Carg05613 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg05613
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Cysteine synthase
Location	Carg_Chr17: 2701144 .. 2705257 (+)
RNA-Seq Expression	Carg05613
Synteny	Carg05613

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGTACCATTTTAATGTTAAAAAAAAAAAACGAGTAGAATAATAATAAATAAATAAGGTAATGCAGAGGCAGCAAATGAGAGCCAAAGGAAGGATATCGAGTACAAAAGGCGGAAGAAGCCATTGGCGACCATCACTCCCAATTTCTTCCTCTAATTTCATCTCCTCTCTGTAATTTACCATCTCTCTAGGGCTCTACTTCTTCAAATTTCTCAAAGAAGCAGATTCCAGTAATCGACATCAATGGCGAGTGCCGCGTCGAGGAGTTTGATGAAGCAGAGGGCGGCGATTTCATCCGGCGCTTGTTCGTCGATCAGGAAACTATTCTCCACTCAAACGGTTCAAGATTCTTCTTCTTTTGCTCAGAGACTCAGAGATCTGCCGAAGGATCTTCCTGGAACAAGCATCAGACGAGATGCTGCTCATGTGAGTTTCTCGATCCCTTCCGATTCCGATTCTTGTCTTTCTTTGAACTGCTCCATACGCTTCCAATGAACTTCGATTATGCTTTCTCCATTTCTTTGTTCTTCAGTTCTGGATTTTTCTGATACCAATCAGGAACGAGAAGATATGGATAAAAAAATAATTTTCTTAAGAAAATAAAATAATTGCTGTGAAATTTTTAGGGTTGCTCAGAAAGAGCGTTGTAACTAATTCGTGTACAGTATAATTATTTATTTTATTTTTATTTTTTTAAAATACATAAAAAAAAAATAGTCATTAAAATTCGATAATTGTAAAAAAGAGTCTTATTAACTATATTTTAAAAGGGATTCTATAAAACATAATAATAAGCTAAATTAATTTAAATACTTTGTTTAAAAATAGAGTATTCCAAGATATTTTTTTAATTATTTTTTAAAAGTTTAATGATATTTTAAATTTTTGAAAGTTTAAAATTAATTTTTCAAATAAAATATAAAATTAAGAAAATATTTATAATTAATTTAGCGCATATATAGGAAGCTGGAGACTAAAAATAATTATTTTTGAAAAATAAAATAAAATATTTTGGAAATTACATTTAAAAATCCAAAAATACCTTTTAATTGGAAAATACAAAGACCTAATAATAATAATAATAATAATAATTAATAAAACTTGAAATTAATTGCCAAAAGGATTTATTTGTTCTTATTTGTTTTTATTTATTTATTTTTTTGGGATGGGAAAAAGTAAGGAATTTATTTTTTATGATGAGGACACGAATCACTAGAGAAATCTTTATTTTATTTTATTTTTAATATTTAATTGTTGGCTTCTGGTTTCAGTGTTTCCAGGGATGTTGTTTTTTAAAAATTAAATTAAATATTTTAAAAATACATAAATTAAATTTAACCAAAATCAAATAAGAAAATATATGTTTAATAACACAACATAATGCTCTTTTCTTTTTTTTTTTTTTTTTTTTGAAAAATTGTTGGATTTGTCTAAAAATAAAATTTAGTCAATTAATTTCGAAATTTATTATATTTTCTACGTAATCCCACTATTATATGCTTCATGTTTAATTAATTTATGCCCATAAATTAATTAATAATATTGAAACAAGGGAGTTGGAGAAAGAAAAAGAGTAATTTAAAAAAAAAAAAAAAATTAAAAGATTAATGTTTATATTATTTTCAGCTGATTGGGAAAACTCCAATTGTATATCTTAACAAAGTCACTGAAGGATGTGGAGGTTATGTAGCTGTGAAGCAAGAAATGATGCAGCCTACTTCCAGCATCAAAGACAGGTACTCCCACTCTCTTCTTACAATTCATTCTGTTCTCATTCATTTTGTACATTTTGTAATCTGTTATGTTTCCTTTCATCATCATTAGACCGGCTTTCGCGATGATCGCCGATGCCGAAAAGAAAGGTTTGATTACTCCTGGAAAGGTCAGCTTGTAAAACTCTTAGGCTGCAAGATTTAACAACAAAAATCTTCTTTTCTGAACTTCTGAAACTCGGGTTTTCGATGTCTAGACGACTTTGATCGAGCCAACGTCGGGAAACATGGGAATTAGTATGGCCTTTATGGCTACCATGAAAGGCTACAAAATGGTTCTGACCATGCCTTCTTATACAAGCTTGGAAAGAAGAGTTACAATGAGAGCATTTGGAGCTGAATTGATTGTCACCGACCCTACTAAAGGCATGGGAGGTACTGTCAAAAAGGCTTATGATCTTTTGGAATCAACACCTGATGGCTTCATGCTCCAACAGTTCTCAAACCCTGCCAATACTCAGGTTCCTAATTAAAATCCATTTTTCTTAGCTAATTTCTATGAAGTTTTTCGAAAACAAGATACCTCGCTTGATCGAAAGACATCGTTGGTAGATATATGATTGCCCTGAAGATGGTTTTTTTAAAAAAATGATCAAACAGGTGCATTTTGATACGACTGGCCCTGAGATATGGGAGGACACAAATGGACAAGTTGACATTTTTGTGATGGGAATAGGCAGTGGAGGGACTGTCTCTGGAGTGGGACAGTATCTGAAATCTCGAAATCCCAATGTTAAGGTATGTGATTATGTTATGAACTTTTGCTTCGTTAGAACATGGATTAGAATTCCTTACCAATGGTTCATATTCTGTCTCTTTACTGAAGATTTATGGAGTGGAGCCTGCTGAAAGTAATGTACTGAATGGTGGTAAACCAGGTATGATTAGAATGGCCTGCTTGGATGTTTTAGTTTGGATAATGTATTGGACATGAAATAGGTTAAAAATAAGAAATTTGAGAGTGAAATATATCAAACTTGTTTGTGAAGAAACATCTTGACTAAAAACTCATAACAAAACGAGATATCATCTCACATTGGTTGGAGAGGAGAACGAAACATTCCTTATAAGGGTGTGAAAACCTTTCCCTAGCGGACGCGTTTTAAAACTTTGAGGGGAAGCTCGTAAAGAAAAGCTCAAATAGGACAATATCTATTAGCGGTGTGCTTGGGCTATTACAAATGGTATCAGAGCTAGACACTGGGCGGTGTGCCAACGAGGACACTGGATCCCAAGGAGGGTGGATTGTGAGATCCCACATTGATTGGACAAGAGAACGAAACATTCGTTATAAGAGTGTGGAAACTTTTTCCTAATAGACGCGTTTTAAAACCTTGAGGGGAAGCCCAAGGGAAAGTCCAAAGAGAACAATATCTACTAGTGGTGGGCTTGGATGGCTACAGATGGTCAAGTCTCTCCATTTTTTCTAAGATGGGATTCTCAAGCGTGCATGTTCTAAAACTTTTGAAAACGACATGTCAGTGGGTTTTTCCGTGTTGTGTCCGTGCTTCGTTTGTGATAGGTTAATAGCAGATTCTTGATAGCTCTCATCAATCTTCTTTGATTGCTACCTCAGTGGTTACACGAAACTAATGGGGCCTGGTGTTATGAATTGGATTAGGTCCTCATCATATCACAGGCAATGGGGTTGGATTCAAGCCAGATATATTGGATATGGATGTAATGGATAAAGTTCTTGAAGTAAGTTTTTACCTTCACATATTTTTTACCTCCTAAAGATTAGATTACAAATTTACATATTCTCAAACAAATTTACTTTCCAGGTAAGCAGTGAAGATGCTGTAAATATGGCTAGGGAACTGGCTGTGAAGGAAGGACTAATGGTAAAAGAACAAACACCAACTTTTTTTTTACCTTTTATCTTACAGAATCATCTTCCTGTCCCTTTTGGTTTAAGATTTGAGCTTCATTTTTGTTGTTTGTTCAGGTCGGGATATCGTCCGGGGCCAATACGGTCGCTGCGCTCAGGCTTGCGAGATTACCCGAAAACAAAGGCAAACTTATTGTGGTAAGAACAGTTCTACACTCTTAAACAATCTGTAGAGTGTTTCTGGAGTCGAGATAAGTTGGAATAAATTGTTAATTTGGTTGCGTTTTCAGACAATTCATCCAAGTTTTGGGGAGAGATATTTGTCATCTGTGCTGTTCCAAGAATTGAGGAAAGAAGCTGAGAACATGCAACCCGTCTCAGTTGACTAAAGCTCGAGATACAGCTGAAGCTGAATCTACTCGTTTCCATTGTGAAGAATAAAATTTTATCCAATCTTATGTTCCATCAATGTTAGTTCATTTTGTATTGTTTAAAATTTTATATATATTGTAA

mRNA sequence

AGTACCATTTTAATGTTAAAAAAAAAAAACGAGTAGAATAATAATAAATAAATAAGGTAATGCAGAGGCAGCAAATGAGAGCCAAAGGAAGGATATCGAGTACAAAAGGCGGAAGAAGCCATTGGCGACCATCACTCCCAATTTCTTCCTCTAATTTCATCTCCTCTCTGTAATTTACCATCTCTCTAGGGCTCTACTTCTTCAAATTTCTCAAAGAAGCAGATTCCAGTAATCGACATCAATGGCGAGTGCCGCGTCGAGGAGTTTGATGAAGCAGAGGGCGGCGATTTCATCCGGCGCTTGTTCGTCGATCAGGAAACTATTCTCCACTCAAACGGTTCAAGATTCTTCTTCTTTTGCTCAGAGACTCAGAGATCTGCCGAAGGATCTTCCTGGAACAAGCATCAGACGAGATGCTGCTCATCTGATTGGGAAAACTCCAATTGTATATCTTAACAAAGTCACTGAAGGATGTGGAGGTTATGTAGCTGTGAAGCAAGAAATGATGCAGCCTACTTCCAGCATCAAAGACAGACCGGCTTTCGCGATGATCGCCGATGCCGAAAAGAAAGGTTTGATTACTCCTGGAAAGACGACTTTGATCGAGCCAACGTCGGGAAACATGGGAATTAGTATGGCCTTTATGGCTACCATGAAAGGCTACAAAATGGTTCTGACCATGCCTTCTTATACAAGCTTGGAAAGAAGAGTTACAATGAGAGCATTTGGAGCTGAATTGATTGTCACCGACCCTACTAAAGGCATGGGAGGTACTGTCAAAAAGGCTTATGATCTTTTGGAATCAACACCTGATGGCTTCATGCTCCAACAGTTCTCAAACCCTGCCAATACTCAGATATATGATTGCCCTGAAGATGGTTTTTTTAAAAAAATGATCAAACAGGTGCATTTTGATACGACTGGCCCTGAGATATGGGAGGACACAAATGGACAAGTTGACATTTTTGTGATGGGAATAGGCAGTGGAGGGACTGTCTCTGGAGTGGGACAGTATCTGAAATCTCGAAATCCCAATGTTAAGATTTATGGAGTGGAGCCTGCTGAAAGTAATGTACTGAATGGTGGTAAACCAGGTCCTCATCATATCACAGGCAATGGGGTTGGATTCAAGCCAGATATATTGGATATGGATGTAATGGATAAAGTTCTTGAAGTAAGCAGTGAAGATGCTGTAAATATGGCTAGGGAACTGGCTGTGAAGGAAGGACTAATGGTCGGGATATCGTCCGGGGCCAATACGGTCGCTGCGCTCAGGCTTGCGAGATTACCCGAAAACAAAGGCAAACTTATTGTGACAATTCATCCAAGTTTTGGGGAGAGATATTTGTCATCTGTGCTGTTCCAAGAATTGAGGAAAGAAGCTGAGAACATGCAACCCGTCTCAGTTGACTAAAGCTCGAGATACAGCTGAAGCTGAATCTACTCGTTTCCATTGTGAAGAATAAAATTTTATCCAATCTTATGTTCCATCAATGTTAGTTCATTTTGTATTGTTTAAAATTTTATATATATTGTAA

Coding sequence (CDS)

ATGGCGAGTGCCGCGTCGAGGAGTTTGATGAAGCAGAGGGCGGCGATTTCATCCGGCGCTTGTTCGTCGATCAGGAAACTATTCTCCACTCAAACGGTTCAAGATTCTTCTTCTTTTGCTCAGAGACTCAGAGATCTGCCGAAGGATCTTCCTGGAACAAGCATCAGACGAGATGCTGCTCATCTGATTGGGAAAACTCCAATTGTATATCTTAACAAAGTCACTGAAGGATGTGGAGGTTATGTAGCTGTGAAGCAAGAAATGATGCAGCCTACTTCCAGCATCAAAGACAGACCGGCTTTCGCGATGATCGCCGATGCCGAAAAGAAAGGTTTGATTACTCCTGGAAAGACGACTTTGATCGAGCCAACGTCGGGAAACATGGGAATTAGTATGGCCTTTATGGCTACCATGAAAGGCTACAAAATGGTTCTGACCATGCCTTCTTATACAAGCTTGGAAAGAAGAGTTACAATGAGAGCATTTGGAGCTGAATTGATTGTCACCGACCCTACTAAAGGCATGGGAGGTACTGTCAAAAAGGCTTATGATCTTTTGGAATCAACACCTGATGGCTTCATGCTCCAACAGTTCTCAAACCCTGCCAATACTCAGATATATGATTGCCCTGAAGATGGTTTTTTTAAAAAAATGATCAAACAGGTGCATTTTGATACGACTGGCCCTGAGATATGGGAGGACACAAATGGACAAGTTGACATTTTTGTGATGGGAATAGGCAGTGGAGGGACTGTCTCTGGAGTGGGACAGTATCTGAAATCTCGAAATCCCAATGTTAAGATTTATGGAGTGGAGCCTGCTGAAAGTAATGTACTGAATGGTGGTAAACCAGGTCCTCATCATATCACAGGCAATGGGGTTGGATTCAAGCCAGATATATTGGATATGGATGTAATGGATAAAGTTCTTGAAGTAAGCAGTGAAGATGCTGTAAATATGGCTAGGGAACTGGCTGTGAAGGAAGGACTAATGGTCGGGATATCGTCCGGGGCCAATACGGTCGCTGCGCTCAGGCTTGCGAGATTACCCGAAAACAAAGGCAAACTTATTGTGACAATTCATCCAAGTTTTGGGGAGAGATATTTGTCATCTGTGCTGTTCCAAGAATTGAGGAAAGAAGCTGAGAACATGCAACCCGTCTCAGTTGACTAA

Protein sequence

MASAASRSLMKQRAAISSGACSSIRKLFSTQTVQDSSSFAQRLRDLPKDLPGTSIRRDAAHLIGKTPIVYLNKVTEGCGGYVAVKQEMMQPTSSIKDRPAFAMIADAEKKGLITPGKTTLIEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGAELIVTDPTKGMGGTVKKAYDLLESTPDGFMLQQFSNPANTQIYDCPEDGFFKKMIKQVHFDTTGPEIWEDTNGQVDIFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDILDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTIHPSFGERYLSSVLFQELRKEAENMQPVSVD

Homology

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BLAST of Carg05613 vs. NCBI nr
Match: KAG7013747.1 (L-3-cyanoalanine synthase 1, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 765.4 bits (1975), Expect = 2.4e-217
Identity = 390/390 (100.00%), Postives = 390/390 (100.00%), Query Frame = 0


Query: 1   MASAASRSLMKQRAAISSGACSSIRKLFSTQTVQDSSSFAQRLRDLPKDLPGTSIRRDAA 60
           MASAASRSLMKQRAAISSGACSSIRKLFSTQTVQDSSSFAQRLRDLPKDLPGTSIRRDAA
Sbjct: 1   MASAASRSLMKQRAAISSGACSSIRKLFSTQTVQDSSSFAQRLRDLPKDLPGTSIRRDAA 60

Query: 61  HLIGKTPIVYLNKVTEGCGGYVAVKQEMMQPTSSIKDRPAFAMIADAEKKGLITPGKTTL 120
           HLIGKTPIVYLNKVTEGCGGYVAVKQEMMQPTSSIKDRPAFAMIADAEKKGLITPGKTTL
Sbjct: 61  HLIGKTPIVYLNKVTEGCGGYVAVKQEMMQPTSSIKDRPAFAMIADAEKKGLITPGKTTL 120

Query: 121 IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGAELIVTDPTKGMGGTVK 180
           IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGAELIVTDPTKGMGGTVK
Sbjct: 121 IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGAELIVTDPTKGMGGTVK 180

Query: 181 KAYDLLESTPDGFMLQQFSNPANTQIYDCPEDGFFKKMIKQVHFDTTGPEIWEDTNGQVD 240
           KAYDLLESTPDGFMLQQFSNPANTQIYDCPEDGFFKKMIKQVHFDTTGPEIWEDTNGQVD
Sbjct: 181 KAYDLLESTPDGFMLQQFSNPANTQIYDCPEDGFFKKMIKQVHFDTTGPEIWEDTNGQVD 240

Query: 241 IFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDI 300
           IFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDI
Sbjct: 241 IFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDI 300

Query: 301 LDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTI 360
           LDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTI
Sbjct: 301 LDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTI 360

Query: 361 HPSFGERYLSSVLFQELRKEAENMQPVSVD 391
           HPSFGERYLSSVLFQELRKEAENMQPVSVD
Sbjct: 361 HPSFGERYLSSVLFQELRKEAENMQPVSVD 390

[Close]

BLAST of Carg05613 vs. NCBI nr
Match: XP_022959417.1 (bifunctional L-3-cyanoalanine synthase/cysteine synthase 1, mitochondrial [Cucurbita moschata] >KAG6575182.1 L-3-cyanoalanine synthase 1, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 719.2 bits (1855), Expect = 2.0e-203
Identity = 374/390 (95.90%), Postives = 374/390 (95.90%), Query Frame = 0


Query: 1   MASAASRSLMKQRAAISSGACSSIRKLFSTQTVQDSSSFAQRLRDLPKDLPGTSIRRDAA 60
           MASAASRSLMKQRAAISSGACSSIRKLFSTQTVQDSSSFAQRLRDLPKDLPGTSIRRDAA
Sbjct: 1   MASAASRSLMKQRAAISSGACSSIRKLFSTQTVQDSSSFAQRLRDLPKDLPGTSIRRDAA 60

Query: 61  HLIGKTPIVYLNKVTEGCGGYVAVKQEMMQPTSSIKDRPAFAMIADAEKKGLITPGKTTL 120
           HLIGKTPIVYLNKVTEGCGGYVAVKQEMMQPTSSIKDRPAFAMIADAEKKGLITPGKTTL
Sbjct: 61  HLIGKTPIVYLNKVTEGCGGYVAVKQEMMQPTSSIKDRPAFAMIADAEKKGLITPGKTTL 120

Query: 121 IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGAELIVTDPTKGMGGTVK 180
           IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGAELIVTDPTKGMGGTVK
Sbjct: 121 IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGAELIVTDPTKGMGGTVK 180

Query: 181 KAYDLLESTPDGFMLQQFSNPANTQIYDCPEDGFFKKMIKQVHFDTTGPEIWEDTNGQVD 240
           KAYDLLESTPDGFMLQQFSNPANT                QVHFDTTGPEIWEDTNGQVD
Sbjct: 181 KAYDLLESTPDGFMLQQFSNPANT----------------QVHFDTTGPEIWEDTNGQVD 240

Query: 241 IFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDI 300
           IFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDI
Sbjct: 241 IFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDI 300

Query: 301 LDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTI 360
           LDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTI
Sbjct: 301 LDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTI 360

Query: 361 HPSFGERYLSSVLFQELRKEAENMQPVSVD 391
           HPSFGERYLSSVLFQELRKEAENMQPVSVD
Sbjct: 361 HPSFGERYLSSVLFQELRKEAENMQPVSVD 374

[Close]

BLAST of Carg05613 vs. NCBI nr
Match: XP_023006594.1 (bifunctional L-3-cyanoalanine synthase/cysteine synthase 1, mitochondrial [Cucurbita maxima])

HSP 1 Score: 718.0 bits (1852), Expect = 4.4e-203
Identity = 373/390 (95.64%), Postives = 374/390 (95.90%), Query Frame = 0


Query: 1   MASAASRSLMKQRAAISSGACSSIRKLFSTQTVQDSSSFAQRLRDLPKDLPGTSIRRDAA 60
           MASAASRSLMKQRAAISSGACSS+RKLFSTQTVQDSSSFAQRLRDLPKDLPGTSIRRDAA
Sbjct: 1   MASAASRSLMKQRAAISSGACSSMRKLFSTQTVQDSSSFAQRLRDLPKDLPGTSIRRDAA 60

Query: 61  HLIGKTPIVYLNKVTEGCGGYVAVKQEMMQPTSSIKDRPAFAMIADAEKKGLITPGKTTL 120
           HLIGKTPIVYLNKVTEGCGGYVAVKQEMMQPTSSIKDRPAFAMIADAEKKGLITPGKTTL
Sbjct: 61  HLIGKTPIVYLNKVTEGCGGYVAVKQEMMQPTSSIKDRPAFAMIADAEKKGLITPGKTTL 120

Query: 121 IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGAELIVTDPTKGMGGTVK 180
           IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGAELIVTDPTKGMGGTVK
Sbjct: 121 IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGAELIVTDPTKGMGGTVK 180

Query: 181 KAYDLLESTPDGFMLQQFSNPANTQIYDCPEDGFFKKMIKQVHFDTTGPEIWEDTNGQVD 240
           KAYDLLESTPDGFMLQQFSNPANT                QVHFDTTGPEIWEDTNGQVD
Sbjct: 181 KAYDLLESTPDGFMLQQFSNPANT----------------QVHFDTTGPEIWEDTNGQVD 240

Query: 241 IFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDI 300
           IFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDI
Sbjct: 241 IFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDI 300

Query: 301 LDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTI 360
           LDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTI
Sbjct: 301 LDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTI 360

Query: 361 HPSFGERYLSSVLFQELRKEAENMQPVSVD 391
           HPSFGERYLSSVLFQELRKEAENMQPVSVD
Sbjct: 361 HPSFGERYLSSVLFQELRKEAENMQPVSVD 374

[Close]

BLAST of Carg05613 vs. NCBI nr
Match: XP_023549039.1 (bifunctional L-3-cyanoalanine synthase/cysteine synthase 1, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 716.5 bits (1848), Expect = 1.3e-202
Identity = 372/390 (95.38%), Postives = 374/390 (95.90%), Query Frame = 0


Query: 1   MASAASRSLMKQRAAISSGACSSIRKLFSTQTVQDSSSFAQRLRDLPKDLPGTSIRRDAA 60
           MASAASRSLMKQRAAISSGACSS+RKLFSTQTVQDSSSFAQRLRDLPKDLPGTSIRRDAA
Sbjct: 1   MASAASRSLMKQRAAISSGACSSMRKLFSTQTVQDSSSFAQRLRDLPKDLPGTSIRRDAA 60

Query: 61  HLIGKTPIVYLNKVTEGCGGYVAVKQEMMQPTSSIKDRPAFAMIADAEKKGLITPGKTTL 120
           HLIGKTPIVYLNKV+EGCGGYVAVKQEMMQPTSSIKDRPAFAMIADAEKKGLITPGKTTL
Sbjct: 61  HLIGKTPIVYLNKVSEGCGGYVAVKQEMMQPTSSIKDRPAFAMIADAEKKGLITPGKTTL 120

Query: 121 IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGAELIVTDPTKGMGGTVK 180
           IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGAELIVTDPTKGMGGTVK
Sbjct: 121 IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGAELIVTDPTKGMGGTVK 180

Query: 181 KAYDLLESTPDGFMLQQFSNPANTQIYDCPEDGFFKKMIKQVHFDTTGPEIWEDTNGQVD 240
           KAYDLLESTPDGFMLQQFSNPANT                QVHFDTTGPEIWEDTNGQVD
Sbjct: 181 KAYDLLESTPDGFMLQQFSNPANT----------------QVHFDTTGPEIWEDTNGQVD 240

Query: 241 IFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDI 300
           IFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDI
Sbjct: 241 IFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDI 300

Query: 301 LDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTI 360
           LDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTI
Sbjct: 301 LDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTI 360

Query: 361 HPSFGERYLSSVLFQELRKEAENMQPVSVD 391
           HPSFGERYLSSVLFQELRKEAENMQPVSVD
Sbjct: 361 HPSFGERYLSSVLFQELRKEAENMQPVSVD 374

[Close]

BLAST of Carg05613 vs. NCBI nr
Match: XP_038875773.1 (bifunctional L-3-cyanoalanine synthase/cysteine synthase 1, mitochondrial [Benincasa hispida])

HSP 1 Score: 684.9 bits (1766), Expect = 4.1e-193
Identity = 352/390 (90.26%), Postives = 366/390 (93.85%), Query Frame = 0


Query: 1   MASAASRSLMKQRAAISSGACSSIRKLFSTQTVQDSSSFAQRLRDLPKDLPGTSIRRDAA 60
           MA+AA RSL+KQ+AAISS  CS++RKLFSTQTV +SSSF QRLRDLPKDLPGTSIRRDAA
Sbjct: 1   MAAAALRSLVKQKAAISSDGCSTVRKLFSTQTVLESSSFVQRLRDLPKDLPGTSIRRDAA 60

Query: 61  HLIGKTPIVYLNKVTEGCGGYVAVKQEMMQPTSSIKDRPAFAMIADAEKKGLITPGKTTL 120
           HLIGKTP+VYLNKVTEGCG Y+AVKQEMMQPTSSIKDRPAFAMI DAEKKGLITPGKTTL
Sbjct: 61  HLIGKTPMVYLNKVTEGCGAYIAVKQEMMQPTSSIKDRPAFAMINDAEKKGLITPGKTTL 120

Query: 121 IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGAELIVTDPTKGMGGTVK 180
           IEPTSGNMGISMAFMAT+KGYKMVLTMPSYTSLERRVTMRAFGAELI+TDPTKGMGGTVK
Sbjct: 121 IEPTSGNMGISMAFMATLKGYKMVLTMPSYTSLERRVTMRAFGAELILTDPTKGMGGTVK 180

Query: 181 KAYDLLESTPDGFMLQQFSNPANTQIYDCPEDGFFKKMIKQVHFDTTGPEIWEDTNGQVD 240
           KAYDLLESTP+GFMLQQFSNPANT                QVHF+TTGPEIWEDTNGQVD
Sbjct: 181 KAYDLLESTPNGFMLQQFSNPANT----------------QVHFETTGPEIWEDTNGQVD 240

Query: 241 IFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDI 300
           IFVMGIGSGGTVSGVGQYLKS+NPNVKIYGVEPAESNVLNGGKPGPH ITGNGVGFKPDI
Sbjct: 241 IFVMGIGSGGTVSGVGQYLKSKNPNVKIYGVEPAESNVLNGGKPGPHQITGNGVGFKPDI 300

Query: 301 LDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTI 360
           LDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANT+AALRLARLPENKGKLIVTI
Sbjct: 301 LDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANTIAALRLARLPENKGKLIVTI 360

Query: 361 HPSFGERYLSSVLFQELRKEAENMQPVSVD 391
           HPSFGERYLSSVLFQELRKEAENMQPVSVD
Sbjct: 361 HPSFGERYLSSVLFQELRKEAENMQPVSVD 374

[Close]

BLAST of Carg05613 vs. ExPASy Swiss-Prot
Match: Q1KLZ2 (L-3-cyanoalanine synthase 1, mitochondrial OS=Malus domestica OX=3750 GN=CAS1 PE=1 SV=1)

HSP 1 Score: 591.7 bits (1524), Expect = 6.2e-168
Identity = 301/390 (77.18%), Postives = 340/390 (87.18%), Query Frame = 0


Query: 4   AASRSLMKQRAAISSGACSSIRKLFSTQTVQ---DSSSFAQRLRDLPKDLPGTSIRRDAA 63
           AA RS +K+R+++        RKLFST+  Q   DS SFAQR+R+LPKDLPGT I+   +
Sbjct: 2   AALRSFLKKRSSVLCNGAMR-RKLFSTEVSQPLTDSPSFAQRIRNLPKDLPGTQIKTQVS 61

Query: 64  HLIGKTPIVYLNKVTEGCGGYVAVKQEMMQPTSSIKDRPAFAMIADAEKKGLITPGKTTL 123
            LIG+TPIVYLNKVTEGCG Y+AVKQEM QPT+SIKDRPA +MI DAE+KGLITPG+T L
Sbjct: 62  QLIGRTPIVYLNKVTEGCGAYIAVKQEMFQPTASIKDRPALSMINDAEEKGLITPGETIL 121

Query: 124 IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGAELIVTDPTKGMGGTVK 183
           IEPTSGNMGISMAFMA M+GYKMVLTMPSYTSLERRV MR FGA+LI+TDPTKGMGGTVK
Sbjct: 122 IEPTSGNMGISMAFMAAMRGYKMVLTMPSYTSLERRVCMRCFGADLILTDPTKGMGGTVK 181

Query: 184 KAYDLLESTPDGFMLQQFSNPANTQIYDCPEDGFFKKMIKQVHFDTTGPEIWEDTNGQVD 243
           KAYDLLESTP+ +MLQQFSNPANT                +VHF+TTGPEIWEDTNGQVD
Sbjct: 182 KAYDLLESTPNAYMLQQFSNPANT----------------KVHFETTGPEIWEDTNGQVD 241

Query: 244 IFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDI 303
           IFVMGIGSGGTVSGVGQYLKS+NPNV+IYGVEPAESNVLNGGKPGPH I GNGVGFKPDI
Sbjct: 242 IFVMGIGSGGTVSGVGQYLKSKNPNVQIYGVEPAESNVLNGGKPGPHSIMGNGVGFKPDI 301

Query: 304 LDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTI 363
           LD+D++++V+EV+SEDAVNMAR+LA+KEGLMVGISSGANTVAA+ LA+ PENKGKLIVT+
Sbjct: 302 LDLDMLERVIEVTSEDAVNMARQLALKEGLMVGISSGANTVAAMELAKKPENKGKLIVTV 361

Query: 364 HPSFGERYLSSVLFQELRKEAENMQPVSVD 391
           HPSFGERYLSSVLFQELR+EAENMQPV+VD
Sbjct: 362 HPSFGERYLSSVLFQELRQEAENMQPVAVD 374

[Close]

BLAST of Carg05613 vs. ExPASy Swiss-Prot
Match: Q76MX2 (Bifunctional L-3-cyanoalanine synthase/cysteine synthase 1, mitochondrial OS=Solanum tuberosum OX=4113 GN=PCAS-1 PE=1 SV=1)

HSP 1 Score: 591.7 bits (1524), Expect = 6.2e-168
Identity = 296/366 (80.87%), Postives = 325/366 (88.80%), Query Frame = 0


Query: 25  RKLFSTQTVQDSSSFAQRLRDLPKDLPGTSIRRDAAHLIGKTPIVYLNKVTEGCGGYVAV 84
           R+ +S++     SSFAQRLRDLPK LPGT+I+   + LIGKTP+VYLNKV+EGCG Y+AV
Sbjct: 7   RRFYSSE-----SSFAQRLRDLPKYLPGTNIKTQVSQLIGKTPLVYLNKVSEGCGAYIAV 66

Query: 85  KQEMMQPTSSIKDRPAFAMIADAEKKGLITPGKTTLIEPTSGNMGISMAFMATMKGYKMV 144
           KQEMMQPTSSIKDRPAFAMI DAEKKGLITPGKTTLIEPTSGNMGISMAFMA MKGYKM+
Sbjct: 67  KQEMMQPTSSIKDRPAFAMINDAEKKGLITPGKTTLIEPTSGNMGISMAFMAAMKGYKMI 126

Query: 145 LTMPSYTSLERRVTMRAFGAELIVTDPTKGMGGTVKKAYDLLESTPDGFMLQQFSNPANT 204
           LTMPSYTSLERRVTMRAFGA+L+ TDPTKGMGGT+KKAYDLLESTP+ +MLQQFSNPANT
Sbjct: 127 LTMPSYTSLERRVTMRAFGADLVTTDPTKGMGGTIKKAYDLLESTPNAYMLQQFSNPANT 186

Query: 205 QIYDCPEDGFFKKMIKQVHFDTTGPEIWEDTNGQVDIFVMGIGSGGTVSGVGQYLKSRNP 264
                           Q HF+TTGPEIWEDT G VDIFVMGIGSGGTVSGVGQYLKS+NP
Sbjct: 187 ----------------QAHFETTGPEIWEDTQGNVDIFVMGIGSGGTVSGVGQYLKSKNP 246

Query: 265 NVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDILDMDVMDKVLEVSSEDAVNMAREL 324
           NVKIYG+EP ESNVLNGG PGPH ITGNGVGFKPDILDMDVM++VL VSSE++VNMAREL
Sbjct: 247 NVKIYGIEPTESNVLNGGNPGPHEITGNGVGFKPDILDMDVMEEVLMVSSEESVNMAREL 306

Query: 325 AVKEGLMVGISSGANTVAALRLARLPENKGKLIVTIHPSFGERYLSSVLFQELRKEAENM 384
           A+KEGLMVGISSGANTVAALRLA  PENKGKLIVTIHPSFGERYLSSVL++++RKEA+NM
Sbjct: 307 ALKEGLMVGISSGANTVAALRLANRPENKGKLIVTIHPSFGERYLSSVLYEDIRKEAQNM 351

Query: 385 QPVSVD 391
           QPVSVD
Sbjct: 367 QPVSVD 351

[Close]

BLAST of Carg05613 vs. ExPASy Swiss-Prot
Match: Q1KLZ1 (L-3-cyanoalanine synthase 2, mitochondrial OS=Malus domestica OX=3750 GN=CAS2 PE=1 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 6.9e-167
Identity = 300/390 (76.92%), Postives = 337/390 (86.41%), Query Frame = 0


Query: 4   AASRSLMKQRAAISSGACSSIRKLFSTQTVQ---DSSSFAQRLRDLPKDLPGTSIRRDAA 63
           AA RS +K+R+++     +   +LFS Q  Q   DS SFAQR+ +LPKDLP T I+   +
Sbjct: 2   AALRSFLKKRSSVLCNGGAVRHRLFSAQVSQPIPDSPSFAQRIGNLPKDLPATQIKTQVS 61

Query: 64  HLIGKTPIVYLNKVTEGCGGYVAVKQEMMQPTSSIKDRPAFAMIADAEKKGLITPGKTTL 123
            LIG+TPIVYLNKVTEGCG ++AVKQEM QPT+SIKDRPA +MI DAE+KGLITPG+TTL
Sbjct: 62  QLIGRTPIVYLNKVTEGCGAFIAVKQEMFQPTASIKDRPALSMINDAEEKGLITPGETTL 121

Query: 124 IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGAELIVTDPTKGMGGTVK 183
           IEPTSGNMGISMAFMA MKGYKMVLTMPSYTSLERRV MR FGA+LI+TDPTKGMGGTVK
Sbjct: 122 IEPTSGNMGISMAFMAAMKGYKMVLTMPSYTSLERRVCMRCFGADLILTDPTKGMGGTVK 181

Query: 184 KAYDLLESTPDGFMLQQFSNPANTQIYDCPEDGFFKKMIKQVHFDTTGPEIWEDTNGQVD 243
           KAYDLLESTP+ FMLQQFSNPANT                +VHF+TTGPEIWEDTNGQVD
Sbjct: 182 KAYDLLESTPNAFMLQQFSNPANT----------------KVHFETTGPEIWEDTNGQVD 241

Query: 244 IFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDI 303
           IFVMGIGSGGTVSGVGQYLKS+NPNV+IYGVEPAESNVLNGGKPGPH ITGNGVGFKPDI
Sbjct: 242 IFVMGIGSGGTVSGVGQYLKSKNPNVQIYGVEPAESNVLNGGKPGPHSITGNGVGFKPDI 301

Query: 304 LDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTI 363
           LDMD+M++V+EV SEDAVNMAR+LA+KEGLMVGISSGANTVAA+ LA+ PENKGKLIVT+
Sbjct: 302 LDMDMMERVIEVRSEDAVNMARQLALKEGLMVGISSGANTVAAMELAKKPENKGKLIVTV 361

Query: 364 HPSFGERYLSSVLFQELRKEAENMQPVSVD 391
           H SFGERYLSSVLFQ+LR+EAENMQPV+VD
Sbjct: 362 HASFGERYLSSVLFQDLRQEAENMQPVAVD 375

[Close]

BLAST of Carg05613 vs. ExPASy Swiss-Prot
Match: Q9S757 (Bifunctional L-3-cyanoalanine synthase/cysteine synthase C1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=CYSC1 PE=1 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 4.6e-163
Identity = 299/390 (76.67%), Postives = 330/390 (84.62%), Query Frame = 0


Query: 1   MASAASRSLMKQRAAISSGACSSIRKLFSTQTVQDSSSFAQRLRDLPKDLPGTSIRRDAA 60
           MAS + R L ++     S    ++RKLFST     S SFAQRLRDLPKD P T+ +RDA+
Sbjct: 1   MASVSRRLLRRETIPCFS---HTVRKLFST---VGSPSFAQRLRDLPKDFPSTNAKRDAS 60

Query: 61  HLIGKTPIVYLNKVTEGCGGYVAVKQEMMQPTSSIKDRPAFAMIADAEKKGLITPGKTTL 120
            LIGKTP+V+LNKVTEGC  YVA KQE  QPT SIKDRPA AMIADAEKK LI PGKTTL
Sbjct: 61  LLIGKTPLVFLNKVTEGCEAYVAAKQEHFQPTCSIKDRPAIAMIADAEKKKLIIPGKTTL 120

Query: 121 IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGAELIVTDPTKGMGGTVK 180
           IEPTSGNMGIS+AFMA MKGY++++TMPSYTSLERRVTMR+FGAEL++TDP KGMGGTVK
Sbjct: 121 IEPTSGNMGISLAFMAAMKGYRIIMTMPSYTSLERRVTMRSFGAELVLTDPAKGMGGTVK 180

Query: 181 KAYDLLESTPDGFMLQQFSNPANTQIYDCPEDGFFKKMIKQVHFDTTGPEIWEDTNGQVD 240
           KAYDLL+STPD FM QQF+NPANTQI                HFDTTGPEIWEDT G VD
Sbjct: 181 KAYDLLDSTPDAFMCQQFANPANTQI----------------HFDTTGPEIWEDTLGNVD 240

Query: 241 IFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDI 300
           IFVMGIGSGGTVSGVG+YLKS+NPNVKIYGVEPAESN+LNGGKPGPH ITGNGVGFKP+I
Sbjct: 241 IFVMGIGSGGTVSGVGRYLKSKNPNVKIYGVEPAESNILNGGKPGPHAITGNGVGFKPEI 300

Query: 301 LDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTI 360
           LDMDVM+ VLEVSSEDA+ MARELA+KEGLMVGISSGANTVAA+RLA++PENKGKLIVTI
Sbjct: 301 LDMDVMESVLEVSSEDAIKMARELALKEGLMVGISSGANTVAAIRLAKMPENKGKLIVTI 360

Query: 361 HPSFGERYLSSVLFQELRKEAENMQPVSVD 391
           H SFGERYLSSVLF ELRKEAE M+PVSVD
Sbjct: 361 HASFGERYLSSVLFDELRKEAEEMKPVSVD 368

[Close]

BLAST of Carg05613 vs. ExPASy Swiss-Prot
Match: Q9FS29 (Bifunctional L-3-cyanoalanine synthase/cysteine synthase 2, mitochondrial OS=Solanum tuberosum OX=4113 GN=PCAS-2 PE=1 SV=1)

HSP 1 Score: 557.0 bits (1434), Expect = 1.7e-157
Identity = 280/341 (82.11%), Postives = 305/341 (89.44%), Query Frame = 0


Query: 50  LPGTSIRRDAAHLIGKTPIVYLNKVTEGCGGYVAVKQEMMQPTSSIKDRPAFAMIADAEK 109
           LP T+I+ + + LIGKTP+VYL KVTEGCG Y+AVKQEM QPTSSIKDRPA AMI DAEK
Sbjct: 23  LPHTNIKSEVSQLIGKTPMVYLKKVTEGCGAYIAVKQEMFQPTSSIKDRPALAMINDAEK 82

Query: 110 KGLITPGKTTLIEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGAELIVT 169
           KGLI+P KTTLIEPTSGNMGISMAFMA MKGYKMVLTMPSYTS+ERRVTMRAFGA+LI+T
Sbjct: 83  KGLISPEKTTLIEPTSGNMGISMAFMAAMKGYKMVLTMPSYTSMERRVTMRAFGADLILT 142

Query: 170 DPTKGMGGTVKKAYDLLESTPDGFMLQQFSNPANTQIYDCPEDGFFKKMIKQVHFDTTGP 229
           DPTKGMGGTVKKAY+LLESTP+ FMLQQFSNPANT                QVHFDTTGP
Sbjct: 143 DPTKGMGGTVKKAYELLESTPNAFMLQQFSNPANT----------------QVHFDTTGP 202

Query: 230 EIWEDTNGQVDIFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHI 289
           EIWE+T G VDIFVMGIGSGGTV+GVG YLKS+NPNVKIYG+EP ESN+LNGGKPGPHHI
Sbjct: 203 EIWEETLGNVDIFVMGIGSGGTVTGVGLYLKSKNPNVKIYGLEPTESNILNGGKPGPHHI 262

Query: 290 TGNGVGFKPDILDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLARL 349
           TGNGVG KPDI+DMD+M++VL VSSEDAVNMARELAVKEGLMVGISSGANTVAALRLA+ 
Sbjct: 263 TGNGVGSKPDIVDMDLMEEVLMVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLAQK 322

Query: 350 PENKGKLIVTIHPSFGERYLSSVLFQELRKEAENMQPVSVD 391
           PENKGKLIVT+H SFGERYLSSVL+Q+LRKEAENMQPVSVD
Sbjct: 323 PENKGKLIVTVHASFGERYLSSVLYQDLRKEAENMQPVSVD 347

[Close]

BLAST of Carg05613 vs. ExPASy TrEMBL
Match: A0A6J1H4G6 (Cysteine synthase OS=Cucurbita moschata OX=3662 GN=LOC111460401 PE=3 SV=1)

HSP 1 Score: 719.2 bits (1855), Expect = 9.6e-204
Identity = 374/390 (95.90%), Postives = 374/390 (95.90%), Query Frame = 0


Query: 1   MASAASRSLMKQRAAISSGACSSIRKLFSTQTVQDSSSFAQRLRDLPKDLPGTSIRRDAA 60
           MASAASRSLMKQRAAISSGACSSIRKLFSTQTVQDSSSFAQRLRDLPKDLPGTSIRRDAA
Sbjct: 1   MASAASRSLMKQRAAISSGACSSIRKLFSTQTVQDSSSFAQRLRDLPKDLPGTSIRRDAA 60

Query: 61  HLIGKTPIVYLNKVTEGCGGYVAVKQEMMQPTSSIKDRPAFAMIADAEKKGLITPGKTTL 120
           HLIGKTPIVYLNKVTEGCGGYVAVKQEMMQPTSSIKDRPAFAMIADAEKKGLITPGKTTL
Sbjct: 61  HLIGKTPIVYLNKVTEGCGGYVAVKQEMMQPTSSIKDRPAFAMIADAEKKGLITPGKTTL 120

Query: 121 IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGAELIVTDPTKGMGGTVK 180
           IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGAELIVTDPTKGMGGTVK
Sbjct: 121 IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGAELIVTDPTKGMGGTVK 180

Query: 181 KAYDLLESTPDGFMLQQFSNPANTQIYDCPEDGFFKKMIKQVHFDTTGPEIWEDTNGQVD 240
           KAYDLLESTPDGFMLQQFSNPANT                QVHFDTTGPEIWEDTNGQVD
Sbjct: 181 KAYDLLESTPDGFMLQQFSNPANT----------------QVHFDTTGPEIWEDTNGQVD 240

Query: 241 IFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDI 300
           IFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDI
Sbjct: 241 IFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDI 300

Query: 301 LDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTI 360
           LDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTI
Sbjct: 301 LDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTI 360

Query: 361 HPSFGERYLSSVLFQELRKEAENMQPVSVD 391
           HPSFGERYLSSVLFQELRKEAENMQPVSVD
Sbjct: 361 HPSFGERYLSSVLFQELRKEAENMQPVSVD 374

[Close]

BLAST of Carg05613 vs. ExPASy TrEMBL
Match: A0A6J1L2L7 (Cysteine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499275 PE=3 SV=1)

HSP 1 Score: 718.0 bits (1852), Expect = 2.1e-203
Identity = 373/390 (95.64%), Postives = 374/390 (95.90%), Query Frame = 0


Query: 1   MASAASRSLMKQRAAISSGACSSIRKLFSTQTVQDSSSFAQRLRDLPKDLPGTSIRRDAA 60
           MASAASRSLMKQRAAISSGACSS+RKLFSTQTVQDSSSFAQRLRDLPKDLPGTSIRRDAA
Sbjct: 1   MASAASRSLMKQRAAISSGACSSMRKLFSTQTVQDSSSFAQRLRDLPKDLPGTSIRRDAA 60

Query: 61  HLIGKTPIVYLNKVTEGCGGYVAVKQEMMQPTSSIKDRPAFAMIADAEKKGLITPGKTTL 120
           HLIGKTPIVYLNKVTEGCGGYVAVKQEMMQPTSSIKDRPAFAMIADAEKKGLITPGKTTL
Sbjct: 61  HLIGKTPIVYLNKVTEGCGGYVAVKQEMMQPTSSIKDRPAFAMIADAEKKGLITPGKTTL 120

Query: 121 IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGAELIVTDPTKGMGGTVK 180
           IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGAELIVTDPTKGMGGTVK
Sbjct: 121 IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGAELIVTDPTKGMGGTVK 180

Query: 181 KAYDLLESTPDGFMLQQFSNPANTQIYDCPEDGFFKKMIKQVHFDTTGPEIWEDTNGQVD 240
           KAYDLLESTPDGFMLQQFSNPANT                QVHFDTTGPEIWEDTNGQVD
Sbjct: 181 KAYDLLESTPDGFMLQQFSNPANT----------------QVHFDTTGPEIWEDTNGQVD 240

Query: 241 IFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDI 300
           IFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDI
Sbjct: 241 IFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDI 300

Query: 301 LDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTI 360
           LDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTI
Sbjct: 301 LDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTI 360

Query: 361 HPSFGERYLSSVLFQELRKEAENMQPVSVD 391
           HPSFGERYLSSVLFQELRKEAENMQPVSVD
Sbjct: 361 HPSFGERYLSSVLFQELRKEAENMQPVSVD 374

[Close]

BLAST of Carg05613 vs. ExPASy TrEMBL
Match: A0A6J1DTT8 (Cysteine synthase OS=Momordica charantia OX=3673 GN=LOC111024353 PE=3 SV=1)

HSP 1 Score: 680.6 bits (1755), Expect = 3.8e-192
Identity = 355/390 (91.03%), Postives = 365/390 (93.59%), Query Frame = 0


Query: 1   MASAASRSLMKQRAAISSGACSSIRKLFSTQTVQDSSSFAQRLRDLPKDLPGTSIRRDAA 60
           MA+AASR L+KQRAAI SG+ S++RKLFSTQTV DS SFAQRLRDLPKDLPGTSIRRDAA
Sbjct: 1   MAAAASRILLKQRAAIWSGS-STVRKLFSTQTVLDSPSFAQRLRDLPKDLPGTSIRRDAA 60

Query: 61  HLIGKTPIVYLNKVTEGCGGYVAVKQEMMQPTSSIKDRPAFAMIADAEKKGLITPGKTTL 120
           HLIGKTPIVYLNKVTE CG YVAVKQE+MQPTSSIKDRPAFAMI DAEKKGLITPGKTTL
Sbjct: 61  HLIGKTPIVYLNKVTEDCGAYVAVKQEIMQPTSSIKDRPAFAMITDAEKKGLITPGKTTL 120

Query: 121 IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGAELIVTDPTKGMGGTVK 180
           IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGA+LI+TDPTKGMGGTVK
Sbjct: 121 IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGADLILTDPTKGMGGTVK 180

Query: 181 KAYDLLESTPDGFMLQQFSNPANTQIYDCPEDGFFKKMIKQVHFDTTGPEIWEDTNGQVD 240
           KAYDLLESTP+GFMLQQFSNPANT                QVHF+TTGPEIWEDTNGQVD
Sbjct: 181 KAYDLLESTPNGFMLQQFSNPANT----------------QVHFETTGPEIWEDTNGQVD 240

Query: 241 IFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDI 300
           IFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDI
Sbjct: 241 IFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDI 300

Query: 301 LDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTI 360
           LDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTI
Sbjct: 301 LDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTI 360

Query: 361 HPSFGERYLSSVLFQELRKEAENMQPVSVD 391
           HPSFGERYLSSVLFQELR EAENMQPVSVD
Sbjct: 361 HPSFGERYLSSVLFQELRNEAENMQPVSVD 373

[Close]

BLAST of Carg05613 vs. ExPASy TrEMBL
Match: A0A0A0K9Z6 (Cysteine synthase OS=Cucumis sativus OX=3659 GN=Csa_6G004600 PE=3 SV=1)

HSP 1 Score: 679.1 bits (1751), Expect = 1.1e-191
Identity = 348/391 (89.00%), Postives = 365/391 (93.35%), Query Frame = 0


Query: 1   MASAASRSLMKQRAAISSGACSSIRKLFSTQTV-QDSSSFAQRLRDLPKDLPGTSIRRDA 60
           MA+AA R+L+KQ+A ISS  CS++RK FSTQTV + SSSFAQRLRDLPKDLPGTS+RRDA
Sbjct: 1   MAAAALRTLVKQKAGISSDGCSTVRKFFSTQTVFESSSSFAQRLRDLPKDLPGTSVRRDA 60

Query: 61  AHLIGKTPIVYLNKVTEGCGGYVAVKQEMMQPTSSIKDRPAFAMIADAEKKGLITPGKTT 120
           AHLIGKTP+VYLNKVTEGCG Y+AVKQEMMQPTSSIKDRPAFAMI DAEKKGLITPGKTT
Sbjct: 61  AHLIGKTPLVYLNKVTEGCGAYIAVKQEMMQPTSSIKDRPAFAMINDAEKKGLITPGKTT 120

Query: 121 LIEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGAELIVTDPTKGMGGTV 180
           LIEPTSGNMGISMAFMAT+KGYKMVLTMPSYTSLERRVTMRAFGAELI+TDPTKGMGGTV
Sbjct: 121 LIEPTSGNMGISMAFMATLKGYKMVLTMPSYTSLERRVTMRAFGAELILTDPTKGMGGTV 180

Query: 181 KKAYDLLESTPDGFMLQQFSNPANTQIYDCPEDGFFKKMIKQVHFDTTGPEIWEDTNGQV 240
           KKAYDLLESTPDGFMLQQF+NPANT                QVHF+TTGPEIWEDTNGQV
Sbjct: 181 KKAYDLLESTPDGFMLQQFANPANT----------------QVHFETTGPEIWEDTNGQV 240

Query: 241 DIFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPD 300
           DIFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPD
Sbjct: 241 DIFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPD 300

Query: 301 ILDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVT 360
           ILDMDVMDKVLEVSS+DAVNMARELAVKEGLMVGISSGANTVAALRLA+LPENKGKLIVT
Sbjct: 301 ILDMDVMDKVLEVSSDDAVNMARELAVKEGLMVGISSGANTVAALRLAKLPENKGKLIVT 360

Query: 361 IHPSFGERYLSSVLFQELRKEAENMQPVSVD 391
           +HPSFGERYLSSVLFQELR EAENMQPVSVD
Sbjct: 361 VHPSFGERYLSSVLFQELRNEAENMQPVSVD 375

[Close]

BLAST of Carg05613 vs. ExPASy TrEMBL
Match: A0A5D3BW04 (Cysteine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G001170 PE=3 SV=1)

HSP 1 Score: 674.5 bits (1739), Expect = 2.7e-190
Identity = 350/395 (88.61%), Postives = 366/395 (92.66%), Query Frame = 0


Query: 1   MASAASRSLMKQRAAISSGACS-SIRKLFSTQTVQD----SSSFAQRLRDLPKDLPGTSI 60
           MA+AA RSL+KQ+A ISS  CS ++RKLFSTQTV D    SSSFAQRLRDLPKDLPGTS+
Sbjct: 1   MAAAALRSLVKQKAGISSDGCSTTVRKLFSTQTVLDSSSSSSSFAQRLRDLPKDLPGTSV 60

Query: 61  RRDAAHLIGKTPIVYLNKVTEGCGGYVAVKQEMMQPTSSIKDRPAFAMIADAEKKGLITP 120
           RRDAAHLIGKTP+VYLNKVTEGCG Y+AVKQEMMQPTSSIKDRPAFAMI DAEKKGLITP
Sbjct: 61  RRDAAHLIGKTPLVYLNKVTEGCGAYIAVKQEMMQPTSSIKDRPAFAMINDAEKKGLITP 120

Query: 121 GKTTLIEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGAELIVTDPTKGM 180
           G+TTLIEPTSGNMGISMAFMAT+KGYKMVLTMPSYTSLERRVTMRAFGAELI+TDPTKGM
Sbjct: 121 GQTTLIEPTSGNMGISMAFMATLKGYKMVLTMPSYTSLERRVTMRAFGAELILTDPTKGM 180

Query: 181 GGTVKKAYDLLESTPDGFMLQQFSNPANTQIYDCPEDGFFKKMIKQVHFDTTGPEIWEDT 240
           GGTVKKAYDLLESTP+GFMLQQFSNPANT                QVHF+TTGPEIWEDT
Sbjct: 181 GGTVKKAYDLLESTPNGFMLQQFSNPANT----------------QVHFETTGPEIWEDT 240

Query: 241 NGQVDIFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVG 300
           NG VDIFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESN+LNGGKPGPHHITGNGVG
Sbjct: 241 NGLVDIFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNILNGGKPGPHHITGNGVG 300

Query: 301 FKPDILDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGK 360
           FKPDILDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLA+LPENKGK
Sbjct: 301 FKPDILDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLAKLPENKGK 360

Query: 361 LIVTIHPSFGERYLSSVLFQELRKEAENMQPVSVD 391
           LIVT+HPSFGERYLSSVLFQELRKEAENMQPVSVD
Sbjct: 361 LIVTVHPSFGERYLSSVLFQELRKEAENMQPVSVD 379

[Close]

BLAST of Carg05613 vs. TAIR 10
Match: AT3G61440.1 (cysteine synthase C1 )

HSP 1 Score: 575.5 bits (1482), Expect = 3.3e-164
Identity = 299/390 (76.67%), Postives = 330/390 (84.62%), Query Frame = 0


Query: 1   MASAASRSLMKQRAAISSGACSSIRKLFSTQTVQDSSSFAQRLRDLPKDLPGTSIRRDAA 60
           MAS + R L ++     S    ++RKLFST     S SFAQRLRDLPKD P T+ +RDA+
Sbjct: 1   MASVSRRLLRRETIPCFS---HTVRKLFST---VGSPSFAQRLRDLPKDFPSTNAKRDAS 60

Query: 61  HLIGKTPIVYLNKVTEGCGGYVAVKQEMMQPTSSIKDRPAFAMIADAEKKGLITPGKTTL 120
            LIGKTP+V+LNKVTEGC  YVA KQE  QPT SIKDRPA AMIADAEKK LI PGKTTL
Sbjct: 61  LLIGKTPLVFLNKVTEGCEAYVAAKQEHFQPTCSIKDRPAIAMIADAEKKKLIIPGKTTL 120

Query: 121 IEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGAELIVTDPTKGMGGTVK 180
           IEPTSGNMGIS+AFMA MKGY++++TMPSYTSLERRVTMR+FGAEL++TDP KGMGGTVK
Sbjct: 121 IEPTSGNMGISLAFMAAMKGYRIIMTMPSYTSLERRVTMRSFGAELVLTDPAKGMGGTVK 180

Query: 181 KAYDLLESTPDGFMLQQFSNPANTQIYDCPEDGFFKKMIKQVHFDTTGPEIWEDTNGQVD 240
           KAYDLL+STPD FM QQF+NPANTQI                HFDTTGPEIWEDT G VD
Sbjct: 181 KAYDLLDSTPDAFMCQQFANPANTQI----------------HFDTTGPEIWEDTLGNVD 240

Query: 241 IFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDI 300
           IFVMGIGSGGTVSGVG+YLKS+NPNVKIYGVEPAESN+LNGGKPGPH ITGNGVGFKP+I
Sbjct: 241 IFVMGIGSGGTVSGVGRYLKSKNPNVKIYGVEPAESNILNGGKPGPHAITGNGVGFKPEI 300

Query: 301 LDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTI 360
           LDMDVM+ VLEVSSEDA+ MARELA+KEGLMVGISSGANTVAA+RLA++PENKGKLIVTI
Sbjct: 301 LDMDVMESVLEVSSEDAIKMARELALKEGLMVGISSGANTVAAIRLAKMPENKGKLIVTI 360

Query: 361 HPSFGERYLSSVLFQELRKEAENMQPVSVD 391
           H SFGERYLSSVLF ELRKEAE M+PVSVD
Sbjct: 361 HASFGERYLSSVLFDELRKEAEEMKPVSVD 368

[Close]

BLAST of Carg05613 vs. TAIR 10
Match: AT3G61440.2 (cysteine synthase C1 )

HSP 1 Score: 473.4 bits (1217), Expect = 1.8e-133
Identity = 237/288 (82.29%), Postives = 258/288 (89.58%), Query Frame = 0


Query: 103 MIADAEKKGLITPGKTTLIEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAF 162
           MIADAEKK LI PGKTTLIEPTSGNMGIS+AFMA MKGY++++TMPSYTSLERRVTMR+F
Sbjct: 1   MIADAEKKKLIIPGKTTLIEPTSGNMGISLAFMAAMKGYRIIMTMPSYTSLERRVTMRSF 60

Query: 163 GAELIVTDPTKGMGGTVKKAYDLLESTPDGFMLQQFSNPANTQIYDCPEDGFFKKMIKQV 222
           GAEL++TDP KGMGGTVKKAYDLL+STPD FM QQF+NPANTQI                
Sbjct: 61  GAELVLTDPAKGMGGTVKKAYDLLDSTPDAFMCQQFANPANTQI---------------- 120

Query: 223 HFDTTGPEIWEDTNGQVDIFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGG 282
           HFDTTGPEIWEDT G VDIFVMGIGSGGTVSGVG+YLKS+NPNVKIYGVEPAESN+LNGG
Sbjct: 121 HFDTTGPEIWEDTLGNVDIFVMGIGSGGTVSGVGRYLKSKNPNVKIYGVEPAESNILNGG 180

Query: 283 KPGPHHITGNGVGFKPDILDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANTVA 342
           KPGPH ITGNGVGFKP+ILDMDVM+ VLEVSSEDA+ MARELA+KEGLMVGISSGANTVA
Sbjct: 181 KPGPHAITGNGVGFKPEILDMDVMESVLEVSSEDAIKMARELALKEGLMVGISSGANTVA 240

Query: 343 ALRLARLPENKGKLIVTIHPSFGERYLSSVLFQELRKEAENMQPVSVD 391
           A+RLA++PENKGKLIVTIH SFGERYLSSVLF ELRKEAE M+PVSVD
Sbjct: 241 AIRLAKMPENKGKLIVTIHASFGERYLSSVLFDELRKEAEEMKPVSVD 272

[Close]

BLAST of Carg05613 vs. TAIR 10
Match: AT3G61440.3 (cysteine synthase C1 )

HSP 1 Score: 430.3 bits (1105), Expect = 1.7e-120
Identity = 214/263 (81.37%), Postives = 235/263 (89.35%), Query Frame = 0


Query: 128 MGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGAELIVTDPTKGMGGTVKKAYDLLE 187
           MGIS+AFMA MKGY++++TMPSYTSLERRVTMR+FGAEL++TDP KGMGGTVKKAYDLL+
Sbjct: 1   MGISLAFMAAMKGYRIIMTMPSYTSLERRVTMRSFGAELVLTDPAKGMGGTVKKAYDLLD 60

Query: 188 STPDGFMLQQFSNPANTQIYDCPEDGFFKKMIKQVHFDTTGPEIWEDTNGQVDIFVMGIG 247
           STPD FM QQF+NPANTQI                HFDTTGPEIWEDT G VDIFVMGIG
Sbjct: 61  STPDAFMCQQFANPANTQI----------------HFDTTGPEIWEDTLGNVDIFVMGIG 120

Query: 248 SGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGVGFKPDILDMDVMD 307
           SGGTVSGVG+YLKS+NPNVKIYGVEPAESN+LNGGKPGPH ITGNGVGFKP+ILDMDVM+
Sbjct: 121 SGGTVSGVGRYLKSKNPNVKIYGVEPAESNILNGGKPGPHAITGNGVGFKPEILDMDVME 180

Query: 308 KVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLARLPENKGKLIVTIHPSFGER 367
            VLEVSSEDA+ MARELA+KEGLMVGISSGANTVAA+RLA++PENKGKLIVTIH SFGER
Sbjct: 181 SVLEVSSEDAIKMARELALKEGLMVGISSGANTVAAIRLAKMPENKGKLIVTIHASFGER 240

Query: 368 YLSSVLFQELRKEAENMQPVSVD 391
           YLSSVLF ELRKEAE M+PVSVD
Sbjct: 241 YLSSVLFDELRKEAEEMKPVSVD 247

[Close]

BLAST of Carg05613 vs. TAIR 10
Match: AT4G14880.1 (O-acetylserine (thiol) lyase (OAS-TL) isoform A1 )

HSP 1 Score: 403.3 bits (1035), Expect = 2.2e-112
Identity = 194/330 (58.79%), Postives = 251/330 (76.06%), Query Frame = 0


Query: 55  IRRDAAHLIGKTPIVYLNKVTEGCGGYVAVKQEMMQPTSSIKDRPAFAMIADAEKKGLIT 114
           I +D   LIG TP+VYLN V EGC G VA K EMM+P SS+KDR  F+MI+DAEKKGLI 
Sbjct: 5   IAKDVTELIGNTPLVYLNNVAEGCVGRVAAKLEMMEPCSSVKDRIGFSMISDAEKKGLIK 64

Query: 115 PGKTTLIEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGAELIVTDPTKG 174
           PG++ LIEPTSGN G+ +AF A  KGYK+++TMP+  S ERR+ + AFG EL++TDP KG
Sbjct: 65  PGESVLIEPTSGNTGVGLAFTAAAKGYKLIITMPASMSTERRIILLAFGVELVLTDPAKG 124

Query: 175 MGGTVKKAYDLLESTPDGFMLQQFSNPANTQIYDCPEDGFFKKMIKQVHFDTTGPEIWED 234
           M G + KA ++L  TP+G+MLQQF NPAN +I                H++TTGPEIW+ 
Sbjct: 125 MKGAIAKAEEILAKTPNGYMLQQFENPANPKI----------------HYETTGPEIWKG 184

Query: 235 TNGQVDIFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGV 294
           T G++D FV GIG+GGT++G G+YLK +N NVK+YGVEP ES +L+GGKPGPH I G G 
Sbjct: 185 TGGKIDGFVSGIGTGGTITGAGKYLKEQNANVKLYGVEPVESAILSGGKPGPHKIQGIGA 244

Query: 295 GFKPDILDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLARLPENKG 354
           GF P +L++D++D+V++VSS+++++MAR+LA+KEGL+VGISSGA   AA++LA+ PEN G
Sbjct: 245 GFIPSVLNVDLIDEVVQVSSDESIDMARQLALKEGLLVGISSGAAAAAAIKLAQRPENAG 304

Query: 355 KLIVTIHPSFGERYLSSVLFQELRKEAENM 385
           KL V I PSFGERYLS+VLF   RKEAE M
Sbjct: 305 KLFVAIFPSFGERYLSTVLFDATRKEAEAM 318

[Close]

BLAST of Carg05613 vs. TAIR 10
Match: AT4G14880.2 (O-acetylserine (thiol) lyase (OAS-TL) isoform A1 )

HSP 1 Score: 403.3 bits (1035), Expect = 2.2e-112
Identity = 194/330 (58.79%), Postives = 251/330 (76.06%), Query Frame = 0


Query: 55  IRRDAAHLIGKTPIVYLNKVTEGCGGYVAVKQEMMQPTSSIKDRPAFAMIADAEKKGLIT 114
           I +D   LIG TP+VYLN V EGC G VA K EMM+P SS+KDR  F+MI+DAEKKGLI 
Sbjct: 5   IAKDVTELIGNTPLVYLNNVAEGCVGRVAAKLEMMEPCSSVKDRIGFSMISDAEKKGLIK 64

Query: 115 PGKTTLIEPTSGNMGISMAFMATMKGYKMVLTMPSYTSLERRVTMRAFGAELIVTDPTKG 174
           PG++ LIEPTSGN G+ +AF A  KGYK+++TMP+  S ERR+ + AFG EL++TDP KG
Sbjct: 65  PGESVLIEPTSGNTGVGLAFTAAAKGYKLIITMPASMSTERRIILLAFGVELVLTDPAKG 124

Query: 175 MGGTVKKAYDLLESTPDGFMLQQFSNPANTQIYDCPEDGFFKKMIKQVHFDTTGPEIWED 234
           M G + KA ++L  TP+G+MLQQF NPAN +I                H++TTGPEIW+ 
Sbjct: 125 MKGAIAKAEEILAKTPNGYMLQQFENPANPKI----------------HYETTGPEIWKG 184

Query: 235 TNGQVDIFVMGIGSGGTVSGVGQYLKSRNPNVKIYGVEPAESNVLNGGKPGPHHITGNGV 294
           T G++D FV GIG+GGT++G G+YLK +N NVK+YGVEP ES +L+GGKPGPH I G G 
Sbjct: 185 TGGKIDGFVSGIGTGGTITGAGKYLKEQNANVKLYGVEPVESAILSGGKPGPHKIQGIGA 244

Query: 295 GFKPDILDMDVMDKVLEVSSEDAVNMARELAVKEGLMVGISSGANTVAALRLARLPENKG 354
           GF P +L++D++D+V++VSS+++++MAR+LA+KEGL+VGISSGA   AA++LA+ PEN G
Sbjct: 245 GFIPSVLNVDLIDEVVQVSSDESIDMARQLALKEGLLVGISSGAAAAAAIKLAQRPENAG 304

Query: 355 KLIVTIHPSFGERYLSSVLFQELRKEAENM 385
           KL V I PSFGERYLS+VLF   RKEAE M
Sbjct: 305 KLFVAIFPSFGERYLSTVLFDATRKEAEAM 318

The following BLAST results are available for this feature:

BLAST of Carg05613 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7013747.1	2.4e-217	100.00	L-3-cyanoalanine synthase 1, mitochondrial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022959417.1	2.0e-203	95.90	bifunctional L-3-cyanoalanine synthase/cysteine synthase 1, mitochondrial [Cucur...	[more]
XP_023006594.1	4.4e-203	95.64	bifunctional L-3-cyanoalanine synthase/cysteine synthase 1, mitochondrial [Cucur...	[more]
XP_023549039.1	1.3e-202	95.38	bifunctional L-3-cyanoalanine synthase/cysteine synthase 1, mitochondrial [Cucur...	[more]
XP_038875773.1	4.1e-193	90.26	bifunctional L-3-cyanoalanine synthase/cysteine synthase 1, mitochondrial [Benin...	[more]

BLAST of Carg05613 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q1KLZ2	6.2e-168	77.18	L-3-cyanoalanine synthase 1, mitochondrial OS=Malus domestica OX=3750 GN=CAS1 PE...	[more]
Q76MX2	6.2e-168	80.87	Bifunctional L-3-cyanoalanine synthase/cysteine synthase 1, mitochondrial OS=Sol...	[more]
Q1KLZ1	6.9e-167	76.92	L-3-cyanoalanine synthase 2, mitochondrial OS=Malus domestica OX=3750 GN=CAS2 PE...	[more]
Q9S757	4.6e-163	76.67	Bifunctional L-3-cyanoalanine synthase/cysteine synthase C1, mitochondrial OS=Ar...	[more]
Q9FS29	1.7e-157	82.11	Bifunctional L-3-cyanoalanine synthase/cysteine synthase 2, mitochondrial OS=Sol...	[more]

BLAST of Carg05613 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H4G6	9.6e-204	95.90	Cysteine synthase OS=Cucurbita moschata OX=3662 GN=LOC111460401 PE=3 SV=1	[more]
A0A6J1L2L7	2.1e-203	95.64	Cysteine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499275 PE=3 SV=1	[more]
A0A6J1DTT8	3.8e-192	91.03	Cysteine synthase OS=Momordica charantia OX=3673 GN=LOC111024353 PE=3 SV=1	[more]
A0A0A0K9Z6	1.1e-191	89.00	Cysteine synthase OS=Cucumis sativus OX=3659 GN=Csa_6G004600 PE=3 SV=1	[more]
A0A5D3BW04	2.7e-190	88.61	Cysteine synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G001...	[more]

BLAST of Carg05613 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G61440.1	3.3e-164	76.67	cysteine synthase C1	[more]
AT3G61440.2	1.8e-133	82.29	cysteine synthase C1	[more]
AT3G61440.3	1.7e-120	81.37	cysteine synthase C1	[more]
AT4G14880.1	2.2e-112	58.79	O-acetylserine (thiol) lyase (OAS-TL) isoform A1	[more]
AT4G14880.2	2.2e-112	58.79	O-acetylserine (thiol) lyase (OAS-TL) isoform A1	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036052	Tryptophan synthase beta subunit-like PLP-dependent enzyme	GENE3D	3.40.50.1100		coord: 62..370 e-value: 1.2E-121 score: 407.3
IPR036052	Tryptophan synthase beta subunit-like PLP-dependent enzyme	GENE3D	3.40.50.1100		coord: 90..197 e-value: 1.2E-121 score: 407.3
IPR036052	Tryptophan synthase beta subunit-like PLP-dependent enzyme	SUPERFAMILY	53686	Tryptophan synthase beta subunit-like PLP-dependent enzymes	coord: 57..379
IPR005856	Cysteine synthase	TIGRFAM	TIGR01136	TIGR01136	coord: 61..374 e-value: 9.6E-116 score: 384.3
IPR001926	Pyridoxal-phosphate dependent enzyme	PFAM	PF00291	PALP	coord: 62..362 e-value: 6.9E-62 score: 209.5
IPR005859	Cysteine synthase CysK	TIGRFAM	TIGR01139	TIGR01139	coord: 61..374 e-value: 1.7E-117 score: 389.9
IPR031111	L-3-cyanoalanine synthase, mitochondrial	PANTHER	PTHR10314:SF80	BIFUNCTIONAL L-3-CYANOALANINE SYNTHASE/CYSTEINE SYNTHASE C1, MITOCHONDRIAL	coord: 16..389
None	No IPR available	PANTHER	PTHR10314	CYSTATHIONINE BETA-SYNTHASE	coord: 16..389
None	No IPR available	CDD	cd01561	CBS_like	coord: 64..370 e-value: 2.27128E-139 score: 396.884
IPR001216	Cysteine synthase/cystathionine beta-synthase, pyridoxal-phosphate attachment site	PROSITE	PS00901	CYS_SYNTHASE	coord: 85..103

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg05613-RA	Carg05613-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019499	cyanide metabolic process
biological_process	GO:0006535	cysteine biosynthetic process from serine
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0004124	cysteine synthase activity
molecular_function	GO:0050017	L-3-cyanoalanine synthase activity