Carg05405 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg05405
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionPectate lyase
LocationCarg_Chr19: 937841 .. 943747 (-)
RNA-Seq ExpressionCarg05405
SyntenyCarg05405
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CATTCAATTCCCTCTCTTTCGCTTCACAACTACTCGCATTTAACGCTCTCATACAAATCCCACAGATCGAGCGATCGAGAGATCGAGAGATACAATACACAGTCGCTTAAAATTTTTTCGGAGAAGCAGAGAAAGCTTCAGAATCATCAATAATGGCGGTCACTCTTAGACTACAGTTTTCAACCGCTTCATTAATGCTCTTCCTTCTTCTCAACATCCTCTTCGTTCAATCCAGGTAAATGCTTCCTAATCGCGTCATTTCTCTGCGTTTTCGACAATAATGCCGTCGCGTCCGCCGCTACCGACGGCCTAAACTAGCGTAGTCTTGTTCTGAGTAATTTGTTGAATGTTGATCTTGATTTTTCTCATTTTCTGTTTCGTTTCACAATGTTGATAGTTTTGCTGGAGATCAGCGATTGCAGAGCTTTAATTCTTCATCCATGGCGGACCGGTCAGTTTCCGAAACTAGACCTTCTCTGTTCTGAGTAATTTTTTTAATGATTTTCTTGATTGTTCTCATTTTCTGTTTCGCTTCACAATGTTGATAGTTTTTCTGGAGATGAGCGACTGCAGAGCTTCAATTCTTCATCCATGGCGGACCGGTCAGTTTCCGAAAATAGACCTTCTCTGTTCTCTGTAATTTTCGAATGATTTTCTTGATTGTTCTCATTTTCTGTTTCGCTTCACAATGTTGATAGCTTTGCTGGAGATCAGCGATTGCAGAGCTTCAATTCTTCATCCATGGCGGACCGGTCAGTTTCCGAAACTAGACCTTCTCTGTTATTTTCCCCCCAATTTTTCTCTTCGTTTTCCGGCACATGATCTTCACGTGTCGATTTTATAAAAATATTCATAAAGTCTGAGAAAAATTATGGAAAACTAAATAATTTTACAATTTAAAATTAATTAAAATGCTAATTATTTTTATTATTATTAGTATTAAAGGCATTTTGTTACTTTTTTTTTGTGTTCCATAATTTTTTACAATATTGATAAAAATATTATAATGTAAATTTTATACTCTAGGTCGAAAGCGTAATTTAGGCCGTCAGATATATCAAACGGATCAGAAAATTGGAATTTTGAGATCGGATCAGGAATATCTGGGCCGTTCATTCGTAGATTAAAACAAAAAAAAGAGGTCACAGAGTGTCTCTATATTAAGTACTTTCTTTGACTCTCTATTGACAAATTTACCCTTCGACATTCCATTTCAACCCTGGCCTAATTTTGGACCTTTTGGTCAATGGCCATTTTCACTTCCACGTGTACATTTCTTTTTCTCAGATTTGAATCTAACTTTCAAGAGAACAAAAAAAAAAAAAAAAAAAGCTTTAAATACATAAATTAAATAATTCATTAAAACATACGGAACAGCCTTCCACCGACCAAGAGCTTAATAATAATAATATTATTATTTTGTTTCGATAATAAACTGAGATATTTTAATTATGTAATTAAATGTTAAATTAATTATTTATATAGATATTTGTTTAATTGAAAATTTCAGGTATGTTAAGAATGAGCATGCCGTTGATAATCCAGAGGAAATTGCATCAATGGTGGATTTGTAAGATTCCTCTCTCTCTCTCTAATATTTATTTATGGGGCCAAATGAATAATTAAAAATGATTATTAATAATAAAAATGGAATTTGCTTTCGATTAGCATGTGCCTTCTTTTACTCCAAGTATGCTGTTTTGATGCAAATCTGAAGTTTTAAATAAGTGTTAGAATGCGAAAATGACTTAAATAATAAGTCATTAATAGATTTAGTTTTTTTTTAATACTTAATTTATGAACTTTTAAGAGTCTATTTAATTTCGTGTAGTTGAATGTAACCGTTAAATTCGATAAAAAAAAATACATTTTATAATTATTAGTTTTATCTACTAAATTATAAAAAATATTTTTCAATTTTATCATTTGTTTAAAAAAAATTTATATACTATTTAAATACTATAATATTATTTTTGATATTTAATAAATATTTAAAAATTAGGAGTATAAATCCTTTATAAAATGTTGTGTTTGATATTCTAGAAGATTTTCCTTTTAAAATGTTTAAGAAAACTAAAAAATAATATTGTAATTTTTAAAAAATCAACCAGTTTAGAATTTAATTACAAGAGTGTTTTAGAAGATATTAGTGTTATAAAATTTATTTTATAATATGATTGGGAGGGACCCAGCCACCACGTTCATTGTCACTGCGATTTCAATCTCTTTCACACATGCCCATGTGGCGAGATCCACCTGTCACGCTTATAATTTAATTCTTGTCTCTTCGCTTATCCAAAAATATGGAAAATAGATATATAAATCTTCTTGAATTTATGTTGAAGTGAATAATATTAAGTCAATTTCTTTCGCTTTTAAAGGATTAATAAACTTATATTGTGAATCTTTCCTTTATACATTATGAGTTCCGCTAGACGTTTAAACTATAATTGAAGAGGAATATGATACATAAATGTCTGTATTTTACCTTTTTAAATCACTAACGACTATTTATGTTCTTGAATTTGGTTTCTTATTCGTTCGTTTATTTATGTTAATTTTTTTTTAAGAAGTTTCTAATAATTTCTTGAATTCTTGATTTTGTGTTTTATATGCAAAGTTGAATAACTTTTGTAGATGTGTTGATACTTTTTTTTTTTAAAAAAATTGTAATTTATTATTTTCTTTTTTCTGCTGGATTTATTGCGCAAATCATGCTGGCGGCAGCTGCATCGCATGCCGAAACTATAGTAATCAATAAATGAGACGACCTTAGCATTTAAAGATACCTTCACCGACACATCAAATTTTTGGTCAAATAAAGGAAACAAAAAATGGATTGCTTTCTGTTTCATCTGTAGCATTAAACTTTTACAGCCAACTTCACAACTTTTTAAGTAACGCTAGCTTTCCGTATGATCCGTATGCACCCAATTCATTGAATGTGGGACTCCACATTTAATGCCTATGAAATTCATTCTGGGTTTCACAAACAAAGCCTTAAATTTTCTTTCTTATGTTTGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATAGTATAAATTAGTTGAATTATGGTTGGATTAACCAATTTTCTTCTTTTAGGATTTAAACTTAATCGGTTGATGTTAAGAATAAGAAATAGAAGTGAGATTAAAAGTCTCACTTCTATTCTCATTCTTAATATGGTATAGAGCAACCAGTAGAGTTGGTGTCCATTTTTTTGTTGAGGATTGTTGGGGGGAGTTACACATTGGCAAACGATCATGAATTTATAAGTAACGAATACATCTCCAGTATGAGGCTTTTTGGGTAAGCCCAAAATAAAGACATGAGAGCTCTTATGCTCACAATATTATACCATTGTGGAGATCCGTGATGCTCTTAACTTAGTCATGTCAATAGAATCCAGGAGTCCACTTTGATTAATGGAAGAGAGGTTGTTCAAAAGCTCCATAGACCTCAGGAGAGGTTCTATAGTGTACTTTGTTCGAGGGTAGAGAGTTTGTTTGAGGGCTCTATGAGCCACAGGGGAAGTTATATAGTGTACTTTGTTTGAGGGAGAGTCCCAAATTGGCTAATTTAAGGAGTGGCTGAGCGATTCGCAGTTTGTTTCATGGCCTAAATTGGTTCATACTTACACATTTTTGTTTCATTCGATCTCACTTCTATTTTTCATTCTTTACGGTAGCACTTATAATGTGTATGTGTAGTGATTGGTTGGTTATGTTTGGAAATTTCCCACAGGAGCATACGCAACAGCACAGAGAGAAGGAACTTGGGGTTCTTCTCTTGTGGAACAGGAAATCCAATTGATGATTGTTGGCGTTGTGACCCACGTTGGCAACTGCGGCGAAAGCGCCTCGCCAATTGCGGCATTGGGTTTGGTCGTAATGCAGTCGGTGGCCGTGATGGGCGATACTACGTCGTTTCTGATCCACGTGACGACGATCCTGTCAATCCCAGACCAGGCACCCTCCGCCATGCTGTGATTCAAGACAGACCATTATGGATTGTGTTCAAACGAGACATGGTGATCACTTTAAAGCAAGAACTCATCATGAACAGCTTCAAGACAATTGATGGGCGTGGCGTCAATGTCCACATCGCTTATGGTGCTTGTATTACAATCCAATTCATTACCAATGTTATTATTCATGGTTTGCATATTCATGACTGCAAGCCAACGGGGAATGCCATGGTTCGAAGCTCGCCTAGTCATTATGGATGGAGGACAATGGCTGATGGTGATGGGATTTCAATTTTTGGGTCGAGTCATATTTGGATTGACCATAATTCTCTTTCTTCTTGTTCTGATGGGCTCATTGATGCTATCATGGGATCAACTGCTATCACAATTTCTAACAATTACTTCACCCACCACAATGAGGTAAAAAGTTAACAGTTTTGTCAAATGATCGCAAATGTTGGAATTTGTTCTGAAATTGGCTGAGATTGTTGCAAAACAGGTGATGCTCTTGGGACATAGTGACTCTTATGTAAGAGACAAGCAAATGCAAGTCACCATTGCTTATAACCATTTTGGTGAAGGTCTCATCCAAAGAATGCCAAGGTACTTTTCTCCTCTCGATTACCTTAGGAATCACGAACTCCTCCTTAATGGTATGATATTGTCTATTTTAAGCATAAACTCTCATGACTGAGTTGTTTTTGGTTTCCGAGAAGGTCTCGTACCAATCGAAATAGTGTTATTTACTTATAAAGCCATGATGTTCCTCTTGATTAGCTAATGTGAGACTACTATTTTCAATAATCCTTAACAATCCTTCCCTTCGAACAAAGTACACCACGAACCCTCCCTTGAGGCTTCCTTTGGAGCTCTCGAACAACCTCCCTTTAATTGAGCCTCGATTCCTTCTTTGGAATACACCCTTTGTTCGACACTTGAGTCACCTTTGACTACACTTTCGAACCTCACCTCCCCTTCGAACAAAGTACACCACAAACCCTCTCTTGGTCTCCTTCTTTGGAGCTCTCAAACAACCTCCCTTATTTGAGGCTCGACTCCTTCTTTGGAATCCTTAAACAAAGTACGCCCTTTGTTCGACATCTGAGCACCTTTGACTACACTTTCGAGCCTTACAACTTCTTTGTTCGATACTTGAGGATTTTATTGGCATGACTAAGTTAAATATATGCCTCTAATACCATGTTAGAAATCACGACCTTCACAATTGTATGATATTGTCCACTTTGAGCATAAATTCTCATGACTTTGCTTTTGGTTTCTCAAAGGTCTTATAACAATACAGATATAATTTTTTAGTTATAAATCCACGACGGACTACTCCTCTCAATAATATTCAACAGATTCTGTGTACTCATACCTGAAAACCTTTATTTATGTCTAAATGCTATCTAAATTTTTAACGAACCTATTAGATGATTTTGTAAGTTATGTAACTAAAATAAACCCAAAATGTTTGCTGATGTGACCCAATCATTTAATATTTCAGGTGTAGACATGGGTATTTCCATGTAGTAAACAATGACTACACTCACTGGGTAATGTATGCCATTGGTGGAAGTGCTAATCCCACCATTAATAGCCAGGGCAACCGATATCTTGCCCCCGTCAACCCTTTTGCCAAAGAGGTATCTTTGTAGAACATTTCAAATATAGCTCAATAAATAAGATATCAATTAACATTGTAACGATCGATTAACCCGTTCTGATTGTTAATATGTGAAGGTCACAAAGAGAGTTGTCACATCTAACGGTGTATGGAGGCATTGGAATTGGAGATCGGAGGGAGATCTCATGCTAAATGGAGCCTATTTCACTCCATCAGGGGCTGGTGCAGGAGCCAGTTATGCAAGAGCCTCAAGCTTAGGAGCCAAATCCTCTTCCTTGGTGGGCTCGCTTACTTCAAATGCA

mRNA sequence

CATTCAATTCCCTCTCTTTCGCTTCACAACTACTCGCATTTAACGCTCTCATACAAATCCCACAGATCGAGCGATCGAGAGATCGAGAGATACAATACACAGTCGCTTAAAATTTTTTCGGAGAAGCAGAGAAAGCTTCAGAATCATCAATAATGGCGGTCACTCTTAGACTACAGTTTTCAACCGCTTCATTAATGCTCTTCCTTCTTCTCAACATCCTCTTCGTTCAATCCAGTTTTGCTGGAGATCAGCGATTGCAGAGCTTTAATTCTTCATCCATGGCGGACCGTTTTTCTGGAGATGAGCGACTGCAGAGCTTCAATTCTTCATCCATGGCGGACCGCTTTGCTGGAGATCAGCGATTGCAGAGCTTCAATTCTTCATCCATGGCGGACCGGTATGTTAAGAATGAGCATGCCGTTGATAATCCAGAGGAAATTGCATCAATGGTGGATTTGAGCATACGCAACAGCACAGAGAGAAGGAACTTGGGGTTCTTCTCTTGTGGAACAGGAAATCCAATTGATGATTGTTGGCGTTGTGACCCACGTTGGCAACTGCGGCGAAAGCGCCTCGCCAATTGCGGCATTGGGTTTGGTCGTAATGCAGTCGGTGGCCGTGATGGGCGATACTACGTCGTTTCTGATCCACGTGACGACGATCCTGTCAATCCCAGACCAGGCACCCTCCGCCATGCTGTGATTCAAGACAGACCATTATGGATTGTGTTCAAACGAGACATGGTGATCACTTTAAAGCAAGAACTCATCATGAACAGCTTCAAGACAATTGATGGGCGTGGCGTCAATGTCCACATCGCTTATGGTGCTTGTATTACAATCCAATTCATTACCAATGTTATTATTCATGGTTTGCATATTCATGACTGCAAGCCAACGGGGAATGCCATGGTTCGAAGCTCGCCTAGTCATTATGGATGGAGGACAATGGCTGATGGTGATGGGATTTCAATTTTTGGGTCGAGTCATATTTGGATTGACCATAATTCTCTTTCTTCTTGTTCTGATGGGCTCATTGATGCTATCATGGGATCAACTGCTATCACAATTTCTAACAATTACTTCACCCACCACAATGAGGTGATGCTCTTGGGACATAGTGACTCTTATGTAAGAGACAAGCAAATGCAAGTCACCATTGCTTATAACCATTTTGGTGAAGGTCTCATCCAAAGAATGCCAAGGTGTAGACATGGGTATTTCCATGTAGTAAACAATGACTACACTCACTGGGTAATGTATGCCATTGGTGGAAGTGCTAATCCCACCATTAATAGCCAGGGCAACCGATATCTTGCCCCCGTCAACCCTTTTGCCAAAGAGGTCACAAAGAGAGTTGTCACATCTAACGGTGTATGGAGGCATTGGAATTGGAGATCGGAGGGAGATCTCATGCTAAATGGAGCCTATTTCACTCCATCAGGGGCTGGTGCAGGAGCCAGTTATGCAAGAGCCTCAAGCTTAGGAGCCAAATCCTCTTCCTTGGTGGGCTCGCTTACTTCAAATGCA

Coding sequence (CDS)

ATGGCGGTCACTCTTAGACTACAGTTTTCAACCGCTTCATTAATGCTCTTCCTTCTTCTCAACATCCTCTTCGTTCAATCCAGTTTTGCTGGAGATCAGCGATTGCAGAGCTTTAATTCTTCATCCATGGCGGACCGTTTTTCTGGAGATGAGCGACTGCAGAGCTTCAATTCTTCATCCATGGCGGACCGCTTTGCTGGAGATCAGCGATTGCAGAGCTTCAATTCTTCATCCATGGCGGACCGGTATGTTAAGAATGAGCATGCCGTTGATAATCCAGAGGAAATTGCATCAATGGTGGATTTGAGCATACGCAACAGCACAGAGAGAAGGAACTTGGGGTTCTTCTCTTGTGGAACAGGAAATCCAATTGATGATTGTTGGCGTTGTGACCCACGTTGGCAACTGCGGCGAAAGCGCCTCGCCAATTGCGGCATTGGGTTTGGTCGTAATGCAGTCGGTGGCCGTGATGGGCGATACTACGTCGTTTCTGATCCACGTGACGACGATCCTGTCAATCCCAGACCAGGCACCCTCCGCCATGCTGTGATTCAAGACAGACCATTATGGATTGTGTTCAAACGAGACATGGTGATCACTTTAAAGCAAGAACTCATCATGAACAGCTTCAAGACAATTGATGGGCGTGGCGTCAATGTCCACATCGCTTATGGTGCTTGTATTACAATCCAATTCATTACCAATGTTATTATTCATGGTTTGCATATTCATGACTGCAAGCCAACGGGGAATGCCATGGTTCGAAGCTCGCCTAGTCATTATGGATGGAGGACAATGGCTGATGGTGATGGGATTTCAATTTTTGGGTCGAGTCATATTTGGATTGACCATAATTCTCTTTCTTCTTGTTCTGATGGGCTCATTGATGCTATCATGGGATCAACTGCTATCACAATTTCTAACAATTACTTCACCCACCACAATGAGGTGATGCTCTTGGGACATAGTGACTCTTATGTAAGAGACAAGCAAATGCAAGTCACCATTGCTTATAACCATTTTGGTGAAGGTCTCATCCAAAGAATGCCAAGGTGTAGACATGGGTATTTCCATGTAGTAAACAATGACTACACTCACTGGGTAATGTATGCCATTGGTGGAAGTGCTAATCCCACCATTAATAGCCAGGGCAACCGATATCTTGCCCCCGTCAACCCTTTTGCCAAAGAGGTCACAAAGAGAGTTGTCACATCTAACGGTGTATGGAGGCATTGGAATTGGAGATCGGAGGGAGATCTCATGCTAAATGGAGCCTATTTCACTCCATCAGGGGCTGGTGCAGGAGCCAGTTATGCAAGAGCCTCAAGCTTAGGAGCCAAATCCTCTTCCTTGGTGGGCTCGCTTACTTCAAATGCA

Protein sequence

MAVTLRLQFSTASLMLFLLLNILFVQSSFAGDQRLQSFNSSSMADRFSGDERLQSFNSSSMADRFAGDQRLQSFNSSSMADRYVKNEHAVDNPEEIASMVDLSIRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKRLANCGIGFGRNAVGGRDGRYYVVSDPRDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDGRGVNVHIAYGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWIDHNSLSSCSDGLIDAIMGSTAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSANPTINSQGNRYLAPVNPFAKEVTKRVVTSNGVWRHWNWRSEGDLMLNGAYFTPSGAGAGASYARASSLGAKSSSLVGSLTSNA
Homology
BLAST of Carg05405 vs. NCBI nr
Match: KAG7011311.1 (putative pectate lyase 8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 928.3 bits (2398), Expect = 2.5e-266
Identity = 459/459 (100.00%), Postives = 459/459 (100.00%), Query Frame = 0

Query: 1   MAVTLRLQFSTASLMLFLLLNILFVQSSFAGDQRLQSFNSSSMADRFSGDERLQSFNSSS 60
           MAVTLRLQFSTASLMLFLLLNILFVQSSFAGDQRLQSFNSSSMADRFSGDERLQSFNSSS
Sbjct: 1   MAVTLRLQFSTASLMLFLLLNILFVQSSFAGDQRLQSFNSSSMADRFSGDERLQSFNSSS 60

Query: 61  MADRFAGDQRLQSFNSSSMADRYVKNEHAVDNPEEIASMVDLSIRNSTERRNLGFFSCGT 120
           MADRFAGDQRLQSFNSSSMADRYVKNEHAVDNPEEIASMVDLSIRNSTERRNLGFFSCGT
Sbjct: 61  MADRFAGDQRLQSFNSSSMADRYVKNEHAVDNPEEIASMVDLSIRNSTERRNLGFFSCGT 120

Query: 121 GNPIDDCWRCDPRWQLRRKRLANCGIGFGRNAVGGRDGRYYVVSDPRDDDPVNPRPGTLR 180
           GNPIDDCWRCDPRWQLRRKRLANCGIGFGRNAVGGRDGRYYVVSDPRDDDPVNPRPGTLR
Sbjct: 121 GNPIDDCWRCDPRWQLRRKRLANCGIGFGRNAVGGRDGRYYVVSDPRDDDPVNPRPGTLR 180

Query: 181 HAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDGRGVNVHIAYGACITIQFITNVIIHG 240
           HAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDGRGVNVHIAYGACITIQFITNVIIHG
Sbjct: 181 HAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDGRGVNVHIAYGACITIQFITNVIIHG 240

Query: 241 LHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWIDHNSLSSCSDGLIDAIMG 300
           LHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWIDHNSLSSCSDGLIDAIMG
Sbjct: 241 LHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWIDHNSLSSCSDGLIDAIMG 300

Query: 301 STAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVV 360
           STAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVV
Sbjct: 301 STAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVV 360

Query: 361 NNDYTHWVMYAIGGSANPTINSQGNRYLAPVNPFAKEVTKRVVTSNGVWRHWNWRSEGDL 420
           NNDYTHWVMYAIGGSANPTINSQGNRYLAPVNPFAKEVTKRVVTSNGVWRHWNWRSEGDL
Sbjct: 361 NNDYTHWVMYAIGGSANPTINSQGNRYLAPVNPFAKEVTKRVVTSNGVWRHWNWRSEGDL 420

Query: 421 MLNGAYFTPSGAGAGASYARASSLGAKSSSLVGSLTSNA 460
           MLNGAYFTPSGAGAGASYARASSLGAKSSSLVGSLTSNA
Sbjct: 421 MLNGAYFTPSGAGAGASYARASSLGAKSSSLVGSLTSNA 459

BLAST of Carg05405 vs. NCBI nr
Match: XP_022963623.1 (probable pectate lyase 8 isoform X2 [Cucurbita moschata])

HSP 1 Score: 928.3 bits (2398), Expect = 2.5e-266
Identity = 459/459 (100.00%), Postives = 459/459 (100.00%), Query Frame = 0

Query: 1   MAVTLRLQFSTASLMLFLLLNILFVQSSFAGDQRLQSFNSSSMADRFSGDERLQSFNSSS 60
           MAVTLRLQFSTASLMLFLLLNILFVQSSFAGDQRLQSFNSSSMADRFSGDERLQSFNSSS
Sbjct: 1   MAVTLRLQFSTASLMLFLLLNILFVQSSFAGDQRLQSFNSSSMADRFSGDERLQSFNSSS 60

Query: 61  MADRFAGDQRLQSFNSSSMADRYVKNEHAVDNPEEIASMVDLSIRNSTERRNLGFFSCGT 120
           MADRFAGDQRLQSFNSSSMADRYVKNEHAVDNPEEIASMVDLSIRNSTERRNLGFFSCGT
Sbjct: 61  MADRFAGDQRLQSFNSSSMADRYVKNEHAVDNPEEIASMVDLSIRNSTERRNLGFFSCGT 120

Query: 121 GNPIDDCWRCDPRWQLRRKRLANCGIGFGRNAVGGRDGRYYVVSDPRDDDPVNPRPGTLR 180
           GNPIDDCWRCDPRWQLRRKRLANCGIGFGRNAVGGRDGRYYVVSDPRDDDPVNPRPGTLR
Sbjct: 121 GNPIDDCWRCDPRWQLRRKRLANCGIGFGRNAVGGRDGRYYVVSDPRDDDPVNPRPGTLR 180

Query: 181 HAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDGRGVNVHIAYGACITIQFITNVIIHG 240
           HAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDGRGVNVHIAYGACITIQFITNVIIHG
Sbjct: 181 HAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDGRGVNVHIAYGACITIQFITNVIIHG 240

Query: 241 LHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWIDHNSLSSCSDGLIDAIMG 300
           LHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWIDHNSLSSCSDGLIDAIMG
Sbjct: 241 LHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWIDHNSLSSCSDGLIDAIMG 300

Query: 301 STAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVV 360
           STAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVV
Sbjct: 301 STAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVV 360

Query: 361 NNDYTHWVMYAIGGSANPTINSQGNRYLAPVNPFAKEVTKRVVTSNGVWRHWNWRSEGDL 420
           NNDYTHWVMYAIGGSANPTINSQGNRYLAPVNPFAKEVTKRVVTSNGVWRHWNWRSEGDL
Sbjct: 361 NNDYTHWVMYAIGGSANPTINSQGNRYLAPVNPFAKEVTKRVVTSNGVWRHWNWRSEGDL 420

Query: 421 MLNGAYFTPSGAGAGASYARASSLGAKSSSLVGSLTSNA 460
           MLNGAYFTPSGAGAGASYARASSLGAKSSSLVGSLTSNA
Sbjct: 421 MLNGAYFTPSGAGAGASYARASSLGAKSSSLVGSLTSNA 459

BLAST of Carg05405 vs. NCBI nr
Match: XP_022963622.1 (probable pectate lyase 8 isoform X1 [Cucurbita moschata])

HSP 1 Score: 922.9 bits (2384), Expect = 1.1e-264
Identity = 459/462 (99.35%), Postives = 459/462 (99.35%), Query Frame = 0

Query: 1   MAVTLRLQFSTASLMLFLLLNILFVQSSFAGDQRLQSFNSSSMADR---FSGDERLQSFN 60
           MAVTLRLQFSTASLMLFLLLNILFVQSSFAGDQRLQSFNSSSMADR   FSGDERLQSFN
Sbjct: 1   MAVTLRLQFSTASLMLFLLLNILFVQSSFAGDQRLQSFNSSSMADRVDSFSGDERLQSFN 60

Query: 61  SSSMADRFAGDQRLQSFNSSSMADRYVKNEHAVDNPEEIASMVDLSIRNSTERRNLGFFS 120
           SSSMADRFAGDQRLQSFNSSSMADRYVKNEHAVDNPEEIASMVDLSIRNSTERRNLGFFS
Sbjct: 61  SSSMADRFAGDQRLQSFNSSSMADRYVKNEHAVDNPEEIASMVDLSIRNSTERRNLGFFS 120

Query: 121 CGTGNPIDDCWRCDPRWQLRRKRLANCGIGFGRNAVGGRDGRYYVVSDPRDDDPVNPRPG 180
           CGTGNPIDDCWRCDPRWQLRRKRLANCGIGFGRNAVGGRDGRYYVVSDPRDDDPVNPRPG
Sbjct: 121 CGTGNPIDDCWRCDPRWQLRRKRLANCGIGFGRNAVGGRDGRYYVVSDPRDDDPVNPRPG 180

Query: 181 TLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDGRGVNVHIAYGACITIQFITNVI 240
           TLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDGRGVNVHIAYGACITIQFITNVI
Sbjct: 181 TLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDGRGVNVHIAYGACITIQFITNVI 240

Query: 241 IHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWIDHNSLSSCSDGLIDA 300
           IHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWIDHNSLSSCSDGLIDA
Sbjct: 241 IHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWIDHNSLSSCSDGLIDA 300

Query: 301 IMGSTAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYF 360
           IMGSTAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYF
Sbjct: 301 IMGSTAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYF 360

Query: 361 HVVNNDYTHWVMYAIGGSANPTINSQGNRYLAPVNPFAKEVTKRVVTSNGVWRHWNWRSE 420
           HVVNNDYTHWVMYAIGGSANPTINSQGNRYLAPVNPFAKEVTKRVVTSNGVWRHWNWRSE
Sbjct: 361 HVVNNDYTHWVMYAIGGSANPTINSQGNRYLAPVNPFAKEVTKRVVTSNGVWRHWNWRSE 420

Query: 421 GDLMLNGAYFTPSGAGAGASYARASSLGAKSSSLVGSLTSNA 460
           GDLMLNGAYFTPSGAGAGASYARASSLGAKSSSLVGSLTSNA
Sbjct: 421 GDLMLNGAYFTPSGAGAGASYARASSLGAKSSSLVGSLTSNA 462

BLAST of Carg05405 vs. NCBI nr
Match: XP_023553696.1 (probable pectate lyase 8 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 916.8 bits (2368), Expect = 7.6e-263
Identity = 452/459 (98.47%), Postives = 455/459 (99.13%), Query Frame = 0

Query: 1   MAVTLRLQFSTASLMLFLLLNILFVQSSFAGDQRLQSFNSSSMADRFSGDERLQSFNSSS 60
           MAVTLRLQFSTASLM FLLLNILFVQSSFAGDQRLQSFNSSSMAD F+GD+RLQSFNSSS
Sbjct: 1   MAVTLRLQFSTASLMFFLLLNILFVQSSFAGDQRLQSFNSSSMADSFAGDQRLQSFNSSS 60

Query: 61  MADRFAGDQRLQSFNSSSMADRYVKNEHAVDNPEEIASMVDLSIRNSTERRNLGFFSCGT 120
           MAD F+GDQRLQSFNSSSMADRYVKNEHAVDNPEEIASMVDLSIRNSTERRNLGFFSCGT
Sbjct: 61  MADSFSGDQRLQSFNSSSMADRYVKNEHAVDNPEEIASMVDLSIRNSTERRNLGFFSCGT 120

Query: 121 GNPIDDCWRCDPRWQLRRKRLANCGIGFGRNAVGGRDGRYYVVSDPRDDDPVNPRPGTLR 180
           GNPIDDCWRCDP WQLRRKRLANCGIGFGRNAVGGRDGRYYVVSDPRDDDPVNPRPGTLR
Sbjct: 121 GNPIDDCWRCDPHWQLRRKRLANCGIGFGRNAVGGRDGRYYVVSDPRDDDPVNPRPGTLR 180

Query: 181 HAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDGRGVNVHIAYGACITIQFITNVIIHG 240
           HAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDGRGVNVHIAYGACITIQFITNVIIHG
Sbjct: 181 HAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDGRGVNVHIAYGACITIQFITNVIIHG 240

Query: 241 LHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWIDHNSLSSCSDGLIDAIMG 300
           LHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWIDHNSLSSCSDGLIDAIMG
Sbjct: 241 LHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWIDHNSLSSCSDGLIDAIMG 300

Query: 301 STAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVV 360
           STAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVV
Sbjct: 301 STAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVV 360

Query: 361 NNDYTHWVMYAIGGSANPTINSQGNRYLAPVNPFAKEVTKRVVTSNGVWRHWNWRSEGDL 420
           NNDYTHWVMYAIGGSANPTINSQGNRYLAPVNPFAKEVTKRVVTSNGVWRHWNWRSEGDL
Sbjct: 361 NNDYTHWVMYAIGGSANPTINSQGNRYLAPVNPFAKEVTKRVVTSNGVWRHWNWRSEGDL 420

Query: 421 MLNGAYFTPSGAGAGASYARASSLGAKSSSLVGSLTSNA 460
           MLNGAYFTPSGAGAGASYARASSLGAKSSSLVGSLTSNA
Sbjct: 421 MLNGAYFTPSGAGAGASYARASSLGAKSSSLVGSLTSNA 459

BLAST of Carg05405 vs. NCBI nr
Match: KAG6571569.1 (putative pectate lyase 15, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 906.4 bits (2341), Expect = 1.0e-259
Identity = 445/445 (100.00%), Postives = 445/445 (100.00%), Query Frame = 0

Query: 15  MLFLLLNILFVQSSFAGDQRLQSFNSSSMADRFSGDERLQSFNSSSMADRFAGDQRLQSF 74
           MLFLLLNILFVQSSFAGDQRLQSFNSSSMADRFSGDERLQSFNSSSMADRFAGDQRLQSF
Sbjct: 1   MLFLLLNILFVQSSFAGDQRLQSFNSSSMADRFSGDERLQSFNSSSMADRFAGDQRLQSF 60

Query: 75  NSSSMADRYVKNEHAVDNPEEIASMVDLSIRNSTERRNLGFFSCGTGNPIDDCWRCDPRW 134
           NSSSMADRYVKNEHAVDNPEEIASMVDLSIRNSTERRNLGFFSCGTGNPIDDCWRCDPRW
Sbjct: 61  NSSSMADRYVKNEHAVDNPEEIASMVDLSIRNSTERRNLGFFSCGTGNPIDDCWRCDPRW 120

Query: 135 QLRRKRLANCGIGFGRNAVGGRDGRYYVVSDPRDDDPVNPRPGTLRHAVIQDRPLWIVFK 194
           QLRRKRLANCGIGFGRNAVGGRDGRYYVVSDPRDDDPVNPRPGTLRHAVIQDRPLWIVFK
Sbjct: 121 QLRRKRLANCGIGFGRNAVGGRDGRYYVVSDPRDDDPVNPRPGTLRHAVIQDRPLWIVFK 180

Query: 195 RDMVITLKQELIMNSFKTIDGRGVNVHIAYGACITIQFITNVIIHGLHIHDCKPTGNAMV 254
           RDMVITLKQELIMNSFKTIDGRGVNVHIAYGACITIQFITNVIIHGLHIHDCKPTGNAMV
Sbjct: 181 RDMVITLKQELIMNSFKTIDGRGVNVHIAYGACITIQFITNVIIHGLHIHDCKPTGNAMV 240

Query: 255 RSSPSHYGWRTMADGDGISIFGSSHIWIDHNSLSSCSDGLIDAIMGSTAITISNNYFTHH 314
           RSSPSHYGWRTMADGDGISIFGSSHIWIDHNSLSSCSDGLIDAIMGSTAITISNNYFTHH
Sbjct: 241 RSSPSHYGWRTMADGDGISIFGSSHIWIDHNSLSSCSDGLIDAIMGSTAITISNNYFTHH 300

Query: 315 NEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGG 374
           NEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGG
Sbjct: 301 NEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGG 360

Query: 375 SANPTINSQGNRYLAPVNPFAKEVTKRVVTSNGVWRHWNWRSEGDLMLNGAYFTPSGAGA 434
           SANPTINSQGNRYLAPVNPFAKEVTKRVVTSNGVWRHWNWRSEGDLMLNGAYFTPSGAGA
Sbjct: 361 SANPTINSQGNRYLAPVNPFAKEVTKRVVTSNGVWRHWNWRSEGDLMLNGAYFTPSGAGA 420

Query: 435 GASYARASSLGAKSSSLVGSLTSNA 460
           GASYARASSLGAKSSSLVGSLTSNA
Sbjct: 421 GASYARASSLGAKSSSLVGSLTSNA 445

BLAST of Carg05405 vs. ExPASy Swiss-Prot
Match: Q93WF1 (Probable pectate lyase 20 OS=Arabidopsis thaliana OX=3702 GN=At5g48900 PE=2 SV=1)

HSP 1 Score: 688.3 bits (1775), Expect = 5.8e-197
Identity = 322/374 (86.10%), Postives = 345/374 (92.25%), Query Frame = 0

Query: 86  NEHAVDNPEEIASMVDLSIRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKRLANCG 145
           +EHAV+NPEE+A+MVD+SIRNST RR LG+FSC TGNPIDDCWRCD RWQ RRK LANC 
Sbjct: 33  HEHAVENPEEVAAMVDMSIRNSTARRRLGYFSCSTGNPIDDCWRCDRRWQSRRKHLANCA 92

Query: 146 IGFGRNAVGGRDGRYYVVSDPRDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQEL 205
           IGFGRNA+GGRDGRYYVVSDP DD+PVNP+PGTLRHAVIQ+ PLWIVFKRDMVITLK+EL
Sbjct: 93  IGFGRNAIGGRDGRYYVVSDPNDDNPVNPKPGTLRHAVIQEEPLWIVFKRDMVITLKEEL 152

Query: 206 IMNSFKTIDGRGVNVHIAYGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRT 265
           IMNSFKTIDGRGVNVHIA GACITIQF+TN+IIHG+HIHDC+PTGNAMVRSSPSHYGWRT
Sbjct: 153 IMNSFKTIDGRGVNVHIANGACITIQFVTNIIIHGIHIHDCRPTGNAMVRSSPSHYGWRT 212

Query: 266 MADGDGISIFGSSHIWIDHNSLSSCSDGLIDAIMGSTAITISNNYFTHHNEVMLLGHSDS 325
           MADGDGISIFGSSHIWIDHNSLS+C+DGLIDA+M STAITISNNYFTHHNEVMLLGHSD+
Sbjct: 213 MADGDGISIFGSSHIWIDHNSLSNCADGLIDAVMASTAITISNNYFTHHNEVMLLGHSDT 272

Query: 326 YVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSANPTINSQGN 385
           Y RDK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHW MYAIGGSA+PTINSQGN
Sbjct: 273 YTRDKVMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTINSQGN 332

Query: 386 RYLAPVNPFAKEVTKRVVTSNGVWRHWNWRSEGDLMLNGAYFTPSGAGAGASYARASSLG 445
           RYLAP N FAKEVTKR       WRHWNWRSEGDL LNGA+FT SG+G GASYARASSL 
Sbjct: 333 RYLAPRNRFAKEVTKRDYAGQWQWRHWNWRSEGDLFLNGAFFTRSGSGLGASYARASSLA 392

Query: 446 AKSSSLVGSLTSNA 460
           AKSSSLVG +T NA
Sbjct: 393 AKSSSLVGVITYNA 406

BLAST of Carg05405 vs. ExPASy Swiss-Prot
Match: Q9M8Z8 (Probable pectate lyase 8 OS=Arabidopsis thaliana OX=3702 GN=At3g07010 PE=2 SV=1)

HSP 1 Score: 687.2 bits (1772), Expect = 1.3e-196
Identity = 315/372 (84.68%), Postives = 349/372 (93.82%), Query Frame = 0

Query: 86  NEHAVDNPEEIASMVDLSIRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKRLANCG 145
           +EHAV+NP+E+A+MVD+SIRNSTERR LG+FSC TGNPIDDCWRCD +WQLRRKRLA+C 
Sbjct: 32  HEHAVENPDEVAAMVDMSIRNSTERRRLGYFSCATGNPIDDCWRCDRKWQLRRKRLADCS 91

Query: 146 IGFGRNAVGGRDGRYYVVSDPRDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQEL 205
           IGFGRNA+GGRDGR+YVV+DP DDDPVNP PGTLRHAVIQD PLWI+FKRDMVITLKQEL
Sbjct: 92  IGFGRNAIGGRDGRFYVVTDPGDDDPVNPIPGTLRHAVIQDEPLWIIFKRDMVITLKQEL 151

Query: 206 IMNSFKTIDGRGVNVHIAYGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRT 265
           IMNSFKTIDGRGVNVHIA GAC+TIQ++TN+I+HG+H+HDCKPTGNAMVRSSPSHYG+R+
Sbjct: 152 IMNSFKTIDGRGVNVHIANGACLTIQYVTNIIVHGIHVHDCKPTGNAMVRSSPSHYGFRS 211

Query: 266 MADGDGISIFGSSHIWIDHNSLSSCSDGLIDAIMGSTAITISNNYFTHHNEVMLLGHSDS 325
           MADGD ISIFGSSHIWIDHNSLS+C+DGL+DA+M STAIT+SNN+FTHHNEVMLLGHSDS
Sbjct: 212 MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMSSTAITVSNNFFTHHNEVMLLGHSDS 271

Query: 326 YVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSANPTINSQGN 385
           Y RDK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHW MYAIGGSA PTINSQGN
Sbjct: 272 YTRDKVMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAGPTINSQGN 331

Query: 386 RYLAPVNPFAKEVTKRVVTSNGVWRHWNWRSEGDLMLNGAYFTPSGAGAGASYARASSLG 445
           R+LAPVNPFAKEVTKR  T    W+HWNWRSEGDL LNGA+FT SGAGAGA+YARASSL 
Sbjct: 332 RFLAPVNPFAKEVTKREYTGESKWKHWNWRSEGDLFLNGAFFTRSGAGAGANYARASSLS 391

Query: 446 AKSSSLVGSLTS 458
           AKSSSLVG++TS
Sbjct: 392 AKSSSLVGTMTS 403

BLAST of Carg05405 vs. ExPASy Swiss-Prot
Match: Q9LJ42 (Probable pectate lyase 10 OS=Arabidopsis thaliana OX=3702 GN=At3g24670 PE=2 SV=2)

HSP 1 Score: 676.8 bits (1745), Expect = 1.7e-193
Identity = 328/405 (80.99%), Postives = 355/405 (87.65%), Query Frame = 0

Query: 57  NSSSMADRFAGDQRLQSFNSSSMADRYVK-----NEHAVDNPEEIASMVDLSIRNSTERR 116
           NSS+MA     D    S ++SS A++        NEHAV NPEE+A+MVD+ I+NSTERR
Sbjct: 23  NSSTMAQE-TEDLNSHSSSNSSTANKLPNDDGAWNEHAVKNPEEVAAMVDMKIKNSTERR 82

Query: 117 NLGFFSCGTGNPIDDCWRCDPRWQLRRKRLANCGIGFGRNAVGGRDGRYYVVSDPRDDDP 176
            LGFFSC TGNPIDDCWRCD  W LRRKRLANC IGFGRNA+GGRDGRYYVV+DP D D 
Sbjct: 83  RLGFFSCATGNPIDDCWRCDRNWHLRRKRLANCAIGFGRNAIGGRDGRYYVVTDPSDHDA 142

Query: 177 VNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDGRGVNVHIAYGACITIQ 236
           VNPRPGTLRHAVIQDRPLWIVFKRDMVITL QELIMNSFKTIDGRGVNV IA GACITIQ
Sbjct: 143 VNPRPGTLRHAVIQDRPLWIVFKRDMVITLTQELIMNSFKTIDGRGVNVAIAGGACITIQ 202

Query: 237 FITNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWIDHNSLSSCS 296
           ++TN+IIHG+++HDC+ TGNAMVRSSPSHYGWRTMADGD ISIFGSSHIWIDHNSLS+C+
Sbjct: 203 YVTNIIIHGINVHDCRRTGNAMVRSSPSHYGWRTMADGDAISIFGSSHIWIDHNSLSNCA 262

Query: 297 DGLIDAIMGSTAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPR 356
           DGLIDAIMGSTAITISNNY THHNEVML+GHSDSY RDK MQVTIAYNHFGEGLIQRMPR
Sbjct: 263 DGLIDAIMGSTAITISNNYMTHHNEVMLMGHSDSYTRDKLMQVTIAYNHFGEGLIQRMPR 322

Query: 357 CRHGYFHVVNNDYTHWVMYAIGGSANPTINSQGNRYLAPVNPFAKEVTKRVVTSNGVWRH 416
           CRHGYFHVVNNDYTHWVMYAIGGSANPTINSQGNR+LAP NPFAKEVTKRV +  G W+ 
Sbjct: 323 CRHGYFHVVNNDYTHWVMYAIGGSANPTINSQGNRFLAPGNPFAKEVTKRVGSWQGEWKQ 382

Query: 417 WNWRSEGDLMLNGAYFTPSGAGAGASYARASSLGAKSSSLVGSLT 457
           WNWRS+GDLMLNGAYFT SGA A ASYARASSLGAK +S+V  LT
Sbjct: 383 WNWRSQGDLMLNGAYFTKSGAAAPASYARASSLGAKPASVVSMLT 426

BLAST of Carg05405 vs. ExPASy Swiss-Prot
Match: Q940Q1 (Probable pectate lyase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g04680 PE=2 SV=2)

HSP 1 Score: 665.2 bits (1715), Expect = 5.2e-190
Identity = 312/391 (79.80%), Postives = 345/391 (88.24%), Query Frame = 0

Query: 73  SFNSSSMADRYVKNEHAVDNPEEIA----SMVDLSIRNSTERRNLGFFSCGTGNPIDDCW 132
           + +S   +D    N+HAV NP+E+A    ++ ++S+RN TERR LG+F+CGTGNPIDDCW
Sbjct: 28  NISSLPRSDETEWNQHAVTNPDEVADEVLALTEMSVRNHTERRKLGYFTCGTGNPIDDCW 87

Query: 133 RCDPRWQLRRKRLANCGIGFGRNAVGGRDGRYYVVSDPRDDDPVNPRPGTLRHAVIQDRP 192
           RCDP W   RKRLA+CGIGFGRNA+GGRDGR+YVV+DPRDD+PVNPRPGTLRHAVIQDRP
Sbjct: 88  RCDPNWHKNRKRLADCGIGFGRNAIGGRDGRFYVVTDPRDDNPVNPRPGTLRHAVIQDRP 147

Query: 193 LWIVFKRDMVITLKQELIMNSFKTIDGRGVNVHIAYGACITIQFITNVIIHGLHIHDCKP 252
           LWIVFKRDMVI LKQELI+NSFKTIDGRG NVHIA G CITIQF+TNVI+HGLHIHDCKP
Sbjct: 148 LWIVFKRDMVIQLKQELIVNSFKTIDGRGANVHIANGGCITIQFVTNVIVHGLHIHDCKP 207

Query: 253 TGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWIDHNSLSSCSDGLIDAIMGSTAITISN 312
           TGNAMVRSS +H+GWRTMADGD ISIFGSSH+WIDHNSLS C+DGL+DA+MGSTAITISN
Sbjct: 208 TGNAMVRSSETHFGWRTMADGDAISIFGSSHVWIDHNSLSHCADGLVDAVMGSTAITISN 267

Query: 313 NYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWV 372
           N+ THHNEVMLLGHSDSY+RDK MQVTIAYNHFG GLIQRMPRCRHGYFHVVNNDYTHW 
Sbjct: 268 NHLTHHNEVMLLGHSDSYMRDKAMQVTIAYNHFGVGLIQRMPRCRHGYFHVVNNDYTHWE 327

Query: 373 MYAIGGSANPTINSQGNRYLAPVNPFAKEVTKRVVTSNGVWRHWNWRSEGDLMLNGAYFT 432
           MYAIGGSANPTINSQGNRY AP NPFAKEVTKRV T    W+ WNWRSEGDL+ NGAYFT
Sbjct: 328 MYAIGGSANPTINSQGNRYAAPKNPFAKEVTKRVDTPASHWKGWNWRSEGDLLQNGAYFT 387

Query: 433 PSGAGAGASYARASSLGAKSSSLVGSLTSNA 460
            SGA A  SYARASSL AKSSSLVG +TS+A
Sbjct: 388 SSGAAASGSYARASSLSAKSSSLVGHITSDA 418

BLAST of Carg05405 vs. ExPASy Swiss-Prot
Match: Q9SVQ6 (Putative pectate lyase 14 OS=Arabidopsis thaliana OX=3702 GN=At4g13210 PE=3 SV=2)

HSP 1 Score: 664.8 bits (1714), Expect = 6.8e-190
Identity = 315/375 (84.00%), Postives = 341/375 (90.93%), Query Frame = 0

Query: 86  NEHAVDNPEEIASMVDLSIRNSTERRNLGFF-SCGTGNPIDDCWRCDPRWQLRRKRLANC 145
           +EHAV +PEEIA+MVD+SIRNST RR LGFF SC TGNPIDDCWRCD +W  RRKRLA+C
Sbjct: 53  HEHAVKDPEEIAAMVDMSIRNSTYRRKLGFFSSCSTGNPIDDCWRCDKKWHRRRKRLADC 112

Query: 146 GIGFGRNAVGGRDGRYYVVSDPRDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQE 205
            IGFGRNAVGGRDGRYY+V+DP D DPV P+PGTLR+AVIQD PLWIVFKRDMVITL QE
Sbjct: 113 AIGFGRNAVGGRDGRYYIVTDPSDHDPVTPKPGTLRYAVIQDEPLWIVFKRDMVITLSQE 172

Query: 206 LIMNSFKTIDGRGVNVHIAYGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHYGWR 265
           LIMNSFKTIDGRGVNVHIA GAC+T+Q++TN+IIHG++IHDCK TGNAMVRSS SHYGWR
Sbjct: 173 LIMNSFKTIDGRGVNVHIAGGACLTVQYVTNIIIHGINIHDCKRTGNAMVRSSESHYGWR 232

Query: 266 TMADGDGISIFGSSHIWIDHNSLSSCSDGLIDAIMGSTAITISNNYFTHHNEVMLLGHSD 325
           TMADGDGISIFGSSHIWIDHNSLSSC+DGLIDAIMGSTAITISNNY THHNE +LLGH+D
Sbjct: 233 TMADGDGISIFGSSHIWIDHNSLSSCADGLIDAIMGSTAITISNNYLTHHNEAILLGHTD 292

Query: 326 SYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSANPTINSQG 385
           SY RDK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHW MYAIGGSANPTINSQG
Sbjct: 293 SYTRDKMMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQG 352

Query: 386 NRYLAPVNPFAKEVTKRVVTSNGVWRHWNWRSEGDLMLNGAYFTPSGAGAGASYARASSL 445
           NR+LAP N FAKEVTKRV    G W +WNWRS+GDLMLNGAYFT SGAGA A+YARASSL
Sbjct: 353 NRFLAPGNRFAKEVTKRVGAGKGEWNNWNWRSQGDLMLNGAYFTSSGAGASANYARASSL 412

Query: 446 GAKSSSLVGSLTSNA 460
            AKSSSLVG LTS++
Sbjct: 413 AAKSSSLVGMLTSSS 427

BLAST of Carg05405 vs. ExPASy TrEMBL
Match: A0A6J1HIF3 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111463899 PE=3 SV=1)

HSP 1 Score: 928.3 bits (2398), Expect = 1.2e-266
Identity = 459/459 (100.00%), Postives = 459/459 (100.00%), Query Frame = 0

Query: 1   MAVTLRLQFSTASLMLFLLLNILFVQSSFAGDQRLQSFNSSSMADRFSGDERLQSFNSSS 60
           MAVTLRLQFSTASLMLFLLLNILFVQSSFAGDQRLQSFNSSSMADRFSGDERLQSFNSSS
Sbjct: 1   MAVTLRLQFSTASLMLFLLLNILFVQSSFAGDQRLQSFNSSSMADRFSGDERLQSFNSSS 60

Query: 61  MADRFAGDQRLQSFNSSSMADRYVKNEHAVDNPEEIASMVDLSIRNSTERRNLGFFSCGT 120
           MADRFAGDQRLQSFNSSSMADRYVKNEHAVDNPEEIASMVDLSIRNSTERRNLGFFSCGT
Sbjct: 61  MADRFAGDQRLQSFNSSSMADRYVKNEHAVDNPEEIASMVDLSIRNSTERRNLGFFSCGT 120

Query: 121 GNPIDDCWRCDPRWQLRRKRLANCGIGFGRNAVGGRDGRYYVVSDPRDDDPVNPRPGTLR 180
           GNPIDDCWRCDPRWQLRRKRLANCGIGFGRNAVGGRDGRYYVVSDPRDDDPVNPRPGTLR
Sbjct: 121 GNPIDDCWRCDPRWQLRRKRLANCGIGFGRNAVGGRDGRYYVVSDPRDDDPVNPRPGTLR 180

Query: 181 HAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDGRGVNVHIAYGACITIQFITNVIIHG 240
           HAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDGRGVNVHIAYGACITIQFITNVIIHG
Sbjct: 181 HAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDGRGVNVHIAYGACITIQFITNVIIHG 240

Query: 241 LHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWIDHNSLSSCSDGLIDAIMG 300
           LHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWIDHNSLSSCSDGLIDAIMG
Sbjct: 241 LHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWIDHNSLSSCSDGLIDAIMG 300

Query: 301 STAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVV 360
           STAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVV
Sbjct: 301 STAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVV 360

Query: 361 NNDYTHWVMYAIGGSANPTINSQGNRYLAPVNPFAKEVTKRVVTSNGVWRHWNWRSEGDL 420
           NNDYTHWVMYAIGGSANPTINSQGNRYLAPVNPFAKEVTKRVVTSNGVWRHWNWRSEGDL
Sbjct: 361 NNDYTHWVMYAIGGSANPTINSQGNRYLAPVNPFAKEVTKRVVTSNGVWRHWNWRSEGDL 420

Query: 421 MLNGAYFTPSGAGAGASYARASSLGAKSSSLVGSLTSNA 460
           MLNGAYFTPSGAGAGASYARASSLGAKSSSLVGSLTSNA
Sbjct: 421 MLNGAYFTPSGAGAGASYARASSLGAKSSSLVGSLTSNA 459

BLAST of Carg05405 vs. ExPASy TrEMBL
Match: A0A6J1HFR0 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111463899 PE=3 SV=1)

HSP 1 Score: 922.9 bits (2384), Expect = 5.1e-265
Identity = 459/462 (99.35%), Postives = 459/462 (99.35%), Query Frame = 0

Query: 1   MAVTLRLQFSTASLMLFLLLNILFVQSSFAGDQRLQSFNSSSMADR---FSGDERLQSFN 60
           MAVTLRLQFSTASLMLFLLLNILFVQSSFAGDQRLQSFNSSSMADR   FSGDERLQSFN
Sbjct: 1   MAVTLRLQFSTASLMLFLLLNILFVQSSFAGDQRLQSFNSSSMADRVDSFSGDERLQSFN 60

Query: 61  SSSMADRFAGDQRLQSFNSSSMADRYVKNEHAVDNPEEIASMVDLSIRNSTERRNLGFFS 120
           SSSMADRFAGDQRLQSFNSSSMADRYVKNEHAVDNPEEIASMVDLSIRNSTERRNLGFFS
Sbjct: 61  SSSMADRFAGDQRLQSFNSSSMADRYVKNEHAVDNPEEIASMVDLSIRNSTERRNLGFFS 120

Query: 121 CGTGNPIDDCWRCDPRWQLRRKRLANCGIGFGRNAVGGRDGRYYVVSDPRDDDPVNPRPG 180
           CGTGNPIDDCWRCDPRWQLRRKRLANCGIGFGRNAVGGRDGRYYVVSDPRDDDPVNPRPG
Sbjct: 121 CGTGNPIDDCWRCDPRWQLRRKRLANCGIGFGRNAVGGRDGRYYVVSDPRDDDPVNPRPG 180

Query: 181 TLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDGRGVNVHIAYGACITIQFITNVI 240
           TLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDGRGVNVHIAYGACITIQFITNVI
Sbjct: 181 TLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDGRGVNVHIAYGACITIQFITNVI 240

Query: 241 IHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWIDHNSLSSCSDGLIDA 300
           IHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWIDHNSLSSCSDGLIDA
Sbjct: 241 IHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWIDHNSLSSCSDGLIDA 300

Query: 301 IMGSTAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYF 360
           IMGSTAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYF
Sbjct: 301 IMGSTAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYF 360

Query: 361 HVVNNDYTHWVMYAIGGSANPTINSQGNRYLAPVNPFAKEVTKRVVTSNGVWRHWNWRSE 420
           HVVNNDYTHWVMYAIGGSANPTINSQGNRYLAPVNPFAKEVTKRVVTSNGVWRHWNWRSE
Sbjct: 361 HVVNNDYTHWVMYAIGGSANPTINSQGNRYLAPVNPFAKEVTKRVVTSNGVWRHWNWRSE 420

Query: 421 GDLMLNGAYFTPSGAGAGASYARASSLGAKSSSLVGSLTSNA 460
           GDLMLNGAYFTPSGAGAGASYARASSLGAKSSSLVGSLTSNA
Sbjct: 421 GDLMLNGAYFTPSGAGAGASYARASSLGAKSSSLVGSLTSNA 462

BLAST of Carg05405 vs. ExPASy TrEMBL
Match: A0A6J1HKQ9 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111463899 PE=3 SV=1)

HSP 1 Score: 886.3 bits (2289), Expect = 5.3e-254
Identity = 440/459 (95.86%), Postives = 442/459 (96.30%), Query Frame = 0

Query: 1   MAVTLRLQFSTASLMLFLLLNILFVQSSFAGDQRLQSFNSSSMADRFSGDERLQSFNSSS 60
           MAVTLRLQFSTASLMLFLLLNILFVQSSFAGDQRLQSFNSSSMADR              
Sbjct: 1   MAVTLRLQFSTASLMLFLLLNILFVQSSFAGDQRLQSFNSSSMADR-------------- 60

Query: 61  MADRFAGDQRLQSFNSSSMADRYVKNEHAVDNPEEIASMVDLSIRNSTERRNLGFFSCGT 120
             D F+GD+RLQSFNSSSMADRYVKNEHAVDNPEEIASMVDLSIRNSTERRNLGFFSCGT
Sbjct: 61  -VDSFSGDERLQSFNSSSMADRYVKNEHAVDNPEEIASMVDLSIRNSTERRNLGFFSCGT 120

Query: 121 GNPIDDCWRCDPRWQLRRKRLANCGIGFGRNAVGGRDGRYYVVSDPRDDDPVNPRPGTLR 180
           GNPIDDCWRCDPRWQLRRKRLANCGIGFGRNAVGGRDGRYYVVSDPRDDDPVNPRPGTLR
Sbjct: 121 GNPIDDCWRCDPRWQLRRKRLANCGIGFGRNAVGGRDGRYYVVSDPRDDDPVNPRPGTLR 180

Query: 181 HAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDGRGVNVHIAYGACITIQFITNVIIHG 240
           HAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDGRGVNVHIAYGACITIQFITNVIIHG
Sbjct: 181 HAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDGRGVNVHIAYGACITIQFITNVIIHG 240

Query: 241 LHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWIDHNSLSSCSDGLIDAIMG 300
           LHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWIDHNSLSSCSDGLIDAIMG
Sbjct: 241 LHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWIDHNSLSSCSDGLIDAIMG 300

Query: 301 STAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVV 360
           STAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVV
Sbjct: 301 STAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVV 360

Query: 361 NNDYTHWVMYAIGGSANPTINSQGNRYLAPVNPFAKEVTKRVVTSNGVWRHWNWRSEGDL 420
           NNDYTHWVMYAIGGSANPTINSQGNRYLAPVNPFAKEVTKRVVTSNGVWRHWNWRSEGDL
Sbjct: 361 NNDYTHWVMYAIGGSANPTINSQGNRYLAPVNPFAKEVTKRVVTSNGVWRHWNWRSEGDL 420

Query: 421 MLNGAYFTPSGAGAGASYARASSLGAKSSSLVGSLTSNA 460
           MLNGAYFTPSGAGAGASYARASSLGAKSSSLVGSLTSNA
Sbjct: 421 MLNGAYFTPSGAGAGASYARASSLGAKSSSLVGSLTSNA 444

BLAST of Carg05405 vs. ExPASy TrEMBL
Match: A0A6J1HII4 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111463899 PE=3 SV=1)

HSP 1 Score: 885.6 bits (2287), Expect = 9.1e-254
Identity = 441/459 (96.08%), Postives = 441/459 (96.08%), Query Frame = 0

Query: 1   MAVTLRLQFSTASLMLFLLLNILFVQSSFAGDQRLQSFNSSSMADRFSGDERLQSFNSSS 60
           MAVTLRLQFSTASLMLFLLLNILFVQSSFAGDQRLQSFNSSSMADRFSGDE         
Sbjct: 1   MAVTLRLQFSTASLMLFLLLNILFVQSSFAGDQRLQSFNSSSMADRFSGDE--------- 60

Query: 61  MADRFAGDQRLQSFNSSSMADRYVKNEHAVDNPEEIASMVDLSIRNSTERRNLGFFSCGT 120
                    RLQSFNSSSMADRYVKNEHAVDNPEEIASMVDLSIRNSTERRNLGFFSCGT
Sbjct: 61  ---------RLQSFNSSSMADRYVKNEHAVDNPEEIASMVDLSIRNSTERRNLGFFSCGT 120

Query: 121 GNPIDDCWRCDPRWQLRRKRLANCGIGFGRNAVGGRDGRYYVVSDPRDDDPVNPRPGTLR 180
           GNPIDDCWRCDPRWQLRRKRLANCGIGFGRNAVGGRDGRYYVVSDPRDDDPVNPRPGTLR
Sbjct: 121 GNPIDDCWRCDPRWQLRRKRLANCGIGFGRNAVGGRDGRYYVVSDPRDDDPVNPRPGTLR 180

Query: 181 HAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDGRGVNVHIAYGACITIQFITNVIIHG 240
           HAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDGRGVNVHIAYGACITIQFITNVIIHG
Sbjct: 181 HAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDGRGVNVHIAYGACITIQFITNVIIHG 240

Query: 241 LHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWIDHNSLSSCSDGLIDAIMG 300
           LHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWIDHNSLSSCSDGLIDAIMG
Sbjct: 241 LHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWIDHNSLSSCSDGLIDAIMG 300

Query: 301 STAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVV 360
           STAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVV
Sbjct: 301 STAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVV 360

Query: 361 NNDYTHWVMYAIGGSANPTINSQGNRYLAPVNPFAKEVTKRVVTSNGVWRHWNWRSEGDL 420
           NNDYTHWVMYAIGGSANPTINSQGNRYLAPVNPFAKEVTKRVVTSNGVWRHWNWRSEGDL
Sbjct: 361 NNDYTHWVMYAIGGSANPTINSQGNRYLAPVNPFAKEVTKRVVTSNGVWRHWNWRSEGDL 420

Query: 421 MLNGAYFTPSGAGAGASYARASSLGAKSSSLVGSLTSNA 460
           MLNGAYFTPSGAGAGASYARASSLGAKSSSLVGSLTSNA
Sbjct: 421 MLNGAYFTPSGAGAGASYARASSLGAKSSSLVGSLTSNA 441

BLAST of Carg05405 vs. ExPASy TrEMBL
Match: A0A6J1HGK6 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111463899 PE=3 SV=1)

HSP 1 Score: 885.6 bits (2287), Expect = 9.1e-254
Identity = 441/459 (96.08%), Postives = 441/459 (96.08%), Query Frame = 0

Query: 1   MAVTLRLQFSTASLMLFLLLNILFVQSSFAGDQRLQSFNSSSMADRFSGDERLQSFNSSS 60
           MAVTLRLQFSTASLMLFLLLNILFVQSSFAGDQ                  RLQSFNSSS
Sbjct: 1   MAVTLRLQFSTASLMLFLLLNILFVQSSFAGDQ------------------RLQSFNSSS 60

Query: 61  MADRFAGDQRLQSFNSSSMADRYVKNEHAVDNPEEIASMVDLSIRNSTERRNLGFFSCGT 120
           MADRFAGDQRLQSFNSSSMADRYVKNEHAVDNPEEIASMVDLSIRNSTERRNLGFFSCGT
Sbjct: 61  MADRFAGDQRLQSFNSSSMADRYVKNEHAVDNPEEIASMVDLSIRNSTERRNLGFFSCGT 120

Query: 121 GNPIDDCWRCDPRWQLRRKRLANCGIGFGRNAVGGRDGRYYVVSDPRDDDPVNPRPGTLR 180
           GNPIDDCWRCDPRWQLRRKRLANCGIGFGRNAVGGRDGRYYVVSDPRDDDPVNPRPGTLR
Sbjct: 121 GNPIDDCWRCDPRWQLRRKRLANCGIGFGRNAVGGRDGRYYVVSDPRDDDPVNPRPGTLR 180

Query: 181 HAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDGRGVNVHIAYGACITIQFITNVIIHG 240
           HAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDGRGVNVHIAYGACITIQFITNVIIHG
Sbjct: 181 HAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDGRGVNVHIAYGACITIQFITNVIIHG 240

Query: 241 LHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWIDHNSLSSCSDGLIDAIMG 300
           LHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWIDHNSLSSCSDGLIDAIMG
Sbjct: 241 LHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWIDHNSLSSCSDGLIDAIMG 300

Query: 301 STAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVV 360
           STAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVV
Sbjct: 301 STAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVV 360

Query: 361 NNDYTHWVMYAIGGSANPTINSQGNRYLAPVNPFAKEVTKRVVTSNGVWRHWNWRSEGDL 420
           NNDYTHWVMYAIGGSANPTINSQGNRYLAPVNPFAKEVTKRVVTSNGVWRHWNWRSEGDL
Sbjct: 361 NNDYTHWVMYAIGGSANPTINSQGNRYLAPVNPFAKEVTKRVVTSNGVWRHWNWRSEGDL 420

Query: 421 MLNGAYFTPSGAGAGASYARASSLGAKSSSLVGSLTSNA 460
           MLNGAYFTPSGAGAGASYARASSLGAKSSSLVGSLTSNA
Sbjct: 421 MLNGAYFTPSGAGAGASYARASSLGAKSSSLVGSLTSNA 441

BLAST of Carg05405 vs. TAIR 10
Match: AT5G48900.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 688.3 bits (1775), Expect = 4.1e-198
Identity = 322/374 (86.10%), Postives = 345/374 (92.25%), Query Frame = 0

Query: 86  NEHAVDNPEEIASMVDLSIRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKRLANCG 145
           +EHAV+NPEE+A+MVD+SIRNST RR LG+FSC TGNPIDDCWRCD RWQ RRK LANC 
Sbjct: 33  HEHAVENPEEVAAMVDMSIRNSTARRRLGYFSCSTGNPIDDCWRCDRRWQSRRKHLANCA 92

Query: 146 IGFGRNAVGGRDGRYYVVSDPRDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQEL 205
           IGFGRNA+GGRDGRYYVVSDP DD+PVNP+PGTLRHAVIQ+ PLWIVFKRDMVITLK+EL
Sbjct: 93  IGFGRNAIGGRDGRYYVVSDPNDDNPVNPKPGTLRHAVIQEEPLWIVFKRDMVITLKEEL 152

Query: 206 IMNSFKTIDGRGVNVHIAYGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRT 265
           IMNSFKTIDGRGVNVHIA GACITIQF+TN+IIHG+HIHDC+PTGNAMVRSSPSHYGWRT
Sbjct: 153 IMNSFKTIDGRGVNVHIANGACITIQFVTNIIIHGIHIHDCRPTGNAMVRSSPSHYGWRT 212

Query: 266 MADGDGISIFGSSHIWIDHNSLSSCSDGLIDAIMGSTAITISNNYFTHHNEVMLLGHSDS 325
           MADGDGISIFGSSHIWIDHNSLS+C+DGLIDA+M STAITISNNYFTHHNEVMLLGHSD+
Sbjct: 213 MADGDGISIFGSSHIWIDHNSLSNCADGLIDAVMASTAITISNNYFTHHNEVMLLGHSDT 272

Query: 326 YVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSANPTINSQGN 385
           Y RDK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHW MYAIGGSA+PTINSQGN
Sbjct: 273 YTRDKVMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTINSQGN 332

Query: 386 RYLAPVNPFAKEVTKRVVTSNGVWRHWNWRSEGDLMLNGAYFTPSGAGAGASYARASSLG 445
           RYLAP N FAKEVTKR       WRHWNWRSEGDL LNGA+FT SG+G GASYARASSL 
Sbjct: 333 RYLAPRNRFAKEVTKRDYAGQWQWRHWNWRSEGDLFLNGAFFTRSGSGLGASYARASSLA 392

Query: 446 AKSSSLVGSLTSNA 460
           AKSSSLVG +T NA
Sbjct: 393 AKSSSLVGVITYNA 406

BLAST of Carg05405 vs. TAIR 10
Match: AT3G07010.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 687.2 bits (1772), Expect = 9.1e-198
Identity = 315/372 (84.68%), Postives = 349/372 (93.82%), Query Frame = 0

Query: 86  NEHAVDNPEEIASMVDLSIRNSTERRNLGFFSCGTGNPIDDCWRCDPRWQLRRKRLANCG 145
           +EHAV+NP+E+A+MVD+SIRNSTERR LG+FSC TGNPIDDCWRCD +WQLRRKRLA+C 
Sbjct: 32  HEHAVENPDEVAAMVDMSIRNSTERRRLGYFSCATGNPIDDCWRCDRKWQLRRKRLADCS 91

Query: 146 IGFGRNAVGGRDGRYYVVSDPRDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQEL 205
           IGFGRNA+GGRDGR+YVV+DP DDDPVNP PGTLRHAVIQD PLWI+FKRDMVITLKQEL
Sbjct: 92  IGFGRNAIGGRDGRFYVVTDPGDDDPVNPIPGTLRHAVIQDEPLWIIFKRDMVITLKQEL 151

Query: 206 IMNSFKTIDGRGVNVHIAYGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRT 265
           IMNSFKTIDGRGVNVHIA GAC+TIQ++TN+I+HG+H+HDCKPTGNAMVRSSPSHYG+R+
Sbjct: 152 IMNSFKTIDGRGVNVHIANGACLTIQYVTNIIVHGIHVHDCKPTGNAMVRSSPSHYGFRS 211

Query: 266 MADGDGISIFGSSHIWIDHNSLSSCSDGLIDAIMGSTAITISNNYFTHHNEVMLLGHSDS 325
           MADGD ISIFGSSHIWIDHNSLS+C+DGL+DA+M STAIT+SNN+FTHHNEVMLLGHSDS
Sbjct: 212 MADGDAISIFGSSHIWIDHNSLSNCADGLVDAVMSSTAITVSNNFFTHHNEVMLLGHSDS 271

Query: 326 YVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSANPTINSQGN 385
           Y RDK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHW MYAIGGSA PTINSQGN
Sbjct: 272 YTRDKVMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAGPTINSQGN 331

Query: 386 RYLAPVNPFAKEVTKRVVTSNGVWRHWNWRSEGDLMLNGAYFTPSGAGAGASYARASSLG 445
           R+LAPVNPFAKEVTKR  T    W+HWNWRSEGDL LNGA+FT SGAGAGA+YARASSL 
Sbjct: 332 RFLAPVNPFAKEVTKREYTGESKWKHWNWRSEGDLFLNGAFFTRSGAGAGANYARASSLS 391

Query: 446 AKSSSLVGSLTS 458
           AKSSSLVG++TS
Sbjct: 392 AKSSSLVGTMTS 403

BLAST of Carg05405 vs. TAIR 10
Match: AT3G24670.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 676.8 bits (1745), Expect = 1.2e-194
Identity = 328/405 (80.99%), Postives = 355/405 (87.65%), Query Frame = 0

Query: 57  NSSSMADRFAGDQRLQSFNSSSMADRYVK-----NEHAVDNPEEIASMVDLSIRNSTERR 116
           NSS+MA     D    S ++SS A++        NEHAV NPEE+A+MVD+ I+NSTERR
Sbjct: 23  NSSTMAQE-TEDLNSHSSSNSSTANKLPNDDGAWNEHAVKNPEEVAAMVDMKIKNSTERR 82

Query: 117 NLGFFSCGTGNPIDDCWRCDPRWQLRRKRLANCGIGFGRNAVGGRDGRYYVVSDPRDDDP 176
            LGFFSC TGNPIDDCWRCD  W LRRKRLANC IGFGRNA+GGRDGRYYVV+DP D D 
Sbjct: 83  RLGFFSCATGNPIDDCWRCDRNWHLRRKRLANCAIGFGRNAIGGRDGRYYVVTDPSDHDA 142

Query: 177 VNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQELIMNSFKTIDGRGVNVHIAYGACITIQ 236
           VNPRPGTLRHAVIQDRPLWIVFKRDMVITL QELIMNSFKTIDGRGVNV IA GACITIQ
Sbjct: 143 VNPRPGTLRHAVIQDRPLWIVFKRDMVITLTQELIMNSFKTIDGRGVNVAIAGGACITIQ 202

Query: 237 FITNVIIHGLHIHDCKPTGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWIDHNSLSSCS 296
           ++TN+IIHG+++HDC+ TGNAMVRSSPSHYGWRTMADGD ISIFGSSHIWIDHNSLS+C+
Sbjct: 203 YVTNIIIHGINVHDCRRTGNAMVRSSPSHYGWRTMADGDAISIFGSSHIWIDHNSLSNCA 262

Query: 297 DGLIDAIMGSTAITISNNYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPR 356
           DGLIDAIMGSTAITISNNY THHNEVML+GHSDSY RDK MQVTIAYNHFGEGLIQRMPR
Sbjct: 263 DGLIDAIMGSTAITISNNYMTHHNEVMLMGHSDSYTRDKLMQVTIAYNHFGEGLIQRMPR 322

Query: 357 CRHGYFHVVNNDYTHWVMYAIGGSANPTINSQGNRYLAPVNPFAKEVTKRVVTSNGVWRH 416
           CRHGYFHVVNNDYTHWVMYAIGGSANPTINSQGNR+LAP NPFAKEVTKRV +  G W+ 
Sbjct: 323 CRHGYFHVVNNDYTHWVMYAIGGSANPTINSQGNRFLAPGNPFAKEVTKRVGSWQGEWKQ 382

Query: 417 WNWRSEGDLMLNGAYFTPSGAGAGASYARASSLGAKSSSLVGSLT 457
           WNWRS+GDLMLNGAYFT SGA A ASYARASSLGAK +S+V  LT
Sbjct: 383 WNWRSQGDLMLNGAYFTKSGAAAPASYARASSLGAKPASVVSMLT 426

BLAST of Carg05405 vs. TAIR 10
Match: AT1G04680.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 665.2 bits (1715), Expect = 3.7e-191
Identity = 312/391 (79.80%), Postives = 345/391 (88.24%), Query Frame = 0

Query: 73  SFNSSSMADRYVKNEHAVDNPEEIA----SMVDLSIRNSTERRNLGFFSCGTGNPIDDCW 132
           + +S   +D    N+HAV NP+E+A    ++ ++S+RN TERR LG+F+CGTGNPIDDCW
Sbjct: 28  NISSLPRSDETEWNQHAVTNPDEVADEVLALTEMSVRNHTERRKLGYFTCGTGNPIDDCW 87

Query: 133 RCDPRWQLRRKRLANCGIGFGRNAVGGRDGRYYVVSDPRDDDPVNPRPGTLRHAVIQDRP 192
           RCDP W   RKRLA+CGIGFGRNA+GGRDGR+YVV+DPRDD+PVNPRPGTLRHAVIQDRP
Sbjct: 88  RCDPNWHKNRKRLADCGIGFGRNAIGGRDGRFYVVTDPRDDNPVNPRPGTLRHAVIQDRP 147

Query: 193 LWIVFKRDMVITLKQELIMNSFKTIDGRGVNVHIAYGACITIQFITNVIIHGLHIHDCKP 252
           LWIVFKRDMVI LKQELI+NSFKTIDGRG NVHIA G CITIQF+TNVI+HGLHIHDCKP
Sbjct: 148 LWIVFKRDMVIQLKQELIVNSFKTIDGRGANVHIANGGCITIQFVTNVIVHGLHIHDCKP 207

Query: 253 TGNAMVRSSPSHYGWRTMADGDGISIFGSSHIWIDHNSLSSCSDGLIDAIMGSTAITISN 312
           TGNAMVRSS +H+GWRTMADGD ISIFGSSH+WIDHNSLS C+DGL+DA+MGSTAITISN
Sbjct: 208 TGNAMVRSSETHFGWRTMADGDAISIFGSSHVWIDHNSLSHCADGLVDAVMGSTAITISN 267

Query: 313 NYFTHHNEVMLLGHSDSYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWV 372
           N+ THHNEVMLLGHSDSY+RDK MQVTIAYNHFG GLIQRMPRCRHGYFHVVNNDYTHW 
Sbjct: 268 NHLTHHNEVMLLGHSDSYMRDKAMQVTIAYNHFGVGLIQRMPRCRHGYFHVVNNDYTHWE 327

Query: 373 MYAIGGSANPTINSQGNRYLAPVNPFAKEVTKRVVTSNGVWRHWNWRSEGDLMLNGAYFT 432
           MYAIGGSANPTINSQGNRY AP NPFAKEVTKRV T    W+ WNWRSEGDL+ NGAYFT
Sbjct: 328 MYAIGGSANPTINSQGNRYAAPKNPFAKEVTKRVDTPASHWKGWNWRSEGDLLQNGAYFT 387

Query: 433 PSGAGAGASYARASSLGAKSSSLVGSLTSNA 460
            SGA A  SYARASSL AKSSSLVG +TS+A
Sbjct: 388 SSGAAASGSYARASSLSAKSSSLVGHITSDA 418

BLAST of Carg05405 vs. TAIR 10
Match: AT4G13210.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 664.8 bits (1714), Expect = 4.9e-191
Identity = 315/375 (84.00%), Postives = 341/375 (90.93%), Query Frame = 0

Query: 86  NEHAVDNPEEIASMVDLSIRNSTERRNLGFF-SCGTGNPIDDCWRCDPRWQLRRKRLANC 145
           +EHAV +PEEIA+MVD+SIRNST RR LGFF SC TGNPIDDCWRCD +W  RRKRLA+C
Sbjct: 16  HEHAVKDPEEIAAMVDMSIRNSTYRRKLGFFSSCSTGNPIDDCWRCDKKWHRRRKRLADC 75

Query: 146 GIGFGRNAVGGRDGRYYVVSDPRDDDPVNPRPGTLRHAVIQDRPLWIVFKRDMVITLKQE 205
            IGFGRNAVGGRDGRYY+V+DP D DPV P+PGTLR+AVIQD PLWIVFKRDMVITL QE
Sbjct: 76  AIGFGRNAVGGRDGRYYIVTDPSDHDPVTPKPGTLRYAVIQDEPLWIVFKRDMVITLSQE 135

Query: 206 LIMNSFKTIDGRGVNVHIAYGACITIQFITNVIIHGLHIHDCKPTGNAMVRSSPSHYGWR 265
           LIMNSFKTIDGRGVNVHIA GAC+T+Q++TN+IIHG++IHDCK TGNAMVRSS SHYGWR
Sbjct: 136 LIMNSFKTIDGRGVNVHIAGGACLTVQYVTNIIIHGINIHDCKRTGNAMVRSSESHYGWR 195

Query: 266 TMADGDGISIFGSSHIWIDHNSLSSCSDGLIDAIMGSTAITISNNYFTHHNEVMLLGHSD 325
           TMADGDGISIFGSSHIWIDHNSLSSC+DGLIDAIMGSTAITISNNY THHNE +LLGH+D
Sbjct: 196 TMADGDGISIFGSSHIWIDHNSLSSCADGLIDAIMGSTAITISNNYLTHHNEAILLGHTD 255

Query: 326 SYVRDKQMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWVMYAIGGSANPTINSQG 385
           SY RDK MQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHW MYAIGGSANPTINSQG
Sbjct: 256 SYTRDKMMQVTIAYNHFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQG 315

Query: 386 NRYLAPVNPFAKEVTKRVVTSNGVWRHWNWRSEGDLMLNGAYFTPSGAGAGASYARASSL 445
           NR+LAP N FAKEVTKRV    G W +WNWRS+GDLMLNGAYFT SGAGA A+YARASSL
Sbjct: 316 NRFLAPGNRFAKEVTKRVGAGKGEWNNWNWRSQGDLMLNGAYFTSSGAGASANYARASSL 375

Query: 446 GAKSSSLVGSLTSNA 460
            AKSSSLVG LTS++
Sbjct: 376 AAKSSSLVGMLTSSS 390

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7011311.12.5e-266100.00putative pectate lyase 8, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022963623.12.5e-266100.00probable pectate lyase 8 isoform X2 [Cucurbita moschata][more]
XP_022963622.11.1e-26499.35probable pectate lyase 8 isoform X1 [Cucurbita moschata][more]
XP_023553696.17.6e-26398.47probable pectate lyase 8 isoform X1 [Cucurbita pepo subsp. pepo][more]
KAG6571569.11.0e-259100.00putative pectate lyase 15, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q93WF15.8e-19786.10Probable pectate lyase 20 OS=Arabidopsis thaliana OX=3702 GN=At5g48900 PE=2 SV=1[more]
Q9M8Z81.3e-19684.68Probable pectate lyase 8 OS=Arabidopsis thaliana OX=3702 GN=At3g07010 PE=2 SV=1[more]
Q9LJ421.7e-19380.99Probable pectate lyase 10 OS=Arabidopsis thaliana OX=3702 GN=At3g24670 PE=2 SV=2[more]
Q940Q15.2e-19079.80Probable pectate lyase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g04680 PE=2 SV=2[more]
Q9SVQ66.8e-19084.00Putative pectate lyase 14 OS=Arabidopsis thaliana OX=3702 GN=At4g13210 PE=3 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1HIF31.2e-266100.00Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111463899 PE=3 SV=1[more]
A0A6J1HFR05.1e-26599.35Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111463899 PE=3 SV=1[more]
A0A6J1HKQ95.3e-25495.86Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111463899 PE=3 SV=1[more]
A0A6J1HII49.1e-25496.08Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111463899 PE=3 SV=1[more]
A0A6J1HGK69.1e-25496.08Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111463899 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G48900.14.1e-19886.10Pectin lyase-like superfamily protein [more]
AT3G07010.19.1e-19884.68Pectin lyase-like superfamily protein [more]
AT3G24670.11.2e-19480.99Pectin lyase-like superfamily protein [more]
AT1G04680.13.7e-19179.80Pectin lyase-like superfamily protein [more]
AT4G13210.14.9e-19184.00Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018082AmbAllergenPRINTSPR00807AMBALLERGENcoord: 204..220
score: 70.31
coord: 412..436
score: 54.86
coord: 441..459
score: 28.57
coord: 370..389
score: 75.71
coord: 168..193
score: 69.96
coord: 268..289
score: 68.61
coord: 144..161
score: 53.7
coord: 348..367
score: 78.1
IPR002022Pectate lyaseSMARTSM00656amb_allcoord: 196..393
e-value: 8.1E-102
score: 354.2
IPR002022Pectate lyasePFAMPF00544Pectate_lyase_4coord: 205..385
e-value: 3.7E-19
score: 69.1
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 121..459
e-value: 4.0E-113
score: 379.9
NoneNo IPR availablePANTHERPTHR31683:SF81PECTATE LYASEcoord: 79..459
IPR045032Pectin lyase familyPANTHERPTHR31683PECTATE LYASE 18-RELATEDcoord: 79..459
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 99..439

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg05405-RACarg05405-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045490 pectin catabolic process
molecular_function GO:0046872 metal ion binding
molecular_function GO:0030570 pectate lyase activity