Carg05448 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg05448
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionbasic 7S globulin-like
LocationCarg_Chr17: 1481889 .. 1483139 (-)
RNA-Seq ExpressionCarg05448
SyntenyCarg05448
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGTTCCCGAATCTCGCCTTCTTCTTCTTCTTCTTCTTCTCTGTTTCTCAGCCCTCTTCCATGGCGGAGCTTTCTCTCTGGTCATTCCCGTCAACAAAGATTCCGTTACTAATCAGTATTTGGCCACGCTCTATCATGGCTCTCCGATTAAGCCTGTCCATCTCGCCGTTGATCTTGGCGGCCGGTTTCTCTGGATGGCTTGTGGTGGCTCTTCCTCCAGCCGGCCGATTCTTAGTCGGTCTATTCAGTGCATAGCGGCGGTTGGTGGGGGTAGATCTGGGTCTTCTGGCAGCGCGTGTGATGTAATTGCTGGGAATCCGTTTGGGGATTTGGAAGGGAAGGCGGTGCTGGTTGAGGATACGGTCGCCGTGAGGTCTTTGGACCGGTCAACGGCGGCGGTGATTGTTGCGCTTCATTCTTGTGCTCCGAGGTTTCTTTTACAAGGTTTGGCGAAGTCGGCTAAAGGAGTTTTGGGGCTTGGCCGGAATCAAATCTCGCTTCCGGCTCAGATCGCGACTGAGCTCGGTTTTCACCGGCGATTTTCCGTTTGTCTTTCGTCGACGAACGGCGTTGTTTTTCCGGATAGTGGAAACCTAGACTCTGTTTATGGATCTGAGATTTCGAGTTCTTTGACTTACACGCCGATTTTGACGAAGAAAATCGACGCCTCGCAGTCGCCGGAGTATTTCATCAACGTTAAAGCCATCAAAGTCGACGGTAACCGCCTCGATCTGAACAAGTCGCTTCTCGATTTGGATGGCAGAAGCGGCGGAGGAGGCGGCGGAGGGACGAGATTAAGCACGGTTGTTCCATACACCACACTAGAGAGTTCCATCTTCAACTCTCTGACGGCGGCTTTCAGAGCAGCAGCGGCGGCGATGAACATGAAAGAAGTGGCGCCGGTGGCGCCATTTGAGGCGTGTTTTGAATCGGAAAACATGGAGATGACAGCGACAGGGCCGAAAGTGCCGGAGATTGAACTGATTTTGCAGAGTGAAATGGTGGGATGGAAAATTAATGGAAGAAATTCAATGGTGAAGGTGAACGATGAAACTCTGTGTTTAGGGTTCGTCGACGGTGGATTGAAGCCAAGAAACGCCGTCGTTTTGGGAGGGTATCAAATGGAAGATATCGTCCTGGATTTCGACATGGGGACTTCCATGCTCGGATTTAGCTCCTCTTTGTTGCAGAGGAAGAAATCTTGCTCGGAATTTTCGCCGGAAAATTTACCGATCAGAGCCGGATGA

mRNA sequence

ATGGCCGTTCCCGAATCTCGCCTTCTTCTTCTTCTTCTTCTTCTCTGTTTCTCAGCCCTCTTCCATGGCGGAGCTTTCTCTCTGGTCATTCCCGTCAACAAAGATTCCGTTACTAATCAGTATTTGGCCACGCTCTATCATGGCTCTCCGATTAAGCCTGTCCATCTCGCCGTTGATCTTGGCGGCCGGTTTCTCTGGATGGCTTGTGGTGGCTCTTCCTCCAGCCGGCCGATTCTTAGTCGGTCTATTCAGTGCATAGCGGCGGTTGGTGGGGGTAGATCTGGGTCTTCTGGCAGCGCGTGTGATGTAATTGCTGGGAATCCGTTTGGGGATTTGGAAGGGAAGGCGGTGCTGGTTGAGGATACGGTCGCCGTGAGGTCTTTGGACCGGTCAACGGCGGCGGTGATTGTTGCGCTTCATTCTTGTGCTCCGAGGTTTCTTTTACAAGGTTTGGCGAAGTCGGCTAAAGGAGTTTTGGGGCTTGGCCGGAATCAAATCTCGCTTCCGGCTCAGATCGCGACTGAGCTCGGTTTTCACCGGCGATTTTCCGTTTGTCTTTCGTCGACGAACGGCGTTGTTTTTCCGGATAGTGGAAACCTAGACTCTGTTTATGGATCTGAGATTTCGAGTTCTTTGACTTACACGCCGATTTTGACGAAGAAAATCGACGCCTCGCAGTCGCCGGAGTATTTCATCAACGTTAAAGCCATCAAAGTCGACGGTAACCGCCTCGATCTGAACAAGTCGCTTCTCGATTTGGATGGCAGAAGCGGCGGAGGAGGCGGCGGAGGGACGAGATTAAGCACGGTTGTTCCATACACCACACTAGAGAGTTCCATCTTCAACTCTCTGACGGCGGCTTTCAGAGCAGCAGCGGCGGCGATGAACATGAAAGAAGTGGCGCCGGTGGCGCCATTTGAGGCGTGTTTTGAATCGGAAAACATGGAGATGACAGCGACAGGGCCGAAAGTGCCGGAGATTGAACTGATTTTGCAGAGTGAAATGGTGGGATGGAAAATTAATGGAAGAAATTCAATGGTGAAGGTGAACGATGAAACTCTGTGTTTAGGGTTCGTCGACGGTGGATTGAAGCCAAGAAACGCCGTCGTTTTGGGAGGGTATCAAATGGAAGATATCGTCCTGGATTTCGACATGGGGACTTCCATGCTCGGATTTAGCTCCTCTTTGTTGCAGAGGAAGAAATCTTGCTCGGAATTTTCGCCGGAAAATTTACCGATCAGAGCCGGATGA

Coding sequence (CDS)

ATGGCCGTTCCCGAATCTCGCCTTCTTCTTCTTCTTCTTCTTCTCTGTTTCTCAGCCCTCTTCCATGGCGGAGCTTTCTCTCTGGTCATTCCCGTCAACAAAGATTCCGTTACTAATCAGTATTTGGCCACGCTCTATCATGGCTCTCCGATTAAGCCTGTCCATCTCGCCGTTGATCTTGGCGGCCGGTTTCTCTGGATGGCTTGTGGTGGCTCTTCCTCCAGCCGGCCGATTCTTAGTCGGTCTATTCAGTGCATAGCGGCGGTTGGTGGGGGTAGATCTGGGTCTTCTGGCAGCGCGTGTGATGTAATTGCTGGGAATCCGTTTGGGGATTTGGAAGGGAAGGCGGTGCTGGTTGAGGATACGGTCGCCGTGAGGTCTTTGGACCGGTCAACGGCGGCGGTGATTGTTGCGCTTCATTCTTGTGCTCCGAGGTTTCTTTTACAAGGTTTGGCGAAGTCGGCTAAAGGAGTTTTGGGGCTTGGCCGGAATCAAATCTCGCTTCCGGCTCAGATCGCGACTGAGCTCGGTTTTCACCGGCGATTTTCCGTTTGTCTTTCGTCGACGAACGGCGTTGTTTTTCCGGATAGTGGAAACCTAGACTCTGTTTATGGATCTGAGATTTCGAGTTCTTTGACTTACACGCCGATTTTGACGAAGAAAATCGACGCCTCGCAGTCGCCGGAGTATTTCATCAACGTTAAAGCCATCAAAGTCGACGGTAACCGCCTCGATCTGAACAAGTCGCTTCTCGATTTGGATGGCAGAAGCGGCGGAGGAGGCGGCGGAGGGACGAGATTAAGCACGGTTGTTCCATACACCACACTAGAGAGTTCCATCTTCAACTCTCTGACGGCGGCTTTCAGAGCAGCAGCGGCGGCGATGAACATGAAAGAAGTGGCGCCGGTGGCGCCATTTGAGGCGTGTTTTGAATCGGAAAACATGGAGATGACAGCGACAGGGCCGAAAGTGCCGGAGATTGAACTGATTTTGCAGAGTGAAATGGTGGGATGGAAAATTAATGGAAGAAATTCAATGGTGAAGGTGAACGATGAAACTCTGTGTTTAGGGTTCGTCGACGGTGGATTGAAGCCAAGAAACGCCGTCGTTTTGGGAGGGTATCAAATGGAAGATATCGTCCTGGATTTCGACATGGGGACTTCCATGCTCGGATTTAGCTCCTCTTTGTTGCAGAGGAAGAAATCTTGCTCGGAATTTTCGCCGGAAAATTTACCGATCAGAGCCGGATGA

Protein sequence

MAVPESRLLLLLLLLCFSALFHGGAFSLVIPVNKDSVTNQYLATLYHGSPIKPVHLAVDLGGRFLWMACGGSSSSRPILSRSIQCIAAVGGGRSGSSGSACDVIAGNPFGDLEGKAVLVEDTVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSAKGVLGLGRNQISLPAQIATELGFHRRFSVCLSSTNGVVFPDSGNLDSVYGSEISSSLTYTPILTKKIDASQSPEYFINVKAIKVDGNRLDLNKSLLDLDGRSGGGGGGGTRLSTVVPYTTLESSIFNSLTAAFRAAAAAMNMKEVAPVAPFEACFESENMEMTATGPKVPEIELILQSEMVGWKINGRNSMVKVNDETLCLGFVDGGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKKSCSEFSPENLPIRAG
Homology
BLAST of Carg05448 vs. NCBI nr
Match: KAG7013583.1 (Basic 7S globulin, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 806.6 bits (2082), Expect = 1.0e-229
Identity = 416/416 (100.00%), Postives = 416/416 (100.00%), Query Frame = 0

Query: 1   MAVPESRLLLLLLLLCFSALFHGGAFSLVIPVNKDSVTNQYLATLYHGSPIKPVHLAVDL 60
           MAVPESRLLLLLLLLCFSALFHGGAFSLVIPVNKDSVTNQYLATLYHGSPIKPVHLAVDL
Sbjct: 1   MAVPESRLLLLLLLLCFSALFHGGAFSLVIPVNKDSVTNQYLATLYHGSPIKPVHLAVDL 60

Query: 61  GGRFLWMACGGSSSSRPILSRSIQCIAAVGGGRSGSSGSACDVIAGNPFGDLEGKAVLVE 120
           GGRFLWMACGGSSSSRPILSRSIQCIAAVGGGRSGSSGSACDVIAGNPFGDLEGKAVLVE
Sbjct: 61  GGRFLWMACGGSSSSRPILSRSIQCIAAVGGGRSGSSGSACDVIAGNPFGDLEGKAVLVE 120

Query: 121 DTVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSAKGVLGLGRNQISLPAQIATELGFHR 180
           DTVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSAKGVLGLGRNQISLPAQIATELGFHR
Sbjct: 121 DTVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSAKGVLGLGRNQISLPAQIATELGFHR 180

Query: 181 RFSVCLSSTNGVVFPDSGNLDSVYGSEISSSLTYTPILTKKIDASQSPEYFINVKAIKVD 240
           RFSVCLSSTNGVVFPDSGNLDSVYGSEISSSLTYTPILTKKIDASQSPEYFINVKAIKVD
Sbjct: 181 RFSVCLSSTNGVVFPDSGNLDSVYGSEISSSLTYTPILTKKIDASQSPEYFINVKAIKVD 240

Query: 241 GNRLDLNKSLLDLDGRSGGGGGGGTRLSTVVPYTTLESSIFNSLTAAFRAAAAAMNMKEV 300
           GNRLDLNKSLLDLDGRSGGGGGGGTRLSTVVPYTTLESSIFNSLTAAFRAAAAAMNMKEV
Sbjct: 241 GNRLDLNKSLLDLDGRSGGGGGGGTRLSTVVPYTTLESSIFNSLTAAFRAAAAAMNMKEV 300

Query: 301 APVAPFEACFESENMEMTATGPKVPEIELILQSEMVGWKINGRNSMVKVNDETLCLGFVD 360
           APVAPFEACFESENMEMTATGPKVPEIELILQSEMVGWKINGRNSMVKVNDETLCLGFVD
Sbjct: 301 APVAPFEACFESENMEMTATGPKVPEIELILQSEMVGWKINGRNSMVKVNDETLCLGFVD 360

Query: 361 GGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKKSCSEFSPENLPIRAG 417
           GGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKKSCSEFSPENLPIRAG
Sbjct: 361 GGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKKSCSEFSPENLPIRAG 416

BLAST of Carg05448 vs. NCBI nr
Match: KAG6575012.1 (putative aspartic proteinase GIP2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 786.2 bits (2029), Expect = 1.4e-223
Identity = 408/416 (98.08%), Postives = 410/416 (98.56%), Query Frame = 0

Query: 1   MAVPESRLLLLLLLLCFSALFHGGAFSLVIPVNKDSVTNQYLATLYHGSPIKPVHLAVDL 60
           MAVPESRLLLLLLLLCFSALFHGGAFSLVIPVNKDSVTNQYLATLYHGSPIKPVHLAVDL
Sbjct: 1   MAVPESRLLLLLLLLCFSALFHGGAFSLVIPVNKDSVTNQYLATLYHGSPIKPVHLAVDL 60

Query: 61  GGRFLWMACGGSSSSRPILSRSIQCIAAVGGGRSGSSGSACDVIAGNPFGDLEGKAVLVE 120
           GGRFLWMACGGSSSSRPILSRSIQCIAAVGGGRSGSSGSACDVIAGNPFGDLEGKAVLVE
Sbjct: 61  GGRFLWMACGGSSSSRPILSRSIQCIAAVGGGRSGSSGSACDVIAGNPFGDLEGKAVLVE 120

Query: 121 DTVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSAKGVLGLGRNQISLPAQIATELGFHR 180
           DTVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSAKGVLGLGRNQISLPAQIATELGFHR
Sbjct: 121 DTVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSAKGVLGLGRNQISLPAQIATELGFHR 180

Query: 181 RFSVCLSSTNGVVFPDSGNLDSVYGSEISSSLTYTPILTKKIDASQSPEYFINVKAIKVD 240
           RFSVCLSSTNGVVFPDSGNLDSVYGSEISSSLTYTPILTKKIDASQSP+YFINVKAIKVD
Sbjct: 181 RFSVCLSSTNGVVFPDSGNLDSVYGSEISSSLTYTPILTKKIDASQSPKYFINVKAIKVD 240

Query: 241 GNRLDLNKSLLDLDGRSGGGGGGGTRLSTVVPYTTLESSIFNSLTAAFRAAAAAMNMKEV 300
           GNRLDLNKSLLDLD    GG GGGTRLSTVVPYTTLESSIFNSLT AFRAAAAA+NMKEV
Sbjct: 241 GNRLDLNKSLLDLD----GGSGGGTRLSTVVPYTTLESSIFNSLTVAFRAAAAAINMKEV 300

Query: 301 APVAPFEACFESENMEMTATGPKVPEIELILQSEMVGWKINGRNSMVKVNDETLCLGFVD 360
           APVAPFEACFESENMEMTATGPKVPEIELILQSEMVGWKINGRNSMVKVNDETLCLGFVD
Sbjct: 301 APVAPFEACFESENMEMTATGPKVPEIELILQSEMVGWKINGRNSMVKVNDETLCLGFVD 360

Query: 361 GGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKKSCSEFSPENLPIRAG 417
           GGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKKSCSEFSPENLPIRAG
Sbjct: 361 GGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKKSCSEFSPENLPIRAG 412

BLAST of Carg05448 vs. NCBI nr
Match: XP_022959401.1 (basic 7S globulin-like [Cucurbita moschata])

HSP 1 Score: 783.1 bits (2021), Expect = 1.2e-222
Identity = 409/417 (98.08%), Postives = 411/417 (98.56%), Query Frame = 0

Query: 1   MAVPESRLLLLL-LLLCFSALFHGGAFSLVIPVNKDSVTNQYLATLYHGSPIKPVHLAVD 60
           MAVPESRLLLLL LLLCFSALFHGGAFSLVIPVNKDSVTNQYLATLYHGSPIKPVHLAVD
Sbjct: 1   MAVPESRLLLLLRLLLCFSALFHGGAFSLVIPVNKDSVTNQYLATLYHGSPIKPVHLAVD 60

Query: 61  LGGRFLWMACGGSSSSRPILSRSIQCIAAVGGGRSGSSGSACDVIAGNPFGDLEGKAVLV 120
           LGG+FLWMACGGSSSSRPILSRSIQCIAAVGGGRSGSSGSACDVIAGNPFGDLEGKAVLV
Sbjct: 61  LGGQFLWMACGGSSSSRPILSRSIQCIAAVGGGRSGSSGSACDVIAGNPFGDLEGKAVLV 120

Query: 121 EDTVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSAKGVLGLGRNQISLPAQIATELGFH 180
           EDTVAVRSLDRSTAAVIVALHSCAPRFLLQGLA+SAKGVLGLGRNQISLPAQIATELGFH
Sbjct: 121 EDTVAVRSLDRSTAAVIVALHSCAPRFLLQGLAESAKGVLGLGRNQISLPAQIATELGFH 180

Query: 181 RRFSVCLSSTNGVVFPDSGNLDSVYGSEISSSLTYTPILTKKIDASQSPEYFINVKAIKV 240
           RRFSVCLSSTNGVVFPDSGNLDSVYGSEISSSLTYTPILTKKIDASQSPEYFINVKAIKV
Sbjct: 181 RRFSVCLSSTNGVVFPDSGNLDSVYGSEISSSLTYTPILTKKIDASQSPEYFINVKAIKV 240

Query: 241 DGNRLDLNKSLLDLDGRSGGGGGGGTRLSTVVPYTTLESSIFNSLTAAFRAAAAAMNMKE 300
           DGNRLDLNKSLLDLD    GG GGGTRLSTVVPYTTLESSIFNSLTAAFRAAAAAMNMKE
Sbjct: 241 DGNRLDLNKSLLDLD----GGSGGGTRLSTVVPYTTLESSIFNSLTAAFRAAAAAMNMKE 300

Query: 301 VAPVAPFEACFESENMEMTATGPKVPEIELILQSEMVGWKINGRNSMVKVNDETLCLGFV 360
           VAPVAPFEACFESENMEMTATGPKVPEIELILQSEMVGWKINGRNSMVKVNDETLCLGFV
Sbjct: 301 VAPVAPFEACFESENMEMTATGPKVPEIELILQSEMVGWKINGRNSMVKVNDETLCLGFV 360

Query: 361 DGGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKKSCSEFSPENLPIRAG 417
           DGGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKKSCSEFSPENLPIRAG
Sbjct: 361 DGGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKKSCSEFSPENLPIRAG 413

BLAST of Carg05448 vs. NCBI nr
Match: XP_023006289.1 (basic 7S globulin-like [Cucurbita maxima])

HSP 1 Score: 775.8 bits (2002), Expect = 1.9e-220
Identity = 405/416 (97.36%), Postives = 407/416 (97.84%), Query Frame = 0

Query: 1   MAVPESRLLLLLLLLCFSALFHGGAFSLVIPVNKDSVTNQYLATLYHGSPIKPVHLAVDL 60
           MAVP+SR  LLLLLLCFSALFHGGAFSLVIPVNKDSVTNQYLATLYHGS IKPVHLAVDL
Sbjct: 1   MAVPKSR-FLLLLLLCFSALFHGGAFSLVIPVNKDSVTNQYLATLYHGSQIKPVHLAVDL 60

Query: 61  GGRFLWMACGGSSSSRPILSRSIQCIAAVGGGRSGSSGSACDVIAGNPFGDLEGKAVLVE 120
           GGRFLWMACGGSSSSRPILSRSIQCIAAVGGGRSGSSGSACDVIAGNPFGDLEGKAVLVE
Sbjct: 61  GGRFLWMACGGSSSSRPILSRSIQCIAAVGGGRSGSSGSACDVIAGNPFGDLEGKAVLVE 120

Query: 121 DTVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSAKGVLGLGRNQISLPAQIATELGFHR 180
           DTVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSAKGVLGLGRNQISLPAQIATELGFHR
Sbjct: 121 DTVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSAKGVLGLGRNQISLPAQIATELGFHR 180

Query: 181 RFSVCLSSTNGVVFPDSGNLDSVYGSEISSSLTYTPILTKKIDASQSPEYFINVKAIKVD 240
           RFSVCLSSTNGVVFPD+GNLDSVYGSEISSSLTYTPILTKK DASQSPEYFINVKAIKVD
Sbjct: 181 RFSVCLSSTNGVVFPDTGNLDSVYGSEISSSLTYTPILTKKTDASQSPEYFINVKAIKVD 240

Query: 241 GNRLDLNKSLLDLDGRSGGGGGGGTRLSTVVPYTTLESSIFNSLTAAFRAAAAAMNMKEV 300
           GNRLDLNKSLLDLD    GGGGGGTRLSTVVPYTTL SSIFNSLTAAFRAAAAAMNMKEV
Sbjct: 241 GNRLDLNKSLLDLD----GGGGGGTRLSTVVPYTTLGSSIFNSLTAAFRAAAAAMNMKEV 300

Query: 301 APVAPFEACFESENMEMTATGPKVPEIELILQSEMVGWKINGRNSMVKVNDETLCLGFVD 360
           APVAPFEACFESENMEMTATGPKVPEIELILQSEMVGWKINGRNSMVKVNDETLCLGFVD
Sbjct: 301 APVAPFEACFESENMEMTATGPKVPEIELILQSEMVGWKINGRNSMVKVNDETLCLGFVD 360

Query: 361 GGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKKSCSEFSPENLPIRAG 417
           GGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKKSCSEFSPENLPIRAG
Sbjct: 361 GGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKKSCSEFSPENLPIRAG 411

BLAST of Carg05448 vs. NCBI nr
Match: XP_023549177.1 (basic 7S globulin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 774.6 bits (1999), Expect = 4.2e-220
Identity = 404/416 (97.12%), Postives = 405/416 (97.36%), Query Frame = 0

Query: 1   MAVPESRLLLLLLLLCFSALFHGGAFSLVIPVNKDSVTNQYLATLYHGSPIKPVHLAVDL 60
           MAVP+SR   LLLLLCFSALFHGGAFS VIPVNKDSVTNQYLATLYHGSPIKPVHLAVDL
Sbjct: 1   MAVPKSR--FLLLLLCFSALFHGGAFSPVIPVNKDSVTNQYLATLYHGSPIKPVHLAVDL 60

Query: 61  GGRFLWMACGGSSSSRPILSRSIQCIAAVGGGRSGSSGSACDVIAGNPFGDLEGKAVLVE 120
           GGRFLWMACGGSSS RPILSRSIQCIAAVGGGRSGSSGSACDVIAGNPFGDLEGKAVLVE
Sbjct: 61  GGRFLWMACGGSSSRRPILSRSIQCIAAVGGGRSGSSGSACDVIAGNPFGDLEGKAVLVE 120

Query: 121 DTVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSAKGVLGLGRNQISLPAQIATELGFHR 180
           DTVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSAKGVLGLGRNQISLPAQIATELGFHR
Sbjct: 121 DTVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSAKGVLGLGRNQISLPAQIATELGFHR 180

Query: 181 RFSVCLSSTNGVVFPDSGNLDSVYGSEISSSLTYTPILTKKIDASQSPEYFINVKAIKVD 240
           RFSVCLSSTNGVVFPDSGNLDSVYGSEISSSLTYTPILTKKIDASQSPEYFINVKAIKVD
Sbjct: 181 RFSVCLSSTNGVVFPDSGNLDSVYGSEISSSLTYTPILTKKIDASQSPEYFINVKAIKVD 240

Query: 241 GNRLDLNKSLLDLDGRSGGGGGGGTRLSTVVPYTTLESSIFNSLTAAFRAAAAAMNMKEV 300
           GNRLDLNKSLLDLD    GG GGGTRLSTVVPYTTLESSIFNSLTAAFR AAAAMNMKEV
Sbjct: 241 GNRLDLNKSLLDLD----GGSGGGTRLSTVVPYTTLESSIFNSLTAAFRVAAAAMNMKEV 300

Query: 301 APVAPFEACFESENMEMTATGPKVPEIELILQSEMVGWKINGRNSMVKVNDETLCLGFVD 360
           APVAPFEACFESENMEMTATGPKVPEIELILQSEMVGWKINGRNSMVKVNDETLCLGFVD
Sbjct: 301 APVAPFEACFESENMEMTATGPKVPEIELILQSEMVGWKINGRNSMVKVNDETLCLGFVD 360

Query: 361 GGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKKSCSEFSPENLPIRAG 417
           GGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKKSCSEFSPENLPIRAG
Sbjct: 361 GGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKKSCSEFSPENLPIRAG 410

BLAST of Carg05448 vs. ExPASy Swiss-Prot
Match: P0DO21 (Probable aspartic proteinase GIP2 OS=Nicotiana benthamiana OX=4100 GN=GIP2 PE=1 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 1.6e-76
Identity = 168/407 (41.28%), Postives = 243/407 (59.71%), Query Frame = 0

Query: 28  LVIPVNKDSVTNQYLATLYHGSPIKPVHLAVDLGGRFLWMACGG---SSSSRPILSRSIQ 87
           L++P+ KD++T QYL  +   +P+ PV L +DLGG+FLW+ C     SS+ RP   RS Q
Sbjct: 34  LILPITKDALTLQYLTQIQQRTPLVPVSLTLDLGGQFLWVDCDQGYVSSTYRPARCRSAQ 93

Query: 88  C-IAAVGGG--------RSGSSGSACDVIAGNPFGDLEGKAVLVEDTVAVRSLDRSTAAV 147
           C +A  G G        + G + + C ++  N          L  DTV V+S +      
Sbjct: 94  CSLAGAGSGCGQCFSPPKPGCNNNTCGLLPDNTITRTATSGELASDTVQVQSSNGKNPGR 153

Query: 148 IVA----LHSCAPRFLLQGLAKSAKGVLGLGRNQISLPAQIATELGFHRRFSVCLSS--- 207
            V+    L  C   FLL+GLA   KG+ GLGR +ISLP+Q + E  F R+F+VCLSS   
Sbjct: 154 HVSDKDFLFVCGSTFLLEGLASGVKGMAGLGRTRISLPSQFSAEFSFPRKFAVCLSSSTN 213

Query: 208 TNGVVFPDSGNLDSVYGSEISSS-LTYTPILTKKIDASQ-------SPEYFINVKAIKVD 267
           + GVV    G    +   E +++  +YTP+    +  +        S EYFI VK+IK++
Sbjct: 214 SKGVVLFGDGPYTFLPNREFANNDFSYTPLFINPVSTASAFSSREPSSEYFIGVKSIKIN 273

Query: 268 GNRLDLNKSLLDLDGRSGGGGGGGTRLSTVVPYTTLESSIFNSLTAAFRAAAAAMNMKEV 327
              + +N +LL +D +    G GGT++STV PYT LE+SI+N++T  F      +N+  V
Sbjct: 274 EKVVPINTTLLSIDNQ----GVGGTKISTVNPYTILETSIYNAVTNFF--VKELVNITRV 333

Query: 328 APVAPFEACFESENMEMTATGPKVPEIELILQSEMVGWKINGRNSMVKVNDETLCLGFVD 387
           A VAPF ACF+S N+  T  GP VP I+L+LQ+E V W+I G NSMV+V++  LCLGFVD
Sbjct: 334 ASVAPFRACFDSRNIASTRVGPAVPSIDLVLQNENVFWRIFGANSMVQVSENVLCLGFVD 393

Query: 388 GGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKKSCSEFS 408
           GG+ PR ++V+GGY +ED +L FD+  S LGF+SS+L R+ +C+ F+
Sbjct: 394 GGVSPRTSIVVGGYTIEDNLLQFDLARSRLGFTSSILFRQTTCANFN 434

BLAST of Carg05448 vs. ExPASy Swiss-Prot
Match: I1JNS6 (Probable aspartic proteinase GIP1 OS=Glycine max OX=3847 GN=GIP1 PE=1 SV=2)

HSP 1 Score: 221.5 bits (563), Expect = 1.8e-56
Identity = 144/420 (34.29%), Postives = 228/420 (54.29%), Query Frame = 0

Query: 10  LLLLLLCFSALFHGGAFSLVIPVNKDSVTNQYLATLYHGSPIKPVHLAVDLGGRFLWMAC 69
           L +L L  +  F      L+ P++KD  T  Y  +++  +P++P  L + LG    W+ C
Sbjct: 13  LAILFLFLTPTFQ---IPLIAPISKDDTTQLYTLSVFLKTPLQPTKLHLHLGSSLSWVLC 72

Query: 70  GG---SSSSRPILSRSIQCIAAVGGGRSGSSGSACDVIAGNPFGDLEGKAVLVEDTVAVR 129
                SSSS  I   +  C +      S +S S C +   NP          + D++A+ 
Sbjct: 73  DSTYTSSSSHHIPCNTPLCNSFPSNACSNNS-SLCALFPENPVTRNTLLDTALIDSLALP 132

Query: 130 SLDRSTAAVIVA--LHSCAPRFLLQGLAKSAKGVLGLGRNQISLPAQIATELGFHRRFSV 189
           + D S++ V+++  + SCA   LLQGLA +A G+  LGR+  SLPAQI+T L   R F++
Sbjct: 133 TYDASSSLVLISDFIFSCATAHLLQGLAANALGLASLGRSNYSLPAQISTSLTSPRSFTL 192

Query: 190 CL----SSTNGVVFPDSGNLDSVYGSEISSSLTYTPILTKKI-------DASQSPEYFIN 249
           CL    ++T   +F  + +   ++ S+I   LTYT ++   +       +   S EYFIN
Sbjct: 193 CLPASSANTGAAIFASTAS-SFLFSSKI--DLTYTQLIVNPVADTVVTDNPQPSDEYFIN 252

Query: 250 VKAIKVDGNRLDLNKSLLDLDGRSGGGGGGGTRLSTVVPYTTLESSIFNSLTAAFRAAAA 309
           + +IK++G  L +N S+L +D      G GGT++ST  PYT LE+SI+      F   ++
Sbjct: 253 LTSIKINGKPLYINSSILTVDQT----GFGGTKISTAEPYTVLETSIYRLFVQRFVNESS 312

Query: 310 AMNMKEVAPVAPFEACFESENMEMTATGPKVPEIELILQSEMVGWKINGRNSMVKV---N 369
           A N+     V PF  C+ + ++  T  GP VP ++L++ SE V W+I G NSMV+V    
Sbjct: 313 AFNLTVTEAVEPFGVCYPAGDLTETRVGPAVPTVDLVMHSEDVFWRIFGGNSMVRVAKGG 372

Query: 370 DETLCLGFVDGGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKKSCSEFSPEN 411
            +  CLGFVDGG + R  +V+GG+Q+ED ++ FD+ ++  GF+S+LL +   CS     N
Sbjct: 373 VDVWCLGFVDGGTRGRTPIVIGGHQLEDNLMQFDLDSNRFGFTSTLLLQDAKCSNLKVNN 421

BLAST of Carg05448 vs. ExPASy Swiss-Prot
Match: P82952 (Gamma conglutin 1 OS=Prunus dulcis OX=3755 GN=Cgamma1 PE=1 SV=2)

HSP 1 Score: 221.1 bits (562), Expect = 2.4e-56
Identity = 144/401 (35.91%), Postives = 215/401 (53.62%), Query Frame = 0

Query: 28  LVIPVNKDSVTNQYLATLYHGSPIKPVHLAVDLGGRFLWMACGG---SSSSRPILSRSIQ 87
           LV+ V KD  TN ++  ++  +P+      +DL GRFL + C     SS+ +  +  S Q
Sbjct: 38  LVLKVQKDRATNLHVVQIHKRTPLVQFPFVIDLTGRFLSVNCENQYTSSTYKAPVCHSSQ 97

Query: 88  CIAA---------VGGGRSGSSGSACDVIAGNPFGDLEGKAVLVEDTVAVRSLDRSTAAV 147
           C  A             R G   +AC ++  NP      +  L ED + + S   S+   
Sbjct: 98  CARANSHTCRTCSSSKTRPGCHTNACGLLTTNPVTQQSAQGELAEDVLKIPSTQGSSPGP 157

Query: 148 IVA----LHSCAPRFLLQ-GLAKSAKGVLGLGRNQISLPAQIATELGFHRRFSVCLSST- 207
           +V     L +CAP  +LQ GL K+ +GV GLG + ISLP Q+A+  GF  +F+VCL+S+ 
Sbjct: 158 MVTYPHFLFACAPSNILQKGLPKNVQGVAGLGHSPISLPYQLASHFGFPPKFAVCLTSSP 217

Query: 208 --NGVVFPDSGNLDSVYGSEISSSLTYTPILTKKIDASQSPEYFINVKAIKVDGNRLDLN 267
             NG VF   G      G ++S  LTY P         Q  EY+INV++ K+       N
Sbjct: 218 GKNGAVFFGEGPYFMKPGIDVSRQLTYAP-----FTIGQQGEYYINVQSFKI-------N 277

Query: 268 KSLLDLDGRSGGGGGGGTRLSTVVPYTTLESSIFNSLTAAFRAAAAAMNMKEVAPVAPFE 327
            ++L    +   GG GG  +ST  PYTTL++ IF +L   F        +  V PVAPF 
Sbjct: 278 NAMLPSIPK---GGFGGAMISTTTPYTTLQTPIFRALNQLF--MNQLRGVPHVKPVAPFG 337

Query: 328 ACFESENMEMTATGPKVPEIELILQSEM-VGWKINGRNSMVKVNDETLCLGFVDGGLKPR 387
           ACF++  +  +  GP VP I+L+L ++  + W+I G N+M++     +CL FVDGG++P+
Sbjct: 338 ACFDANRIPTSKMGPTVPSIDLVLDNKKNIMWRIFGANAMIQPRPGVMCLAFVDGGMRPK 397

Query: 388 NAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKKSCSEFS 408
             +V+G  Q+ED +L FD+  S LGFSSSLL R+ +C+ F+
Sbjct: 398 APIVIGTQQLEDNLLQFDLMNSRLGFSSSLLFRRTNCANFN 421

BLAST of Carg05448 vs. ExPASy Swiss-Prot
Match: Q9FSH9 (Gamma conglutin 1 OS=Lupinus albus OX=3870 GN=Cgamma PE=1 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 2.5e-45
Identity = 135/417 (32.37%), Postives = 203/417 (48.68%), Query Frame = 0

Query: 28  LVIPVNKDSVTNQYLATLYHGSPIKPVHLAVDLGGRFLWMACG---GSSSSRPILSRSIQ 87
           LV+P+ +D+ T  +   +   +P+  V + +DL G+ LW+ C     SS+ +     S Q
Sbjct: 48  LVLPIQQDASTKLHWGNILKRTPLMQVPVLLDLNGKHLWVTCSQHYSSSTYQAPFCHSTQ 107

Query: 88  CIAA----------VGGGRSGSSGSACDVIAGNPFGDLEGKAVLVEDTVAVRSLDRSTAA 147
           C  A              R G   + C +I+ NP     G   L +D +A+ S   S   
Sbjct: 108 CSRANTHQCFTCTDSTTSRPGCHNNTCGLISSNPVTQESGLGELAQDVLALHSTHGSKLG 167

Query: 148 VIVA----LHSCAPRFLLQ-GLAKSAKGVLGLGRNQISLPAQIATELGFHRRFSVCLSS- 207
            +V     L SCAP FL Q GL  + +G LGLG   ISLP Q+ +  G  R+F++CLSS 
Sbjct: 168 SLVKIPQFLFSCAPTFLTQKGLPNNVQGALGLGHAPISLPNQLFSHFGLKRQFTMCLSSY 227

Query: 208 --TNGVV----FPDSGNLDSVYGS-EISSSLTYTPILTKKIDASQSPEYFINVKAIKVDG 267
             +NG +      D  N + ++ S ++   + YTP     +  S+  EYFI V AI+V+ 
Sbjct: 228 PTSNGAILFGDINDPNNNNYIHNSLDVLHDMVYTP-----LTISKQGEYFIQVSAIRVNK 287

Query: 268 NRL--DLNKSLLDLDGRSG---GGGGGGTRLSTVVPYTTLESSIFNSLTAAFRAAAAAMN 327
           + +    N S+      S        GG  ++T  PYT L  SIF   T  F  A     
Sbjct: 288 HMVIPTKNPSMFPSSSSSSYHESSEIGGAMITTTNPYTVLRHSIFEVFTQVF--ANNVPK 347

Query: 328 MKEVAPVAPFEACFESENMEMTATGPKVPEIELILQSEMVGWKINGRNSMVKVNDETLCL 387
             +V  V PF  C++++ +        VP ++LI+    V W+I+G N MV+  D   CL
Sbjct: 348 QAQVKAVGPFGLCYDTKKI-----SGGVPSVDLIMDKSDVVWRISGENLMVQAQDGVSCL 407

Query: 388 GFVDGGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGF-SSSLLQRKKSCSEFSPENLP 413
           GFVDGG+  R  + LG +Q+E+ ++ FD+  S +GF ++SL    KSCS     N P
Sbjct: 408 GFVDGGVHTRAGIALGTHQLEENLVVFDLARSRVGFNTNSLKSHGKSCSNLFDLNNP 452

BLAST of Carg05448 vs. ExPASy Swiss-Prot
Match: Q42369 (Gamma conglutin 1 OS=Lupinus angustifolius OX=3871 GN=LOC109345795 PE=1 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 5.5e-45
Identity = 138/415 (33.25%), Postives = 203/415 (48.92%), Query Frame = 0

Query: 28  LVIPVNKDSVTNQYLATLYHGSPIKPVHLAVDLGGRFLWMACG---GSSSSRPILSRSIQ 87
           LV+PV +D+ T  + A ++  +P+  V L +DL G+ LW+ C     SS+ +     S Q
Sbjct: 47  LVLPVQEDASTGLHWANIHKRTPLMQVPLLLDLNGKHLWVTCSQHYSSSTYQAPFCHSTQ 106

Query: 88  CIAA----------VGGGRSGSSGSACDVIAGNPFGDLEGKAVLVEDTVAVRSLDRSTAA 147
           C  A              R G   + C +++ NP     G   L +D +A+ S   S   
Sbjct: 107 CSRANTHQCFTCTDSTTTRPGCHNNTCGLLSSNPVTQESGLGELAQDVLAIHSTHGSKLG 166

Query: 148 VIVA----LHSCAPRFLLQ-GLAKSAKGVLGLGRNQISLPAQIATELGFHRRFSVCL--- 207
            +V     L SCAP FL Q GL  + +G LGLG+  ISL  Q+ +  G  R+FSVCL   
Sbjct: 167 PMVKVPQFLFSCAPSFLAQKGLPNNVQGALGLGQAPISLQNQLFSHFGLKRQFSVCLSRY 226

Query: 208 SSTNGVV----FPDSGNLDSVYGS-EISSSLTYTPILTKKIDASQSPEYFINVKAIKVDG 267
           S++NG +      D  N + ++ S ++   L YTP     +  S+  EYFI V AI+V+ 
Sbjct: 227 STSNGAILFGDINDPNNNNYIHNSLDVLHDLVYTP-----LTISKQGEYFIQVNAIRVNK 286

Query: 268 NRLDLNKS-LLDLDGRSGGGGG--GGTRLSTVVPYTTLESSIFNSLTAAFRAAAAAMNMK 327
           + +   K+  +     S  G G  GG  ++T  PYT L  SIF   T  F  A       
Sbjct: 287 HLVIPTKNPFISPSSTSYHGSGEIGGALITTTHPYTVLSHSIFEVFTQVF--ANNMPKQA 346

Query: 328 EVAPVAPFEACFESENMEMTATGPKVPEIELILQSEMVGWKINGRNSMVKVNDETLCLGF 387
           +V  V PF  C++S  +   A     P ++LIL      W+I+  N MV+  D   CLGF
Sbjct: 347 QVKAVGPFGLCYDSRKISGGA-----PSVDLILDKNDAVWRISSENFMVQAQDGVSCLGF 406

Query: 388 VDGGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGF-SSSLLQRKKSCSEFSPENLP 413
           VDGG+  R  + LG + +E+ ++ FD+  S +GF S+SL    K+CS     N P
Sbjct: 407 VDGGVHARAGIALGAHHLEENLVVFDLERSRVGFNSNSLKSYGKTCSNLFDLNNP 449

BLAST of Carg05448 vs. ExPASy TrEMBL
Match: A0A6J1H4R8 (basic 7S globulin-like OS=Cucurbita moschata OX=3662 GN=LOC111460386 PE=4 SV=1)

HSP 1 Score: 783.1 bits (2021), Expect = 5.8e-223
Identity = 409/417 (98.08%), Postives = 411/417 (98.56%), Query Frame = 0

Query: 1   MAVPESRLLLLL-LLLCFSALFHGGAFSLVIPVNKDSVTNQYLATLYHGSPIKPVHLAVD 60
           MAVPESRLLLLL LLLCFSALFHGGAFSLVIPVNKDSVTNQYLATLYHGSPIKPVHLAVD
Sbjct: 1   MAVPESRLLLLLRLLLCFSALFHGGAFSLVIPVNKDSVTNQYLATLYHGSPIKPVHLAVD 60

Query: 61  LGGRFLWMACGGSSSSRPILSRSIQCIAAVGGGRSGSSGSACDVIAGNPFGDLEGKAVLV 120
           LGG+FLWMACGGSSSSRPILSRSIQCIAAVGGGRSGSSGSACDVIAGNPFGDLEGKAVLV
Sbjct: 61  LGGQFLWMACGGSSSSRPILSRSIQCIAAVGGGRSGSSGSACDVIAGNPFGDLEGKAVLV 120

Query: 121 EDTVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSAKGVLGLGRNQISLPAQIATELGFH 180
           EDTVAVRSLDRSTAAVIVALHSCAPRFLLQGLA+SAKGVLGLGRNQISLPAQIATELGFH
Sbjct: 121 EDTVAVRSLDRSTAAVIVALHSCAPRFLLQGLAESAKGVLGLGRNQISLPAQIATELGFH 180

Query: 181 RRFSVCLSSTNGVVFPDSGNLDSVYGSEISSSLTYTPILTKKIDASQSPEYFINVKAIKV 240
           RRFSVCLSSTNGVVFPDSGNLDSVYGSEISSSLTYTPILTKKIDASQSPEYFINVKAIKV
Sbjct: 181 RRFSVCLSSTNGVVFPDSGNLDSVYGSEISSSLTYTPILTKKIDASQSPEYFINVKAIKV 240

Query: 241 DGNRLDLNKSLLDLDGRSGGGGGGGTRLSTVVPYTTLESSIFNSLTAAFRAAAAAMNMKE 300
           DGNRLDLNKSLLDLD    GG GGGTRLSTVVPYTTLESSIFNSLTAAFRAAAAAMNMKE
Sbjct: 241 DGNRLDLNKSLLDLD----GGSGGGTRLSTVVPYTTLESSIFNSLTAAFRAAAAAMNMKE 300

Query: 301 VAPVAPFEACFESENMEMTATGPKVPEIELILQSEMVGWKINGRNSMVKVNDETLCLGFV 360
           VAPVAPFEACFESENMEMTATGPKVPEIELILQSEMVGWKINGRNSMVKVNDETLCLGFV
Sbjct: 301 VAPVAPFEACFESENMEMTATGPKVPEIELILQSEMVGWKINGRNSMVKVNDETLCLGFV 360

Query: 361 DGGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKKSCSEFSPENLPIRAG 417
           DGGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKKSCSEFSPENLPIRAG
Sbjct: 361 DGGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKKSCSEFSPENLPIRAG 413

BLAST of Carg05448 vs. ExPASy TrEMBL
Match: A0A6J1L1S1 (basic 7S globulin-like OS=Cucurbita maxima OX=3661 GN=LOC111499071 PE=4 SV=1)

HSP 1 Score: 775.8 bits (2002), Expect = 9.2e-221
Identity = 405/416 (97.36%), Postives = 407/416 (97.84%), Query Frame = 0

Query: 1   MAVPESRLLLLLLLLCFSALFHGGAFSLVIPVNKDSVTNQYLATLYHGSPIKPVHLAVDL 60
           MAVP+SR  LLLLLLCFSALFHGGAFSLVIPVNKDSVTNQYLATLYHGS IKPVHLAVDL
Sbjct: 1   MAVPKSR-FLLLLLLCFSALFHGGAFSLVIPVNKDSVTNQYLATLYHGSQIKPVHLAVDL 60

Query: 61  GGRFLWMACGGSSSSRPILSRSIQCIAAVGGGRSGSSGSACDVIAGNPFGDLEGKAVLVE 120
           GGRFLWMACGGSSSSRPILSRSIQCIAAVGGGRSGSSGSACDVIAGNPFGDLEGKAVLVE
Sbjct: 61  GGRFLWMACGGSSSSRPILSRSIQCIAAVGGGRSGSSGSACDVIAGNPFGDLEGKAVLVE 120

Query: 121 DTVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSAKGVLGLGRNQISLPAQIATELGFHR 180
           DTVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSAKGVLGLGRNQISLPAQIATELGFHR
Sbjct: 121 DTVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSAKGVLGLGRNQISLPAQIATELGFHR 180

Query: 181 RFSVCLSSTNGVVFPDSGNLDSVYGSEISSSLTYTPILTKKIDASQSPEYFINVKAIKVD 240
           RFSVCLSSTNGVVFPD+GNLDSVYGSEISSSLTYTPILTKK DASQSPEYFINVKAIKVD
Sbjct: 181 RFSVCLSSTNGVVFPDTGNLDSVYGSEISSSLTYTPILTKKTDASQSPEYFINVKAIKVD 240

Query: 241 GNRLDLNKSLLDLDGRSGGGGGGGTRLSTVVPYTTLESSIFNSLTAAFRAAAAAMNMKEV 300
           GNRLDLNKSLLDLD    GGGGGGTRLSTVVPYTTL SSIFNSLTAAFRAAAAAMNMKEV
Sbjct: 241 GNRLDLNKSLLDLD----GGGGGGTRLSTVVPYTTLGSSIFNSLTAAFRAAAAAMNMKEV 300

Query: 301 APVAPFEACFESENMEMTATGPKVPEIELILQSEMVGWKINGRNSMVKVNDETLCLGFVD 360
           APVAPFEACFESENMEMTATGPKVPEIELILQSEMVGWKINGRNSMVKVNDETLCLGFVD
Sbjct: 301 APVAPFEACFESENMEMTATGPKVPEIELILQSEMVGWKINGRNSMVKVNDETLCLGFVD 360

Query: 361 GGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKKSCSEFSPENLPIRAG 417
           GGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKKSCSEFSPENLPIRAG
Sbjct: 361 GGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKKSCSEFSPENLPIRAG 411

BLAST of Carg05448 vs. ExPASy TrEMBL
Match: A0A0A0KHJ8 (Basic 7S globulin 2 small subunit OS=Cucumis sativus OX=3659 GN=Csa_6G237600 PE=4 SV=1)

HSP 1 Score: 698.0 bits (1800), Expect = 2.4e-197
Identity = 365/411 (88.81%), Postives = 377/411 (91.73%), Query Frame = 0

Query: 1   MAVPESRLLLLLLLLCFSALFHGGAFSLVIPVNKDSVTNQYLATLYHGSPIKPVHLAVDL 60
           MAVP      LLLLL    LFHG  FSLVIP+ KDS+TNQY+AT++HGSPIKPVHLAVDL
Sbjct: 1   MAVP--HFPFLLLLLSSLTLFHGQTFSLVIPLTKDSLTNQYVATVFHGSPIKPVHLAVDL 60

Query: 61  GGRFLWMACGGSSSSRPILSRSIQCIAAVGGGRSGSSGSACDVIAGNPFGDLEGKAVLVE 120
           GG+ LWMACGGSSSSR I SRSIQCIAA GGGRSGS G ACDVIAGNPFGDLEGKA+LVE
Sbjct: 61  GGQSLWMACGGSSSSRSIPSRSIQCIAATGGGRSGSVGGACDVIAGNPFGDLEGKAILVE 120

Query: 121 DTVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSAKGVLGLGRNQISLPAQIATELGFHR 180
           DTVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKS KGVLGLGRNQISLPAQIATELG HR
Sbjct: 121 DTVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSVKGVLGLGRNQISLPAQIATELGSHR 180

Query: 181 RFSVCLSSTNGVVFPDSGNLDSVYGSEISSSLTYTPILTKKIDASQSPEYFINVKAIKVD 240
           RFS+CLSSTNGVVFPDSG+ DSVYGSEISSSLTYTPILTKKIDA QSPEYFINVKAIKVD
Sbjct: 181 RFSLCLSSTNGVVFPDSGSQDSVYGSEISSSLTYTPILTKKIDALQSPEYFINVKAIKVD 240

Query: 241 GNRLDLNKSLLDLDGRSGGGGGGGTRLSTVVPYTTLESSIFNSLTAAFRAAAAAMNMKEV 300
           GNRLDLNKSLLDL+G   G GGGGTRLSTVVPYT LESSIFNSLTAAFRAAAAAMNMKEV
Sbjct: 241 GNRLDLNKSLLDLEGVGDGEGGGGTRLSTVVPYTVLESSIFNSLTAAFRAAAAAMNMKEV 300

Query: 301 APVAPFEACFESENMEMTATGPKVPEIELILQSEMVGWKINGRNSMVKVNDETLCLGFVD 360
           APVAPFE CFESENMEMTA GPKVPEIELILQSEMVGWKI GRNSMVKVNDE  CLGFVD
Sbjct: 301 APVAPFEVCFESENMEMTAAGPKVPEIELILQSEMVGWKIYGRNSMVKVNDEAYCLGFVD 360

Query: 361 GGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKKSCSEFSPENL 412
           GGLKPRNA+VLGGYQMEDIVLDFDMGTSMLGFSSSLLQRK+SCSEFSP NL
Sbjct: 361 GGLKPRNAIVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKRSCSEFSPVNL 409

BLAST of Carg05448 vs. ExPASy TrEMBL
Match: A0A5A7SQG3 (Basic 7S globulin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold111G00740 PE=4 SV=1)

HSP 1 Score: 696.4 bits (1796), Expect = 7.1e-197
Identity = 366/417 (87.77%), Postives = 379/417 (90.89%), Query Frame = 0

Query: 1   MAVPESRLLLLLLLLCFS-ALFHGGAFSLVIPVNKDSVTNQYLATLYHGSPIKPVHLAVD 60
           MAVP S  LLLLLLL  S  LFHG  FSLVIP+ KDS+TNQYLAT++HGSPIKPVHLAVD
Sbjct: 1   MAVPHSPFLLLLLLLLSSLTLFHGQTFSLVIPLTKDSLTNQYLATVFHGSPIKPVHLAVD 60

Query: 61  LGGRFLWMACGGSSSSRPILSRSIQCIAAVGGGRSGSSGSACDVIAGNPFGDLEGKAVLV 120
           LGGR LWMACGGSSSSR I SRSIQCIAA  GGRSGS G AC+VIA NPFGDLEGKA+LV
Sbjct: 61  LGGRSLWMACGGSSSSRSIPSRSIQCIAATAGGRSGSVGGACNVIAENPFGDLEGKAILV 120

Query: 121 EDTVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSAKGVLGLGRNQISLPAQIATELGFH 180
           EDTVAV SL RSTAAVIVALHSCAPRFLLQGLAKS KGVLGLGRNQISLPAQIATELG H
Sbjct: 121 EDTVAVGSLGRSTAAVIVALHSCAPRFLLQGLAKSVKGVLGLGRNQISLPAQIATELGSH 180

Query: 181 RRFSVCLSSTNGVVFPDSGNLDSVYGSEISSSLTYTPILTKKIDASQSPEYFINVKAIKV 240
           RRFS+CLSSTNGVVFPDSG+ DSVYGSEISSSLTYTPILTKKIDA  SPEYFINVKAIKV
Sbjct: 181 RRFSLCLSSTNGVVFPDSGSPDSVYGSEISSSLTYTPILTKKIDALLSPEYFINVKAIKV 240

Query: 241 DGNRLDLNKSLLDLDGRSGGGGGGGTRLSTVVPYTTLESSIFNSLTAAFRAAAAAMNMKE 300
           DGNRLDLNKSLLDL+    GGGGGGTRLSTVVPYT LESSIFNSLT AFRAAAAAMNMKE
Sbjct: 241 DGNRLDLNKSLLDLEEAGDGGGGGGTRLSTVVPYTALESSIFNSLTVAFRAAAAAMNMKE 300

Query: 301 VAPVAPFEACFESENMEMTATGPKVPEIELILQSEMVGWKINGRNSMVKVNDETLCLGFV 360
           VAPVAPFEACFESENMEMTA GPKVPEIELILQSEMVGWKI GRNSMVKVND+  CLGFV
Sbjct: 301 VAPVAPFEACFESENMEMTAAGPKVPEIELILQSEMVGWKIYGRNSMVKVNDKAYCLGFV 360

Query: 361 DGGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKKSCSEFSPENLPIRAG 417
           DGGLKPRNA+VLGGYQMEDIVLDFD+GTSMLGFSSSLLQRK+SCSEFSPENL  R G
Sbjct: 361 DGGLKPRNAIVLGGYQMEDIVLDFDIGTSMLGFSSSLLQRKRSCSEFSPENLLKRTG 417

BLAST of Carg05448 vs. ExPASy TrEMBL
Match: A0A1S3C7N9 (basic 7S globulin-like OS=Cucumis melo OX=3656 GN=LOC103497928 PE=4 SV=1)

HSP 1 Score: 696.4 bits (1796), Expect = 7.1e-197
Identity = 366/417 (87.77%), Postives = 379/417 (90.89%), Query Frame = 0

Query: 1   MAVPESRLLLLLLLLCFS-ALFHGGAFSLVIPVNKDSVTNQYLATLYHGSPIKPVHLAVD 60
           MAVP S  LLLLLLL  S  LFHG  FSLVIP+ KDS+TNQYLAT++HGSPIKPVHLAVD
Sbjct: 1   MAVPHSPFLLLLLLLLSSLTLFHGQTFSLVIPLTKDSLTNQYLATVFHGSPIKPVHLAVD 60

Query: 61  LGGRFLWMACGGSSSSRPILSRSIQCIAAVGGGRSGSSGSACDVIAGNPFGDLEGKAVLV 120
           LGGR LWMACGGSSSSR I SRSIQCIAA  GGRSGS G AC+VIA NPFGDLEGKA+LV
Sbjct: 61  LGGRSLWMACGGSSSSRSIPSRSIQCIAATAGGRSGSVGGACNVIAENPFGDLEGKAILV 120

Query: 121 EDTVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSAKGVLGLGRNQISLPAQIATELGFH 180
           EDTVAV SL RSTAAVIVALHSCAPRFLLQGLAKS KGVLGLGRNQISLPAQIATELG H
Sbjct: 121 EDTVAVGSLGRSTAAVIVALHSCAPRFLLQGLAKSVKGVLGLGRNQISLPAQIATELGSH 180

Query: 181 RRFSVCLSSTNGVVFPDSGNLDSVYGSEISSSLTYTPILTKKIDASQSPEYFINVKAIKV 240
           RRFS+CLSSTNGVVFPDSG+ DSVYGSEISSSLTYTPILTKKIDA  SPEYFINVKAIKV
Sbjct: 181 RRFSLCLSSTNGVVFPDSGSPDSVYGSEISSSLTYTPILTKKIDALLSPEYFINVKAIKV 240

Query: 241 DGNRLDLNKSLLDLDGRSGGGGGGGTRLSTVVPYTTLESSIFNSLTAAFRAAAAAMNMKE 300
           DGNRLDLNKSLLDL+    GGGGGGTRLSTVVPYT LESSIFNSLT AFRAAAAAMNMKE
Sbjct: 241 DGNRLDLNKSLLDLEEAGDGGGGGGTRLSTVVPYTALESSIFNSLTVAFRAAAAAMNMKE 300

Query: 301 VAPVAPFEACFESENMEMTATGPKVPEIELILQSEMVGWKINGRNSMVKVNDETLCLGFV 360
           VAPVAPFEACFESENMEMTA GPKVPEIELILQSEMVGWKI GRNSMVKVND+  CLGFV
Sbjct: 301 VAPVAPFEACFESENMEMTAAGPKVPEIELILQSEMVGWKIYGRNSMVKVNDKAYCLGFV 360

Query: 361 DGGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKKSCSEFSPENLPIRAG 417
           DGGLKPRNA+VLGGYQMEDIVLDFD+GTSMLGFSSSLLQRK+SCSEFSPENL  R G
Sbjct: 361 DGGLKPRNAIVLGGYQMEDIVLDFDIGTSMLGFSSSLLQRKRSCSEFSPENLLKRTG 417

BLAST of Carg05448 vs. TAIR 10
Match: AT1G03220.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 269.2 bits (687), Expect = 5.4e-72
Identity = 168/424 (39.62%), Postives = 243/424 (57.31%), Query Frame = 0

Query: 6   SRLLLLLLLLCFSALFHGGAFSLVIPVNKDSVTNQYLATLYHGSPIKPVHLAVDLGGRFL 65
           S LLL +  L  SA       +L++PV KD  T QY   +   +P+ P  +  DLGGR L
Sbjct: 9   SVLLLFIFSLSSSAQTPFRPKALLLPVTKDQSTLQYTTVINQRTPLVPASVVFDLGGREL 68

Query: 66  WMAC--GGSSS-------SRPILSR--SIQCIAAVGGGRSGSSGSACDVIAGNPFGDLEG 125
           W+ C  G  SS       +  + SR  S  C       R G S + C  I  N       
Sbjct: 69  WVDCDKGYVSSTYQSPRCNSAVCSRAGSTSCGTCFSPPRPGCSNNTCGGIPDNTVTGTAT 128

Query: 126 KAVLVEDTVAVRSLDRSTAAVIV----ALHSCAPRFLLQGLAKSAKGVLGLGRNQISLPA 185
                 D V+++S + S    +V     +  C   FLL+GLAK   G+ G+GR+ I LP+
Sbjct: 129 SGEFALDVVSIQSTNGSNPGRVVKIPNLIFDCGATFLLKGLAKGTVGMAGMGRHNIGLPS 188

Query: 186 QIATELGFHRRFSVCLSSTNGVVFPDSGNLDSVYGSEISSSLTYTPILTKKIDAS----- 245
           Q A    FHR+F+VCL+S  GV F  +G    + G +I SSL  TP+L   +  +     
Sbjct: 189 QFAAAFSFHRKFAVCLTSGKGVAFFGNGPYVFLPGIQI-SSLQTTPLLINPVSTASAFSQ 248

Query: 246 --QSPEYFINVKAIKVDGNRLDLNKSLLDLDGRSGGGGGGGTRLSTVVPYTTLESSIFNS 305
             +S EYFI V AI++    + +N +LL ++  +   G GGT++S+V PYT LESSI+N+
Sbjct: 249 GEKSSEYFIGVTAIQIVEKTVPINPTLLKINAST---GIGGTKISSVNPYTVLESSIYNA 308

Query: 306 LTAAFRAAAAAMNMKEVAPVAPFEACFESENMEMTATGPKVPEIELILQSEMVGWKINGR 365
            T+ F   AAA ++K VA V PF ACF ++N+ +T  G  VPEIEL+L S+ V W+I G 
Sbjct: 309 FTSEFVKQAAARSIKRVASVKPFGACFSTKNVGVTRLGYAVPEIELVLHSKDVVWRIFGA 368

Query: 366 NSMVKVNDETLCLGFVDGGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKKSC 408
           NSMV V+D+ +CLGFVDGG+  R +VV+GG+Q+ED +++FD+ ++  GFSS+LL R+ +C
Sbjct: 369 NSMVSVSDDVICLGFVDGGVNARTSVVIGGFQLEDNLIEFDLASNKFGFSSTLLGRQTNC 428

BLAST of Carg05448 vs. TAIR 10
Match: AT1G03230.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 257.3 bits (656), Expect = 2.1e-68
Identity = 163/424 (38.44%), Postives = 235/424 (55.42%), Query Frame = 0

Query: 6   SRLLLLLLLLCFSALFHGGAFSLVIPVNKDSVTNQYLATLYHGSPIKPVHLAVDLGGRFL 65
           S LLL +  L  SA       +L++PV KD  T QY   +   +P+ P  +  DLGGR  
Sbjct: 10  SVLLLSIFSLSSSAQPSFRPKALLLPVTKDPSTLQYTTVINQRTPLVPASVVFDLGGREF 69

Query: 66  WMACG---------GSSSSRPILSR--SIQCIAAVGGGRSGSSGSACDVIAGNPFGDLEG 125
           W+ C              +  + SR  SI C       R G S + C     N       
Sbjct: 70  WVDCDQGYVSTTYRSPRCNSAVCSRAGSIACGTCFSPPRPGCSNNTCGAFPDNSITGWAT 129

Query: 126 KAVLVEDTVAVRSLDRSTAAVIV----ALHSCAPRFLLQGLAKSAKGVLGLGRNQISLPA 185
                 D V+++S + S     V     + SC    LL+GLAK A G+ G+GR+ I LP 
Sbjct: 130 SGEFALDVVSIQSTNGSNPGRFVKIPNLIFSCGSTSLLKGLAKGAVGMAGMGRHNIGLPL 189

Query: 186 QIATELGFHRRFSVCLSSTNGVVFPDSGNLDSVYGSEISSSLTYTPILTKK-------ID 245
           Q A    F+R+F+VCL+S  GV F  +G    + G +I S L  TP+L            
Sbjct: 190 QFAAAFSFNRKFAVCLTSGRGVAFFGNGPYVFLPGIQI-SRLQKTPLLINPGTTVFEFSK 249

Query: 246 ASQSPEYFINVKAIKVDGNRLDLNKSLLDLDGRSGGGGGGGTRLSTVVPYTTLESSIFNS 305
             +SPEYFI V AIK+    L ++ +LL ++  +   G GGT++S+V PYT LESSI+ +
Sbjct: 250 GEKSPEYFIGVTAIKIVEKTLPIDPTLLKINAST---GIGGTKISSVNPYTVLESSIYKA 309

Query: 306 LTAAFRAAAAAMNMKEVAPVAPFEACFESENMEMTATGPKVPEIELILQSEMVGWKINGR 365
            T+ F   AAA ++K VA V PF ACF ++N+ +T  G  VPEI+L+L S+ V W+I G 
Sbjct: 310 FTSEFIRQAAARSIKRVASVKPFGACFSTKNVGVTRLGYAVPEIQLVLHSKDVVWRIFGA 369

Query: 366 NSMVKVNDETLCLGFVDGGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKKSC 408
           NSMV V+D+ +CLGFVDGG+ P  +VV+GG+Q+ED +++FD+ ++  GFSS+LL R+ +C
Sbjct: 370 NSMVSVSDDVICLGFVDGGVNPGASVVIGGFQLEDNLIEFDLASNKFGFSSTLLGRQTNC 429

BLAST of Carg05448 vs. TAIR 10
Match: AT5G19120.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 234.6 bits (597), Expect = 1.5e-61
Identity = 160/388 (41.24%), Postives = 211/388 (54.38%), Query Frame = 0

Query: 28  LVIPVNKDSVTNQYLATLYHGSPIKPVHLAVDLGGRFLWMACGGS--SSSRPILSRSIQ- 87
           +V PV KD  T QYLA +  G    PV L VDL G  LW  C     SSSR ++S S   
Sbjct: 32  VVFPVVKDLPTGQYLAQIRLGDSPDPVKLVVDLAGSILWFDCSSRHVSSSRNLISGSSSG 91

Query: 88  CI-AAVGGGRSGSSGSA-------CDVIAGNPFGDLEGKAVLVEDTVAVRSLDRSTAAVI 147
           C+ A VG  R  SS S+       C+++  N    +  +  L  D ++V S+  ++   +
Sbjct: 92  CLKAKVGNERVSSSSSSRKDQNADCELLVKNDAFGITARGELFSDVMSVGSV--TSPGTV 151

Query: 148 VALHSCAPRFLLQGLAKSAKGVLGLGRNQISLPAQIATELGFHRRFSVCLSSTNGVVFPD 207
             L +C P +LL+GLA  A+GV+GLGR QISLP+Q+A E    RR +V LS  NGVV   
Sbjct: 152 DLLFACTPPWLLRGLASGAQGVMGLGRAQISLPSQLAAETNERRRLTVYLSPLNGVV--S 211

Query: 208 SGNLDSVYGSEISSSLTYTPILTKKIDASQSPEYFINVKAIKVDGNRLDLNKSLLDLDGR 267
           + +++ V+G   S SL YTP+LT       S  Y INVK+I+V+G +L +   L      
Sbjct: 212 TSSVEEVFGVAASRSLVYTPLLT-----GSSGNYVINVKSIRVNGEKLSVEGPL------ 271

Query: 268 SGGGGGGGTRLSTVVPYTTLESSIFNSLTAAFRAAAAAMNMKEVAPVAPFEACFESENME 327
                     LSTVVPYT LESSI+     A+  A AA     V PVAPF  CF S+   
Sbjct: 272 -------AVELSTVVPYTILESSIYKVFAEAY--AKAAGEATSVPPVAPFGLCFTSD--- 331

Query: 328 MTATGPKVPEIELILQSEMVGWKINGRNSMVKVNDETLCLGFVDGGLKPRNAVVLGGYQM 387
                   P ++L LQSEMV W+I+G+N MV V     C G VDGG    N +V+GG Q+
Sbjct: 332 -----VDFPAVDLALQSEMVRWRIHGKNLMVDVGGGVRCSGIVDGGSSRVNPIVMGGLQL 383

Query: 388 EDIVLDFDMGTSMLGFSSSLLQRKKSCS 405
           E  +LDFD+G SM+GF     QR +S S
Sbjct: 392 EGFILDFDLGNSMMGFG----QRTRSDS 383

BLAST of Carg05448 vs. TAIR 10
Match: AT5G19110.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 183.3 bits (464), Expect = 3.9e-46
Identity = 139/409 (33.99%), Postives = 202/409 (49.39%), Query Frame = 0

Query: 10  LLLLLLCFSALFHGGAFSLVIPVNKDSVTNQYLATLYHGSPIK-PVHLAVDLGGRFLWMA 69
           LL+ L  F+A+        ++P+ K   TN +  T   GS  K PV+L +DLG    W+ 
Sbjct: 8   LLVFLSIFAAIALKSNSQYLLPITKHEPTNLFYTTFNVGSAAKSPVNLLLDLGTNLTWLD 67

Query: 70  C---GGSSSSRPILSRSIQCIAAVGGGRSGSSGSACDVIAGNPFGD---LEGKAVLVEDT 129
           C      SS R +  +S  C +  G   +G +G +C     NP G    + G+  +V+D 
Sbjct: 68  CRKLKSLSSLRLVTCQSSTCKSIPG---NGCAGKSCLYKQPNPLGQNPVVTGR--VVQDR 127

Query: 130 VAVRSLD----RSTAAVIVALHSCAPRFLLQGLAKSAKGVLGLGRNQISLPAQIATELGF 189
            ++ + D     S  +V     SCA    LQGL     GVL L     S   Q+ +    
Sbjct: 128 ASLYTTDGGKFLSQVSVRHFTFSCAGEKALQGLPPPVDGVLALSPGSSSFTKQVTSAFNV 187

Query: 190 HRRFSVCLSSTNGVVFPDSGNLDSVYGSEISSSLTYTPILTKKIDASQSPEYFINVKAIK 249
             +FS+CL S+    F  +G     +    +SS    P     I  + S +Y I VK+I 
Sbjct: 188 IPKFSLCLPSSGTGHFYIAG--IHYFIPPFNSSDNPIPRTLTPIKGTDSGDYLITVKSIY 247

Query: 250 VDGNRLDLNKSLLDLDGRSGGGGGGGTRLSTVVPYTTLESSIFNSLTAAFRAAAAAMNMK 309
           V G  L LN  LL           GG +LSTVV YT L++ I+N+L  +F   A AM + 
Sbjct: 248 VGGTALKLNPDLLT----------GGAKLSTVVHYTVLQTDIYNALAQSFTLKAKAMGIA 307

Query: 310 EVAPVAPFEACFESENMEMTAT-GPKVPEIELILQSEM--VGWKINGRNSMVKVNDETLC 369
           +V  VAPF+ CF+S       T GP VP IE+ L   +  V W   G N++VKV +  +C
Sbjct: 308 KVPSVAPFKHCFDSRTAGKNLTAGPNVPVIEIGLPGRIGEVKWGFYGANTVVKVKETVMC 367

Query: 370 LGFVDGGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKKSCS 405
           L F+DGG  P++ +V+G +Q++D +L+FD   ++L FS SLL    SCS
Sbjct: 368 LAFIDGGKTPKDLMVIGTHQLQDHMLEFDFSGTVLAFSESLLLHNTSCS 399

BLAST of Carg05448 vs. TAIR 10
Match: AT5G19100.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 132.5 bits (332), Expect = 7.9e-31
Identity = 124/417 (29.74%), Postives = 188/417 (45.08%), Query Frame = 0

Query: 7   RLLLLLLLLCFSALFHGGAF-----SLVIPVNKDSVTNQYLATLYHGSPIKPVHLAVDLG 66
           R++ LLL L F  L +         S + P+ KD+  N Y   L  GS        +DL 
Sbjct: 4   RVIFLLLSLVFLYLANTSHSLRKFQSFLHPIYKDTAKNIYTIPLSIGS-TSSEKFVLDLN 63

Query: 67  GRF-LWMAC---GGSSSSRPILSRSIQCIAAVGGGRSGSSGSAC--DVIAGNPFGDLEG- 126
           G   L   C     S++  PI   S +C       +  +    C  +VIA      L   
Sbjct: 64  GAAPLLQNCPTAAKSTTYHPIRCGSTRC-------KYANPNFPCPNNVIAKKRTVCLSSD 123

Query: 127 KAVLVEDTVAVRSLDRSTAAVIVALHSCAPRFLLQGLAKSAKGVLGLGRNQISLPAQIAT 186
            + L  DTV +             + S        G     +  +GL    +S+P+Q+ +
Sbjct: 124 NSRLFRDTVPLLYTFNGVYTRDSEMSSSLTLTCTDGAPALKQRTIGLANTHLSIPSQLIS 183

Query: 187 ELGFHRRFSVCLSST------NGVVFPDSGNLDSV-YGSEISSSLTYTPILTKKIDASQS 246
                 + ++CL ST      NG ++   G    + Y  ++S     TP+    I   +S
Sbjct: 184 MYQLPHKIALCLPSTERSQSHNGDLWIGKGEYYYLPYDKDVSKIFASTPL----IGNGKS 243

Query: 247 PEYFINVKAIKVDGNRLDLNKSLLDLDGRSGGGGGGGTRLSTVVPYTTLESSIFNSLTAA 306
            EY I+VK+I++    + +                G T++ST+ PYT  ++S++ +L  A
Sbjct: 244 GEYLIDVKSIQIGAKTVPIPY--------------GATKISTLAPYTVFQTSLYKALLTA 303

Query: 307 FRAAAAAMNMKEVAPVAPFEACFESENMEMTATGPKVPEIELILQSEMVGWKINGRNSMV 366
           F      + + +   V PF ACF S        G  VP I+L+L S    W+I G NS+V
Sbjct: 304 F---TENIKIAKAPAVKPFGACFYSNG------GRGVPVIDLVL-SGGAKWRIYGSNSLV 363

Query: 367 KVNDETLCLGFVDGGLKPRNAVVLGGYQMEDIVLDFDMGTSMLGFSSSLLQRKKSCS 405
           KVN   +CLGFVDGG+KP+  +V+GG+QMED +++FD+  S   FSSSLL    SCS
Sbjct: 364 KVNKNVVCLGFVDGGVKPKYPIVIGGFQMEDNLVEFDLEASKFSFSSSLLLHNTSCS 384

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7013583.11.0e-229100.00Basic 7S globulin, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6575012.11.4e-22398.08putative aspartic proteinase GIP2, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022959401.11.2e-22298.08basic 7S globulin-like [Cucurbita moschata][more]
XP_023006289.11.9e-22097.36basic 7S globulin-like [Cucurbita maxima][more]
XP_023549177.14.2e-22097.12basic 7S globulin-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
P0DO211.6e-7641.28Probable aspartic proteinase GIP2 OS=Nicotiana benthamiana OX=4100 GN=GIP2 PE=1 ... [more]
I1JNS61.8e-5634.29Probable aspartic proteinase GIP1 OS=Glycine max OX=3847 GN=GIP1 PE=1 SV=2[more]
P829522.4e-5635.91Gamma conglutin 1 OS=Prunus dulcis OX=3755 GN=Cgamma1 PE=1 SV=2[more]
Q9FSH92.5e-4532.37Gamma conglutin 1 OS=Lupinus albus OX=3870 GN=Cgamma PE=1 SV=1[more]
Q423695.5e-4533.25Gamma conglutin 1 OS=Lupinus angustifolius OX=3871 GN=LOC109345795 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1H4R85.8e-22398.08basic 7S globulin-like OS=Cucurbita moschata OX=3662 GN=LOC111460386 PE=4 SV=1[more]
A0A6J1L1S19.2e-22197.36basic 7S globulin-like OS=Cucurbita maxima OX=3661 GN=LOC111499071 PE=4 SV=1[more]
A0A0A0KHJ82.4e-19788.81Basic 7S globulin 2 small subunit OS=Cucumis sativus OX=3659 GN=Csa_6G237600 PE=... [more]
A0A5A7SQG37.1e-19787.77Basic 7S globulin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1... [more]
A0A1S3C7N97.1e-19787.77basic 7S globulin-like OS=Cucumis melo OX=3656 GN=LOC103497928 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G03220.15.4e-7239.62Eukaryotic aspartyl protease family protein [more]
AT1G03230.12.1e-6838.44Eukaryotic aspartyl protease family protein [more]
AT5G19120.11.5e-6141.24Eukaryotic aspartyl protease family protein [more]
AT5G19110.13.9e-4633.99Eukaryotic aspartyl protease family protein [more]
AT5G19100.17.9e-3129.74Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 203..409
e-value: 3.4E-60
score: 205.0
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 25..195
e-value: 1.9E-32
score: 114.7
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 33..397
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 229..393
e-value: 5.8E-52
score: 175.7
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 41..190
e-value: 4.6E-26
score: 92.1
NoneNo IPR availablePANTHERPTHR47965:SF46BASIC 7S GLOBULIN 2 SMALL SUBUNITcoord: 8..406
IPR001461Aspartic peptidase A1 familyPANTHERPTHR47965ASPARTYL PROTEASE-RELATEDcoord: 8..406
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 41..393
score: 21.039131

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg05448-RACarg05448-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004190 aspartic-type endopeptidase activity