAYAREVRRIVRAIRLTMALR 60

Query: 61  RRLKASVIFSFLNFALPPGSDVHTKLLSFIPKEDDCEMEIDSAAPAAVKLPLPEIEIFCY 120
           R+LKASV+ SFLN AL PGSDVHT+L SF+PKEDD EM++DSA  A  K PLPEIEIFCY
Sbjct: 61  RKLKASVLSSFLNSALQPGSDVHTRLSSFLPKEDDYEMDVDSATQAPAKHPLPEIEIFCY 120

Query: 121 LIVLIFLIDQKKYDEAVACASASIARLKNLNRRTVDVLASRLYSYYSLSYQLTGNLAEIR 180
           LIVLIFLIDQKKYDEA ACA+ASIARLKNLNRRTVDVLASRLY YYSLSY+LTG+LAEIR
Sbjct: 121 LIVLIFLIDQKKYDEAKACATASIARLKNLNRRTVDVLASRLYFYYSLSYELTGDLAEIR 180

Query: 181 GNLLALHRIATLRHDELGQETLLNLLLFNYLHYNLYDQAEKLRSKAPRFEAHSNQQASLS 240
           GNLLALHRIATLRHDELGQETLLNLLL NYLHYNLYDQAEKLRSKAPRFEAHSNQQ    
Sbjct: 181 GNLLALHRIATLRHDELGQETLLNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQ---- 240

Query: 241 ALFCRYLFYLGKIRTIQLEYTDAKESLLQAARKAPTAARGFRVQCNKWAIIVRLLLGEIP 300
             FCRYLFYLGKIRTIQLEYTDAKESLLQAARKAP AA GFRVQCNKWAIIVRLLLGEIP
Sbjct: 241 --FCRYLFYLGKIRTIQLEYTDAKESLLQAARKAPIAALGFRVQCNKWAIIVRLLLGEIP 300

Query: 301 ERTVFTQKGMEIALRPYFELTNAVRIGDLERFKTVAEKFSCTFSSDRTNNLIVRLRHNVI 360
           ERTVF QKGME ALRPYFELTNAVRIGDLE F+TVAEKFS TFSSDRTNNLIVRLRHNVI
Sbjct: 301 ERTVFMQKGMETALRPYFELTNAVRIGDLELFRTVAEKFSSTFSSDRTNNLIVRLRHNVI 360

Query: 361 RTGIHNISLSYSRISLADVAKKLRLNSENPIADAENIVTKAIRDGAIDATVDHANGWMVS 420
           RT + NIS+SYSRISLADVAKKLRL+SENP+ADAE+IV+KAIRDGAIDATVDHANGWMVS
Sbjct: 361 RTALRNISISYSRISLADVAKKLRLDSENPVADAESIVSKAIRDGAIDATVDHANGWMVS 420

Query: 421 KETGDIYSTNEPQIALNSRIAFCLNMHNDAVRALRFPSNSQKEKESAEKRRERRQQEKEL 480
           KETGDIYSTNEPQ+A NSRIAFCLNMHN+AVRALRFP NS KEKESAEKRRER+QQE+EL
Sbjct: 421 KETGDIYSTNEPQMAFNSRIAFCLNMHNEAVRALRFPPNSHKEKESAEKRRERQQQEQEL 480

Query: 481 AKNIAEGDDDDF 489
           AK+IAE DDDDF
Sbjct: 481 AKHIAEEDDDDF 486

BLAST of Carg05716 vs. ExPASy TrEMBL
Match: A0A6J1GMC8 (probable 26S proteasome non-ATPase regulatory subunit 3 OS=Cucurbita moschata OX=3662 GN=LOC111455623 PE=3 SV=1)

HSP 1 Score: 799.3 bits (2063), Expect = 9.2e-228
Identity = 427/495 (86.26%), Postives = 452/495 (91.31%), Query Frame = 0

Query: 1   MMQDAEMKELPASVSSS---TSPSTLQHLKEIVSLIEAGAYAREVRRIFRAIRLTMALRR 60
           M QDAEMKELP  +SSS   TSPSTLQHLKEIVSL+E GAYAREVRRI RAIRLTMALR+
Sbjct: 1   MTQDAEMKELPPPISSSAAPTSPSTLQHLKEIVSLLETGAYAREVRRIVRAIRLTMALRQ 60

Query: 61  RLKASVIFSFLNFALPPGSDVHTKLLSFIPKEDDCEMEIDS----AAPAAVKLPLPEIEI 120
           +LKASV+  FLNFALPPGSDVHT+L SFIPKEDD EM+IDS       A VK PLPEIEI
Sbjct: 61  KLKASVLSPFLNFALPPGSDVHTRLSSFIPKEDDYEMDIDSETKALTQAPVKHPLPEIEI 120

Query: 121 FCYLIVLIFLIDQKKYDEAVACASASIARLKNLNRRTVDVLASRLYSYYSLSYQLTGNLA 180
           FCYL++L+FLIDQKKY EA ACASASIARLKNLNRRTVDVL+SRLY YYSLSY+LTG+LA
Sbjct: 121 FCYLLLLVFLIDQKKYGEAKACASASIARLKNLNRRTVDVLSSRLYFYYSLSYELTGDLA 180

Query: 181 EIRGNLLALHRIATLRHDELGQETLLNLLLFNYLHYNLYDQAEKLRSKAPRFEAHSNQQA 240
           EIRGNLLALHRIATLRHDELGQETLLNLLL NYLHYNLYDQAEKLRSKAPRFEAHSNQQ 
Sbjct: 181 EIRGNLLALHRIATLRHDELGQETLLNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQ- 240

Query: 241 SLSALFCRYLFYLGKIRTIQLEYTDAKESLLQAARKAPTAARGFRVQCNKWAIIVRLLLG 300
                FCRYLFYLGKIRTIQLEYTDAKESLLQAARKAP AA GFRVQCNKWAIIVRLLLG
Sbjct: 241 -----FCRYLFYLGKIRTIQLEYTDAKESLLQAARKAPIAALGFRVQCNKWAIIVRLLLG 300

Query: 301 EIPERTVFTQKGMEIALRPYFELTNAVRIGDLERFKTVAEKFSCTFSSDRTNNLIVRLRH 360
           EIPERTVF QKGME ALRPYFELTNAVRIGDLE F+TVA+KFS TFSSDRTNNLIVRLRH
Sbjct: 301 EIPERTVFMQKGMETALRPYFELTNAVRIGDLELFRTVADKFSSTFSSDRTNNLIVRLRH 360

Query: 361 NVIRTGIHNISLSYSRISLADVAKKLRLNSENPIADAENIVTKAIRDGAIDATVDHANGW 420
           NVIRTG+ NIS+SYSRISLAD+AKKLRL+SENPIADAE+IV+KAIRDGAIDATVDHA GW
Sbjct: 361 NVIRTGLRNISISYSRISLADIAKKLRLDSENPIADAESIVSKAIRDGAIDATVDHAKGW 420

Query: 421 MVSKETGDIYSTNEPQIALNSRIAFCLNMHNDAVRALRFPSNSQKEKESAEKRRERRQQE 480
           MVSKETGDIYSTNEPQIA NSRIAFCLNMHN+AVRALRFP NS KEKESAEKRRER+QQE
Sbjct: 421 MVSKETGDIYSTNEPQIAFNSRIAFCLNMHNEAVRALRFPPNSHKEKESAEKRRERQQQE 480

Query: 481 KELAKNIAEGDDDDF 489
           +ELAK+IAE DDDDF
Sbjct: 481 QELAKHIAEEDDDDF 489

BLAST of Carg05716 vs. ExPASy TrEMBL
Match: A0A6J1HXW8 (probable 26S proteasome non-ATPase regulatory subunit 3 OS=Cucurbita maxima OX=3661 GN=LOC111468464 PE=3 SV=1)

HSP 1 Score: 797.7 bits (2059), Expect = 2.7e-227
Identity = 426/495 (86.06%), Postives = 452/495 (91.31%), Query Frame = 0

Query: 1   MMQDAEMKELPASVSSS---TSPSTLQHLKEIVSLIEAGAYAREVRRIFRAIRLTMALRR 60
           M QDAEMKELP  +SSS   TSPSTLQHLKEIVSL+E GAYAREVRRI RAIRLTMALR+
Sbjct: 1   MTQDAEMKELPPPISSSAALTSPSTLQHLKEIVSLLETGAYAREVRRIVRAIRLTMALRQ 60

Query: 61  RLKASVIFSFLNFALPPGSDVHTKLLSFIPKEDDCEMEIDS----AAPAAVKLPLPEIEI 120
           +LKASV+  FLNFALPPGSDVHT+L SFIPKEDD EM++DS       A VK PLPEIEI
Sbjct: 61  KLKASVLSPFLNFALPPGSDVHTRLSSFIPKEDDYEMDVDSETKALTQAPVKHPLPEIEI 120

Query: 121 FCYLIVLIFLIDQKKYDEAVACASASIARLKNLNRRTVDVLASRLYSYYSLSYQLTGNLA 180
           FCYL++L+FLIDQKKY EA ACASASIARLKNLNRRTVDVL+SRLY YYSLSY+LTG+LA
Sbjct: 121 FCYLLLLVFLIDQKKYGEAKACASASIARLKNLNRRTVDVLSSRLYFYYSLSYELTGDLA 180

Query: 181 EIRGNLLALHRIATLRHDELGQETLLNLLLFNYLHYNLYDQAEKLRSKAPRFEAHSNQQA 240
           EIRGNLLALHRIATLRHDELGQETLLNLLL NYL+YNLYDQAEKLRSKAPRFEAHSNQQ 
Sbjct: 181 EIRGNLLALHRIATLRHDELGQETLLNLLLRNYLYYNLYDQAEKLRSKAPRFEAHSNQQ- 240

Query: 241 SLSALFCRYLFYLGKIRTIQLEYTDAKESLLQAARKAPTAARGFRVQCNKWAIIVRLLLG 300
                FCRYLFYLGKIRTIQLEYTDAKESLLQAARKAP AA GFRVQCNKWAIIVRLLLG
Sbjct: 241 -----FCRYLFYLGKIRTIQLEYTDAKESLLQAARKAPIAALGFRVQCNKWAIIVRLLLG 300

Query: 301 EIPERTVFTQKGMEIALRPYFELTNAVRIGDLERFKTVAEKFSCTFSSDRTNNLIVRLRH 360
           EIPERTVF QKGME ALRPYFELTNAVRIGDLE F+TVAEKFS TFSSDRTNNLIVRLRH
Sbjct: 301 EIPERTVFMQKGMETALRPYFELTNAVRIGDLELFRTVAEKFSSTFSSDRTNNLIVRLRH 360

Query: 361 NVIRTGIHNISLSYSRISLADVAKKLRLNSENPIADAENIVTKAIRDGAIDATVDHANGW 420
           NVIRTG+ NIS+SYSRISLAD+AKKLRL+SENPIADAE+IV+KAIRDGAIDATVDHA GW
Sbjct: 361 NVIRTGLRNISISYSRISLADIAKKLRLDSENPIADAESIVSKAIRDGAIDATVDHAKGW 420

Query: 421 MVSKETGDIYSTNEPQIALNSRIAFCLNMHNDAVRALRFPSNSQKEKESAEKRRERRQQE 480
           MVSKETGDIYSTNEPQIA NSRIAFCLNMHN+AVRALRFP NS KEKESAEKRRER+QQE
Sbjct: 421 MVSKETGDIYSTNEPQIAFNSRIAFCLNMHNEAVRALRFPPNSHKEKESAEKRRERQQQE 480

Query: 481 KELAKNIAEGDDDDF 489
           +ELAK+IAE DDDDF
Sbjct: 481 QELAKHIAEEDDDDF 489

BLAST of Carg05716 vs. TAIR 10
Match: AT1G20200.1 (PAM domain (PCI/PINT associated module) protein )

HSP 1 Score: 713.0 bits (1839), Expect = 1.7e-205
Identity = 377/494 (76.32%), Postives = 429/494 (86.84%), Query Frame = 0

Query: 1   MMQDAEMKE--LPASVSSSTSPSTLQHLKEIVSLIEAGAYAREVRRIFRAIRLTMALRRR 60
           M QD EMK+   P+    S+S ST+Q+LKEI +LI+ G+Y +EVRRI RA+RLT+ LR++
Sbjct: 1   MTQDVEMKDNNTPSQSIISSSTSTMQNLKEIAALIDTGSYTKEVRRIARAVRLTIGLRQK 60

Query: 61  LKASVIFSFLNFALPPGSDVHTKLLSFIPKEDDCEMEIDSAAPAAVKLP---LP-EIEIF 120
           L  SV+ SFL+FAL PGS+ H++L SF+PK D+ +ME+D+A+ A    P   LP E+EI+
Sbjct: 61  LTGSVLSSFLDFALVPGSEAHSRLSSFVPKGDEHDMEVDTASSATQAAPSKHLPAELEIY 120

Query: 121 CYLIVLIFLIDQKKYDEAVACASASIARLKNLNRRTVDVLASRLYSYYSLSYQLTGNLAE 180
           CY IVL+FLIDQKKY+EA AC+SASIARLKN+NRRT+DV+ASRLY YYSLSY+ TG+LAE
Sbjct: 121 CYFIVLLFLIDQKKYNEAKACSSASIARLKNVNRRTIDVIASRLYFYYSLSYEQTGDLAE 180

Query: 181 IRGNLLALHRIATLRHDELGQETLLNLLLFNYLHYNLYDQAEKLRSKAPRFEAHSNQQAS 240
           IRG LLALH  ATLRHDELGQETLLNLLL NYLHYNLYDQAEKLRSKAPRFEAHSNQQ  
Sbjct: 181 IRGTLLALHHSATLRHDELGQETLLNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQ-- 240

Query: 241 LSALFCRYLFYLGKIRTIQLEYTDAKESLLQAARKAPTAARGFRVQCNKWAIIVRLLLGE 300
               FCRYLFYLGKIRTIQLEYTDAKESLLQAARKAP AA GFR+QCNKWAI+VRLLLGE
Sbjct: 241 ----FCRYLFYLGKIRTIQLEYTDAKESLLQAARKAPIAALGFRIQCNKWAILVRLLLGE 300

Query: 301 IPERTVFTQKGMEIALRPYFELTNAVRIGDLERFKTVAEKFSCTFSSDRTNNLIVRLRHN 360
           IPER++FTQKGME ALRPYFELTNAVRIGDLE F+TV EKF  TF+ DRT+NLIVRLRHN
Sbjct: 301 IPERSIFTQKGMEKALRPYFELTNAVRIGDLELFRTVQEKFLDTFAQDRTHNLIVRLRHN 360

Query: 361 VIRTGIHNISLSYSRISLADVAKKLRLNSENPIADAENIVTKAIRDGAIDATVDHANGWM 420
           VIRTG+ NIS+SYSRISL DVAKKLRLNSENP+ADAE+IV KAIRDGAIDAT+DH NG M
Sbjct: 361 VIRTGLRNISISYSRISLPDVAKKLRLNSENPVADAESIVAKAIRDGAIDATIDHKNGCM 420

Query: 421 VSKETGDIYSTNEPQIALNSRIAFCLNMHNDAVRALRFPSNSQKEKESAEKRRERRQQEK 480
           VSKETGDIYSTNEPQ A NSRIAFCLNMHN+AVRALRFP N+ KEKES EKRRER+QQE+
Sbjct: 421 VSKETGDIYSTNEPQTAFNSRIAFCLNMHNEAVRALRFPPNTHKEKESDEKRRERKQQEE 480

Query: 481 ELAKNIAEGDDDDF 489
           ELAK++AE DDDDF
Sbjct: 481 ELAKHMAEEDDDDF 488

BLAST of Carg05716 vs. TAIR 10
Match: AT1G75990.1 (PAM domain (PCI/PINT associated module) protein )

HSP 1 Score: 693.0 bits (1787), Expect = 1.8e-199
Identity = 368/493 (74.65%), Postives = 419/493 (84.99%), Query Frame = 0

Query: 1   MMQDAEMK--ELPASVSSSTSPSTLQHLKEIVSLIEAGAYAREVRRIFRAIRLTMALRRR 60
           M QD EMK  + P     S   STLQ+LKEI +LI+ G+Y +EVRRI RA+RLT+ LRR+
Sbjct: 1   MTQDVEMKDNQTPTQSVVSAPTSTLQNLKEIAALIDTGSYTKEVRRIARAVRLTVGLRRK 60

Query: 61  LKASVIFSFLNFALPPGSDVHTKLLSFIPKEDDCEMEIDSAAPAAVKLP--LP-EIEIFC 120
           L  SVI SFL+FAL PGS+ HT+L SF+PK D+ +ME+D+A+  +   P  LP E+EI+C
Sbjct: 61  LTGSVISSFLDFALVPGSEAHTRLSSFVPKSDEHDMEVDTASSNSQAPPKHLPAELEIYC 120

Query: 121 YLIVLIFLIDQKKYDEAVACASASIARLKNLNRRTVDVLASRLYSYYSLSYQLTGNLAEI 180
           Y IVL+FLIDQKKY+EA  C++ASIARLK++NRRTVDV+AS+LY YYSLSY+LT +LAEI
Sbjct: 121 YFIVLLFLIDQKKYNEAKVCSTASIARLKSVNRRTVDVIASKLYFYYSLSYELTNDLAEI 180

Query: 181 RGNLLALHRIATLRHDELGQETLLNLLLFNYLHYNLYDQAEKLRSKAPRFEAHSNQQASL 240
           R  LLALH  ATLRHDELGQETLLNLLL NYLHYNLYDQAEKLRSKAPRFEAHSNQQ   
Sbjct: 181 RSTLLALHHSATLRHDELGQETLLNLLLRNYLHYNLYDQAEKLRSKAPRFEAHSNQQ--- 240

Query: 241 SALFCRYLFYLGKIRTIQLEYTDAKESLLQAARKAPTAARGFRVQCNKWAIIVRLLLGEI 300
              FCRYLFYLGKIRTIQLEYTDAKESLLQAARKAP A+ GFR+QCNKWAIIVRLLLGEI
Sbjct: 241 ---FCRYLFYLGKIRTIQLEYTDAKESLLQAARKAPVASLGFRIQCNKWAIIVRLLLGEI 300

Query: 301 PERTVFTQKGMEIALRPYFELTNAVRIGDLERFKTVAEKFSCTFSSDRTNNLIVRLRHNV 360
           PER++FTQKGME  LRPYFELTNAVRIGDLE F  + EKF+ TF+ DRT+NLIVRLRHNV
Sbjct: 301 PERSIFTQKGMEKTLRPYFELTNAVRIGDLELFGKIQEKFAKTFAEDRTHNLIVRLRHNV 360

Query: 361 IRTGIHNISLSYSRISLADVAKKLRLNSENPIADAENIVTKAIRDGAIDATVDHANGWMV 420
           IRTG+ NIS+SYSRISL DVA+KLRLNS NP+ADAE+IV KAIRDGAIDAT+DH NG MV
Sbjct: 361 IRTGLRNISISYSRISLQDVAQKLRLNSANPVADAESIVAKAIRDGAIDATIDHKNGCMV 420

Query: 421 SKETGDIYSTNEPQIALNSRIAFCLNMHNDAVRALRFPSNSQKEKESAEKRRERRQQEKE 480
           SKETGDIYSTNEPQ A NSRIAFCLNMHN+AVRALRFP N+ +EKES EKRRE +QQE+E
Sbjct: 421 SKETGDIYSTNEPQTAFNSRIAFCLNMHNEAVRALRFPPNTHREKESEEKRREMKQQEEE 480

Query: 481 LAKNIAEGDDDDF 489
           LAK +AE DDDDF
Sbjct: 481 LAKYMAEEDDDDF 487