Carg05648 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg05648
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionAutophagy-related protein 18f
LocationCarg_Chr16: 3566404 .. 3570463 (+)
RNA-Seq ExpressionCarg05648
SyntenyCarg05648
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTTTGTCTTCCTTTTATCTATTCCTTTTGTGGGTATTGAGATTTTGTTTACTGGGTTTGCACCCTTTTCGTTGCTTGGTGCAAATGACTCATGATCAGAATTGAGGCGATTTAGTTTCATTGATTTGGGAATTCTCTTCTGTTGATTATGTGAGTTCTTAAAAGGGTCTGATGATTGCTGATGATTGAAGCAGATGGAGAGTTGGAAATTGAATGTGTTGAAATTTAGAGGGGCTGTTTCTTTCCTTTTAGTAATTGCTGGGGATTGACAAGAAACAGGGTGGGGTTATGATTAGAGTGGAATTTGTAGATTATTTTGCACTTCGATTCTGGGGATGGGGAATGATAGAGCTGTAAAACAACAGGGAACCGTAGCGCGCTCTGGGCGTGCTCATGAGTTCATATCAAGTTCGTTTCGTGCTTTTTCTAGTTACATGAAGATTGTTTCTGCTGGTGCTTCGACTGTGGCACGGTCCGCTGCATCAGTGGCATCTTCTTTGGTGGATAAGGAGGATGAAGCAAATGGCTTCCAGGTATGTGGTGGACTTTGATGAAGTTCAAATACTTGGAATTGTCATGAATTGCTGCTTTCTTCATTAAATTTGTTGGAATAATGATGATTTCTGCTGAACCCATATGAATTTTGTGATTTAAGTAGCGTTTTTCAGTGTGTATTTGTAATCTCCTAATAAAAATAAGGTTTTAGTTTCCTTATATTTGAAACAAAGTTGTTTATCTTGCATGAACACAACATGAATGTAAACGTGTTCTGTCATCACGCATGTCCTGCTGGATCTTTTCCAACATTTGCAGCTCTGTTGTGCGATGTAATTGTGTTTGTGGAATGTGTTGTCACTTTATTATGAGCGTTGTAGCCCATCTTTGTACTGTTCTTGATTTGACAAAGGAGTTTTAATCTCTAAGTTCTTGATGGTTTCATAAATTTGGTAATTCTTGCCAATTTTTTGTAGTTCGATCGGTATGATGTTAGCACCTTTTCATTTTGTTCTTGTTTTTCTCTGGATAAATGGGTGATTATTGATTCTTAGTTTCATTGTTATATGATAAAGGTGAACTGGGCTGGTTTTGACAAGTTAGAGTGGGACGACAATGTCGTTCGACGGGTTCTTCTGTTAGGTTATCGTTCGGGTTTCCAGGTTTGGGACGTTGAGGAAGCAAATAATGTCCAAGACCTGGTTTCTCGTTACGATGGTTCTGTTTCATATATGCAAGTGTTGCCCAGACCAATACCGTCGATGAGATCGGGTGATAAGTTTACAGAAAGTAGACCATTGCTGGTGCTTTCTGCTTATGGTTCCATTACTGCTGGTTTTAAAATTCAGGATCGATTAGCAACTTCTGGCAATGCAGCCGTCCCAAAGGTCCAGGAACTAGCCGTTGCCGGTTTTATGCCTACTTTTGTTAGGTTTTATTCCTTGAAATCTCAAACCTACGTGCACGAGCTCAAATTTAGATCGGTTGTTTATTCTGTGAGATGCAGTGCCCTGGTTGTTGCCATTTCTCTCTTTACTCAGGTACGTTTGCTCACTCGAGCAGCTGCAGTCTTATTATTCGTCCTTGTAAAACATTAACCATAAATGTAGGCTGTTTGGTTGGGAATTTGAGTTCTTTTGTCCCACAGATACACTGCATCAATGCTACAACTTTGGAGACAGAACATATCATTCATACAAATCCTGTTGCTTCTGGATTTCTGGGTTCTGGAGGAGGTATCGGTTATGGACCTCTTGCGCTGGGATCCCGGTGGCTGGCTTATAGTGGAAGTCCTATCATTGCGTCGAATGTAGGGCGAGTGACACCACAGCACTTAAAACCTTCTGCTAGTTTATCTCATTCTTCTTTAAATGGAAGTTTAGTTGCTCACTATGCGAAGGAATCGAGTAAGCAACTTGCAGCAGGCCTTGTTACCATCGGTGATAAGGGTATTAAGAAGCTATCGAGATATTACTCGGAGCTCTTGCCCGATAGCAACAATTCTCATCAACATGGAGCTCAGGGTTTGAATGGTATTGGATATCTTAAAGATCATGTGGCTGATGCAGATAGTGTTGGAATGGTCTGTGTTTTCTCTGATTGAGTATTGATTATGCAATTGAATTGATTTAGCTATGATGGTAAAAAGATCTAAAGTGATAACATCTCGTACTTTTTGATATCAGGTTATTGTGAAAGACATTATCAGCAAATCGGTTATCACGCAGTTTCGGGCTCATAAGAGCCCTATTTCTGCTTTATGCTTTGATCCGAGCGGCACCATCTTGGTGACAGCTTCTATTCAGGGTCATAACATAAATGTTTTCAACATTACGCCGAGTTCGAGTTCGAGTTCGAATTCATCTGTGTCTAGTACTGGTGCGTCTTATCGCCATCTCTACAGACTCCAACGAGGCTTCACTAATGCAGTAAGAGGAATGCAATTTGATTTCATTGTTGTGACGTTTTCAGTGTCGTAATCGTGTTTTTCGATCTATTATCAGGTCATACAAGACATCAGTTTCAGCTATGATAGCAACTGGATCATGATTAGTTCGTCCAGAGGGACGAGTCATCTCTTTGCTATAAACCCTGCTGGAGGACAGGTGAATTTCCCATCTACTAGTTCTGCTACCAGAACTGATGGGTTAGTAGTTCCTGCAAGACAAACCGTTTGGTTGGTCGATTCTGGTTTACACATGCCTCGTAAGCAGAATGAATGTGCTACTGGAGGTCCGATTACGCTTTCTGCTGTTAGTAGAATACATCATGGAAGTAATGGCTGGCGAGGCACTGTTAGTAGTGCTGCAGCTGCTGCTACGGGTAGAATCGGTTCTGTTACGGGAGCTATCGCTTCAGCTTTCCACAACTGCAAGGGAAATGCTTTGCATATGGACCATGGATCGTCCGAGGCTAGGTACCACATATTAGTTTTTACTCCAACTGGAAGTATGATACAGTACGTGTTGCAAGTGGGTTTAGATTCAGCCATAGCTTTACCGATATCCAGCACAGCTCTCGACTTAGTTCCTGAGTTCGATGCAAGAGTAGCAGTTGAAGCAGTCCAGAAATGGAATATTTCTCAGAAACAAAACCGTAGGGCCCGAGATAATAACGTTGACATATACGGTGACAATGGAGCTTTAGATTGCAATAAAAATTACTGTGGAGAAATGAATGGAAAGCCTTCTGTTCTTGAAGCTAGTGGCACTGTCTCGAAGGCAAAGACTTGCACGGAGGAACAACACCACTTGTATATCTCGGAAGCGGAACTGCAGATGCATGCTGACCGTACTCCGCTGTGGACAAAACCCGAGGTACTTGCTGTTTGACTCGTATCTCTTCCAGATATATGTCTCATTTGTTTGATAATGACATGCCTGCATCCCTTTTTTATTGATGAACAGATATATTTTCAAGTGATGGCAAGAGATGGTGTCAAAGGTGACGAAATAGATCAACTAGGAGAAACCGAAATCGAAAGGATCCCGACTCGTATGGTTGAAGCTCGGTCGAAGGACCTAGTTCCAGTTTTTAACCATCTTCAGTCACCTAAAATCTCACAGACAAGGTGAGGTAAATTTTAGATGTCGATGAACGTAATCTTTTATGCTTTGATGATGAGCTTAACGTCGTCGTCGTAAATTATATATGAACAGAACGCCTGCTATCACTCACACACAACTCCTGATTTTTGCTTCTCATCATATTAGTCCTCTGAAATTCAGGTCTTTAAATAGGAACAGTGACCAACTATTGCTGCACCAGAAGTCCGACCTATTTGAAAATGGAAGGCAATCAAGGAGGAACGATGCGAGTCCCAATGGGTCGGTTATCAGCAACGGACCTAAAGGAACGGGTAAGCATGGTTACAAAATGGTGACAGAAACCAAGGGCGTTGTAAATGGCTATGAAGGCCCAAGGTCGTTGCATCCCGAGCTCGATAATGTAAATAATTGTACAGAGAGCTTAAGCATGGAGTGTTGTCAACCCAAGTCTGTAAATAACCATGAAAGTGGCGTGGAGGTGGAGGATGATTTTGAAGGCATATGTGATTTGTTTGAT

mRNA sequence

TTTTTTGTCTTCCTTTTATCTATTCCTTTTGTGGGTATTGAGATTTTGTTTACTGGGTTTGCACCCTTTTCGTTGCTTGGTGCAAATGACTCATGATCAGAATTGAGGCGATTTAGTTTCATTGATTTGGGAATTCTCTTCTGTTGATTATGTGAGTTCTTAAAAGGGTCTGATGATTGCTGATGATTGAAGCAGATGGAGAGTTGGAAATTGAATGTGTAATTGCTGGGGATTGACAAGAAACAGGGTGGGGTTATGATTAGAGTGGAATTTGTAGATTATTTTGCACTTCGATTCTGGGGATGGGGAATGATAGAGCTGTAAAACAACAGGGAACCGTAGCGCGCTCTGGGCGTGCTCATGAGTTCATATCAAGTTCGTTTCGTGCTTTTTCTAGTTACATGAAGATTGTTTCTGCTGGTGCTTCGACTGTGGCACGGTCCGCTGCATCAGTGGCATCTTCTTTGGTGGATAAGGAGGATGAAGCAAATGGCTTCCAGGTGAACTGGGCTGGTTTTGACAAGTTAGAGTGGGACGACAATGTCGTTCGACGGGTTCTTCTGTTAGGTTATCGTTCGGGTTTCCAGGTTTGGGACGTTGAGGAAGCAAATAATGTCCAAGACCTGGTTTCTCGTTACGATGGTTCTGTTTCATATATGCAAGTGTTGCCCAGACCAATACCGTCGATGAGATCGGGTGATAAGTTTACAGAAAGTAGACCATTGCTGGTGCTTTCTGCTTATGGTTCCATTACTGCTGGTTTTAAAATTCAGGATCGATTAGCAACTTCTGGCAATGCAGCCGTCCCAAAGGTCCAGGAACTAGCCGTTGCCGGTTTTATGCCTACTTTTGTTAGGTTTTATTCCTTGAAATCTCAAACCTACGTGCACGAGCTCAAATTTAGATCGGTTGTTTATTCTGTGAGATGCAGTGCCCTGGTTGTTGCCATTTCTCTCTTTACTCAGATACACTGCATCAATGCTACAACTTTGGAGACAGAACATATCATTCATACAAATCCTGTTGCTTCTGGATTTCTGGGTTCTGGAGGAGGTATCGGTTATGGACCTCTTGCGCTGGGATCCCGGTGGCTGGCTTATAGTGGAAGTCCTATCATTGCGTCGAATGTAGGGCGAGTGACACCACAGCACTTAAAACCTTCTGCTAGTTTATCTCATTCTTCTTTAAATGGAAGTTTAGTTGCTCACTATGCGAAGGAATCGAGTAAGCAACTTGCAGCAGGCCTTGTTACCATCGGTGATAAGGGTATTAAGAAGCTATCGAGATATTACTCGGAGCTCTTGCCCGATAGCAACAATTCTCATCAACATGGAGCTCAGGGTTTGAATGGTATTGGATATCTTAAAGATCATGTGGCTGATGCAGATAGTGTTGGAATGGTTATTGTGAAAGACATTATCAGCAAATCGGTTATCACGCAGTTTCGGGCTCATAAGAGCCCTATTTCTGCTTTATGCTTTGATCCGAGCGGCACCATCTTGGTGACAGCTTCTATTCAGGGTCATAACATAAATGTTTTCAACATTACGCCGAGTTCGAGTTCGAGTTCGAATTCATCTGTGTCTAGTACTGGTGCGTCTTATCGCCATCTCTACAGACTCCAACGAGGCTTCACTAATGCAGTCATACAAGACATCAGTTTCAGCTATGATAGCAACTGGATCATGATTAGTTCGTCCAGAGGGACGAGTCATCTCTTTGCTATAAACCCTGCTGGAGGACAGGTGAATTTCCCATCTACTAGTTCTGCTACCAGAACTGATGGGTTAGTAGTTCCTGCAAGACAAACCGTTTGGTTGGTCGATTCTGGTTTACACATGCCTCGTAAGCAGAATGAATGTGCTACTGGAGGTCCGATTACGCTTTCTGCTGTTAGTAGAATACATCATGGAAGTAATGGCTGGCGAGGCACTGTTAGTAGTGCTGCAGCTGCTGCTACGGGTAGAATCGGTTCTGTTACGGGAGCTATCGCTTCAGCTTTCCACAACTGCAAGGGAAATGCTTTGCATATGGACCATGGATCGTCCGAGGCTAGGTACCACATATTAGTTTTTACTCCAACTGGAAGTATGATACAGTACGTGTTGCAAGTGGGTTTAGATTCAGCCATAGCTTTACCGATATCCAGCACAGCTCTCGACTTAGTTCCTGAGTTCGATGCAAGAGTAGCAGTTGAAGCAGTCCAGAAATGGAATATTTCTCAGAAACAAAACCGTAGGGCCCGAGATAATAACGTTGACATATACGGTGACAATGGAGCTTTAGATTGCAATAAAAATTACTGTGGAGAAATGAATGGAAAGCCTTCTGTTCTTGAAGCTAGTGGCACTGTCTCGAAGGCAAAGACTTGCACGGAGGAACAACACCACTTGTATATCTCGGAAGCGGAACTGCAGATGCATGCTGACCGTACTCCGCTGTGGACAAAACCCGAGATATATTTTCAAGTGATGGCAAGAGATGGTGTCAAAGGTGACGAAATAGATCAACTAGGAGAAACCGAAATCGAAAGGATCCCGACTCGTATGGTTGAAGCTCGGTCGAAGGACCTAGTTCCAGTTTTTAACCATCTTCAGTCACCTAAAATCTCACAGACAAGGTCTTTAAATAGGAACAGTGACCAACTATTGCTGCACCAGAAGTCCGACCTATTTGAAAATGGAAGGCAATCAAGGAGGAACGATGCGAGTCCCAATGGGTCGGTTATCAGCAACGGACCTAAAGGAACGGGTAAGCATGGTTACAAAATGGTGACAGAAACCAAGGGCGTTGTAAATGGCTATGAAGGCCCAAGGTCGTTGCATCCCGAGCTCGATAATGTAAATAATTGTACAGAGAGCTTAAGCATGGAGTGTTGTCAACCCAAGTCTGTAAATAACCATGAAAGTGGCGTGGAGGTGGAGGATGATTTTGAAGGCATATGTGATTTGTTTGAT

Coding sequence (CDS)

ATGGGGAATGATAGAGCTGTAAAACAACAGGGAACCGTAGCGCGCTCTGGGCGTGCTCATGAGTTCATATCAAGTTCGTTTCGTGCTTTTTCTAGTTACATGAAGATTGTTTCTGCTGGTGCTTCGACTGTGGCACGGTCCGCTGCATCAGTGGCATCTTCTTTGGTGGATAAGGAGGATGAAGCAAATGGCTTCCAGGTGAACTGGGCTGGTTTTGACAAGTTAGAGTGGGACGACAATGTCGTTCGACGGGTTCTTCTGTTAGGTTATCGTTCGGGTTTCCAGGTTTGGGACGTTGAGGAAGCAAATAATGTCCAAGACCTGGTTTCTCGTTACGATGGTTCTGTTTCATATATGCAAGTGTTGCCCAGACCAATACCGTCGATGAGATCGGGTGATAAGTTTACAGAAAGTAGACCATTGCTGGTGCTTTCTGCTTATGGTTCCATTACTGCTGGTTTTAAAATTCAGGATCGATTAGCAACTTCTGGCAATGCAGCCGTCCCAAAGGTCCAGGAACTAGCCGTTGCCGGTTTTATGCCTACTTTTGTTAGGTTTTATTCCTTGAAATCTCAAACCTACGTGCACGAGCTCAAATTTAGATCGGTTGTTTATTCTGTGAGATGCAGTGCCCTGGTTGTTGCCATTTCTCTCTTTACTCAGATACACTGCATCAATGCTACAACTTTGGAGACAGAACATATCATTCATACAAATCCTGTTGCTTCTGGATTTCTGGGTTCTGGAGGAGGTATCGGTTATGGACCTCTTGCGCTGGGATCCCGGTGGCTGGCTTATAGTGGAAGTCCTATCATTGCGTCGAATGTAGGGCGAGTGACACCACAGCACTTAAAACCTTCTGCTAGTTTATCTCATTCTTCTTTAAATGGAAGTTTAGTTGCTCACTATGCGAAGGAATCGAGTAAGCAACTTGCAGCAGGCCTTGTTACCATCGGTGATAAGGGTATTAAGAAGCTATCGAGATATTACTCGGAGCTCTTGCCCGATAGCAACAATTCTCATCAACATGGAGCTCAGGGTTTGAATGGTATTGGATATCTTAAAGATCATGTGGCTGATGCAGATAGTGTTGGAATGGTTATTGTGAAAGACATTATCAGCAAATCGGTTATCACGCAGTTTCGGGCTCATAAGAGCCCTATTTCTGCTTTATGCTTTGATCCGAGCGGCACCATCTTGGTGACAGCTTCTATTCAGGGTCATAACATAAATGTTTTCAACATTACGCCGAGTTCGAGTTCGAGTTCGAATTCATCTGTGTCTAGTACTGGTGCGTCTTATCGCCATCTCTACAGACTCCAACGAGGCTTCACTAATGCAGTCATACAAGACATCAGTTTCAGCTATGATAGCAACTGGATCATGATTAGTTCGTCCAGAGGGACGAGTCATCTCTTTGCTATAAACCCTGCTGGAGGACAGGTGAATTTCCCATCTACTAGTTCTGCTACCAGAACTGATGGGTTAGTAGTTCCTGCAAGACAAACCGTTTGGTTGGTCGATTCTGGTTTACACATGCCTCGTAAGCAGAATGAATGTGCTACTGGAGGTCCGATTACGCTTTCTGCTGTTAGTAGAATACATCATGGAAGTAATGGCTGGCGAGGCACTGTTAGTAGTGCTGCAGCTGCTGCTACGGGTAGAATCGGTTCTGTTACGGGAGCTATCGCTTCAGCTTTCCACAACTGCAAGGGAAATGCTTTGCATATGGACCATGGATCGTCCGAGGCTAGGTACCACATATTAGTTTTTACTCCAACTGGAAGTATGATACAGTACGTGTTGCAAGTGGGTTTAGATTCAGCCATAGCTTTACCGATATCCAGCACAGCTCTCGACTTAGTTCCTGAGTTCGATGCAAGAGTAGCAGTTGAAGCAGTCCAGAAATGGAATATTTCTCAGAAACAAAACCGTAGGGCCCGAGATAATAACGTTGACATATACGGTGACAATGGAGCTTTAGATTGCAATAAAAATTACTGTGGAGAAATGAATGGAAAGCCTTCTGTTCTTGAAGCTAGTGGCACTGTCTCGAAGGCAAAGACTTGCACGGAGGAACAACACCACTTGTATATCTCGGAAGCGGAACTGCAGATGCATGCTGACCGTACTCCGCTGTGGACAAAACCCGAGATATATTTTCAAGTGATGGCAAGAGATGGTGTCAAAGGTGACGAAATAGATCAACTAGGAGAAACCGAAATCGAAAGGATCCCGACTCGTATGGTTGAAGCTCGGTCGAAGGACCTAGTTCCAGTTTTTAACCATCTTCAGTCACCTAAAATCTCACAGACAAGGTCTTTAAATAGGAACAGTGACCAACTATTGCTGCACCAGAAGTCCGACCTATTTGAAAATGGAAGGCAATCAAGGAGGAACGATGCGAGTCCCAATGGGTCGGTTATCAGCAACGGACCTAAAGGAACGGGTAAGCATGGTTACAAAATGGTGACAGAAACCAAGGGCGTTGTAAATGGCTATGAAGGCCCAAGGTCGTTGCATCCCGAGCTCGATAATGTAAATAATTGTACAGAGAGCTTAAGCATGGAGTGTTGTCAACCCAAGTCTGTAAATAACCATGAAAGTGGCGTGGAGGTGGAGGATGATTTTGAAGGCATATGTGATTTGTTTGAT

Protein sequence

MGNDRAVKQQGTVARSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSLVDKEDEANGFQVNWAGFDKLEWDDNVVRRVLLLGYRSGFQVWDVEEANNVQDLVSRYDGSVSYMQVLPRPIPSMRSGDKFTESRPLLVLSAYGSITAGFKIQDRLATSGNAAVPKVQELAVAGFMPTFVRFYSLKSQTYVHELKFRSVVYSVRCSALVVAISLFTQIHCINATTLETEHIIHTNPVASGFLGSGGGIGYGPLALGSRWLAYSGSPIIASNVGRVTPQHLKPSASLSHSSLNGSLVAHYAKESSKQLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQHGAQGLNGIGYLKDHVADADSVGMVIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITPSSSSSSNSSVSSTGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGGQVNFPSTSSATRTDGLVVPARQTVWLVDSGLHMPRKQNECATGGPITLSAVSRIHHGSNGWRGTVSSAAAAATGRIGSVTGAIASAFHNCKGNALHMDHGSSEARYHILVFTPTGSMIQYVLQVGLDSAIALPISSTALDLVPEFDARVAVEAVQKWNISQKQNRRARDNNVDIYGDNGALDCNKNYCGEMNGKPSVLEASGTVSKAKTCTEEQHHLYISEAELQMHADRTPLWTKPEIYFQVMARDGVKGDEIDQLGETEIERIPTRMVEARSKDLVPVFNHLQSPKISQTRSLNRNSDQLLLHQKSDLFENGRQSRRNDASPNGSVISNGPKGTGKHGYKMVTETKGVVNGYEGPRSLHPELDNVNNCTESLSMECCQPKSVNNHESGVEVEDDFEGICDLFD
Homology
BLAST of Carg05648 vs. NCBI nr
Match: KAG7015218.1 (Autophagy-related protein 18f, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1734.5 bits (4491), Expect = 0.0e+00
Identity = 885/885 (100.00%), Postives = 885/885 (100.00%), Query Frame = 0

Query: 1   MGNDRAVKQQGTVARSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSLVDKED 60
           MGNDRAVKQQGTVARSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSLVDKED
Sbjct: 1   MGNDRAVKQQGTVARSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSLVDKED 60

Query: 61  EANGFQVNWAGFDKLEWDDNVVRRVLLLGYRSGFQVWDVEEANNVQDLVSRYDGSVSYMQ 120
           EANGFQVNWAGFDKLEWDDNVVRRVLLLGYRSGFQVWDVEEANNVQDLVSRYDGSVSYMQ
Sbjct: 61  EANGFQVNWAGFDKLEWDDNVVRRVLLLGYRSGFQVWDVEEANNVQDLVSRYDGSVSYMQ 120

Query: 121 VLPRPIPSMRSGDKFTESRPLLVLSAYGSITAGFKIQDRLATSGNAAVPKVQELAVAGFM 180
           VLPRPIPSMRSGDKFTESRPLLVLSAYGSITAGFKIQDRLATSGNAAVPKVQELAVAGFM
Sbjct: 121 VLPRPIPSMRSGDKFTESRPLLVLSAYGSITAGFKIQDRLATSGNAAVPKVQELAVAGFM 180

Query: 181 PTFVRFYSLKSQTYVHELKFRSVVYSVRCSALVVAISLFTQIHCINATTLETEHIIHTNP 240
           PTFVRFYSLKSQTYVHELKFRSVVYSVRCSALVVAISLFTQIHCINATTLETEHIIHTNP
Sbjct: 181 PTFVRFYSLKSQTYVHELKFRSVVYSVRCSALVVAISLFTQIHCINATTLETEHIIHTNP 240

Query: 241 VASGFLGSGGGIGYGPLALGSRWLAYSGSPIIASNVGRVTPQHLKPSASLSHSSLNGSLV 300
           VASGFLGSGGGIGYGPLALGSRWLAYSGSPIIASNVGRVTPQHLKPSASLSHSSLNGSLV
Sbjct: 241 VASGFLGSGGGIGYGPLALGSRWLAYSGSPIIASNVGRVTPQHLKPSASLSHSSLNGSLV 300

Query: 301 AHYAKESSKQLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQHGAQGLNGIGYLKDHVAD 360
           AHYAKESSKQLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQHGAQGLNGIGYLKDHVAD
Sbjct: 301 AHYAKESSKQLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQHGAQGLNGIGYLKDHVAD 360

Query: 361 ADSVGMVIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITPSSS 420
           ADSVGMVIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITPSSS
Sbjct: 361 ADSVGMVIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITPSSS 420

Query: 421 SSSNSSVSSTGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGG 480
           SSSNSSVSSTGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGG
Sbjct: 421 SSSNSSVSSTGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGG 480

Query: 481 QVNFPSTSSATRTDGLVVPARQTVWLVDSGLHMPRKQNECATGGPITLSAVSRIHHGSNG 540
           QVNFPSTSSATRTDGLVVPARQTVWLVDSGLHMPRKQNECATGGPITLSAVSRIHHGSNG
Sbjct: 481 QVNFPSTSSATRTDGLVVPARQTVWLVDSGLHMPRKQNECATGGPITLSAVSRIHHGSNG 540

Query: 541 WRGTVSSAAAAATGRIGSVTGAIASAFHNCKGNALHMDHGSSEARYHILVFTPTGSMIQY 600
           WRGTVSSAAAAATGRIGSVTGAIASAFHNCKGNALHMDHGSSEARYHILVFTPTGSMIQY
Sbjct: 541 WRGTVSSAAAAATGRIGSVTGAIASAFHNCKGNALHMDHGSSEARYHILVFTPTGSMIQY 600

Query: 601 VLQVGLDSAIALPISSTALDLVPEFDARVAVEAVQKWNISQKQNRRARDNNVDIYGDNGA 660
           VLQVGLDSAIALPISSTALDLVPEFDARVAVEAVQKWNISQKQNRRARDNNVDIYGDNGA
Sbjct: 601 VLQVGLDSAIALPISSTALDLVPEFDARVAVEAVQKWNISQKQNRRARDNNVDIYGDNGA 660

Query: 661 LDCNKNYCGEMNGKPSVLEASGTVSKAKTCTEEQHHLYISEAELQMHADRTPLWTKPEIY 720
           LDCNKNYCGEMNGKPSVLEASGTVSKAKTCTEEQHHLYISEAELQMHADRTPLWTKPEIY
Sbjct: 661 LDCNKNYCGEMNGKPSVLEASGTVSKAKTCTEEQHHLYISEAELQMHADRTPLWTKPEIY 720

Query: 721 FQVMARDGVKGDEIDQLGETEIERIPTRMVEARSKDLVPVFNHLQSPKISQTRSLNRNSD 780
           FQVMARDGVKGDEIDQLGETEIERIPTRMVEARSKDLVPVFNHLQSPKISQTRSLNRNSD
Sbjct: 721 FQVMARDGVKGDEIDQLGETEIERIPTRMVEARSKDLVPVFNHLQSPKISQTRSLNRNSD 780

Query: 781 QLLLHQKSDLFENGRQSRRNDASPNGSVISNGPKGTGKHGYKMVTETKGVVNGYEGPRSL 840
           QLLLHQKSDLFENGRQSRRNDASPNGSVISNGPKGTGKHGYKMVTETKGVVNGYEGPRSL
Sbjct: 781 QLLLHQKSDLFENGRQSRRNDASPNGSVISNGPKGTGKHGYKMVTETKGVVNGYEGPRSL 840

Query: 841 HPELDNVNNCTESLSMECCQPKSVNNHESGVEVEDDFEGICDLFD 886
           HPELDNVNNCTESLSMECCQPKSVNNHESGVEVEDDFEGICDLFD
Sbjct: 841 HPELDNVNNCTESLSMECCQPKSVNNHESGVEVEDDFEGICDLFD 885

BLAST of Carg05648 vs. NCBI nr
Match: KAG6607820.1 (Autophagy-related protein 18f, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1717.6 bits (4447), Expect = 0.0e+00
Identity = 878/885 (99.21%), Postives = 881/885 (99.55%), Query Frame = 0

Query: 1   MGNDRAVKQQGTVARSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSLVDKED 60
           MGNDRAVKQQGTVARSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSLVDKED
Sbjct: 1   MGNDRAVKQQGTVARSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSLVDKED 60

Query: 61  EANGFQVNWAGFDKLEWDDNVVRRVLLLGYRSGFQVWDVEEANNVQDLVSRYDGSVSYMQ 120
           EANGFQVNWAGFDKLEWDDNVVRRVLLLGYRSGFQVWDVEEANNVQDLVSRYDGSVSYMQ
Sbjct: 61  EANGFQVNWAGFDKLEWDDNVVRRVLLLGYRSGFQVWDVEEANNVQDLVSRYDGSVSYMQ 120

Query: 121 VLPRPIPSMRSGDKFTESRPLLVLSAYGSITAGFKIQDRLATSGNAAVPKVQELAVAGFM 180
           VLPRPIPSMRSGDKFTESRPLLVLSAYGSITAGFKIQDRLATSGNAAVPKVQELAVAGFM
Sbjct: 121 VLPRPIPSMRSGDKFTESRPLLVLSAYGSITAGFKIQDRLATSGNAAVPKVQELAVAGFM 180

Query: 181 PTFVRFYSLKSQTYVHELKFRSVVYSVRCSALVVAISLFTQIHCINATTLETEHIIHTNP 240
           PTFVRFYSLKSQTYVHELKFRSVVYSVRCS LVVAISLFTQIHCINATTLETEHIIHTNP
Sbjct: 181 PTFVRFYSLKSQTYVHELKFRSVVYSVRCSPLVVAISLFTQIHCINATTLETEHIIHTNP 240

Query: 241 VASGFLGSGGGIGYGPLALGSRWLAYSGSPIIASNVGRVTPQHLKPSASLSHSSLNGSLV 300
           VASGFLGSGGGIGYGPLALGSRWLAYSGSPIIASNVGRVTPQHLKPSASLSHSSLNGSLV
Sbjct: 241 VASGFLGSGGGIGYGPLALGSRWLAYSGSPIIASNVGRVTPQHLKPSASLSHSSLNGSLV 300

Query: 301 AHYAKESSKQLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQHGAQGLNGIGYLKDHVAD 360
           AHYAKESSKQLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQHGAQGLNGIGYLKDHVAD
Sbjct: 301 AHYAKESSKQLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQHGAQGLNGIGYLKDHVAD 360

Query: 361 ADSVGMVIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITPSSS 420
           ADSVGMVIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITPSSS
Sbjct: 361 ADSVGMVIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITPSSS 420

Query: 421 SSSNSSVSSTGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGG 480
           SSSNSSVSSTGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAIN AGG
Sbjct: 421 SSSNSSVSSTGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINSAGG 480

Query: 481 QVNFPSTSSATRTDGLVVPARQTVWLVDSGLHMPRKQNECATGGPITLSAVSRIHHGSNG 540
           QVNFPSTSSATRTDGLVVPARQTV LVDSGLH+P KQNECATGGPITLSAVSRIHHGSNG
Sbjct: 481 QVNFPSTSSATRTDGLVVPARQTVRLVDSGLHIPCKQNECATGGPITLSAVSRIHHGSNG 540

Query: 541 WRGTVSSAAAAATGRIGSVTGAIASAFHNCKGNALHMDHGSSEARYHILVFTPTGSMIQY 600
           WRGTVSSAAAAATGRIGSVTGAIASAFHNCKGNALHMDHGSSEARYHILVFTPTGSMIQY
Sbjct: 541 WRGTVSSAAAAATGRIGSVTGAIASAFHNCKGNALHMDHGSSEARYHILVFTPTGSMIQY 600

Query: 601 VLQVGLDSAIALPISSTALDLVPEFDARVAVEAVQKWNISQKQNRRARDNNVDIYGDNGA 660
           VL+VGLDSA+ALPISSTALDLVPEFDARVAVEAVQKWNISQKQNRRARDNNVDIYGDNGA
Sbjct: 601 VLRVGLDSAVALPISSTALDLVPEFDARVAVEAVQKWNISQKQNRRARDNNVDIYGDNGA 660

Query: 661 LDCNKNYCGEMNGKPSVLEASGTVSKAKTCTEEQHHLYISEAELQMHADRTPLWTKPEIY 720
           LDCNKNYCGEMNGKPSVLEASGTVSKAKTCTEEQHHLYISEAELQMHADRTPLWTKPEIY
Sbjct: 661 LDCNKNYCGEMNGKPSVLEASGTVSKAKTCTEEQHHLYISEAELQMHADRTPLWTKPEIY 720

Query: 721 FQVMARDGVKGDEIDQLGETEIERIPTRMVEARSKDLVPVFNHLQSPKISQTRSLNRNSD 780
           FQVMARDGVKGDEIDQLGETEIERIPTRMVEARSKDLVPVFNHLQSPKISQTRSLNRNSD
Sbjct: 721 FQVMARDGVKGDEIDQLGETEIERIPTRMVEARSKDLVPVFNHLQSPKISQTRSLNRNSD 780

Query: 781 QLLLHQKSDLFENGRQSRRNDASPNGSVISNGPKGTGKHGYKMVTETKGVVNGYEGPRSL 840
           QLLLHQKSDLFENGRQSRRNDASPNGSVISNGPKGTGKHGYKMVTETKGVVNGYEGPRSL
Sbjct: 781 QLLLHQKSDLFENGRQSRRNDASPNGSVISNGPKGTGKHGYKMVTETKGVVNGYEGPRSL 840

Query: 841 HPELDNVNNCTESLSMECCQPKSVNNHESGVEVEDDFEGICDLFD 886
           HPELDNVNNCTESLSMECCQPKSVNNHESGVEVEDDFEGICDLFD
Sbjct: 841 HPELDNVNNCTESLSMECCQPKSVNNHESGVEVEDDFEGICDLFD 885

BLAST of Carg05648 vs. NCBI nr
Match: XP_022940185.1 (autophagy-related protein 18f-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1687.2 bits (4368), Expect = 0.0e+00
Identity = 867/885 (97.97%), Postives = 871/885 (98.42%), Query Frame = 0

Query: 1   MGNDRAVKQQGTVARSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSLVDKED 60
           MGNDRAVKQQGTVARSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSLVDK+D
Sbjct: 1   MGNDRAVKQQGTVARSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSLVDKDD 60

Query: 61  EANGFQVNWAGFDKLEWDDNVVRRVLLLGYRSGFQVWDVEEANNVQDLVSRYDGSVSYMQ 120
           EANGFQVNWAGFDKLEWDDNVVRRVLLLGY SGFQVWDVEEANNVQDLVSRYDGSVSYMQ
Sbjct: 61  EANGFQVNWAGFDKLEWDDNVVRRVLLLGYCSGFQVWDVEEANNVQDLVSRYDGSVSYMQ 120

Query: 121 VLPRPIPSMRSGDKFTESRPLLVLSAYGSITAGFKIQDRLATSGNAAVPKVQELAVAGFM 180
           VLPRPIPSMRSGDKFTESRPLLVLSAYGSITAGFKIQDR+ATSGNAAVPKVQE A AGFM
Sbjct: 121 VLPRPIPSMRSGDKFTESRPLLVLSAYGSITAGFKIQDRIATSGNAAVPKVQEPADAGFM 180

Query: 181 PTFVRFYSLKSQTYVHELKFRSVVYSVRCSALVVAISLFTQIHCINATTLETEHIIHTNP 240
           PTFVRFYSLKSQTYVHELKFRSVVYSVRCS LVVAISL TQIHCINATTLETEHIIHTNP
Sbjct: 181 PTFVRFYSLKSQTYVHELKFRSVVYSVRCSPLVVAISLVTQIHCINATTLETEHIIHTNP 240

Query: 241 VASGFLGSGGGIGYGPLALGSRWLAYSGSPIIASNVGRVTPQHLKPSASLSHSSLNGSLV 300
           VASGFL SGGGIGYGPLALGSRWLAYSGSPI ASNVGRVTPQHLKPSASLSHSSLNGSLV
Sbjct: 241 VASGFLCSGGGIGYGPLALGSRWLAYSGSPITASNVGRVTPQHLKPSASLSHSSLNGSLV 300

Query: 301 AHYAKESSKQLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQHGAQGLNGIGYLKDHVAD 360
           AHYAKESSKQLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQHGAQGLNGIGYLKDHVAD
Sbjct: 301 AHYAKESSKQLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQHGAQGLNGIGYLKDHVAD 360

Query: 361 ADSVGMVIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITPSSS 420
           ADSVGMVIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITP  S
Sbjct: 361 ADSVGMVIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITP--S 420

Query: 421 SSSNSSVSSTGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGG 480
           SSSNSSVSSTGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGG
Sbjct: 421 SSSNSSVSSTGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGG 480

Query: 481 QVNFPSTSSATRTDGLVVPARQTVWLVDSGLHMPRKQNECATGGPITLSAVSRIHHGSNG 540
           QVNFPSTSSATRTDGLVVPARQTV LVDSGLHMPRKQNECAT GPITLSAVSRIHHGSNG
Sbjct: 481 QVNFPSTSSATRTDGLVVPARQTVRLVDSGLHMPRKQNECATRGPITLSAVSRIHHGSNG 540

Query: 541 WRGTVSSAAAAATGRIGSVTGAIASAFHNCKGNALHMDHGSSEARYHILVFTPTGSMIQY 600
           WRGTVSSAAAAATGRIGSVTGAIASAFHNCKGNALHMDHGSSEARYHILVFTPTGSMIQY
Sbjct: 541 WRGTVSSAAAAATGRIGSVTGAIASAFHNCKGNALHMDHGSSEARYHILVFTPTGSMIQY 600

Query: 601 VLQVGLDSAIALPISSTALDLVPEFDARVAVEAVQKWNISQKQNRRARDNNVDIYGDNGA 660
           VL+VGLDSAIALPISSTALDLVPEFDARVAVEAVQKWNISQKQNRRARDNNVDIYGDNGA
Sbjct: 601 VLRVGLDSAIALPISSTALDLVPEFDARVAVEAVQKWNISQKQNRRARDNNVDIYGDNGA 660

Query: 661 LDCNKNYCGEMNGKPSVLEASGTVSKAKTCTEEQHHLYISEAELQMHADRTPLWTKPEIY 720
           LDCNKNYC EMNGKPSVLEASGTVSKAKTCTEEQHHLYISEAELQMHADRTPLWTKPEIY
Sbjct: 661 LDCNKNYCVEMNGKPSVLEASGTVSKAKTCTEEQHHLYISEAELQMHADRTPLWTKPEIY 720

Query: 721 FQVMARDGVKGDEIDQLGETEIERIPTRMVEARSKDLVPVFNHLQSPKISQTRSLNRNSD 780
           FQVMARDGVKGDEIDQLGETEIERIPTRMVEARSKDLVPVFNHLQ PKISQTRSLNRNSD
Sbjct: 721 FQVMARDGVKGDEIDQLGETEIERIPTRMVEARSKDLVPVFNHLQLPKISQTRSLNRNSD 780

Query: 781 QLLLHQKSDLFENGRQSRRNDASPNGSVISNGPKGTGKHGYKMVTETKGVVNGYEGPRSL 840
           QLLLHQKSDLFENGRQSR NDASPNGSVISNGPKGTGKHGYK+VTETKGVVNGYEGPRSL
Sbjct: 781 QLLLHQKSDLFENGRQSRGNDASPNGSVISNGPKGTGKHGYKLVTETKGVVNGYEGPRSL 840

Query: 841 HPELDNVNNCTESLSMECCQPKSVNNHESGVEVEDDFEGICDLFD 886
           HPELDNVNNCTESLSMECCQPKSVNNHESGVEVEDDFEGICDLFD
Sbjct: 841 HPELDNVNNCTESLSMECCQPKSVNNHESGVEVEDDFEGICDLFD 883

BLAST of Carg05648 vs. NCBI nr
Match: XP_022940186.1 (autophagy-related protein 18f-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1674.4 bits (4335), Expect = 0.0e+00
Identity = 863/885 (97.51%), Postives = 867/885 (97.97%), Query Frame = 0

Query: 1   MGNDRAVKQQGTVARSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSLVDKED 60
           MGNDRAVKQQGTVARSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSLVDK+D
Sbjct: 1   MGNDRAVKQQGTVARSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSLVDKDD 60

Query: 61  EANGFQVNWAGFDKLEWDDNVVRRVLLLGYRSGFQVWDVEEANNVQDLVSRYDGSVSYMQ 120
           EANGFQVNWAGFDKLEWDDNVVRRVLLLGY SGFQVWDVEEANNVQDLVSRYDGSVSYMQ
Sbjct: 61  EANGFQVNWAGFDKLEWDDNVVRRVLLLGYCSGFQVWDVEEANNVQDLVSRYDGSVSYMQ 120

Query: 121 VLPRPIPSMRSGDKFTESRPLLVLSAYGSITAGFKIQDRLATSGNAAVPKVQELAVAGFM 180
           VLPRPIPSMRSGDKFTESRPLLVLSAYGSITAGFKIQDR+ATSGNAAVPKVQE A AGFM
Sbjct: 121 VLPRPIPSMRSGDKFTESRPLLVLSAYGSITAGFKIQDRIATSGNAAVPKVQEPADAGFM 180

Query: 181 PTFVRFYSLKSQTYVHELKFRSVVYSVRCSALVVAISLFTQIHCINATTLETEHIIHTNP 240
           PTFVRFYSLKSQTYVHELKFRSVVYSVRCS LVVAISL TQIHCINATTLETEHIIHTNP
Sbjct: 181 PTFVRFYSLKSQTYVHELKFRSVVYSVRCSPLVVAISLVTQIHCINATTLETEHIIHTNP 240

Query: 241 VASGFLGSGGGIGYGPLALGSRWLAYSGSPIIASNVGRVTPQHLKPSASLSHSSLNGSLV 300
           VASGFL SGGGIGYGPLALGSRWLAYSGSPI ASNVGRVTPQHLKPSASLSHSSLNGSLV
Sbjct: 241 VASGFLCSGGGIGYGPLALGSRWLAYSGSPITASNVGRVTPQHLKPSASLSHSSLNGSLV 300

Query: 301 AHYAKESSKQLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQHGAQGLNGIGYLKDHVAD 360
           AHYAKESSKQLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQHGAQGLNGIGYLKDHVAD
Sbjct: 301 AHYAKESSKQLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQHGAQGLNGIGYLKDHVAD 360

Query: 361 ADSVGMVIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITPSSS 420
           ADSVGMVIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITP  S
Sbjct: 361 ADSVGMVIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITP--S 420

Query: 421 SSSNSSVSSTGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGG 480
           SSSNSSVSSTGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGG
Sbjct: 421 SSSNSSVSSTGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGG 480

Query: 481 QVNFPSTSSATRTDGLVVPARQTVWLVDSGLHMPRKQNECATGGPITLSAVSRIHHGSNG 540
           QVNFPSTSSATRTDGLVVPARQTV LVDSGLHMPRKQNECAT GPITLSAVSRIHHGSNG
Sbjct: 481 QVNFPSTSSATRTDGLVVPARQTVRLVDSGLHMPRKQNECATRGPITLSAVSRIHHGSNG 540

Query: 541 WRGTVSSAAAAATGRIGSVTGAIASAFHNCKGNALHMDHGSSEARYHILVFTPTGSMIQY 600
           WRGTVSSAAAAATGRIGSVTGAIASAFHNCKGNALHMDHGSSEARYHILVFTPTGSMIQY
Sbjct: 541 WRGTVSSAAAAATGRIGSVTGAIASAFHNCKGNALHMDHGSSEARYHILVFTPTGSMIQY 600

Query: 601 VLQVGLDSAIALPISSTALDLVPEFDARVAVEAVQKWNISQKQNRRARDNNVDIYGDNGA 660
           VL+VGLDSAIALPISSTALDLVPEFDARVAVEAVQKWNISQKQNRRARDNNVDIYGDNGA
Sbjct: 601 VLRVGLDSAIALPISSTALDLVPEFDARVAVEAVQKWNISQKQNRRARDNNVDIYGDNGA 660

Query: 661 LDCNKNYCGEMNGKPSVLEASGTVSKAKTCTEEQHHLYISEAELQMHADRTPLWTKPEIY 720
           LDCNKNYC EMNGKPSVLEASGTVSKAKTCTEEQHHLYISEAELQMHADRTPLWTKPEIY
Sbjct: 661 LDCNKNYCVEMNGKPSVLEASGTVSKAKTCTEEQHHLYISEAELQMHADRTPLWTKPEIY 720

Query: 721 FQVMARDGVKGDEIDQLGETEIERIPTRMVEARSKDLVPVFNHLQSPKISQTRSLNRNSD 780
           FQVMARDGVKGDEIDQLGETEIERIPTRMVEARSKDLVPVFNHLQ PKISQT    RNSD
Sbjct: 721 FQVMARDGVKGDEIDQLGETEIERIPTRMVEARSKDLVPVFNHLQLPKISQT----RNSD 780

Query: 781 QLLLHQKSDLFENGRQSRRNDASPNGSVISNGPKGTGKHGYKMVTETKGVVNGYEGPRSL 840
           QLLLHQKSDLFENGRQSR NDASPNGSVISNGPKGTGKHGYK+VTETKGVVNGYEGPRSL
Sbjct: 781 QLLLHQKSDLFENGRQSRGNDASPNGSVISNGPKGTGKHGYKLVTETKGVVNGYEGPRSL 840

Query: 841 HPELDNVNNCTESLSMECCQPKSVNNHESGVEVEDDFEGICDLFD 886
           HPELDNVNNCTESLSMECCQPKSVNNHESGVEVEDDFEGICDLFD
Sbjct: 841 HPELDNVNNCTESLSMECCQPKSVNNHESGVEVEDDFEGICDLFD 879

BLAST of Carg05648 vs. NCBI nr
Match: XP_023524854.1 (autophagy-related protein 18f-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1662.1 bits (4303), Expect = 0.0e+00
Identity = 856/885 (96.72%), Postives = 862/885 (97.40%), Query Frame = 0

Query: 1   MGNDRAVKQQGTVARSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSLVDKED 60
           MGNDRAVKQQGTV RSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSLVDK+D
Sbjct: 1   MGNDRAVKQQGTVPRSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSLVDKDD 60

Query: 61  EANGFQVNWAGFDKLEWDDNVVRRVLLLGYRSGFQVWDVEEANNVQDLVSRYDGSVSYMQ 120
           EANGFQVNWAGFDKLEWDDNVVR VLLLGYRSGFQVWDVEEANNVQDLVSRYDGSVSYMQ
Sbjct: 61  EANGFQVNWAGFDKLEWDDNVVRSVLLLGYRSGFQVWDVEEANNVQDLVSRYDGSVSYMQ 120

Query: 121 VLPRPIPSMRSGDKFTESRPLLVLSAYGSITAGFKIQDRLATSGNAAVPKVQELAVAGFM 180
           VLPRPIPSMRSGDKFTESRPLLVLSAYGSITAGFKIQDR+ATSGNAAVPKVQELA AGFM
Sbjct: 121 VLPRPIPSMRSGDKFTESRPLLVLSAYGSITAGFKIQDRIATSGNAAVPKVQELADAGFM 180

Query: 181 PTFVRFYSLKSQTYVHELKFRSVVYSVRCSALVVAISLFTQIHCINATTLETEHIIHTNP 240
           PTFVRFYSLKSQTYVHELKFRSVVYSVRCS LVVAISL TQIHCINATTLETEHIIHTNP
Sbjct: 181 PTFVRFYSLKSQTYVHELKFRSVVYSVRCSPLVVAISLVTQIHCINATTLETEHIIHTNP 240

Query: 241 VASGFLGSGGGIGYGPLALGSRWLAYSGSPIIASNVGRVTPQHLKPSASLSHSSLNGSLV 300
           VASGFLGSGGGIGYGPLALG RWLAYSGSPI ASNVGRVTPQHLKPSASLSHSSLNGSLV
Sbjct: 241 VASGFLGSGGGIGYGPLALGPRWLAYSGSPITASNVGRVTPQHLKPSASLSHSSLNGSLV 300

Query: 301 AHYAKESSKQLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQHGAQGLNGIGYLKDHVAD 360
           AHYAKESSKQLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQ GA GLNGIGYLKDHVAD
Sbjct: 301 AHYAKESSKQLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQPGAHGLNGIGYLKDHVAD 360

Query: 361 ADSVGMVIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITPSSS 420
           ADSVGMVIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITP  S
Sbjct: 361 ADSVGMVIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITP--S 420

Query: 421 SSSNSSVSSTGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGG 480
           SSSNS VSSTGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGG
Sbjct: 421 SSSNSPVSSTGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGG 480

Query: 481 QVNFPSTSSATRTDGLVVPARQTVWLVDSGLHMPRKQNECATGGPITLSAVSRIHHGSNG 540
           QV  PSTSSA RTDGLVVPARQTV LVDSGLHMPRKQNECATGGP+TLSAVSRIHHGSNG
Sbjct: 481 QV--PSTSSAIRTDGLVVPARQTVRLVDSGLHMPRKQNECATGGPMTLSAVSRIHHGSNG 540

Query: 541 WRGTVSSAAAAATGRIGSVTGAIASAFHNCKGNALHMDHGSSEARYHILVFTPTGSMIQY 600
           WRGTVSSAAAAATGRIGSVTGAIASAFHNCKGNA  +DHGSSEARYHILVFTPTGSMIQY
Sbjct: 541 WRGTVSSAAAAATGRIGSVTGAIASAFHNCKGNAFPVDHGSSEARYHILVFTPTGSMIQY 600

Query: 601 VLQVGLDSAIALPISSTALDLVPEFDARVAVEAVQKWNISQKQNRRARDNNVDIYGDNGA 660
           VL+VGLDSAIALPISSTALDLVPEFDARVAVEAVQKWNISQKQNRRARDNNVDIYGDNGA
Sbjct: 601 VLRVGLDSAIALPISSTALDLVPEFDARVAVEAVQKWNISQKQNRRARDNNVDIYGDNGA 660

Query: 661 LDCNKNYCGEMNGKPSVLEASGTVSKAKTCTEEQHHLYISEAELQMHADRTPLWTKPEIY 720
           LDCNKNYCGEMNGKP VLEASGTVSKAKTCTEEQHHLYISEAELQMHADRTPLWTKPEIY
Sbjct: 661 LDCNKNYCGEMNGKPIVLEASGTVSKAKTCTEEQHHLYISEAELQMHADRTPLWTKPEIY 720

Query: 721 FQVMARDGVKGDEIDQLGETEIERIPTRMVEARSKDLVPVFNHLQSPKISQTRSLNRNSD 780
           FQVMARDGVKGDE DQLGETEIERIPTRMVEARSKDLVPVFNHLQSP ISQTRSLNRNSD
Sbjct: 721 FQVMARDGVKGDETDQLGETEIERIPTRMVEARSKDLVPVFNHLQSPIISQTRSLNRNSD 780

Query: 781 QLLLHQKSDLFENGRQSRRNDASPNGSVISNGPKGTGKHGYKMVTETKGVVNGYEGPRSL 840
           QLLLHQKSDLFENGRQSRRND SP+GSVISNGPKGTGKHGYKMVTETKGVVNGYEGPRS 
Sbjct: 781 QLLLHQKSDLFENGRQSRRNDVSPDGSVISNGPKGTGKHGYKMVTETKGVVNGYEGPRSS 840

Query: 841 HPELDNVNNCTESLSMECCQPKSVNNHESGVEVEDDFEGICDLFD 886
           HPELDNVNNCTESLSMECCQPKSVNNHESGVEVEDDFEGICDLFD
Sbjct: 841 HPELDNVNNCTESLSMECCQPKSVNNHESGVEVEDDFEGICDLFD 881

BLAST of Carg05648 vs. ExPASy Swiss-Prot
Match: Q9FH32 (Autophagy-related protein 18f OS=Arabidopsis thaliana OX=3702 GN=ATG18F PE=2 SV=1)

HSP 1 Score: 515.0 bits (1325), Expect = 1.7e-144
Identity = 351/777 (45.17%), Postives = 462/777 (59.46%), Query Frame = 0

Query: 11  GTVARSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSLVDKEDEANGFQVNWA 70
           G V+RS R      SSFRA S+ +K++S+GASTVARSA S ASS V+   +    QV WA
Sbjct: 15  GVVSRSAR------SSFRALSNCLKVISSGASTVARSAVSAASSAVESHHD----QVLWA 74

Query: 71  GFDKLEWDDNVVRRVLLLGYRSGFQVWDVEEANNVQDLVSRYDGSVSYMQVLPRPIPSMR 130
           GFD L+ +D   RRVLLL ++SGFQVWDVE+  NV  +VS +DG   +MQ+L  PI S  
Sbjct: 75  GFDNLQKEDGDTRRVLLLAFKSGFQVWDVEDTENVHVIVSAHDGQAFFMQMLLNPINSGV 134

Query: 131 SGDKFTESRPLL-VLSAYGSITAGFKIQDRLATSGNAAVPKVQELAVAGFMPTFVRFYSL 190
             D+F +SRPLL V   Y S         +  +S N   P  + +A     PT V  YSL
Sbjct: 135 LDDRFYKSRPLLAVCGDYSS---------KKISSDN---PGSETVAT----PTNVYVYSL 194

Query: 191 KSQTYVHELKFRSVVYSVRCSALVVAISLFTQIHCINATTLETEHIIHTNPVASGFLGSG 250
           KSQ+YVH LKFR+ +YSVRC + +VA+    QI C +A TLE ++ I TN +  G L   
Sbjct: 195 KSQSYVHTLKFRATIYSVRCCSRIVAVLQAAQIDCFDAATLEMDYRIVTNSIVCGSL--- 254

Query: 251 GGIGYGPLALGSRWLAYSGSPIIASNVGRVTPQHLKPSASLSHSSLNGSLVAHYAKESSK 310
            G+GYGPLA+G RW+AYSGS I  S+    T +      SLS SS +   VA +A++SSK
Sbjct: 255 -GVGYGPLAVGPRWIAYSGSRIATSSSAIFTSE----IVSLSTSSPS---VAQFARDSSK 314

Query: 311 QLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQHGAQGLNGIGYLKDHVADADSVGMVIV 370
           QLA+G+  +GDKG + L++Y SE+LP+          GL GIG   + VADA+S+GMVIV
Sbjct: 315 QLASGIANLGDKGYRSLTKYCSEVLPNPY------IPGLKGIGVGNEKVADAESIGMVIV 374

Query: 371 KDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITPSSSSSSNSSVSS 430
           KDI +KSVITQF+AHKSPISALCFD SG +LVTASIQGHNINVF I P+ S+S   +V +
Sbjct: 375 KDITNKSVITQFKAHKSPISALCFDQSGLLLVTASIQGHNINVFRIMPTISTS--RAVKT 434

Query: 431 TGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGGQVNFPSTSS 490
           T  ++ HL+RLQRGFTNAVIQDI FS DSN I++ SSRGTSHLF INP            
Sbjct: 435 T--TFAHLFRLQRGFTNAVIQDICFSKDSNLIVVGSSRGTSHLFEINP------------ 494

Query: 491 ATRTDGLVVPARQTVWLVDSGLHMPRKQNECATGGPITLSAVSRIHHG-SNGWRGTVSSA 550
                                        E     P+ +SA+SRI  G S+GW GTVS A
Sbjct: 495 -----------------------------EKEGDAPVPMSAISRIRSGNSSGWIGTVSGA 554

Query: 551 AAAATGRI-GSVTGAIASAFHNCKGNALHMDHGSSE---ARYHILVFTPTGSMIQYVL-- 610
           A+AA G + GSV G + S F  C   + +  +GS     ++ ++LVF P+G M QY L  
Sbjct: 555 ASAAAGMVAGSVPGTVTSTFCYCDEKSNNNYYGSVADMCSKTNLLVFAPSGCMTQYALRE 614

Query: 611 -QVGLDSAIALPISSTALDLVP--EFDARVAVEAVQKWNISQKQNRR-ARDNNVDIYGDN 670
            QVG+    A   + T  D     E + ++AV+ +++W++ Q Q+RR   D + DIYG  
Sbjct: 615 HQVGVGHETA---AMTGFDSESGLETEGKLAVDPIRRWSMIQNQSRRETHDPHSDIYGGG 674

Query: 671 GALDCNKNYCGEMNGKPSVLEASGTVSKAKTCTEEQHHLYISEAELQMH--ADRTPLWTK 730
            ++D       E+  K SV EA     K  T   ++ HLYI EAE Q      + PLW +
Sbjct: 675 TSVDSKSKVFPEVVRKQSVEEAWKVSKKGTTRVVDKRHLYIYEAEQQQTHLPTQLPLWAR 698

Query: 731 PEIYFQVMARDGVKGDEIDQLG--ETEIERIPTRMVEARSKDLVPVFNHLQSPKISQ 772
            +  FQ +  +  +G+EI   G  E EIE I TR +EAR++DLVPV+ +LQSP+  Q
Sbjct: 735 RKFRFQELVLN--RGEEISGGGGREMEIEGIQTRTIEARTRDLVPVWGYLQSPRSQQ 698

BLAST of Carg05648 vs. ExPASy Swiss-Prot
Match: Q8GUL1 (Autophagy-related protein 18g OS=Arabidopsis thaliana OX=3702 GN=ATG18G PE=2 SV=1)

HSP 1 Score: 447.6 bits (1150), Expect = 3.3e-124
Identity = 301/780 (38.59%), Postives = 443/780 (56.79%), Query Frame = 0

Query: 17  GRAHEFISSSFRAFSSYMKIVSAGASTVARS----AASVASSLVDKEDEANGFQVNWAGF 76
           G+    + +SF+  SS +K VSA A+ VA S     ASVA+S+   ED+ +  QV WAGF
Sbjct: 7   GKNSGLLPNSFKIISSCLKTVSANATNVASSVRSAGASVAASISAAEDDKD--QVTWAGF 66

Query: 77  DKLEWDDNVVRRVLLLGYRSGFQVWDVEEANNVQDLVSRYDGSVSYMQVLPRPIPSMRSG 136
             LE   +V R VLLLGY++GFQV+DVE+A+N  +LVS+  G VS++Q+ P P    RSG
Sbjct: 67  GILELGQHVTRHVLLLGYQNGFQVFDVEDASNFNELVSKRGGPVSFLQMQPLP---ARSG 126

Query: 137 DK--FTESRPLLVLSAYGSITAGFKIQDRLATSGNAAVPKVQELAVAGFM--PTFVRFYS 196
           D   F  S PLL++ A G  T G  +    + +G+ A     +      +  PT VRFYS
Sbjct: 127 DHEGFWNSHPLLLVVA-GDETNGTGLGHSFSQNGSLARDGSSDSKAGDAINYPTTVRFYS 186

Query: 197 LKSQTYVHELKFRSVVYSVRCSALVVAISLFTQIHCINATTLETEHIIHTNPVASGFL-- 256
           L+S +YV+ L+FRS V  +RCS+ VVA+ L  QI+C++A TLE +  + T PV       
Sbjct: 187 LRSHSYVYVLRFRSSVCMIRCSSRVVAVGLANQIYCVDALTLENKFSVLTYPVPQPVRQG 246

Query: 257 GSGGGIGYGPLALGSRWLAYSGSPIIASNVGRVTPQHLKPSASLSHSSLNG--SLVAHYA 316
            +   +GYGP+A+G RWLAY+    +    GR++PQ    S SLS SS +G  S +A YA
Sbjct: 247 TTRVNVGYGPMAVGPRWLAYASKSSMTMKTGRLSPQTFTSSPSLSPSSSSGGSSFMARYA 306

Query: 317 KESSKQLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQHGAQGLNGIGYLKDHVADADSV 376
            ESSKQLA GL+ +GD G K LS+Y  ++LPD + S       +  +G +    +DA++ 
Sbjct: 307 MESSKQLANGLINLGDMGYKTLSKYCQDMLPDGSTS-PASPNAIWKVGGVSG--SDAENA 366

Query: 377 GMVIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITPSSSSSSN 436
           GMV VKD++S ++++QF+AH SPISALCFDPSGT+LVTAS+ G+NINVF I PS S ++ 
Sbjct: 367 GMVAVKDLVSGALVSQFKAHTSPISALCFDPSGTLLVTASVCGNNINVFQIMPSRSHNAP 426

Query: 437 SSVS-STGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGGQVN 496
             +S    +S+ HL++L RG T+A++QDI FS  S W+ I SS+GT H+F +N +G    
Sbjct: 427 GDLSYEWESSHVHLFKLHRGITSAIVQDICFSQQSQWVAIISSKGTCHIFVLNSSGSDAA 486

Query: 497 FPSTSSATRTDGLVVPARQTVWLVDSGLHMPRKQNECATGGPITLSAVSRIHHGSNGWRG 556
           F        T    +PA    W     L     Q   +    + LS VSRI + S GW  
Sbjct: 487 FQPCEGEEPTR---LPASSLPWWFTQSL--SSNQQSLSPPTAVALSVVSRIKYSSFGWLN 546

Query: 557 TVSSAAAAATGRIGSVTGAIASAFHNCKGNALHMDHGSSEARYHILVFTPTGSMIQYVLQ 616
           TVS+A  AATG++   +GA+A+ FH    + L + +  + A  HILV+TP+G ++Q+ L 
Sbjct: 547 TVSNATTAATGKVFVPSGAVAAVFHKSVTHDLQL-NSRTNALEHILVYTPSGHVVQHELL 606

Query: 617 VGL---DSAIALPISSTALDLVPEFDARVAVEAVQKWNISQKQN---------RRARDNN 676
             +        L +  T+   V E D RV VE +Q W++ ++ +         +   +  
Sbjct: 607 PSVCTESPENGLRVQKTSHVQVQEDDLRVKVEPIQWWDVCRRSDWLETEERLPKSITEKQ 666

Query: 677 VDIYGDNGALDCNKNYCGEMNGKPSVLEASGTVSKAKTCTE---EQHHLYISEAELQMHA 736
            D+   +  L  +++ C  ++      E        K+C+E   E+ H Y+S  E+++ +
Sbjct: 667 YDLETVSNHLTSHEDACLSLDMNSHFSED----KYLKSCSEKPPERSHCYLSNFEVKVTS 726

Query: 737 DRTPLWTKPEIYFQVMARDGVKGDEIDQLGETEIERIPTRMVEARSKDLVPVFNHLQSPK 769
              P+W   +I F VM  D  + D     GE EIE++P   +E + K L+PVF+H  S K
Sbjct: 727 GMLPVWQNSKISFHVM--DSPR-DSSSTGGEFEIEKVPAHELEIKQKKLLPVFDHFHSTK 764

BLAST of Carg05648 vs. ExPASy Swiss-Prot
Match: Q8H1Q5 (Autophagy-related protein 18h OS=Arabidopsis thaliana OX=3702 GN=ATG18H PE=2 SV=1)

HSP 1 Score: 418.3 bits (1074), Expect = 2.1e-115
Identity = 289/774 (37.34%), Postives = 433/774 (55.94%), Query Frame = 0

Query: 3   NDRAVKQQGTVARSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSLVDKEDEA 62
           N    K  GT       + F+ +S +  S+ ++  S+G   V  ++ASVA+SL     E 
Sbjct: 15  NHNQTKNNGT-------NGFLPNSLKFISTCIRTASSG---VRSASASVAASLSSDSHEL 74

Query: 63  NGFQVNWAGFDKLEWDDNVVRRVLLLGYRSGFQVWDVEEANNVQDLVSRYDGSVSYMQVL 122
              QV W+ FD+L   ++  + VLLLGY +GFQV D++++N+V + VSR D  V+++Q+ 
Sbjct: 75  KD-QVLWSSFDRLHTSESSFKNVLLLGYTNGFQVLDIDDSNDVTEFVSRRDDPVTFLQM- 134

Query: 123 PRPIPSMRSG-DKFTESRPLLVLSAYGSITAGFKIQDRLATSGNAAVPKVQELAVAGFMP 182
            +P+P+   G + F  S P+L+  A  +  +G      + TS + +V    E  +A   P
Sbjct: 135 -QPLPAKCDGVEGFRSSHPILLAVADEAKGSG-----PIVTSRDGSVRNGYEDPLA-LSP 194

Query: 183 TFVRFYSLKSQTYVHELKFRSVVYSVRCSALVVAISLFTQIHCINATTLETEHIIHTNPV 242
           T VRFYSL+S  YVH L+FRS VY VRCS  +VA+ L +QI+C +A TLE +  + + PV
Sbjct: 195 TVVRFYSLRSHNYVHVLRFRSTVYMVRCSPRIVAVGLGSQIYCFDALTLENKFSVLSYPV 254

Query: 243 AS-GFLG-SGGGIGYGPLALGSRWLAYSGSPIIASNVGRVTPQHL-KPSASLSHSSLNGS 302
              G  G SG  +GYGP+A+G+RWLAY+ +  ++S++GR++PQ++  P  S S S  NG+
Sbjct: 255 PQLGNQGISGVNVGYGPMAVGARWLAYASNSPLSSSIGRLSPQNVTPPGVSPSTSPNNGN 314

Query: 303 LVAHYAKESSKQLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQHGAQGLNGIGYLKDHV 362
           LVA YA ESSK LAAGL+ +GDKG K +S+Y  +L  D        + G   +G +  H 
Sbjct: 315 LVARYAMESSKHLAAGLLNLGDKGYKTISKYCQDLKHDGPGPSLSSSPG-RKVGRVGSHS 374

Query: 363 ADADSVGMVIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITPS 422
           A++D VG VIVKD  S+++I QFRAH SPISALCFDPSGT+LVTASI G+NINVF I P+
Sbjct: 375 AESDVVGTVIVKDFESRAIIAQFRAHTSPISALCFDPSGTLLVTASIHGNNINVFRIMPT 434

Query: 423 SSSSSNSSVS-STGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINP 482
            + +   + S    +S+  LY+L RG T+AVIQDI FS  S WI I SS+ T H++ ++P
Sbjct: 435 PTKNGPGAQSYDWSSSHVPLYKLHRGMTSAVIQDICFSSYSQWIAIVSSKSTCHIYVLSP 494

Query: 483 AGGQVNFPSTSSATRTDGLVVPARQTVWLVDSGLHMPRKQNECATGGPITLSAVSRIHHG 542
            GG+ N     ++      + P     W   S                +TLS VSRI   
Sbjct: 495 FGGE-NVLEIRNSQFDGPTLAPTLSLPWW--SSPSFMTTHFSYPPPASVTLSVVSRIKCN 554

Query: 543 SNGWRGTVSSAAAAATGRIGSVTGAIASAFHNCKGNALHMDHGSSEARYHILVFTPTGSM 602
           +         AA++  G+    +G +A+ FH    +       SS A  ++LV+TP+G +
Sbjct: 555 N------FFHAASSVVGKPTFPSGCLAAVFHQ---SVPQESQSSSPALDYLLVYTPSGHV 614

Query: 603 IQYVL--QVGLDSAIALPISSTALDLVPEFDARVAVEAVQKWNISQKQNRRARDNNVDIY 662
           +QY L   +G D A +   +     L  E + RV VE VQ W++ ++ +   R+ N+   
Sbjct: 615 VQYKLIPSLGGDQAESNTRNGATSGLTSEEELRVKVEPVQCWDVCRRADWPEREENI--- 674

Query: 663 GDNGALDCNKNYCGEMNGKPSVLEASGTVSKAKTCTEEQHHLYISEAELQMHADRTPLWT 722
                  C   Y G  N +   L    + S+ +T   E+HH+Y++ AE+ +++ R P+W 
Sbjct: 675 -------CGLTYGGRKNAE---LTVDTSDSEDQTKPLEKHHVYLANAEVLINSGRKPIWQ 734

Query: 723 KPEIYFQVMARDGVKGDEIDQL---GETEIERIPTRMVEARSKDLVPVFNHLQS 767
             EI F  M      G  ++     GETEI ++    V+ R KDL+PV+++  S
Sbjct: 735 NSEISFYPMYPPDSDGKNLNSHQGGGETEIGKVSANEVDIRRKDLLPVYDNFHS 743

BLAST of Carg05648 vs. ExPASy Swiss-Prot
Match: Q9H6U6 (BCAS3 microtubule associated cell migration factor OS=Homo sapiens OX=9606 GN=BCAS3 PE=1 SV=3)

HSP 1 Score: 147.9 bits (372), Expect = 5.4e-34
Identity = 126/473 (26.64%), Postives = 203/473 (42.92%), Query Frame = 0

Query: 32  SYMKIVSAGASTVARSAASVASSLVDKEDEANGFQVNWAGFDKLEWDD------------ 91
           SYM+ V      V   A S      +KE      ++ W  F+  + +D            
Sbjct: 31  SYMESVVTFLQDVVPQAYSGTPLTEEKE------KIVWVRFENADLNDTSRNLEFHEIHS 90

Query: 92  --NVVRRVLLLGYRSGFQVWDVEEANNVQDLVSRYDGSVSYMQVLPRPIPSMRSGDKFTE 151
             N    ++++GY  G QVW +  +   Q+L S   G +   ++LP P    +  D F E
Sbjct: 91  TGNEPPLLIMIGYSDGMQVWSIPISGEAQELFSVRHGPIRAARILPAPQFGAQKCDNFAE 150

Query: 152 SRPLL-VLSAYGSITAGFKIQDRLATSGNAAVPKVQELAVAGFMPTF--VRFYSLKSQTY 211
            RPLL V  + GS                           +G  P +  V  YSL++   
Sbjct: 151 KRPLLGVCKSIGS---------------------------SGTSPPYCCVDLYSLRTGEM 210

Query: 212 VHELKFRSVVYSVRCSALVVAISLFTQIHCINATTLETEHIIHTNPVASGFLGSGGGIGY 271
           V  ++F++ +Y + C+  ++ + L  +I   ++ T   +  + +            G   
Sbjct: 211 VKSIQFKTPIYDLHCNKRILVVVLQEKIAAFDSCTFTKKFFVTSCYPCP-------GPNM 270

Query: 272 GPLALGSRWLAYSGSPIIASNVGRVTPQHLKPSASLSHSSLNGSLVAHYAK---ESSKQL 331
            P+ALGSRWLAY+ + +I  +  R                  G  +  Y      ++K L
Sbjct: 271 NPIALGSRWLAYAENKLIRCHQSR--------------GGACGDNIQSYTATVISAAKTL 330

Query: 332 AAGLVTIGDKGIKKLSRYYSELLPDSNNSHQHGAQGLNGIGYLKDHVADADSVG---MVI 391
            +GL  +G    +      S +  D    H +  +     G +   V D ++VG   +++
Sbjct: 331 KSGLTMVGKVVTQLTGTLPSGVTEDDVAIHSNSRRSPLVPGIIT--VIDTETVGEGQVLV 390

Query: 392 VKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITPSSSSSSNSSVS 451
            +D  S  ++  F AH+ P+  + F+ SG +LVT    GH+ +VF I     SSS  +V 
Sbjct: 391 SEDSDSDGIVAHFPAHEKPVCCMAFNTSGMLLVTTDTLGHDFHVFQILTHPWSSSQCAV- 440

Query: 452 STGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGGQ 482
                  HLY L RG T A +QDI FS+D  W+++S+ RGTSH+F INP GGQ
Sbjct: 451 ------HHLYTLHRGETEAKVQDICFSHDCRWVVVSTLRGTSHVFPINPYGGQ 440

BLAST of Carg05648 vs. ExPASy Swiss-Prot
Match: Q8CCN5 (BCAS3 microtubule associated cell migration factor OS=Mus musculus OX=10090 GN=Bcas3 PE=1 SV=2)

HSP 1 Score: 147.1 bits (370), Expect = 9.2e-34
Identity = 127/473 (26.85%), Postives = 204/473 (43.13%), Query Frame = 0

Query: 32  SYMKIVSAGASTVARSAASVASSLVDKEDEANGFQVNWAGFDKLEWDD------------ 91
           SYM+ V      V   A S +    +KE      ++ W  F+  + +D            
Sbjct: 31  SYMESVVTFLQDVVPQAYSGSPLTEEKE------KIVWVRFENADLNDTSRNLEFHELHS 90

Query: 92  --NVVRRVLLLGYRSGFQVWDVEEANNVQDLVSRYDGSVSYMQVLPRPIPSMRSGDKFTE 151
             N    ++++GY  G QVW +  +   Q+L S   G V   ++LP P    +  D F E
Sbjct: 91  TGNEPPLLVMIGYSDGMQVWGIPISGEAQELFSVRHGPVRAARILPAPQLGAQKCDNFAE 150

Query: 152 SRPLL-VLSAYGSITAGFKIQDRLATSGNAAVPKVQELAVAGFMPTF--VRFYSLKSQTY 211
            RPLL V  + GS                           +G  P +  V  YSL++   
Sbjct: 151 KRPLLGVCKSIGS---------------------------SGTTPPYCCVDLYSLRTGEM 210

Query: 212 VHELKFRSVVYSVRCSALVVAISLFTQIHCINATTLETEHIIHTNPVASGFLGSGGGIGY 271
           V  ++F++ +Y + C+  ++ + L  +I   ++ T   +  + +            G   
Sbjct: 211 VKSIQFKTPIYDLHCNKRILVVVLQEKIAAFDSCTFTKKFFVTSCYPCP-------GPNM 270

Query: 272 GPLALGSRWLAYSGSPIIASNVGRVTPQHLKPSASLSHSSLNGSLVAHYAK---ESSKQL 331
            P+ALGSRWLAY+ + +I  +  R                  G  +  Y      ++K L
Sbjct: 271 NPIALGSRWLAYAENKLIRCHQSR--------------GGACGDNIQSYTATVLSAAKTL 330

Query: 332 AAGLVTIGDKGIKKLSRYYSELLPDSNNSHQHGAQGLNGIGYLKDHVADADSVG---MVI 391
            +GL  +G    +      S +  D    H +  +     G +   V D ++VG   +++
Sbjct: 331 KSGLTMVGKVVTQLTGTLPSGVTEDDVALHCNSRRSPLVPGIIT--VIDTETVGEGQVLV 390

Query: 392 VKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITPSSSSSSNSSVS 451
            +D  S  ++  F AH+ P+  + F+ SG +LVT    GH+ +VF I     SSS  +V 
Sbjct: 391 SEDSDSDGIVAHFPAHEKPVCCMAFNTSGMLLVTTDTLGHDFHVFQILTHPWSSSQCAV- 440

Query: 452 STGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGGQ 482
                  HLY L RG T A +QDI FS+D  W+++S+ RGTSH+F INP GGQ
Sbjct: 451 ------HHLYTLHRGETEAKVQDICFSHDCRWVVVSTLRGTSHVFPINPYGGQ 440

BLAST of Carg05648 vs. ExPASy TrEMBL
Match: A0A6J1FPV0 (autophagy-related protein 18f-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445888 PE=4 SV=1)

HSP 1 Score: 1687.2 bits (4368), Expect = 0.0e+00
Identity = 867/885 (97.97%), Postives = 871/885 (98.42%), Query Frame = 0

Query: 1   MGNDRAVKQQGTVARSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSLVDKED 60
           MGNDRAVKQQGTVARSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSLVDK+D
Sbjct: 1   MGNDRAVKQQGTVARSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSLVDKDD 60

Query: 61  EANGFQVNWAGFDKLEWDDNVVRRVLLLGYRSGFQVWDVEEANNVQDLVSRYDGSVSYMQ 120
           EANGFQVNWAGFDKLEWDDNVVRRVLLLGY SGFQVWDVEEANNVQDLVSRYDGSVSYMQ
Sbjct: 61  EANGFQVNWAGFDKLEWDDNVVRRVLLLGYCSGFQVWDVEEANNVQDLVSRYDGSVSYMQ 120

Query: 121 VLPRPIPSMRSGDKFTESRPLLVLSAYGSITAGFKIQDRLATSGNAAVPKVQELAVAGFM 180
           VLPRPIPSMRSGDKFTESRPLLVLSAYGSITAGFKIQDR+ATSGNAAVPKVQE A AGFM
Sbjct: 121 VLPRPIPSMRSGDKFTESRPLLVLSAYGSITAGFKIQDRIATSGNAAVPKVQEPADAGFM 180

Query: 181 PTFVRFYSLKSQTYVHELKFRSVVYSVRCSALVVAISLFTQIHCINATTLETEHIIHTNP 240
           PTFVRFYSLKSQTYVHELKFRSVVYSVRCS LVVAISL TQIHCINATTLETEHIIHTNP
Sbjct: 181 PTFVRFYSLKSQTYVHELKFRSVVYSVRCSPLVVAISLVTQIHCINATTLETEHIIHTNP 240

Query: 241 VASGFLGSGGGIGYGPLALGSRWLAYSGSPIIASNVGRVTPQHLKPSASLSHSSLNGSLV 300
           VASGFL SGGGIGYGPLALGSRWLAYSGSPI ASNVGRVTPQHLKPSASLSHSSLNGSLV
Sbjct: 241 VASGFLCSGGGIGYGPLALGSRWLAYSGSPITASNVGRVTPQHLKPSASLSHSSLNGSLV 300

Query: 301 AHYAKESSKQLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQHGAQGLNGIGYLKDHVAD 360
           AHYAKESSKQLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQHGAQGLNGIGYLKDHVAD
Sbjct: 301 AHYAKESSKQLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQHGAQGLNGIGYLKDHVAD 360

Query: 361 ADSVGMVIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITPSSS 420
           ADSVGMVIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITP  S
Sbjct: 361 ADSVGMVIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITP--S 420

Query: 421 SSSNSSVSSTGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGG 480
           SSSNSSVSSTGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGG
Sbjct: 421 SSSNSSVSSTGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGG 480

Query: 481 QVNFPSTSSATRTDGLVVPARQTVWLVDSGLHMPRKQNECATGGPITLSAVSRIHHGSNG 540
           QVNFPSTSSATRTDGLVVPARQTV LVDSGLHMPRKQNECAT GPITLSAVSRIHHGSNG
Sbjct: 481 QVNFPSTSSATRTDGLVVPARQTVRLVDSGLHMPRKQNECATRGPITLSAVSRIHHGSNG 540

Query: 541 WRGTVSSAAAAATGRIGSVTGAIASAFHNCKGNALHMDHGSSEARYHILVFTPTGSMIQY 600
           WRGTVSSAAAAATGRIGSVTGAIASAFHNCKGNALHMDHGSSEARYHILVFTPTGSMIQY
Sbjct: 541 WRGTVSSAAAAATGRIGSVTGAIASAFHNCKGNALHMDHGSSEARYHILVFTPTGSMIQY 600

Query: 601 VLQVGLDSAIALPISSTALDLVPEFDARVAVEAVQKWNISQKQNRRARDNNVDIYGDNGA 660
           VL+VGLDSAIALPISSTALDLVPEFDARVAVEAVQKWNISQKQNRRARDNNVDIYGDNGA
Sbjct: 601 VLRVGLDSAIALPISSTALDLVPEFDARVAVEAVQKWNISQKQNRRARDNNVDIYGDNGA 660

Query: 661 LDCNKNYCGEMNGKPSVLEASGTVSKAKTCTEEQHHLYISEAELQMHADRTPLWTKPEIY 720
           LDCNKNYC EMNGKPSVLEASGTVSKAKTCTEEQHHLYISEAELQMHADRTPLWTKPEIY
Sbjct: 661 LDCNKNYCVEMNGKPSVLEASGTVSKAKTCTEEQHHLYISEAELQMHADRTPLWTKPEIY 720

Query: 721 FQVMARDGVKGDEIDQLGETEIERIPTRMVEARSKDLVPVFNHLQSPKISQTRSLNRNSD 780
           FQVMARDGVKGDEIDQLGETEIERIPTRMVEARSKDLVPVFNHLQ PKISQTRSLNRNSD
Sbjct: 721 FQVMARDGVKGDEIDQLGETEIERIPTRMVEARSKDLVPVFNHLQLPKISQTRSLNRNSD 780

Query: 781 QLLLHQKSDLFENGRQSRRNDASPNGSVISNGPKGTGKHGYKMVTETKGVVNGYEGPRSL 840
           QLLLHQKSDLFENGRQSR NDASPNGSVISNGPKGTGKHGYK+VTETKGVVNGYEGPRSL
Sbjct: 781 QLLLHQKSDLFENGRQSRGNDASPNGSVISNGPKGTGKHGYKLVTETKGVVNGYEGPRSL 840

Query: 841 HPELDNVNNCTESLSMECCQPKSVNNHESGVEVEDDFEGICDLFD 886
           HPELDNVNNCTESLSMECCQPKSVNNHESGVEVEDDFEGICDLFD
Sbjct: 841 HPELDNVNNCTESLSMECCQPKSVNNHESGVEVEDDFEGICDLFD 883

BLAST of Carg05648 vs. ExPASy TrEMBL
Match: A0A6J1FHR8 (autophagy-related protein 18f-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111445888 PE=4 SV=1)

HSP 1 Score: 1674.4 bits (4335), Expect = 0.0e+00
Identity = 863/885 (97.51%), Postives = 867/885 (97.97%), Query Frame = 0

Query: 1   MGNDRAVKQQGTVARSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSLVDKED 60
           MGNDRAVKQQGTVARSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSLVDK+D
Sbjct: 1   MGNDRAVKQQGTVARSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSLVDKDD 60

Query: 61  EANGFQVNWAGFDKLEWDDNVVRRVLLLGYRSGFQVWDVEEANNVQDLVSRYDGSVSYMQ 120
           EANGFQVNWAGFDKLEWDDNVVRRVLLLGY SGFQVWDVEEANNVQDLVSRYDGSVSYMQ
Sbjct: 61  EANGFQVNWAGFDKLEWDDNVVRRVLLLGYCSGFQVWDVEEANNVQDLVSRYDGSVSYMQ 120

Query: 121 VLPRPIPSMRSGDKFTESRPLLVLSAYGSITAGFKIQDRLATSGNAAVPKVQELAVAGFM 180
           VLPRPIPSMRSGDKFTESRPLLVLSAYGSITAGFKIQDR+ATSGNAAVPKVQE A AGFM
Sbjct: 121 VLPRPIPSMRSGDKFTESRPLLVLSAYGSITAGFKIQDRIATSGNAAVPKVQEPADAGFM 180

Query: 181 PTFVRFYSLKSQTYVHELKFRSVVYSVRCSALVVAISLFTQIHCINATTLETEHIIHTNP 240
           PTFVRFYSLKSQTYVHELKFRSVVYSVRCS LVVAISL TQIHCINATTLETEHIIHTNP
Sbjct: 181 PTFVRFYSLKSQTYVHELKFRSVVYSVRCSPLVVAISLVTQIHCINATTLETEHIIHTNP 240

Query: 241 VASGFLGSGGGIGYGPLALGSRWLAYSGSPIIASNVGRVTPQHLKPSASLSHSSLNGSLV 300
           VASGFL SGGGIGYGPLALGSRWLAYSGSPI ASNVGRVTPQHLKPSASLSHSSLNGSLV
Sbjct: 241 VASGFLCSGGGIGYGPLALGSRWLAYSGSPITASNVGRVTPQHLKPSASLSHSSLNGSLV 300

Query: 301 AHYAKESSKQLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQHGAQGLNGIGYLKDHVAD 360
           AHYAKESSKQLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQHGAQGLNGIGYLKDHVAD
Sbjct: 301 AHYAKESSKQLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQHGAQGLNGIGYLKDHVAD 360

Query: 361 ADSVGMVIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITPSSS 420
           ADSVGMVIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITP  S
Sbjct: 361 ADSVGMVIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITP--S 420

Query: 421 SSSNSSVSSTGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGG 480
           SSSNSSVSSTGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGG
Sbjct: 421 SSSNSSVSSTGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGG 480

Query: 481 QVNFPSTSSATRTDGLVVPARQTVWLVDSGLHMPRKQNECATGGPITLSAVSRIHHGSNG 540
           QVNFPSTSSATRTDGLVVPARQTV LVDSGLHMPRKQNECAT GPITLSAVSRIHHGSNG
Sbjct: 481 QVNFPSTSSATRTDGLVVPARQTVRLVDSGLHMPRKQNECATRGPITLSAVSRIHHGSNG 540

Query: 541 WRGTVSSAAAAATGRIGSVTGAIASAFHNCKGNALHMDHGSSEARYHILVFTPTGSMIQY 600
           WRGTVSSAAAAATGRIGSVTGAIASAFHNCKGNALHMDHGSSEARYHILVFTPTGSMIQY
Sbjct: 541 WRGTVSSAAAAATGRIGSVTGAIASAFHNCKGNALHMDHGSSEARYHILVFTPTGSMIQY 600

Query: 601 VLQVGLDSAIALPISSTALDLVPEFDARVAVEAVQKWNISQKQNRRARDNNVDIYGDNGA 660
           VL+VGLDSAIALPISSTALDLVPEFDARVAVEAVQKWNISQKQNRRARDNNVDIYGDNGA
Sbjct: 601 VLRVGLDSAIALPISSTALDLVPEFDARVAVEAVQKWNISQKQNRRARDNNVDIYGDNGA 660

Query: 661 LDCNKNYCGEMNGKPSVLEASGTVSKAKTCTEEQHHLYISEAELQMHADRTPLWTKPEIY 720
           LDCNKNYC EMNGKPSVLEASGTVSKAKTCTEEQHHLYISEAELQMHADRTPLWTKPEIY
Sbjct: 661 LDCNKNYCVEMNGKPSVLEASGTVSKAKTCTEEQHHLYISEAELQMHADRTPLWTKPEIY 720

Query: 721 FQVMARDGVKGDEIDQLGETEIERIPTRMVEARSKDLVPVFNHLQSPKISQTRSLNRNSD 780
           FQVMARDGVKGDEIDQLGETEIERIPTRMVEARSKDLVPVFNHLQ PKISQT    RNSD
Sbjct: 721 FQVMARDGVKGDEIDQLGETEIERIPTRMVEARSKDLVPVFNHLQLPKISQT----RNSD 780

Query: 781 QLLLHQKSDLFENGRQSRRNDASPNGSVISNGPKGTGKHGYKMVTETKGVVNGYEGPRSL 840
           QLLLHQKSDLFENGRQSR NDASPNGSVISNGPKGTGKHGYK+VTETKGVVNGYEGPRSL
Sbjct: 781 QLLLHQKSDLFENGRQSRGNDASPNGSVISNGPKGTGKHGYKLVTETKGVVNGYEGPRSL 840

Query: 841 HPELDNVNNCTESLSMECCQPKSVNNHESGVEVEDDFEGICDLFD 886
           HPELDNVNNCTESLSMECCQPKSVNNHESGVEVEDDFEGICDLFD
Sbjct: 841 HPELDNVNNCTESLSMECCQPKSVNNHESGVEVEDDFEGICDLFD 879

BLAST of Carg05648 vs. ExPASy TrEMBL
Match: A0A6J1J1X9 (autophagy-related protein 18f-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111480521 PE=4 SV=1)

HSP 1 Score: 1629.4 bits (4218), Expect = 0.0e+00
Identity = 836/885 (94.46%), Postives = 850/885 (96.05%), Query Frame = 0

Query: 1   MGNDRAVKQQGTVARSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSLVDKED 60
           MGNDRAVKQQG VARSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSL DK+D
Sbjct: 1   MGNDRAVKQQGAVARSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSLADKDD 60

Query: 61  EANGFQVNWAGFDKLEWDDNVVRRVLLLGYRSGFQVWDVEEANNVQDLVSRYDGSVSYMQ 120
           EANGFQVNWAGFDKLEWDDNVV+RVLLLG+RSGFQVWD+EEANNVQDLVS YDGSVSYMQ
Sbjct: 61  EANGFQVNWAGFDKLEWDDNVVQRVLLLGFRSGFQVWDIEEANNVQDLVSCYDGSVSYMQ 120

Query: 121 VLPRPIPSMRSGDKFTESRPLLVLSAYGSITAGFKIQDRLATSGNAAVPKVQELAVAGFM 180
           VLPRPIP MRSGDKFTESRPLLVLSAYGSIT+GFKIQDRLATSGNAAVPKVQELA AGFM
Sbjct: 121 VLPRPIPLMRSGDKFTESRPLLVLSAYGSITSGFKIQDRLATSGNAAVPKVQELADAGFM 180

Query: 181 PTFVRFYSLKSQTYVHELKFRSVVYSVRCSALVVAISLFTQIHCINATTLETEHIIHTNP 240
           PTFVRFYSLKSQTYVHELKFRSVVYSVRCS LVVAISL TQIHCINATTLETEHIIH NP
Sbjct: 181 PTFVRFYSLKSQTYVHELKFRSVVYSVRCSPLVVAISLVTQIHCINATTLETEHIIHANP 240

Query: 241 VASGFLGSGGGIGYGPLALGSRWLAYSGSPIIASNVGRVTPQHLKPSASLSHSSLNGSLV 300
           VASGF GSGGGIGYGPLALGSRWLAYSGSPI ASNVGRVTPQHLKPSA LSHSSLNGSLV
Sbjct: 241 VASGFPGSGGGIGYGPLALGSRWLAYSGSPITASNVGRVTPQHLKPSAGLSHSSLNGSLV 300

Query: 301 AHYAKESSKQLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQHGAQGLNGIGYLKDHVAD 360
           AHYAKESSK LAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQ GA GLNGIGYLKDHVAD
Sbjct: 301 AHYAKESSKHLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQPGAHGLNGIGYLKDHVAD 360

Query: 361 ADSVGMVIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITPSSS 420
           ADSVG+VIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNI P  S
Sbjct: 361 ADSVGVVIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNIMP--S 420

Query: 421 SSSNSSVSSTGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGG 480
           SSSNS VSSTGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGG
Sbjct: 421 SSSNSPVSSTGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGG 480

Query: 481 QVNFPSTSSATRTDGLVVPARQTVWLVDSGLHMPRKQNECATGGPITLSAVSRIHHGSNG 540
           QVNFPST SATR DGLVVPARQTV LVDSGLHMPRKQNECATGGPITLSAVSRIHHGSNG
Sbjct: 481 QVNFPSTGSATRIDGLVVPARQTVRLVDSGLHMPRKQNECATGGPITLSAVSRIHHGSNG 540

Query: 541 WRGTVSSAAAAATGRIGSVTGAIASAFHNCKGNALHMDHGSSEARYHILVFTPTGSMIQY 600
           W+GTVSSAAAAATGRIGSVTGAIASAFHNCKGNALH+DHGSSEARYHILVFTPTGSMIQY
Sbjct: 541 WQGTVSSAAAAATGRIGSVTGAIASAFHNCKGNALHVDHGSSEARYHILVFTPTGSMIQY 600

Query: 601 VLQVGLDSAIALPISSTALDLVPEFDARVAVEAVQKWNISQKQNRRARDNNVDIYGDNGA 660
           VL+VGLDSAIALPI ST LDLVPE DARVAVEAVQKWNISQKQN RARDNNVDIYGDNGA
Sbjct: 601 VLRVGLDSAIALPIFSTGLDLVPELDARVAVEAVQKWNISQKQNHRARDNNVDIYGDNGA 660

Query: 661 LDCNKNYCGEMNGKPSVLEASGTVSKAKTCTEEQHHLYISEAELQMHADRTPLWTKPEIY 720
           LDC KNYCGEMNGKP VLEASGTVSKAKTCT EQHHLYISE ELQMHAD TPLWTKPEIY
Sbjct: 661 LDCKKNYCGEMNGKPIVLEASGTVSKAKTCTVEQHHLYISEVELQMHADHTPLWTKPEIY 720

Query: 721 FQVMARDGVKGDEIDQLGETEIERIPTRMVEARSKDLVPVFNHLQSPKISQTRSLNRNSD 780
           FQVMA+DGVKGDEID LGETEIERIPTRMVEARSKDLVPVFNHLQSPKISQTRSLNRNSD
Sbjct: 721 FQVMAKDGVKGDEIDNLGETEIERIPTRMVEARSKDLVPVFNHLQSPKISQTRSLNRNSD 780

Query: 781 QLLLHQKSDLFENGRQSRRNDASPNGSVISNGPKGTGKHGYKMVTETKGVVNGYEGPRSL 840
           QLLLHQKSDLFEN RQSRRNDASP+GSVI+NGPKGTGKHG+KMVTETKGVVNGY+GPRS 
Sbjct: 781 QLLLHQKSDLFENERQSRRNDASPDGSVINNGPKGTGKHGHKMVTETKGVVNGYKGPRSS 840

Query: 841 HPELDNVNNCTESLSMECCQPKSVNNHESGVEVEDDFEGICDLFD 886
           HPE DNVNNCTESLSMEC QPKSVNNHESGVEVEDDFEGICDLFD
Sbjct: 841 HPEPDNVNNCTESLSMECSQPKSVNNHESGVEVEDDFEGICDLFD 883

BLAST of Carg05648 vs. ExPASy TrEMBL
Match: A0A6J1J1Q2 (autophagy-related protein 18f-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111480521 PE=4 SV=1)

HSP 1 Score: 1616.7 bits (4185), Expect = 0.0e+00
Identity = 832/885 (94.01%), Postives = 846/885 (95.59%), Query Frame = 0

Query: 1   MGNDRAVKQQGTVARSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSLVDKED 60
           MGNDRAVKQQG VARSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSL DK+D
Sbjct: 1   MGNDRAVKQQGAVARSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSLADKDD 60

Query: 61  EANGFQVNWAGFDKLEWDDNVVRRVLLLGYRSGFQVWDVEEANNVQDLVSRYDGSVSYMQ 120
           EANGFQVNWAGFDKLEWDDNVV+RVLLLG+RSGFQVWD+EEANNVQDLVS YDGSVSYMQ
Sbjct: 61  EANGFQVNWAGFDKLEWDDNVVQRVLLLGFRSGFQVWDIEEANNVQDLVSCYDGSVSYMQ 120

Query: 121 VLPRPIPSMRSGDKFTESRPLLVLSAYGSITAGFKIQDRLATSGNAAVPKVQELAVAGFM 180
           VLPRPIP MRSGDKFTESRPLLVLSAYGSIT+GFKIQDRLATSGNAAVPKVQELA AGFM
Sbjct: 121 VLPRPIPLMRSGDKFTESRPLLVLSAYGSITSGFKIQDRLATSGNAAVPKVQELADAGFM 180

Query: 181 PTFVRFYSLKSQTYVHELKFRSVVYSVRCSALVVAISLFTQIHCINATTLETEHIIHTNP 240
           PTFVRFYSLKSQTYVHELKFRSVVYSVRCS LVVAISL TQIHCINATTLETEHIIH NP
Sbjct: 181 PTFVRFYSLKSQTYVHELKFRSVVYSVRCSPLVVAISLVTQIHCINATTLETEHIIHANP 240

Query: 241 VASGFLGSGGGIGYGPLALGSRWLAYSGSPIIASNVGRVTPQHLKPSASLSHSSLNGSLV 300
           VASGF GSGGGIGYGPLALGSRWLAYSGSPI ASNVGRVTPQHLKPSA LSHSSLNGSLV
Sbjct: 241 VASGFPGSGGGIGYGPLALGSRWLAYSGSPITASNVGRVTPQHLKPSAGLSHSSLNGSLV 300

Query: 301 AHYAKESSKQLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQHGAQGLNGIGYLKDHVAD 360
           AHYAKESSK LAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQ GA GLNGIGYLKDHVAD
Sbjct: 301 AHYAKESSKHLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQPGAHGLNGIGYLKDHVAD 360

Query: 361 ADSVGMVIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITPSSS 420
           ADSVG+VIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNI P  S
Sbjct: 361 ADSVGVVIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNIMP--S 420

Query: 421 SSSNSSVSSTGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGG 480
           SSSNS VSSTGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGG
Sbjct: 421 SSSNSPVSSTGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGG 480

Query: 481 QVNFPSTSSATRTDGLVVPARQTVWLVDSGLHMPRKQNECATGGPITLSAVSRIHHGSNG 540
           QVNFPST SATR DGLVVPARQTV LVDSGLHMPRKQNECATGGPITLSAVSRIHHGSNG
Sbjct: 481 QVNFPSTGSATRIDGLVVPARQTVRLVDSGLHMPRKQNECATGGPITLSAVSRIHHGSNG 540

Query: 541 WRGTVSSAAAAATGRIGSVTGAIASAFHNCKGNALHMDHGSSEARYHILVFTPTGSMIQY 600
           W+GTVSSAAAAATGRIGSVTGAIASAFHNCKGNALH+DHGSSEARYHILVFTPTGSMIQY
Sbjct: 541 WQGTVSSAAAAATGRIGSVTGAIASAFHNCKGNALHVDHGSSEARYHILVFTPTGSMIQY 600

Query: 601 VLQVGLDSAIALPISSTALDLVPEFDARVAVEAVQKWNISQKQNRRARDNNVDIYGDNGA 660
           VL+VGLDSAIALPI ST LDLVPE DARVAVEAVQKWNISQKQN RARDNNVDIYGDNGA
Sbjct: 601 VLRVGLDSAIALPIFSTGLDLVPELDARVAVEAVQKWNISQKQNHRARDNNVDIYGDNGA 660

Query: 661 LDCNKNYCGEMNGKPSVLEASGTVSKAKTCTEEQHHLYISEAELQMHADRTPLWTKPEIY 720
           LDC KNYCGEMNGKP VLEASGTVSKAKTCT EQHHLYISE ELQMHAD TPLWTKPEIY
Sbjct: 661 LDCKKNYCGEMNGKPIVLEASGTVSKAKTCTVEQHHLYISEVELQMHADHTPLWTKPEIY 720

Query: 721 FQVMARDGVKGDEIDQLGETEIERIPTRMVEARSKDLVPVFNHLQSPKISQTRSLNRNSD 780
           FQVMA+DGVKGDEID LGETEIERIPTRMVEARSKDLVPVFNHLQSPKISQT    RNSD
Sbjct: 721 FQVMAKDGVKGDEIDNLGETEIERIPTRMVEARSKDLVPVFNHLQSPKISQT----RNSD 780

Query: 781 QLLLHQKSDLFENGRQSRRNDASPNGSVISNGPKGTGKHGYKMVTETKGVVNGYEGPRSL 840
           QLLLHQKSDLFEN RQSRRNDASP+GSVI+NGPKGTGKHG+KMVTETKGVVNGY+GPRS 
Sbjct: 781 QLLLHQKSDLFENERQSRRNDASPDGSVINNGPKGTGKHGHKMVTETKGVVNGYKGPRSS 840

Query: 841 HPELDNVNNCTESLSMECCQPKSVNNHESGVEVEDDFEGICDLFD 886
           HPE DNVNNCTESLSMEC QPKSVNNHESGVEVEDDFEGICDLFD
Sbjct: 841 HPEPDNVNNCTESLSMECSQPKSVNNHESGVEVEDDFEGICDLFD 879

BLAST of Carg05648 vs. ExPASy TrEMBL
Match: A0A0A0LD33 (BCAS3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G415110 PE=4 SV=1)

HSP 1 Score: 1352.4 bits (3499), Expect = 0.0e+00
Identity = 701/886 (79.12%), Postives = 769/886 (86.79%), Query Frame = 0

Query: 1   MGNDRAVKQQGTVARSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSLVDKED 60
           M ND  +KQQGTVARSGRAHEF+S+SFRAFSSYMKIVSAGASTVARSAASVASSLVDK+D
Sbjct: 27  MRNDGTLKQQGTVARSGRAHEFLSTSFRAFSSYMKIVSAGASTVARSAASVASSLVDKDD 86

Query: 61  EANGFQVNWAGFDKLEWDDNVVRRVLLLGYRSGFQVWDVEEANNVQDLVSRYDGSVSYMQ 120
           EAN  QVNWAGFDKLEWDDNV+R+VLLLG RSGFQVWDVEEANNVQ+LV RYDGSVSYMQ
Sbjct: 87  EANASQVNWAGFDKLEWDDNVIRQVLLLGIRSGFQVWDVEEANNVQELVCRYDGSVSYMQ 146

Query: 121 VLPRPIPSMRSGDKFTESRPLLVLSAYGSITAGFKIQDRLATSGNAAVPKVQELAVAGFM 180
           VLPR IPSMRSGDKF ESRPL+VLSAYGSI A F IQDRLA+SGNA +PK QE+    FM
Sbjct: 147 VLPRLIPSMRSGDKFAESRPLIVLSAYGSIAANFNIQDRLASSGNATIPKSQEVVDGNFM 206

Query: 181 PTFVRFYSLKSQTYVHELKFRSVVYSVRCSALVVAISLFTQIHCINATTLETEHIIHTNP 240
           PTFVRFYSLKSQTYVHELKFRS VYSV+CS LVVAISL TQIHCINATTLE EHII TNP
Sbjct: 207 PTFVRFYSLKSQTYVHELKFRSAVYSVKCSPLVVAISLATQIHCINATTLEKEHIILTNP 266

Query: 241 VASGFLGSGGGIGYGPLALGSRWLAYSGSPIIASNVGRVTPQHLKPSASLSHSSLNGSLV 300
           V SGF GSGGG+GYGPLALG RWLAYSGSPI+ SN GRV PQHLKPSAS SHSS NGSLV
Sbjct: 267 VVSGFPGSGGGMGYGPLALGPRWLAYSGSPILLSNTGRVVPQHLKPSASFSHSSSNGSLV 326

Query: 301 AHYAKESSKQLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQHGAQGLNGIGYLKDHVAD 360
           AHYAKESSK LAAG+VT+GDKGIKKLSRYYSELLP+SNNS Q GAQGL GIG L  H+AD
Sbjct: 327 AHYAKESSKHLAAGIVTLGDKGIKKLSRYYSELLPESNNSLQSGAQGLKGIGTLNGHMAD 386

Query: 361 ADSVGMVIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITPSSS 420
           ADS+GM IVKDIISK+VITQF+AHKSPISALCFDPSGTILVTAS+QGH+INVF I P  S
Sbjct: 387 ADSIGMAIVKDIISKAVITQFKAHKSPISALCFDPSGTILVTASVQGHSINVFKIMP--S 446

Query: 421 SSSNSSVSSTGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGG 480
           S S SS+SST ASY HLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINP+GG
Sbjct: 447 SCSKSSISSTAASYSHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPSGG 506

Query: 481 QVNFPSTSSATRTDGLVVPARQTVWLVDSGLHMPRKQNECATGGPITLSAVSRIHHGSNG 540
           QVNFPS     R  G VVPARQTV  VDSGLHMP KQN+C TG P+TLSAV+RIHHGSNG
Sbjct: 507 QVNFPSADIIARNGGPVVPARQTVRRVDSGLHMPSKQNQCTTGSPLTLSAVTRIHHGSNG 566

Query: 541 WRGTVSSAAAAATGRIGSVTGAIASAFHNCKGNALHMDHGSSEARYHILVFTPTGSMIQY 600
           WRGTVSSAAAAATG++G V+GAIASAFH CKGN +H+D+GSSE RYHILVF+P+GSMIQY
Sbjct: 567 WRGTVSSAAAAATGKMGIVSGAIASAFHECKGNTVHVDNGSSEVRYHILVFSPSGSMIQY 626

Query: 601 VLQVGLDSAIALPISSTALDLVPEFDARVAVEAVQKWNISQKQNRRARDNNVDIYGDNGA 660
            L+VGLDS + LP SSTAL+LV E D+R+ VEA+QKWNISQKQNRR++DN++DIYGDNG 
Sbjct: 627 ALRVGLDSTVVLPRSSTALELVSELDSRLVVEAIQKWNISQKQNRRSQDNSIDIYGDNGG 686

Query: 661 LDCNKNYCGEMNGKPSVLEASGTVSKAKTCTEEQHHLYISEAELQMHADRTPLWTKPEIY 720
            +CNKNYC EMNG P VLEA G + K K C EEQ+HLYISEAELQMHA RTPLWTKPEIY
Sbjct: 687 FNCNKNYCEEMNGNP-VLEAGGNIFKTKACREEQYHLYISEAELQMHAARTPLWTKPEIY 746

Query: 721 FQVMARDGVKGDEIDQLGETEIERIPTRMVEARSKDLVPVFNHLQSPKISQTRSLNRNSD 780
           FQVMARDGVK DEID  GE +IERIPTRM+EARSKDLVPVF++LQS KISQ R LN NSD
Sbjct: 747 FQVMARDGVKIDEIDHPGELDIERIPTRMIEARSKDLVPVFDYLQSSKISQPRYLNSNSD 806

Query: 781 QLLLHQKSDLFENGRQSRRNDASPNGSVISNGPKGTGKHGYKMVTETKGVVNGYEGPRSL 840
           QLLL QKS  FENGRQS R+ A+P+ SV+ NG + TG+HGYK VTETKG+VN  E PRS 
Sbjct: 807 QLLLQQKSGQFENGRQSWRSAANPHDSVVGNGREVTGRHGYKRVTETKGIVNEPECPRS- 866

Query: 841 HPELDNVNNCTESLSMECCQPKSVNN-HESGVEVEDDFEGICDLFD 886
             +  NVN+CTES+SME  QPKSVNN H+SG+ VE+ FE + D F+
Sbjct: 867 QTQFSNVNSCTESISME-SQPKSVNNHHKSGLRVENHFEDLGDEFE 907

BLAST of Carg05648 vs. TAIR 10
Match: AT5G54730.1 (homolog of yeast autophagy 18 (ATG18) F )

HSP 1 Score: 515.0 bits (1325), Expect = 1.2e-145
Identity = 351/777 (45.17%), Postives = 462/777 (59.46%), Query Frame = 0

Query: 11  GTVARSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSLVDKEDEANGFQVNWA 70
           G V+RS R      SSFRA S+ +K++S+GASTVARSA S ASS V+   +    QV WA
Sbjct: 15  GVVSRSAR------SSFRALSNCLKVISSGASTVARSAVSAASSAVESHHD----QVLWA 74

Query: 71  GFDKLEWDDNVVRRVLLLGYRSGFQVWDVEEANNVQDLVSRYDGSVSYMQVLPRPIPSMR 130
           GFD L+ +D   RRVLLL ++SGFQVWDVE+  NV  +VS +DG   +MQ+L  PI S  
Sbjct: 75  GFDNLQKEDGDTRRVLLLAFKSGFQVWDVEDTENVHVIVSAHDGQAFFMQMLLNPINSGV 134

Query: 131 SGDKFTESRPLL-VLSAYGSITAGFKIQDRLATSGNAAVPKVQELAVAGFMPTFVRFYSL 190
             D+F +SRPLL V   Y S         +  +S N   P  + +A     PT V  YSL
Sbjct: 135 LDDRFYKSRPLLAVCGDYSS---------KKISSDN---PGSETVAT----PTNVYVYSL 194

Query: 191 KSQTYVHELKFRSVVYSVRCSALVVAISLFTQIHCINATTLETEHIIHTNPVASGFLGSG 250
           KSQ+YVH LKFR+ +YSVRC + +VA+    QI C +A TLE ++ I TN +  G L   
Sbjct: 195 KSQSYVHTLKFRATIYSVRCCSRIVAVLQAAQIDCFDAATLEMDYRIVTNSIVCGSL--- 254

Query: 251 GGIGYGPLALGSRWLAYSGSPIIASNVGRVTPQHLKPSASLSHSSLNGSLVAHYAKESSK 310
            G+GYGPLA+G RW+AYSGS I  S+    T +      SLS SS +   VA +A++SSK
Sbjct: 255 -GVGYGPLAVGPRWIAYSGSRIATSSSAIFTSE----IVSLSTSSPS---VAQFARDSSK 314

Query: 311 QLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQHGAQGLNGIGYLKDHVADADSVGMVIV 370
           QLA+G+  +GDKG + L++Y SE+LP+          GL GIG   + VADA+S+GMVIV
Sbjct: 315 QLASGIANLGDKGYRSLTKYCSEVLPNPY------IPGLKGIGVGNEKVADAESIGMVIV 374

Query: 371 KDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITPSSSSSSNSSVSS 430
           KDI +KSVITQF+AHKSPISALCFD SG +LVTASIQGHNINVF I P+ S+S   +V +
Sbjct: 375 KDITNKSVITQFKAHKSPISALCFDQSGLLLVTASIQGHNINVFRIMPTISTS--RAVKT 434

Query: 431 TGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGGQVNFPSTSS 490
           T  ++ HL+RLQRGFTNAVIQDI FS DSN I++ SSRGTSHLF INP            
Sbjct: 435 T--TFAHLFRLQRGFTNAVIQDICFSKDSNLIVVGSSRGTSHLFEINP------------ 494

Query: 491 ATRTDGLVVPARQTVWLVDSGLHMPRKQNECATGGPITLSAVSRIHHG-SNGWRGTVSSA 550
                                        E     P+ +SA+SRI  G S+GW GTVS A
Sbjct: 495 -----------------------------EKEGDAPVPMSAISRIRSGNSSGWIGTVSGA 554

Query: 551 AAAATGRI-GSVTGAIASAFHNCKGNALHMDHGSSE---ARYHILVFTPTGSMIQYVL-- 610
           A+AA G + GSV G + S F  C   + +  +GS     ++ ++LVF P+G M QY L  
Sbjct: 555 ASAAAGMVAGSVPGTVTSTFCYCDEKSNNNYYGSVADMCSKTNLLVFAPSGCMTQYALRE 614

Query: 611 -QVGLDSAIALPISSTALDLVP--EFDARVAVEAVQKWNISQKQNRR-ARDNNVDIYGDN 670
            QVG+    A   + T  D     E + ++AV+ +++W++ Q Q+RR   D + DIYG  
Sbjct: 615 HQVGVGHETA---AMTGFDSESGLETEGKLAVDPIRRWSMIQNQSRRETHDPHSDIYGGG 674

Query: 671 GALDCNKNYCGEMNGKPSVLEASGTVSKAKTCTEEQHHLYISEAELQMH--ADRTPLWTK 730
            ++D       E+  K SV EA     K  T   ++ HLYI EAE Q      + PLW +
Sbjct: 675 TSVDSKSKVFPEVVRKQSVEEAWKVSKKGTTRVVDKRHLYIYEAEQQQTHLPTQLPLWAR 698

Query: 731 PEIYFQVMARDGVKGDEIDQLG--ETEIERIPTRMVEARSKDLVPVFNHLQSPKISQ 772
            +  FQ +  +  +G+EI   G  E EIE I TR +EAR++DLVPV+ +LQSP+  Q
Sbjct: 735 RKFRFQELVLN--RGEEISGGGGREMEIEGIQTRTIEARTRDLVPVWGYLQSPRSQQ 698

BLAST of Carg05648 vs. TAIR 10
Match: AT1G03380.1 (homolog of yeast autophagy 18 (ATG18) G )

HSP 1 Score: 447.6 bits (1150), Expect = 2.3e-125
Identity = 301/780 (38.59%), Postives = 443/780 (56.79%), Query Frame = 0

Query: 17  GRAHEFISSSFRAFSSYMKIVSAGASTVARS----AASVASSLVDKEDEANGFQVNWAGF 76
           G+    + +SF+  SS +K VSA A+ VA S     ASVA+S+   ED+ +  QV WAGF
Sbjct: 7   GKNSGLLPNSFKIISSCLKTVSANATNVASSVRSAGASVAASISAAEDDKD--QVTWAGF 66

Query: 77  DKLEWDDNVVRRVLLLGYRSGFQVWDVEEANNVQDLVSRYDGSVSYMQVLPRPIPSMRSG 136
             LE   +V R VLLLGY++GFQV+DVE+A+N  +LVS+  G VS++Q+ P P    RSG
Sbjct: 67  GILELGQHVTRHVLLLGYQNGFQVFDVEDASNFNELVSKRGGPVSFLQMQPLP---ARSG 126

Query: 137 DK--FTESRPLLVLSAYGSITAGFKIQDRLATSGNAAVPKVQELAVAGFM--PTFVRFYS 196
           D   F  S PLL++ A G  T G  +    + +G+ A     +      +  PT VRFYS
Sbjct: 127 DHEGFWNSHPLLLVVA-GDETNGTGLGHSFSQNGSLARDGSSDSKAGDAINYPTTVRFYS 186

Query: 197 LKSQTYVHELKFRSVVYSVRCSALVVAISLFTQIHCINATTLETEHIIHTNPVASGFL-- 256
           L+S +YV+ L+FRS V  +RCS+ VVA+ L  QI+C++A TLE +  + T PV       
Sbjct: 187 LRSHSYVYVLRFRSSVCMIRCSSRVVAVGLANQIYCVDALTLENKFSVLTYPVPQPVRQG 246

Query: 257 GSGGGIGYGPLALGSRWLAYSGSPIIASNVGRVTPQHLKPSASLSHSSLNG--SLVAHYA 316
            +   +GYGP+A+G RWLAY+    +    GR++PQ    S SLS SS +G  S +A YA
Sbjct: 247 TTRVNVGYGPMAVGPRWLAYASKSSMTMKTGRLSPQTFTSSPSLSPSSSSGGSSFMARYA 306

Query: 317 KESSKQLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQHGAQGLNGIGYLKDHVADADSV 376
            ESSKQLA GL+ +GD G K LS+Y  ++LPD + S       +  +G +    +DA++ 
Sbjct: 307 MESSKQLANGLINLGDMGYKTLSKYCQDMLPDGSTS-PASPNAIWKVGGVSG--SDAENA 366

Query: 377 GMVIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITPSSSSSSN 436
           GMV VKD++S ++++QF+AH SPISALCFDPSGT+LVTAS+ G+NINVF I PS S ++ 
Sbjct: 367 GMVAVKDLVSGALVSQFKAHTSPISALCFDPSGTLLVTASVCGNNINVFQIMPSRSHNAP 426

Query: 437 SSVS-STGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINPAGGQVN 496
             +S    +S+ HL++L RG T+A++QDI FS  S W+ I SS+GT H+F +N +G    
Sbjct: 427 GDLSYEWESSHVHLFKLHRGITSAIVQDICFSQQSQWVAIISSKGTCHIFVLNSSGSDAA 486

Query: 497 FPSTSSATRTDGLVVPARQTVWLVDSGLHMPRKQNECATGGPITLSAVSRIHHGSNGWRG 556
           F        T    +PA    W     L     Q   +    + LS VSRI + S GW  
Sbjct: 487 FQPCEGEEPTR---LPASSLPWWFTQSL--SSNQQSLSPPTAVALSVVSRIKYSSFGWLN 546

Query: 557 TVSSAAAAATGRIGSVTGAIASAFHNCKGNALHMDHGSSEARYHILVFTPTGSMIQYVLQ 616
           TVS+A  AATG++   +GA+A+ FH    + L + +  + A  HILV+TP+G ++Q+ L 
Sbjct: 547 TVSNATTAATGKVFVPSGAVAAVFHKSVTHDLQL-NSRTNALEHILVYTPSGHVVQHELL 606

Query: 617 VGL---DSAIALPISSTALDLVPEFDARVAVEAVQKWNISQKQN---------RRARDNN 676
             +        L +  T+   V E D RV VE +Q W++ ++ +         +   +  
Sbjct: 607 PSVCTESPENGLRVQKTSHVQVQEDDLRVKVEPIQWWDVCRRSDWLETEERLPKSITEKQ 666

Query: 677 VDIYGDNGALDCNKNYCGEMNGKPSVLEASGTVSKAKTCTE---EQHHLYISEAELQMHA 736
            D+   +  L  +++ C  ++      E        K+C+E   E+ H Y+S  E+++ +
Sbjct: 667 YDLETVSNHLTSHEDACLSLDMNSHFSED----KYLKSCSEKPPERSHCYLSNFEVKVTS 726

Query: 737 DRTPLWTKPEIYFQVMARDGVKGDEIDQLGETEIERIPTRMVEARSKDLVPVFNHLQSPK 769
              P+W   +I F VM  D  + D     GE EIE++P   +E + K L+PVF+H  S K
Sbjct: 727 GMLPVWQNSKISFHVM--DSPR-DSSSTGGEFEIEKVPAHELEIKQKKLLPVFDHFHSTK 764

BLAST of Carg05648 vs. TAIR 10
Match: AT1G54710.1 (homolog of yeast autophagy 18 (ATG18) H )

HSP 1 Score: 418.3 bits (1074), Expect = 1.5e-116
Identity = 289/774 (37.34%), Postives = 433/774 (55.94%), Query Frame = 0

Query: 3   NDRAVKQQGTVARSGRAHEFISSSFRAFSSYMKIVSAGASTVARSAASVASSLVDKEDEA 62
           N    K  GT       + F+ +S +  S+ ++  S+G   V  ++ASVA+SL     E 
Sbjct: 15  NHNQTKNNGT-------NGFLPNSLKFISTCIRTASSG---VRSASASVAASLSSDSHEL 74

Query: 63  NGFQVNWAGFDKLEWDDNVVRRVLLLGYRSGFQVWDVEEANNVQDLVSRYDGSVSYMQVL 122
              QV W+ FD+L   ++  + VLLLGY +GFQV D++++N+V + VSR D  V+++Q+ 
Sbjct: 75  KD-QVLWSSFDRLHTSESSFKNVLLLGYTNGFQVLDIDDSNDVTEFVSRRDDPVTFLQM- 134

Query: 123 PRPIPSMRSG-DKFTESRPLLVLSAYGSITAGFKIQDRLATSGNAAVPKVQELAVAGFMP 182
            +P+P+   G + F  S P+L+  A  +  +G      + TS + +V    E  +A   P
Sbjct: 135 -QPLPAKCDGVEGFRSSHPILLAVADEAKGSG-----PIVTSRDGSVRNGYEDPLA-LSP 194

Query: 183 TFVRFYSLKSQTYVHELKFRSVVYSVRCSALVVAISLFTQIHCINATTLETEHIIHTNPV 242
           T VRFYSL+S  YVH L+FRS VY VRCS  +VA+ L +QI+C +A TLE +  + + PV
Sbjct: 195 TVVRFYSLRSHNYVHVLRFRSTVYMVRCSPRIVAVGLGSQIYCFDALTLENKFSVLSYPV 254

Query: 243 AS-GFLG-SGGGIGYGPLALGSRWLAYSGSPIIASNVGRVTPQHL-KPSASLSHSSLNGS 302
              G  G SG  +GYGP+A+G+RWLAY+ +  ++S++GR++PQ++  P  S S S  NG+
Sbjct: 255 PQLGNQGISGVNVGYGPMAVGARWLAYASNSPLSSSIGRLSPQNVTPPGVSPSTSPNNGN 314

Query: 303 LVAHYAKESSKQLAAGLVTIGDKGIKKLSRYYSELLPDSNNSHQHGAQGLNGIGYLKDHV 362
           LVA YA ESSK LAAGL+ +GDKG K +S+Y  +L  D        + G   +G +  H 
Sbjct: 315 LVARYAMESSKHLAAGLLNLGDKGYKTISKYCQDLKHDGPGPSLSSSPG-RKVGRVGSHS 374

Query: 363 ADADSVGMVIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITPS 422
           A++D VG VIVKD  S+++I QFRAH SPISALCFDPSGT+LVTASI G+NINVF I P+
Sbjct: 375 AESDVVGTVIVKDFESRAIIAQFRAHTSPISALCFDPSGTLLVTASIHGNNINVFRIMPT 434

Query: 423 SSSSSNSSVS-STGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINP 482
            + +   + S    +S+  LY+L RG T+AVIQDI FS  S WI I SS+ T H++ ++P
Sbjct: 435 PTKNGPGAQSYDWSSSHVPLYKLHRGMTSAVIQDICFSSYSQWIAIVSSKSTCHIYVLSP 494

Query: 483 AGGQVNFPSTSSATRTDGLVVPARQTVWLVDSGLHMPRKQNECATGGPITLSAVSRIHHG 542
            GG+ N     ++      + P     W   S                +TLS VSRI   
Sbjct: 495 FGGE-NVLEIRNSQFDGPTLAPTLSLPWW--SSPSFMTTHFSYPPPASVTLSVVSRIKCN 554

Query: 543 SNGWRGTVSSAAAAATGRIGSVTGAIASAFHNCKGNALHMDHGSSEARYHILVFTPTGSM 602
           +         AA++  G+    +G +A+ FH    +       SS A  ++LV+TP+G +
Sbjct: 555 N------FFHAASSVVGKPTFPSGCLAAVFHQ---SVPQESQSSSPALDYLLVYTPSGHV 614

Query: 603 IQYVL--QVGLDSAIALPISSTALDLVPEFDARVAVEAVQKWNISQKQNRRARDNNVDIY 662
           +QY L   +G D A +   +     L  E + RV VE VQ W++ ++ +   R+ N+   
Sbjct: 615 VQYKLIPSLGGDQAESNTRNGATSGLTSEEELRVKVEPVQCWDVCRRADWPEREENI--- 674

Query: 663 GDNGALDCNKNYCGEMNGKPSVLEASGTVSKAKTCTEEQHHLYISEAELQMHADRTPLWT 722
                  C   Y G  N +   L    + S+ +T   E+HH+Y++ AE+ +++ R P+W 
Sbjct: 675 -------CGLTYGGRKNAE---LTVDTSDSEDQTKPLEKHHVYLANAEVLINSGRKPIWQ 734

Query: 723 KPEIYFQVMARDGVKGDEIDQL---GETEIERIPTRMVEARSKDLVPVFNHLQS 767
             EI F  M      G  ++     GETEI ++    V+ R KDL+PV+++  S
Sbjct: 735 NSEISFYPMYPPDSDGKNLNSHQGGGETEIGKVSANEVDIRRKDLLPVYDNFHS 743

BLAST of Carg05648 vs. TAIR 10
Match: AT5G05150.1 (autophagy-related gene 18E )

HSP 1 Score: 48.9 bits (115), Expect = 2.4e-05
Identity = 33/113 (29.20%), Postives = 52/113 (46.02%), Query Frame = 0

Query: 365 GMVIVKDIISKSVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITPSSSSSSN 424
           G V V D +  +VI   +AH S I+ +     G++L TAS +G  I +FN    +     
Sbjct: 185 GQVQVHD-LRWNVIKFIKAHDSAIACMTLTLDGSLLATASTKGTLIRIFNAVDGTL---- 244

Query: 425 SSVSSTGASYRHLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAINP 478
                       L   +RG   A I +++ S +  W+  SS +GT H+F + P
Sbjct: 245 ------------LQEFRRGVERAEIYNVAISSNLKWVAASSEKGTLHVFRLRP 280

BLAST of Carg05648 vs. TAIR 10
Match: AT2G40810.1 (homolog of yeast autophagy 18C )

HSP 1 Score: 45.4 bits (106), Expect = 2.7e-04
Identity = 27/100 (27.00%), Postives = 46/100 (46.00%), Query Frame = 0

Query: 376 SVITQFRAHKSPISALCFDPSGTILVTASIQGHNINVFNITPSSSSSSNSSVSSTGASYR 435
           +++    AH S I+ +     G +L TAS +G  I +FN    +                
Sbjct: 192 NMVQIINAHDSSIACMTLTLDGLLLATASTKGTLIRIFNTMDGT---------------- 251

Query: 436 HLYRLQRGFTNAVIQDISFSYDSNWIMISSSRGTSHLFAI 476
            L  ++RG   A I  I+ S +  W+ +SS +GT H+F++
Sbjct: 252 RLQEVRRGVDRADIYSIALSPNVQWLAVSSDKGTVHIFSL 275

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7015218.10.0e+00100.00Autophagy-related protein 18f, partial [Cucurbita argyrosperma subsp. argyrosper... [more]
KAG6607820.10.0e+0099.21Autophagy-related protein 18f, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022940185.10.0e+0097.97autophagy-related protein 18f-like isoform X1 [Cucurbita moschata][more]
XP_022940186.10.0e+0097.51autophagy-related protein 18f-like isoform X2 [Cucurbita moschata][more]
XP_023524854.10.0e+0096.72autophagy-related protein 18f-like isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9FH321.7e-14445.17Autophagy-related protein 18f OS=Arabidopsis thaliana OX=3702 GN=ATG18F PE=2 SV=... [more]
Q8GUL13.3e-12438.59Autophagy-related protein 18g OS=Arabidopsis thaliana OX=3702 GN=ATG18G PE=2 SV=... [more]
Q8H1Q52.1e-11537.34Autophagy-related protein 18h OS=Arabidopsis thaliana OX=3702 GN=ATG18H PE=2 SV=... [more]
Q9H6U65.4e-3426.64BCAS3 microtubule associated cell migration factor OS=Homo sapiens OX=9606 GN=BC... [more]
Q8CCN59.2e-3426.85BCAS3 microtubule associated cell migration factor OS=Mus musculus OX=10090 GN=B... [more]
Match NameE-valueIdentityDescription
A0A6J1FPV00.0e+0097.97autophagy-related protein 18f-like isoform X1 OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1FHR80.0e+0097.51autophagy-related protein 18f-like isoform X2 OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1J1X90.0e+0094.46autophagy-related protein 18f-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1J1Q20.0e+0094.01autophagy-related protein 18f-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A0A0LD330.0e+0079.12BCAS3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G415110 PE=4 ... [more]
Match NameE-valueIdentityDescription
AT5G54730.11.2e-14545.17homolog of yeast autophagy 18 (ATG18) F [more]
AT1G03380.12.3e-12538.59homolog of yeast autophagy 18 (ATG18) G [more]
AT1G54710.11.5e-11637.34homolog of yeast autophagy 18 (ATG18) H [more]
AT5G05150.12.4e-0529.20autophagy-related gene 18E [more]
AT2G40810.12.7e-0427.00homolog of yeast autophagy 18C [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001680WD40 repeatSMARTSM00320WD40_4coord: 374..414
e-value: 3.1E-5
score: 33.4
coord: 433..474
e-value: 79.0
score: 5.0
IPR022175BCAS3 domainPFAMPF12490BCAS3coord: 524..759
e-value: 2.0E-70
score: 237.0
IPR015943WD40/YVTN repeat-like-containing domain superfamilyGENE3D2.130.10.10coord: 353..508
e-value: 1.6E-10
score: 42.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 790..821
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 797..812
NoneNo IPR availablePANTHERPTHR13268:SF7AUTOPHAGY-RELATED PROTEIN 18Fcoord: 15..829
IPR045142BCAS3-likePANTHERPTHR13268BREAST CARCINOMA AMPLIFIED SEQUENCE 3coord: 15..829
IPR036322WD40-repeat-containing domain superfamilySUPERFAMILY50978WD40 repeat-likecoord: 84..476

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg05648-RACarg05648-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006914 autophagy
biological_process GO:0042594 response to starvation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005515 protein binding