Carg05621 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg05621
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	E3 ubiquitin-protein ligase RHA1B-like
Location	Carg_Chr17: 2753942 .. 2754427 (+)
RNA-Seq Expression	Carg05621
Synteny	Carg05621

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCTTTCCGGTGAGTTACACGGAGGAGCTCTTCCCCAGCATCCTCCTCCTTCTGCTCACCGTTCACGACGTCCTGACTCACCTCGTCGTGTCCTGTTTCCGCCTCCTGTGGCTGCCGCAGTTTCTCCGCCGTGAATCCACCCCGCCGGAGATCCAATTTCCTCTAAACCCTCCTCCGTCAGCCGTCATTCTCCGCGAATTCCTCCCGGTGGTGAAACTCTCGGATCTTTCCGACCCGCCGGAGAATTGCGTTGTTTGCTTCTACGAGTTCGATGGCGCGGAGGAGATTCGGTTGCTGATGAACTGCAAGCACATATTCCACCGGAGGTGTGTGGAGCGTTGGATCAATCACAATCACGACACGTGTCCACTCTGTAGAACGCCGTTTGTGCCTGATGGAATGGTTGATGAACTCAATCAACGGCTGTCGGCCGCCTGTGGAACCGCTGAGTTGGCGGCGGCGTTCAACTCCGTTTCAGGGTAG

mRNA sequence

ATGGGCTTTCCGGTGAGTTACACGGAGGAGCTCTTCCCCAGCATCCTCCTCCTTCTGCTCACCGTTCACGACGTCCTGACTCACCTCGTCGTGTCCTGTTTCCGCCTCCTGTGGCTGCCGCAGTTTCTCCGCCGTGAATCCACCCCGCCGGAGATCCAATTTCCTCTAAACCCTCCTCCGTCAGCCGTCATTCTCCGCGAATTCCTCCCGGTGGTGAAACTCTCGGATCTTTCCGACCCGCCGGAGAATTGCGTTGTTTGCTTCTACGAGTTCGATGGCGCGGAGGAGATTCGGTTGCTGATGAACTGCAAGCACATATTCCACCGGAGGTGTGTGGAGCGTTGGATCAATCACAATCACGACACGTGTCCACTCTGTAGAACGCCGTTTGTGCCTGATGGAATGGTTGATGAACTCAATCAACGGCTGTCGGCCGCCTGTGGAACCGCTGAGTTGGCGGCGGCGTTCAACTCCGTTTCAGGGTAG

Coding sequence (CDS)

ATGGGCTTTCCGGTGAGTTACACGGAGGAGCTCTTCCCCAGCATCCTCCTCCTTCTGCTCACCGTTCACGACGTCCTGACTCACCTCGTCGTGTCCTGTTTCCGCCTCCTGTGGCTGCCGCAGTTTCTCCGCCGTGAATCCACCCCGCCGGAGATCCAATTTCCTCTAAACCCTCCTCCGTCAGCCGTCATTCTCCGCGAATTCCTCCCGGTGGTGAAACTCTCGGATCTTTCCGACCCGCCGGAGAATTGCGTTGTTTGCTTCTACGAGTTCGATGGCGCGGAGGAGATTCGGTTGCTGATGAACTGCAAGCACATATTCCACCGGAGGTGTGTGGAGCGTTGGATCAATCACAATCACGACACGTGTCCACTCTGTAGAACGCCGTTTGTGCCTGATGGAATGGTTGATGAACTCAATCAACGGCTGTCGGCCGCCTGTGGAACCGCTGAGTTGGCGGCGGCGTTCAACTCCGTTTCAGGGTAG

Protein sequence

MGFPVSYTEELFPSILLLLLTVHDVLTHLVVSCFRLLWLPQFLRRESTPPEIQFPLNPPPSAVILREFLPVVKLSDLSDPPENCVVCFYEFDGAEEIRLLMNCKHIFHRRCVERWINHNHDTCPLCRTPFVPDGMVDELNQRLSAACGTAELAAAFNSVSG

Homology

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BLAST of Carg05621 vs. NCBI nr
Match: KAG7013755.1 (E3 ubiquitin-protein ligase RHA1B, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 337.4 bits (864), Expect = 6.7e-89
Identity = 161/161 (100.00%), Postives = 161/161 (100.00%), Query Frame = 0


Query: 1   MGFPVSYTEELFPSILLLLLTVHDVLTHLVVSCFRLLWLPQFLRRESTPPEIQFPLNPPP 60
           MGFPVSYTEELFPSILLLLLTVHDVLTHLVVSCFRLLWLPQFLRRESTPPEIQFPLNPPP
Sbjct: 1   MGFPVSYTEELFPSILLLLLTVHDVLTHLVVSCFRLLWLPQFLRRESTPPEIQFPLNPPP 60

Query: 61  SAVILREFLPVVKLSDLSDPPENCVVCFYEFDGAEEIRLLMNCKHIFHRRCVERWINHNH 120
           SAVILREFLPVVKLSDLSDPPENCVVCFYEFDGAEEIRLLMNCKHIFHRRCVERWINHNH
Sbjct: 61  SAVILREFLPVVKLSDLSDPPENCVVCFYEFDGAEEIRLLMNCKHIFHRRCVERWINHNH 120

Query: 121 DTCPLCRTPFVPDGMVDELNQRLSAACGTAELAAAFNSVSG 162
           DTCPLCRTPFVPDGMVDELNQRLSAACGTAELAAAFNSVSG
Sbjct: 121 DTCPLCRTPFVPDGMVDELNQRLSAACGTAELAAAFNSVSG 161

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BLAST of Carg05621 vs. NCBI nr
Match: KAG6575192.1 (Brassinosteroid-responsive RING protein 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 335.1 bits (858), Expect = 3.3e-88
Identity = 160/161 (99.38%), Postives = 160/161 (99.38%), Query Frame = 0


Query: 1   MGFPVSYTEELFPSILLLLLTVHDVLTHLVVSCFRLLWLPQFLRRESTPPEIQFPLNPPP 60
           MGFPVSYTEELFPSILLLLLTVHDVLTHLVVSCFRLLWLP FLRRESTPPEIQFPLNPPP
Sbjct: 1   MGFPVSYTEELFPSILLLLLTVHDVLTHLVVSCFRLLWLPPFLRRESTPPEIQFPLNPPP 60

Query: 61  SAVILREFLPVVKLSDLSDPPENCVVCFYEFDGAEEIRLLMNCKHIFHRRCVERWINHNH 120
           SAVILREFLPVVKLSDLSDPPENCVVCFYEFDGAEEIRLLMNCKHIFHRRCVERWINHNH
Sbjct: 61  SAVILREFLPVVKLSDLSDPPENCVVCFYEFDGAEEIRLLMNCKHIFHRRCVERWINHNH 120

Query: 121 DTCPLCRTPFVPDGMVDELNQRLSAACGTAELAAAFNSVSG 162
           DTCPLCRTPFVPDGMVDELNQRLSAACGTAELAAAFNSVSG
Sbjct: 121 DTCPLCRTPFVPDGMVDELNQRLSAACGTAELAAAFNSVSG 161

[Close]

BLAST of Carg05621 vs. NCBI nr
Match: XP_022958975.1 (E3 ubiquitin-protein ligase RHA1B-like [Cucurbita moschata])

HSP 1 Score: 328.2 bits (840), Expect = 4.1e-86
Identity = 157/161 (97.52%), Postives = 157/161 (97.52%), Query Frame = 0


Query: 1   MGFPVSYTEELFPSILLLLLTVHDVLTHLVVSCFRLLWLPQFLRRESTPPEIQFPLNPPP 60
           MGFPVSYTEELFPSILLLLLTVHDVLTHLVVSCFRLLWLP FLRRESTPPEIQFPLNPPP
Sbjct: 1   MGFPVSYTEELFPSILLLLLTVHDVLTHLVVSCFRLLWLPPFLRRESTPPEIQFPLNPPP 60

Query: 61  SAVILREFLPVVKLSDLSDPPENCVVCFYEFDGAEEIRLLMNCKHIFHRRCVERWINHNH 120
           SAVILREFLPVVKLSDLSDPPENCVVCFYEFDGAEEIRLLMNCKHIFHRRCVERWINHNH
Sbjct: 61  SAVILREFLPVVKLSDLSDPPENCVVCFYEFDGAEEIRLLMNCKHIFHRRCVERWINHNH 120

Query: 121 DTCPLCRTPFVPDGMVDELNQRLSAACGTAELAAAFNSVSG 162
           DTCPLCRTPFVPD MVDELNQRLSAACGTAELAA  NSVSG
Sbjct: 121 DTCPLCRTPFVPDAMVDELNQRLSAACGTAELAAEVNSVSG 161

[Close]

BLAST of Carg05621 vs. NCBI nr
Match: XP_023006675.1 (E3 ubiquitin-protein ligase RHA1B-like [Cucurbita maxima])

HSP 1 Score: 317.8 bits (813), Expect = 5.5e-83
Identity = 150/161 (93.17%), Postives = 155/161 (96.27%), Query Frame = 0


Query: 1   MGFPVSYTEELFPSILLLLLTVHDVLTHLVVSCFRLLWLPQFLRRESTPPEIQFPLNPPP 60
           MGFPV+YTEELFPS+LLLLLTVHDVLTHLV+SCFRLLWLP FLRRESTPPEIQFPLNPPP
Sbjct: 1   MGFPVTYTEELFPSMLLLLLTVHDVLTHLVMSCFRLLWLPPFLRRESTPPEIQFPLNPPP 60

Query: 61  SAVILREFLPVVKLSDLSDPPENCVVCFYEFDGAEEIRLLMNCKHIFHRRCVERWINHNH 120
           SAVILREFLPVVK SDLSDPPENCVVC YEF+GAEEIRLLMNCKHIFHRRCVERWINHNH
Sbjct: 61  SAVILREFLPVVKFSDLSDPPENCVVCLYEFEGAEEIRLLMNCKHIFHRRCVERWINHNH 120

Query: 121 DTCPLCRTPFVPDGMVDELNQRLSAACGTAELAAAFNSVSG 162
           DTCPLCRTPFVPDGMVDE NQRLSAAC TAELAA F+SVSG
Sbjct: 121 DTCPLCRTPFVPDGMVDEFNQRLSAACRTAELAAEFSSVSG 161

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BLAST of Carg05621 vs. NCBI nr
Match: XP_023549219.1 (E3 ubiquitin-protein ligase RHA1B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 315.1 bits (806), Expect = 3.5e-82
Identity = 153/162 (94.44%), Postives = 156/162 (96.30%), Query Frame = 0


Query: 1   MGFPVSYTEELFPSILLLLLTVHDVLTHLVVSCFRLLWLPQFLRRESTPPEIQFPLNPPP 60
           MGFPV+YTEELFPS+LLLLLTV+DVLTHLVVSCFRLLWLP FLRREST PEIQFP NPPP
Sbjct: 1   MGFPVTYTEELFPSVLLLLLTVNDVLTHLVVSCFRLLWLPPFLRRESTLPEIQFPPNPPP 60

Query: 61  SAVILREFLPVVKLSDLSDPPENCVVCFYEFDGAEEIRLLMNCKHIFHRRCVERWINHNH 120
           SAVILREFLPVVKLSDLSDPPENCVVCFYEFDGAEEIRLLMNCKHIFHRRCVERWINHNH
Sbjct: 61  SAVILREFLPVVKLSDLSDPPENCVVCFYEFDGAEEIRLLMNCKHIFHRRCVERWINHNH 120

Query: 121 DTCPLCRTPFVPDGMVDELNQRLSAACGT-AELAAAFNSVSG 162
           DTCPLCRTPFVPDGMVDELNQRLSAACGT AELAA  NSVSG
Sbjct: 121 DTCPLCRTPFVPDGMVDELNQRLSAACGTAAELAAEVNSVSG 162

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BLAST of Carg05621 vs. ExPASy Swiss-Prot
Match: Q9XF92 (Brassinosteroid-responsive RING protein 1 OS=Arabidopsis thaliana OX=3702 GN=BRH1 PE=2 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 1.2e-37
Identity = 78/158 (49.37%), Postives = 102/158 (64.56%), Query Frame = 0


Query: 1   MGFPVSYTEELFPSILLLLLTVHDVLTHLVVSCFRLLWLPQFLRRESTPPE-IQFPLNPP 60
           MGFPV YTE   P + +  L++   +  +V S FR L L  FL  + T P+   +P   P
Sbjct: 1   MGFPVGYTEVFLPKLFVQTLSILGFIRTIVFSIFRFLGLSDFLEMDQTWPDYTSYPTRIP 60

Query: 61  P-----SAVILREFLPVVKLSDLS----DPPENCVVCFYEFDGAEEIRLLMNCKHIFHRR 120
                 SA+++RE LPV+K  +L+    D PENC VC YEF+G +EIR L NC+HIFHR 
Sbjct: 61  ETRSPFSALLIREILPVIKFEELTNSGEDLPENCAVCLYEFEGEQEIRWLRNCRHIFHRS 120

Query: 121 CVERWINHNHDTCPLCRTPFVPDGMVDELNQRLSAACG 149
           C++RW++H+  TCPLCRTPFVPD M +E NQRL AA G
Sbjct: 121 CLDRWMDHDQKTCPLCRTPFVPDEMQEEFNQRLWAASG 158

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BLAST of Carg05621 vs. ExPASy Swiss-Prot
Match: Q9SUS5 (E3 ubiquitin-protein ligase RHA1B OS=Arabidopsis thaliana OX=3702 GN=RHA1B PE=2 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 3.2e-17
Identity = 50/135 (37.04%), Postives = 70/135 (51.85%), Query Frame = 0


Query: 1   MGFPVSYTEELFPSILLLLLTVHDVLTHLVVSCFRLLWLPQFLRRESTPPEIQFPLNPPP 60
           MG P  + E   P  +L  L V      +V +    + LP FL    T       L    
Sbjct: 1   MGLPTDFKELQIPGYVLKTLYVIGFFRDMVDALCPYIGLPSFLDHNETSRSDPTRLALST 60

Query: 61  SAVILREFLPVVKLSD-LSDPPENCVVCFYEFDGAEEIRLLMNCKHIFHRRCVERWI-NH 120
           SA +  E +PVV+ SD L+DP + C VC  +F   ++IR L  C H+FH RC++RWI + 
Sbjct: 61  SATLANELIPVVRFSDLLTDPEDCCTVCLSDFVSDDKIRQLPKCGHVFHHRCLDRWIVDC 120

Query: 121 NHDTCPLCRTPFVPD 134
           N  TCP+CR  F+P+
Sbjct: 121 NKITCPICRNRFLPE 135

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BLAST of Carg05621 vs. ExPASy Swiss-Prot
Match: Q9SUS4 (Probable E3 ubiquitin-protein ligase RHA1A OS=Arabidopsis thaliana OX=3702 GN=RHA1A PE=2 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 7.8e-16
Identity = 50/137 (36.50%), Postives = 80/137 (58.39%), Query Frame = 0


Query: 1   MGFPVSYTEEL-FPSILLLLLTVHDVLTHLVVSCFRLLWLPQFL-RRESTPPEI-QFPLN 60
           MG P  +  EL  PS +L +L V      +V +    + LP+FL   E++ P++ +  L+
Sbjct: 1   MGLPEDFITELQIPSYILKILYVIGFFRDIVDALCPYIGLPRFLDHNETSAPDLTRHALS 60

Query: 61  PPPSAVILREFLPVVKLSDL-SDPPENCVVCFYEFDGAEEIRLLMNCKHIFHRRCVERWI 120
              SA +  E +PVV+ SDL +DP + C VC  +F+  +++R L  C H+FH  C++RWI
Sbjct: 61  --TSASLANELIPVVRFSDLPTDPEDCCTVCLSDFESDDKVRQLPKCGHVFHHYCLDRWI 120

Query: 121 -NHNHDTCPLCRTPFVP 133
            ++N   CP+CR  F+P
Sbjct: 121 VDYNKMKCPVCRHRFLP 135

[Close]

BLAST of Carg05621 vs. ExPASy Swiss-Prot
Match: Q5EAE9 (RING-H2 finger protein ATL43 OS=Arabidopsis thaliana OX=3702 GN=ATL43 PE=2 SV=2)

HSP 1 Score: 63.2 bits (152), Expect = 3.2e-09
Identity = 30/72 (41.67%), Postives = 40/72 (55.56%), Query Frame = 0


Query: 67  EFLPVVKLSDLSDPPE--NCVVCFYEFDGAEEIRLLMNCKHIFHRRCVERWINHNHDTCP 126
           E LPV +   LS   +   C VC   F+  E +RLL  CKH FH  CV+ W++  H TCP
Sbjct: 127 ESLPVFRFGALSGHKDGLECAVCLARFEPTEVLRLLPKCKHAFHVECVDTWLD-AHSTCP 186

Query: 127 LCRTPFVPDGMV 137
           LCR    P+ ++
Sbjct: 187 LCRYRVDPEDIL 197

[Close]

BLAST of Carg05621 vs. ExPASy Swiss-Prot
Match: Q9ZU51 (E3 ubiquitin-protein ligase RHA2B OS=Arabidopsis thaliana OX=3702 GN=RHA2B PE=1 SV=2)

HSP 1 Score: 63.2 bits (152), Expect = 3.2e-09
Identity = 22/50 (44.00%), Postives = 36/50 (72.00%), Query Frame = 0


Query: 83  NCVVCFYEFDGAEEIRLLMNCKHIFHRRCVERWINHNHDTCPLCRTPFVP 133
           +C+VC  +    EE+R L +C+H+FH++C+E W+ H +  CPLCR+P +P
Sbjct: 73  DCIVCLSKLKTGEEVRKL-DCRHVFHKQCLEGWLQHLNFNCPLCRSPLLP 121

[Close]

BLAST of Carg05621 vs. ExPASy TrEMBL
Match: A0A6J1H500 (E3 ubiquitin-protein ligase RHA1B-like OS=Cucurbita moschata OX=3662 GN=LOC111460104 PE=4 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 2.0e-86
Identity = 157/161 (97.52%), Postives = 157/161 (97.52%), Query Frame = 0


Query: 1   MGFPVSYTEELFPSILLLLLTVHDVLTHLVVSCFRLLWLPQFLRRESTPPEIQFPLNPPP 60
           MGFPVSYTEELFPSILLLLLTVHDVLTHLVVSCFRLLWLP FLRRESTPPEIQFPLNPPP
Sbjct: 1   MGFPVSYTEELFPSILLLLLTVHDVLTHLVVSCFRLLWLPPFLRRESTPPEIQFPLNPPP 60

Query: 61  SAVILREFLPVVKLSDLSDPPENCVVCFYEFDGAEEIRLLMNCKHIFHRRCVERWINHNH 120
           SAVILREFLPVVKLSDLSDPPENCVVCFYEFDGAEEIRLLMNCKHIFHRRCVERWINHNH
Sbjct: 61  SAVILREFLPVVKLSDLSDPPENCVVCFYEFDGAEEIRLLMNCKHIFHRRCVERWINHNH 120

Query: 121 DTCPLCRTPFVPDGMVDELNQRLSAACGTAELAAAFNSVSG 162
           DTCPLCRTPFVPD MVDELNQRLSAACGTAELAA  NSVSG
Sbjct: 121 DTCPLCRTPFVPDAMVDELNQRLSAACGTAELAAEVNSVSG 161

[Close]

BLAST of Carg05621 vs. ExPASy TrEMBL
Match: A0A6J1L0T4 (E3 ubiquitin-protein ligase RHA1B-like OS=Cucurbita maxima OX=3661 GN=LOC111499330 PE=4 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 2.7e-83
Identity = 150/161 (93.17%), Postives = 155/161 (96.27%), Query Frame = 0


Query: 1   MGFPVSYTEELFPSILLLLLTVHDVLTHLVVSCFRLLWLPQFLRRESTPPEIQFPLNPPP 60
           MGFPV+YTEELFPS+LLLLLTVHDVLTHLV+SCFRLLWLP FLRRESTPPEIQFPLNPPP
Sbjct: 1   MGFPVTYTEELFPSMLLLLLTVHDVLTHLVMSCFRLLWLPPFLRRESTPPEIQFPLNPPP 60

Query: 61  SAVILREFLPVVKLSDLSDPPENCVVCFYEFDGAEEIRLLMNCKHIFHRRCVERWINHNH 120
           SAVILREFLPVVK SDLSDPPENCVVC YEF+GAEEIRLLMNCKHIFHRRCVERWINHNH
Sbjct: 61  SAVILREFLPVVKFSDLSDPPENCVVCLYEFEGAEEIRLLMNCKHIFHRRCVERWINHNH 120

Query: 121 DTCPLCRTPFVPDGMVDELNQRLSAACGTAELAAAFNSVSG 162
           DTCPLCRTPFVPDGMVDE NQRLSAAC TAELAA F+SVSG
Sbjct: 121 DTCPLCRTPFVPDGMVDEFNQRLSAACRTAELAAEFSSVSG 161

[Close]

BLAST of Carg05621 vs. ExPASy TrEMBL
Match: A0A5D3BV35 (E3 ubiquitin-protein ligase RHA1B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G001290 PE=4 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 1.5e-57
Identity = 110/162 (67.90%), Postives = 130/162 (80.25%), Query Frame = 0


Query: 1   MGFPVSYTEELFPSILLLLLTVHDVLTHLVVSCFRLLWLPQFLRRESTPPEIQFPLNPPP 60
           MGFPV  TE  FP+I L LL++ D+L  L++S FRLL LP+FL  ++ PPE+QFPLNPPP
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKFLILSLFRLLGLPEFLPSDNLPPELQFPLNPPP 60

Query: 61  SAVILREFLPVVKLSDLSDPPENCVVCFYEFDGAEEIRLLMNCKHIFHRRCVERWINHNH 120
           SA++LRE LPVVK SDL +PPE C VC YEF+GAEEIR L NCKHIFHRRC++RW++H+H
Sbjct: 61  SALLLRELLPVVKFSDLPNPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCLDRWMDHDH 120

Query: 121 DTCPLCRTPFVPDGMVDELNQRLSAACGTAE-LAAAFNSVSG 162
           DTCPLCRTPFVPDGM+DE NQRL AA G AE     FNSV G
Sbjct: 121 DTCPLCRTPFVPDGMMDEFNQRLWAASGVAETFETDFNSVLG 162

[Close]

BLAST of Carg05621 vs. ExPASy TrEMBL
Match: A0A1S3C782 (E3 ubiquitin-protein ligase RHA1B OS=Cucumis melo OX=3656 GN=LOC103497696 PE=4 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 1.5e-57
Identity = 110/162 (67.90%), Postives = 130/162 (80.25%), Query Frame = 0


Query: 1   MGFPVSYTEELFPSILLLLLTVHDVLTHLVVSCFRLLWLPQFLRRESTPPEIQFPLNPPP 60
           MGFPV  TE  FP+I L LL++ D+L  L++S FRLL LP+FL  ++ PPE+QFPLNPPP
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKFLILSLFRLLGLPEFLPSDNLPPELQFPLNPPP 60

Query: 61  SAVILREFLPVVKLSDLSDPPENCVVCFYEFDGAEEIRLLMNCKHIFHRRCVERWINHNH 120
           SA++LRE LPVVK SDL +PPE C VC YEF+GAEEIR L NCKHIFHRRC++RW++H+H
Sbjct: 61  SALLLRELLPVVKFSDLPNPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCLDRWMDHDH 120

Query: 121 DTCPLCRTPFVPDGMVDELNQRLSAACGTAE-LAAAFNSVSG 162
           DTCPLCRTPFVPDGM+DE NQRL AA G AE     FNSV G
Sbjct: 121 DTCPLCRTPFVPDGMMDEFNQRLWAASGVAETFETDFNSVLG 162

[Close]

BLAST of Carg05621 vs. ExPASy TrEMBL
Match: A0A0A0K874 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G006705 PE=4 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 1.6e-56
Identity = 110/162 (67.90%), Postives = 128/162 (79.01%), Query Frame = 0


Query: 1   MGFPVSYTEELFPSILLLLLTVHDVLTHLVVSCFRLLWLPQFLRRESTPPEIQFPLNPPP 60
           MGFPV  TE  FP+I L LL++ DVL  L++S FRLL LP+FL  ++ PPE+QFPLNPPP
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDVLKFLILSLFRLLGLPEFLPSDNLPPELQFPLNPPP 60

Query: 61  SAVILREFLPVVKLSDLSDPPENCVVCFYEFDGAEEIRLLMNCKHIFHRRCVERWINHNH 120
           SA++LRE LPVVK SDL +PPE C VC YEF+GAEEIR L NCKHIFHRRCV+ W++H+H
Sbjct: 61  SALLLRELLPVVKFSDLPNPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCVDSWMDHDH 120

Query: 121 DTCPLCRTPFVPDGMVDELNQRLSAACGTAE-LAAAFNSVSG 162
           DTCPLCRTPFVPD M+DE NQRL AA G AE     FNSV G
Sbjct: 121 DTCPLCRTPFVPDEMMDEFNQRLWAASGIAETFETDFNSVLG 162

[Close]

BLAST of Carg05621 vs. TAIR 10
Match: AT3G61460.1 (brassinosteroid-responsive RING-H2 )

HSP 1 Score: 157.5 bits (397), Expect = 8.8e-39
Identity = 78/158 (49.37%), Postives = 102/158 (64.56%), Query Frame = 0


Query: 1   MGFPVSYTEELFPSILLLLLTVHDVLTHLVVSCFRLLWLPQFLRRESTPPE-IQFPLNPP 60
           MGFPV YTE   P + +  L++   +  +V S FR L L  FL  + T P+   +P   P
Sbjct: 1   MGFPVGYTEVFLPKLFVQTLSILGFIRTIVFSIFRFLGLSDFLEMDQTWPDYTSYPTRIP 60

Query: 61  P-----SAVILREFLPVVKLSDLS----DPPENCVVCFYEFDGAEEIRLLMNCKHIFHRR 120
                 SA+++RE LPV+K  +L+    D PENC VC YEF+G +EIR L NC+HIFHR 
Sbjct: 61  ETRSPFSALLIREILPVIKFEELTNSGEDLPENCAVCLYEFEGEQEIRWLRNCRHIFHRS 120

Query: 121 CVERWINHNHDTCPLCRTPFVPDGMVDELNQRLSAACG 149
           C++RW++H+  TCPLCRTPFVPD M +E NQRL AA G
Sbjct: 121 CLDRWMDHDQKTCPLCRTPFVPDEMQEEFNQRLWAASG 158

[Close]

BLAST of Carg05621 vs. TAIR 10
Match: AT1G63840.1 (RING/U-box superfamily protein )

HSP 1 Score: 132.1 bits (331), Expect = 4.0e-31
Identity = 69/152 (45.39%), Postives = 96/152 (63.16%), Query Frame = 0


Query: 1   MGFPVSYTEELFPSILLLLLTVHDVLTHLVVSCFRLLWLPQFLRRESTPPEIQFPLNPPP 60
           MGFPV Y+E L P I   LL+   ++  L+ + F+++ LP FL  E  P    +P +PPP
Sbjct: 1   MGFPVGYSELLLPKIFFYLLSFLGLIRKLISTMFKIIGLPDFL--EPEPVSTSWP-DPPP 60

Query: 61  -------SAVILREFLPVVKLSDLSDP-PENCVVCFYEFDGAEEIRLLMNCKHIFHRRCV 120
                  +A++  E LPVV+ SD++ P  E C VC Y+F+  +EIR L NC+HIFHR C+
Sbjct: 61  TLTKPDSAAILAGEMLPVVRFSDINRPESECCAVCLYDFENDDEIRRLTNCRHIFHRGCL 120

Query: 121 ERW-INHNHDTCPLCRTPFVPDGMVDELNQRL 144
           +RW + +N  TCPLCRT F+PD +  E NQRL
Sbjct: 121 DRWMMGYNQMTCPLCRTQFIPDHLQLEFNQRL 149

[Close]

BLAST of Carg05621 vs. TAIR 10
Match: AT5G41400.1 (RING/U-box superfamily protein )

HSP 1 Score: 116.7 bits (291), Expect = 1.7e-26
Identity = 65/165 (39.39%), Postives = 94/165 (56.97%), Query Frame = 0


Query: 1   MGFPVSYTEELFPSILLLLLTVHDVLTHLVVSCFRLLWLPQFLRREST--------PPEI 60
           MG+PV YTE L P I L LL++  ++  L+ + FR+L LP FL  +          PP +
Sbjct: 1   MGYPVGYTELLLPRIFLHLLSLLGLIRTLIDTGFRILGLPDFLESDPVSSSSSWLEPPYM 60

Query: 61  QFPLNP--------PPSAVILREFLPVVKLSDLSDP-----PENCVVCFYEFDGAEEIRL 120
               +         P +A +  E LPV++ S+L+ P      + C VC +EF+  +EIR 
Sbjct: 61  STAAHHHQESSFFFPVAARLAGEILPVIRFSELTRPGFGSGSDCCAVCLHEFENDDEIRR 120

Query: 121 LMNCKHIFHRRCVERW-INHNHDTCPLCRTPFVPDGMVDELNQRL 144
           L NC+HIFHR C++RW + +N  TCPLCRTPF+ D +    NQR+
Sbjct: 121 LTNCQHIFHRSCLDRWMMGYNQMTCPLCRTPFISDELQVAFNQRV 165

[Close]

BLAST of Carg05621 vs. TAIR 10
Match: AT4G00305.1 (RING/U-box superfamily protein )

HSP 1 Score: 92.0 bits (227), Expect = 4.6e-19
Identity = 36/70 (51.43%), Postives = 49/70 (70.00%), Query Frame = 0


Query: 67  EFLPVVKLSDLS---DPPENCVVCFYEFDGAEEIRLLMNCKHIFHRRCVERWINHNHDTC 126
           + LP  K  D+S   +PPE+C +C  EFDG +E+R L NC H++H+ C++RWI  +  TC
Sbjct: 51  DMLPATKFEDISSRVNPPESCRICQDEFDGGDEVRCLRNCVHVYHKTCIDRWIQDDKMTC 110

Query: 127 PLCRTPFVPD 134
           PLCRTP VPD
Sbjct: 111 PLCRTPIVPD 120

[Close]

BLAST of Carg05621 vs. TAIR 10
Match: AT4G11360.1 (RING-H2 finger A1B )

HSP 1 Score: 89.7 bits (221), Expect = 2.3e-18
Identity = 50/135 (37.04%), Postives = 70/135 (51.85%), Query Frame = 0


Query: 1   MGFPVSYTEELFPSILLLLLTVHDVLTHLVVSCFRLLWLPQFLRRESTPPEIQFPLNPPP 60
           MG P  + E   P  +L  L V      +V +    + LP FL    T       L    
Sbjct: 1   MGLPTDFKELQIPGYVLKTLYVIGFFRDMVDALCPYIGLPSFLDHNETSRSDPTRLALST 60

Query: 61  SAVILREFLPVVKLSD-LSDPPENCVVCFYEFDGAEEIRLLMNCKHIFHRRCVERWI-NH 120
           SA +  E +PVV+ SD L+DP + C VC  +F   ++IR L  C H+FH RC++RWI + 
Sbjct: 61  SATLANELIPVVRFSDLLTDPEDCCTVCLSDFVSDDKIRQLPKCGHVFHHRCLDRWIVDC 120

Query: 121 NHDTCPLCRTPFVPD 134
           N  TCP+CR  F+P+
Sbjct: 121 NKITCPICRNRFLPE 135

The following BLAST results are available for this feature:

BLAST of Carg05621 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7013755.1	6.7e-89	100.00	E3 ubiquitin-protein ligase RHA1B, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6575192.1	3.3e-88	99.38	Brassinosteroid-responsive RING protein 1, partial [Cucurbita argyrosperma subsp...	[more]
XP_022958975.1	4.1e-86	97.52	E3 ubiquitin-protein ligase RHA1B-like [Cucurbita moschata]	[more]
XP_023006675.1	5.5e-83	93.17	E3 ubiquitin-protein ligase RHA1B-like [Cucurbita maxima]	[more]
XP_023549219.1	3.5e-82	94.44	E3 ubiquitin-protein ligase RHA1B-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg05621 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XF92	1.2e-37	49.37	Brassinosteroid-responsive RING protein 1 OS=Arabidopsis thaliana OX=3702 GN=BRH...	[more]
Q9SUS5	3.2e-17	37.04	E3 ubiquitin-protein ligase RHA1B OS=Arabidopsis thaliana OX=3702 GN=RHA1B PE=2 ...	[more]
Q9SUS4	7.8e-16	36.50	Probable E3 ubiquitin-protein ligase RHA1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q5EAE9	3.2e-09	41.67	RING-H2 finger protein ATL43 OS=Arabidopsis thaliana OX=3702 GN=ATL43 PE=2 SV=2	[more]
Q9ZU51	3.2e-09	44.00	E3 ubiquitin-protein ligase RHA2B OS=Arabidopsis thaliana OX=3702 GN=RHA2B PE=1 ...	[more]

BLAST of Carg05621 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H500	2.0e-86	97.52	E3 ubiquitin-protein ligase RHA1B-like OS=Cucurbita moschata OX=3662 GN=LOC11146...	[more]
A0A6J1L0T4	2.7e-83	93.17	E3 ubiquitin-protein ligase RHA1B-like OS=Cucurbita maxima OX=3661 GN=LOC1114993...	[more]
A0A5D3BV35	1.5e-57	67.90	E3 ubiquitin-protein ligase RHA1B OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A1S3C782	1.5e-57	67.90	E3 ubiquitin-protein ligase RHA1B OS=Cucumis melo OX=3656 GN=LOC103497696 PE=4 S...	[more]
A0A0A0K874	1.6e-56	67.90	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G006705 P...	[more]

BLAST of Carg05621 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G61460.1	8.8e-39	49.37	brassinosteroid-responsive RING-H2	[more]
AT1G63840.1	4.0e-31	45.39	RING/U-box superfamily protein	[more]
AT5G41400.1	1.7e-26	39.39	RING/U-box superfamily protein	[more]
AT4G00305.1	4.6e-19	51.43	RING/U-box superfamily protein	[more]
AT4G11360.1	2.3e-18	37.04	RING-H2 finger A1B	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 84..126 e-value: 6.8E-5 score: 32.3
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 83..127 e-value: 4.5E-12 score: 46.1
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 84..127 score: 12.592549
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 67..133 e-value: 1.0E-16 score: 61.9
None	No IPR available	PANTHER	PTHR45969:SF10	BRH1 RING FINGER PROTEIN	coord: 1..151
None	No IPR available	PANTHER	PTHR45969	RING ZINC FINGER PROTEIN-RELATED	coord: 1..151
None	No IPR available	CDD	cd16448	RING-H2	coord: 84..127 e-value: 5.03602E-12 score: 55.5359
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 70..133

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg05621-RA	Carg05621-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0061630	ubiquitin protein ligase activity