Carg05548 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg05548
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionpectinesterase inhibitor-like
LocationCarg_Chr17: 2140300 .. 2140601 (+)
RNA-Seq ExpressionCarg05548
SyntenyCarg05548
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAGACACAACGCCCCAAAACAACACTGTATCGATCATCTGTCACGATGCCAATAACCCATCGTTATGCTAGCAATTGTTGAAACCAGTGAGAAACTTAGATGTCGTTGGCGTAACTAATTACACCTTCAACCTTACACATACGAGCACCATCGAATGTCTCCACTACGCACGGCGACTTGAGGGGGAAGAGACAATCAATCCAGAGTTTAAGAATCGATATTCAGATTGTTCGAAACACATAAACTCTTCGTTGCCCGACGTAGAAACTGCTCAATATTGCATGGGTTATCATACATAA

mRNA sequence

ATGCAGACACAACGCCCCAAAACAACACTGTATCGATCATCTGTCACGATGCCAATAACCCATCTGAGAAACTTAGATGTCGTTGGCGTAACTAATTACACCTTCAACCTTACACATACGAGCACCATCGAATGTCTCCACTACGCACGGCGACTTGAGGGGGAAGAGACAATCAATCCAGAGTTTAAGAATCGATATTCAGATTGTTCGAAACACATAAACTCTTCGTTGCCCGACGTAGAAACTGCTCAATATTGCATGGGTTATCATACATAA

Coding sequence (CDS)

ATGCAGACACAACGCCCCAAAACAACACTGTATCGATCATCTGTCACGATGCCAATAACCCATCTGAGAAACTTAGATGTCGTTGGCGTAACTAATTACACCTTCAACCTTACACATACGAGCACCATCGAATGTCTCCACTACGCACGGCGACTTGAGGGGGAAGAGACAATCAATCCAGAGTTTAAGAATCGATATTCAGATTGTTCGAAACACATAAACTCTTCGTTGCCCGACGTAGAAACTGCTCAATATTGCATGGGTTATCATACATAA

Protein sequence

MQTQRPKTTLYRSSVTMPITHLRNLDVVGVTNYTFNLTHTSTIECLHYARRLEGEETINPEFKNRYSDCSKHINSSLPDVETAQYCMGYHT
Homology
BLAST of Carg05548 vs. NCBI nr
Match: KAG7013683.1 (hypothetical protein SDJN02_23850, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 191.4 bits (485), Expect = 3.3e-45
Identity = 91/91 (100.00%), Postives = 91/91 (100.00%), Query Frame = 0

Query: 1  MQTQRPKTTLYRSSVTMPITHLRNLDVVGVTNYTFNLTHTSTIECLHYARRLEGEETINP 60
          MQTQRPKTTLYRSSVTMPITHLRNLDVVGVTNYTFNLTHTSTIECLHYARRLEGEETINP
Sbjct: 1  MQTQRPKTTLYRSSVTMPITHLRNLDVVGVTNYTFNLTHTSTIECLHYARRLEGEETINP 60

Query: 61 EFKNRYSDCSKHINSSLPDVETAQYCMGYHT 92
          EFKNRYSDCSKHINSSLPDVETAQYCMGYHT
Sbjct: 61 EFKNRYSDCSKHINSSLPDVETAQYCMGYHT 91

BLAST of Carg05548 vs. NCBI nr
Match: XP_022958860.1 (pectinesterase inhibitor-like [Cucurbita moschata])

HSP 1 Score: 89.0 bits (219), Expect = 2.3e-14
Identity = 42/68 (61.76%), Postives = 50/68 (73.53%), Query Frame = 0

Query: 22  LRNLDVVGVTNYTFNLTHTSTIECLHYARRLEGEETINPEFKNRYSDCSKHINSSLPDVE 81
           +RNLD V +TNYT N T TS IECLHYA+ LE +ET+N E K RYS CSK I S++ D+E
Sbjct: 53  VRNLDSVSLTNYTLNFTRTSAIECLHYAQGLEAKETVNLELKKRYSYCSKSIKSTVTDIE 112

Query: 82  TAQYCMGY 90
            AQ  MGY
Sbjct: 113 VAQEYMGY 120

BLAST of Carg05548 vs. NCBI nr
Match: XP_023548317.1 (pectinesterase inhibitor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 59.7 bits (143), Expect = 1.5e-05
Identity = 27/66 (40.91%), Postives = 41/66 (62.12%), Query Frame = 0

Query: 24  NLDVVGVTNYTFNLTHTSTIECLHYARRLEGEETINPEFKNRYSDCSKHINSSLPDVETA 83
           NL+   + NY  +L HT+ +EC    +RL+ EET N E K RYS+C + I+SS+  +E+ 
Sbjct: 55  NLNATNMANYMIDLAHTNAVECRRRVQRLKAEETTNLELKKRYSECWQCIDSSVSAIESG 114

Query: 84  QYCMGY 90
           +  M Y
Sbjct: 115 KQYMEY 120

BLAST of Carg05548 vs. NCBI nr
Match: XP_022958859.1 (pectinesterase inhibitor 1-like [Cucurbita moschata])

HSP 1 Score: 53.9 bits (128), Expect = 8.3e-04
Identity = 22/54 (40.74%), Postives = 35/54 (64.81%), Query Frame = 0

Query: 36 NLTHTSTIECLHYARRLEGEETINPEFKNRYSDCSKHINSSLPDVETAQYCMGY 90
          +L HTS +EC    ++LE EET+NP+ K RYS+C +  NSS+  +E  +  + +
Sbjct: 7  HLAHTSVVECRRRVQQLEAEETVNPKLKKRYSECLESTNSSVNAIEVGKQHLAF 60

BLAST of Carg05548 vs. ExPASy TrEMBL
Match: A0A6J1H4N5 (pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111460015 PE=4 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 1.1e-14
Identity = 42/68 (61.76%), Postives = 50/68 (73.53%), Query Frame = 0

Query: 22  LRNLDVVGVTNYTFNLTHTSTIECLHYARRLEGEETINPEFKNRYSDCSKHINSSLPDVE 81
           +RNLD V +TNYT N T TS IECLHYA+ LE +ET+N E K RYS CSK I S++ D+E
Sbjct: 53  VRNLDSVSLTNYTLNFTRTSAIECLHYAQGLEAKETVNLELKKRYSYCSKSIKSTVTDIE 112

Query: 82  TAQYCMGY 90
            AQ  MGY
Sbjct: 113 VAQEYMGY 120

BLAST of Carg05548 vs. ExPASy TrEMBL
Match: A0A6J1H6C1 (pectinesterase inhibitor 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460014 PE=4 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 4.0e-04
Identity = 22/54 (40.74%), Postives = 35/54 (64.81%), Query Frame = 0

Query: 36 NLTHTSTIECLHYARRLEGEETINPEFKNRYSDCSKHINSSLPDVETAQYCMGY 90
          +L HTS +EC    ++LE EET+NP+ K RYS+C +  NSS+  +E  +  + +
Sbjct: 7  HLAHTSVVECRRRVQQLEAEETVNPKLKKRYSECLESTNSSVNAIEVGKQHLAF 60

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7013683.13.3e-45100.00hypothetical protein SDJN02_23850, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_022958860.12.3e-1461.76pectinesterase inhibitor-like [Cucurbita moschata][more]
XP_023548317.11.5e-0540.91pectinesterase inhibitor-like [Cucurbita pepo subsp. pepo][more]
XP_022958859.18.3e-0440.74pectinesterase inhibitor 1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1H4N51.1e-1461.76pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111460015 PE=4... [more]
A0A6J1H6C14.0e-0440.74pectinesterase inhibitor 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460014 PE... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 22..87

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg05548-RACarg05548-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0004857 enzyme inhibitor activity