Carg05282 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg05282
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Crossover junction endonuclease EME1B isoform X1
Location	Carg_Chr07: 3943128 .. 3948511 (-)
RNA-Seq Expression	Carg05282
Synteny	Carg05282

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCCAGCCGATTGTCCTCTCCGGAGAGGAGGACGGGGACTCTACACCTTACCCGATCCACTCCAAGAAACGTCGAACTGGATCGGATGCATTTTTGAGTATATTCTCCACTGTTATTGTGCTTGATGATGATCCGAGTACTCATAAATCAGGTCGTAACTCTCCGCCTCGAGTTATGACGTTGTAATTGTTAACTGTATGAAGGCGGCATTTGACTTTCTTGCTAAGGATTTGAATACGGACCAGAAGTTCGCTGGTTAGTGAATTTTATTCTCTTAATTGGTTCGTAGTAGTAGTTTATTATTGTTTCGGTTCGCCTCATGTGATGGATCAGTTAAATAATTTTCCTGCGCAGTAGTTTTGATCAAATAAGCTTTGTTGATGCTGGATAGGAATATTCTGAAATAGATTGGCACCGTTTGTTTGAATTGAACCCTCAGGAATCAGTGGATTGATATGCTTAGAGTTCGATAACGAGTCTGAAAGTGATTCTCAGAAGAATAAGTATGGGCACTGTGATTGGATTGATTCTGATCGTGATGATGTGAAAAACTCAGTACCGACTTCTAAATTTGACAGGGCAGGATCGGGTTCTTCTCTTGGTAGGTTCATGTTATCCTTGGTTTCCTTCCCTATTTTTGTCAGTTCCTGCATTTCTTACTGCGTGGAGAAAACAGAAGTGTGCTCATTTACGGAACTCCGCCATCCATGAAAACTGTGGACATTAATGGAAGTGTTTTGACAGGGAATGATGTTCTGCAGGAAATTTCTAGGGACACAAAGTATTCAAGTTTCGGGGGAGATGATGGTATTGATATGTATCAGGTGGATCCTTTCAAAGTAAGATCATAATATCTGTTTTCTTTCTGAAGATTGAATATAGATAGGGAGTAGTAATGGCTTCTAGATTTAGTTTTTAATGTAGCGTGGTGCATTATGAAGCATAGATACTAGATTGAGTACTACTTGCATGATAAGGTTTTTGTTTGTCAGTTTTCTCATCTCTCATCATTTGCGAAGGCGCTTGCATATTTGAAGTCATTTCTTAGCCCGCCTTCCATTGTTCCTTAGCTTGTTCTAAATCAGAATATTTACTGCAATACTACTGTAATGAAAAGTCTGTGATGCACGGCTAATACAGTGCAGTATAAGATTACACGCCCTATGTAATTTCAGAATCTTGCCCTATTTCAATGAAAGTTCTGTTTCATTTAAAAGAAAATATATTTATTACATGCTCTTTGCAATTTCAGTATCTTTGTCCTGGTTTTTCTTCATTAATTGTTGCTTGATAACTTGAAGTTGCGTGCTAGAGATTCTGTAATTTGTAACGTTTGAACTTTGTAGGGAACAGGAGAAGTACAAGAACTCAGAAAGCAATAACGGTTGAAAACGTCCACCTGTTGAGGAGGTTTGAATAACAACCATGAATGCTTAGGAGAATTAGGAAATGATTTTACCCTGCTTTCCCTAGCCAAGTTTTATACTTCAATGTTGCAGTATAAAATCTCTCATGTTCCTCACATGTTTCATCTAGAAATTGGCAAAACTGACCACTCACTTTGTCAAGGTACGTTCTAGGCAGTGTGTAGATGAAACTGAGGCAGCTGACATATTGACATGCAGTCTGGCATCCTTTCAATTCAGGTTCTGAATACTGTTCTCAAATTTCTATCAAGCATAACTTCTTGAAGTGTGAAAAGTTATTTTTCTGGATAACTTGGCGTCAATTGCAAATGTATAACTTAAATTTGTGTCGTGAAGTTTGGTCAAGTAGAAAGGATATTTCATATACTGTTCGTCGATCCCATAGACTGAACATTGTATGAAAAGAAGCCAAGAGATTCCTGTTTCGTACATCTTTAGATGTTGCATTAGTTCCATAGTTCTTTTGACATGGTACGTTAGTAAATATGGACTTAGCGATCACACCCATACACACACTCTTTCACATATTTAAATCCTTGGTCAGAATGATCTGTTTTCCTTTGCTTGTCTTGTAAAATTCAGGAAAAAGTTGACGCATCTATCCGTCAATGCAATTGGATCCATTATACCCAAAGATTGTGTTCGCAGGAATGTGATAAAGAAGTGCGTGAGGTGAGCTTTAATCGTCAATGGCCAATTCATTTCAAAATTTATTGGCATTCAGTTTCTTAATTACTCTGGCATTTACTTGATTTATATTAATGAATTTATGGCTGTAATTGAGTTACTCTCGAAGAACTCTTGTCTGTCACCAAACTGGCGGGTTAAAAGCTAACCAGAGCACTTGTCATGTATTTGATCTCAGCATCAGTCTCGTTTATGTCACAACAATATTCTAAACCATAAGAAACTATGAAACTAATATCTATCCTTCTTTGCATACCACCACTAAAATTATTATAGACCATGTGATACCACCACTAAAATTATTATATACCATGAAGTTAGTTCAATAGTTAAGGCGTTTCTACTTTTCCTAAGAGTTTATAGGTTTGAATTCTTTTATCCCAAATGTGATGATGTATTGAACTTAAAAAAGTTATATGGTACTGTCTCTGAAGTAAAACTTTATTGATTTTGTCATGTTTCTTTTTGTAATTAGGTTAGAAGCCTTGGTAGCAATTCTTATAATTAGCTTGGTTGGGATTGCAAATCAATATAAATGGAGTTGCTTTTTGTAATTAGGTTAGAAGTCTTGGTAGCAATTCCCAAGGTACAACCACGGTTTTCCGTAGCAATATGGAAGAAATATCCGACCATGAAATCTTTTTCGAAAGTGTACATGAATTCATGTAAATCTGTGAGTCTGAATCGAACAATTCACCTTATCCTCGATTGCATAAGCTAGCAGTCATGGCAGATCACATTTCTTCTTGGTCCACTCATGTAATAACAGGTGCATGAAAAGGAATTTCTGCTGAAAGACTTGACGACAGAAGGTTAGGAGAGATTTGTTCGAAGAGAGTGTACAGGATACTTATGGCTCAAAGTGGAAGCATGAGAACTGATGACGTTGAGGAGGGTGATGACTTGTTCAGATCCTAATTGTCTTAATTAATTATCATATCTAATTAGCACACCATTATTTTAATGCAATAGCTTATTGCAGAGTTTTCACCCTCGCAGACAGGTTGAAGGACTAGAGTGCCCTCATCTCTTTATCACAAATGCCACTTGGAGGCAGCGACTCGTGGAGCAAAGGGTAAATAAGTAAGTTTTCGTTGTTCAATATCCAAATCTGAATTCTCCCATCAACAAGGTAGAACTGTCCAAAATGAACTTCTTGGCTTACAATTAATTTTTCTTGCTTTTTAGTAGAGTAATTTCAACTAGGTGGGAAACCAGCATTTCACCTTGATGTTCAGTGACATAGTGTTGAACATATATATTGTGAGATCCACGATCCCACGTTGGTTGGGGAGGAGAACGAAACACCCTTTATAAGGGTGTGGAAACCTTTCCCTAGCTTAAAGCCTTGAGGGGAAGCCCGAAAGGGAAAGCCCAAAAAGGACAATATCTGCTAGCGGTGGGCTTGGGTCGTTACATATATACCTCTTTCCTTATAATTTGTCCAAAGTGCATTACAATGTTAGGTTATAGAGACGAACGTATTGACTAATCGTTCCTTGTTCTTTTAACCGTAGTTAGAAGTGAATTGATCATCTATAGAAAGATTTCTATGTAAAGTGAACATAGTTTGATATGGTATCAGAATTCAAAGGGCTAATTGCTACTTTATATTGTGTGAGTTAGTTCAAGTTTGGCATTTTTTGGTGGGAGGTTTGGTTTGGTAGTAGTAACGGTATGGTTAGGTAGAAGTTTTGATATCCTAAAAGTAGGGAGAATATTGAATTATACCAAAAAAGAAGAGACTCAAGGATGATATCAGTAATGACAACAATTTGGTGGTACTATTTTTTCTCATCCCCACCCTGCTTCCTCATATTCCAATTTTATGTGGTTCGAAATCCATCAGAAGCATTGCATTCAAACAAGTGGATTGCTCCCTTTGTAGGGTTTGTGTAATTTCCAACAGAGAGCCAAAATTTTGATGATTGTGACCCAAATCCAATTAAATTATGGTTGGGCATGGCCATTCCTCTAATCCAATTCACACTCATCCTAATTCCACCACACAAAACACCCACTAATGGGTGCGTGCTGCCACCAGCTCGCACCACTTTGTTTTTTGAATGCCTCTTTCATCCAAGGAAATCTTACCTGAATGTATAATTGATCTCTTTTAAAGTTCATGAATTATTTTTGGACAAAGGTAGAAATGGGATTGGAAGTTTTTAATACTAAAGAAGATCTAGATTAATTGATTTACCGGTTCAATCATTAGTTAATTATGTTTTTATATTGTTTTGGATTAGGTAATTGTTGCATCTTTCATAGAAGATCTGATCTCTAGACATTAGAATATAGGAAATTAAAATTCACGTTCGCAATACTCATTTTGGCACTTTCGCAAAGTGATAAGACAAGTAGAAATAGGTTAAAATATCATTTTGGTCTTTGTATCTTATACTTCATTTCACGATAAGCTTAAAATTAGACTTTACTGCCAATTTGAAGTTAAATTTTATCAAAATTAGTCAAATAATAACAATAATTTTAATGCAGTAAAAGACAATATAAACATATTTCTAAAGTATGTAGAGAAAAGCTGGTAGAGACAAAAACAAAAAACATAAGTAACCAATAGTAGGGACCAATTTAAAAGCTTAAAAGTAAATTAACTAAAATTGAATATAAAATGAAATAGCTAATATATAATGAATATATAATGAAACTCAGAAAGACTAAAATGATATTTTAACCGAACAAACATGATGTAAAATATATTTGATCAAATGTAATAGTAAAAAATTGATAAAAATTAATCACATAAGAAAGTAAGAGCACAATGCTTATATAATTAATAATTACTCTTGTCACAATTTGAGATGATGTGACATAAAACAATGACTTCTGTTCTTTATTGGTCCTCATTTCACTATCCAAATGGTAAAATGTAAGACTATCATATGAACGAATAATATCTACTGATAAAGAATACAAGGATAGTTCCTAATGTATCCTTTTAAATGACTTATACCCACATAAAGGCTGTTCTAGTAGCATATATAAGAGAGCAGACATACTTATGAAACATATCCAAAGTTTGTAATGATGGAAAGAAGCAAATCATTTTGTGGGTCTTACTCAAAAGCCTATGGAGATGTGAGGTTTGGATTTGAGGACAAAACCAAATCATACAGCTTCAATGGGACAATGAACAAAGAAGATGAGGGCAATCCAGAGCTCAAGAGAAAGCAAAGAGTAGCTACTTACAATATGTATTCCCTGGAAGGTAAGCTGAAGAAGTCCTTGAGAAAAAGCTTCAAGTAG

mRNA sequence

ATGTCCCAGCCGATTGTCCTCTCCGGAGAGGAGGACGGGGACTCTACACCTTACCCGATCCACTCCAAGAAACGTCGAACTGGATCGGATGCATTTTTGAGTATATTCTCCACTGCGGCATTTGACTTTCTTGCTAAGGATTTGAATACGGACCAGAAGTTCGCTGGAATCAGTGGATTGATATGCTTAGAGTTCGATAACGAGTCTGAAAGTGATTCTCAGAAGAATAAGTATGGGCACTGTGATTGGATTGATTCTGATCGTGATGATGTGAAAAACTCATTCCTGCATTTCTTACTGCGTGGAGAAAACAGAAGTGTGCTCATTTACGGAACTCCGCCATCCATGAAAACTGTGGACATTAATGGAAGTGTTTTGACAGGGAATGATGTTCTGCAGGAAATTTCTAGGGACACAAAGTATTCAAGTTTCGGGGGAGATGATGGTATTGATATGTATCAGGTGGATCCTTTCAAAAAATTGGCAAAACTGACCACTCACTTTGTCAAGGTACGTTCTAGGCAGTGTGTAGATGAAACTGAGGCAGCTGACATATTGACATGCAGTCTGGCATCCTTTCAATTCAGGAAAAAGTTGACGCATCTATCCGTCAATGCAATTGGATCCATTATACCCAAAGATTGTGTTCGCAGGAATGTGATAAAGAAGTGCGTGAGGTTAGAAGTCTTGGTAGCAATTCCCAAGGTACAACCACGGTTTTCCGTAGCAATATGGAAGAAATATCCGACCATGAAATCTTTTTCGAAAGTGTACATGAATTCATGTAAATCTGTGCATGAAAAGGAATTTCTGCTGAAAGACTTGACGACAGAAGCCTATGGAGATGTGAGGTTTGGATTTGAGGACAAAACCAAATCATACAGCTTCAATGGGACAATGAACAAAGAAGATGAGGGCAATCCAGAGCTCAAGAGAAAGCAAAGAGTAGCTACTTACAATATGTATTCCCTGGAAGGTAAGCTGAAGAAGTCCTTGAGAAAAAGCTTCAAGTAG

Coding sequence (CDS)

ATGTCCCAGCCGATTGTCCTCTCCGGAGAGGAGGACGGGGACTCTACACCTTACCCGATCCACTCCAAGAAACGTCGAACTGGATCGGATGCATTTTTGAGTATATTCTCCACTGCGGCATTTGACTTTCTTGCTAAGGATTTGAATACGGACCAGAAGTTCGCTGGAATCAGTGGATTGATATGCTTAGAGTTCGATAACGAGTCTGAAAGTGATTCTCAGAAGAATAAGTATGGGCACTGTGATTGGATTGATTCTGATCGTGATGATGTGAAAAACTCATTCCTGCATTTCTTACTGCGTGGAGAAAACAGAAGTGTGCTCATTTACGGAACTCCGCCATCCATGAAAACTGTGGACATTAATGGAAGTGTTTTGACAGGGAATGATGTTCTGCAGGAAATTTCTAGGGACACAAAGTATTCAAGTTTCGGGGGAGATGATGGTATTGATATGTATCAGGTGGATCCTTTCAAAAAATTGGCAAAACTGACCACTCACTTTGTCAAGGTACGTTCTAGGCAGTGTGTAGATGAAACTGAGGCAGCTGACATATTGACATGCAGTCTGGCATCCTTTCAATTCAGGAAAAAGTTGACGCATCTATCCGTCAATGCAATTGGATCCATTATACCCAAAGATTGTGTTCGCAGGAATGTGATAAAGAAGTGCGTGAGGTTAGAAGTCTTGGTAGCAATTCCCAAGGTACAACCACGGTTTTCCGTAGCAATATGGAAGAAATATCCGACCATGAAATCTTTTTCGAAAGTGTACATGAATTCATGTAAATCTGTGCATGAAAAGGAATTTCTGCTGAAAGACTTGACGACAGAAGCCTATGGAGATGTGAGGTTTGGATTTGAGGACAAAACCAAATCATACAGCTTCAATGGGACAATGAACAAAGAAGATGAGGGCAATCCAGAGCTCAAGAGAAAGCAAAGAGTAGCTACTTACAATATGTATTCCCTGGAAGGTAAGCTGAAGAAGTCCTTGAGAAAAAGCTTCAAGTAG

Protein sequence

MSQPIVLSGEEDGDSTPYPIHSKKRRTGSDAFLSIFSTAAFDFLAKDLNTDQKFAGISGLICLEFDNESESDSQKNKYGHCDWIDSDRDDVKNSFLHFLLRGENRSVLIYGTPPSMKTVDINGSVLTGNDVLQEISRDTKYSSFGGDDGIDMYQVDPFKKLAKLTTHFVKVRSRQCVDETEAADILTCSLASFQFRKKLTHLSVNAIGSIIPKDCVRRNVIKKCVRLEVLVAIPKVQPRFSVAIWKKYPTMKSFSKVYMNSCKSVHEKEFLLKDLTTEAYGDVRFGFEDKTKSYSFNGTMNKEDEGNPELKRKQRVATYNMYSLEGKLKKSLRKSFK

Homology

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BLAST of Carg05282 vs. NCBI nr
Match: KAG7027119.1 (Crossover junction endonuclease EME1B, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 676.0 bits (1743), Expect = 1.7e-190
Identity = 337/337 (100.00%), Postives = 337/337 (100.00%), Query Frame = 0


Query: 1   MSQPIVLSGEEDGDSTPYPIHSKKRRTGSDAFLSIFSTAAFDFLAKDLNTDQKFAGISGL 60
           MSQPIVLSGEEDGDSTPYPIHSKKRRTGSDAFLSIFSTAAFDFLAKDLNTDQKFAGISGL
Sbjct: 1   MSQPIVLSGEEDGDSTPYPIHSKKRRTGSDAFLSIFSTAAFDFLAKDLNTDQKFAGISGL 60

Query: 61  ICLEFDNESESDSQKNKYGHCDWIDSDRDDVKNSFLHFLLRGENRSVLIYGTPPSMKTVD 120
           ICLEFDNESESDSQKNKYGHCDWIDSDRDDVKNSFLHFLLRGENRSVLIYGTPPSMKTVD
Sbjct: 61  ICLEFDNESESDSQKNKYGHCDWIDSDRDDVKNSFLHFLLRGENRSVLIYGTPPSMKTVD 120

Query: 121 INGSVLTGNDVLQEISRDTKYSSFGGDDGIDMYQVDPFKKLAKLTTHFVKVRSRQCVDET 180
           INGSVLTGNDVLQEISRDTKYSSFGGDDGIDMYQVDPFKKLAKLTTHFVKVRSRQCVDET
Sbjct: 121 INGSVLTGNDVLQEISRDTKYSSFGGDDGIDMYQVDPFKKLAKLTTHFVKVRSRQCVDET 180

Query: 181 EAADILTCSLASFQFRKKLTHLSVNAIGSIIPKDCVRRNVIKKCVRLEVLVAIPKVQPRF 240
           EAADILTCSLASFQFRKKLTHLSVNAIGSIIPKDCVRRNVIKKCVRLEVLVAIPKVQPRF
Sbjct: 181 EAADILTCSLASFQFRKKLTHLSVNAIGSIIPKDCVRRNVIKKCVRLEVLVAIPKVQPRF 240

Query: 241 SVAIWKKYPTMKSFSKVYMNSCKSVHEKEFLLKDLTTEAYGDVRFGFEDKTKSYSFNGTM 300
           SVAIWKKYPTMKSFSKVYMNSCKSVHEKEFLLKDLTTEAYGDVRFGFEDKTKSYSFNGTM
Sbjct: 241 SVAIWKKYPTMKSFSKVYMNSCKSVHEKEFLLKDLTTEAYGDVRFGFEDKTKSYSFNGTM 300

Query: 301 NKEDEGNPELKRKQRVATYNMYSLEGKLKKSLRKSFK 338
           NKEDEGNPELKRKQRVATYNMYSLEGKLKKSLRKSFK
Sbjct: 301 NKEDEGNPELKRKQRVATYNMYSLEGKLKKSLRKSFK 337

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BLAST of Carg05282 vs. NCBI nr
Match: KAG6595104.1 (Crossover junction endonuclease EME1B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 479.2 bits (1232), Expect = 3.0e-131
Identity = 250/287 (87.11%), Postives = 251/287 (87.46%), Query Frame = 0


Query: 1   MSQPIVLSGEEDGDSTPYPIHSKKRRTGSDAFLSIFST--AAFDFLAKDLNTDQKFAGIS 60
           MSQPIVLSGEEDGDSTPYPIHSKKRRTGSDAFLSIFST  AAFDFLAKDLNTDQKFAGIS
Sbjct: 1   MSQPIVLSGEEDGDSTPYPIHSKKRRTGSDAFLSIFSTVIAAFDFLAKDLNTDQKFAGIS 60

Query: 61  GLICLEFDNESESDSQKNKYGHCDWIDSDRDDVKNSFLHFLLRGENRSVLIYGTPPSMKT 120
           GLICLEFDNESESDSQKNKYGHCDWIDSDRDDVKNS                   P+ K 
Sbjct: 61  GLICLEFDNESESDSQKNKYGHCDWIDSDRDDVKNSV------------------PTSKF 120

Query: 121 VDINGSVLTGNDVLQEISRDTKYSSFGGDDGIDMYQVDPFKKLAKLTTHFVKVRSRQCVD 180
                    GNDVLQEISRDTKYSSFGGDDGIDMYQVDPFKKLAKLTTHFVKVRSRQCVD
Sbjct: 121 DRAGSGSSLGNDVLQEISRDTKYSSFGGDDGIDMYQVDPFKKLAKLTTHFVKVRSRQCVD 180

Query: 181 ETEAADILTCSLASFQFRKKLTHLSVNAIGSIIPKDCVRRNVIKKCVRLEVLVAIPKVQP 240
           ETEAADILTCSLASFQFRKKLTHLSVNAIGSIIPKDCVRRNVIKKCVRLEVLVAIPKVQP
Sbjct: 181 ETEAADILTCSLASFQFRKKLTHLSVNAIGSIIPKDCVRRNVIKKCVRLEVLVAIPKVQP 240

Query: 241 RFSVAIWKKYPTMKSFSKVYMNSCKSVHEKEFLLKDLTTEAYGDVRF 286
           RFSVAIWKKYPTMKSFSKVYMNSCKSVHEKEFLLKDLTTE  G  RF
Sbjct: 241 RFSVAIWKKYPTMKSFSKVYMNSCKSVHEKEFLLKDLTTE--GQERF 267

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BLAST of Carg05282 vs. NCBI nr
Match: TYK12984.1 (crossover junction endonuclease EME1B isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 223.8 bits (569), Expect = 2.3e-54
Identity = 194/552 (35.14%), Postives = 219/552 (39.67%), Query Frame = 0


Query: 1   MSQPIVLSGEEDGDSTPYPIHSKKRRTGSDAFLSIFST---------------------- 60
           MSQPIVLS EED  STP PI SKKRRT SD   SI ST                      
Sbjct: 1   MSQPIVLSDEEDRYSTPIPIPSKKRRTESDLLPSIKSTVLVLDDDPTPQKSNHNSAASFV 60

Query: 61  --------------------AAFDFLAKDLNTDQKFAGISGLICLEFDNESESDSQKNKY 120
                               A  +  A+DLN+DQKFAGISGLICLE DNESESDSQK K 
Sbjct: 61  PETPLSPQPSSDVAIVKCTKAGLNLHARDLNSDQKFAGISGLICLESDNESESDSQKRKC 120

Query: 121 GHCDWIDSDRDDVKN--SFLHFLLRGENRSVLIYGTPPSMKTVDINGSVLTGNDVLQEIS 180
              +WI SD  ++KN  + L F   G   S                     GND LQEIS
Sbjct: 121 EQSEWIGSDCYEMKNIGASLPFDSAGSGSS--------------------HGNDDLQEIS 180

Query: 181 RDTKYSSFGGDDGIDMYQVDPF-------------------------------------- 240
            DTK+S+FG D   D+YQV  +                                      
Sbjct: 181 GDTKHSAFGRDG--DIYQVHDYPEKDNVGMEQNDNSIKTTGRKANAEKLEKLQKAAQKAE 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 AAEMKKMQKEKQKWEKGKLALKSIVAEIDAKVVELGSIGGHLLTRFAEKGITFRIKSNPI 300

Query: 301 ------------------------------------------------------------ 315
                                                                       
Sbjct: 301 ERSILWTMNVPEHISASSKGLEISYVLLVYEAENFCELLSKESFMDHVARVQSRYPSHTI 360

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BLAST of Carg05282 vs. NCBI nr
Match: XP_038880967.1 (crossover junction endonuclease EME1B-like [Benincasa hispida])

HSP 1 Score: 218.0 bits (554), Expect = 1.2e-52
Identity = 197/587 (33.56%), Postives = 221/587 (37.65%), Query Frame = 0


Query: 1   MSQPIVLSGEEDGDSTPYPIHSKKRRTGSDAFLSIFST---------------------- 60
           MSQPIVLS EED +STP PI SKKRRT SD F  I ST                      
Sbjct: 1   MSQPIVLSDEEDRNSTPLPIRSKKRRTESDVFPGIKSTVLVLDDDPTPQKSSRNSAVSFV 60

Query: 61  --------------------AAFDFLAKDLNTDQKFAGISGLICLEFDNESESDSQKNKY 120
                               A  +  A+ L +DQKFAGISGLICLE DNESESDSQK K 
Sbjct: 61  PETPLSPPPSSDVAIVKCTKARLNLHARVLTSDQKFAGISGLICLESDNESESDSQKEKC 120

Query: 121 GHCDWIDSDRDDVKNSFLHFLLRGENRSVLIYGTPPSMKTVDINGSVLTGNDVLQEISRD 180
           G  +WI SD  ++KN        G        G+ PS+           GND LQEIS D
Sbjct: 121 GPSEWIGSDCYEMKNI-------GSTFQFDSAGSGPSL-----------GNDDLQEISGD 180

Query: 181 TKYSSFGGDDGIDMYQVDPF---------------------------------------- 240
           TK+S+FG DD  D+YQV  +                                        
Sbjct: 181 TKHSTFGQDD--DIYQVHDYPEKESVGMEQNDNSIKATGRKANAEKNKSIDKVMRKKATK 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 DDKIRLMEEKKKKKELEKLQKAAQKAEAAEMKKMQKEKQKWEKGKLALKSIVAEIDAKVV 300

Query: 301 ------------------------------------------------------------ 315
                                                                       
Sbjct: 301 ELGSVGGHLLTRFAEKGITFRIKSNPIERSILWTMNVPEHISESSKGLEISYILLVYEAE 360

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BLAST of Carg05282 vs. NCBI nr
Match: XP_022977604.1 (crossover junction endonuclease EME1B isoform X3 [Cucurbita maxima])

HSP 1 Score: 216.5 bits (550), Expect = 3.6e-52
Identity = 196/578 (33.91%), Postives = 224/578 (38.75%), Query Frame = 0


Query: 1   MSQPIVLSGEEDGDSTPYPIHSKKRRTGSDAFLSIFST------------------AAF- 60
           MS+PIVLS EED  STP PI SKKRRT S AF SI ST                  A+F 
Sbjct: 1   MSRPIVLSDEEDRYSTPLPIPSKKRRTESHAFPSIKSTVLVLDDDPTPQKSGSNSAASFV 60

Query: 61  -----------------------DFLAKDLNTDQKFAGISGLICLEFDNESESDSQKNKY 120
                                  D  A+DLN+D KFAGISGLICLE DNESESDS K   
Sbjct: 61  PETPLSPPPSSDVVIVKCTKVPLDLPARDLNSDHKFAGISGLICLESDNESESDSLKKMC 120

Query: 121 GHCDWIDSDRDDVKNSFLHFLLRGENRSVLIYGTPPSMKTVDINGSVLTGNDVLQEISRD 180
           G  +WI SD DD+KN        G        G+ PS+           GND L EIS D
Sbjct: 121 GPSEWIGSDCDDMKN-------LGSTSKFDSAGSGPSI-----------GNDDLHEISGD 180

Query: 181 TKYSSFGGDD-------------------------------------------------- 240
           TK+S+ G DD                                                  
Sbjct: 181 TKHSTVGRDDYIYQENVGMEQNDNTIKPTGRKSDAEKNKNVDKAMRKKATKDDKIRLMEE 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 KKKKKELEKLQKAAQKAEAAEMRKMQKEKQKWEKGKLALKSIVAEIDAKVVELGSVGGHL 300

Query: 301 -------------------------------------GID------MYQVDPF------- 315
                                                G++      MY+ + F       
Sbjct: 301 LTRFAEKGITFRIKSNPIERSIVWTMNAPEHISESFKGLEIAYVLLMYEAENFCELLSKE 360

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BLAST of Carg05282 vs. ExPASy Swiss-Prot
Match: C5H8J1 (Crossover junction endonuclease EME1B OS=Arabidopsis thaliana OX=3702 GN=EME1B PE=1 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 7.6e-37
Identity = 89/156 (57.05%), Postives = 110/156 (70.51%), Query Frame = 0


Query: 161 LAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFRKKLTHLSVNAIGSIIPKDCVR 220
           LAKLTTH+VKV SR CVDE E A+    LT SLAS QFRKKLT LSV+A G+++ KD V 
Sbjct: 384 LAKLTTHYVKVHSRHCVDEAEVAEHIVGLTSSLASCQFRKKLTMLSVSANGALVSKDSVD 443

Query: 221 RNVIKKCVRLEVLVAIPKVQPRFSVAIWKKYPTMKSFSKVYMNSCKSVHEKEFLLKDLTT 280
           +++IKK   L+ LVAIPKVQPR+++A+WKKYP+MKS  KVYM+  KSVHEKEFLLKDL  
Sbjct: 444 KHLIKKSPWLKALVAIPKVQPRYALAVWKKYPSMKSLLKVYMDRNKSVHEKEFLLKDLKV 503

Query: 281 EAY--GDVRFGFEDKTKSY----SFNGTMNKEDEGN 308
           E    GD+R G     + Y    S +G +  +D  N
Sbjct: 504 EGLVGGDIRLGEICSKRIYRVLMSHDGAIKTDDVEN 539

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BLAST of Carg05282 vs. ExPASy Swiss-Prot
Match: Q84M98 (Crossover junction endonuclease EME1A OS=Arabidopsis thaliana OX=3702 GN=EME1A PE=1 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 3.4e-29
Identity = 77/156 (49.36%), Postives = 103/156 (66.03%), Query Frame = 0


Query: 161 LAKLTTHFVKVRSRQCVDETEAAD---ILTCSLASFQFRKKLTHLSVNAIGSIIPKDCVR 220
           +AKL+TH++ V SR CVDE E AD    LT SLA  Q RKKLT LSV A G+++ K+   
Sbjct: 378 IAKLSTHYIGVHSRHCVDEAEVADHVVRLTSSLAHCQVRKKLTRLSVYADGTLMSKNAAD 437

Query: 221 RNVIKKCVRLEVLVAIPKVQPRFSVAIWKKYPTMKSFSKVYMNSCKSVHEKEFLLKDLTT 280
           +++I++ + L+VLVAIPKVQPR+++A+ KKYP++KS  KVYM+   SVHEKEFLLKDL  
Sbjct: 438 KHLIRESIWLKVLVAIPKVQPRYAIAVSKKYPSLKSLLKVYMDPNISVHEKEFLLKDLKV 497

Query: 281 E--AYGDVRFGFEDKTKSY----SFNGTMNKEDEGN 308
           E     D   G     + Y    S +GT+  +D  N
Sbjct: 498 ENLVGRDTSVGEACSKRIYRVLMSLDGTIKTDDVEN 533

[Close]

BLAST of Carg05282 vs. ExPASy Swiss-Prot
Match: Q0J9J6 (Crossover junction endonuclease EME1 OS=Oryza sativa subsp. japonica OX=39947 GN=EME1 PE=2 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.7e-25
Identity = 67/121 (55.37%), Postives = 82/121 (67.77%), Query Frame = 0


Query: 161 LAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFRKKLTHLSVNAIGSIIPKDCVR 220
           L KL TH+ +VRSR C DE E  +    LT SLA+ +FRK LT LSV+A GS I K CV 
Sbjct: 492 LCKLATHYARVRSRHCTDEAEVTEHIVGLTYSLANCKFRKPLTWLSVHANGSNISKGCVD 551

Query: 221 RNVIKKCVRLEVLVAIPKVQPRFSVAIWKKYPTMKSFSKVYMNSCKSVHEKEFLLKDLTT 279
           ++ IKK   L+ LVAIP V P  ++AI KKYP+M+S   VYM+  KSVHEKE LL+DL  
Sbjct: 552 KDRIKKSAWLKSLVAIPGVSPGQAIAIEKKYPSMRSLLNVYMDDSKSVHEKEHLLEDLRL 611

[Close]

BLAST of Carg05282 vs. ExPASy TrEMBL
Match: A0A5D3CPM4 (Crossover junction endonuclease EME1B isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G005810 PE=3 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 1.1e-54
Identity = 194/552 (35.14%), Postives = 219/552 (39.67%), Query Frame = 0


Query: 1   MSQPIVLSGEEDGDSTPYPIHSKKRRTGSDAFLSIFST---------------------- 60
           MSQPIVLS EED  STP PI SKKRRT SD   SI ST                      
Sbjct: 1   MSQPIVLSDEEDRYSTPIPIPSKKRRTESDLLPSIKSTVLVLDDDPTPQKSNHNSAASFV 60

Query: 61  --------------------AAFDFLAKDLNTDQKFAGISGLICLEFDNESESDSQKNKY 120
                               A  +  A+DLN+DQKFAGISGLICLE DNESESDSQK K 
Sbjct: 61  PETPLSPQPSSDVAIVKCTKAGLNLHARDLNSDQKFAGISGLICLESDNESESDSQKRKC 120

Query: 121 GHCDWIDSDRDDVKN--SFLHFLLRGENRSVLIYGTPPSMKTVDINGSVLTGNDVLQEIS 180
              +WI SD  ++KN  + L F   G   S                     GND LQEIS
Sbjct: 121 EQSEWIGSDCYEMKNIGASLPFDSAGSGSS--------------------HGNDDLQEIS 180

Query: 181 RDTKYSSFGGDDGIDMYQVDPF-------------------------------------- 240
            DTK+S+FG D   D+YQV  +                                      
Sbjct: 181 GDTKHSAFGRDG--DIYQVHDYPEKDNVGMEQNDNSIKTTGRKANAEKLEKLQKAAQKAE 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 AAEMKKMQKEKQKWEKGKLALKSIVAEIDAKVVELGSIGGHLLTRFAEKGITFRIKSNPI 300

Query: 301 ------------------------------------------------------------ 315
                                                                       
Sbjct: 301 ERSILWTMNVPEHISASSKGLEISYVLLVYEAENFCELLSKESFMDHVARVQSRYPSHTI 360

[Close]

BLAST of Carg05282 vs. ExPASy TrEMBL
Match: A0A6J1IRV2 (crossover junction endonuclease EME1B isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111477883 PE=3 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 1.7e-52
Identity = 196/578 (33.91%), Postives = 224/578 (38.75%), Query Frame = 0


Query: 1   MSQPIVLSGEEDGDSTPYPIHSKKRRTGSDAFLSIFST------------------AAF- 60
           MS+PIVLS EED  STP PI SKKRRT S AF SI ST                  A+F 
Sbjct: 1   MSRPIVLSDEEDRYSTPLPIPSKKRRTESHAFPSIKSTVLVLDDDPTPQKSGSNSAASFV 60

Query: 61  -----------------------DFLAKDLNTDQKFAGISGLICLEFDNESESDSQKNKY 120
                                  D  A+DLN+D KFAGISGLICLE DNESESDS K   
Sbjct: 61  PETPLSPPPSSDVVIVKCTKVPLDLPARDLNSDHKFAGISGLICLESDNESESDSLKKMC 120

Query: 121 GHCDWIDSDRDDVKNSFLHFLLRGENRSVLIYGTPPSMKTVDINGSVLTGNDVLQEISRD 180
           G  +WI SD DD+KN        G        G+ PS+           GND L EIS D
Sbjct: 121 GPSEWIGSDCDDMKN-------LGSTSKFDSAGSGPSI-----------GNDDLHEISGD 180

Query: 181 TKYSSFGGDD-------------------------------------------------- 240
           TK+S+ G DD                                                  
Sbjct: 181 TKHSTVGRDDYIYQENVGMEQNDNTIKPTGRKSDAEKNKNVDKAMRKKATKDDKIRLMEE 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 KKKKKELEKLQKAAQKAEAAEMRKMQKEKQKWEKGKLALKSIVAEIDAKVVELGSVGGHL 300

Query: 301 -------------------------------------GID------MYQVDPF------- 315
                                                G++      MY+ + F       
Sbjct: 301 LTRFAEKGITFRIKSNPIERSIVWTMNAPEHISESFKGLEIAYVLLMYEAENFCELLSKE 360

[Close]

BLAST of Carg05282 vs. ExPASy TrEMBL
Match: A0A6J1IQH9 (crossover junction endonuclease EME1B isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111477883 PE=3 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 5.1e-52
Identity = 196/582 (33.68%), Postives = 224/582 (38.49%), Query Frame = 0


Query: 1   MSQPIVLSGEEDGDSTPYPIHSKKRRTGSDAFLSIFST------------------AAF- 60
           MS+PIVLS EED  STP PI SKKRRT S AF SI ST                  A+F 
Sbjct: 1   MSRPIVLSDEEDRYSTPLPIPSKKRRTESHAFPSIKSTVLVLDDDPTPQKSGSNSAASFV 60

Query: 61  -----------------------DFLAKDLNTDQKFAGISGLICLEFDNESESDSQKNKY 120
                                  D  A+DLN+D KFAGISGLICLE DNESESDS K   
Sbjct: 61  PETPLSPPPSSDVVIVKCTKVPLDLPARDLNSDHKFAGISGLICLESDNESESDSLKKMC 120

Query: 121 GHCDWIDSDRDDVKNSFLHFLLRGENRSVLIYGTPPSMKTVDINGSVLTGNDVLQEISRD 180
           G  +WI SD DD+KN        G        G+ PS+           GND L EIS D
Sbjct: 121 GPSEWIGSDCDDMKN-------LGSTSKFDSAGSGPSI-----------GNDDLHEISGD 180

Query: 181 TKYSSFGGDD-------------------------------------------------- 240
           TK+S+ G DD                                                  
Sbjct: 181 TKHSTVGRDDYIYQVHDYPEKENVGMEQNDNTIKPTGRKSDAEKNKNVDKAMRKKATKDD 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 KIRLMEEKKKKKELEKLQKAAQKAEAAEMRKMQKEKQKWEKGKLALKSIVAEIDAKVVEL 300

Query: 301 -----------------------------------------GID------MYQVDPF--- 315
                                                    G++      MY+ + F   
Sbjct: 301 GSVGGHLLTRFAEKGITFRIKSNPIERSIVWTMNAPEHISEGLEIAYVLLMYEAENFCEL 360

[Close]

BLAST of Carg05282 vs. ExPASy TrEMBL
Match: A0A6J1IIZ2 (crossover junction endonuclease EME1B isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111477883 PE=3 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 1.1e-51
Identity = 196/585 (33.50%), Postives = 224/585 (38.29%), Query Frame = 0


Query: 1   MSQPIVLSGEEDGDSTPYPIHSKKRRTGSDAFLSIFST------------------AAF- 60
           MS+PIVLS EED  STP PI SKKRRT S AF SI ST                  A+F 
Sbjct: 1   MSRPIVLSDEEDRYSTPLPIPSKKRRTESHAFPSIKSTVLVLDDDPTPQKSGSNSAASFV 60

Query: 61  -----------------------DFLAKDLNTDQKFAGISGLICLEFDNESESDSQKNKY 120
                                  D  A+DLN+D KFAGISGLICLE DNESESDS K   
Sbjct: 61  PETPLSPPPSSDVVIVKCTKVPLDLPARDLNSDHKFAGISGLICLESDNESESDSLKKMC 120

Query: 121 GHCDWIDSDRDDVKNSFLHFLLRGENRSVLIYGTPPSMKTVDINGSVLTGNDVLQEISRD 180
           G  +WI SD DD+KN        G        G+ PS+           GND L EIS D
Sbjct: 121 GPSEWIGSDCDDMKN-------LGSTSKFDSAGSGPSI-----------GNDDLHEISGD 180

Query: 181 TKYSSFGGDD-------------------------------------------------- 240
           TK+S+ G DD                                                  
Sbjct: 181 TKHSTVGRDDYIYQVHDYPEKENVGMEQNDNTIKPTGRKSDAEKNKNVDKAMRKKATKDD 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 KIRLMEEKKKKKELEKLQKAAQKAEAAEMRKMQKEKQKWEKGKLALKSIVAEIDAKVVEL 300

Query: 301 --------------------------------------------GID------MYQVDPF 315
                                                       G++      MY+ + F
Sbjct: 301 GSVGGHLLTRFAEKGITFRIKSNPIERSIVWTMNAPEHISESFKGLEIAYVLLMYEAENF 360

[Close]

BLAST of Carg05282 vs. ExPASy TrEMBL
Match: A0A1S3AZW5 (crossover junction endonuclease EME1B isoform X2 OS=Cucumis melo OX=3656 GN=LOC103484673 PE=3 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 1.5e-51
Identity = 186/547 (34.00%), Postives = 206/547 (37.66%), Query Frame = 0


Query: 1   MSQPIVLSGEEDGDSTPYPIHSKKRRTGSDAFLSIFST---------------------- 60
           MSQPIVLS EED  STP PI SKKRRT SD   SI ST                      
Sbjct: 1   MSQPIVLSDEEDRYSTPIPIPSKKRRTESDLLPSIKSTVLVLDDDPTPQKSNHNSAASFV 60

Query: 61  --------------------AAFDFLAKDLNTDQKFAGISGLICLEFDNESESDSQKNKY 120
                               A  +  A+DLN+DQKFAGISGLICLE DNESESDSQK K 
Sbjct: 61  PETPLSPQLSSDVAIVKCTKAGLNLHARDLNSDQKFAGISGLICLESDNESESDSQKRKC 120

Query: 121 GHCDWIDSDRDDVKN--SFLHFLLRGENRSVLIYGTPPSMKTVDINGSVLTGNDVLQEIS 180
              +WI SD  ++KN  + L F   G   S                     GND LQEIS
Sbjct: 121 EQSEWIGSDCYEMKNIGASLPFDSAGSGSS--------------------HGNDDLQEIS 180

Query: 181 RDTKYSSFGGDDGIDMYQVDPF-------------------------------------- 240
            DTK+S+FG D   D+YQV  +                                      
Sbjct: 181 GDTKHSAFGRDG--DIYQVHDYPEKDNVGMEQNDNSIKTTGRKANAEKVMRKKATKDDKI 240

Query: 241 ------------------------------------------------------------ 285
                                                                       
Sbjct: 241 RLMEEKKKKKELEKLQKAAQKAEAAEMKKMQKEKQKWEKGKLALKSIVAEIDAKVVELGS 300

[Close]

BLAST of Carg05282 vs. TAIR 10
Match: AT2G22140.1 (essential meiotic endonuclease 1B )

HSP 1 Score: 156.0 bits (393), Expect = 5.4e-38
Identity = 89/156 (57.05%), Postives = 110/156 (70.51%), Query Frame = 0


Query: 161 LAKLTTHFVKVRSRQCVDETEAADI---LTCSLASFQFRKKLTHLSVNAIGSIIPKDCVR 220
           LAKLTTH+VKV SR CVDE E A+    LT SLAS QFRKKLT LSV+A G+++ KD V 
Sbjct: 384 LAKLTTHYVKVHSRHCVDEAEVAEHIVGLTSSLASCQFRKKLTMLSVSANGALVSKDSVD 443

Query: 221 RNVIKKCVRLEVLVAIPKVQPRFSVAIWKKYPTMKSFSKVYMNSCKSVHEKEFLLKDLTT 280
           +++IKK   L+ LVAIPKVQPR+++A+WKKYP+MKS  KVYM+  KSVHEKEFLLKDL  
Sbjct: 444 KHLIKKSPWLKALVAIPKVQPRYALAVWKKYPSMKSLLKVYMDRNKSVHEKEFLLKDLKV 503

Query: 281 EAY--GDVRFGFEDKTKSY----SFNGTMNKEDEGN 308
           E    GD+R G     + Y    S +G +  +D  N
Sbjct: 504 EGLVGGDIRLGEICSKRIYRVLMSHDGAIKTDDVEN 539

[Close]

BLAST of Carg05282 vs. TAIR 10
Match: AT2G21800.1 (essential meiotic endonuclease 1A )

HSP 1 Score: 130.6 bits (327), Expect = 2.4e-30
Identity = 77/156 (49.36%), Postives = 103/156 (66.03%), Query Frame = 0


Query: 161 LAKLTTHFVKVRSRQCVDETEAAD---ILTCSLASFQFRKKLTHLSVNAIGSIIPKDCVR 220
           +AKL+TH++ V SR CVDE E AD    LT SLA  Q RKKLT LSV A G+++ K+   
Sbjct: 378 IAKLSTHYIGVHSRHCVDEAEVADHVVRLTSSLAHCQVRKKLTRLSVYADGTLMSKNAAD 437

Query: 221 RNVIKKCVRLEVLVAIPKVQPRFSVAIWKKYPTMKSFSKVYMNSCKSVHEKEFLLKDLTT 280
           +++I++ + L+VLVAIPKVQPR+++A+ KKYP++KS  KVYM+   SVHEKEFLLKDL  
Sbjct: 438 KHLIRESIWLKVLVAIPKVQPRYAIAVSKKYPSLKSLLKVYMDPNISVHEKEFLLKDLKV 497

Query: 281 E--AYGDVRFGFEDKTKSY----SFNGTMNKEDEGN 308
           E     D   G     + Y    S +GT+  +D  N
Sbjct: 498 ENLVGRDTSVGEACSKRIYRVLMSLDGTIKTDDVEN 533

[Close]

BLAST of Carg05282 vs. TAIR 10
Match: AT2G21800.2 (essential meiotic endonuclease 1A )

HSP 1 Score: 130.6 bits (327), Expect = 2.4e-30
Identity = 77/156 (49.36%), Postives = 103/156 (66.03%), Query Frame = 0


Query: 161 LAKLTTHFVKVRSRQCVDETEAAD---ILTCSLASFQFRKKLTHLSVNAIGSIIPKDCVR 220
           +AKL+TH++ V SR CVDE E AD    LT SLA  Q RKKLT LSV A G+++ K+   
Sbjct: 381 IAKLSTHYIGVHSRHCVDEAEVADHVVRLTSSLAHCQVRKKLTRLSVYADGTLMSKNAAD 440

Query: 221 RNVIKKCVRLEVLVAIPKVQPRFSVAIWKKYPTMKSFSKVYMNSCKSVHEKEFLLKDLTT 280
           +++I++ + L+VLVAIPKVQPR+++A+ KKYP++KS  KVYM+   SVHEKEFLLKDL  
Sbjct: 441 KHLIRESIWLKVLVAIPKVQPRYAIAVSKKYPSLKSLLKVYMDPNISVHEKEFLLKDLKV 500

Query: 281 E--AYGDVRFGFEDKTKSY----SFNGTMNKEDEGN 308
           E     D   G     + Y    S +GT+  +D  N
Sbjct: 501 ENLVGRDTSVGEACSKRIYRVLMSLDGTIKTDDVEN 536

[Close]

BLAST of Carg05282 vs. TAIR 10
Match: AT4G09890.1 (Protein of unknown function (DUF3511) )

HSP 1 Score: 50.1 bits (118), Expect = 4.2e-06
Identity = 26/62 (41.94%), Postives = 43/62 (69.35%), Query Frame = 0


Query: 277 TEAYGDVRFGF-EDKTKSYSFNGTMNKEDEGNPELKRKQRVATYNMYSLEGKLKKSLRKS 336
           + ++    FG+ ++++ SY+FNG     D   PE+KRK+RVA+YN+++ E KLK +L+ S
Sbjct: 12  SSSFSTDEFGYDQNRSNSYNFNGPCINTD---PEMKRKKRVASYNLFATEEKLKSTLKNS 70

Query: 337 FK 338
           FK
Sbjct: 72  FK 70

[Close]

BLAST of Carg05282 vs. TAIR 10
Match: AT3G05725.1 (Protein of unknown function (DUF3511) )

HSP 1 Score: 43.9 bits (102), Expect = 3.0e-04
Identity = 23/43 (53.49%), Postives = 32/43 (74.42%), Query Frame = 0


Query: 295 SFNGTMNKEDEGNPELKRKQRVATYNMYSLEGKLKKSLRKSFK 338
           S + T+ K    +PE+KRK+RVA Y +YS EGK+K +LRKS+K
Sbjct: 69  SSHSTLFKGWWNDPEIKRKRRVAKYKLYSAEGKMKITLRKSYK 111

The following BLAST results are available for this feature:

BLAST of Carg05282 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7027119.1	1.7e-190	100.00	Crossover junction endonuclease EME1B, partial [Cucurbita argyrosperma subsp. ar...	[more]
KAG6595104.1	3.0e-131	87.11	Crossover junction endonuclease EME1B, partial [Cucurbita argyrosperma subsp. so...	[more]
TYK12984.1	2.3e-54	35.14	crossover junction endonuclease EME1B isoform X1 [Cucumis melo var. makuwa]	[more]
XP_038880967.1	1.2e-52	33.56	crossover junction endonuclease EME1B-like [Benincasa hispida]	[more]
XP_022977604.1	3.6e-52	33.91	crossover junction endonuclease EME1B isoform X3 [Cucurbita maxima]	[more]

BLAST of Carg05282 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
C5H8J1	7.6e-37	57.05	Crossover junction endonuclease EME1B OS=Arabidopsis thaliana OX=3702 GN=EME1B P...	[more]
Q84M98	3.4e-29	49.36	Crossover junction endonuclease EME1A OS=Arabidopsis thaliana OX=3702 GN=EME1A P...	[more]
Q0J9J6	1.7e-25	55.37	Crossover junction endonuclease EME1 OS=Oryza sativa subsp. japonica OX=39947 GN...	[more]

BLAST of Carg05282 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CPM4	1.1e-54	35.14	Crossover junction endonuclease EME1B isoform X1 OS=Cucumis melo var. makuwa OX=...	[more]
A0A6J1IRV2	1.7e-52	33.91	crossover junction endonuclease EME1B isoform X3 OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6J1IQH9	5.1e-52	33.68	crossover junction endonuclease EME1B isoform X2 OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6J1IIZ2	1.1e-51	33.50	crossover junction endonuclease EME1B isoform X1 OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A1S3AZW5	1.5e-51	34.00	crossover junction endonuclease EME1B isoform X2 OS=Cucumis melo OX=3656 GN=LOC1...	[more]

BLAST of Carg05282 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G22140.1	5.4e-38	57.05	essential meiotic endonuclease 1B	[more]
AT2G21800.1	2.4e-30	49.36	essential meiotic endonuclease 1A	[more]
AT2G21800.2	2.4e-30	49.36	essential meiotic endonuclease 1A	[more]
AT4G09890.1	4.2e-06	41.94	Protein of unknown function (DUF3511)	[more]
AT3G05725.1	3.0e-04	53.49	Protein of unknown function (DUF3511)	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021899	Protein of unknown function DUF3511	PFAM	PF12023	DUF3511	coord: 307..337 e-value: 3.1E-14 score: 52.5
IPR042530	EME1/EME2, C-terminal domain	GENE3D	1.10.150.670		coord: 223..293 e-value: 1.8E-7 score: 33.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..23
None	No IPR available	PANTHER	PTHR21077:SF5	METHYL METHANESULFONATE SENSITIVITY 4	coord: 160..327
IPR033310	Mms4/EME1/EME2	PANTHER	PTHR21077	EME1 PROTEIN	coord: 160..327

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg05282-RA	Carg05282-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006281	DNA repair
cellular_component	GO:0048476	Holliday junction resolvase complex
cellular_component	GO:0005634	nucleus