Carg05231 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg05231
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionL-ascorbate oxidase homolog
LocationCarg_Chr07: 3659325 .. 3662122 (+)
RNA-Seq ExpressionCarg05231
SyntenyCarg05231
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAAGCCTTTCCCCCACTCCAGTTGTAATCAATCATCTTCTAGCTGTTACCCACTGACCCCAAAAGGGTTTCAAATTTAACCCTTTTTTCAACCTCGATTTCCTCTGCTTTCCCTCACTCCCTGGTAATCTGAGTGAGTTTTCTTTTCTTTGAACTCTGTCTTCTTATTTTGTTTATGGGGGTTTCTGATGCAATGCCGTTTTGCAGATTCTATGGAGAAACAGGGCTTGCTTCGACTGATTTGTGGAGCTTTGGCTTTGCTGAGCATTTCATTGGTGAATGCTGAAAACCCATATAGGTTTTACACTTGGACAGTTACATATGGGACTATTTCTCCTCTGGGTGTTCCTCAACAGGTTGGTTTACTGCTGTTCATGTTTCAGATCTGGTTTTTACAGTTTATTAATGGTGGGTTTTGTTTGATTTTTAAGGTTATCCTGATAAACGGTCAGTTTCCCGGCCCCAAATTAGAAGTAGTGACCAATGACAATATCATTCTCAACCTTATCAACAAGCTGGATGAGCCTTTCTTGCTTACATGGTGAGTTCCATCAAATCATTCCTTTTTCGCCATTATAATTTCAGTTAATAGCTTCATTCAGACCACGTTACCAAGCCATCTGTTGTTATGTTTTCAAACAATTTCTTAAGTAAATTGGTTGCTTTGGGCTTTAGTTCTGTTCTGTAATGCTTGTTCTGTCTTAAGGAATGGGATCAAACAGAGGAAGAACTCATGGCAAGATGGAGTTCTTGGGACCAACTGTGCCATTCCTCCAAACTCTAATTACACTTACTCCTTCCAAACCAAAGATCAGATTGGATCCTTTGCATACTTCCCTTCTACTCAGTTTCACAAAGCTGCTGGTGGCTTTGGAGCTCTGAATGTCTACCAGAGGCCGCGAATTCCAATTCCTTATCCTGTTCAAGATGGAGACTTTACTCTACTTGTTGGTGATTGGTACAAGACTAGTCACAAGGTATTTTAATATCGAACATGGTCAACTATTAATGACAAGAGTTCTTTGTTAATGTCGGTTTCTTTACTTTTCAGTCCCTGCAAGAATCTCTAGATGCAGGAAGATCTCTTCCCTTACCTGATGGTGTTCTTATAAATGGACAAACTCATAGTACTTTCACTGGCAATCAAGGTTCTTTTCAAAACATATCTATTATGCTTTCCCTTTTATATTAAAGTTTGCTAAGTAACCTAACAACGACTCTTGCAAACATTTCAGGTAAAACTTACATGTTCAGGATCTCAAATGTGGGTTTGTCAACATCTCTTAACTTCAGGATTCAGGGACATAAAATGAAGTTAGTGGAAGTTGAAGGATCTCATACTCTCCAGAACTTATACGACTCTCTCGACGTTCATGTCGGTCAGTCATTATCGGTTCTAGTCACCTTAGATCAGGCTCCAAAGGACTATTATATAGTTGCATCAACACGTTTCTCTAGAACTGTTCTCACAGCAACGGCAGTGTTGCATTATTCAAATTCCAACTCACCTGTTTCTGGACCTTTGCCTGCTGGTCCTACCATTGAAGTAGATTGGTCTGTGAAGCAAGCCAGAACTTTCAGGTATGTCGAGACGATGCAATGCAACGACACGAGGTTTCTTTTTATTGTTACCAACAAGGAGACTGATTTTTTTACCATTCATCTCTGGAAGGTGGAACTTAACAGCAAATGCAGCCAGGCCTAATCCTCAGGGCTCATTCCATTATGGGAAGATTGTCCCAACAAAGACAATTGTTTTGGCAACGTCCGCTCCTTTAATCAATGGAAAACAGCGATATGCCATCAACAGGGTGTCATATGTTAATTCAGACACCCCTCTAAAGCTTGCTGATTACTTCAACATCCCTGGTGTTTTCTCTGTAGATTCCCTCCAGAGCCTTCCCTCTGGTGGCGCTGCCTTCATTGCTACTTCTGTATTGCCAACCGCTCTCCACGATTTCGTTGAAATCGTATTCCAGAACAACGAGCATTCTATGCAATCCTTCCACCTTGATGGTTATGATTTCTGGGTGGTTGGGTAAGTCAAATGTGCTGTGTTCTGCTTCTACAATGTTTCCTTTGCAAATATCTTGATATTTTCCCTTTGTTGGCAATATGATAGATATGGCTATGGACAATGGAGTCAAGCCAGCAGAAACTCCTACAATTTAGTAGATGCTCTTACCAGACACACAGCACAGGTAAACGACTCGGCATCAGTACCATTATGCAGTGACTCTGCTTTGACATTTTGAGCTCTGTAGTTCATTTCAATGACATTTTGCCCTGAGCTTGTTGTGTGGGTTGTTGGTGTTGCAGGTGTATCCCAATTCATGGACAGCCATATTGGTATCATTAGACAATCAAGGGATGTGGAACTTAAGGTCTGCAATCTGGGAAAGACAGTATCTTGGGCAACAGTTCTATCTCAGAGTGTGGACTCAAACTCAGAGTCTTGCCAATGAATACAACATCCCTCCCAACGCTTTGTTATGCGGTAAGGCCATGGGACGACATCTTTGAATCTCGCATTGCCGATCTGTCTGCCTTTAGTCATGAGAGTTTGACTACATTACATGGAGAGAAAAAAAAATGAGAATCATTTACCTCAATTCTTTCAATCGCTTGGAAACTTTTACATGTATTGTTCTTTGAAGTGGCTCAATCAAGTCATGCTTCTAAGTTGTTCATTTCACTATTTATTTTGAGAAAGTAAATGGACTTACAAAAATATTGCTACAACATCTTCCCACCGCTAAACATTAATTATAAGAAAAAGAATGTAAATCAAAGCATTGG

mRNA sequence

TAAGCCTTTCCCCCACTCCAGTTGTAATCAATCATCTTCTAGCTGTTACCCACTGACCCCAAAAGGGTTTCAAATTTAACCCTTTTTTCAACCTCGATTTCCTCTGCTTTCCCTCACTCCCTGATTCTATGGAGAAACAGGGCTTGCTTCGACTGATTTGTGGAGCTTTGGCTTTGCTGAGCATTTCATTGGTGAATGCTGAAAACCCATATAGGTTTTACACTTGGACAGTTACATATGGGACTATTTCTCCTCTGGGTGTTCCTCAACAGGTTATCCTGATAAACGGTCAGTTTCCCGGCCCCAAATTAGAAGTAGTGACCAATGACAATATCATTCTCAACCTTATCAACAAGCTGGATGAGCCTTTCTTGCTTACATGGAATGGGATCAAACAGAGGAAGAACTCATGGCAAGATGGAGTTCTTGGGACCAACTGTGCCATTCCTCCAAACTCTAATTACACTTACTCCTTCCAAACCAAAGATCAGATTGGATCCTTTGCATACTTCCCTTCTACTCAGTTTCACAAAGCTGCTGGTGGCTTTGGAGCTCTGAATGTCTACCAGAGGCCGCGAATTCCAATTCCTTATCCTGTTCAAGATGGAGACTTTACTCTACTTGTTGGTGATTGGTACAAGACTAGTCACAAGTCCCTGCAAGAATCTCTAGATGCAGGAAGATCTCTTCCCTTACCTGATGGTGTTCTTATAAATGGACAAACTCATAGTACTTTCACTGGCAATCAAGGTAAAACTTACATGTTCAGGATCTCAAATGTGGGTTTGTCAACATCTCTTAACTTCAGGATTCAGGGACATAAAATGAAGTTAGTGGAAGTTGAAGGATCTCATACTCTCCAGAACTTATACGACTCTCTCGACGTTCATGTCGGTCAGTCATTATCGGTTCTAGTCACCTTAGATCAGGCTCCAAAGGACTATTATATAGTTGCATCAACACGTTTCTCTAGAACTGTTCTCACAGCAACGGCAGTGTTGCATTATTCAAATTCCAACTCACCTGTTTCTGGACCTTTGCCTGCTGGTCCTACCATTGAAGTAGATTGGTCTGTGAAGCAAGCCAGAACTTTCAGGTGGAACTTAACAGCAAATGCAGCCAGGCCTAATCCTCAGGGCTCATTCCATTATGGGAAGATTGTCCCAACAAAGACAATTGTTTTGGCAACGTCCGCTCCTTTAATCAATGGAAAACAGCGATATGCCATCAACAGGGTGTCATATGTTAATTCAGACACCCCTCTAAAGCTTGCTGATTACTTCAACATCCCTGGTGTTTTCTCTGTAGATTCCCTCCAGAGCCTTCCCTCTGGTGGCGCTGCCTTCATTGCTACTTCTGTATTGCCAACCGCTCTCCACGATTTCGTTGAAATCGTATTCCAGAACAACGAGCATTCTATGCAATCCTTCCACCTTGATGGTTATGATTTCTGGGTGGTTGGATATGGCTATGGACAATGGAGTCAAGCCAGCAGAAACTCCTACAATTTAGTAGATGCTCTTACCAGACACACAGCACAGGTGTATCCCAATTCATGGACAGCCATATTGGTATCATTAGACAATCAAGGGATGTGGAACTTAAGGTCTGCAATCTGGGAAAGACAGTATCTTGGGCAACAGTTCTATCTCAGAGTGTGGACTCAAACTCAGAGTCTTGCCAATGAATACAACATCCCTCCCAACGCTTTGTTATGCGGTAAGGCCATGGGACGACATCTTTGAATCTCGCATTGCCGATCTGTCTGCCTTTAGTCATGAGAGTTTGACTACATTACATGGAGAGAAAAAAAAATGAGAATCATTTACCTCAATTCTTTCAATCGCTTGGAAACTTTTACATGTATTGTTCTTTGAAGTGGCTCAATCAAGTCATGCTTCTAAGTTGTTCATTTCACTATTTATTTTGAGAAAGTAAATGGACTTACAAAAATATTGCTACAACATCTTCCCACCGCTAAACATTAATTATAAGAAAAAGAATGTAAATCAAAGCATTGG

Coding sequence (CDS)

ATGGAGAAACAGGGCTTGCTTCGACTGATTTGTGGAGCTTTGGCTTTGCTGAGCATTTCATTGGTGAATGCTGAAAACCCATATAGGTTTTACACTTGGACAGTTACATATGGGACTATTTCTCCTCTGGGTGTTCCTCAACAGGTTATCCTGATAAACGGTCAGTTTCCCGGCCCCAAATTAGAAGTAGTGACCAATGACAATATCATTCTCAACCTTATCAACAAGCTGGATGAGCCTTTCTTGCTTACATGGAATGGGATCAAACAGAGGAAGAACTCATGGCAAGATGGAGTTCTTGGGACCAACTGTGCCATTCCTCCAAACTCTAATTACACTTACTCCTTCCAAACCAAAGATCAGATTGGATCCTTTGCATACTTCCCTTCTACTCAGTTTCACAAAGCTGCTGGTGGCTTTGGAGCTCTGAATGTCTACCAGAGGCCGCGAATTCCAATTCCTTATCCTGTTCAAGATGGAGACTTTACTCTACTTGTTGGTGATTGGTACAAGACTAGTCACAAGTCCCTGCAAGAATCTCTAGATGCAGGAAGATCTCTTCCCTTACCTGATGGTGTTCTTATAAATGGACAAACTCATAGTACTTTCACTGGCAATCAAGGTAAAACTTACATGTTCAGGATCTCAAATGTGGGTTTGTCAACATCTCTTAACTTCAGGATTCAGGGACATAAAATGAAGTTAGTGGAAGTTGAAGGATCTCATACTCTCCAGAACTTATACGACTCTCTCGACGTTCATGTCGGTCAGTCATTATCGGTTCTAGTCACCTTAGATCAGGCTCCAAAGGACTATTATATAGTTGCATCAACACGTTTCTCTAGAACTGTTCTCACAGCAACGGCAGTGTTGCATTATTCAAATTCCAACTCACCTGTTTCTGGACCTTTGCCTGCTGGTCCTACCATTGAAGTAGATTGGTCTGTGAAGCAAGCCAGAACTTTCAGGTGGAACTTAACAGCAAATGCAGCCAGGCCTAATCCTCAGGGCTCATTCCATTATGGGAAGATTGTCCCAACAAAGACAATTGTTTTGGCAACGTCCGCTCCTTTAATCAATGGAAAACAGCGATATGCCATCAACAGGGTGTCATATGTTAATTCAGACACCCCTCTAAAGCTTGCTGATTACTTCAACATCCCTGGTGTTTTCTCTGTAGATTCCCTCCAGAGCCTTCCCTCTGGTGGCGCTGCCTTCATTGCTACTTCTGTATTGCCAACCGCTCTCCACGATTTCGTTGAAATCGTATTCCAGAACAACGAGCATTCTATGCAATCCTTCCACCTTGATGGTTATGATTTCTGGGTGGTTGGATATGGCTATGGACAATGGAGTCAAGCCAGCAGAAACTCCTACAATTTAGTAGATGCTCTTACCAGACACACAGCACAGGTGTATCCCAATTCATGGACAGCCATATTGGTATCATTAGACAATCAAGGGATGTGGAACTTAAGGTCTGCAATCTGGGAAAGACAGTATCTTGGGCAACAGTTCTATCTCAGAGTGTGGACTCAAACTCAGAGTCTTGCCAATGAATACAACATCCCTCCCAACGCTTTGTTATGCGGTAAGGCCATGGGACGACATCTTTGA

Protein sequence

MEKQGLLRLICGALALLSISLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPKLEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQTKDQIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPVQDGDFTLLVGDWYKTSHKSLQESLDAGRSLPLPDGVLINGQTHSTFTGNQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEGSHTLQNLYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRFSRTVLTATAVLHYSNSNSPVSGPLPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIVPTKTIVLATSAPLINGKQRYAINRVSYVNSDTPLKLADYFNIPGVFSVDSLQSLPSGGAAFIATSVLPTALHDFVEIVFQNNEHSMQSFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTRHTAQVYPNSWTAILVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPPNALLCGKAMGRHL
Homology
BLAST of Carg05231 vs. NCBI nr
Match: KAG7027071.1 (hypothetical protein SDJN02_11080 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1107.4 bits (2863), Expect = 0.0e+00
Identity = 538/538 (100.00%), Postives = 538/538 (100.00%), Query Frame = 0

Query: 1   MEKQGLLRLICGALALLSISLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK 60
           MEKQGLLRLICGALALLSISLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK
Sbjct: 1   MEKQGLLRLICGALALLSISLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK 60

Query: 61  LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQTKD 120
           LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQTKD
Sbjct: 61  LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQTKD 120

Query: 121 QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPVQDGDFTLLVGDWYKTSHKSLQES 180
           QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPVQDGDFTLLVGDWYKTSHKSLQES
Sbjct: 121 QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPVQDGDFTLLVGDWYKTSHKSLQES 180

Query: 181 LDAGRSLPLPDGVLINGQTHSTFTGNQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG 240
           LDAGRSLPLPDGVLINGQTHSTFTGNQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG
Sbjct: 181 LDAGRSLPLPDGVLINGQTHSTFTGNQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG 240

Query: 241 SHTLQNLYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRFSRTVLTATAVLHYSNSNSPV 300
           SHTLQNLYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRFSRTVLTATAVLHYSNSNSPV
Sbjct: 241 SHTLQNLYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRFSRTVLTATAVLHYSNSNSPV 300

Query: 301 SGPLPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIVPTKTIVLATSAPLIN 360
           SGPLPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIVPTKTIVLATSAPLIN
Sbjct: 301 SGPLPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIVPTKTIVLATSAPLIN 360

Query: 361 GKQRYAINRVSYVNSDTPLKLADYFNIPGVFSVDSLQSLPSGGAAFIATSVLPTALHDFV 420
           GKQRYAINRVSYVNSDTPLKLADYFNIPGVFSVDSLQSLPSGGAAFIATSVLPTALHDFV
Sbjct: 361 GKQRYAINRVSYVNSDTPLKLADYFNIPGVFSVDSLQSLPSGGAAFIATSVLPTALHDFV 420

Query: 421 EIVFQNNEHSMQSFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTRHTAQVYPNSWTAI 480
           EIVFQNNEHSMQSFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTRHTAQVYPNSWTAI
Sbjct: 421 EIVFQNNEHSMQSFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTRHTAQVYPNSWTAI 480

Query: 481 LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPPNALLCGKAMGRHL 539
           LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPPNALLCGKAMGRHL
Sbjct: 481 LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPPNALLCGKAMGRHL 538

BLAST of Carg05231 vs. NCBI nr
Match: XP_022963145.1 (L-ascorbate oxidase homolog [Cucurbita moschata])

HSP 1 Score: 1107.0 bits (2862), Expect = 0.0e+00
Identity = 537/538 (99.81%), Postives = 538/538 (100.00%), Query Frame = 0

Query: 1   MEKQGLLRLICGALALLSISLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK 60
           MEKQGLLRLICGALALLSISLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK
Sbjct: 1   MEKQGLLRLICGALALLSISLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK 60

Query: 61  LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQTKD 120
           LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQTKD
Sbjct: 61  LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQTKD 120

Query: 121 QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPVQDGDFTLLVGDWYKTSHKSLQES 180
           QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPVQDGDFTLLVGDWYKTSHKSLQES
Sbjct: 121 QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPVQDGDFTLLVGDWYKTSHKSLQES 180

Query: 181 LDAGRSLPLPDGVLINGQTHSTFTGNQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG 240
           LDAGRSLPLPDGVLINGQTHSTFTGNQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG
Sbjct: 181 LDAGRSLPLPDGVLINGQTHSTFTGNQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG 240

Query: 241 SHTLQNLYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRFSRTVLTATAVLHYSNSNSPV 300
           SHTLQNLYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRFSRTVLTATAVLHYSNSNSPV
Sbjct: 241 SHTLQNLYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRFSRTVLTATAVLHYSNSNSPV 300

Query: 301 SGPLPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIVPTKTIVLATSAPLIN 360
           SGPLPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKI+PTKTIVLATSAPLIN
Sbjct: 301 SGPLPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSAPLIN 360

Query: 361 GKQRYAINRVSYVNSDTPLKLADYFNIPGVFSVDSLQSLPSGGAAFIATSVLPTALHDFV 420
           GKQRYAINRVSYVNSDTPLKLADYFNIPGVFSVDSLQSLPSGGAAFIATSVLPTALHDFV
Sbjct: 361 GKQRYAINRVSYVNSDTPLKLADYFNIPGVFSVDSLQSLPSGGAAFIATSVLPTALHDFV 420

Query: 421 EIVFQNNEHSMQSFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTRHTAQVYPNSWTAI 480
           EIVFQNNEHSMQSFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTRHTAQVYPNSWTAI
Sbjct: 421 EIVFQNNEHSMQSFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTRHTAQVYPNSWTAI 480

Query: 481 LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPPNALLCGKAMGRHL 539
           LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPPNALLCGKAMGRHL
Sbjct: 481 LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPPNALLCGKAMGRHL 538

BLAST of Carg05231 vs. NCBI nr
Match: KAG6595053.1 (hypothetical protein SDJN03_11606, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1105.1 bits (2857), Expect = 0.0e+00
Identity = 536/538 (99.63%), Postives = 538/538 (100.00%), Query Frame = 0

Query: 1   MEKQGLLRLICGALALLSISLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK 60
           MEKQGLLRLICGALALLSISLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK
Sbjct: 42  MEKQGLLRLICGALALLSISLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK 101

Query: 61  LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQTKD 120
           LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQTKD
Sbjct: 102 LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQTKD 161

Query: 121 QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPVQDGDFTLLVGDWYKTSHKSLQES 180
           QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPVQDGDFTLLVGDWYKTSHKSLQES
Sbjct: 162 QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPVQDGDFTLLVGDWYKTSHKSLQES 221

Query: 181 LDAGRSLPLPDGVLINGQTHSTFTGNQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG 240
           LDAGRSLPLPDGVLINGQTHSTFTGNQGKTYMFR+SNVGLSTSLNFRIQGHKMKLVEVEG
Sbjct: 222 LDAGRSLPLPDGVLINGQTHSTFTGNQGKTYMFRVSNVGLSTSLNFRIQGHKMKLVEVEG 281

Query: 241 SHTLQNLYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRFSRTVLTATAVLHYSNSNSPV 300
           SHTLQNLYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRFSRTVLTATAVLHYSNSNSPV
Sbjct: 282 SHTLQNLYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRFSRTVLTATAVLHYSNSNSPV 341

Query: 301 SGPLPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIVPTKTIVLATSAPLIN 360
           SGPLPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIVPTKTIVLATSAPLIN
Sbjct: 342 SGPLPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIVPTKTIVLATSAPLIN 401

Query: 361 GKQRYAINRVSYVNSDTPLKLADYFNIPGVFSVDSLQSLPSGGAAFIATSVLPTALHDFV 420
           GKQRYAINRVSYVNSDTPLKLADYFNIPGVFSVDSLQSLPSGGAAFIATSVLPTALHDFV
Sbjct: 402 GKQRYAINRVSYVNSDTPLKLADYFNIPGVFSVDSLQSLPSGGAAFIATSVLPTALHDFV 461

Query: 421 EIVFQNNEHSMQSFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTRHTAQVYPNSWTAI 480
           EIVFQNNEHSMQSFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTRHTAQVYPNSWTAI
Sbjct: 462 EIVFQNNEHSMQSFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTRHTAQVYPNSWTAI 521

Query: 481 LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPPNALLCGKAMGRHL 539
           LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEY+IPPNALLCGKAMGRHL
Sbjct: 522 LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYDIPPNALLCGKAMGRHL 579

BLAST of Carg05231 vs. NCBI nr
Match: XP_023518323.1 (L-ascorbate oxidase homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1094.7 bits (2830), Expect = 0.0e+00
Identity = 531/538 (98.70%), Postives = 534/538 (99.26%), Query Frame = 0

Query: 1   MEKQGLLRLICGALALLSISLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK 60
           MEKQGLL LICGALALLSISLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK
Sbjct: 1   MEKQGLLPLICGALALLSISLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK 60

Query: 61  LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQTKD 120
           LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQTKD
Sbjct: 61  LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQTKD 120

Query: 121 QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPVQDGDFTLLVGDWYKTSHKSLQES 180
           QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPVQDGDFTLLVGDWYKTSHKSLQES
Sbjct: 121 QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPVQDGDFTLLVGDWYKTSHKSLQES 180

Query: 181 LDAGRSLPLPDGVLINGQTHSTFTGNQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG 240
           LDAGRSLPLPDGVLINGQTHSTFTGNQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG
Sbjct: 181 LDAGRSLPLPDGVLINGQTHSTFTGNQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG 240

Query: 241 SHTLQNLYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRFSRTVLTATAVLHYSNSNSPV 300
           SHTLQNLYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRFSRTVLTATAVLHYSNSNSPV
Sbjct: 241 SHTLQNLYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRFSRTVLTATAVLHYSNSNSPV 300

Query: 301 SGPLPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIVPTKTIVLATSAPLIN 360
           SGPLPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKI+PTKTIVLATSAPLIN
Sbjct: 301 SGPLPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSAPLIN 360

Query: 361 GKQRYAINRVSYVNSDTPLKLADYFNIPGVFSVDSLQSLPSGGAAFIATSVLPTALHDFV 420
           GKQRYAINRVSYVNSDTPLKLADYFNIPGVFSVDSLQSLPS G AFIATSVLPTALHDFV
Sbjct: 361 GKQRYAINRVSYVNSDTPLKLADYFNIPGVFSVDSLQSLPSDGPAFIATSVLPTALHDFV 420

Query: 421 EIVFQNNEHSMQSFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTRHTAQVYPNSWTAI 480
           EIVFQNNEH+MQSFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTRHTAQVYPNSWTAI
Sbjct: 421 EIVFQNNEHTMQSFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTRHTAQVYPNSWTAI 480

Query: 481 LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPPNALLCGKAMGRHL 539
           LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIP NA+LCGKAMGRHL
Sbjct: 481 LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPSNAMLCGKAMGRHL 538

BLAST of Carg05231 vs. NCBI nr
Match: XP_023003431.1 (L-ascorbate oxidase homolog [Cucurbita maxima])

HSP 1 Score: 1088.6 bits (2814), Expect = 0.0e+00
Identity = 529/538 (98.33%), Postives = 532/538 (98.88%), Query Frame = 0

Query: 1   MEKQGLLRLICGALALLSISLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK 60
           MEKQGLLRLIC ALALLSISLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK
Sbjct: 1   MEKQGLLRLICRALALLSISLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK 60

Query: 61  LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQTKD 120
           LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQTKD
Sbjct: 61  LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQTKD 120

Query: 121 QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPVQDGDFTLLVGDWYKTSHKSLQES 180
           QIGSFAYFPSTQF KA GGFGALNVYQRPRIPIPYPVQDGDFTLLVGDWYKTSHKSLQES
Sbjct: 121 QIGSFAYFPSTQFQKAGGGFGALNVYQRPRIPIPYPVQDGDFTLLVGDWYKTSHKSLQES 180

Query: 181 LDAGRSLPLPDGVLINGQTHSTFTGNQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG 240
           LDAGRSLPLPDGVLINGQTHSTFTGNQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG
Sbjct: 181 LDAGRSLPLPDGVLINGQTHSTFTGNQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG 240

Query: 241 SHTLQNLYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRFSRTVLTATAVLHYSNSNSPV 300
           SHTLQNLYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRFSRTVLTATAVLHYSNSNSPV
Sbjct: 241 SHTLQNLYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRFSRTVLTATAVLHYSNSNSPV 300

Query: 301 SGPLPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIVPTKTIVLATSAPLIN 360
           SGPLPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKI+PTKTIVLATSAPLIN
Sbjct: 301 SGPLPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSAPLIN 360

Query: 361 GKQRYAINRVSYVNSDTPLKLADYFNIPGVFSVDSLQSLPSGGAAFIATSVLPTALHDFV 420
           GKQRYAINRVSYVNSDTPLKLADYFNIPGVFSV+SLQSLPS G AFIATSVLPT LHDFV
Sbjct: 361 GKQRYAINRVSYVNSDTPLKLADYFNIPGVFSVNSLQSLPSDGPAFIATSVLPTFLHDFV 420

Query: 421 EIVFQNNEHSMQSFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTRHTAQVYPNSWTAI 480
           EIVFQNNEHSMQSFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTRHTAQVYPNSWTAI
Sbjct: 421 EIVFQNNEHSMQSFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTRHTAQVYPNSWTAI 480

Query: 481 LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPPNALLCGKAMGRHL 539
           LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEY+IPPNALLCGKAMGRHL
Sbjct: 481 LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYSIPPNALLCGKAMGRHL 538

BLAST of Carg05231 vs. ExPASy Swiss-Prot
Match: P29162 (L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 599.0 bits (1543), Expect = 5.4e-170
Identity = 280/535 (52.34%), Postives = 376/535 (70.28%), Query Frame = 0

Query: 14  LALLSISLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPKLEVVTNDNIILNL 73
           L  LS+ ++ AE+PY ++ W VTYGTI+PLGVPQQ ILINGQFPGP++   +N+NI++N+
Sbjct: 12  LLCLSVGVI-AEDPYLYFNWNVTYGTIAPLGVPQQGILINGQFPGPRINCTSNNNIVVNV 71

Query: 74  INKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQTKDQIGSFAYFPSTQF 133
            N LDEPFL TWNG++ RKNSWQDG  GT C I P  N+TY FQ KDQIGS++YFP+T  
Sbjct: 72  FNNLDEPFLFTWNGVQHRKNSWQDGTPGTMCPIMPGQNFTYRFQVKDQIGSYSYFPTTAL 131

Query: 134 HKAAGGFGALNVYQRPRIPIPYPVQDGDFTLLVGDWYKTSHKSLQESLDAGRSLPLPDGV 193
           H+AAGG+GALNV+ R  IP+P+     ++ + VGDWY   HK+L++ LD GR++  PDG+
Sbjct: 132 HRAAGGYGALNVHSRALIPVPFDNPADEYNVFVGDWYNKGHKTLKKILDGGRTIGRPDGI 191

Query: 194 LINGQT-------HSTFTGNQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEGSHTLQN 253
           +ING++          FT   GKTY +R  N+G+ +S+N R QGH MKLVE+EGSHT+QN
Sbjct: 192 IINGKSAKVGEAKEPLFTMEAGKTYRYRFCNLGMRSSVNIRFQGHPMKLVELEGSHTVQN 251

Query: 254 LYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRFSRTVLTATAVLHYSNSNSPVSGPLPA 313
           +YDSLD+HVGQ LSVLVT DQ PKDYY+V S+RF +  L++ A++ Y+N   P S  LP 
Sbjct: 252 IYDSLDLHVGQCLSVLVTADQEPKDYYLVVSSRFLKQALSSVAIIRYANGKGPASPELPT 311

Query: 314 GP---TIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIVPTKTIVLATSAPLINGKQ 373
            P   T  + WS+ Q R+FRWNLTA+AARPNPQGS+HYG+I  T+TI +  S   + GK 
Sbjct: 312 PPPENTEGIAWSMNQFRSFRWNLTASAARPNPQGSYHYGQINITRTIKIFNSMSQVGGKL 371

Query: 374 RYAINRVSYVNSDTPLKLADYFNIPG-VFSVDSL--QSLPSGGAAFIATSVLPTALHDFV 433
           RY +N +S+ N +TPLKL +YF      F  D +  ++        IAT+V      +FV
Sbjct: 372 RYGLNGISHTNGETPLKLVEYFGATNKAFKYDLMADEAPADPSKLTIATNVKNATYRNFV 431

Query: 434 EIVFQNNEHSMQSFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTRHTAQVYPNSWTAI 493
           EI+F+N+E +++++HLDGY F+ V    G+WS   R +YNLVD L+R+  QVYPNSW AI
Sbjct: 432 EIIFENHEKTIRTYHLDGYSFFAVAVEPGRWSPEKRKNYNLVDGLSRNNIQVYPNSWAAI 491

Query: 494 LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPPNALLCGKAMG 536
           +++ DN GMWNLRS +WE+ YLG+Q Y  V + ++SL +EYNIP N  LCG   G
Sbjct: 492 MLTFDNAGMWNLRSEMWEKTYLGEQLYFSVLSPSRSLRDEYNIPDNHPLCGIVKG 545

BLAST of Carg05231 vs. ExPASy Swiss-Prot
Match: Q00624 (L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 2.0e-169
Identity = 292/532 (54.89%), Postives = 371/532 (69.74%), Query Frame = 0

Query: 11  CGALALLSISLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPKLEVVTNDNII 70
           C  LA  +  +V+AE+PY  + W VTYGT SPLGVPQQVILINGQFPGP +   +N+N+I
Sbjct: 10  CLYLAAAATVVVHAEDPYFHHVWNVTYGTASPLGVPQQVILINGQFPGPNINSTSNNNVI 69

Query: 71  LNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQTKDQIGSFAYFPS 130
           +N+ N LDEPFLLTWNGI+ RKN WQDG  GT C I P +NYTY FQ KDQIGS+ Y+P+
Sbjct: 70  INVFNNLDEPFLLTWNGIQHRKNCWQDGTPGTMCPIMPGTNYTYHFQPKDQIGSYFYYPT 129

Query: 131 TQFHKAAGGFGALNVYQRPRIPIPYPVQDGDFTLLVGDWYKTSHKSLQESLDAGRSLPLP 190
           T  H+AAGG+G L V  R  IP+PY   + D+T+L+GDWY  SH  L++ LD GR++  P
Sbjct: 130 TGMHRAAGGYGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTQLKKFLDGGRTIGRP 189

Query: 191 DGVLINGQTHS-------TFTGNQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEGSHT 250
           DG++ING++          FT   GKTY  RI NVG+ TS+NFRIQ HKMKLVE+EGSH 
Sbjct: 190 DGIVINGKSGKGDGSDAPLFTLKPGKTYRVRICNVGVKTSINFRIQNHKMKLVEMEGSHV 249

Query: 251 LQNLYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRFSRTVLTATAVLHYSNSNSPVSGP 310
           LQN YDSLDVHVGQ    +VT +Q PKDYY+VAS+RF +TV+T T +L Y     P S  
Sbjct: 250 LQNDYDSLDVHVGQCFGTIVTANQEPKDYYMVASSRFLKTVITTTGLLRYEGGKGPASSQ 309

Query: 311 LPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIVPTKTIVLATSAPLINGKQ 370
           LPAGP +   WS+ Q R+FRWNLTA+AARPNPQGS+HYGKI  T+TI L  +   ++GK 
Sbjct: 310 LPAGP-VGWAWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNTQGKVDGKL 369

Query: 371 RYAINRVSYVNSDTPLKLADYFNIPG-VFSVDSLQSLPSGGA---AFIATSVLPTALHDF 430
           R+A+N VS+   +TPLKLA+YF I   VF  D++   P+        I  +VL      F
Sbjct: 370 RFALNGVSHTEPETPLKLAEYFGISDKVFKYDTITDDPTPEQIKNIKIEPNVLNITHRTF 429

Query: 431 VEIVFQNNEHSMQSFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTRHTAQVYPNSWTA 490
           VE+VF+N+E S+QS+HLDGY F+ V    G W+   R +YNL+DA++RHT QVYP  W A
Sbjct: 430 VEVVFENHEKSVQSWHLDGYSFFSVAVEPGTWTPEKRKNYNLLDAVSRHTVQVYPKCWAA 489

Query: 491 ILVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPPNALLCG 532
           IL++ DN GMWN+RS   ER+YLGQQ Y  V +  +SL +EYN+P  +L CG
Sbjct: 490 ILLTFDNCGMWNVRSENTERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCG 540

BLAST of Carg05231 vs. ExPASy Swiss-Prot
Match: Q9SU40 (Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=1 SV=1)

HSP 1 Score: 545.0 bits (1403), Expect = 9.2e-154
Identity = 274/537 (51.02%), Postives = 358/537 (66.67%), Query Frame = 0

Query: 17  LSISLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPKLEVVTNDNIILNLINK 76
           ++IS   A +PY FY + V+Y T SPLGVPQQVI ING+FPGP + V TN+N+++N+ NK
Sbjct: 13  VNISFCFAADPYSFYNFEVSYITASPLGVPQQVIAINGKFPGPTINVTTNENLVVNVRNK 72

Query: 77  LDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQTKDQIGSFAYFPSTQFHKA 136
           LDE  LL WNGI+QR+ SWQDGVLGTNC IPP  N+TY FQ KDQIGSF YFPS  F +A
Sbjct: 73  LDEGLLLHWNGIQQRRVSWQDGVLGTNCPIPPKWNWTYEFQVKDQIGSFFYFPSLHFQRA 132

Query: 137 AGGFGALNVYQRPRIPIPYPVQDGDFTLLVGDWYKTSHKSLQESLDAGRSLPLPDGVLIN 196
           +GGFG+  V  R  IP+P+   DGD T+ +GDWY  +H +L+++LD G+ L +PDGVLIN
Sbjct: 133 SGGFGSFVVNPRAIIPVPFSTPDGDITVTIGDWYIRNHTALRKALDDGKDLGMPDGVLIN 192

Query: 197 GQ--------------THSTFTGNQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEGSH 256
           G+                 T T + GKTY  R+SNVG+STSLNFRIQGH + L E EGS+
Sbjct: 193 GKGPYRYNDTLVADGIDFETITVHPGKTYRLRVSNVGISTSLNFRIQGHNLVLAESEGSY 252

Query: 257 TLQNLYDSLDVHVGQSLSVLVTLDQ-APKDYYIVASTR-FSRTV---LTATAVLHYSNSN 316
           T+Q  Y SLD+HVGQS S LVT+DQ A  DYYIVAS R  + T+   +T   +L Y+NS 
Sbjct: 253 TVQQNYTSLDIHVGQSYSFLVTMDQNASSDYYIVASARVVNETIWRRVTGVGILKYTNSK 312

Query: 317 SPVSGPLPAGPTIEVD--WSVKQARTFRWNLTANAARPNPQGSFHYGKIVPTKTIVLATS 376
               G LP GP  E D  +S+ QAR+ RWN++A+ ARPNPQGSF YG I  T   VL   
Sbjct: 313 GKAKGQLPPGPQDEFDKTFSMNQARSIRWNVSASGARPNPQGSFKYGSINVTDVYVLRNM 372

Query: 377 APL-INGKQRYAINRVSYVNSDTPLKLADYFNIPGVFSVDSLQSLPSGGAAFIATSVLPT 436
            P+ I+GK+R  +N +S+ N  TP++LAD   +  V+ +D     P  G A +ATS++  
Sbjct: 373 PPVTISGKRRTTLNGISFKNPSTPIRLADKLKVKDVYKLD-FPKRPLTGPAKVATSIING 432

Query: 437 ALHDFVEIVFQNNEHSMQSFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTRHTAQVYP 496
               F+E+V QNN+  MQS+H+ GY F+VVG  YG+W++ SR +YN  D + R T QVYP
Sbjct: 433 TYRGFMEVVLQNNDTKMQSYHMSGYAFFVVGMDYGEWTENSRGTYNKWDGIARSTIQVYP 492

Query: 497 NSWTAILVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPPNALLCG 532
            +W+AIL+SLDN G WNLR+   +  YLGQ+ Y+RV    ++   E+  P N L CG
Sbjct: 493 GAWSAILISLDNPGAWNLRTENLDSWYLGQETYVRVVNPDENNKTEFGHPDNVLYCG 548

BLAST of Carg05231 vs. ExPASy Swiss-Prot
Match: Q8VXX5 (Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=2 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 1.4e-149
Identity = 272/544 (50.00%), Postives = 356/544 (65.44%), Query Frame = 0

Query: 9   LICGALALLSISLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPKLEVVTNDN 68
           L+C AL L ++S   A +P+  Y + V+Y T SPLGVPQQVI +NGQFPGP L   TN N
Sbjct: 12  LLCFAL-LSAVSF--AADPFVSYDFRVSYLTASPLGVPQQVIAVNGQFPGPLLNATTNYN 71

Query: 69  IILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQTKDQIGSFAYF 128
           +++N+ N LDEP LLTW GI+ R+NSWQDGVLGTNC IPP  N+TY FQ KDQIGSF Y 
Sbjct: 72  VVVNVFNHLDEPLLLTWPGIQMRRNSWQDGVLGTNCPIPPRWNFTYQFQVKDQIGSFFYS 131

Query: 129 PSTQFHKAAGGFGALNVYQRPRIPIPYPVQDGDFTLLVGDWYKTSHKSLQESLDAGRSLP 188
           PS  F +A+GGFG + +  R  IPIP+P  DG+   ++GDWY   HK+L+ +LD+G+ L 
Sbjct: 132 PSLNFQRASGGFGPIVINNRDIIPIPFPQPDGELIFIIGDWYTQDHKALRRALDSGKELG 191

Query: 189 LPDGVLINGQ-------------THSTFTGNQGKTYMFRISNVGLSTSLNFRIQGHKMKL 248
           +PDGVLING+              + TF    GKTY  R+ NVG+STSLNFRIQ H + L
Sbjct: 192 MPDGVLINGKGPYKYNSSVPDGIDYLTFHVEPGKTYRIRVHNVGISTSLNFRIQNHSLLL 251

Query: 249 VEVEGSHTLQNLYDSLDVHVGQSLSVLVTLDQ-APKDYYIVASTRF-SRTV---LTATAV 308
           VE EG +T Q  +   DVHVGQS S LVT+DQ A  DYYIVAS RF + TV   +T  A+
Sbjct: 252 VETEGHYTSQANFTDFDVHVGQSYSFLVTMDQDATSDYYIVASARFVNETVWQRVTGVAI 311

Query: 309 LHYSNSNSPVSGPLPAGPT-IEVDWS-VKQARTFRWNLTANAARPNPQGSFHYGKIVPTK 368
           LHYSNS  PVSGPLP   T +   WS + Q +T R N +A+ ARPNPQGSFHYG+I  T 
Sbjct: 312 LHYSNSKGPVSGPLPVPKTDVSSPWSAMSQPKTIRQNTSASGARPNPQGSFHYGQINITN 371

Query: 369 TIVLATSAP-LINGKQRYAINRVSYVNSDTPLKLADYFNIPGVFSVDSLQSLPSGGAAFI 428
           T +L +  P +ING  R  +N +S+VN  TP++LAD   + G + +D     P      +
Sbjct: 372 TYILRSLPPTIINGALRATLNGISFVNPSTPVRLADRNKVKGAYKLD-FPDRPFNRPLRL 431

Query: 429 ATSVLPTALHDFVEIVFQNNEHSMQSFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTR 488
             S++      F+++VFQNN+  +QSFH+DGY F+VVG  +G WS+  + SYN  DA++R
Sbjct: 432 DRSMINATYKGFIQVVFQNNDTKIQSFHVDGYSFFVVGMDFGIWSEDKKGSYNNWDAISR 491

Query: 489 HTAQVYPNSWTAILVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPPNA 532
            T +VYP  WTA+L+SLDN G+WN+R    +R YLG++ Y+R+    +    E + P N 
Sbjct: 492 STIEVYPGGWTAVLISLDNVGVWNIRVENLDRWYLGEETYMRITNPEEDGKTEMDPPDNV 551

BLAST of Carg05231 vs. ExPASy Swiss-Prot
Match: Q9FHN6 (Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=2 SV=1)

HSP 1 Score: 523.9 bits (1348), Expect = 2.2e-147
Identity = 258/529 (48.77%), Postives = 349/529 (65.97%), Query Frame = 0

Query: 24  AENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPKLEVVTNDNIILNLINKLDEPFLL 83
           A +PY  Y +T++Y T SPLGVPQQVI +NG+FPGP +   TN N+ +N++N LDEP LL
Sbjct: 23  AGDPYVSYDFTLSYITASPLGVPQQVIAVNGKFPGPVINATTNYNVHVNVLNHLDEPLLL 82

Query: 84  TWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQTKDQIGSFAYFPSTQFHKAAGGFGAL 143
           TW G++ R+NSWQDGVLGTNC IPPN N+TY FQ KDQIGS+ Y PS  F +A+GGFGAL
Sbjct: 83  TWPGVQMRRNSWQDGVLGTNCPIPPNWNFTYDFQLKDQIGSYFYSPSLNFQRASGGFGAL 142

Query: 144 NVYQRPRIPIPYPVQDGDFTLLVGDWYKTSHKSLQESLDAGRSLPLPDGVLINGQ----- 203
            +  R  +PIP+   DG+   ++GDWY  +H +L+  LD+G+ L +PDGVLING+     
Sbjct: 143 IINNRDLVPIPFTEPDGEIIFIIGDWYTQNHTALRRILDSGKELGMPDGVLINGKGPFKY 202

Query: 204 --------THSTFTGNQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEGSHTLQNLYDS 263
                    H T   + GKTY  R+ NVG+STSLNFRIQ HK+ L+E EG +T Q  +  
Sbjct: 203 NSSVPDGIEHETVNVDPGKTYRIRVHNVGISTSLNFRIQNHKLLLIETEGRYTSQMNFTD 262

Query: 264 LDVHVGQSLSVLVTLDQ-APKDYYIVASTRF-SRTV---LTATAVLHYSNSNSPVSGPLP 323
            DVHVGQS S LVT+DQ A  DYYIVAS RF + TV   +T   +LHYSNS  P SGPLP
Sbjct: 263 FDVHVGQSYSFLVTMDQNATSDYYIVASARFVNETVWQRVTGVGILHYSNSKGPASGPLP 322

Query: 324 AGPT-IEVDWS-VKQARTFRWNLTANAARPNPQGSFHYGKIVPTKTIVLATSAPL-INGK 383
              T +   WS + Q R  + N +A+ ARPNPQGSFHYG+I  T+T +L +  P  INGK
Sbjct: 323 VSATDVNHPWSAMNQPRAIKQNTSASGARPNPQGSFHYGQINITRTYILRSLPPTKINGK 382

Query: 384 QRYAINRVSYVNSDTPLKLADYFNIPGVFSVDSLQSLPSGGAAFIATSVLPTALHDFVEI 443
            R  +N +S+VN  TP++LAD   + G + +D            +++S++      F+++
Sbjct: 383 LRATLNGISFVNPSTPMRLADDHKVKGDYMLDFPDRPLDEKLPRLSSSIINATYKGFIQV 442

Query: 444 VFQNNEHSMQSFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTRHTAQVYPNSWTAILV 503
           +FQNN+  +QSFH+DGY F+VV   +G WS+   +SYN  DA+ R T +VYP +WTA+L+
Sbjct: 443 IFQNNDTKIQSFHIDGYAFYVVAMDFGIWSEDRNSSYNNWDAVARSTVEVYPGAWTAVLI 502

Query: 504 SLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPPNALLCG 532
           SLDN G+WN+R    +R YLGQ+ Y+R+    ++ + E + P N + CG
Sbjct: 503 SLDNVGVWNIRVENLDRWYLGQETYMRIINPEENGSTEMDPPENVMYCG 551

BLAST of Carg05231 vs. ExPASy TrEMBL
Match: A0A6J1HH59 (L-ascorbate oxidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111463442 PE=3 SV=1)

HSP 1 Score: 1107.0 bits (2862), Expect = 0.0e+00
Identity = 537/538 (99.81%), Postives = 538/538 (100.00%), Query Frame = 0

Query: 1   MEKQGLLRLICGALALLSISLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK 60
           MEKQGLLRLICGALALLSISLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK
Sbjct: 1   MEKQGLLRLICGALALLSISLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK 60

Query: 61  LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQTKD 120
           LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQTKD
Sbjct: 61  LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQTKD 120

Query: 121 QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPVQDGDFTLLVGDWYKTSHKSLQES 180
           QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPVQDGDFTLLVGDWYKTSHKSLQES
Sbjct: 121 QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPVQDGDFTLLVGDWYKTSHKSLQES 180

Query: 181 LDAGRSLPLPDGVLINGQTHSTFTGNQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG 240
           LDAGRSLPLPDGVLINGQTHSTFTGNQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG
Sbjct: 181 LDAGRSLPLPDGVLINGQTHSTFTGNQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG 240

Query: 241 SHTLQNLYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRFSRTVLTATAVLHYSNSNSPV 300
           SHTLQNLYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRFSRTVLTATAVLHYSNSNSPV
Sbjct: 241 SHTLQNLYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRFSRTVLTATAVLHYSNSNSPV 300

Query: 301 SGPLPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIVPTKTIVLATSAPLIN 360
           SGPLPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKI+PTKTIVLATSAPLIN
Sbjct: 301 SGPLPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSAPLIN 360

Query: 361 GKQRYAINRVSYVNSDTPLKLADYFNIPGVFSVDSLQSLPSGGAAFIATSVLPTALHDFV 420
           GKQRYAINRVSYVNSDTPLKLADYFNIPGVFSVDSLQSLPSGGAAFIATSVLPTALHDFV
Sbjct: 361 GKQRYAINRVSYVNSDTPLKLADYFNIPGVFSVDSLQSLPSGGAAFIATSVLPTALHDFV 420

Query: 421 EIVFQNNEHSMQSFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTRHTAQVYPNSWTAI 480
           EIVFQNNEHSMQSFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTRHTAQVYPNSWTAI
Sbjct: 421 EIVFQNNEHSMQSFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTRHTAQVYPNSWTAI 480

Query: 481 LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPPNALLCGKAMGRHL 539
           LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPPNALLCGKAMGRHL
Sbjct: 481 LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPPNALLCGKAMGRHL 538

BLAST of Carg05231 vs. ExPASy TrEMBL
Match: A0A6J1KP89 (L-ascorbate oxidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111497047 PE=3 SV=1)

HSP 1 Score: 1088.6 bits (2814), Expect = 0.0e+00
Identity = 529/538 (98.33%), Postives = 532/538 (98.88%), Query Frame = 0

Query: 1   MEKQGLLRLICGALALLSISLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK 60
           MEKQGLLRLIC ALALLSISLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK
Sbjct: 1   MEKQGLLRLICRALALLSISLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK 60

Query: 61  LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQTKD 120
           LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQTKD
Sbjct: 61  LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQTKD 120

Query: 121 QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPVQDGDFTLLVGDWYKTSHKSLQES 180
           QIGSFAYFPSTQF KA GGFGALNVYQRPRIPIPYPVQDGDFTLLVGDWYKTSHKSLQES
Sbjct: 121 QIGSFAYFPSTQFQKAGGGFGALNVYQRPRIPIPYPVQDGDFTLLVGDWYKTSHKSLQES 180

Query: 181 LDAGRSLPLPDGVLINGQTHSTFTGNQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG 240
           LDAGRSLPLPDGVLINGQTHSTFTGNQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG
Sbjct: 181 LDAGRSLPLPDGVLINGQTHSTFTGNQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG 240

Query: 241 SHTLQNLYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRFSRTVLTATAVLHYSNSNSPV 300
           SHTLQNLYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRFSRTVLTATAVLHYSNSNSPV
Sbjct: 241 SHTLQNLYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRFSRTVLTATAVLHYSNSNSPV 300

Query: 301 SGPLPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIVPTKTIVLATSAPLIN 360
           SGPLPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKI+PTKTIVLATSAPLIN
Sbjct: 301 SGPLPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSAPLIN 360

Query: 361 GKQRYAINRVSYVNSDTPLKLADYFNIPGVFSVDSLQSLPSGGAAFIATSVLPTALHDFV 420
           GKQRYAINRVSYVNSDTPLKLADYFNIPGVFSV+SLQSLPS G AFIATSVLPT LHDFV
Sbjct: 361 GKQRYAINRVSYVNSDTPLKLADYFNIPGVFSVNSLQSLPSDGPAFIATSVLPTFLHDFV 420

Query: 421 EIVFQNNEHSMQSFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTRHTAQVYPNSWTAI 480
           EIVFQNNEHSMQSFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTRHTAQVYPNSWTAI
Sbjct: 421 EIVFQNNEHSMQSFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTRHTAQVYPNSWTAI 480

Query: 481 LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPPNALLCGKAMGRHL 539
           LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEY+IPPNALLCGKAMGRHL
Sbjct: 481 LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYSIPPNALLCGKAMGRHL 538

BLAST of Carg05231 vs. ExPASy TrEMBL
Match: A0A1S3B0P0 (L-ascorbate oxidase homolog OS=Cucumis melo OX=3656 GN=LOC103484753 PE=3 SV=1)

HSP 1 Score: 1058.1 bits (2735), Expect = 1.2e-305
Identity = 510/537 (94.97%), Postives = 524/537 (97.58%), Query Frame = 0

Query: 1   MEKQGLLRLICGALALLSISLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK 60
           MEKQGLLRLI GALALLS+SLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK
Sbjct: 1   MEKQGLLRLIFGALALLSVSLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK 60

Query: 61  LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQTKD 120
           LEVVTNDNIILNL NKLDEP LLTWNGIKQRKNSWQDGV+GTNC IPPNSNYTYSFQTKD
Sbjct: 61  LEVVTNDNIILNLFNKLDEPLLLTWNGIKQRKNSWQDGVVGTNCPIPPNSNYTYSFQTKD 120

Query: 121 QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPVQDGDFTLLVGDWYKTSHKSLQES 180
           QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYP+QDGDFTLL+GDWYKTSHKSLQES
Sbjct: 121 QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPIQDGDFTLLIGDWYKTSHKSLQES 180

Query: 181 LDAGRSLPLPDGVLINGQTHSTFTGNQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG 240
           LD+G SLPLPDGVLINGQTHSTFTG+QGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG
Sbjct: 181 LDSGGSLPLPDGVLINGQTHSTFTGDQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG 240

Query: 241 SHTLQNLYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRFSRTVLTATAVLHYSNSNSPV 300
           SHTLQNLYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRF++TVLTATAVLHYSNSNSPV
Sbjct: 241 SHTLQNLYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRFTKTVLTATAVLHYSNSNSPV 300

Query: 301 SGPLPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIVPTKTIVLATSAPLIN 360
           SGPLPAGPT EVDWSVKQARTFRWNLTANAARPNPQGSFHYGKI+PTKTIVLATSA LIN
Sbjct: 301 SGPLPAGPTTEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSAALIN 360

Query: 361 GKQRYAINRVSYVNSDTPLKLADYFNIPGVFSVDSLQSLPSGGAAFIATSVLPTALHDFV 420
           GKQRYAINRVSYVNSDTPLKLADYFNIPGVFSVDSLQSLPS G AFIATSVLPTALHDFV
Sbjct: 361 GKQRYAINRVSYVNSDTPLKLADYFNIPGVFSVDSLQSLPSDGPAFIATSVLPTALHDFV 420

Query: 421 EIVFQNNEHSMQSFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTRHTAQVYPNSWTAI 480
           EIVFQNNE+++QSFHLDGYDFWVVGYGYGQWSQ SRNSYNLVDALTRHTAQVYPNSWT I
Sbjct: 421 EIVFQNNENTVQSFHLDGYDFWVVGYGYGQWSQDSRNSYNLVDALTRHTAQVYPNSWTTI 480

Query: 481 LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPPNALLCGKAMGRH 538
           LVSLDNQGMWNLRS+IWERQYLGQQFYLRVWT TQSLANEYNIP NALLCGKA+GRH
Sbjct: 481 LVSLDNQGMWNLRSSIWERQYLGQQFYLRVWTPTQSLANEYNIPSNALLCGKAVGRH 537

BLAST of Carg05231 vs. ExPASy TrEMBL
Match: A0A5D3CN89 (L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G005040 PE=3 SV=1)

HSP 1 Score: 1057.7 bits (2734), Expect = 1.6e-305
Identity = 509/537 (94.79%), Postives = 524/537 (97.58%), Query Frame = 0

Query: 1   MEKQGLLRLICGALALLSISLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK 60
           MEKQGLLRLI GALALLS+SLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK
Sbjct: 1   MEKQGLLRLIFGALALLSVSLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK 60

Query: 61  LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQTKD 120
           LEVVTNDNIILNL NKLDEP LLTWNGIKQRKNSWQDGV+GTNC IPPNSNYTYSFQTKD
Sbjct: 61  LEVVTNDNIILNLFNKLDEPLLLTWNGIKQRKNSWQDGVVGTNCPIPPNSNYTYSFQTKD 120

Query: 121 QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPVQDGDFTLLVGDWYKTSHKSLQES 180
           QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYP+QDGDFTLL+GDWYKTSHKSLQES
Sbjct: 121 QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPIQDGDFTLLIGDWYKTSHKSLQES 180

Query: 181 LDAGRSLPLPDGVLINGQTHSTFTGNQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG 240
           LD+G SLPLPDGVL+NGQTHSTFTG+QGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG
Sbjct: 181 LDSGGSLPLPDGVLVNGQTHSTFTGDQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG 240

Query: 241 SHTLQNLYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRFSRTVLTATAVLHYSNSNSPV 300
           SHTLQNLYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRF++TVLTATAVLHYSNSNSPV
Sbjct: 241 SHTLQNLYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRFTKTVLTATAVLHYSNSNSPV 300

Query: 301 SGPLPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIVPTKTIVLATSAPLIN 360
           SGPLPAGPT EVDWSVKQARTFRWNLTANAARPNPQGSFHYGKI+PTKTIVLATSA LIN
Sbjct: 301 SGPLPAGPTTEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSAALIN 360

Query: 361 GKQRYAINRVSYVNSDTPLKLADYFNIPGVFSVDSLQSLPSGGAAFIATSVLPTALHDFV 420
           GKQRYAINRVSYVNSDTPLKLADYFNIPGVFSVDSLQSLPS G AFIATSVLPTALHDFV
Sbjct: 361 GKQRYAINRVSYVNSDTPLKLADYFNIPGVFSVDSLQSLPSDGPAFIATSVLPTALHDFV 420

Query: 421 EIVFQNNEHSMQSFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTRHTAQVYPNSWTAI 480
           EIVFQNNE+++QSFHLDGYDFWVVGYGYGQWSQ SRNSYNLVDALTRHTAQVYPNSWT I
Sbjct: 421 EIVFQNNENTVQSFHLDGYDFWVVGYGYGQWSQDSRNSYNLVDALTRHTAQVYPNSWTTI 480

Query: 481 LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPPNALLCGKAMGRH 538
           LVSLDNQGMWNLRS+IWERQYLGQQFYLRVWT TQSLANEYNIP NALLCGKA+GRH
Sbjct: 481 LVSLDNQGMWNLRSSIWERQYLGQQFYLRVWTPTQSLANEYNIPSNALLCGKAVGRH 537

BLAST of Carg05231 vs. ExPASy TrEMBL
Match: A0A6J1BV74 (L-ascorbate oxidase homolog OS=Momordica charantia OX=3673 GN=LOC111005834 PE=3 SV=1)

HSP 1 Score: 1041.6 bits (2692), Expect = 1.2e-300
Identity = 499/536 (93.10%), Postives = 522/536 (97.39%), Query Frame = 0

Query: 1   MEKQGLLRLICGALALLSISLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK 60
           MEKQG L LI  ALA L++SLVNA++PYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK
Sbjct: 1   MEKQGRLPLIFAALAFLTLSLVNADDPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPK 60

Query: 61  LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQTKD 120
           LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNC IPPNSNYTY+FQTKD
Sbjct: 61  LEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCPIPPNSNYTYAFQTKD 120

Query: 121 QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPVQDGDFTLLVGDWYKTSHKSLQES 180
           QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYP+QDGDFTLL+GDWYK SHKSLQES
Sbjct: 121 QIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPIQDGDFTLLIGDWYKNSHKSLQES 180

Query: 181 LDAGRSLPLPDGVLINGQTHSTFTGNQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEG 240
           LDAGRSLPLPDGVLINGQTHSTF+G+QGKTYMFRISNVGLSTSLNFRIQGHK+KLVEVEG
Sbjct: 181 LDAGRSLPLPDGVLINGQTHSTFSGDQGKTYMFRISNVGLSTSLNFRIQGHKLKLVEVEG 240

Query: 241 SHTLQNLYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRFSRTVLTATAVLHYSNSNSPV 300
           SHTLQNLYDSLDVHVGQSLSVLVTLDQ PKDYYIVASTRF++ VLTATAVLHYSNS+SPV
Sbjct: 241 SHTLQNLYDSLDVHVGQSLSVLVTLDQVPKDYYIVASTRFTKIVLTATAVLHYSNSHSPV 300

Query: 301 SGPLPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIVPTKTIVLATSAPLIN 360
           SGPLPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKI+PTKTIVLATSAPLIN
Sbjct: 301 SGPLPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIIPTKTIVLATSAPLIN 360

Query: 361 GKQRYAINRVSYVNSDTPLKLADYFNIPGVFSVDSLQSLPSGGAAFIATSVLPTALHDFV 420
           GKQRYAINRVS+VNSDTPLKLAD+FNIPGVFSVDSLQSLPS G AF+ATSVLPT+LHDFV
Sbjct: 361 GKQRYAINRVSFVNSDTPLKLADHFNIPGVFSVDSLQSLPSDGPAFVATSVLPTSLHDFV 420

Query: 421 EIVFQNNEHSMQSFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTRHTAQVYPNSWTAI 480
           EIVFQNNE++MQSFHLDGYDFWVVGYGYGQW+QASRNSYNLVDALTRHTAQVYPNSWT I
Sbjct: 421 EIVFQNNENTMQSFHLDGYDFWVVGYGYGQWTQASRNSYNLVDALTRHTAQVYPNSWTTI 480

Query: 481 LVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPPNALLCGKAMGR 537
           LVSLDNQGMWNLRSA+WERQYLGQQFYLRVWTQTQSLANEY IP NALLCGKA+GR
Sbjct: 481 LVSLDNQGMWNLRSAMWERQYLGQQFYLRVWTQTQSLANEYGIPSNALLCGKAVGR 536

BLAST of Carg05231 vs. TAIR 10
Match: AT5G66920.1 (SKU5 similar 17 )

HSP 1 Score: 845.9 bits (2184), Expect = 1.8e-245
Identity = 402/531 (75.71%), Postives = 455/531 (85.69%), Query Frame = 0

Query: 9   LICGALALL-SISLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPKLEVVTND 68
           L+ GAL LL S+ +V  E+PY+FYTWTVTYG ISPLGVPQQVILINGQFPGPKLEVVTND
Sbjct: 16  LLLGALTLLSSLVIVKGESPYKFYTWTVTYGIISPLGVPQQVILINGQFPGPKLEVVTND 75

Query: 69  NIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQTKDQIGSFAY 128
           NIILNLINKLD+PFLLTWNGIKQRKNSWQDGVLGTNC I PNSN+TY FQTKDQIG+F Y
Sbjct: 76  NIILNLINKLDQPFLLTWNGIKQRKNSWQDGVLGTNCPIQPNSNFTYKFQTKDQIGTFNY 135

Query: 129 FPSTQFHKAAGGFGALNVYQRPRIPIPYPVQDGDFTLLVGDWYKTSHKSLQESLDAGRSL 188
           FPST FHKAAGGFGA+NVY RP IPIPYP+   DFTLLVGDW+KT+HK+LQ+ LD+G  L
Sbjct: 136 FPSTAFHKAAGGFGAINVYARPGIPIPYPLPTADFTLLVGDWFKTNHKTLQQRLDSGGVL 195

Query: 189 PLPDGVLINGQTHSTFTGNQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEGSHTLQNL 248
           P PDG+LINGQT STF+G+QGKTYM RISNVGLS++ NFRIQGH MK+VEVEGSH +Q  
Sbjct: 196 PFPDGMLINGQTQSTFSGDQGKTYMLRISNVGLSSTFNFRIQGHTMKVVEVEGSHVIQTD 255

Query: 249 YDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRFSRTVLTATAVLHYSNSNSPVSGPLPAG 308
           YDSLD+HVGQSL+VLVTL+Q+PKDYYIVASTRF R+ L+   +L YSNS  P SG  PA 
Sbjct: 256 YDSLDIHVGQSLAVLVTLNQSPKDYYIVASTRFIRSKLSVMGLLRYSNSRVPASGDPPAL 315

Query: 309 PTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIVPTKTIVLATSAPLINGKQRYAI 368
           P  E+ WS++QARTFRWNLTANAARPNPQGSFHYG I PTKT V + SAPLINGKQRYA+
Sbjct: 316 PPGELVWSMRQARTFRWNLTANAARPNPQGSFHYGMISPTKTFVFSNSAPLINGKQRYAV 375

Query: 369 NRVSYVNSDTPLKLADYFNIPGVFSVDSLQSLPSGGAAFIATSVLPTALHDFVEIVFQNN 428
           N VSYV S+TPLKLAD+F I GVFS +++QS+PS     +ATSV+ T+ HDF+EIVFQNN
Sbjct: 376 NGVSYVKSETPLKLADHFGISGVFSTNAIQSVPSNSPPTVATSVVQTSHHDFLEIVFQNN 435

Query: 429 EHSMQSFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTRHTAQVYPNSWTAILVSLDNQ 488
           E SMQS+HLDGYDFWVVG+G GQW+ A R+ +NLVDALTRHT QVYP SWT ILVSLDNQ
Sbjct: 436 EKSMQSWHLDGYDFWVVGFGSGQWTPAKRSLHNLVDALTRHTTQVYPESWTTILVSLDNQ 495

Query: 489 GMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPPNALLCGKAMGRHL 539
           GMWN+RSAIWERQY GQQFYL+VW   QSLANEYN P N  LCGKA+GRH+
Sbjct: 496 GMWNMRSAIWERQYSGQQFYLKVWNSVQSLANEYNPPDNLQLCGKAVGRHV 546

BLAST of Carg05231 vs. TAIR 10
Match: AT2G23630.1 (SKU5 similar 16 )

HSP 1 Score: 719.9 bits (1857), Expect = 1.5e-207
Identity = 343/522 (65.71%), Postives = 405/522 (77.59%), Query Frame = 0

Query: 13  ALALLSISLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPKLEVVTNDNIILN 72
           A    S+ ++NAE+PY F+TWTVTYGT SPLGVPQQVILINGQFPGP +E VTN+NI++N
Sbjct: 14  AYCFSSVFVINAEDPYLFFTWTVTYGTRSPLGVPQQVILINGQFPGPPIEGVTNNNIVVN 73

Query: 73  LINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQTKDQIGSFAYFPSTQ 132
           +INKLDEPFL+TWNGIKQRK SWQDGVLGTNC I P S++TY FQ KDQIG++AYF ST 
Sbjct: 74  VINKLDEPFLITWNGIKQRKMSWQDGVLGTNCPIQPKSSWTYHFQLKDQIGTYAYFASTS 133

Query: 133 FHKAAGGFGALNVYQRPRIPIPYPVQDGDFTLLVGDWYKTSHKSLQESLDAGRSLPLPDG 192
            H+A+G FGALNV QR  I +PYP  D DFTLLV DWYK  HK LQ  LD+ R+LP PDG
Sbjct: 134 MHRASGAFGALNVNQRSVIFVPYPKPDADFTLLVSDWYKMGHKELQRRLDSSRALPPPDG 193

Query: 193 VLINGQTHS-TFTGNQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEGSHTLQNLYDSL 252
           +LING +    FTG  GK Y FRISNVG+STS+NFRIQGH M LVEVEGSHTLQ +Y+SL
Sbjct: 194 LLINGASKGLVFTGQHGKIYRFRISNVGISTSINFRIQGHMMTLVEVEGSHTLQEVYESL 253

Query: 253 DVHVGQSLSVLVTLDQAPKDYYIVASTRFSRTVLTATAVLHYSNSNSPVSGPLPAGPTIE 312
           D+HVGQS++VLVTL    KDY+IVASTRF++ +LT T +L Y  S    S PLP GPT  
Sbjct: 254 DIHVGQSVTVLVTLKAPVKDYFIVASTRFTKPILTTTGILSYQGSKIRPSHPLPIGPTYH 313

Query: 313 VDWSVKQARTFRWNLTANAARPNPQGSFHYGKIVPTKTIVLATSAPLINGKQRYAINRVS 372
           + WS+KQART R NLTANAARPNPQGSFHYG I   +T VLA    +INGK RY +NRVS
Sbjct: 314 IHWSMKQARTIRLNLTANAARPNPQGSFHYGTIPINRTFVLANGRAMINGKLRYTVNRVS 373

Query: 373 YVNSDTPLKLADYFNIPGVFSVDSLQSLPSGGAAFIATSVLPTALHDFVEIVFQNNEHSM 432
           YVN  TPLKLAD+FNIPGVF+  ++ ++P+ G + + TSV   ALH++VE VFQNNE S+
Sbjct: 374 YVNPATPLKLADWFNIPGVFNFKTIMNIPTPGPSILGTSVFDVALHEYVEFVFQNNEGSI 433

Query: 433 QSFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTRHTAQVYPNSWTAILVSLDNQGMWN 492
           QS+HLDG   +VVGYG G W+ A R  YNLVDA++RHT QVYP SWT+ILVSLDN+GMWN
Sbjct: 434 QSWHLDGTSAYVVGYGSGTWNMAKRRGYNLVDAVSRHTFQVYPMSWTSILVSLDNKGMWN 493

Query: 493 LRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPPNALLCGKA 534
           LRS IW R+YLGQ+ Y+RVW   +SL  E   P N L CGKA
Sbjct: 494 LRSQIWSRRYLGQELYVRVWNNEKSLYTESEPPVNVLFCGKA 535

BLAST of Carg05231 vs. TAIR 10
Match: AT4G37160.1 (SKU5 similar 15 )

HSP 1 Score: 719.5 bits (1856), Expect = 1.9e-207
Identity = 347/537 (64.62%), Postives = 413/537 (76.91%), Query Frame = 0

Query: 1   MEKQGLL--RLICGALALLSISLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPG 60
           M++  LL  +L  GAL  L   LVNAE+PY FYTWTVTYGT SPLGVPQQVILINGQFPG
Sbjct: 1   MKQTNLLVCKLFIGALFWLGSVLVNAEDPYMFYTWTVTYGTRSPLGVPQQVILINGQFPG 60

Query: 61  PKLEVVTNDNIILNLINKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQT 120
           P +E VTN+NI++NLINKLDEPFL+TWNG+KQR+ SWQDGVLGTNC I PNSN+TY FQ 
Sbjct: 61  PAIEAVTNNNIVVNLINKLDEPFLITWNGVKQRRTSWQDGVLGTNCPIQPNSNWTYQFQL 120

Query: 121 KDQIGSFAYFPSTQFHKAAGGFGALNVYQRPRIPIPYPVQDGDFTLLVGDWYKT-SHKSL 180
           KDQIG++ YF ST  H+A+G FGALN+ QR  I  PYP  DGDFTLLV DW+   +HK L
Sbjct: 121 KDQIGTYTYFASTSLHRASGAFGALNINQRSVITTPYPTPDGDFTLLVSDWFSNMTHKDL 180

Query: 181 QESLDAGRSLPLPDGVLINGQTHS-TFTGNQGKTYMFRISNVGLSTSLNFRIQGHKMKLV 240
           ++SLDAG +LPLPD +LING +    FTG QGKTY FR+SNVG++TS+NFRIQ H M L+
Sbjct: 181 RKSLDAGSALPLPDALLINGVSKGLIFTGQQGKTYKFRVSNVGIATSINFRIQNHTMSLI 240

Query: 241 EVEGSHTLQNLYDSLDVHVGQSLSVLVTLDQAPKDYYIVASTRFSRTVLTATAVLHYSNS 300
           EVEG+HTLQ  Y+SLDVHVGQS++VLVTL  + +DY+IVASTRF++ VLT TA L Y  S
Sbjct: 241 EVEGAHTLQESYESLDVHVGQSMTVLVTLKASVRDYFIVASTRFTKPVLTTTASLRYQGS 300

Query: 301 NSPVSGPLPAGPTIEVDWSVKQARTFRWNLTANAARPNPQGSFHYGKIVPTKTIVLATSA 360
            +   GPLP GPT  + WS+KQART R NLTANAARPNPQGSFHYG I   +T+VLA +A
Sbjct: 301 KNAAYGPLPIGPTYHIHWSMKQARTIRMNLTANAARPNPQGSFHYGTIPINRTLVLANAA 360

Query: 361 PLINGKQRYAINRVSYVNSDTPLKLADYFNIPGVFSVDSLQSLPSGGAAFIATSVLPTAL 420
            LI GK RY +NR+SY+N  TPLKLAD++NI GVF   ++ S P+ G A I TSV+   L
Sbjct: 361 TLIYGKLRYTVNRISYINPTTPLKLADWYNISGVFDFKTIISTPTTGPAHIGTSVIDVEL 420

Query: 421 HDFVEIVFQNNEHSMQSFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTRHTAQVYPNS 480
           H+FVEIVFQN+E S+QS+H+DG   + VGYG G W+   R  YNLVDA+ RHT QVYP S
Sbjct: 421 HEFVEIVFQNDERSIQSWHMDGTSAYAVGYGSGTWNVTMRKRYNLVDAVPRHTFQVYPLS 480

Query: 481 WTAILVSLDNQGMWNLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPPNALLCGKA 534
           WT ILVSLDN+GMWNLRS IW R+YLGQ+ Y+RVW   +SL  E   P N L CGKA
Sbjct: 481 WTTILVSLDNKGMWNLRSQIWSRRYLGQELYVRVWNDEKSLYTEAEPPLNVLYCGKA 537

BLAST of Carg05231 vs. TAIR 10
Match: AT4G22010.1 (SKU5 similar 4 )

HSP 1 Score: 710.3 bits (1832), Expect = 1.2e-204
Identity = 336/527 (63.76%), Postives = 414/527 (78.56%), Query Frame = 0

Query: 14  LALLSISLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPKLEVVTNDNIILNL 73
           L L+ I+ V  +NPYRF+TW +TYG I PLGV QQ ILINGQFPGP ++ +TNDNII+++
Sbjct: 11  LLLVLINGVLGDNPYRFFTWKITYGDIYPLGVKQQGILINGQFPGPHIDAITNDNIIISV 70

Query: 74  INKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQTKDQIGSFAYFPSTQF 133
            N L EPFL++WNG++QRKNSWQDGV+GT C IPP  N+TY  Q KDQIGSF YFPS  F
Sbjct: 71  FNYLKEPFLISWNGVQQRKNSWQDGVVGTTCPIPPGKNFTYVIQVKDQIGSFYYFPSLAF 130

Query: 134 HKAAGGFGALNVYQRPRIPIPYPVQDGDFTLLVGDWYKTSHKSLQESLDAGRSLPLPDGV 193
           HKAAG FGA+ V+ RPRIP+P+   DGDF LL GDWYKT+H  L+  L+AGR+LP PDGV
Sbjct: 131 HKAAGAFGAIRVWSRPRIPVPFSPPDGDFWLLAGDWYKTNHYVLRRLLEAGRNLPNPDGV 190

Query: 194 LINGQ--THSTFTGNQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEGSHTLQNLYDSL 253
           LING+    +TFT   GKTY FRISNVG++TSLNFRIQGH MKLVEVEGSHT+QN+Y SL
Sbjct: 191 LINGRGWGGNTFTVQPGKTYRFRISNVGVATSLNFRIQGHTMKLVEVEGSHTVQNIYTSL 250

Query: 254 DVHVGQSLSVLVTLDQAPKDYYIVASTRFSRTVLTATAVLHYSNSNSPVSGPLPAGPTIE 313
           D+H+GQS SVLVT +QAP+DYYIV S+RF+R VLT T++LHYSNS   VSGP+P GPT++
Sbjct: 251 DIHLGQSYSVLVTANQAPQDYYIVISSRFTRKVLTTTSILHYSNSRKGVSGPVPNGPTLD 310

Query: 314 VDWSVKQARTFRWNLTANAARPNPQGSFHYGKIVPTKTIVLATSAPLINGKQRYAINRVS 373
           +  S+ QART R NLTA+  RPNPQGS+HYG I P +TI+LA SAP INGKQRYA+N  S
Sbjct: 311 IASSLYQARTIRRNLTASGPRPNPQGSYHYGLIKPGRTIILANSAPWINGKQRYAVNGAS 370

Query: 374 YVNSDTPLKLADYFNIPGVFSVDSLQSLPSGG-AAFIATSVLPTALHDFVEIVFQNNEHS 433
           +V  DTPLKLADYF IPGVF++ S+ + PSGG   ++ +SV+     +F+E+VFQN E+S
Sbjct: 371 FVAPDTPLKLADYFKIPGVFNLGSIPTSPSGGNGGYLQSSVMAANFREFIEVVFQNWENS 430

Query: 434 MQSFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTRHTAQVYPNSWTAILVSLDNQGMW 493
           +QS+H+ GY F+VVG   GQW+  SR  YNL DA++R T QVYP +WTAI ++LDN GMW
Sbjct: 431 VQSWHVSGYSFFVVGMDGGQWTPGSRAKYNLRDAVSRSTVQVYPRAWTAIYIALDNVGMW 490

Query: 494 NLRSAIWERQYLGQQFYLRVWTQTQSLANEYNIPPNALLCGKAMGRH 538
           N+RS  W RQYLGQQFYLRV+T + S  +EY  P NAL+CG+A GRH
Sbjct: 491 NIRSENWARQYLGQQFYLRVYTSSTSYRDEYPPPKNALMCGRAKGRH 537

BLAST of Carg05231 vs. TAIR 10
Match: AT1G76160.1 (SKU5 similar 5 )

HSP 1 Score: 696.4 bits (1796), Expect = 1.8e-200
Identity = 329/524 (62.79%), Postives = 397/524 (75.76%), Query Frame = 0

Query: 14  LALLSISLVNAENPYRFYTWTVTYGTISPLGVPQQVILINGQFPGPKLEVVTNDNIILNL 73
           + L  +  V AE+PYRF+ W +TYG I PLGV QQ ILING FPGP +  VTNDN+I+N+
Sbjct: 13  IGLSLLFAVTAEDPYRFFEWNITYGDIYPLGVRQQGILINGAFPGPDIHSVTNDNLIINV 72

Query: 74  INKLDEPFLLTWNGIKQRKNSWQDGVLGTNCAIPPNSNYTYSFQTKDQIGSFAYFPSTQF 133
            N LDEPFLL+WNGI+QR+NS+ DGV GT C IPP  NYTY  Q KDQIGSF YFPS  F
Sbjct: 73  YNSLDEPFLLSWNGIQQRRNSFVDGVYGTTCPIPPGKNYTYILQMKDQIGSFYYFPSLGF 132

Query: 134 HKAAGGFGALNVYQRPRIPIPYPVQDGDFTLLVGDWYKTSHKSLQESLDAGRSLPLPDGV 193
           HKAAGGFG + +  RPRIP+P+P   GD T+L+GDWYK +H  L+  LD G+ LPLPDG+
Sbjct: 133 HKAAGGFGGIRILSRPRIPVPFPDPAGDTTVLIGDWYKANHTDLRAQLDNGKKLPLPDGI 192

Query: 194 LINGQTH-STFTGNQGKTYMFRISNVGLSTSLNFRIQGHKMKLVEVEGSHTLQNLYDSLD 253
           LING++  +T    QGKTY FRISNVGL  SLNFRIQ HKMK+VEVEG+HTLQ  + SLD
Sbjct: 193 LINGRSSGATLNVEQGKTYRFRISNVGLQDSLNFRIQDHKMKVVEVEGTHTLQTTFSSLD 252

Query: 254 VHVGQSLSVLVTLDQAPKDYYIVASTRFSRTVLTATAVLHYSNSNSPVSGPLPAGPTIEV 313
           VHVGQS SVLVT DQ P+DYY+V S+RF+  VLT T +  YSNS   VSGP+P GPTI++
Sbjct: 253 VHVGQSYSVLVTADQTPRDYYVVVSSRFTSNVLTTTGIFRYSNSAGGVSGPIPGGPTIQI 312

Query: 314 DWSVKQARTFRWNLTANAARPNPQGSFHYGKIVPTKTIVLATSAPLINGKQRYAINRVSY 373
           DWS+ QAR  R NL+A+  RPNPQGS+HYG I  T+TI LA+SA  ++GKQRYA+N VS+
Sbjct: 313 DWSLNQARAIRTNLSASGPRPNPQGSYHYGMINTTRTIRLASSAGQVDGKQRYAVNSVSF 372

Query: 374 VNSDTPLKLADYFNIPGVFSVDSLQSLPSGGAAFIATSVLPTALHDFVEIVFQNNEHSMQ 433
             +DTPLK+ADYF I GV+   S+Q  P+GG  ++ TSV+      FVEI+F+N+E  +Q
Sbjct: 373 KPADTPLKIADYFKIDGVYRSGSIQYQPTGGGIYLDTSVMQVDYRTFVEIIFENSEDIVQ 432

Query: 434 SFHLDGYDFWVVGYGYGQWSQASRNSYNLVDALTRHTAQVYPNSWTAILVSLDNQGMWNL 493
           S+HLDGY FWVVG   GQWS  SRN YNL DA+ R T QVYP+SWTAIL++LDN GMWNL
Sbjct: 433 SWHLDGYSFWVVGMDGGQWSPDSRNEYNLRDAVARCTVQVYPSSWTAILIALDNVGMWNL 492

Query: 494 RSAIWERQYLGQQFYLRVWTQTQSLANEYNIPPNALLCGKAMGR 537
           RS  W RQYLGQQ YLRV+T + SL +EY IP NALLCG+A GR
Sbjct: 493 RSEFWARQYLGQQLYLRVYTPSTSLRDEYPIPKNALLCGRASGR 536

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7027071.10.0e+00100.00hypothetical protein SDJN02_11080 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022963145.10.0e+0099.81L-ascorbate oxidase homolog [Cucurbita moschata][more]
KAG6595053.10.0e+0099.63hypothetical protein SDJN03_11606, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023518323.10.0e+0098.70L-ascorbate oxidase homolog [Cucurbita pepo subsp. pepo][more]
XP_023003431.10.0e+0098.33L-ascorbate oxidase homolog [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
P291625.4e-17052.34L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q006242.0e-16954.89L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1[more]
Q9SU409.2e-15451.02Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=... [more]
Q8VXX51.4e-14950.00Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=... [more]
Q9FHN62.2e-14748.77Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1HH590.0e+0099.81L-ascorbate oxidase homolog OS=Cucurbita moschata OX=3662 GN=LOC111463442 PE=3 S... [more]
A0A6J1KP890.0e+0098.33L-ascorbate oxidase homolog OS=Cucurbita maxima OX=3661 GN=LOC111497047 PE=3 SV=... [more]
A0A1S3B0P01.2e-30594.97L-ascorbate oxidase homolog OS=Cucumis melo OX=3656 GN=LOC103484753 PE=3 SV=1[more]
A0A5D3CN891.6e-30594.79L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A6J1BV741.2e-30093.10L-ascorbate oxidase homolog OS=Momordica charantia OX=3673 GN=LOC111005834 PE=3 ... [more]
Match NameE-valueIdentityDescription
AT5G66920.11.8e-24575.71SKU5 similar 17 [more]
AT2G23630.11.5e-20765.71SKU5 similar 16 [more]
AT4G37160.11.9e-20764.62SKU5 similar 15 [more]
AT4G22010.11.2e-20463.76SKU5 similar 4 [more]
AT1G76160.11.8e-20062.79SKU5 similar 5 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001117Multicopper oxidase, type 1PFAMPF00394Cu-oxidasecoord: 161..295
e-value: 5.0E-40
score: 137.2
IPR008972CupredoxinGENE3D2.60.40.420coord: 19..147
e-value: 1.1E-33
score: 118.3
IPR008972CupredoxinGENE3D2.60.40.420coord: 343..536
e-value: 5.7E-38
score: 132.7
IPR008972CupredoxinGENE3D2.60.40.420coord: 158..332
e-value: 6.3E-37
score: 129.6
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 159..333
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 346..532
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 25..175
IPR011706Multicopper oxidase, C-terminalPFAMPF07731Cu-oxidase_2coord: 397..514
e-value: 1.4E-27
score: 96.1
IPR011707Multicopper oxidase, N-termianlPFAMPF07732Cu-oxidase_3coord: 35..148
e-value: 1.7E-38
score: 131.3
NoneNo IPR availablePANTHERPTHR11709:SF280MULTI-COPPER OXIDASE-LIKE PROTEINcoord: 12..534
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 12..534
IPR034273Ascorbate oxidase homologue, first cupredoxin domainCDDcd13846CuRO_1_AAO_like_1coord: 30..145
e-value: 4.2307E-74
score: 228.831

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg05231-RACarg05231-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005507 copper ion binding
molecular_function GO:0008447 L-ascorbate oxidase activity
molecular_function GO:0016491 oxidoreductase activity