Carg05543 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg05543
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionouter envelope pore protein 16-2, chloroplastic-like
LocationCarg_Chr17: 2112320 .. 2114964 (+)
RNA-Seq ExpressionCarg05543
SyntenyCarg05543
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAGCGCATGCGATCGTTTCGTCTCACTGATTTTAGCATTCAAATCGACTGCAGATTTTCGGAGTTCAATCTCTGATTTATAGAAGCAGAAACGAGAAATGGGAGGTAAATGCTTTGATAACAGTGGCGGAGGCTTCTTGTACGACTTTGGCCATCCTCTTGTCAATCGCGTTGCTGATAGCTTCGTCAAGGCCGCTGGGGTTCGTTAGCTTTCATGATCTTAATTTCTGTTAACTTTTGTGATATTCTTGATGTGATATTGTTTTGTGTTGGATCTCAATGTCAATGTGGTAACCTTAACATGTCGATGGTTCGTTGTGCAGATAGGAGCATTGCAATCTGCATCTCGAGAGGCGTATGTTACGGTTGCGGGAAGTAAGATCCGTTTCATTTAGTATTAAGATCTGATCCTCGTGCTTTGATTTCACTCGATTTCTTTCTACATTTCAAATTTTCAACTCTTTGTTTTGATTTTGATTTATTTTCGTGTATCTATTCCTTTGCTTTATGTCACTCGTTTAGGAGACTTTTGAGGTTAAATTGGTTAACAAAAATTTGTAATTCTCGAAAGATTGTTATCGTCCGTACAGGTCTTGATTTGAACAGTGGAGCATCATCTGAATTATCCAATGGTAGAAACAAAAGTTTTACAGGTCTTACAGGTACCCGAATCTTCCTACGAACTCAGCTCTGTTTATCCACAATCAAAGTCGTTCTTGAGTCCGCCTACTGTGTTTAATTTTGTTTTTCTTGCCTGGTGAAAGTATGAACTATGTTTTAAATTGAATTCTAATAATTATATTGTTATAAATCGATACCATTTGTTTAGATGTGCGTTATTTTGGGCAATATATGGGACATGGATTGCCAAATTATTGCTTGATACAGACTAAAACTGAACTGCCCCTTATTATGTTCCTGAATAATTTGTGTGATGTCCAAGTTAATCCATCCTTCGAGATGCTTGAAGCTTATTTCTTCATATAATGTGCAGGTGCAACCAACAAAGAGTCTCTTGAAGCCATGGTGAGTGTTTGTAAAGGGTGATCAAGTTTTGAGTTTGTGATGAATTATTTACAGTGGTGGTCAATAAACATGTGATGATTTTTCATTTTATGGGTACATCAGGTGAAGAGCGTGGGAAAGGAATCTATGCAATGGGGTCAGTATCCCTTCTCTGAATTCTTGAATACTGAACATTCATGAATGTTAAATTGTACATCTGGTTTATGTTACTTCACCAATTAGATGGATAATGGGTTGAGTGCAATTTTTTGCTTTTGTGTCTATTTACTTCACCAATTGTAACTTTTACAAGATTCTTCTCTTGGAGGTTGCTTGGCAGATTATTTCCTCTTATGTCATGTTGTATTTCACCCTAAAAAGGGTACATCGAGAAAAGAATTACCAAGCTTTTGGTTTGGTGGTGTGATTTCATAGCATATTTATGCTGTTTAATTTTATTTTATTGCTCTCAGCTTTTTTTTTTCCCTTCTTTTTTGGGTTGTAGGATTAGCTGCTGGTGTTTATTCTGGTCTCACATACGGTTTAAAGGAGGCTCGTGGAGCACATGACTGGGTAAGCCTTCCACCATTTACAAATAAATGTTCATTCTTTGGATCCAAGTATGGGGTATGATCATTTAGAGCAGAGGCGGGCTAAAATTCTTCATGGGGAGGGATAGAACTCATCCTTTTCCTGAACGCCCGTCAATTAGCTAATCATAGACATATTTTAGTTAATATTTTAGTTAATGGTTCTATAGTGGATATAGCAAGTTATCGTTGCAAACCCCGAGACATTATTACAGCAAAGGATGAAAAAGAATTGAGAGCACTGATTAGTTATGTTGGTTCATGAACTTCAATCGAACATTCGTTTAAGACGATGTGGTTGGTTAGAGAGCAACGTGCGACTTGAAGGACATCATTTATCAAGGGAAGGATCCAATTGTGGTTGGTTAGAGAGCAACGTGCAACTTGAAGGACATCATTTATCAAGGGAAGGATCCAATTGACCAAGTTTCAAATTCAAACGTCAAATTTGATCAAAAGTGCATCACAAGAGAAATAGTTCGACGTTTAGTCCTTCTGGTCTCATGTCTTGAAATCTTGATCCATGATCCATTCTTTCATTCCATCCATTGCAGAAGAACAGTGCAGTTGCAGGTGCCATGACGGGCGTAGCTGTGGCGCTTACATCGGACAAGCCTTCTCACGAGCACATCGTACAATATGCCATCACTGGTGCTGCTGTATCGACCGCTGCAAATATTTTTGCTGGCATATTTTAGGTATGAGTGTGGTACCGGGGAGCCATTTGACAAATTGTGGGATGTTCATCCTTCATGTTCATTCCGTCTTTTATTATTAAGATGACTAATGATGTTCATCTTTTGGACTTTTGGTTTAATTATTTAGTTCCTTTTATCCGCTTCTGGTTGGGATAGAAAATGGTTTAACCAATAAATCTGGAAATTTGTATGATTTTAACCAATAACTGAAACCATTGGCCTTACTGGTGAAGGGTAAAATAGGCACAAGTTCATGAAAAATCTATTATTCAAAATATTGTGGCCATATTTAGAATATTTACTTATTTATCTTTATTTTTTTGGAAAAATATATTTTATTTATTTATCCTCT

mRNA sequence

GAAGCGCATGCGATCGTTTCGTCTCACTGATTTTAGCATTCAAATCGACTGCAGATTTTCGGAGTTCAATCTCTGATTTATAGAAGCAGAAACGAGAAATGGGAGGTAAATGCTTTGATAACAGTGGCGGAGGCTTCTTGTACGACTTTGGCCATCCTCTTGTCAATCGCGTTGCTGATAGCTTCGTCAAGGCCGCTGGGATAGGAGCATTGCAATCTGCATCTCGAGAGGCGTATGTTACGGTTGCGGGAAGTCTTGATTTGAACAGTGGAGCATCATCTGAATTATCCAATGGTAGAAACAAAAGTTTTACAGGTCTTACAGGTGCAACCAACAAAGAGTCTCTTGAAGCCATGGTGAAGAGCGTGGGAAAGGAATCTATGCAATGGGGATTAGCTGCTGGTGTTTATTCTGGTCTCACATACGGTTTAAAGGAGGCTCGTGGAGCACATGACTGGAAGAACAGTGCAGTTGCAGGTGCCATGACGGGCGTAGCTGTGGCGCTTACATCGGACAAGCCTTCTCACGAGCACATCGTACAATATGCCATCACTGGTGCTGCTGTATCGACCGCTGCAAATATTTTTGCTGGCATATTTTAGGTATGAGTGTGGTACCGGGGAGCCATTTGACAAATTGTGGGATGTTCATCCTTCATGTTCATTCCGTCTTTTATTATTAAGATGACTAATGATGTTCATCTTTTGGACTTTTGGTTTAATTATTTAGTTCCTTTTATCCGCTTCTGGTTGGGATAGAAAATGGTTTAACCAATAAATCTGGAAATTTGTATGATTTTAACCAATAACTGAAACCATTGGCCTTACTGGTGAAGGGTAAAATAGGCACAAGTTCATGAAAAATCTATTATTCAAAATATTGTGGCCATATTTAGAATATTTACTTATTTATCTTTATTTTTTTGGAAAAATATATTTTATTTATTTATCCTCT

Coding sequence (CDS)

ATGGGAGGTAAATGCTTTGATAACAGTGGCGGAGGCTTCTTGTACGACTTTGGCCATCCTCTTGTCAATCGCGTTGCTGATAGCTTCGTCAAGGCCGCTGGGATAGGAGCATTGCAATCTGCATCTCGAGAGGCGTATGTTACGGTTGCGGGAAGTCTTGATTTGAACAGTGGAGCATCATCTGAATTATCCAATGGTAGAAACAAAAGTTTTACAGGTCTTACAGGTGCAACCAACAAAGAGTCTCTTGAAGCCATGGTGAAGAGCGTGGGAAAGGAATCTATGCAATGGGGATTAGCTGCTGGTGTTTATTCTGGTCTCACATACGGTTTAAAGGAGGCTCGTGGAGCACATGACTGGAAGAACAGTGCAGTTGCAGGTGCCATGACGGGCGTAGCTGTGGCGCTTACATCGGACAAGCCTTCTCACGAGCACATCGTACAATATGCCATCACTGGTGCTGCTGTATCGACCGCTGCAAATATTTTTGCTGGCATATTTTAG

Protein sequence

MGGKCFDNSGGGFLYDFGHPLVNRVADSFVKAAGIGALQSASREAYVTVAGSLDLNSGASSELSNGRNKSFTGLTGATNKESLEAMVKSVGKESMQWGLAAGVYSGLTYGLKEARGAHDWKNSAVAGAMTGVAVALTSDKPSHEHIVQYAITGAAVSTAANIFAGIF
Homology
BLAST of Carg05543 vs. NCBI nr
Match: KAG6575109.1 (Outer envelope pore protein 16-2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013678.1 Outer envelope pore protein 16-2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 323.2 bits (827), Expect = 1.4e-84
Identity = 167/167 (100.00%), Postives = 167/167 (100.00%), Query Frame = 0

Query: 1   MGGKCFDNSGGGFLYDFGHPLVNRVADSFVKAAGIGALQSASREAYVTVAGSLDLNSGAS 60
           MGGKCFDNSGGGFLYDFGHPLVNRVADSFVKAAGIGALQSASREAYVTVAGSLDLNSGAS
Sbjct: 1   MGGKCFDNSGGGFLYDFGHPLVNRVADSFVKAAGIGALQSASREAYVTVAGSLDLNSGAS 60

Query: 61  SELSNGRNKSFTGLTGATNKESLEAMVKSVGKESMQWGLAAGVYSGLTYGLKEARGAHDW 120
           SELSNGRNKSFTGLTGATNKESLEAMVKSVGKESMQWGLAAGVYSGLTYGLKEARGAHDW
Sbjct: 61  SELSNGRNKSFTGLTGATNKESLEAMVKSVGKESMQWGLAAGVYSGLTYGLKEARGAHDW 120

Query: 121 KNSAVAGAMTGVAVALTSDKPSHEHIVQYAITGAAVSTAANIFAGIF 168
           KNSAVAGAMTGVAVALTSDKPSHEHIVQYAITGAAVSTAANIFAGIF
Sbjct: 121 KNSAVAGAMTGVAVALTSDKPSHEHIVQYAITGAAVSTAANIFAGIF 167

BLAST of Carg05543 vs. NCBI nr
Match: XP_022959516.1 (outer envelope pore protein 16-2, chloroplastic-like isoform X1 [Cucurbita moschata] >XP_022959517.1 outer envelope pore protein 16-2, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 317.4 bits (812), Expect = 7.4e-83
Identity = 163/167 (97.60%), Postives = 166/167 (99.40%), Query Frame = 0

Query: 1   MGGKCFDNSGGGFLYDFGHPLVNRVADSFVKAAGIGALQSASREAYVTVAGSLDLNSGAS 60
           MGGKCF+NSGGGFLYDFGHPLVNRVADSFVKAAGIGALQS SREAYVTVAGSLDLNSGAS
Sbjct: 1   MGGKCFENSGGGFLYDFGHPLVNRVADSFVKAAGIGALQSTSREAYVTVAGSLDLNSGAS 60

Query: 61  SELSNGRNKSFTGLTGATNKESLEAMVKSVGKESMQWGLAAGVYSGLTYGLKEARGAHDW 120
           SELSNGRNKSFTGLTGATNKESLEAMVK+VGKESMQWGLAAGVYSGLTYGLKEARGAHDW
Sbjct: 61  SELSNGRNKSFTGLTGATNKESLEAMVKNVGKESMQWGLAAGVYSGLTYGLKEARGAHDW 120

Query: 121 KNSAVAGAMTGVAVALTSDKPSHEHIVQYAITGAAVSTAANIFAGIF 168
           KNSAVAGAMTGVAVALTS+KPSHEHIVQYAITGAAVSTAANIFAGIF
Sbjct: 121 KNSAVAGAMTGVAVALTSEKPSHEHIVQYAITGAAVSTAANIFAGIF 167

BLAST of Carg05543 vs. NCBI nr
Match: XP_022959518.1 (outer envelope pore protein 16-2, chloroplastic-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 306.2 bits (783), Expect = 1.7e-79
Identity = 160/167 (95.81%), Postives = 163/167 (97.60%), Query Frame = 0

Query: 1   MGGKCFDNSGGGFLYDFGHPLVNRVADSFVKAAGIGALQSASREAYVTVAGSLDLNSGAS 60
           MGGKCF+NSGGGFLYDFGHPLVNRVADSFVKAAGIGALQS SREAYVTVAGSLDLNSGAS
Sbjct: 1   MGGKCFENSGGGFLYDFGHPLVNRVADSFVKAAGIGALQSTSREAYVTVAGSLDLNSGAS 60

Query: 61  SELSNGRNKSFTGLTGATNKESLEAMVKSVGKESMQWGLAAGVYSGLTYGLKEARGAHDW 120
           SELSNGRNKSF   TGATNKESLEAMVK+VGKESMQWGLAAGVYSGLTYGLKEARGAHDW
Sbjct: 61  SELSNGRNKSF---TGATNKESLEAMVKNVGKESMQWGLAAGVYSGLTYGLKEARGAHDW 120

Query: 121 KNSAVAGAMTGVAVALTSDKPSHEHIVQYAITGAAVSTAANIFAGIF 168
           KNSAVAGAMTGVAVALTS+KPSHEHIVQYAITGAAVSTAANIFAGIF
Sbjct: 121 KNSAVAGAMTGVAVALTSEKPSHEHIVQYAITGAAVSTAANIFAGIF 164

BLAST of Carg05543 vs. NCBI nr
Match: XP_023548561.1 (outer envelope pore protein 16-2, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 303.9 bits (777), Expect = 8.5e-79
Identity = 156/167 (93.41%), Postives = 160/167 (95.81%), Query Frame = 0

Query: 1   MGGKCFDNSGGGFLYDFGHPLVNRVADSFVKAAGIGALQSASREAYVTVAGSLDLNSGAS 60
           MGGKCF+NSGGGFLYDFGHPLVNRVADSFVKAAGIGALQSASREAY+TVAGSLD NSGA 
Sbjct: 1   MGGKCFENSGGGFLYDFGHPLVNRVADSFVKAAGIGALQSASREAYITVAGSLDSNSGAP 60

Query: 61  SELSNGRNKSFTGLTGATNKESLEAMVKSVGKESMQWGLAAGVYSGLTYGLKEARGAHDW 120
           SELS+ R KSF G TG TNKESLEAMVK+VGKESMQWGLAAGVYSGLTYGLKEARGAHDW
Sbjct: 61  SELSSSRKKSFPGFTGGTNKESLEAMVKNVGKESMQWGLAAGVYSGLTYGLKEARGAHDW 120

Query: 121 KNSAVAGAMTGVAVALTSDKPSHEHIVQYAITGAAVSTAANIFAGIF 168
           KNSAVAGAMTGVAVALTSDKPSHEHIVQYAITGAAVSTAANIFAGIF
Sbjct: 121 KNSAVAGAMTGVAVALTSDKPSHEHIVQYAITGAAVSTAANIFAGIF 167

BLAST of Carg05543 vs. NCBI nr
Match: XP_023006371.1 (outer envelope pore protein 16-2, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 299.3 bits (765), Expect = 2.1e-77
Identity = 153/167 (91.62%), Postives = 158/167 (94.61%), Query Frame = 0

Query: 1   MGGKCFDNSGGGFLYDFGHPLVNRVADSFVKAAGIGALQSASREAYVTVAGSLDLNSGAS 60
           MGGKC +N GGGFLYDFGHPL+NRVADSFVKAAGIGALQSASREAY+TVAGSLD NSGA 
Sbjct: 1   MGGKCLENCGGGFLYDFGHPLLNRVADSFVKAAGIGALQSASREAYITVAGSLDSNSGAP 60

Query: 61  SELSNGRNKSFTGLTGATNKESLEAMVKSVGKESMQWGLAAGVYSGLTYGLKEARGAHDW 120
           S LSNGRNK FTG TG TNKESLEAMVK+VGKESMQWGLAAGVYSGLTYGLKEARGAHDW
Sbjct: 61  SGLSNGRNKRFTGFTGGTNKESLEAMVKNVGKESMQWGLAAGVYSGLTYGLKEARGAHDW 120

Query: 121 KNSAVAGAMTGVAVALTSDKPSHEHIVQYAITGAAVSTAANIFAGIF 168
           KNSAVAGAMTGVAVALTSDKPSHEHIVQYAITGAAVSTA+NIFA IF
Sbjct: 121 KNSAVAGAMTGVAVALTSDKPSHEHIVQYAITGAAVSTASNIFASIF 167

BLAST of Carg05543 vs. ExPASy Swiss-Prot
Match: Q0WMZ5 (Outer envelope pore protein 16-2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OEP162 PE=2 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 1.4e-44
Identity = 96/159 (60.38%), Postives = 125/159 (78.62%), Query Frame = 0

Query: 14  LYDFGHPLVNRVADSFVKAAGIGALQSASREAYVTV---AGSLDLNSGASSELSNGRNKS 73
           L+D GHPL+NR+ADSFVKAAG+GALQ+ SREAY TV   AG    N G  SE++  +   
Sbjct: 21  LFDLGHPLLNRIADSFVKAAGVGALQAVSREAYFTVVDGAGFDSNNVGPPSEITGNKKHR 80

Query: 74  FTGLTGATNKESLEAMVKSVGKESMQWGLAAGVYSGLTYGLKEAR-GAHDWKNSAVAGAM 133
           F  L G ++K SL+A+VK+ GKES+QWGLAAG+YSG+TYG+ E R GAHDW+NSAVAGA+
Sbjct: 81  FPNLRGESSK-SLDALVKNTGKESLQWGLAAGLYSGITYGMTEVRGGAHDWRNSAVAGAL 140

Query: 134 TGVAVAL-TSDKPSHEHIVQYAITGAAVSTAANIFAGIF 168
           TG A+A+ TS++ SHE +VQ A+TGAA+STAAN+ + +F
Sbjct: 141 TGAAMAMTTSERTSHEQVVQSALTGAAISTAANLLSSVF 178

BLAST of Carg05543 vs. ExPASy Swiss-Prot
Match: Q9ZV24 (Outer envelope pore protein 16-1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OEP161 PE=1 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 3.4e-14
Identity = 48/145 (33.10%), Postives = 72/145 (49.66%), Query Frame = 0

Query: 16  DFGHPLVNRVADSFVKAAGIGALQSASREAYVTVAGSLDLNSGASSELSNGRNKSFTGLT 75
           D G+P +N   D+F+K   +G  +S + + Y  +                          
Sbjct: 20  DMGNPFLNLTVDAFLKIGAVGVTKSLAEDTYKAID------------------------K 79

Query: 76  GATNKESLEAMVKSVGKESMQWGLAAGVYSGLTYGLKEARGAHDWKNSAVAGAMTGVAVA 135
           G+ +K +LE  +K + KE + WG A GVY G  YG++  RG+ DWKN+ +AGA TG AV 
Sbjct: 80  GSLSKSTLEHALKKLCKEGVYWGAAGGVYIGTEYGIERIRGSRDWKNAMLAGAATG-AVL 139

Query: 136 LTSDKPSHEHIVQYAITGAAVSTAA 161
               K   + IV  AI G A++TA+
Sbjct: 140 SAVGKKGKDTIVIDAILGGALATAS 139

BLAST of Carg05543 vs. ExPASy Swiss-Prot
Match: Q41050 (Outer envelope pore protein 16, chloroplastic OS=Pisum sativum OX=3888 GN=OEP16 PE=1 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 2.2e-13
Identity = 45/145 (31.03%), Postives = 68/145 (46.90%), Query Frame = 0

Query: 16  DFGHPLVNRVADSFVKAAGIGALQSASREAYVTVAGSLDLNSGASSELSNGRNKSFTGLT 75
           D G+P +N   D F+K   + A +S + + +  +                          
Sbjct: 20  DMGNPFLNLTVDGFLKIGAVAATRSVAEDTFHIIR------------------------K 79

Query: 76  GATNKESLEAMVKSVGKESMQWGLAAGVYSGLTYGLKEARGAHDWKNSAVAGAMTGVAVA 135
           G+ +    E  +K + KE   WG  AGVY G+ YG++  RG  DWKN+   GA+TG  V+
Sbjct: 80  GSISSNDFEKSLKKMCKEGAYWGAIAGVYVGMEYGVERIRGTRDWKNAMFGGAVTGALVS 139

Query: 136 LTSDKPSHEHIVQYAITGAAVSTAA 161
             S+    + I   AITGAA++TAA
Sbjct: 140 AASNN-KKDKIAVDAITGAAIATAA 139

BLAST of Carg05543 vs. ExPASy Swiss-Prot
Match: Q9LZH8 (Outer envelope pore protein 16-4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OEP164 PE=2 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 1.4e-04
Identity = 23/63 (36.51%), Postives = 37/63 (58.73%), Query Frame = 0

Query: 76  GATNKESLEAMVKSVGKESMQWGLAAGVYSGLTYGLKEARGAHDWKNSAVAGAMTGVAVA 135
           G +       + KS+G+   Q GL +GV++    GL+  RG +DW N+ V GA+ G AVA
Sbjct: 42  GLSGVSQASFVAKSIGRFGFQCGLVSGVFTMTHCGLQRYRGKNDWVNALVGGAVAGAAVA 101

Query: 136 LTS 139
           +++
Sbjct: 102 IST 104

BLAST of Carg05543 vs. ExPASy TrEMBL
Match: A0A6J1H4R7 (outer envelope pore protein 16-2, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111460473 PE=4 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 3.6e-83
Identity = 163/167 (97.60%), Postives = 166/167 (99.40%), Query Frame = 0

Query: 1   MGGKCFDNSGGGFLYDFGHPLVNRVADSFVKAAGIGALQSASREAYVTVAGSLDLNSGAS 60
           MGGKCF+NSGGGFLYDFGHPLVNRVADSFVKAAGIGALQS SREAYVTVAGSLDLNSGAS
Sbjct: 1   MGGKCFENSGGGFLYDFGHPLVNRVADSFVKAAGIGALQSTSREAYVTVAGSLDLNSGAS 60

Query: 61  SELSNGRNKSFTGLTGATNKESLEAMVKSVGKESMQWGLAAGVYSGLTYGLKEARGAHDW 120
           SELSNGRNKSFTGLTGATNKESLEAMVK+VGKESMQWGLAAGVYSGLTYGLKEARGAHDW
Sbjct: 61  SELSNGRNKSFTGLTGATNKESLEAMVKNVGKESMQWGLAAGVYSGLTYGLKEARGAHDW 120

Query: 121 KNSAVAGAMTGVAVALTSDKPSHEHIVQYAITGAAVSTAANIFAGIF 168
           KNSAVAGAMTGVAVALTS+KPSHEHIVQYAITGAAVSTAANIFAGIF
Sbjct: 121 KNSAVAGAMTGVAVALTSEKPSHEHIVQYAITGAAVSTAANIFAGIF 167

BLAST of Carg05543 vs. ExPASy TrEMBL
Match: A0A6J1H663 (outer envelope pore protein 16-2, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111460473 PE=4 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 8.3e-80
Identity = 160/167 (95.81%), Postives = 163/167 (97.60%), Query Frame = 0

Query: 1   MGGKCFDNSGGGFLYDFGHPLVNRVADSFVKAAGIGALQSASREAYVTVAGSLDLNSGAS 60
           MGGKCF+NSGGGFLYDFGHPLVNRVADSFVKAAGIGALQS SREAYVTVAGSLDLNSGAS
Sbjct: 1   MGGKCFENSGGGFLYDFGHPLVNRVADSFVKAAGIGALQSTSREAYVTVAGSLDLNSGAS 60

Query: 61  SELSNGRNKSFTGLTGATNKESLEAMVKSVGKESMQWGLAAGVYSGLTYGLKEARGAHDW 120
           SELSNGRNKSF   TGATNKESLEAMVK+VGKESMQWGLAAGVYSGLTYGLKEARGAHDW
Sbjct: 61  SELSNGRNKSF---TGATNKESLEAMVKNVGKESMQWGLAAGVYSGLTYGLKEARGAHDW 120

Query: 121 KNSAVAGAMTGVAVALTSDKPSHEHIVQYAITGAAVSTAANIFAGIF 168
           KNSAVAGAMTGVAVALTS+KPSHEHIVQYAITGAAVSTAANIFAGIF
Sbjct: 121 KNSAVAGAMTGVAVALTSEKPSHEHIVQYAITGAAVSTAANIFAGIF 164

BLAST of Carg05543 vs. ExPASy TrEMBL
Match: A0A6J1KXL1 (outer envelope pore protein 16-2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111499121 PE=4 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 1.0e-77
Identity = 153/167 (91.62%), Postives = 158/167 (94.61%), Query Frame = 0

Query: 1   MGGKCFDNSGGGFLYDFGHPLVNRVADSFVKAAGIGALQSASREAYVTVAGSLDLNSGAS 60
           MGGKC +N GGGFLYDFGHPL+NRVADSFVKAAGIGALQSASREAY+TVAGSLD NSGA 
Sbjct: 1   MGGKCLENCGGGFLYDFGHPLLNRVADSFVKAAGIGALQSASREAYITVAGSLDSNSGAP 60

Query: 61  SELSNGRNKSFTGLTGATNKESLEAMVKSVGKESMQWGLAAGVYSGLTYGLKEARGAHDW 120
           S LSNGRNK FTG TG TNKESLEAMVK+VGKESMQWGLAAGVYSGLTYGLKEARGAHDW
Sbjct: 61  SGLSNGRNKRFTGFTGGTNKESLEAMVKNVGKESMQWGLAAGVYSGLTYGLKEARGAHDW 120

Query: 121 KNSAVAGAMTGVAVALTSDKPSHEHIVQYAITGAAVSTAANIFAGIF 168
           KNSAVAGAMTGVAVALTSDKPSHEHIVQYAITGAAVSTA+NIFA IF
Sbjct: 121 KNSAVAGAMTGVAVALTSDKPSHEHIVQYAITGAAVSTASNIFASIF 167

BLAST of Carg05543 vs. ExPASy TrEMBL
Match: A0A0A0KCC7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G307410 PE=4 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 8.3e-64
Identity = 136/177 (76.84%), Postives = 146/177 (82.49%), Query Frame = 0

Query: 1   MGGKCFDN----------SGGGFLYDFGHPLVNRVADSFVKAAGIGALQSASREAYVTVA 60
           MG +  DN           GG FLYD GHPL+NRVADSFVKAAGIGALQS SREAY TVA
Sbjct: 50  MGSRSIDNLSMVDEIRCSHGGTFLYDLGHPLLNRVADSFVKAAGIGALQSVSREAYFTVA 109

Query: 61  GSLDLNSGASSELSNGRNKSFTGLTGATNKESLEAMVKSVGKESMQWGLAAGVYSGLTYG 120
           GS+D N     ELS+ R + F GL G TNKESLEAMVKSVGKES+QWGLAAGVYSGLTYG
Sbjct: 110 GSVDSNIAPPPELSSMRKQRFPGLKGETNKESLEAMVKSVGKESIQWGLAAGVYSGLTYG 169

Query: 121 LKEARGAHDWKNSAVAGAMTGVAVALTSDKPSHEHIVQYAITGAAVSTAANIFAGIF 168
           LKEARGAHDWKNSA+AGA+TGVAVALT+DK SHEHIVQYAITGAA+STAANIFAGIF
Sbjct: 170 LKEARGAHDWKNSAIAGAVTGVAVALTADKSSHEHIVQYAITGAAMSTAANIFAGIF 226

BLAST of Carg05543 vs. ExPASy TrEMBL
Match: A0A1S3C7N2 (outer envelope pore protein 16-2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103497794 PE=4 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 3.2e-63
Identity = 136/177 (76.84%), Postives = 146/177 (82.49%), Query Frame = 0

Query: 1   MGGKCFDN----------SGGGFLYDFGHPLVNRVADSFVKAAGIGALQSASREAYVTVA 60
           MG +  DN            G FLYD GHPL+NRVADSFVKAAGIGALQS SREAY TVA
Sbjct: 1   MGSRSIDNLSLVDEIRCTHSGPFLYDLGHPLLNRVADSFVKAAGIGALQSVSREAYFTVA 60

Query: 61  GSLDLNSGASSELSNGRNKSFTGLTGATNKESLEAMVKSVGKESMQWGLAAGVYSGLTYG 120
           GS+D NS    ELS+ R + F GL G TNKESLEAMVKSVGKES+QWGLAAGVYSGLTYG
Sbjct: 61  GSVDSNSVPPPELSSIRKQRFPGLKGETNKESLEAMVKSVGKESIQWGLAAGVYSGLTYG 120

Query: 121 LKEARGAHDWKNSAVAGAMTGVAVALTSDKPSHEHIVQYAITGAAVSTAANIFAGIF 168
           LKEARGAHDWKNSA+AGA+TGVAVALTSD+ SHEHIVQYAITGAA+STAANIFAGIF
Sbjct: 121 LKEARGAHDWKNSAIAGAVTGVAVALTSDESSHEHIVQYAITGAAMSTAANIFAGIF 177

BLAST of Carg05543 vs. TAIR 10
Match: AT4G16160.1 (Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family protein )

HSP 1 Score: 183.3 bits (464), Expect = 1.6e-46
Identity = 95/157 (60.51%), Postives = 125/157 (79.62%), Query Frame = 0

Query: 14  LYDFGHPLVNRVADSFVKAAGIGALQSASREAYVTVAGSLDLNS-GASSELSNGRNKSFT 73
           L+D GHPL+NR+ADSFVKAAG+GALQ+ SREAY TV    D N+ G  SE++  +   F 
Sbjct: 21  LFDLGHPLLNRIADSFVKAAGVGALQAVSREAYFTVVDGFDSNNVGPPSEITGNKKHRFP 80

Query: 74  GLTGATNKESLEAMVKSVGKESMQWGLAAGVYSGLTYGLKEAR-GAHDWKNSAVAGAMTG 133
            L G ++K SL+A+VK+ GKES+QWGLAAG+YSG+TYG+ E R GAHDW+NSAVAGA+TG
Sbjct: 81  NLRGESSK-SLDALVKNTGKESLQWGLAAGLYSGITYGMTEVRGGAHDWRNSAVAGALTG 140

Query: 134 VAVAL-TSDKPSHEHIVQYAITGAAVSTAANIFAGIF 168
            A+A+ TS++ SHE +VQ A+TGAA+STAAN+ + +F
Sbjct: 141 AAMAMTTSERTSHEQVVQSALTGAAISTAANLLSSVF 176

BLAST of Carg05543 vs. TAIR 10
Match: AT4G16160.2 (Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family protein )

HSP 1 Score: 180.6 bits (457), Expect = 1.0e-45
Identity = 96/159 (60.38%), Postives = 125/159 (78.62%), Query Frame = 0

Query: 14  LYDFGHPLVNRVADSFVKAAGIGALQSASREAYVTV---AGSLDLNSGASSELSNGRNKS 73
           L+D GHPL+NR+ADSFVKAAG+GALQ+ SREAY TV   AG    N G  SE++  +   
Sbjct: 21  LFDLGHPLLNRIADSFVKAAGVGALQAVSREAYFTVVDGAGFDSNNVGPPSEITGNKKHR 80

Query: 74  FTGLTGATNKESLEAMVKSVGKESMQWGLAAGVYSGLTYGLKEAR-GAHDWKNSAVAGAM 133
           F  L G ++K SL+A+VK+ GKES+QWGLAAG+YSG+TYG+ E R GAHDW+NSAVAGA+
Sbjct: 81  FPNLRGESSK-SLDALVKNTGKESLQWGLAAGLYSGITYGMTEVRGGAHDWRNSAVAGAL 140

Query: 134 TGVAVAL-TSDKPSHEHIVQYAITGAAVSTAANIFAGIF 168
           TG A+A+ TS++ SHE +VQ A+TGAA+STAAN+ + +F
Sbjct: 141 TGAAMAMTTSERTSHEQVVQSALTGAAISTAANLLSSVF 178

BLAST of Carg05543 vs. TAIR 10
Match: AT2G28900.1 (outer plastid envelope protein 16-1 )

HSP 1 Score: 79.7 bits (195), Expect = 2.4e-15
Identity = 48/145 (33.10%), Postives = 72/145 (49.66%), Query Frame = 0

Query: 16  DFGHPLVNRVADSFVKAAGIGALQSASREAYVTVAGSLDLNSGASSELSNGRNKSFTGLT 75
           D G+P +N   D+F+K   +G  +S + + Y  +                          
Sbjct: 20  DMGNPFLNLTVDAFLKIGAVGVTKSLAEDTYKAID------------------------K 79

Query: 76  GATNKESLEAMVKSVGKESMQWGLAAGVYSGLTYGLKEARGAHDWKNSAVAGAMTGVAVA 135
           G+ +K +LE  +K + KE + WG A GVY G  YG++  RG+ DWKN+ +AGA TG AV 
Sbjct: 80  GSLSKSTLEHALKKLCKEGVYWGAAGGVYIGTEYGIERIRGSRDWKNAMLAGAATG-AVL 139

Query: 136 LTSDKPSHEHIVQYAITGAAVSTAA 161
               K   + IV  AI G A++TA+
Sbjct: 140 SAVGKKGKDTIVIDAILGGALATAS 139

BLAST of Carg05543 vs. TAIR 10
Match: AT3G62880.1 (Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family protein )

HSP 1 Score: 47.8 bits (112), Expect = 1.0e-05
Identity = 23/63 (36.51%), Postives = 37/63 (58.73%), Query Frame = 0

Query: 76  GATNKESLEAMVKSVGKESMQWGLAAGVYSGLTYGLKEARGAHDWKNSAVAGAMTGVAVA 135
           G +       + KS+G+   Q GL +GV++    GL+  RG +DW N+ V GA+ G AVA
Sbjct: 42  GLSGVSQASFVAKSIGRFGFQCGLVSGVFTMTHCGLQRYRGKNDWVNALVGGAVAGAAVA 101

Query: 136 LTS 139
           +++
Sbjct: 102 IST 104

BLAST of Carg05543 vs. TAIR 10
Match: AT3G62880.2 (Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family protein )

HSP 1 Score: 47.8 bits (112), Expect = 1.0e-05
Identity = 23/63 (36.51%), Postives = 37/63 (58.73%), Query Frame = 0

Query: 76  GATNKESLEAMVKSVGKESMQWGLAAGVYSGLTYGLKEARGAHDWKNSAVAGAMTGVAVA 135
           G +       + KS+G+   Q GL +GV++    GL+  RG +DW N+ V GA+ G AVA
Sbjct: 42  GLSGVSQASFVAKSIGRFGFQCGLVSGVFTMTHCGLQRYRGKNDWVNALVGGAVAGAAVA 101

Query: 136 LTS 139
           +++
Sbjct: 102 IST 104

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6575109.11.4e-84100.00Outer envelope pore protein 16-2, chloroplastic, partial [Cucurbita argyrosperma... [more]
XP_022959516.17.4e-8397.60outer envelope pore protein 16-2, chloroplastic-like isoform X1 [Cucurbita mosch... [more]
XP_022959518.11.7e-7995.81outer envelope pore protein 16-2, chloroplastic-like isoform X2 [Cucurbita mosch... [more]
XP_023548561.18.5e-7993.41outer envelope pore protein 16-2, chloroplastic-like [Cucurbita pepo subsp. pepo... [more]
XP_023006371.12.1e-7791.62outer envelope pore protein 16-2, chloroplastic-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q0WMZ51.4e-4460.38Outer envelope pore protein 16-2, chloroplastic OS=Arabidopsis thaliana OX=3702 ... [more]
Q9ZV243.4e-1433.10Outer envelope pore protein 16-1, chloroplastic OS=Arabidopsis thaliana OX=3702 ... [more]
Q410502.2e-1331.03Outer envelope pore protein 16, chloroplastic OS=Pisum sativum OX=3888 GN=OEP16 ... [more]
Q9LZH81.4e-0436.51Outer envelope pore protein 16-4, chloroplastic OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
A0A6J1H4R73.6e-8397.60outer envelope pore protein 16-2, chloroplastic-like isoform X1 OS=Cucurbita mos... [more]
A0A6J1H6638.3e-8095.81outer envelope pore protein 16-2, chloroplastic-like isoform X2 OS=Cucurbita mos... [more]
A0A6J1KXL11.0e-7791.62outer envelope pore protein 16-2, chloroplastic-like OS=Cucurbita maxima OX=3661... [more]
A0A0A0KCC78.3e-6476.84Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G307410 PE=4 SV=1[more]
A0A1S3C7N23.2e-6376.84outer envelope pore protein 16-2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC10... [more]
Match NameE-valueIdentityDescription
AT4G16160.11.6e-4660.51Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family... [more]
AT4G16160.21.0e-4560.38Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family... [more]
AT2G28900.12.4e-1533.10outer plastid envelope protein 16-1 [more]
AT3G62880.11.0e-0536.51Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family... [more]
AT3G62880.21.0e-0536.51Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family... [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF02466Tim17coord: 22..157
e-value: 3.9E-14
score: 53.0
NoneNo IPR availablePANTHERPTHR15371:SF1OUTER ENVELOPE PORE PROTEIN 16-2, CHLOROPLASTICcoord: 11..167
NoneNo IPR availablePANTHERPTHR15371TIM23coord: 11..167

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg05543-RACarg05543-RAmRNA