Carg05269 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg05269
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	WRKY domain-containing protein
Location	Carg_Chr07: 3850660 .. 3851845 (-)
RNA-Seq Expression	Carg05269
Synteny	Carg05269

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCATGAACTCCCTTCAAAACCCTACCGACTTTGAAGACTCGTCGACCTCATTCATGGATCTTCTCGATTTCTCGGGTTACCTGCTACCCGATTTCTGGCTCGAACCCGAGTCCACAGTGTTGCCAGAGGCGACAATCGGCGGCCGGAGCAGATCCATGGAAGAAGCAACGTCGACCATGGATAAGAATAGCATGCAAGTGAAAGAGCTTATTATTATATATAGTTAATGAATTAATTTATGTTTGTTAAAATAATTAATGTGTTTTTTTTGCAGAGATGAGTGGCGTGAGAGAAGGGGTGTGAAGAGAAAACAAAAAGGACGAGGTGGTGGGTGCCATAAGGTTGCGTTTAGAACAAAGTCTGAGTTGGAGATCTTGCAGGATGGCTTCAAATGGAGAAAGTATGGCAAAAAATCTATAAAAGATAACCCTAATCCAAGGTAATATTCATATATGCTTGCTTATTTATTCTCCCTTTAAAATAAATTAACGTTTATATAAATATGGTTCCATTAATTAAGATACAGAATAGAGATGTTTAATTGCACATGAGTATTTATGTTAGATTTAGTTTGATTTTTTTTTTTTTCGGTTTGGATTGGGTTAAATTTGTAAAAAAAAACGAAAATTTGGGTTGGTTTGCATGACATTTTTTTGGTTTTGTCAACCCGACCAACCTAGAAATAAGGTTACAACATAGTCTTAGTTTTAAGATCACTGTGAATACTAAGAACACTTGAAGTGATTGTTATGATCCGTGAATGTTTGATATTTGAGTTTTACTACCTCACAACCTAATAGAGAGTTGGTTTGGGTTGAGTTAGGTTGAGTTGTAAACTCTGTTTGCGTTATTCGAATCACCAGTCCAACAAATCCGAAATTTTGGGATAGTCGAAAAAAAAATTCTAACTCAACTCAACCCGTGTACGATCCTACTTTTACATCTTGTGAGGTAGAAAAGAATATGAGTTCCATATTACTATTAACATAATTGAAGAAGCGACTTTTGAAATGATAACCATTGTTTTTATCTAATTTATTTAGGAATTACTACAAATGCTCGAGTACTGGGTGTGGAGTGAAGAAGAGAGTAGAGAGAGACAGAGAAGATTCAAGCTATGTTATAACAACATACGAAGGAATTCACAGCCACGAGAGCCCTTTTCACGAAGTGTTGCAATGA

mRNA sequence

ATGGCCATGAACTCCCTTCAAAACCCTACCGACTTTGAAGACTCGTCGACCTCATTCATGGATCTTCTCGATTTCTCGGGTTACCTGCTACCCGATTTCTGGCTCGAACCCGAGTCCACAGTGTTGCCAGAGGCGACAATCGGCGGCCGGAGCAGATCCATGGAAGAAGCAACAGATGAGTGGCGTGAGAGAAGGGGTGTGAAGAGAAAACAAAAAGGACGAGGTGGTGGGTGCCATAAGGTTGCGTTTAGAACAAAGTCTGAGTTGGAGATCTTGCAGGATGGCTTCAAATGGAGAAAGTATGGCAAAAAATCTATAAAAGATAACCCTAATCCAAGGAATTACTACAAATGCTCGAGTACTGGGTGTGGAGTGAAGAAGAGAGTAGAGAGAGACAGAGAAGATTCAAGCTATGTTATAACAACATACGAAGGAATTCACAGCCACGAGAGCCCTTTTCACGAAGTGTTGCAATGA

Coding sequence (CDS)

ATGGCCATGAACTCCCTTCAAAACCCTACCGACTTTGAAGACTCGTCGACCTCATTCATGGATCTTCTCGATTTCTCGGGTTACCTGCTACCCGATTTCTGGCTCGAACCCGAGTCCACAGTGTTGCCAGAGGCGACAATCGGCGGCCGGAGCAGATCCATGGAAGAAGCAACAGATGAGTGGCGTGAGAGAAGGGGTGTGAAGAGAAAACAAAAAGGACGAGGTGGTGGGTGCCATAAGGTTGCGTTTAGAACAAAGTCTGAGTTGGAGATCTTGCAGGATGGCTTCAAATGGAGAAAGTATGGCAAAAAATCTATAAAAGATAACCCTAATCCAAGGAATTACTACAAATGCTCGAGTACTGGGTGTGGAGTGAAGAAGAGAGTAGAGAGAGACAGAGAAGATTCAAGCTATGTTATAACAACATACGAAGGAATTCACAGCCACGAGAGCCCTTTTCACGAAGTGTTGCAATGA

Protein sequence

MAMNSLQNPTDFEDSSTSFMDLLDFSGYLLPDFWLEPESTVLPEATIGGRSRSMEEATDEWRERRGVKRKQKGRGGGCHKVAFRTKSELEILQDGFKWRKYGKKSIKDNPNPRNYYKCSSTGCGVKKRVERDREDSSYVITTYEGIHSHESPFHEVLQ

Homology

[Close]

BLAST of Carg05269 vs. NCBI nr
Match: KAG7027106.1 (putative WRKY transcription factor 51, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 327.4 bits (838), Expect = 6.8e-86
Identity = 158/158 (100.00%), Postives = 158/158 (100.00%), Query Frame = 0


Query: 1   MAMNSLQNPTDFEDSSTSFMDLLDFSGYLLPDFWLEPESTVLPEATIGGRSRSMEEATDE 60
           MAMNSLQNPTDFEDSSTSFMDLLDFSGYLLPDFWLEPESTVLPEATIGGRSRSMEEATDE
Sbjct: 1   MAMNSLQNPTDFEDSSTSFMDLLDFSGYLLPDFWLEPESTVLPEATIGGRSRSMEEATDE 60

Query: 61  WRERRGVKRKQKGRGGGCHKVAFRTKSELEILQDGFKWRKYGKKSIKDNPNPRNYYKCSS 120
           WRERRGVKRKQKGRGGGCHKVAFRTKSELEILQDGFKWRKYGKKSIKDNPNPRNYYKCSS
Sbjct: 61  WRERRGVKRKQKGRGGGCHKVAFRTKSELEILQDGFKWRKYGKKSIKDNPNPRNYYKCSS 120

Query: 121 TGCGVKKRVERDREDSSYVITTYEGIHSHESPFHEVLQ 159
           TGCGVKKRVERDREDSSYVITTYEGIHSHESPFHEVLQ
Sbjct: 121 TGCGVKKRVERDREDSSYVITTYEGIHSHESPFHEVLQ 158

[Close]

BLAST of Carg05269 vs. NCBI nr
Match: KAG6595091.1 (putative WRKY transcription factor 51, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 315.8 bits (808), Expect = 2.0e-82
Identity = 152/153 (99.35%), Postives = 152/153 (99.35%), Query Frame = 0


Query: 1   MAMNSLQNPTDFEDSSTSFMDLLDFSGYLLPDFWLEPESTVLPEATIGGRSRSMEEATDE 60
           MAMNSLQNPTDFEDSSTSFMDLLDFSGYLLPDFWLEPESTVLPEATIGGRSRSMEEATDE
Sbjct: 1   MAMNSLQNPTDFEDSSTSFMDLLDFSGYLLPDFWLEPESTVLPEATIGGRSRSMEEATDE 60

Query: 61  WRERRGVKRKQKGRGGGCHKVAFRTKSELEILQDGFKWRKYGKKSIKDNPNPRNYYKCSS 120
           WRERRGVKRKQKGRGGGCHKVAFRTKSELEILQDGFKWRKYGKKSIKDNPNPRNYYKCSS
Sbjct: 61  WRERRGVKRKQKGRGGGCHKVAFRTKSELEILQDGFKWRKYGKKSIKDNPNPRNYYKCSS 120

Query: 121 TGCGVKKRVERDREDSSYVITTYEGIHSHESPF 154
           TGCGVKKRVERDR DSSYVITTYEGIHSHESPF
Sbjct: 121 TGCGVKKRVERDRNDSSYVITTYEGIHSHESPF 153

[Close]

BLAST of Carg05269 vs. NCBI nr
Match: XP_022963170.1 (probable WRKY transcription factor 51 [Cucurbita moschata])

HSP 1 Score: 300.8 bits (769), Expect = 6.8e-78
Identity = 148/161 (91.93%), Postives = 149/161 (92.55%), Query Frame = 0


Query: 1   MAMNSLQNPTDFEDSSTSFMDLLDFSGYLLPDFWLEPESTVLPEATIGGRSRSMEEAT-- 60
           MAMNSLQNPTDFEDSSTSFMDLLDFSGYLLPDFWLEPESTVLPEATIG RSRSMEEAT  
Sbjct: 1   MAMNSLQNPTDFEDSSTSFMDLLDFSGYLLPDFWLEPESTVLPEATIGSRSRSMEEATST 60

Query: 61  ------DEWRERRGVKRKQKGRGGGCHKVAFRTKSELEILQDGFKWRKYGKKSIKDNPNP 120
                 DEW ERRGVKRKQKGRGGGCHKVAFRTKSELE+LQDGFKWRKYGKKSIKDNPNP
Sbjct: 61  MDKTSIDEWHERRGVKRKQKGRGGGCHKVAFRTKSELEVLQDGFKWRKYGKKSIKDNPNP 120

Query: 121 RNYYKCSSTGCGVKKRVERDREDSSYVITTYEGIHSHESPF 154
           RNYYKCSS GC VKKRVERDREDSSYVITTYEGIHSHESPF
Sbjct: 121 RNYYKCSSNGCRVKKRVERDREDSSYVITTYEGIHSHESPF 161

[Close]

BLAST of Carg05269 vs. NCBI nr
Match: XP_023518785.1 (probable WRKY transcription factor 51 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 291.6 bits (745), Expect = 4.1e-75
Identity = 142/159 (89.31%), Postives = 147/159 (92.45%), Query Frame = 0


Query: 3   MNSLQNPTDFEDSSTSFMDLLDFSGYLLPDFWLEPESTVLPEATIGGRSRSMEEAT---- 62
           MNSLQNPT FEDSS+SFMDLLDFSGYLLPDFWLEPE+T  PEA IGGR+RSMEEAT    
Sbjct: 1   MNSLQNPTQFEDSSSSFMDLLDFSGYLLPDFWLEPETTAFPEAIIGGRTRSMEEATSTMD 60

Query: 63  ----DEWRERRGVKRKQKGRGGGCHKVAFRTKSELEILQDGFKWRKYGKKSIKDNPNPRN 122
               DEWRERRGVKRKQKGRGGGCHKVAFRTKSELEILQDGFKWRKYGKKSIK+NPNPRN
Sbjct: 61  KNSIDEWRERRGVKRKQKGRGGGCHKVAFRTKSELEILQDGFKWRKYGKKSIKNNPNPRN 120

Query: 123 YYKCSSTGCGVKKRVERDREDSSYVITTYEGIHSHESPF 154
           YYKCSS+GCGVKKRVERDREDSSYVITTYEGIHSHESPF
Sbjct: 121 YYKCSSSGCGVKKRVERDREDSSYVITTYEGIHSHESPF 159

[Close]

BLAST of Carg05269 vs. NCBI nr
Match: XP_023003480.1 (probable WRKY transcription factor 51 [Cucurbita maxima])

HSP 1 Score: 278.9 bits (712), Expect = 2.8e-71
Identity = 138/160 (86.25%), Postives = 143/160 (89.38%), Query Frame = 0


Query: 1   MAMNSLQNPTDFEDSSTSFMDLLDFSGYLLPDFWLEPESTVLPEATIGGRSRSMEEAT-- 60
           MAMNSLQNPT FED S+SFMDLLDFSGY LPDFWLEPE  VLPEATIGG+SRSMEEAT  
Sbjct: 1   MAMNSLQNPTQFEDLSSSFMDLLDFSGYPLPDFWLEPEPAVLPEATIGGQSRSMEEATSR 60

Query: 61  ------DEWRERRGVKRKQKGRGGGCHKVAFRTKSELEILQDGFKWRKYGKKSIKDNPNP 120
                 DEWRERR VKR+Q GRGGGCHKVAFRTKSELEILQDGFKWRKYGKKSIK+NPNP
Sbjct: 61  MDKNSIDEWRERRDVKREQNGRGGGCHKVAFRTKSELEILQDGFKWRKYGKKSIKNNPNP 120

Query: 121 RNYYKCSSTGCGVKKRVERDREDSSYVITTYEGIHSHESP 153
           RNYYKC S+GC VKKRVERDREDSSYVITTYEGIHSHESP
Sbjct: 121 RNYYKCLSSGCRVKKRVERDREDSSYVITTYEGIHSHESP 160

[Close]

BLAST of Carg05269 vs. ExPASy Swiss-Prot
Match: Q93WU9 (Probable WRKY transcription factor 51 OS=Arabidopsis thaliana OX=3702 GN=WRKY51 PE=1 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 9.4e-30
Identity = 80/169 (47.34%), Postives = 101/169 (59.76%), Query Frame = 0


Query: 3   MNSLQNP----TDFEDSS--TSFMDLLDFSGY------------LLPDFWLEPESTVLPE 62
           MN  QNP    T F D +    FMD  DFS              L+ +    P S V  E
Sbjct: 1   MNISQNPSPNFTYFSDENFINPFMDNNDFSNLMFFDIDEGGNNGLIEEEISSPTSIVSSE 60

Query: 63  ATIG--GRSRSMEEATDEWRERRGVKRKQKGRGGGCHKVAFRTKSELEILQDGFKWRKYG 122
              G  G S S    + +    RG K   + +  G H+VAFRT+S+++++ DGFKWRKYG
Sbjct: 61  TFTGESGGSGSATTLSKKESTNRGSKESDQTKETG-HRVAFRTRSKIDVMDDGFKWRKYG 120

Query: 123 KKSIKDNPNPRNYYKCSSTGCGVKKRVERDREDSSYVITTYEGIHSHES 152
           KKS+K+N N RNYYKCSS GC VKKRVERD +D++YVITTYEG+H+HES
Sbjct: 121 KKSVKNNINKRNYYKCSSEGCSVKKRVERDGDDAAYVITTYEGVHNHES 168

[Close]

BLAST of Carg05269 vs. ExPASy Swiss-Prot
Match: Q8VWQ5 (Probable WRKY transcription factor 50 OS=Arabidopsis thaliana OX=3702 GN=WRKY50 PE=2 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 2.8e-26
Identity = 56/89 (62.92%), Postives = 72/89 (80.90%), Query Frame = 0


Query: 63  ERRGVKRKQKGRGGGCHKVAFRTKSELEILQDGFKWRKYGKKSIKDNPNPRNYYKCSSTG 122
           + +  K K+K +G    +VAF+T+SE+E+L DGFKWRKYGKK +K++P+PRNYYKCS  G
Sbjct: 87  DNQNKKEKKKIKG----RVAFKTRSEVEVLDDGFKWRKYGKKMVKNSPHPRNYYKCSVDG 146

Query: 123 CGVKKRVERDREDSSYVITTYEGIHSHES 152
           C VKKRVERDR+D S+VITTYEG H+H S
Sbjct: 147 CPVKKRVERDRDDPSFVITTYEGSHNHSS 171

[Close]

BLAST of Carg05269 vs. ExPASy Swiss-Prot
Match: Q9FGZ4 (Probable WRKY transcription factor 48 OS=Arabidopsis thaliana OX=3702 GN=WRKY48 PE=2 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 1.6e-21
Identity = 49/103 (47.57%), Postives = 73/103 (70.87%), Query Frame = 0


Query: 55  EEATDEWRERRGVKRKQKGRGGGCHKV-----AFRTKSELEILQDGFKWRKYGKKSIKDN 114
           +E  D+ +E++G K + K +     K      AF TKS+++ L DG++WRKYG+K++K++
Sbjct: 178 QEEGDQQQEQKGTKPQLKAKKKNQKKAREARFAFLTKSDIDNLDDGYRWRKYGQKAVKNS 237

Query: 115 PNPRNYYKCSSTGCGVKKRVERDREDSSYVITTYEGIHSHESP 153
           P PR+YY+C++ GCGVKKRVER  +D S V+TTYEG H+H  P
Sbjct: 238 PYPRSYYRCTTVGCGVKKRVERSSDDPSIVMTTYEGQHTHPFP 280

[Close]

BLAST of Carg05269 vs. ExPASy Swiss-Prot
Match: Q6VWJ6 (WRKY transcription factor SUSIBA2 OS=Hordeum vulgare OX=4513 GN=WRKY46 PE=1 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 2.1e-21
Identity = 54/113 (47.79%), Postives = 71/113 (62.83%), Query Frame = 0


Query: 48  GGRSRSMEEATDE--------WRERRGVKRKQKGRGGGCHKVAFRTKSELEILQDGFKWR 107
           GGR    ++AT+E          E  G+     G+     +V  +T SE++IL DG++WR
Sbjct: 310 GGRPYPGDDATEEEDLESKRRKMESAGIDAALMGKPNREPRVVVQTVSEVDILDDGYRWR 369

Query: 108 KYGKKSIKDNPNPRNYYKCSSTGCGVKKRVERDREDSSYVITTYEGIHSHESP 153
           KYG+K +K NPNPR+YYKC+STGC V+K VER   D   VITTYEG H+HE P
Sbjct: 370 KYGQKVVKGNPNPRSYYKCTSTGCPVRKHVERASHDPKSVITTYEGKHNHEVP 422

[Close]

BLAST of Carg05269 vs. ExPASy Swiss-Prot
Match: Q93WV4 (WRKY transcription factor 71 OS=Arabidopsis thaliana OX=3702 GN=WRKY71 PE=2 SV=1)

HSP 1 Score: 101.3 bits (251), Expect = 1.0e-20
Identity = 50/109 (45.87%), Postives = 77/109 (70.64%), Query Frame = 0


Query: 44  EATIGGRSRSMEEATDEWRERRGVKRKQKGRGGGCHKVAFRTKSELEILQDGFKWRKYGK 103
           E  + G   S ++ T + + ++G K++++ R      VAF TKSE++ L+DG++WRKYG+
Sbjct: 94  EGDLHGVGESSKQLTKQGK-KKGEKKEREVR------VAFMTKSEIDHLEDGYRWRKYGQ 153

Query: 104 KSIKDNPNPRNYYKCSSTGCGVKKRVERDREDSSYVITTYEGIHSHESP 153
           K++K++P PR+YY+C++  C VKKRVER  +D S VITTYEG H+H  P
Sbjct: 154 KAVKNSPYPRSYYRCTTQKCNVKKRVERSFQDPSIVITTYEGKHNHPIP 195

[Close]

BLAST of Carg05269 vs. ExPASy TrEMBL
Match: A0A6J1HH83 (probable WRKY transcription factor 51 OS=Cucurbita moschata OX=3662 GN=LOC111463457 PE=4 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 3.3e-78
Identity = 148/161 (91.93%), Postives = 149/161 (92.55%), Query Frame = 0


Query: 1   MAMNSLQNPTDFEDSSTSFMDLLDFSGYLLPDFWLEPESTVLPEATIGGRSRSMEEAT-- 60
           MAMNSLQNPTDFEDSSTSFMDLLDFSGYLLPDFWLEPESTVLPEATIG RSRSMEEAT  
Sbjct: 1   MAMNSLQNPTDFEDSSTSFMDLLDFSGYLLPDFWLEPESTVLPEATIGSRSRSMEEATST 60

Query: 61  ------DEWRERRGVKRKQKGRGGGCHKVAFRTKSELEILQDGFKWRKYGKKSIKDNPNP 120
                 DEW ERRGVKRKQKGRGGGCHKVAFRTKSELE+LQDGFKWRKYGKKSIKDNPNP
Sbjct: 61  MDKTSIDEWHERRGVKRKQKGRGGGCHKVAFRTKSELEVLQDGFKWRKYGKKSIKDNPNP 120

Query: 121 RNYYKCSSTGCGVKKRVERDREDSSYVITTYEGIHSHESPF 154
           RNYYKCSS GC VKKRVERDREDSSYVITTYEGIHSHESPF
Sbjct: 121 RNYYKCSSNGCRVKKRVERDREDSSYVITTYEGIHSHESPF 161

[Close]

BLAST of Carg05269 vs. ExPASy TrEMBL
Match: A0A6J1KRV9 (probable WRKY transcription factor 51 OS=Cucurbita maxima OX=3661 GN=LOC111497082 PE=4 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 1.3e-71
Identity = 138/160 (86.25%), Postives = 143/160 (89.38%), Query Frame = 0


Query: 1   MAMNSLQNPTDFEDSSTSFMDLLDFSGYLLPDFWLEPESTVLPEATIGGRSRSMEEAT-- 60
           MAMNSLQNPT FED S+SFMDLLDFSGY LPDFWLEPE  VLPEATIGG+SRSMEEAT  
Sbjct: 1   MAMNSLQNPTQFEDLSSSFMDLLDFSGYPLPDFWLEPEPAVLPEATIGGQSRSMEEATSR 60

Query: 61  ------DEWRERRGVKRKQKGRGGGCHKVAFRTKSELEILQDGFKWRKYGKKSIKDNPNP 120
                 DEWRERR VKR+Q GRGGGCHKVAFRTKSELEILQDGFKWRKYGKKSIK+NPNP
Sbjct: 61  MDKNSIDEWRERRDVKREQNGRGGGCHKVAFRTKSELEILQDGFKWRKYGKKSIKNNPNP 120

Query: 121 RNYYKCSSTGCGVKKRVERDREDSSYVITTYEGIHSHESP 153
           RNYYKC S+GC VKKRVERDREDSSYVITTYEGIHSHESP
Sbjct: 121 RNYYKCLSSGCRVKKRVERDREDSSYVITTYEGIHSHESP 160

[Close]

BLAST of Carg05269 vs. ExPASy TrEMBL
Match: A0A6J1BUC3 (probable WRKY transcription factor 51 OS=Momordica charantia OX=3673 GN=LOC111005775 PE=4 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 2.6e-51
Identity = 113/165 (68.48%), Postives = 129/165 (78.18%), Query Frame = 0


Query: 3   MNSLQNPT-----DFEDSSTSFMDLLDFSGYLLPDFWL-EPESTVLPEATIGGRSRSM-E 62
           MNS QNP+      F+DSS+SFMD  DFSGY  PDF + E E+  LPEA IG RS  M  
Sbjct: 1   MNSPQNPSCLNHHHFQDSSSSFMDFFDFSGYPPPDFIVEEAETMTLPEARIGRRSIPMTT 60

Query: 63  EAT-------DEWRERRGVKRKQKGRGGGCHKVAFRTKSELEILQDGFKWRKYGKKSIKD 122
           EAT       +EW E +GVKRK++G GG  ++VAFRTKSELEIL DGFKWRKYGKKS+KD
Sbjct: 61  EATFTDNNTIEEWCETKGVKRKKEGGGGCSNRVAFRTKSELEILDDGFKWRKYGKKSVKD 120

Query: 123 NPNPRNYYKCSSTGCGVKKRVERDREDSSYVITTYEGIHSHESPF 154
           +PNPRNYYKCSS GCGVKKRVERDR+DSSYVITTYEG+H+HESPF
Sbjct: 121 SPNPRNYYKCSSGGCGVKKRVERDRDDSSYVITTYEGVHNHESPF 165

[Close]

BLAST of Carg05269 vs. ExPASy TrEMBL
Match: A0A1S3AZZ8 (probable WRKY transcription factor 51 OS=Cucumis melo OX=3656 GN=LOC103484689 PE=4 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 2.2e-50
Identity = 114/169 (67.46%), Postives = 130/169 (76.92%), Query Frame = 0


Query: 3   MNSLQNPTDF--------EDSSTSFMDLLDFSGYLLPDFWLEPESTV--LPEATIGGRSR 62
           MNSLQNPT F        +DSS+SFMD L+FSGY LPDF LE E+T+  L EA  G  SR
Sbjct: 1   MNSLQNPTFFFDHHQQLDQDSSSSFMDFLNFSGYPLPDFGLEAETTMFSLSEAGTGDGSR 60

Query: 63  SMEEAT-------DEWRERRGVKRKQKGRGGGC-HKVAFRTKSELEILQDGFKWRKYGKK 122
           SM+  +       D W E +GVKRK K RG GC HKVAF TKSELEIL DG+KWRKYGKK
Sbjct: 61  SMKATSIDNNTIDDGWFEGKGVKRK-KERGNGCNHKVAFITKSELEILDDGYKWRKYGKK 120

Query: 123 SIKDNPNPRNYYKCSSTGCGVKKRVERDREDSSYVITTYEGIHSHESPF 154
           S+K++P+PRNYYKCSS GCGVKKRVERDR+DSSYVITTYEG+H+HESPF
Sbjct: 121 SVKNSPHPRNYYKCSSGGCGVKKRVERDRDDSSYVITTYEGVHNHESPF 168

[Close]

BLAST of Carg05269 vs. ExPASy TrEMBL
Match: A0A0A0KFX2 (WRKY domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G486960 PE=4 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 2.8e-45
Identity = 105/169 (62.13%), Postives = 121/169 (71.60%), Query Frame = 0


Query: 3   MNSLQNPTDFED---------SSTSFMDLLDFSGYLLPDFWLEPESTV--LPEATIGGRS 62
           MNSLQNP  F D         SS+S MD L+FSGY LPDF LE E+T   L EA  G  S
Sbjct: 1   MNSLQNPNFFFDHHQQFDQDHSSSSIMDFLNFSGYPLPDFGLEAETTTFSLSEAETGDGS 60

Query: 63  RSMEEAT-------DEWRERRGVKRKQKGRGGGCHKVAFRTKSELEILQDGFKWRKYGKK 122
            SM+  +       D W E +GVKRK+    G  ++VAF TKSELEIL DGFKWRKYGKK
Sbjct: 61  GSMKATSIDNNTIDDGWFEGKGVKRKKPRENGRTNRVAFITKSELEILDDGFKWRKYGKK 120

Query: 123 SIKDNPNPRNYYKCSSTGCGVKKRVERDREDSSYVITTYEGIHSHESPF 154
           S+K++P+PRNYYKCSS  CGVKKRVERDR+DSSYVITTYEG+H+HESPF
Sbjct: 121 SVKNSPHPRNYYKCSSGECGVKKRVERDRDDSSYVITTYEGVHNHESPF 169

[Close]

BLAST of Carg05269 vs. TAIR 10
Match: AT5G64810.1 (WRKY DNA-binding protein 51 )

HSP 1 Score: 131.3 bits (329), Expect = 6.6e-31
Identity = 80/169 (47.34%), Postives = 101/169 (59.76%), Query Frame = 0


Query: 3   MNSLQNP----TDFEDSS--TSFMDLLDFSGY------------LLPDFWLEPESTVLPE 62
           MN  QNP    T F D +    FMD  DFS              L+ +    P S V  E
Sbjct: 1   MNISQNPSPNFTYFSDENFINPFMDNNDFSNLMFFDIDEGGNNGLIEEEISSPTSIVSSE 60

Query: 63  ATIG--GRSRSMEEATDEWRERRGVKRKQKGRGGGCHKVAFRTKSELEILQDGFKWRKYG 122
              G  G S S    + +    RG K   + +  G H+VAFRT+S+++++ DGFKWRKYG
Sbjct: 61  TFTGESGGSGSATTLSKKESTNRGSKESDQTKETG-HRVAFRTRSKIDVMDDGFKWRKYG 120

Query: 123 KKSIKDNPNPRNYYKCSSTGCGVKKRVERDREDSSYVITTYEGIHSHES 152
           KKS+K+N N RNYYKCSS GC VKKRVERD +D++YVITTYEG+H+HES
Sbjct: 121 KKSVKNNINKRNYYKCSSEGCSVKKRVERDGDDAAYVITTYEGVHNHES 168

[Close]

BLAST of Carg05269 vs. TAIR 10
Match: AT5G26170.1 (WRKY DNA-binding protein 50 )

HSP 1 Score: 119.8 bits (299), Expect = 2.0e-27
Identity = 56/89 (62.92%), Postives = 72/89 (80.90%), Query Frame = 0


Query: 63  ERRGVKRKQKGRGGGCHKVAFRTKSELEILQDGFKWRKYGKKSIKDNPNPRNYYKCSSTG 122
           + +  K K+K +G    +VAF+T+SE+E+L DGFKWRKYGKK +K++P+PRNYYKCS  G
Sbjct: 87  DNQNKKEKKKIKG----RVAFKTRSEVEVLDDGFKWRKYGKKMVKNSPHPRNYYKCSVDG 146

Query: 123 CGVKKRVERDREDSSYVITTYEGIHSHES 152
           C VKKRVERDR+D S+VITTYEG H+H S
Sbjct: 147 CPVKKRVERDRDDPSFVITTYEGSHNHSS 171

[Close]

BLAST of Carg05269 vs. TAIR 10
Match: AT5G49520.1 (WRKY DNA-binding protein 48 )

HSP 1 Score: 104.0 bits (258), Expect = 1.1e-22
Identity = 49/103 (47.57%), Postives = 73/103 (70.87%), Query Frame = 0


Query: 55  EEATDEWRERRGVKRKQKGRGGGCHKV-----AFRTKSELEILQDGFKWRKYGKKSIKDN 114
           +E  D+ +E++G K + K +     K      AF TKS+++ L DG++WRKYG+K++K++
Sbjct: 178 QEEGDQQQEQKGTKPQLKAKKKNQKKAREARFAFLTKSDIDNLDDGYRWRKYGQKAVKNS 237

Query: 115 PNPRNYYKCSSTGCGVKKRVERDREDSSYVITTYEGIHSHESP 153
           P PR+YY+C++ GCGVKKRVER  +D S V+TTYEG H+H  P
Sbjct: 238 PYPRSYYRCTTVGCGVKKRVERSSDDPSIVMTTYEGQHTHPFP 280

[Close]

BLAST of Carg05269 vs. TAIR 10
Match: AT1G29860.1 (WRKY DNA-binding protein 71 )

HSP 1 Score: 101.3 bits (251), Expect = 7.4e-22
Identity = 50/109 (45.87%), Postives = 77/109 (70.64%), Query Frame = 0


Query: 44  EATIGGRSRSMEEATDEWRERRGVKRKQKGRGGGCHKVAFRTKSELEILQDGFKWRKYGK 103
           E  + G   S ++ T + + ++G K++++ R      VAF TKSE++ L+DG++WRKYG+
Sbjct: 94  EGDLHGVGESSKQLTKQGK-KKGEKKEREVR------VAFMTKSEIDHLEDGYRWRKYGQ 153

Query: 104 KSIKDNPNPRNYYKCSSTGCGVKKRVERDREDSSYVITTYEGIHSHESP 153
           K++K++P PR+YY+C++  C VKKRVER  +D S VITTYEG H+H  P
Sbjct: 154 KAVKNSPYPRSYYRCTTQKCNVKKRVERSFQDPSIVITTYEGKHNHPIP 195

[Close]

BLAST of Carg05269 vs. TAIR 10
Match: AT2G47260.1 (WRKY DNA-binding protein 23 )

HSP 1 Score: 100.1 bits (248), Expect = 1.6e-21
Identity = 48/103 (46.60%), Postives = 72/103 (69.90%), Query Frame = 0


Query: 55  EEATDEWRERRGVKRKQKGRGGGCH-----KVAFRTKSELEILQDGFKWRKYGKKSIKDN 114
           EE  ++ +E+   K++ K +          +VAF TKSE++ L+DG++WRKYG+K++K++
Sbjct: 131 EEGGEDQQEKSHTKKQLKAKKNNQKRQREARVAFMTKSEVDHLEDGYRWRKYGQKAVKNS 190

Query: 115 PNPRNYYKCSSTGCGVKKRVERDREDSSYVITTYEGIHSHESP 153
           P PR+YY+C++  C VKKRVER   D S V+TTYEG H+H SP
Sbjct: 191 PFPRSYYRCTTASCNVKKRVERSFRDPSTVVTTYEGQHTHISP 233

The following BLAST results are available for this feature:

BLAST of Carg05269 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7027106.1	6.8e-86	100.00	putative WRKY transcription factor 51, partial [Cucurbita argyrosperma subsp. ar...	[more]
KAG6595091.1	2.0e-82	99.35	putative WRKY transcription factor 51, partial [Cucurbita argyrosperma subsp. so...	[more]
XP_022963170.1	6.8e-78	91.93	probable WRKY transcription factor 51 [Cucurbita moschata]	[more]
XP_023518785.1	4.1e-75	89.31	probable WRKY transcription factor 51 [Cucurbita pepo subsp. pepo]	[more]
XP_023003480.1	2.8e-71	86.25	probable WRKY transcription factor 51 [Cucurbita maxima]	[more]

BLAST of Carg05269 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93WU9	9.4e-30	47.34	Probable WRKY transcription factor 51 OS=Arabidopsis thaliana OX=3702 GN=WRKY51 ...	[more]
Q8VWQ5	2.8e-26	62.92	Probable WRKY transcription factor 50 OS=Arabidopsis thaliana OX=3702 GN=WRKY50 ...	[more]
Q9FGZ4	1.6e-21	47.57	Probable WRKY transcription factor 48 OS=Arabidopsis thaliana OX=3702 GN=WRKY48 ...	[more]
Q6VWJ6	2.1e-21	47.79	WRKY transcription factor SUSIBA2 OS=Hordeum vulgare OX=4513 GN=WRKY46 PE=1 SV=1	[more]
Q93WV4	1.0e-20	45.87	WRKY transcription factor 71 OS=Arabidopsis thaliana OX=3702 GN=WRKY71 PE=2 SV=1	[more]

BLAST of Carg05269 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HH83	3.3e-78	91.93	probable WRKY transcription factor 51 OS=Cucurbita moschata OX=3662 GN=LOC111463...	[more]
A0A6J1KRV9	1.3e-71	86.25	probable WRKY transcription factor 51 OS=Cucurbita maxima OX=3661 GN=LOC11149708...	[more]
A0A6J1BUC3	2.6e-51	68.48	probable WRKY transcription factor 51 OS=Momordica charantia OX=3673 GN=LOC11100...	[more]
A0A1S3AZZ8	2.2e-50	67.46	probable WRKY transcription factor 51 OS=Cucumis melo OX=3656 GN=LOC103484689 PE...	[more]
A0A0A0KFX2	2.8e-45	62.13	WRKY domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G486960 PE=4 S...	[more]

BLAST of Carg05269 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G64810.1	6.6e-31	47.34	WRKY DNA-binding protein 51	[more]
AT5G26170.1	2.0e-27	62.92	WRKY DNA-binding protein 50	[more]
AT5G49520.1	1.1e-22	47.57	WRKY DNA-binding protein 48	[more]
AT1G29860.1	7.4e-22	45.87	WRKY DNA-binding protein 71	[more]
AT2G47260.1	1.6e-21	46.60	WRKY DNA-binding protein 23	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003657	WRKY domain	SMART	SM00774	WRKY_cls	coord: 92..151 e-value: 1.3E-32 score: 124.4
IPR003657	WRKY domain	PFAM	PF03106	WRKY	coord: 93..150 e-value: 7.3E-23 score: 80.6
IPR003657	WRKY domain	PROSITE	PS50811	WRKY	coord: 87..152 score: 30.216612
IPR036576	WRKY domain superfamily	GENE3D	2.20.25.80	WRKY domain	coord: 79..145 e-value: 3.9E-31 score: 108.6
IPR036576	WRKY domain superfamily	SUPERFAMILY	118290	WRKY DNA-binding domain	coord: 85..152
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 49..77
None	No IPR available	PANTHER	PTHR31221:SF219	WRKY TRANSCRIPTION FACTOR 50-RELATED	coord: 54..152
IPR044810	WRKY transcription factor, plant	PANTHER	PTHR31221	WRKY TRANSCRIPTION FACTOR PROTEIN 1-RELATED	coord: 54..152

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg05269-RA	Carg05269-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003700	DNA-binding transcription factor activity
molecular_function	GO:0043565	sequence-specific DNA binding