Carg05233 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg05233
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	salicylic acid-binding protein 2-like
Location	Carg_Chr07: 3670594 .. 3673169 (+)
RNA-Seq Expression	Carg05233
Synteny	Carg05233

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACTTGTAGCCACCAAGAGTTTGGCAAGTCCTCTACGTTCAACCTGAACATAATTTTTGGAAGAGACTTGACTCAAATGGAGCAAAAGCATTTTGTATTTGTCCACGGAGCTTGCCATGGAGCTTGGAGCTGGTTCAAGATCAAGCCCCTGCTCGAGGCTGCTGGCCACCGTGTAACGCTGCTGGACATGGCTGCCTCGGGCATCCACAGGAAGGTCATCCAAGATGTGCACTCCATTGTAGACTACTCTGAGCCTTTGTTGAAGGTCATGGCCTGTCTTCCTCCAAATCAAAAGGTGATATTGGTGGGTCATAGCCTTGGCGGCTTCAACTTGGCTGTGGCTATGGACAGGTTCCCCGACAAGATAGCTGCCTCTGTTTTCGTGGCGGCCTTTGTCCCTGACACTGACCACAAGCCTTCCTATGTCCTTGATCAGGTACCTATTACTTTTACCGTCTAATAAAAAATTTGATTACAGAGCATTCAAAAGAATCTATTCTAAATTTTTAAACAATAAAGGATGAATCTTTGGATGACCCAAAACTCAAATTTTGTGTTGATGCAAGTACAATGAGCAAACCCCCATGGAAGCTTGGCTCGACACTCAGTTTAGTCCTTATGGAAACGAAGCTAAGCCTTCAACATCAATGTTTTTCGGACCCGATTTCGTGGCCAAGAAGCTCTACCAACTCTCCCCTCCCGAGGTTTCTCTTTCTTTATCAGTTACATTTATTAAAGATATTTAGTATTGAATTATAGTTTATCATATACATCAGGATTTAGCACTCGGGCTCAGTCTTCTAAGGCCAAGTTCATTGTTCATGCAAGACTTGTCAAAGGCAAACAATTTTTCCAAGCAAAACTATGGATCCGTGAAGAAAGTATACATCGTTTGTACAGAAGACAAAGGAATCCCAGAGGAGTTCCAAAAGTGGATGATCCACAACGGTGGGATTGAAAATGTGATGGAAATAAACGGCTCTGATCACATGCCCATGTTTTGCAAGCCTCGCCAGCTTCTCCACTGTCTCCTTCAGGTTGCTGTCAGCTGCGCCTAATCCAGATTTTACTTCCAACGCCTCTGAAATAAATGAGACGGAAGGCAATAATAATAAATGTTTTATAATTCTGCAAGACTTTCTAAATGTTGGGTTTGGGATGTTATGATATGGAGTGCTGCTTGGTTCATTATTGGGAATGCATGTCAATGTGTTTCTTTCAGTACCTAAAACTTGTTATTTACCATATCTTTTATAATTCTGTACGTCTTCCTTAATTTGGGGATTGTGATTTTATGATATGATCCATGATTGGGCATGCGTGTCAGTTTGTCTATGATATCCATCTTATATCCAAGGTGTTACTATAATATGCGCCAAAACAGTTCATACCATCCATTTATATACCATCAAGGAAATAGCATAAATAGAATTAAAAAAAAAAAAAAAAAAAAAAAACTCAACTAACAAAAACACTATATCTGATCCAAGGGCTATGATTTGAAGATAAATGCACCTATCCCAGCGTAAATGTATGGTGCAAGTTGTATATTCGTTCTTTCATGAAAACATACGTGCGTAACGGTATTTCATAATTTTGAAGTAATCATGATATGCGTAACATATCATTTAGAGGTATTATGTATCATACGACATGATATGCATAAATAATTCTCCTATTGACTCCACCGACTATCAAGAATCTTTGTGACTCAATTAAATCTAGGGACCTTGCTCTTATGTTCAAGTTCAACTATTCTTGATTTAACTGATTTGGGGCAACCTCTTTGCTTCCACAAAACCTCCAAAATCCTCAAATATCCCATCATAATTAGGATCTAGGTTTGGCAAAGGCATATTGTTGGGCTTTGTACTTTTGTTCGAAAGCCTGCATCAACGAACAACGTGGTACCCTTGGTCTGATGTTGGACATTTATCACCTCTTCTTTGTCAACCTTACGTTTTTGTTCTTCTGTTCTTTGTTTTTTCTATTGCAAGGTCTATTTCTTCATTTGTCCCCCAACTTAAAACAAATTTCTTTGTTTCAACTAAGTTTTGTCGTTGTAAAATCTTCAACTCTTTACACATCTCAATAATATTGTCACTGTCAATCTCTGCCTCGATCACATGGAGCACAGGCATTTTGTATTGGTCCATGGGGCTTGCCATGGAGCATGGACCTGGTACAATATCAAGCCGCTTGGCTGTGGCCATGGAGAAGTTCCCTGCAAAGATAGAAGCTTCTGTTTTCTTGACAGCCTTTGCTCCTGATACCCACCACAACCCTTCTTATGTCTTAGATCAGGTAAATATATACTCATCTGTTTCTTGTTTGTTTATATTGAAGTTCCTGGAGAGAGCTCCCAAGGGATTTTGGCTGGACACCCAGTTTTCTTCTTATGAAACCACAGCAGCAGATGAACCTTTAACTTCATTTTTGTTTGGCCCCGAATGCATGGCCAAGAACCTCTATCAGCTCTCCCCTTTCTTTTTTTCCCATAGTTCTCCTAAATGGATTTTGATTGATTTTTCCACAACCAAAAAAGCCTCTCCAAGTTTTCAAGCGTCTTCTTCA

mRNA sequence

ATGACTTGTAGCCACCAAGAGTTTGGCAAGTCCTCTACGTTCAACCTGAACATAATTTTTGGAAGAGACTTGACTCAAATGGAGCAAAAGCATTTTGTATTTGTCCACGGAGCTTGCCATGGAGCTTGGAGCTGGTTCAAGATCAAGCCCCTGCTCGAGGCTGCTGGCCACCGTGTAACGCTGCTGGACATGGCTGCCTCGGGCATCCACAGGAAGGTCATCCAAGATGTGCACTCCATTGTAGACTACTCTGAGCCTTTGTTGAAGGTCATGGCCTGTCTTCCTCCAAATCAAAAGGTGATATTGGTGGGTCATAGCCTTGGCGGCTTCAACTTGGCTGTGGCTATGGACAGGTTCCCCGACAAGATAGCTGCCTCTGTTTTCGTGGCGGCCTTTGTCCCTGACACTGACCACAAGCCTTCCTATGTCCTTGATCAGCAAAACTATGGATCCGTGAAGAAAGTATACATCGTTTGTACAGAAGACAAAGGAATCCCAGAGGAGTTCCAAAAGTGGATGATCCACAACGGTGGGATTGAAAATGTGATGGAAATAAACGGCTCTGATCACATGCCCATGTTTTGCAAGCCTCGCCAGCTTCTCCACTGTCTCCTTCAGCATGGACCTGGTACAATATCAAGCCGCTTGGCTGTGGCCATGGAGAAGTTCCCTGCAAAGATAGAAGCTTCTGTTTTCTTGACAGCCTTTGCTCCTGATACCCACCACAACCCTTCTTATGTCTTAGATCAGTTCCTGGAGAGAGCTCCCAAGGGATTTTGGCTGGACACCCAGTTTTCTTCTTATGAAACCACAGCAGCAGATGAACCTTTAACTTCATTTTTGTTTGGCCCCGAATGCATGGCCAAGAACCTCTATCAGCTCTCCCCTTTCTTTTTTTCCCATAGTTCTCCTAAATGGATTTTGATTGATTTTTCCACAACCAAAAAAGCCTCTCCAAGTTTTCAAGCGTCTTCTTCA

Coding sequence (CDS)

ATGACTTGTAGCCACCAAGAGTTTGGCAAGTCCTCTACGTTCAACCTGAACATAATTTTTGGAAGAGACTTGACTCAAATGGAGCAAAAGCATTTTGTATTTGTCCACGGAGCTTGCCATGGAGCTTGGAGCTGGTTCAAGATCAAGCCCCTGCTCGAGGCTGCTGGCCACCGTGTAACGCTGCTGGACATGGCTGCCTCGGGCATCCACAGGAAGGTCATCCAAGATGTGCACTCCATTGTAGACTACTCTGAGCCTTTGTTGAAGGTCATGGCCTGTCTTCCTCCAAATCAAAAGGTGATATTGGTGGGTCATAGCCTTGGCGGCTTCAACTTGGCTGTGGCTATGGACAGGTTCCCCGACAAGATAGCTGCCTCTGTTTTCGTGGCGGCCTTTGTCCCTGACACTGACCACAAGCCTTCCTATGTCCTTGATCAGCAAAACTATGGATCCGTGAAGAAAGTATACATCGTTTGTACAGAAGACAAAGGAATCCCAGAGGAGTTCCAAAAGTGGATGATCCACAACGGTGGGATTGAAAATGTGATGGAAATAAACGGCTCTGATCACATGCCCATGTTTTGCAAGCCTCGCCAGCTTCTCCACTGTCTCCTTCAGCATGGACCTGGTACAATATCAAGCCGCTTGGCTGTGGCCATGGAGAAGTTCCCTGCAAAGATAGAAGCTTCTGTTTTCTTGACAGCCTTTGCTCCTGATACCCACCACAACCCTTCTTATGTCTTAGATCAGTTCCTGGAGAGAGCTCCCAAGGGATTTTGGCTGGACACCCAGTTTTCTTCTTATGAAACCACAGCAGCAGATGAACCTTTAACTTCATTTTTGTTTGGCCCCGAATGCATGGCCAAGAACCTCTATCAGCTCTCCCCTTTCTTTTTTTCCCATAGTTCTCCTAAATGGATTTTGATTGATTTTTCCACAACCAAAAAAGCCTCTCCAAGTTTTCAAGCGTCTTCTTCA

Protein sequence

MTCSHQEFGKSSTFNLNIIFGRDLTQMEQKHFVFVHGACHGAWSWFKIKPLLEAAGHRVTLLDMAASGIHRKVIQDVHSIVDYSEPLLKVMACLPPNQKVILVGHSLGGFNLAVAMDRFPDKIAASVFVAAFVPDTDHKPSYVLDQQNYGSVKKVYIVCTEDKGIPEEFQKWMIHNGGIENVMEINGSDHMPMFCKPRQLLHCLLQHGPGTISSRLAVAMEKFPAKIEASVFLTAFAPDTHHNPSYVLDQFLERAPKGFWLDTQFSSYETTAADEPLTSFLFGPECMAKNLYQLSPFFFSHSSPKWILIDFSTTKKASPSFQASSS

Homology

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BLAST of Carg05233 vs. NCBI nr
Match: KAG7027073.1 (Methylesterase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 680.6 bits (1755), Expect = 6.5e-192
Identity = 326/326 (100.00%), Postives = 326/326 (100.00%), Query Frame = 0


Query: 1   MTCSHQEFGKSSTFNLNIIFGRDLTQMEQKHFVFVHGACHGAWSWFKIKPLLEAAGHRVT 60
           MTCSHQEFGKSSTFNLNIIFGRDLTQMEQKHFVFVHGACHGAWSWFKIKPLLEAAGHRVT
Sbjct: 1   MTCSHQEFGKSSTFNLNIIFGRDLTQMEQKHFVFVHGACHGAWSWFKIKPLLEAAGHRVT 60

Query: 61  LLDMAASGIHRKVIQDVHSIVDYSEPLLKVMACLPPNQKVILVGHSLGGFNLAVAMDRFP 120
           LLDMAASGIHRKVIQDVHSIVDYSEPLLKVMACLPPNQKVILVGHSLGGFNLAVAMDRFP
Sbjct: 61  LLDMAASGIHRKVIQDVHSIVDYSEPLLKVMACLPPNQKVILVGHSLGGFNLAVAMDRFP 120

Query: 121 DKIAASVFVAAFVPDTDHKPSYVLDQQNYGSVKKVYIVCTEDKGIPEEFQKWMIHNGGIE 180
           DKIAASVFVAAFVPDTDHKPSYVLDQQNYGSVKKVYIVCTEDKGIPEEFQKWMIHNGGIE
Sbjct: 121 DKIAASVFVAAFVPDTDHKPSYVLDQQNYGSVKKVYIVCTEDKGIPEEFQKWMIHNGGIE 180

Query: 181 NVMEINGSDHMPMFCKPRQLLHCLLQHGPGTISSRLAVAMEKFPAKIEASVFLTAFAPDT 240
           NVMEINGSDHMPMFCKPRQLLHCLLQHGPGTISSRLAVAMEKFPAKIEASVFLTAFAPDT
Sbjct: 181 NVMEINGSDHMPMFCKPRQLLHCLLQHGPGTISSRLAVAMEKFPAKIEASVFLTAFAPDT 240

Query: 241 HHNPSYVLDQFLERAPKGFWLDTQFSSYETTAADEPLTSFLFGPECMAKNLYQLSPFFFS 300
           HHNPSYVLDQFLERAPKGFWLDTQFSSYETTAADEPLTSFLFGPECMAKNLYQLSPFFFS
Sbjct: 241 HHNPSYVLDQFLERAPKGFWLDTQFSSYETTAADEPLTSFLFGPECMAKNLYQLSPFFFS 300

Query: 301 HSSPKWILIDFSTTKKASPSFQASSS 327
           HSSPKWILIDFSTTKKASPSFQASSS
Sbjct: 301 HSSPKWILIDFSTTKKASPSFQASSS 326

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BLAST of Carg05233 vs. NCBI nr
Match: XP_022962696.1 (salicylic acid-binding protein 2-like [Cucurbita moschata])

HSP 1 Score: 399.1 bits (1024), Expect = 3.8e-107
Identity = 203/279 (72.76%), Postives = 204/279 (73.12%), Query Frame = 0


Query: 1   MTCSHQEFGKSSTFNLNIIFGRDLTQMEQKHFVFVHGACHGAWSWFKIKPLLEAAGHRVT 60
           MTCSHQEFGKSSTFNLNIIFGRDLTQMEQKHFVFVHGACHGAWSWFKIKPLLEAAGHRVT
Sbjct: 1   MTCSHQEFGKSSTFNLNIIFGRDLTQMEQKHFVFVHGACHGAWSWFKIKPLLEAAGHRVT 60

Query: 61  LLDMAASGIHRKVIQDVHSIVDYSEPLLKVMACLPPNQKVILVGHSLGGFNLAVAMDRFP 120
           LLDMAASGIHRKVIQDVHSIVDYSEPLLKVMACLPPNQKVILVGHSLGGFNLAVAM+RFP
Sbjct: 61  LLDMAASGIHRKVIQDVHSIVDYSEPLLKVMACLPPNQKVILVGHSLGGFNLAVAMERFP 120

Query: 121 DKIAASVFVAAFVPDTDHKPSYVLDQ---------------------------------- 180
           DKIAASVFVAAFVPDTDHKPSYVLDQ                                  
Sbjct: 121 DKIAASVFVAAFVPDTDHKPSYVLDQYNEQTPMEAWLDTQFSPYGNEAKPSTSMFFGPDF 180

Query: 181 ---------------------------------------QNYGSVKKVYIVCTEDKGIPE 207
                                                  QNYGSVKKVYIVCTED GIPE
Sbjct: 181 VAKKLYQLSPPEDLALGLSLVRPSSLFMQDLSKANNFSKQNYGSVKKVYIVCTEDIGIPE 240

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BLAST of Carg05233 vs. NCBI nr
Match: KAG6595055.1 (Salicylic acid-binding protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 351.3 bits (900), Expect = 9.1e-93
Identity = 180/253 (71.15%), Postives = 180/253 (71.15%), Query Frame = 0


Query: 27  MEQKHFVFVHGACHGAWSWFKIKPLLEAAGHRVTLLDMAASGIHRKVIQDVHSIVDYSEP 86
           MEQKHFVFVHGACHGAWSWFKIKPLLEAAGHRVTLLDMAASGIHRKVIQDVHSIVDYSEP
Sbjct: 1   MEQKHFVFVHGACHGAWSWFKIKPLLEAAGHRVTLLDMAASGIHRKVIQDVHSIVDYSEP 60

Query: 87  LLKVMACLPPNQKVILVGHSLGGFNLAVAMDRFPDKIAASVFVAAFVPDTDHKPSYVLDQ 146
           LLKVMACLPPNQKVILVGHSLGGFNLAVAMDRFPDKIAASVFVAAFVPDTDHKPSYVLDQ
Sbjct: 61  LLKVMACLPPNQKVILVGHSLGGFNLAVAMDRFPDKIAASVFVAAFVPDTDHKPSYVLDQ 120

Query: 147 ------------------------------------------------------------ 206
                                                                       
Sbjct: 121 YNEQTPMEAWLDTQFSPYGNEAKPSTSMFFGPDFVAKKLYQLSPPEDLALGLSLLRPSSL 180

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BLAST of Carg05233 vs. NCBI nr
Match: XP_023003844.1 (salicylic acid-binding protein 2-like [Cucurbita maxima])

HSP 1 Score: 340.5 bits (872), Expect = 1.6e-89
Identity = 175/253 (69.17%), Postives = 177/253 (69.96%), Query Frame = 0


Query: 27  MEQKHFVFVHGACHGAWSWFKIKPLLEAAGHRVTLLDMAASGIHRKVIQDVHSIVDYSEP 86
           MEQKHFVFVHGACHGAWSWFKIKPLLEAAGHRVTLLDMAASGIHRKVIQDVHSIVDYSEP
Sbjct: 1   MEQKHFVFVHGACHGAWSWFKIKPLLEAAGHRVTLLDMAASGIHRKVIQDVHSIVDYSEP 60

Query: 87  LLKVMACLPPNQKVILVGHSLGGFNLAVAMDRFPDKIAASVFVAAFVPDTDHKPSYVLDQ 146
           LLK MACLPPNQKVILVGHSLGGFNLAVAM+RFPDKIAASVFVAAFVPDT+HKPSYVLDQ
Sbjct: 61  LLKAMACLPPNQKVILVGHSLGGFNLAVAMERFPDKIAASVFVAAFVPDTNHKPSYVLDQ 120

Query: 147 ------------------------------------------------------------ 206
                                                                       
Sbjct: 121 YNEQTPLEAWLDTQFSPYGTEAEPSTSMFFGPNFVAKKLYQLSPPEDLALGLSLLRPSSL 180

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BLAST of Carg05233 vs. NCBI nr
Match: XP_023518216.1 (salicylic acid-binding protein 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 335.1 bits (858), Expect = 6.7e-88
Identity = 172/253 (67.98%), Postives = 174/253 (68.77%), Query Frame = 0


Query: 27  MEQKHFVFVHGACHGAWSWFKIKPLLEAAGHRVTLLDMAASGIHRKVIQDVHSIVDYSEP 86
           MEQKHFV VHGACHGAW WFKIKPLLEAAGHRVTLLDMAASGIHRKVIQDVHSIVDYSEP
Sbjct: 1   MEQKHFVLVHGACHGAWCWFKIKPLLEAAGHRVTLLDMAASGIHRKVIQDVHSIVDYSEP 60

Query: 87  LLKVMACLPPNQKVILVGHSLGGFNLAVAMDRFPDKIAASVFVAAFVPDTDHKPSYVLDQ 146
           LLK MACLPPNQK+ILVGHSLGGFNLAVAMDRFPDKIAASVFVAAFVPDT+H PSYVLDQ
Sbjct: 61  LLKFMACLPPNQKLILVGHSLGGFNLAVAMDRFPDKIAASVFVAAFVPDTNHMPSYVLDQ 120

Query: 147 ------------------------------------------------------------ 206
                                                                       
Sbjct: 121 YNEQTPMEAWLDTQFSPYGNEAEPSTSMFFGPNFLAKKLYQLSPPEDLALGLSLLRPSSL 180

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BLAST of Carg05233 vs. ExPASy Swiss-Prot
Match: Q6RYA0 (Salicylic acid-binding protein 2 OS=Nicotiana tabacum OX=4097 GN=SABP2 PE=1 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 3.0e-54
Identity = 108/252 (42.86%), Postives = 144/252 (57.14%), Query Frame = 0


Query: 28  EQKHFVFVHGACHGAWSWFKIKPLLEAAGHRVTLLDMAASGIHRKVIQDVHSIVDYSEPL 87
           E KHFV VHGACHG WSW+K+KPLLEAAGH+VT LD+AASG   + I+++ ++ DY+ PL
Sbjct: 3   EGKHFVLVHGACHGGWSWYKLKPLLEAAGHKVTALDLAASGTDLRKIEELRTLYDYTLPL 62

Query: 88  LKVMACLPPNQKVILVGHSLGGFNLAVAMDRFPDKIAASVFVAAFVPDTDHKPSYVLDQQ 147
           +++M  L  ++KVILVGHSLGG NL +AM+++P KI A+VF+AAF+PD+ H  S+VL+Q 
Sbjct: 63  MELMESLSADEKVILVGHSLGGMNLGLAMEKYPQKIYAAVFLAAFMPDSVHNSSFVLEQY 122

Query: 148 N----------------------------------------------------------- 207
           N                                                           
Sbjct: 123 NERTPAENWLDTQFLPYGSPEEPLTSMFFGPKFLAHKLYQLCSPEDLALASSLVRPSSLF 182

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BLAST of Carg05233 vs. ExPASy Swiss-Prot
Match: Q8S8S9 (Methylesterase 1 OS=Arabidopsis thaliana OX=3702 GN=MES1 PE=1 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 5.6e-53
Identity = 105/248 (42.34%), Postives = 140/248 (56.45%), Query Frame = 0


Query: 26  QMEQKHFVFVHGACHGAWSWFKIKPLLEAAGHRVTLLDMAASGIH-RKVIQDVHSIVDYS 85
           +  ++HFV VHG+CHGAW W+K+KPLLEA GHRVT +D+AASGI   + I D+ +   YS
Sbjct: 4   EKRKQHFVLVHGSCHGAWCWYKVKPLLEAVGHRVTAVDLAASGIDTTRSITDIPTCEQYS 63

Query: 86  EPLLKVMACLPPNQKVILVGHSLGGFNLAVAMDRFPDKIAASVFVAAFVPDTDHKPSYVL 145
           EPL K++  LP ++KV+LVGHS GG NLA+AM++FP+KI+ +VF+ AF+PDT+H PS+VL
Sbjct: 64  EPLTKLLTSLPNDEKVVLVGHSFGGLNLAIAMEKFPEKISVAVFLTAFMPDTEHSPSFVL 123

Query: 146 DQ---------------------------------------------------------- 201
           D+                                                          
Sbjct: 124 DKFGSNMPQEAWMGTEFEPYGSDNSGLSMFFSPDFMKLGLYQLSPVEDLELGLLLMRPGS 183

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BLAST of Carg05233 vs. ExPASy Swiss-Prot
Match: O80476 (Methylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=MES2 PE=1 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 1.2e-50
Identity = 106/253 (41.90%), Postives = 136/253 (53.75%), Query Frame = 0


Query: 26  QMEQKHFVFVHGACHGAWSWFKIKPLLEAAGHRVTLLDMAASGIH-RKVIQDVHSIVDYS 85
           +  ++HFV VHGACHGAW W+K+KPLLEA GHRVT LD+AASGI   + I D+ +   YS
Sbjct: 4   EKRKQHFVLVHGACHGAWCWYKVKPLLEALGHRVTALDLAASGIDTTRSITDISTCEQYS 63

Query: 86  EPLLKVMACLPPNQKVILVGHSLGGFNLAVAMDRFPDKIAASVFVAAFVPDTDHKPSYV- 145
           EPL+++M  LP ++KV+LVGHS GG +LA+AMD+FPDKI+ SVFV AF+PDT H PS+V 
Sbjct: 64  EPLMQLMTSLPNDEKVVLVGHSFGGLSLALAMDKFPDKISVSVFVTAFMPDTKHSPSFVE 123

Query: 146 ------------------------------------------------------------ 205
                                                                       
Sbjct: 124 EKFASSMTPEGWMGSELETYGSDNSGLSVFFSTDFMKHRLYQLSPVEDLELGLLLKRPSS 183

[Close]

BLAST of Carg05233 vs. ExPASy Swiss-Prot
Match: O80475 (Methylesterase 8 OS=Arabidopsis thaliana OX=3702 GN=MES8 PE=3 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 2.2e-49
Identity = 100/244 (40.98%), Postives = 137/244 (56.15%), Query Frame = 0


Query: 26  QMEQKHFVFVHGACHGAWSWFKIKPLLEAAGHRVTLLDMAASGIHRKVIQDVHSIVDYSE 85
           +M ++HFV VHG+C GAW W+K+KPLLEA+GHRVT LD+AA GI  + I D+ +   YSE
Sbjct: 22  EMMKQHFVLVHGSCLGAWCWYKVKPLLEASGHRVTALDLAACGIDTRSITDISTCEQYSE 81

Query: 86  PLLKVMACLPPNQKVILVGHSLGGFNLAVAMDRFPDKIAASVFVAAFVPDTDHKPSYVLD 145
           PL+++M  LP ++KV+LVGHS GG  LA+AMD+FPDKI+ SVFV +F+PDT + PS+VL+
Sbjct: 82  PLIQLMTSLPNDEKVVLVGHSYGGLTLAIAMDKFPDKISVSVFVTSFMPDTKNSPSFVLE 141

Query: 146 ------------------------------------------------------------ 205
                                                                       
Sbjct: 142 KFASTMTPEDWMGSELEPYVVFSAEFTKHRILQLSPIEDLELRLLLKRPGSLFLNDLSRM 201

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BLAST of Carg05233 vs. ExPASy Swiss-Prot
Match: Q9SE93 (Polyneuridine-aldehyde esterase OS=Rauvolfia serpentina OX=4060 GN=PNAE PE=1 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 9.2e-48
Identity = 92/251 (36.65%), Postives = 136/251 (54.18%), Query Frame = 0


Query: 28  EQKHFVFVHGACHGAWSWFKIKPLLEAAGHRVTLLDMAASGIHRKVIQDVHSIVDYSEPL 87
           +QKHFV VHG C GAW W+K+KPLLE+AGH+VT +D++A+GI+ + + ++H+  DYSEPL
Sbjct: 9   QQKHFVLVHGGCLGAWIWYKLKPLLESAGHKVTAVDLSAAGINPRRLDEIHTFRDYSEPL 68

Query: 88  LKVMACLPPNQKVILVGHSLGGFNLAVAMDRFPDKIAASVFVAAFVPDTDHKPSYVLDQQ 147
           ++VMA +PP++KV+L+GHS GG +L +AM+ +P+KI+ +VF++A +PD +H  +Y  ++ 
Sbjct: 69  MEVMASIPPDEKVVLLGHSFGGMSLGLAMETYPEKISVAVFMSAMMPDPNHSLTYPFEKY 128

Query: 148 N----------------------------------------------------------- 206
           N                                                           
Sbjct: 129 NEKCPADMMLDSQFSTYGNPENPGMSMILGPQFMALKMFQNCSVEDLELAKMLTRPGSLF 188

[Close]

BLAST of Carg05233 vs. ExPASy TrEMBL
Match: A0A6J1HDY0 (salicylic acid-binding protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111463111 PE=4 SV=1)

HSP 1 Score: 399.1 bits (1024), Expect = 1.8e-107
Identity = 203/279 (72.76%), Postives = 204/279 (73.12%), Query Frame = 0


Query: 1   MTCSHQEFGKSSTFNLNIIFGRDLTQMEQKHFVFVHGACHGAWSWFKIKPLLEAAGHRVT 60
           MTCSHQEFGKSSTFNLNIIFGRDLTQMEQKHFVFVHGACHGAWSWFKIKPLLEAAGHRVT
Sbjct: 1   MTCSHQEFGKSSTFNLNIIFGRDLTQMEQKHFVFVHGACHGAWSWFKIKPLLEAAGHRVT 60

Query: 61  LLDMAASGIHRKVIQDVHSIVDYSEPLLKVMACLPPNQKVILVGHSLGGFNLAVAMDRFP 120
           LLDMAASGIHRKVIQDVHSIVDYSEPLLKVMACLPPNQKVILVGHSLGGFNLAVAM+RFP
Sbjct: 61  LLDMAASGIHRKVIQDVHSIVDYSEPLLKVMACLPPNQKVILVGHSLGGFNLAVAMERFP 120

Query: 121 DKIAASVFVAAFVPDTDHKPSYVLDQ---------------------------------- 180
           DKIAASVFVAAFVPDTDHKPSYVLDQ                                  
Sbjct: 121 DKIAASVFVAAFVPDTDHKPSYVLDQYNEQTPMEAWLDTQFSPYGNEAKPSTSMFFGPDF 180

Query: 181 ---------------------------------------QNYGSVKKVYIVCTEDKGIPE 207
                                                  QNYGSVKKVYIVCTED GIPE
Sbjct: 181 VAKKLYQLSPPEDLALGLSLVRPSSLFMQDLSKANNFSKQNYGSVKKVYIVCTEDIGIPE 240

[Close]

BLAST of Carg05233 vs. ExPASy TrEMBL
Match: A0A6J1KUH6 (salicylic acid-binding protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111497310 PE=4 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 7.7e-90
Identity = 175/253 (69.17%), Postives = 177/253 (69.96%), Query Frame = 0


Query: 27  MEQKHFVFVHGACHGAWSWFKIKPLLEAAGHRVTLLDMAASGIHRKVIQDVHSIVDYSEP 86
           MEQKHFVFVHGACHGAWSWFKIKPLLEAAGHRVTLLDMAASGIHRKVIQDVHSIVDYSEP
Sbjct: 1   MEQKHFVFVHGACHGAWSWFKIKPLLEAAGHRVTLLDMAASGIHRKVIQDVHSIVDYSEP 60

Query: 87  LLKVMACLPPNQKVILVGHSLGGFNLAVAMDRFPDKIAASVFVAAFVPDTDHKPSYVLDQ 146
           LLK MACLPPNQKVILVGHSLGGFNLAVAM+RFPDKIAASVFVAAFVPDT+HKPSYVLDQ
Sbjct: 61  LLKAMACLPPNQKVILVGHSLGGFNLAVAMERFPDKIAASVFVAAFVPDTNHKPSYVLDQ 120

Query: 147 ------------------------------------------------------------ 206
                                                                       
Sbjct: 121 YNEQTPLEAWLDTQFSPYGTEAEPSTSMFFGPNFVAKKLYQLSPPEDLALGLSLLRPSSL 180

[Close]

BLAST of Carg05233 vs. ExPASy TrEMBL
Match: A0A445EXE8 (Uncharacterized protein OS=Arachis hypogaea OX=3818 GN=Ahy_A01g004878 PE=4 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 9.5e-72
Identity = 159/415 (38.31%), Postives = 203/415 (48.92%), Query Frame = 0


Query: 21  GRDLTQMEQKHFVFVHGACHGAWSWFKIKPLLEAAGHRVTLLDMAASGIHRKVIQDVHSI 80
           G +       H++ VHG CHGAW W+KIKPLLE++GH+VTLLD+AASG + K +QDV ++
Sbjct: 2   GSESVTHSSNHYILVHGVCHGAWCWYKIKPLLESSGHKVTLLDLAASGANSKKLQDVETL 61

Query: 81  VDYSEPLLKVMACLPPNQKVILVGHSLGGFNLAVAMDRFPDKIAASVFVAAFVPDTDHKP 140
            +YSEPLL+VM  LPPN+KV+LVGHSLGG N+A+AM++FP K+   VF+ A VPDT HKP
Sbjct: 62  SEYSEPLLEVMESLPPNEKVVLVGHSLGGLNIALAMEKFPTKVQVGVFLTAVVPDTQHKP 121

Query: 141 SYVLD------------------------------------------------------- 200
           SYVL+                                                       
Sbjct: 122 SYVLEKYSESMPATSWLDTEFDKCGNKTSLLFGRIFMSKMLYHLTPLEDLELAMSMLRPG 181

Query: 201 ---------QQN-----YGSVKKVYIVCTEDKGIPEEFQKWMIHNGGIENVMEINGSDHM 260
                    Q+N     YGSV + +IVCT+D G+P EFQ+WMI N GI +VMEING+DHM
Sbjct: 182 SLFIEDLSQQKNFSKVGYGSVPRAFIVCTQDLGVPLEFQRWMIENAGISDVMEINGADHM 241

Query: 261 PMFCKPRQLLHCLLQ----------HGP-------------------------------- 294
            M  KP++L  CLL+          HG                                 
Sbjct: 242 VMLSKPQELCDCLLKIHFILVHGACHGAWCWYKIKPLLESSGHKVTVLDLAASGINLKKL 301

[Close]

BLAST of Carg05233 vs. ExPASy TrEMBL
Match: A0A6J1BTT0 (salicylic acid-binding protein 2-like OS=Momordica charantia OX=3673 GN=LOC111005285 PE=4 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 1.2e-69
Identity = 140/252 (55.56%), Postives = 158/252 (62.70%), Query Frame = 0


Query: 28  EQKHFVFVHGACHGAWSWFKIKPLLEAAGHRVTLLDMAASGIHRKVIQDVHSIVDYSEPL 87
           ++KHFV VHGACHGAW WFKIKPLL AAGHRVT+LDMAASGI  + IQ+V SIV+YSEPL
Sbjct: 5   QKKHFVLVHGACHGAWCWFKIKPLLVAAGHRVTVLDMAASGIDMRPIQEVQSIVEYSEPL 64

Query: 88  LKVMACLPPNQKVILVGHSLGGFNLAVAMDRFPDKIAASVFVAAFVPDTDHKPSYVLDQQ 147
           LK +AC+ PN+KVILVGHSLGG NLAVAM+ FP KIAASVF+ AFVPDTDH PSYVLDQ 
Sbjct: 65  LKALACVAPNEKVILVGHSLGGINLAVAMEHFPHKIAASVFLTAFVPDTDHSPSYVLDQY 124

Query: 148 N----------------------------------------------------------- 207
           N                                                           
Sbjct: 125 NKQSPAEAWLDTQFCPYGNAAEPLTSMFFGPNFMAKKLYQLSPPEDLELGLNLLRPSSLF 184

[Close]

BLAST of Carg05233 vs. ExPASy TrEMBL
Match: A0A0A0KI88 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G490090 PE=4 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 9.8e-69
Identity = 139/251 (55.38%), Postives = 158/251 (62.95%), Query Frame = 0


Query: 28  EQKHFVFVHGACHGAWSWFKIKPLLEAAGHRVTLLDMAASGIHRKVIQDVHSIVDYSEPL 87
           +QKHFV VHGACHGAW WFKIKPLLEAAGHRVTLLDMAASGI ++VIQ+VHS+ +YSEPL
Sbjct: 3   QQKHFVLVHGACHGAWCWFKIKPLLEAAGHRVTLLDMAASGIDKRVIQNVHSMEEYSEPL 62

Query: 88  LKVMACLPPNQKVILVGHSLGGFNLAVAMDRFPDKIAASVFVAAFVPDTDHKPSYVLDQ- 147
           LK +  LPPN+KVILVGHSLGGFNLAVAM+++ +KIA SVF+AAFVPDT HKPSYVL Q 
Sbjct: 63  LKYLDGLPPNEKVILVGHSLGGFNLAVAMEKYSNKIAVSVFLAAFVPDTQHKPSYVLTQY 122

Query: 148 ------------------------------------------------------------ 206
                                                                       
Sbjct: 123 NEKTPKEAWLDTKFAPYGTEAQPSTSMFFGPNFLAKKLYQLSSPQEIVLALTLLRPSSLF 182

[Close]

BLAST of Carg05233 vs. TAIR 10
Match: AT2G23620.1 (methyl esterase 1 )

HSP 1 Score: 209.5 bits (532), Expect = 4.0e-54
Identity = 105/248 (42.34%), Postives = 140/248 (56.45%), Query Frame = 0


Query: 26  QMEQKHFVFVHGACHGAWSWFKIKPLLEAAGHRVTLLDMAASGIH-RKVIQDVHSIVDYS 85
           +  ++HFV VHG+CHGAW W+K+KPLLEA GHRVT +D+AASGI   + I D+ +   YS
Sbjct: 4   EKRKQHFVLVHGSCHGAWCWYKVKPLLEAVGHRVTAVDLAASGIDTTRSITDIPTCEQYS 63

Query: 86  EPLLKVMACLPPNQKVILVGHSLGGFNLAVAMDRFPDKIAASVFVAAFVPDTDHKPSYVL 145
           EPL K++  LP ++KV+LVGHS GG NLA+AM++FP+KI+ +VF+ AF+PDT+H PS+VL
Sbjct: 64  EPLTKLLTSLPNDEKVVLVGHSFGGLNLAIAMEKFPEKISVAVFLTAFMPDTEHSPSFVL 123

Query: 146 DQ---------------------------------------------------------- 201
           D+                                                          
Sbjct: 124 DKFGSNMPQEAWMGTEFEPYGSDNSGLSMFFSPDFMKLGLYQLSPVEDLELGLLLMRPGS 183

[Close]

BLAST of Carg05233 vs. TAIR 10
Match: AT2G23600.1 (acetone-cyanohydrin lyase )

HSP 1 Score: 201.8 bits (512), Expect = 8.3e-52
Identity = 106/253 (41.90%), Postives = 136/253 (53.75%), Query Frame = 0


Query: 26  QMEQKHFVFVHGACHGAWSWFKIKPLLEAAGHRVTLLDMAASGIH-RKVIQDVHSIVDYS 85
           +  ++HFV VHGACHGAW W+K+KPLLEA GHRVT LD+AASGI   + I D+ +   YS
Sbjct: 4   EKRKQHFVLVHGACHGAWCWYKVKPLLEALGHRVTALDLAASGIDTTRSITDISTCEQYS 63

Query: 86  EPLLKVMACLPPNQKVILVGHSLGGFNLAVAMDRFPDKIAASVFVAAFVPDTDHKPSYV- 145
           EPL+++M  LP ++KV+LVGHS GG +LA+AMD+FPDKI+ SVFV AF+PDT H PS+V 
Sbjct: 64  EPLMQLMTSLPNDEKVVLVGHSFGGLSLALAMDKFPDKISVSVFVTAFMPDTKHSPSFVE 123

Query: 146 ------------------------------------------------------------ 205
                                                                       
Sbjct: 124 EKFASSMTPEGWMGSELETYGSDNSGLSVFFSTDFMKHRLYQLSPVEDLELGLLLKRPSS 183

[Close]

BLAST of Carg05233 vs. TAIR 10
Match: AT2G23590.1 (methyl esterase 8 )

HSP 1 Score: 197.6 bits (501), Expect = 1.6e-50
Identity = 100/244 (40.98%), Postives = 137/244 (56.15%), Query Frame = 0


Query: 26  QMEQKHFVFVHGACHGAWSWFKIKPLLEAAGHRVTLLDMAASGIHRKVIQDVHSIVDYSE 85
           +M ++HFV VHG+C GAW W+K+KPLLEA+GHRVT LD+AA GI  + I D+ +   YSE
Sbjct: 22  EMMKQHFVLVHGSCLGAWCWYKVKPLLEASGHRVTALDLAACGIDTRSITDISTCEQYSE 81

Query: 86  PLLKVMACLPPNQKVILVGHSLGGFNLAVAMDRFPDKIAASVFVAAFVPDTDHKPSYVLD 145
           PL+++M  LP ++KV+LVGHS GG  LA+AMD+FPDKI+ SVFV +F+PDT + PS+VL+
Sbjct: 82  PLIQLMTSLPNDEKVVLVGHSYGGLTLAIAMDKFPDKISVSVFVTSFMPDTKNSPSFVLE 141

Query: 146 ------------------------------------------------------------ 205
                                                                       
Sbjct: 142 KFASTMTPEDWMGSELEPYVVFSAEFTKHRILQLSPIEDLELRLLLKRPGSLFLNDLSRM 201

[Close]

BLAST of Carg05233 vs. TAIR 10
Match: AT4G37150.1 (methyl esterase 9 )

HSP 1 Score: 189.1 bits (479), Expect = 5.5e-48
Identity = 93/249 (37.35%), Postives = 131/249 (52.61%), Query Frame = 0


Query: 30  KHFVFVHGACHGAWSWFKIKPLLEAAGHRVTLLDMAASGIHRKVIQDVHSIVDYSEPLLK 89
           KH+V VHG CHGAW W+K+KP+LE +GHRVT+ D+ A G++   ++D+ ++ D+++PLL+
Sbjct: 2   KHYVLVHGGCHGAWCWYKVKPMLEHSGHRVTVFDLTAHGVNMSRVEDIQTLEDFAKPLLE 61

Query: 90  VMACLPPNQKVILVGHSLGGFNLAVAMDRFPDKIAASVFVAAFVPDTDHKPSYVLDQ--- 149
           V+     + KV+LV HSLGG   A+A D FP KI+ +VFV +F+PDT + PSYV ++   
Sbjct: 62  VLESFGSDDKVVLVAHSLGGIPAALAADMFPSKISVAVFVTSFMPDTTNPPSYVFEKFLG 121

Query: 150 ------------------------------------------------------------ 207
                                                                       
Sbjct: 122 SITEEERMDFELGSYGTDDHPLKTAFLGPNYLKNMYLLSPIEDYELAKMLMRVTPAITSN 181

[Close]

BLAST of Carg05233 vs. TAIR 10
Match: AT2G23610.1 (methyl esterase 3 )

HSP 1 Score: 188.3 bits (477), Expect = 9.5e-48
Identity = 101/251 (40.24%), Postives = 135/251 (53.78%), Query Frame = 0


Query: 29  QKHFVFVHGACHGAWSWFKIKPLLEAAGHRVTLLDMAASGIH-RKVIQDVHSIVDYSEPL 88
           ++H V VHGACHGAW W+K+KP LEA+GHRVT +D+AASGI   + I D+ +   YSEPL
Sbjct: 7   KQHVVLVHGACHGAWCWYKVKPQLEASGHRVTAVDLAASGIDMTRSITDISTCEQYSEPL 66

Query: 89  LKVMACLPPNQKVILVGHSLGGFNLAVAMDRFPDKIAASVFVAAFVPDTDHKPSYVLD-- 148
           +++M  LP ++KV+LVGHSLGG +LA+AMD FP KI+ SVFV A +PDT H PS+V D  
Sbjct: 67  MQLMTSLPDDEKVVLVGHSLGGLSLAMAMDMFPTKISVSVFVTAMMPDTKHSPSFVWDKL 126

Query: 149 ------------------------------------------------------------ 207
                                                                       
Sbjct: 127 RKETSREEWLDTVFTSEKPDFPSEFWIFGPEFMAKNLYQLSPVQDLELAKMLVRANPLIK 186

The following BLAST results are available for this feature:

BLAST of Carg05233 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7027073.1	6.5e-192	100.00	Methylesterase 2, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022962696.1	3.8e-107	72.76	salicylic acid-binding protein 2-like [Cucurbita moschata]	[more]
KAG6595055.1	9.1e-93	71.15	Salicylic acid-binding protein 2, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_023003844.1	1.6e-89	69.17	salicylic acid-binding protein 2-like [Cucurbita maxima]	[more]
XP_023518216.1	6.7e-88	67.98	salicylic acid-binding protein 2-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg05233 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6RYA0	3.0e-54	42.86	Salicylic acid-binding protein 2 OS=Nicotiana tabacum OX=4097 GN=SABP2 PE=1 SV=1	[more]
Q8S8S9	5.6e-53	42.34	Methylesterase 1 OS=Arabidopsis thaliana OX=3702 GN=MES1 PE=1 SV=1	[more]
O80476	1.2e-50	41.90	Methylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=MES2 PE=1 SV=1	[more]
O80475	2.2e-49	40.98	Methylesterase 8 OS=Arabidopsis thaliana OX=3702 GN=MES8 PE=3 SV=1	[more]
Q9SE93	9.2e-48	36.65	Polyneuridine-aldehyde esterase OS=Rauvolfia serpentina OX=4060 GN=PNAE PE=1 SV=...	[more]

BLAST of Carg05233 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HDY0	1.8e-107	72.76	salicylic acid-binding protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111463...	[more]
A0A6J1KUH6	7.7e-90	69.17	salicylic acid-binding protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC11149731...	[more]
A0A445EXE8	9.5e-72	38.31	Uncharacterized protein OS=Arachis hypogaea OX=3818 GN=Ahy_A01g004878 PE=4 SV=1	[more]
A0A6J1BTT0	1.2e-69	55.56	salicylic acid-binding protein 2-like OS=Momordica charantia OX=3673 GN=LOC11100...	[more]
A0A0A0KI88	9.8e-69	55.38	AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G490...	[more]

BLAST of Carg05233 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G23620.1	4.0e-54	42.34	methyl esterase 1	[more]
AT2G23600.1	8.3e-52	41.90	acetone-cyanohydrin lyase	[more]
AT2G23590.1	1.6e-50	40.98	methyl esterase 8	[more]
AT4G37150.1	5.5e-48	37.35	methyl esterase 9	[more]
AT2G23610.1	9.5e-48	40.24	methyl esterase 3	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 146..207 e-value: 1.4E-13 score: 53.3
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 210..320 e-value: 9.4E-10 score: 40.7 coord: 24..145 e-value: 2.1E-35 score: 124.7
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 31..206
IPR000073	Alpha/beta hydrolase fold-1	PFAM	PF00561	Abhydrolase_1	coord: 31..150 e-value: 1.4E-15 score: 57.7
None	No IPR available	PANTHER	PTHR10992:SF1027	METHYLESTERASE 1-RELATED	coord: 28..147 coord: 147..207
None	No IPR available	PANTHER	PTHR10992	METHYLESTERASE FAMILY MEMBER	coord: 28..147 coord: 147..207
None	No IPR available	PANTHER	PTHR10992:SF1027	METHYLESTERASE 1-RELATED	coord: 216..296
None	No IPR available	PANTHER	PTHR10992	METHYLESTERASE FAMILY MEMBER	coord: 216..296

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg05233-RA	Carg05233-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032787	monocarboxylic acid metabolic process
molecular_function	GO:0052689	carboxylic ester hydrolase activity