Carg05293 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg05293
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionRING-type E3 ubiquitin transferase
LocationCarg_Chr07: 4026834 .. 4027496 (-)
RNA-Seq ExpressionCarg05293
SyntenyCarg05293
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTATCTCCATTACAAGAAGGTTCCTCCTCCCCACCGCCACCGCCACCGCCACCGCCACCGCTGTCGCCACTCCGTCTTCGCCACCGCAGCCCCGCCCTGGGTTCGGTAGCTTCGACTTGAACGTTGTAATGGTCCTATCCGTCCTCCTCTGCGCTCTAGTTTGCTCTTTGGGTCTCAACGCAATCCTCAAATGCGCCTTGCGGTGCTCCACTTTGCTGGCCTCCGCGTCGGCTCGCCACGGCGGGAATATAGCGATCCACCCGAAAGGCGTTGGACGAAATACGTTAAAGAAATTCCCGACGGTGGAGTACTCAAAACAGAGCAATAAATTCAGGGGAATCGCAATGGATGGGGAGTGCGTGATTTGCTTGGCGGATTTCGAGCCGGGCGATCGGGTTCGTATTTTGCCCAAATGCTACCATGGGTTCCATGTTCATTGCATCGATAAGTGGCTGAGTTCCCACACTTCTTGCCCCAAGTGCCGTAATTGCCTCTCTGATACTTCCCGGAAGGTCGACGCCGGTTGTGGGTCAGCGGTGGAGGGGAGTTCGTCCTCTGTTGGGCCGCCGCCGCCCTCGGAGGAGGTGGTGGTGAATGTTGGTATTGCTCCGGTGGAACGGGAAGGTCTGACGTGTAGCTATAGAGACAGTAGTAGATGA

mRNA sequence

ATGTCTATCTCCATTACAAGAAGGTTCCTCCTCCCCACCGCCACCGCCACCGCCACCGCCACCGCTGTCGCCACTCCGTCTTCGCCACCGCAGCCCCGCCCTGGGTTCGGTAGCTTCGACTTGAACGTTGTAATGGTCCTATCCGTCCTCCTCTGCGCTCTAGTTTGCTCTTTGGGTCTCAACGCAATCCTCAAATGCGCCTTGCGGTGCTCCACTTTGCTGGCCTCCGCGTCGGCTCGCCACGGCGGGAATATAGCGATCCACCCGAAAGGCGTTGGACGAAATACGTTAAAGAAATTCCCGACGGTGGAGTACTCAAAACAGAGCAATAAATTCAGGGGAATCGCAATGGATGGGGAGTGCGTGATTTGCTTGGCGGATTTCGAGCCGGGCGATCGGGTTCGTATTTTGCCCAAATGCTACCATGGGTTCCATGTTCATTGCATCGATAAGTGGCTGAGTTCCCACACTTCTTGCCCCAAGTGCCGTAATTGCCTCTCTGATACTTCCCGGAAGGTCGACGCCGGTTGTGGGTCAGCGGTGGAGGGGAGTTCGTCCTCTGTTGGGCCGCCGCCGCCCTCGGAGGAGGTGGTGGTGAATGTTGGTATTGCTCCGGTGGAACGGGAAGGTCTGACGTGTAGCTATAGAGACAGTAGTAGATGA

Coding sequence (CDS)

ATGTCTATCTCCATTACAAGAAGGTTCCTCCTCCCCACCGCCACCGCCACCGCCACCGCCACCGCTGTCGCCACTCCGTCTTCGCCACCGCAGCCCCGCCCTGGGTTCGGTAGCTTCGACTTGAACGTTGTAATGGTCCTATCCGTCCTCCTCTGCGCTCTAGTTTGCTCTTTGGGTCTCAACGCAATCCTCAAATGCGCCTTGCGGTGCTCCACTTTGCTGGCCTCCGCGTCGGCTCGCCACGGCGGGAATATAGCGATCCACCCGAAAGGCGTTGGACGAAATACGTTAAAGAAATTCCCGACGGTGGAGTACTCAAAACAGAGCAATAAATTCAGGGGAATCGCAATGGATGGGGAGTGCGTGATTTGCTTGGCGGATTTCGAGCCGGGCGATCGGGTTCGTATTTTGCCCAAATGCTACCATGGGTTCCATGTTCATTGCATCGATAAGTGGCTGAGTTCCCACACTTCTTGCCCCAAGTGCCGTAATTGCCTCTCTGATACTTCCCGGAAGGTCGACGCCGGTTGTGGGTCAGCGGTGGAGGGGAGTTCGTCCTCTGTTGGGCCGCCGCCGCCCTCGGAGGAGGTGGTGGTGAATGTTGGTATTGCTCCGGTGGAACGGGAAGGTCTGACGTGTAGCTATAGAGACAGTAGTAGATGA

Protein sequence

MSISITRRFLLPTATATATATAVATPSSPPQPRPGFGSFDLNVVMVLSVLLCALVCSLGLNAILKCALRCSTLLASASARHGGNIAIHPKGVGRNTLKKFPTVEYSKQSNKFRGIAMDGECVICLADFEPGDRVRILPKCYHGFHVHCIDKWLSSHTSCPKCRNCLSDTSRKVDAGCGSAVEGSSSSVGPPPPSEEVVVNVGIAPVEREGLTCSYRDSSR
Homology
[Close]
BLAST of Carg05293 vs. NCBI nr
Match: KAG7027130.1 (RING-H2 finger protein ATL78, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 442.2 bits (1136), Expect = 2.6e-120
Identity = 220/220 (100.00%), Postives = 220/220 (100.00%), Query Frame = 0 

Query: 1   MSISITRRFLLPTATATATATAVATPSSPPQPRPGFGSFDLNVVMVLSVLLCALVCSLGL 60
           MSISITRRFLLPTATATATATAVATPSSPPQPRPGFGSFDLNVVMVLSVLLCALVCSLGL
Sbjct: 1   MSISITRRFLLPTATATATATAVATPSSPPQPRPGFGSFDLNVVMVLSVLLCALVCSLGL 60

Query: 61  NAILKCALRCSTLLASASARHGGNIAIHPKGVGRNTLKKFPTVEYSKQSNKFRGIAMDGE 120
           NAILKCALRCSTLLASASARHGGNIAIHPKGVGRNTLKKFPTVEYSKQSNKFRGIAMDGE
Sbjct: 61  NAILKCALRCSTLLASASARHGGNIAIHPKGVGRNTLKKFPTVEYSKQSNKFRGIAMDGE 120

Query: 121 CVICLADFEPGDRVRILPKCYHGFHVHCIDKWLSSHTSCPKCRNCLSDTSRKVDAGCGSA 180
           CVICLADFEPGDRVRILPKCYHGFHVHCIDKWLSSHTSCPKCRNCLSDTSRKVDAGCGSA
Sbjct: 121 CVICLADFEPGDRVRILPKCYHGFHVHCIDKWLSSHTSCPKCRNCLSDTSRKVDAGCGSA 180

Query: 181 VEGSSSSVGPPPPSEEVVVNVGIAPVEREGLTCSYRDSSR 221
           VEGSSSSVGPPPPSEEVVVNVGIAPVEREGLTCSYRDSSR
Sbjct: 181 VEGSSSSVGPPPPSEEVVVNVGIAPVEREGLTCSYRDSSR 220

[Close]
BLAST of Carg05293 vs. NCBI nr
Match: XP_022963109.1 (RING-H2 finger protein ATL74-like [Cucurbita moschata])

HSP 1 Score: 417.5 bits (1072), Expect = 6.9e-113
Identity = 213/222 (95.95%), Postives = 213/222 (95.95%), Query Frame = 0 

Query: 1   MSISITRRFLLPTATATATATAVATPSSPPQPRPGFGSFDLNVVMVLSVLLCALVCSLGL 60
           MSISITRRFLLP    TATATAVATPSSPPQPRPGFGSFDLNVVMVLSVLLCALV SLGL
Sbjct: 1   MSISITRRFLLP----TATATAVATPSSPPQPRPGFGSFDLNVVMVLSVLLCALVFSLGL 60

Query: 61  NAILKCALRCSTLLASASARHGGNIAIHPKGVGRNTLKKFPTVEYSKQSNKFRGIAMDGE 120
           NAILKCALRCSTLLASASARHGGNIAIHPKGVGRNTLKKFPTVEYSKQSNKFRGIAMDGE
Sbjct: 61  NAILKCALRCSTLLASASARHGGNIAIHPKGVGRNTLKKFPTVEYSKQSNKFRGIAMDGE 120

Query: 121 CVICLADFEPGDRVRILPKCYHGFHVHCIDKWLSSHTSCPKCRNCLSDTSRKVDAGCGSA 180
           CVICLADFEPGDRVRILPKCYHGFHVHCIDKWLSSHTSCPKCRNCLSDTSRKVDAGCGSA
Sbjct: 121 CVICLADFEPGDRVRILPKCYHGFHVHCIDKWLSSHTSCPKCRNCLSDTSRKVDAGCGSA 180

Query: 181 VEGSSSSVG--PPPPSEEVVVNVGIAPVEREGLTCSYRDSSR 221
           VEGSSSSVG  PPPPSEEVVVNVGIAPVEREGL CSYRDS R
Sbjct: 181 VEGSSSSVGPPPPPPSEEVVVNVGIAPVEREGLRCSYRDSGR 218

[Close]
BLAST of Carg05293 vs. NCBI nr
Match: XP_023518448.1 (RING-H2 finger protein ATL78-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 416.4 bits (1069), Expect = 1.5e-112
Identity = 207/220 (94.09%), Postives = 213/220 (96.82%), Query Frame = 0 

Query: 1   MSISITRRFLLPTATATATATAVATPSSPPQPRPGFGSFDLNVVMVLSVLLCALVCSLGL 60
           MSIS +RRFLLP    +ATATAVATPSS PQPRPGFGSFD+NVVMVLSVLLCALVCSLGL
Sbjct: 1   MSISTSRRFLLP----SATATAVATPSSLPQPRPGFGSFDMNVVMVLSVLLCALVCSLGL 60

Query: 61  NAILKCALRCSTLLASASARHGGNIAIHPKGVGRNTLKKFPTVEYSKQSNKFRGIAMDGE 120
           NAILKCALRCSTLLASASARHGGN+AIHPKGVGRN LKKFPTVEYSKQSNKFRGIAMDGE
Sbjct: 61  NAILKCALRCSTLLASASARHGGNLAIHPKGVGRNILKKFPTVEYSKQSNKFRGIAMDGE 120

Query: 121 CVICLADFEPGDRVRILPKCYHGFHVHCIDKWLSSHTSCPKCRNCLSDTSRKVDAGCGSA 180
           CVICLADFEPGDRVR+LPKCYHGFHVHCIDKWLSSHTSCPKCRNCLSDTSRKVDAGCGSA
Sbjct: 121 CVICLADFEPGDRVRVLPKCYHGFHVHCIDKWLSSHTSCPKCRNCLSDTSRKVDAGCGSA 180

Query: 181 VEGSSSSVGPPPPSEEVVVNVGIAPVEREGLTCSYRDSSR 221
           VEGSSSSVGPPPPSEEV+VNVGIAPVEREGLTCSYRDSSR
Sbjct: 181 VEGSSSSVGPPPPSEEVLVNVGIAPVEREGLTCSYRDSSR 216

[Close]
BLAST of Carg05293 vs. NCBI nr
Match: KAG6595117.1 (RING-H2 finger protein ATL78, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 399.4 bits (1025), Expect = 2.0e-107
Identity = 203/213 (95.31%), Postives = 203/213 (95.31%), Query Frame = 0 

Query: 1   MSISITRRFLLPTATATATATAVATPSSPPQPRPGFGSFDLNVVMVLSVLLCALVCSLGL 60
           MSISITRRFLLP      TATAVATPSSPPQPRPGFGSFDLNVVMVLSVLLCALVCSLGL
Sbjct: 1   MSISITRRFLLP------TATAVATPSSPPQPRPGFGSFDLNVVMVLSVLLCALVCSLGL 60

Query: 61  NAILKCALRCSTLLASASARHGGNIAIHPKGVGRNTLKKFPTVEYSKQSNKFRGIAMDGE 120
           NAILKCALRCST LASASARHGGNIAIHPKGVGRNTLKKFPTVEYSKQSNKFRGIAMDGE
Sbjct: 61  NAILKCALRCSTFLASASARHGGNIAIHPKGVGRNTLKKFPTVEYSKQSNKFRGIAMDGE 120

Query: 121 CVICLADFEPGDRVRILPKCYHGFHVHCIDKWLSSHTSCPKCRNCLSDTSRKVDAGCGSA 180
           CVICLADFEPGDRVRILPKCYHGFHVHCIDKWLSSHTSCPKCRNCLSDTSRKVD GCGSA
Sbjct: 121 CVICLADFEPGDRVRILPKCYHGFHVHCIDKWLSSHTSCPKCRNCLSDTSRKVDGGCGSA 180

Query: 181 VEGSSSSVG-PPPPSEEVVVNVGIAPVEREGLT 213
           VEGSS SVG PPPPSEEVVVNVGIAPVEREGLT
Sbjct: 181 VEGSSYSVGPPPPPSEEVVVNVGIAPVEREGLT 207

[Close]
BLAST of Carg05293 vs. NCBI nr
Match: XP_038882744.1 (RING-H2 finger protein ATL78 [Benincasa hispida])

HSP 1 Score: 303.9 bits (777), Expect = 1.1e-78
Identity = 162/226 (71.68%), Postives = 180/226 (79.65%), Query Frame = 0 

Query: 1   MSISITRRFLLPTATATATATAVATPSSPPQPRPGFGSFDLNVVMVLSVLLCALVCSLGL 60
           MSIS +RR LLP      +ATA A+P    QPRPGFGSFD+NVVMVLSVLLCAL+CSLGL
Sbjct: 1   MSISNSRRLLLP------SATAAASPPELAQPRPGFGSFDMNVVMVLSVLLCALICSLGL 60

Query: 61  NAILKCALRCSTLLASAS-ARHGGNIAIHPKGVGRNTLKKFPTVEYSKQSNKFRGIAMDG 120
           NAILKCALRCSTLLA+ S  R GG + +HPKGV RN LKKFPTVEYSK+ NK RGI  DG
Sbjct: 61  NAILKCALRCSTLLATVSGGRGGGALVVHPKGVRRNILKKFPTVEYSKEGNKLRGI--DG 120

Query: 121 ECVICLADFEPGDRVRILPKCYHGFHVHCIDKWLSSHTSCPKCRNCLSDTSRKVDAGCG- 180
           ECVICL +FE GDRVR+LPKCYHGFHVHCIDKWLSSHTSCPKCRNCL+DT  K+ AGCG 
Sbjct: 121 ECVICLLEFEAGDRVRVLPKCYHGFHVHCIDKWLSSHTSCPKCRNCLTDTCPKITAGCGQ 180

Query: 181 ------SAVEGSSSSVGPPPPSEEVVVNVGIAPVEREGLTCSYRDS 219
                 +A+  SSSSV  PP +EEV VNV IAPVEREGL  +YR+S
Sbjct: 181 EAPITTAALAESSSSV-DPPAAEEVGVNVVIAPVEREGLISNYRES 217

[Close]
BLAST of Carg05293 vs. ExPASy Swiss-Prot
Match: Q6NQG7 (RING-H2 finger protein ATL78 OS=Arabidopsis thaliana OX=3702 GN=ATL78 PE=2 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 1.5e-41
Identity = 92/185 (49.73%), Postives = 119/185 (64.32%), Query Frame = 0 

Query: 25  TPSSPPQPRPGFGSFDLNVVMVLSVLLCALVCSLGLNAILKCALRCSTLLASASARHGGN 84
           +P+  P P  G  +FD NVVMVLSVLLCALVCSLGLN+I++CALRCS L+ S +      
Sbjct: 38  SPTPAPSPYVGDNNFDANVVMVLSVLLCALVCSLGLNSIIRCALRCSNLVPSEAGGDNYP 97

Query: 85  IAIHPKGVGRNTLKKFPTVEYSKQSNKFRGIAMDGECVICLADFEPGDRVRILPKCYHGF 144
           + +   GV R  LK F TV YS + N      +D EC ICL++F   +RV++LP C+HGF
Sbjct: 98  VRLTNTGVKRKALKSFQTVSYSTELNL---PGLDTECAICLSEFVAEERVKLLPTCHHGF 157

Query: 145 HVHCIDKWLSSHTSCPKCRNCLSDTSRKVDAGCGSAVEGSSSSVGPPPPSEEVVVNVGIA 204
           HV CIDKWLSSH+SCP CR+CL  T  K+ A C      +SS     PP + +++   IA
Sbjct: 158 HVRCIDKWLSSHSSCPTCRHCLIQTCEKI-ADCSQ----TSSLNSTQPPQDSIILQ--IA 212

Query: 205 PVERE 210
           P+E E
Sbjct: 218 PLEPE 212

[Close]
BLAST of Carg05293 vs. ExPASy Swiss-Prot
Match: Q6NML0 (E3 ubiquitin-protein ligase ATL76 OS=Arabidopsis thaliana OX=3702 GN=ATL76 PE=1 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 9.6e-33
Identity = 80/185 (43.24%), Postives = 117/185 (63.24%), Query Frame = 0 

Query: 42  NVVMVLSVLLCALVCSLGLNAILKCALRCST-------LLASASARHGGNIAIHPKGVGR 101
           NV+M+LSVL+C ++C LGL+ I++CA R S+       + + ++ R   N     KG+ +
Sbjct: 57  NVLMLLSVLICGIICCLGLHYIIRCAFRRSSRFMISEPISSLSTPRSSSN-----KGIKK 116

Query: 102 NTLKKFPTVEYSKQSNKFRGIAMDGECVICLADFEPGDRVRILPKCYHGFHVHCIDKWLS 161
             L+ FP V YS++ N   GI    ECVICL+DF  G+++R+LPKC HGFHV CIDKWL 
Sbjct: 117 KALRMFPVVSYSREMN-LPGIG--EECVICLSDFVSGEQLRLLPKCNHGFHVRCIDKWLQ 176

Query: 162 SHTSCPKCRNCLSDTSRKVDAGCGSAVEGSSSSVGPPPPSEEVVVNVGIAPVEREGLTCS 220
            H +CPKCR+CL +T +K+  G  S  +  +S+     P+E V+V   I P+E EG   +
Sbjct: 177 HHLTCPKCRHCLVETCQKI-LGDFSQADSMAST-----PTESVIVR--IDPLEPEGRVNT 225

[Close]
BLAST of Carg05293 vs. ExPASy Swiss-Prot
Match: P0C034 (RING-H2 finger protein ATL10 OS=Arabidopsis thaliana OX=3702 GN=ATL10 PE=2 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 3.7e-32
Identity = 90/239 (37.66%), Postives = 129/239 (53.97%), Query Frame = 0 

Query: 5   ITRRFLLPTATATATATAVATPSSPPQPRPGFGSFDLNVVMVLSVLLCALVCSLGLNAIL 64
           ++R+ LL       T  A A   SP        +   NV+M+LS+L+C ++C LGL+ I+
Sbjct: 22  VSRKLLLHNPFDHNTQRAFAVAPSPLITHE--NNLSGNVMMLLSILICGIICCLGLHYII 81

Query: 65  KCALRCSTL------LASASARHGGNIAIHPKGVGRNTLKKFPTVEYSKQSNKFRGIAMD 124
           +CALR ST       + S S+  G +     KG+ +  L+ FP V YS + N      +D
Sbjct: 82  RCALRRSTRFMISEPVPSLSSTRGSS----NKGIKKKALRMFPVVSYSPEMNL---PGLD 141

Query: 125 GECVICLADFEPGDRVRILPKCYHGFHVHCIDKWLSSHTSCPKCRNCLSDTSRKV----- 184
            ECVICL+DF  G+++R+LPKC HGFHV CIDKWL  H +CPKCRNCL +T +K+     
Sbjct: 142 EECVICLSDFVSGEQLRLLPKCNHGFHVRCIDKWLQQHLTCPKCRNCLVETCQKILGDFS 201

Query: 185 --DAGCGSAVEGSSSSVGPPPPSEEVVVNVG-----------IAPVEREGLTCSYRDSS 220
             D+      E    ++ P  P+E V+V +            IAP+E EG   + R+ S
Sbjct: 202 QADSVTAEPTEIVIVTIVPLEPTEIVIVTIAPLEPTEIVIVMIAPLEPEGRVNTIREIS 251

[Close]
BLAST of Carg05293 vs. ExPASy Swiss-Prot
Match: Q9LS99 (RING-H2 finger protein ATL77 OS=Arabidopsis thaliana OX=3702 GN=ATL77 PE=2 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 1.1e-31
Identity = 74/181 (40.88%), Postives = 115/181 (63.54%), Query Frame = 0 

Query: 42  NVVMVLSVLLCALVCSLGLNAILKCA-LRCSTLLAS--ASARHGGNIAIHPKGVGRNTLK 101
           NV+M+LS+LLC ++CSLGL+ I++CA +R  + + S   S       +   KG+ +  LK
Sbjct: 51  NVLMLLSILLCGIICSLGLHYIIRCAFIRSRSFMISDPISIPSTPRDSSVNKGIKKKALK 110

Query: 102 KFPTVEYSKQSNKFRGIAMDGECVICLADFEPGDRVRILPKCYHGFHVHCIDKWLSSHTS 161
             P V YS + N   G+    ECVICL+DF  G+++R+LPKC HGFH+ CIDKWL+ H +
Sbjct: 111 MLPVVNYSPEIN-LPGVG--EECVICLSDFVAGEQLRVLPKCNHGFHLRCIDKWLTQHMT 170

Query: 162 CPKCRNCLSDTSRKVDAGCGSAVEGSSSSVGPPPPSEEVVVNVGIAPVEREGLTCSYRDS 220
           CPKCR+CL DT +KV + C +A + ++++           +++ I+P+E E    ++R+S
Sbjct: 171 CPKCRHCLVDTCQKVLSDCDAADQVAATATES--------IDIRISPLEPEARVATFRES 220

[Close]
BLAST of Carg05293 vs. ExPASy Swiss-Prot
Match: Q94BY6 (RING-H2 finger protein ATL75 OS=Arabidopsis thaliana OX=3702 GN=ATL75 PE=2 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 2.4e-31
Identity = 83/217 (38.25%), Postives = 122/217 (56.22%), Query Frame = 0 

Query: 5   ITRRFLLPTATATATATAVATPSSPPQPRPGFGSFDLNVVMVLSVLLCALVCSLGLNAIL 64
           ++R+ LL       T  A A   SP        +   NV+M+LSVL+C ++C LGL+ I+
Sbjct: 23  VSRKLLLHNPFDHNTQRAFAVAPSPLITHE--NNLSGNVLMLLSVLICGIICCLGLHYII 82

Query: 65  KCALR--CSTLLASASARHGGNIAIHPKGVGRNTLKKFPTVEYSKQSNKFRGIAMDGECV 124
           +CA R   S +++   A          KG+ +  L+ FP V YS + N      +  ECV
Sbjct: 83  RCAFRRTSSFMISEPIAGLSTPCGSSNKGINKKALRMFPVVSYSPEMNL---PGLGEECV 142

Query: 125 ICLADFEPGDRVRILPKCYHGFHVHCIDKWLSSHTSCPKCRNCLSDTSRKVDAGCGSAVE 184
           ICL+DF  G+++R+LPKC+HGFHV CIDKWL  H +CPKCR+CL +T +K+      A +
Sbjct: 143 ICLSDFVSGEQIRMLPKCHHGFHVRCIDKWLQQHLTCPKCRHCLVETCQKILGDFSQADQ 202

Query: 185 GSSSSVGPPPPSEEVVVNVGIAPVEREGLTCSYRDSS 220
            +++      P+  V+V   IAP+E EG     R+SS
Sbjct: 203 VAAT------PTASVIVR--IAPLEPEGRVNILRESS 226

[Close]
BLAST of Carg05293 vs. ExPASy TrEMBL
Match: A0A6J1HJ58 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111463410 PE=3 SV=1)

HSP 1 Score: 417.5 bits (1072), Expect = 3.4e-113
Identity = 213/222 (95.95%), Postives = 213/222 (95.95%), Query Frame = 0 

Query: 1   MSISITRRFLLPTATATATATAVATPSSPPQPRPGFGSFDLNVVMVLSVLLCALVCSLGL 60
           MSISITRRFLLP    TATATAVATPSSPPQPRPGFGSFDLNVVMVLSVLLCALV SLGL
Sbjct: 1   MSISITRRFLLP----TATATAVATPSSPPQPRPGFGSFDLNVVMVLSVLLCALVFSLGL 60

Query: 61  NAILKCALRCSTLLASASARHGGNIAIHPKGVGRNTLKKFPTVEYSKQSNKFRGIAMDGE 120
           NAILKCALRCSTLLASASARHGGNIAIHPKGVGRNTLKKFPTVEYSKQSNKFRGIAMDGE
Sbjct: 61  NAILKCALRCSTLLASASARHGGNIAIHPKGVGRNTLKKFPTVEYSKQSNKFRGIAMDGE 120

Query: 121 CVICLADFEPGDRVRILPKCYHGFHVHCIDKWLSSHTSCPKCRNCLSDTSRKVDAGCGSA 180
           CVICLADFEPGDRVRILPKCYHGFHVHCIDKWLSSHTSCPKCRNCLSDTSRKVDAGCGSA
Sbjct: 121 CVICLADFEPGDRVRILPKCYHGFHVHCIDKWLSSHTSCPKCRNCLSDTSRKVDAGCGSA 180

Query: 181 VEGSSSSVG--PPPPSEEVVVNVGIAPVEREGLTCSYRDSSR 221
           VEGSSSSVG  PPPPSEEVVVNVGIAPVEREGL CSYRDS R
Sbjct: 181 VEGSSSSVGPPPPPPSEEVVVNVGIAPVEREGLRCSYRDSGR 218

[Close]
BLAST of Carg05293 vs. ExPASy TrEMBL
Match: A0A5A7SLG8 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1230G00380 PE=3 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 9.2e-79
Identity = 159/226 (70.35%), Postives = 177/226 (78.32%), Query Frame = 0 

Query: 1   MSISITRRFLLPTATATATATAVATPSSPPQPRPGFGSFDLNVVMVLSVLLCALVCSLGL 60
           MSIS +RRFLLP      + TA A+P    QPRPGFGSFD+NVVMVLSVLLCAL+CSLGL
Sbjct: 1   MSISNSRRFLLP------SPTAAASPPELAQPRPGFGSFDMNVVMVLSVLLCALICSLGL 60

Query: 61  NAILKCALRCSTLLASAS-ARHGGNIAIHPKGVGRNTLKKFPTVEYSKQSNKFRGIAMDG 120
           NAILKCALRCSTLLA+ S  R GG + +HPKGV RN LKKFPTVEYSK+ NK RGI  DG
Sbjct: 61  NAILKCALRCSTLLATVSGGRGGGALVVHPKGVRRNVLKKFPTVEYSKEGNKLRGI--DG 120

Query: 121 ECVICLADFEPGDRVRILPKCYHGFHVHCIDKWLSSHTSCPKCRNCLSDTSRKVDAGCG- 180
           ECVICL +FE GDRVR+LPKCYHGFHVHCIDKWLSSHTSCPKCRNCL+DT  K+ AGCG 
Sbjct: 121 ECVICLLEFEAGDRVRVLPKCYHGFHVHCIDKWLSSHTSCPKCRNCLTDTCHKIAAGCGQ 180

Query: 181 ------SAVEGSSSSVGPPPPSEEVVVNVGIAPVEREGLTCSYRDS 219
                 +A    SSS   PP +EEV VNV IAPVEREG+  +YR+S
Sbjct: 181 ETPVTTAATAAESSSSVEPPRAEEVEVNVVIAPVEREGMINNYRES 218

[Close]
BLAST of Carg05293 vs. ExPASy TrEMBL
Match: A0A1S3B203 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103485255 PE=3 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 9.2e-79
Identity = 159/226 (70.35%), Postives = 177/226 (78.32%), Query Frame = 0 

Query: 1   MSISITRRFLLPTATATATATAVATPSSPPQPRPGFGSFDLNVVMVLSVLLCALVCSLGL 60
           MSIS +RRFLLP      + TA A+P    QPRPGFGSFD+NVVMVLSVLLCAL+CSLGL
Sbjct: 1   MSISNSRRFLLP------SPTAAASPPELAQPRPGFGSFDMNVVMVLSVLLCALICSLGL 60

Query: 61  NAILKCALRCSTLLASAS-ARHGGNIAIHPKGVGRNTLKKFPTVEYSKQSNKFRGIAMDG 120
           NAILKCALRCSTLLA+ S  R GG + +HPKGV RN LKKFPTVEYSK+ NK RGI  DG
Sbjct: 61  NAILKCALRCSTLLATVSGGRGGGALVVHPKGVRRNVLKKFPTVEYSKEGNKLRGI--DG 120

Query: 121 ECVICLADFEPGDRVRILPKCYHGFHVHCIDKWLSSHTSCPKCRNCLSDTSRKVDAGCG- 180
           ECVICL +FE GDRVR+LPKCYHGFHVHCIDKWLSSHTSCPKCRNCL+DT  K+ AGCG 
Sbjct: 121 ECVICLLEFEAGDRVRVLPKCYHGFHVHCIDKWLSSHTSCPKCRNCLTDTCHKIAAGCGQ 180

Query: 181 ------SAVEGSSSSVGPPPPSEEVVVNVGIAPVEREGLTCSYRDS 219
                 +A    SSS   PP +EEV VNV IAPVEREG+  +YR+S
Sbjct: 181 ETPVTTAATAAESSSSVEPPRAEEVEVNVVIAPVEREGMINNYRES 218

[Close]
BLAST of Carg05293 vs. ExPASy TrEMBL
Match: A0A0A0KF92 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G484580 PE=3 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 3.5e-78
Identity = 157/223 (70.40%), Postives = 174/223 (78.03%), Query Frame = 0 

Query: 1   MSISITRRFLLPTATATATATAVATPSSPPQPRPGFGSFDLNVVMVLSVLLCALVCSLGL 60
           MSIS +RRFL+P      +AT  ATP    QPRPGFGSFD+NVVMVLSVLLCAL+CSLGL
Sbjct: 1   MSISNSRRFLIP------SATVAATPPELAQPRPGFGSFDMNVVMVLSVLLCALICSLGL 60

Query: 61  NAILKCALRCSTLLASAS-ARHGGNIAIHPKGVGRNTLKKFPTVEYSKQSNKFRGIAMDG 120
           NAILKCALRCSTLLA+ S  R GG + +HPKGV RN LKKFPTVEYSK+ NK RGI  DG
Sbjct: 61  NAILKCALRCSTLLATVSGGRGGGALVVHPKGVRRNVLKKFPTVEYSKEGNKLRGI--DG 120

Query: 121 ECVICLADFEPGDRVRILPKCYHGFHVHCIDKWLSSHTSCPKCRNCLSDTSRKVDAGCGS 180
           ECVICL +FE GDRVR+LPKCYHGFHVHCIDKWLSSHTSCPKCRNCL+DT  K+ AGC  
Sbjct: 121 ECVICLLEFEAGDRVRVLPKCYHGFHVHCIDKWLSSHTSCPKCRNCLTDTCHKITAGCAQ 180

Query: 181 ----AVEGSSSSVGPPPPSEEVVVNVGIAPVEREGLTCSYRDS 219
                    SSS   PP +EEV VNV IAPVEREG+  +YR+S
Sbjct: 181 ETHVTTAAESSSSVEPPRAEEVGVNVVIAPVEREGMINNYRES 215

[Close]
BLAST of Carg05293 vs. ExPASy TrEMBL
Match: A0A6J1GCL7 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111452965 PE=3 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 3.1e-74
Identity = 151/221 (68.33%), Postives = 171/221 (77.38%), Query Frame = 0 

Query: 1   MSISITRRFLLPTATATATATAVATPSSPPQPRPGFGSFDLNVVMVLSVLLCALVCSLGL 60
           MSI+ +RR LLP  TA+   +A        QPRPGFGSFD+NVVMVLSVLLCALVCSLGL
Sbjct: 1   MSITNSRRLLLPAVTASPPVSA--------QPRPGFGSFDMNVVMVLSVLLCALVCSLGL 60

Query: 61  NAILKCALRCSTLLASASARHGGNI-AIHPKGVGRNTLKKFPTVEYSKQSNKFRGIAMDG 120
           NA+LKCALRCS+LLA+ S   GG I A+HPKGV RN LKKFPTVEY K+ NK RGI  DG
Sbjct: 61  NALLKCALRCSSLLATMSGGRGGGIFAVHPKGVRRNVLKKFPTVEYPKEGNKLRGI--DG 120

Query: 121 ECVICLADFEPGDRVRILPKCYHGFHVHCIDKWLSSHTSCPKCRNCLSDTSRKV-DAGCG 180
           ECVICL++FE GDRVR+LP C+HGFHVHCIDKWLSSH SCPKCRNCL+DT  K+   G  
Sbjct: 121 ECVICLSEFEGGDRVRVLPICFHGFHVHCIDKWLSSHNSCPKCRNCLTDTCHKIASGGSQ 180

Query: 181 SAVEGSSSSVG---PPPPSEEVVVNVGIAPVEREGLTCSYR 217
            A +G   ++    PPPP EEVVVNV IAPVEREGL C+YR
Sbjct: 181 EAPDGEFIAIDPPPPPPPGEEVVVNVVIAPVEREGLVCNYR 211

[Close]
BLAST of Carg05293 vs. TAIR 10
Match: AT1G49230.1 (RING/U-box superfamily protein )

HSP 1 Score: 171.0 bits (432), Expect = 1.1e-42
Identity = 92/185 (49.73%), Postives = 119/185 (64.32%), Query Frame = 0 

Query: 25  TPSSPPQPRPGFGSFDLNVVMVLSVLLCALVCSLGLNAILKCALRCSTLLASASARHGGN 84
           +P+  P P  G  +FD NVVMVLSVLLCALVCSLGLN+I++CALRCS L+ S +      
Sbjct: 38  SPTPAPSPYVGDNNFDANVVMVLSVLLCALVCSLGLNSIIRCALRCSNLVPSEAGGDNYP 97

Query: 85  IAIHPKGVGRNTLKKFPTVEYSKQSNKFRGIAMDGECVICLADFEPGDRVRILPKCYHGF 144
           + +   GV R  LK F TV YS + N      +D EC ICL++F   +RV++LP C+HGF
Sbjct: 98  VRLTNTGVKRKALKSFQTVSYSTELNL---PGLDTECAICLSEFVAEERVKLLPTCHHGF 157

Query: 145 HVHCIDKWLSSHTSCPKCRNCLSDTSRKVDAGCGSAVEGSSSSVGPPPPSEEVVVNVGIA 204
           HV CIDKWLSSH+SCP CR+CL  T  K+ A C      +SS     PP + +++   IA
Sbjct: 158 HVRCIDKWLSSHSSCPTCRHCLIQTCEKI-ADCSQ----TSSLNSTQPPQDSIILQ--IA 212

Query: 205 PVERE 210
           P+E E
Sbjct: 218 PLEPE 212

[Close]
BLAST of Carg05293 vs. TAIR 10
Match: AT1G49210.1 (RING/U-box superfamily protein )

HSP 1 Score: 141.7 bits (356), Expect = 6.8e-34
Identity = 80/185 (43.24%), Postives = 117/185 (63.24%), Query Frame = 0 

Query: 42  NVVMVLSVLLCALVCSLGLNAILKCALRCST-------LLASASARHGGNIAIHPKGVGR 101
           NV+M+LSVL+C ++C LGL+ I++CA R S+       + + ++ R   N     KG+ +
Sbjct: 57  NVLMLLSVLICGIICCLGLHYIIRCAFRRSSRFMISEPISSLSTPRSSSN-----KGIKK 116

Query: 102 NTLKKFPTVEYSKQSNKFRGIAMDGECVICLADFEPGDRVRILPKCYHGFHVHCIDKWLS 161
             L+ FP V YS++ N   GI    ECVICL+DF  G+++R+LPKC HGFHV CIDKWL 
Sbjct: 117 KALRMFPVVSYSREMN-LPGIG--EECVICLSDFVSGEQLRLLPKCNHGFHVRCIDKWLQ 176

Query: 162 SHTSCPKCRNCLSDTSRKVDAGCGSAVEGSSSSVGPPPPSEEVVVNVGIAPVEREGLTCS 220
            H +CPKCR+CL +T +K+  G  S  +  +S+     P+E V+V   I P+E EG   +
Sbjct: 177 HHLTCPKCRHCLVETCQKI-LGDFSQADSMAST-----PTESVIVR--IDPLEPEGRVNT 225

[Close]
BLAST of Carg05293 vs. TAIR 10
Match: AT1G49220.1 (RING/U-box superfamily protein )

HSP 1 Score: 139.8 bits (351), Expect = 2.6e-33
Identity = 90/239 (37.66%), Postives = 129/239 (53.97%), Query Frame = 0 

Query: 5   ITRRFLLPTATATATATAVATPSSPPQPRPGFGSFDLNVVMVLSVLLCALVCSLGLNAIL 64
           ++R+ LL       T  A A   SP        +   NV+M+LS+L+C ++C LGL+ I+
Sbjct: 22  VSRKLLLHNPFDHNTQRAFAVAPSPLITHE--NNLSGNVMMLLSILICGIICCLGLHYII 81

Query: 65  KCALRCSTL------LASASARHGGNIAIHPKGVGRNTLKKFPTVEYSKQSNKFRGIAMD 124
           +CALR ST       + S S+  G +     KG+ +  L+ FP V YS + N      +D
Sbjct: 82  RCALRRSTRFMISEPVPSLSSTRGSS----NKGIKKKALRMFPVVSYSPEMNL---PGLD 141

Query: 125 GECVICLADFEPGDRVRILPKCYHGFHVHCIDKWLSSHTSCPKCRNCLSDTSRKV----- 184
            ECVICL+DF  G+++R+LPKC HGFHV CIDKWL  H +CPKCRNCL +T +K+     
Sbjct: 142 EECVICLSDFVSGEQLRLLPKCNHGFHVRCIDKWLQQHLTCPKCRNCLVETCQKILGDFS 201

Query: 185 --DAGCGSAVEGSSSSVGPPPPSEEVVVNVG-----------IAPVEREGLTCSYRDSS 220
             D+      E    ++ P  P+E V+V +            IAP+E EG   + R+ S
Sbjct: 202 QADSVTAEPTEIVIVTIVPLEPTEIVIVTIAPLEPTEIVIVMIAPLEPEGRVNTIREIS 251

[Close]
BLAST of Carg05293 vs. TAIR 10
Match: AT3G18773.1 (RING/U-box superfamily protein )

HSP 1 Score: 138.3 bits (347), Expect = 7.6e-33
Identity = 74/181 (40.88%), Postives = 115/181 (63.54%), Query Frame = 0 

Query: 42  NVVMVLSVLLCALVCSLGLNAILKCA-LRCSTLLAS--ASARHGGNIAIHPKGVGRNTLK 101
           NV+M+LS+LLC ++CSLGL+ I++CA +R  + + S   S       +   KG+ +  LK
Sbjct: 51  NVLMLLSILLCGIICSLGLHYIIRCAFIRSRSFMISDPISIPSTPRDSSVNKGIKKKALK 110

Query: 102 KFPTVEYSKQSNKFRGIAMDGECVICLADFEPGDRVRILPKCYHGFHVHCIDKWLSSHTS 161
             P V YS + N   G+    ECVICL+DF  G+++R+LPKC HGFH+ CIDKWL+ H +
Sbjct: 111 MLPVVNYSPEIN-LPGVG--EECVICLSDFVAGEQLRVLPKCNHGFHLRCIDKWLTQHMT 170

Query: 162 CPKCRNCLSDTSRKVDAGCGSAVEGSSSSVGPPPPSEEVVVNVGIAPVEREGLTCSYRDS 220
           CPKCR+CL DT +KV + C +A + ++++           +++ I+P+E E    ++R+S
Sbjct: 171 CPKCRHCLVDTCQKVLSDCDAADQVAATATES--------IDIRISPLEPEARVATFRES 220

[Close]
BLAST of Carg05293 vs. TAIR 10
Match: AT1G49200.1 (RING/U-box superfamily protein )

HSP 1 Score: 137.1 bits (344), Expect = 1.7e-32
Identity = 83/217 (38.25%), Postives = 122/217 (56.22%), Query Frame = 0 

Query: 5   ITRRFLLPTATATATATAVATPSSPPQPRPGFGSFDLNVVMVLSVLLCALVCSLGLNAIL 64
           ++R+ LL       T  A A   SP        +   NV+M+LSVL+C ++C LGL+ I+
Sbjct: 23  VSRKLLLHNPFDHNTQRAFAVAPSPLITHE--NNLSGNVLMLLSVLICGIICCLGLHYII 82

Query: 65  KCALR--CSTLLASASARHGGNIAIHPKGVGRNTLKKFPTVEYSKQSNKFRGIAMDGECV 124
           +CA R   S +++   A          KG+ +  L+ FP V YS + N      +  ECV
Sbjct: 83  RCAFRRTSSFMISEPIAGLSTPCGSSNKGINKKALRMFPVVSYSPEMNL---PGLGEECV 142

Query: 125 ICLADFEPGDRVRILPKCYHGFHVHCIDKWLSSHTSCPKCRNCLSDTSRKVDAGCGSAVE 184
           ICL+DF  G+++R+LPKC+HGFHV CIDKWL  H +CPKCR+CL +T +K+      A +
Sbjct: 143 ICLSDFVSGEQIRMLPKCHHGFHVRCIDKWLQQHLTCPKCRHCLVETCQKILGDFSQADQ 202

Query: 185 GSSSSVGPPPPSEEVVVNVGIAPVEREGLTCSYRDSS 220
            +++      P+  V+V   IAP+E EG     R+SS
Sbjct: 203 VAAT------PTASVIVR--IAPLEPEGRVNILRESS 226

The following BLAST results are available for this feature:
BLAST of Carg05293 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG7027130.12.6e-120100.00RING-H2 finger protein ATL78, partial [Cucurbita argyrosperma subsp. argyrosperm... [more]
XP_022963109.16.9e-11395.95RING-H2 finger protein ATL74-like [Cucurbita moschata][more]
XP_023518448.11.5e-11294.09RING-H2 finger protein ATL78-like [Cucurbita pepo subsp. pepo][more]
KAG6595117.12.0e-10795.31RING-H2 finger protein ATL78, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_038882744.11.1e-7871.68RING-H2 finger protein ATL78 [Benincasa hispida][more]
BLAST of Carg05293 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q6NQG71.5e-4149.73RING-H2 finger protein ATL78 OS=Arabidopsis thaliana OX=3702 GN=ATL78 PE=2 SV=1[more]
Q6NML09.6e-3343.24E3 ubiquitin-protein ligase ATL76 OS=Arabidopsis thaliana OX=3702 GN=ATL76 PE=1 ... [more]
P0C0343.7e-3237.66RING-H2 finger protein ATL10 OS=Arabidopsis thaliana OX=3702 GN=ATL10 PE=2 SV=1[more]
Q9LS991.1e-3140.88RING-H2 finger protein ATL77 OS=Arabidopsis thaliana OX=3702 GN=ATL77 PE=2 SV=1[more]
Q94BY62.4e-3138.25RING-H2 finger protein ATL75 OS=Arabidopsis thaliana OX=3702 GN=ATL75 PE=2 SV=1[more]
BLAST of Carg05293 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1HJ583.4e-11395.95RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111463410... [more]
A0A5A7SLG89.2e-7970.35RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3B2039.2e-7970.35RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103485255 PE=3 ... [more]
A0A0A0KF923.5e-7870.40RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G484580 PE... [more]
A0A6J1GCL73.1e-7468.33RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111452965... [more]
BLAST of Carg05293 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G49230.11.1e-4249.73RING/U-box superfamily protein [more]
AT1G49210.16.8e-3443.24RING/U-box superfamily protein [more]
AT1G49220.12.6e-3337.66RING/U-box superfamily protein [more]
AT3G18773.17.6e-3340.88RING/U-box superfamily protein [more]
AT1G49200.11.7e-3238.25RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 121..162
e-value: 3.0E-6
score: 36.8
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 120..163
e-value: 1.4E-13
score: 51.0
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 121..163
score: 11.95817
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 80..168
e-value: 4.9E-21
score: 76.2
NoneNo IPR availablePANTHERPTHR46905:SF7RING-H2 FINGER PROTEIN ATL78coord: 5..217
NoneNo IPR availableCDDcd16461RING-H2_EL5_likecoord: 120..163
e-value: 1.64387E-15
score: 65.7943
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 120..167
IPR044602RING-H2 finger protein ATL10/ATL75-79PANTHERPTHR46905RING-H2 FINGER PROTEIN ATL78coord: 5..217

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg05293-RACarg05293-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016740 transferase activity