Carg05169 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg05169
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Poly(A)-specific ribonuclease
Location	Carg_Chr07: 3288099 .. 3288965 (-)
RNA-Seq Expression	Carg05169
Synteny	Carg05169

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTAGAAGAAATCGAGCACGTCCTAGAAGAAGAACACCTTCGATCCAAGAAGTTTGGGCTTCCAATTTCGATCATGAAATCCTCCGCCTCGAACGCTGCCTTCAAGCTAACCATATTCTATCCATCGACACCGAATTTCCTGGGTGCATTAGGAGCACCCCATGGGGCTCGATCGATGAACGCGTCTACGAAGATCTCCGATTCAATGTGAACCAAACGAAGTTAATTCAACTGGGTTTAACAGCTTCTGATGAATCTGGGCAAGTCGTCGGCTCATGGGAGTTCAACTTCTCCGATTTCGACCATCAAATCGACACTCAAAATCCTTCATCGATTTCGTTTCTGGAACAGAACGGGCTCGATTTTGCGAAGCTCAAGAAACAGGGGATCCCCTTAAGCCTATTCACTCTAAACTTCTCGCGTATCATACGAAGAAGCCGTGTTCGTAAATGGGTCACATTCCATGGATTATACGATATTGGGTATCTTGTGAAGGCGATGGGGGTAGCGGAGGAGTTGCCGGAATCAATGGAGGAATTTGCAGAGATGGTGGCGCGTGGAGTTGGGGTTGTGAGGGATTTGAAGGAGGTGGCTCGGTGGTGCGAAGGGCTGGAACAGGGGAGGGTTGGGCTTGAGAGACTTGGAGAATTGCTGGGCGAGAAGCGGTTTGGGATGAAGCACCACGCTGGTTCGGATAGCTTGTTGACGGCTTTTGTGCATGCGAAGATGGCGCGGTGGTTCCGTTTGGAATCAGGAGCGTATGACGGGCATTTGTATGGGCTTCAGAACAAGCTCGAACACATGAAGGTGAATCAGACGATCATGTTTGGGCAGATTTCTTGTTCGTTGTGGGATTATGAATGA

mRNA sequence

ATGGGTAGAAGAAATCGAGCACGTCCTAGAAGAAGAACACCTTCGATCCAAGAAGTTTGGGCTTCCAATTTCGATCATGAAATCCTCCGCCTCGAACGCTGCCTTCAAGCTAACCATATTCTATCCATCGACACCGAATTTCCTGGGTGCATTAGGAGCACCCCATGGGGCTCGATCGATGAACGCGTCTACGAAGATCTCCGATTCAATGTGAACCAAACGAAGTTAATTCAACTGGGTTTAACAGCTTCTGATGAATCTGGGCAAGTCGTCGGCTCATGGGAGTTCAACTTCTCCGATTTCGACCATCAAATCGACACTCAAAATCCTTCATCGATTTCGTTTCTGGAACAGAACGGGCTCGATTTTGCGAAGCTCAAGAAACAGGGGATCCCCTTAAGCCTATTCACTCTAAACTTCTCGCGTATCATACGAAGAAGCCGTGTTCGTAAATGGGTCACATTCCATGGATTATACGATATTGGGTATCTTGTGAAGGCGATGGGGGTAGCGGAGGAGTTGCCGGAATCAATGGAGGAATTTGCAGAGATGGTGGCGCGTGGAGTTGGGGTTGTGAGGGATTTGAAGGAGGTGGCTCGGTGGTGCGAAGGGCTGGAACAGGGGAGGGTTGGGCTTGAGAGACTTGGAGAATTGCTGGGCGAGAAGCGGTTTGGGATGAAGCACCACGCTGGTTCGGATAGCTTGTTGACGGCTTTTGTGCATGCGAAGATGGCGCGGTGGTTCCGTTTGGAATCAGGAGCGTATGACGGGCATTTGTATGGGCTTCAGAACAAGCTCGAACACATGAAGGTGAATCAGACGATCATGTTTGGGCAGATTTCTTGTTCGTTGTGGGATTATGAATGA

Coding sequence (CDS)

ATGGGTAGAAGAAATCGAGCACGTCCTAGAAGAAGAACACCTTCGATCCAAGAAGTTTGGGCTTCCAATTTCGATCATGAAATCCTCCGCCTCGAACGCTGCCTTCAAGCTAACCATATTCTATCCATCGACACCGAATTTCCTGGGTGCATTAGGAGCACCCCATGGGGCTCGATCGATGAACGCGTCTACGAAGATCTCCGATTCAATGTGAACCAAACGAAGTTAATTCAACTGGGTTTAACAGCTTCTGATGAATCTGGGCAAGTCGTCGGCTCATGGGAGTTCAACTTCTCCGATTTCGACCATCAAATCGACACTCAAAATCCTTCATCGATTTCGTTTCTGGAACAGAACGGGCTCGATTTTGCGAAGCTCAAGAAACAGGGGATCCCCTTAAGCCTATTCACTCTAAACTTCTCGCGTATCATACGAAGAAGCCGTGTTCGTAAATGGGTCACATTCCATGGATTATACGATATTGGGTATCTTGTGAAGGCGATGGGGGTAGCGGAGGAGTTGCCGGAATCAATGGAGGAATTTGCAGAGATGGTGGCGCGTGGAGTTGGGGTTGTGAGGGATTTGAAGGAGGTGGCTCGGTGGTGCGAAGGGCTGGAACAGGGGAGGGTTGGGCTTGAGAGACTTGGAGAATTGCTGGGCGAGAAGCGGTTTGGGATGAAGCACCACGCTGGTTCGGATAGCTTGTTGACGGCTTTTGTGCATGCGAAGATGGCGCGGTGGTTCCGTTTGGAATCAGGAGCGTATGACGGGCATTTGTATGGGCTTCAGAACAAGCTCGAACACATGAAGGTGAATCAGACGATCATGTTTGGGCAGATTTCTTGTTCGTTGTGGGATTATGAATGA

Protein sequence

MGRRNRARPRRRTPSIQEVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSIDERVYEDLRFNVNQTKLIQLGLTASDESGQVVGSWEFNFSDFDHQIDTQNPSSISFLEQNGLDFAKLKKQGIPLSLFTLNFSRIIRRSRVRKWVTFHGLYDIGYLVKAMGVAEELPESMEEFAEMVARGVGVVRDLKEVARWCEGLEQGRVGLERLGELLGEKRFGMKHHAGSDSLLTAFVHAKMARWFRLESGAYDGHLYGLQNKLEHMKVNQTIMFGQISCSLWDYE

Homology

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BLAST of Carg05169 vs. NCBI nr
Match: KAG6604931.1 (putative CCR4-associated factor 1-like 8, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027010.1 putative CCR4-associated factor 1-like 8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 593.2 bits (1528), Expect = 1.2e-165
Identity = 288/288 (100.00%), Postives = 288/288 (100.00%), Query Frame = 0


Query: 1   MGRRNRARPRRRTPSIQEVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSID 60
           MGRRNRARPRRRTPSIQEVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSID
Sbjct: 1   MGRRNRARPRRRTPSIQEVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSID 60

Query: 61  ERVYEDLRFNVNQTKLIQLGLTASDESGQVVGSWEFNFSDFDHQIDTQNPSSISFLEQNG 120
           ERVYEDLRFNVNQTKLIQLGLTASDESGQVVGSWEFNFSDFDHQIDTQNPSSISFLEQNG
Sbjct: 61  ERVYEDLRFNVNQTKLIQLGLTASDESGQVVGSWEFNFSDFDHQIDTQNPSSISFLEQNG 120

Query: 121 LDFAKLKKQGIPLSLFTLNFSRIIRRSRVRKWVTFHGLYDIGYLVKAMGVAEELPESMEE 180
           LDFAKLKKQGIPLSLFTLNFSRIIRRSRVRKWVTFHGLYDIGYLVKAMGVAEELPESMEE
Sbjct: 121 LDFAKLKKQGIPLSLFTLNFSRIIRRSRVRKWVTFHGLYDIGYLVKAMGVAEELPESMEE 180

Query: 181 FAEMVARGVGVVRDLKEVARWCEGLEQGRVGLERLGELLGEKRFGMKHHAGSDSLLTAFV 240
           FAEMVARGVGVVRDLKEVARWCEGLEQGRVGLERLGELLGEKRFGMKHHAGSDSLLTAFV
Sbjct: 181 FAEMVARGVGVVRDLKEVARWCEGLEQGRVGLERLGELLGEKRFGMKHHAGSDSLLTAFV 240

Query: 241 HAKMARWFRLESGAYDGHLYGLQNKLEHMKVNQTIMFGQISCSLWDYE 289
           HAKMARWFRLESGAYDGHLYGLQNKLEHMKVNQTIMFGQISCSLWDYE
Sbjct: 241 HAKMARWFRLESGAYDGHLYGLQNKLEHMKVNQTIMFGQISCSLWDYE 288

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BLAST of Carg05169 vs. NCBI nr
Match: XP_022947867.1 (putative CCR4-associated factor 1 homolog 8 [Cucurbita moschata])

HSP 1 Score: 539.7 bits (1389), Expect = 1.6e-149
Identity = 262/274 (95.62%), Postives = 268/274 (97.81%), Query Frame = 0


Query: 1   MGRRNRARPRRRTPSIQEVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSID 60
           MGRRNR RPRRRTPSIQEVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSID
Sbjct: 1   MGRRNRERPRRRTPSIQEVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSID 60

Query: 61  ERVYEDLRFNVNQTKLIQLGLTASDESGQVVGSWEFNFSDFDHQIDTQNPSSISFLEQNG 120
           ERVYEDLRFNVNQTKLIQLGLTASDESGQV GSWEFNFSDFDHQIDTQNPSSI+FLEQNG
Sbjct: 61  ERVYEDLRFNVNQTKLIQLGLTASDESGQVAGSWEFNFSDFDHQIDTQNPSSIAFLEQNG 120

Query: 121 LDFAKLKKQGIPLSLFTLNFSRIIRRSRVRKWVTFHGLYDIGYLVKAMGVAEELPESMEE 180
           LDFAKLKKQGIPLSLFTLNFSRIIRRSRVRKWVTFHGLYDIGYLVKAMGVAEELPESMEE
Sbjct: 121 LDFAKLKKQGIPLSLFTLNFSRIIRRSRVRKWVTFHGLYDIGYLVKAMGVAEELPESMEE 180

Query: 181 FAEMVARGVGVVRDLKEVARWCEGLEQGRVGLERLGELLGEKRFGMKHHAGSDSLLTAFV 240
           FAEMVARGVGVVRDLKEVARWCEGLEQGRVGLERLGELLGEKRFGMKHHAGSDSLLTAFV
Sbjct: 181 FAEMVARGVGVVRDLKEVARWCEGLEQGRVGLERLGELLGEKRFGMKHHAGSDSLLTAFV 240

Query: 241 HAKMARWFRLESGAYDGHLYGLQNKLEHMKVNQT 275
           HAKMARWFRLES AYDGHLYGL+NKL++M   ++
Sbjct: 241 HAKMARWFRLESEAYDGHLYGLRNKLQYMNEGES 274

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BLAST of Carg05169 vs. NCBI nr
Match: XP_023533171.1 (putative CCR4-associated factor 1 homolog 8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 506.5 bits (1303), Expect = 1.5e-139
Identity = 248/253 (98.02%), Postives = 249/253 (98.42%), Query Frame = 0


Query: 1   MGRRNRARPRRRTPSIQEVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSID 60
           MGRRNRARPRRRTPSIQEVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSID
Sbjct: 1   MGRRNRARPRRRTPSIQEVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSID 60

Query: 61  ERVYEDLRFNVNQTKLIQLGLTASDESGQVVGSWEFNFSDFDHQIDTQNPSSISFLEQNG 120
           ERVYEDLRFNVNQTKLIQLGLTASDESGQV GSWEFNFSDFDHQIDTQNPSSISFLEQNG
Sbjct: 61  ERVYEDLRFNVNQTKLIQLGLTASDESGQVAGSWEFNFSDFDHQIDTQNPSSISFLEQNG 120

Query: 121 LDFAKLKKQGIPLSLFTLNFSRIIRRSRVRKWVTFHGLYDIGYLVKAMGVAEELPESMEE 180
           LDFAKLKKQGIPLSLFTL FSRIIRRSRVRKWVTFHGLYDIGYLVKAMGVAEELPESMEE
Sbjct: 121 LDFAKLKKQGIPLSLFTLKFSRIIRRSRVRKWVTFHGLYDIGYLVKAMGVAEELPESMEE 180

Query: 181 FAEMVARGVGVVRDLKEVARWCEGLEQGRVGLERLGELLGEKRFGMKHHAGSDSLLTAFV 240
           FAEMVARGVGVVRDLKEVARWCEGLEQG+VGLERLGELLGEKRFGMKHHAGSDSLLTAFV
Sbjct: 181 FAEMVARGVGVVRDLKEVARWCEGLEQGKVGLERLGELLGEKRFGMKHHAGSDSLLTAFV 240

Query: 241 HAKMARWFRLESG 254
           HAKMARW  LESG
Sbjct: 241 HAKMARWLGLESG 253

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BLAST of Carg05169 vs. NCBI nr
Match: XP_038895744.1 (putative CCR4-associated factor 1 homolog 8 [Benincasa hispida])

HSP 1 Score: 372.9 bits (956), Expect = 2.6e-99
Identity = 180/277 (64.98%), Postives = 221/277 (79.78%), Query Frame = 0


Query: 10  RRRTPSIQEVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSIDERVYEDLRF 69
           R R   I +VWASNFD EI +LE CL+ + I+SIDTEFPG IR++PWGSIDE +YED RF
Sbjct: 5   RIRKSLIHKVWASNFDAEISKLEECLEFHTIVSIDTEFPGFIRNSPWGSIDETLYEDFRF 64

Query: 70  NVNQTKLIQLGLTASDESGQVVGSWEFNFSDFDHQIDTQNPSSISFLEQNGLDFAKLKKQ 129
           NVNQTKLIQLGLTA D+SG++ GSWEFNFSDFD +ID Q+PSS SFLEQNGLDF KLKK 
Sbjct: 65  NVNQTKLIQLGLTACDDSGKIGGSWEFNFSDFDPEIDAQSPSSASFLEQNGLDFNKLKKY 124

Query: 130 GIPLSLFTLNFSRIIRRSRVRKWVTFHGLYDIGYLVKAMGVAEELPESMEEFAEMVARGV 189
           GIP++LFT  F  IIR+  V +WVTFHGLYDIGYL+KAMG+ E LPE+MEEFA +V R +
Sbjct: 125 GIPINLFTAKFVHIIRKHNVFRWVTFHGLYDIGYLIKAMGITEVLPETMEEFATVVVRRL 184

Query: 190 GVVRDLKEVARWCEGLEQGRVGLERLGELLGEKRFGMKHHAGSDSLLTAFVHAKMARWFR 249
           G+VRDLK +AR+CEGLE G++GLERLG+LL +KR+G+KH+AGSDSLLTA  H +M + F 
Sbjct: 185 GIVRDLKHMARFCEGLEDGKLGLERLGKLLDQKRYGLKHNAGSDSLLTASAHFEMIQRFD 244

Query: 250 LESGAYDGHLYGLQNKLEHMKVN------QTIMFGQI 281
           +++   DG+LYG  NKL+ M +N      + I FGQI
Sbjct: 245 MDAEICDGYLYGFSNKLQSMDINMNMFHRKIITFGQI 281

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BLAST of Carg05169 vs. NCBI nr
Match: XP_022146113.1 (putative CCR4-associated factor 1 homolog 8 [Momordica charantia])

HSP 1 Score: 358.2 bits (918), Expect = 6.5e-95
Identity = 177/285 (62.11%), Postives = 217/285 (76.14%), Query Frame = 0


Query: 1   MGRRNRARPRRRTPSIQEVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSID 60
           MGR  R   RR    I+EVW+ NFD E+  L  CL    IL+IDTEFPG IRSTPWG+I+
Sbjct: 1   MGRGKRGHSRRGNAQIREVWSRNFDLELANLNDCLNLYPILTIDTEFPGFIRSTPWGAIE 60

Query: 61  ERVYEDLRFNVNQTKLIQLGLTASDESGQVVGSWEFNFSDFDHQIDTQNPSSISFLEQNG 120
           E++Y+D RFNVN+TKLIQLGLTASDE G +  +WEFNFSDFD QIDT N SS+SFL+QNG
Sbjct: 61  EKLYDDFRFNVNRTKLIQLGLTASDEIGNIGSTWEFNFSDFDPQIDTHNASSVSFLKQNG 120

Query: 121 LDFAKLKKQGIPLSLFTLNFSRIIRRSRVRKWVTFHGLYDIGYLVKAMGVAEELPESMEE 180
           LDF KLK+ GIP++ FT     + R+ R+ +WVTFHGLYDIGYL+KAMGVA E+PESMEE
Sbjct: 121 LDFDKLKEFGIPINEFTSKAFFLFRKHRIVRWVTFHGLYDIGYLLKAMGVAGEIPESMEE 180

Query: 181 FAEMVARGVGVVRDLKEVARWCEGLEQGRVGLERLGELLGEKRFGMKHHAGSDSLLTAFV 240
           FAE+V R +G V DLK +A++CEGLE G++GLE+L +L+ +KRFG KHHAGSDSLLTA +
Sbjct: 181 FAELVERALGNVSDLKHMAQFCEGLEDGKLGLEKLAQLMNQKRFGNKHHAGSDSLLTASL 240

Query: 241 HAKMARWFRLESGAYDGHLYGLQNKLE-HMKVNQTIMFGQISCSL 285
           +AKM   F L S   DG LYGL+ KL   +K+ Q +MFGQI   L
Sbjct: 241 YAKMKNQFELGSEVCDGFLYGLRIKLHAKIKLYQNLMFGQIPYGL 285

[Close]

BLAST of Carg05169 vs. ExPASy Swiss-Prot
Match: Q9LXM4 (Putative CCR4-associated factor 1 homolog 8 OS=Arabidopsis thaliana OX=3702 GN=CAF1-8 PE=3 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 6.4e-45
Identity = 101/221 (45.70%), Postives = 138/221 (62.44%), Query Frame = 0


Query: 31  LERCLQANHILSIDTEFPGCIRSTPWGSIDERVYEDLRFNVNQTKLIQLGLTASDESGQV 90
           +E CL++   ++IDTEFP  +R T   + DE  Y D+ F+V++ KLIQLGLT  D +G++
Sbjct: 4   IEDCLRSYRFIAIDTEFPSTLRETTQHATDEERYMDMSFSVDRAKLIQLGLTLFDINGRI 63

Query: 91  VGSWEFNFSDFDHQIDTQNPSSISFLEQNGLDFAKLKKQGIPLSLFTLNFSRIIRRSRVR 150
            G+WE NFSDF    D +N  SI FL +NGLD  K++++GI +  F      +++++R  
Sbjct: 64  GGTWEINFSDFGVD-DARNEKSIEFLRRNGLDLRKIREEGIRIEGFFSEMFWMLKKTRRN 123

Query: 151 -KWVTFHGLYDIGYLVKAMGVAEELPESMEEFAEMVARGVGVVRDLKEVARWCEGLEQGR 210
             WVTFHG YDI YL+K     E LP + E F++ VAR +G V DLK +A  CEGL   R
Sbjct: 124 ITWVTFHGSYDIAYLLKGF-TGEALPVTSERFSKAVARVLGSVYDLKVMAGRCEGL-SSR 183

Query: 211 VGLERLGELLGEKRFGMKHHAGSDSLLTAFVHAKMARWFRL 251
           +GLE L    G  R G  HHAGS++ LTA V AK+   F L
Sbjct: 184 LGLETLAHEFGLNRVGTAHHAGSNNELTAMVFAKVLSPFPL 221

[Close]

BLAST of Carg05169 vs. ExPASy Swiss-Prot
Match: O64773 (Probable CCR4-associated factor 1 homolog 5 OS=Arabidopsis thaliana OX=3702 GN=CAF1-5 PE=2 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 8.4e-45
Identity = 98/229 (42.79%), Postives = 143/229 (62.45%), Query Frame = 0


Query: 18  EVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSIDERVYEDLRFNVNQTKLI 77
           EVW  N   E+  +  CL+  + ++IDTEFPGC++ TP  + DE  Y D++FNV+ T LI
Sbjct: 4   EVWRWNKQAEMNSIRDCLKHCNSIAIDTEFPGCLKETPMDASDEIRYRDMKFNVDNTHLI 63

Query: 78  QLGLTASDESGQVVGSWEFNFSDFDHQIDTQNPSSISFLEQNGLDFAKLKKQGIPLSLFT 137
           QLGLT   +   +  +WE N SDF+     +N  SI+FL+ NGLD  K++++GI +  F 
Sbjct: 64  QLGLTLFGKG--ITKTWEINLSDFNESKSLKNDKSIAFLKNNGLDLDKIREEGIGIEEFF 123

Query: 138 LNFSRIIRRSRVR-KWVTFHGLYDIGYLVKAMGVAEELPESMEEFAEMVARGVG-VVRDL 197
           + FS+I+     + +WVTF G YD  YL+K +   + LPE+ +EF E V + +G  V D+
Sbjct: 124 MEFSQILNEKHGKMRWVTFQGSYDKAYLLKGL-TRKPLPETSKEFDETVQQLLGRFVYDV 183

Query: 198 KEVARWCEGLEQGRVGLERLGELLGEKRFGMKHHAGSDSLLTAFVHAKM 245
           K++A  C GL   R GL+R+ ++L  +R G  HHAGSDS LTA V  K+
Sbjct: 184 KKMAGLCSGL-SSRFGLQRIADVLQMRRVGKAHHAGSDSELTARVFTKL 228

[Close]

BLAST of Carg05169 vs. ExPASy Swiss-Prot
Match: Q9C6M9 (Putative CCR4-associated factor 1 homolog 4 OS=Arabidopsis thaliana OX=3702 GN=CAF1-4 PE=3 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.1e-44
Identity = 99/229 (43.23%), Postives = 141/229 (61.57%), Query Frame = 0


Query: 18  EVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSIDERVYEDLRFNVNQTKLI 77
           EVW  N + E+  +  CL+    ++IDTEFPGC++ TP  + +E  Y D++FNV+ T LI
Sbjct: 3   EVWRWNKEVEMDSIRDCLKHFSSIAIDTEFPGCLKETPMDASEEIRYRDMKFNVDNTHLI 62

Query: 78  QLGLTASDESGQVVGSWEFNFSDFDHQIDTQNPSSISFLEQNGLDFAKLKKQGIPLSLFT 137
           QLG T  D  G +  +WE N SDF+     +N  SI+FL+ NGL+  K+ ++GI +  F 
Sbjct: 63  QLGFTLFDRRG-ITKTWEINLSDFNEHKCFKNDKSIAFLKSNGLNLDKIGEEGIGIEEFF 122

Query: 138 LNFSRIIRRSRVR-KWVTFHGLYDIGYLVKAMGVAEELPESMEEFAEMVARGVG-VVRDL 197
            +FS+I++    +  WV F G YD  YLVK +   + LPE+ EEF E V + +G  V D+
Sbjct: 123 RDFSQILKEKDGKITWVNFQGSYDNAYLVKGLTGGKPLPETKEEFHETVEQLLGKFVFDV 182

Query: 198 KEVARWCEGLEQGRVGLERLGELLGEKRFGMKHHAGSDSLLTAFVHAKM 245
           K++A  C GL   R GL+R+ ++L  KR G  HHAGSDS LTA V  K+
Sbjct: 183 KKIAESCSGL-SSRFGLQRIADVLQMKRVGKAHHAGSDSELTARVFTKL 229

[Close]

BLAST of Carg05169 vs. ExPASy Swiss-Prot
Match: Q9SFX6 (Putative CCR4-associated factor 1 homolog 3 OS=Arabidopsis thaliana OX=3702 GN=CAF1-3 PE=3 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 1.4e-44
Identity = 99/229 (43.23%), Postives = 140/229 (61.14%), Query Frame = 0


Query: 18  EVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSIDERVYEDLRFNVNQTKLI 77
           EVW  N + E+  +  CL+    ++IDTEFPGC++ TP  + +E  Y D++FNV+ T LI
Sbjct: 8   EVWRWNKEVEMNSIRDCLKHCSSIAIDTEFPGCLKETPMDASEEIRYRDMKFNVDNTHLI 67

Query: 78  QLGLTASDESGQVVGSWEFNFSDFDHQIDTQNPSSISFLEQNGLDFAKLKKQGIPLSLFT 137
           QLG T  D  G    +WE N SDFD     +N  SI+FL+ NGL+  K++++GI +  F 
Sbjct: 68  QLGFTLFDRRG-FAKTWEINLSDFDEHKCFKNDKSIAFLKSNGLNLDKIREEGIGIDEFF 127

Query: 138 LNFSRIIRRSRVR-KWVTFHGLYDIGYLVKAMGVAEELPESMEEFAEMVARGVG-VVRDL 197
            +FS+I+     +  WV F G YD  YLVK +   + LPE+ EEF E V + +G  V D+
Sbjct: 128 RDFSQILTEKDGKITWVNFQGSYDNAYLVKGLTGGKPLPETKEEFHETVQQLLGKFVFDV 187

Query: 198 KEVARWCEGLEQGRVGLERLGELLGEKRFGMKHHAGSDSLLTAFVHAKM 245
           K++A  C GL   + GL+R+ ++L  KR G  HHAGSDS LTA V  K+
Sbjct: 188 KKIAESCSGL-SSQFGLQRIADVLQMKRVGKAHHAGSDSELTARVFTKL 234

[Close]

BLAST of Carg05169 vs. ExPASy Swiss-Prot
Match: Q9SHJ0 (Probable CCR4-associated factor 1 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=CAF1-1 PE=2 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 5.1e-42
Identity = 96/251 (38.25%), Postives = 141/251 (56.18%), Query Frame = 0


Query: 14  PSIQEVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSIDERVYEDLRFNVNQ 73
           P  + VW SN D E+ R+  CL+   +++ DTE+PG I  T + S  +  Y  ++ NV  
Sbjct: 7   PLARRVWRSNVDEEMARMAECLKRFPLIAFDTEYPGIIFRTYFDSSSDECYRAMKGNVEN 66

Query: 74  TKLIQLGLTASDESGQVVGSWEFNFSDFDHQIDTQNPSSISFLEQNGLDFAKLKKQGIPL 133
           TKLIQ G T  +  G++ G WE NFS+F    DT+N  SI FL ++GLD  K++ +G+ +
Sbjct: 67  TKLIQCGFTLFNAKGEIGGVWEINFSNFGDPSDTRNELSIEFLRRHGLDLQKIRDEGVDM 126

Query: 134 --SLFTLNFSRIIRRSRVRKWVTFHGLYDIGYLVKAMGVAEELPESMEEFAEMVARGVGV 193
               F      + R  +  ++VTF G YD  Y +  +    +LPE+  EFA  V +  G 
Sbjct: 127 FGYGFFPKLMTVFRSQKHVEFVTFQGAYDFAYFLSILNHG-KLPETHGEFATEVVKVFGQ 186

Query: 194 VRDLKEVARWCEGLEQGRVGLERLGELLGEKRFGMKHHAGSDSLLTAFVHAKMARWFRLE 253
           V D K +A +CEGL +  +GL +L +LL   R G  HHAGSDSL+TA V  K+   +  +
Sbjct: 187 VYDTKVMAGFCEGLGE-HLGLSKLAQLLQITRVGRAHHAGSDSLMTALVFIKLKHVYE-D 246

Query: 254 SGAYDGHLYGL 263
           S    G +YG+
Sbjct: 247 SRFARGLIYGI 254

[Close]

BLAST of Carg05169 vs. ExPASy TrEMBL
Match: A0A6J1G7L2 (Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111451625 PE=3 SV=1)

HSP 1 Score: 539.7 bits (1389), Expect = 7.7e-150
Identity = 262/274 (95.62%), Postives = 268/274 (97.81%), Query Frame = 0


Query: 1   MGRRNRARPRRRTPSIQEVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSID 60
           MGRRNR RPRRRTPSIQEVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSID
Sbjct: 1   MGRRNRERPRRRTPSIQEVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSID 60

Query: 61  ERVYEDLRFNVNQTKLIQLGLTASDESGQVVGSWEFNFSDFDHQIDTQNPSSISFLEQNG 120
           ERVYEDLRFNVNQTKLIQLGLTASDESGQV GSWEFNFSDFDHQIDTQNPSSI+FLEQNG
Sbjct: 61  ERVYEDLRFNVNQTKLIQLGLTASDESGQVAGSWEFNFSDFDHQIDTQNPSSIAFLEQNG 120

Query: 121 LDFAKLKKQGIPLSLFTLNFSRIIRRSRVRKWVTFHGLYDIGYLVKAMGVAEELPESMEE 180
           LDFAKLKKQGIPLSLFTLNFSRIIRRSRVRKWVTFHGLYDIGYLVKAMGVAEELPESMEE
Sbjct: 121 LDFAKLKKQGIPLSLFTLNFSRIIRRSRVRKWVTFHGLYDIGYLVKAMGVAEELPESMEE 180

Query: 181 FAEMVARGVGVVRDLKEVARWCEGLEQGRVGLERLGELLGEKRFGMKHHAGSDSLLTAFV 240
           FAEMVARGVGVVRDLKEVARWCEGLEQGRVGLERLGELLGEKRFGMKHHAGSDSLLTAFV
Sbjct: 181 FAEMVARGVGVVRDLKEVARWCEGLEQGRVGLERLGELLGEKRFGMKHHAGSDSLLTAFV 240

Query: 241 HAKMARWFRLESGAYDGHLYGLQNKLEHMKVNQT 275
           HAKMARWFRLES AYDGHLYGL+NKL++M   ++
Sbjct: 241 HAKMARWFRLESEAYDGHLYGLRNKLQYMNEGES 274

[Close]

BLAST of Carg05169 vs. ExPASy TrEMBL
Match: A0A6J1CXP9 (Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111015407 PE=3 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 3.2e-95
Identity = 177/285 (62.11%), Postives = 217/285 (76.14%), Query Frame = 0


Query: 1   MGRRNRARPRRRTPSIQEVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSID 60
           MGR  R   RR    I+EVW+ NFD E+  L  CL    IL+IDTEFPG IRSTPWG+I+
Sbjct: 1   MGRGKRGHSRRGNAQIREVWSRNFDLELANLNDCLNLYPILTIDTEFPGFIRSTPWGAIE 60

Query: 61  ERVYEDLRFNVNQTKLIQLGLTASDESGQVVGSWEFNFSDFDHQIDTQNPSSISFLEQNG 120
           E++Y+D RFNVN+TKLIQLGLTASDE G +  +WEFNFSDFD QIDT N SS+SFL+QNG
Sbjct: 61  EKLYDDFRFNVNRTKLIQLGLTASDEIGNIGSTWEFNFSDFDPQIDTHNASSVSFLKQNG 120

Query: 121 LDFAKLKKQGIPLSLFTLNFSRIIRRSRVRKWVTFHGLYDIGYLVKAMGVAEELPESMEE 180
           LDF KLK+ GIP++ FT     + R+ R+ +WVTFHGLYDIGYL+KAMGVA E+PESMEE
Sbjct: 121 LDFDKLKEFGIPINEFTSKAFFLFRKHRIVRWVTFHGLYDIGYLLKAMGVAGEIPESMEE 180

Query: 181 FAEMVARGVGVVRDLKEVARWCEGLEQGRVGLERLGELLGEKRFGMKHHAGSDSLLTAFV 240
           FAE+V R +G V DLK +A++CEGLE G++GLE+L +L+ +KRFG KHHAGSDSLLTA +
Sbjct: 181 FAELVERALGNVSDLKHMAQFCEGLEDGKLGLEKLAQLMNQKRFGNKHHAGSDSLLTASL 240

Query: 241 HAKMARWFRLESGAYDGHLYGLQNKLE-HMKVNQTIMFGQISCSL 285
           +AKM   F L S   DG LYGL+ KL   +K+ Q +MFGQI   L
Sbjct: 241 YAKMKNQFELGSEVCDGFLYGLRIKLHAKIKLYQNLMFGQIPYGL 285

[Close]

BLAST of Carg05169 vs. ExPASy TrEMBL
Match: A0A6J1G7T4 (Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111451626 PE=3 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 2.5e-92
Identity = 165/276 (59.78%), Postives = 207/276 (75.00%), Query Frame = 0


Query: 9   PRRRTPSIQEVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSIDERVYEDLR 68
           PR   P I+EVWA N DHEI+RL+RCL +  I+SIDTEFPGCI+ TPWG+ DE +Y D R
Sbjct: 8   PRGDNPLIREVWAYNLDHEIIRLDRCLLSYSIMSIDTEFPGCIKRTPWGTFDEELYADFR 67

Query: 69  FNVNQTKLIQLGLTASDESGQVVGSWEFNFSDFDHQIDTQNPSSISFLEQNGLDFAKLKK 128
           FNVNQTK+IQLG+T SDESG + G+WEFNFSDFD +ID  NP+SI FL+QNGLDF KLKK
Sbjct: 68  FNVNQTKVIQLGVTVSDESGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGLDFGKLKK 127

Query: 129 QGIPLSLFTLNFSRIIRRSRVRKWVTFHGLYDIGYLVKAMGVAEELPESMEEFAEMVARG 188
            GI +  F + F   + +  + +WVTFHGLYDIGYL+ AMGV + LPE++ EF  +VAR 
Sbjct: 128 DGIKVRKFAIRFLYTLGKHAIYRWVTFHGLYDIGYLMLAMGVVKSLPETLGEFKWIVARR 187

Query: 189 VGVVRDLKEVARWCEGLEQGRVGLERLGELLGEKRFGMKHHAGSDSLLTAFVHAKMARWF 248
           VG VRDLK +AR+CEGLE G +GLE+LG+LL +KRFG+KHHAGSDSL+TA +H KM + +
Sbjct: 188 VGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLHEKMLQRY 247

Query: 249 RLESGAYDGHLYGLQNKLEHMKVNQT-IMFGQISCS 284
              +   DG LYGL  K E +   QT  ++ QI C+
Sbjct: 248 DFHAEICDGFLYGLSKKFEELVGLQTHRIYVQIQCA 283

[Close]

BLAST of Carg05169 vs. ExPASy TrEMBL
Match: A0A6J1I6H7 (Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111469633 PE=3 SV=1)

HSP 1 Score: 343.6 bits (880), Expect = 8.1e-91
Identity = 162/276 (58.70%), Postives = 204/276 (73.91%), Query Frame = 0


Query: 9   PRRRTPSIQEVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSIDERVYEDLR 68
           PR   P I EVWA N D EILRL+ CL    ++SIDTEFPGCI+ TPWG+IDE +Y D R
Sbjct: 8   PRGDNPLITEVWAYNLDDEILRLDHCLLLYPVMSIDTEFPGCIKRTPWGTIDEELYADFR 67

Query: 69  FNVNQTKLIQLGLTASDESGQVVGSWEFNFSDFDHQIDTQNPSSISFLEQNGLDFAKLKK 128
           FNVNQTK+IQLG+T SDESG + G+WEFNFSDFD +ID  NP+SI FL+QNGLDF KLKK
Sbjct: 68  FNVNQTKVIQLGVTVSDESGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGLDFGKLKK 127

Query: 129 QGIPLSLFTLNFSRIIRRSRVRKWVTFHGLYDIGYLVKAMGVAEELPESMEEFAEMVARG 188
            GI +  F + F   +R+  + +W+TFHGLYDIGYL+ A+GV + LPE++ EF  +VAR 
Sbjct: 128 DGIKVRKFAIRFLYTMRKHAIHQWITFHGLYDIGYLILALGVVKSLPETLGEFEWIVARR 187

Query: 189 VGVVRDLKEVARWCEGLEQGRVGLERLGELLGEKRFGMKHHAGSDSLLTAFVHAKMARWF 248
           VG VRDLK +AR+CEGLE G +GLE+LG+LL +KRFG+KHHAGSDSL+TA +H KM + +
Sbjct: 188 VGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLHEKMLQLY 247

Query: 249 RLESGAYDGHLYGLQNKLEHMKVNQT-IMFGQISCS 284
              +   DG LYGL  K E     Q+  ++ QI C+
Sbjct: 248 DFNAEICDGFLYGLSKKFEEFVGMQSHRIYVQIKCA 283

[Close]

BLAST of Carg05169 vs. ExPASy TrEMBL
Match: A0A6J1I4S7 (Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111469631 PE=3 SV=1)

HSP 1 Score: 343.6 bits (880), Expect = 8.1e-91
Identity = 162/276 (58.70%), Postives = 204/276 (73.91%), Query Frame = 0


Query: 9   PRRRTPSIQEVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSIDERVYEDLR 68
           PR   P I EVWA N D EILRL+ CL    ++SIDTEFPGCI+ TPWG+IDE +Y D R
Sbjct: 8   PRGDNPLITEVWAYNLDDEILRLDHCLLLYPVMSIDTEFPGCIKRTPWGTIDEELYADFR 67

Query: 69  FNVNQTKLIQLGLTASDESGQVVGSWEFNFSDFDHQIDTQNPSSISFLEQNGLDFAKLKK 128
           FNVNQTK+IQLG+T SDESG + G+WEFNFSDFD +ID  NP+SI FL+QNGLDF KLKK
Sbjct: 68  FNVNQTKVIQLGVTVSDESGDIGGTWEFNFSDFDPEIDAHNPASICFLKQNGLDFGKLKK 127

Query: 129 QGIPLSLFTLNFSRIIRRSRVRKWVTFHGLYDIGYLVKAMGVAEELPESMEEFAEMVARG 188
            GI +  F + F   +R+  + +W+TFHGLYDIGYL+ A+GV + LPE++ EF  +VAR 
Sbjct: 128 DGIKVRKFAIRFLYTMRKHAIHQWITFHGLYDIGYLILALGVVKSLPETLGEFEWIVARR 187

Query: 189 VGVVRDLKEVARWCEGLEQGRVGLERLGELLGEKRFGMKHHAGSDSLLTAFVHAKMARWF 248
           VG VRDLK +AR+CEGLE G +GLE+LG+LL +KRFG+KHHAGSDSL+TA +H KM + +
Sbjct: 188 VGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLHEKMLQLY 247

Query: 249 RLESGAYDGHLYGLQNKLEHMKVNQT-IMFGQISCS 284
              +   DG LYGL  K E     Q+  ++ QI C+
Sbjct: 248 DFNAEICDGFLYGLSKKFEEFVGMQSHRIYVQIKCA 283

[Close]

BLAST of Carg05169 vs. TAIR 10
Match: AT3G44240.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 182.6 bits (462), Expect = 4.6e-46
Identity = 101/221 (45.70%), Postives = 138/221 (62.44%), Query Frame = 0


Query: 31  LERCLQANHILSIDTEFPGCIRSTPWGSIDERVYEDLRFNVNQTKLIQLGLTASDESGQV 90
           +E CL++   ++IDTEFP  +R T   + DE  Y D+ F+V++ KLIQLGLT  D +G++
Sbjct: 4   IEDCLRSYRFIAIDTEFPSTLRETTQHATDEERYMDMSFSVDRAKLIQLGLTLFDINGRI 63

Query: 91  VGSWEFNFSDFDHQIDTQNPSSISFLEQNGLDFAKLKKQGIPLSLFTLNFSRIIRRSRVR 150
            G+WE NFSDF    D +N  SI FL +NGLD  K++++GI +  F      +++++R  
Sbjct: 64  GGTWEINFSDFGVD-DARNEKSIEFLRRNGLDLRKIREEGIRIEGFFSEMFWMLKKTRRN 123

Query: 151 -KWVTFHGLYDIGYLVKAMGVAEELPESMEEFAEMVARGVGVVRDLKEVARWCEGLEQGR 210
             WVTFHG YDI YL+K     E LP + E F++ VAR +G V DLK +A  CEGL   R
Sbjct: 124 ITWVTFHGSYDIAYLLKGF-TGEALPVTSERFSKAVARVLGSVYDLKVMAGRCEGL-SSR 183

Query: 211 VGLERLGELLGEKRFGMKHHAGSDSLLTAFVHAKMARWFRL 251
           +GLE L    G  R G  HHAGS++ LTA V AK+   F L
Sbjct: 184 LGLETLAHEFGLNRVGTAHHAGSNNELTAMVFAKVLSPFPL 221

[Close]

BLAST of Carg05169 vs. TAIR 10
Match: AT1G61470.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 182.2 bits (461), Expect = 6.0e-46
Identity = 98/229 (42.79%), Postives = 143/229 (62.45%), Query Frame = 0


Query: 18  EVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSIDERVYEDLRFNVNQTKLI 77
           EVW  N   E+  +  CL+  + ++IDTEFPGC++ TP  + DE  Y D++FNV+ T LI
Sbjct: 4   EVWRWNKQAEMNSIRDCLKHCNSIAIDTEFPGCLKETPMDASDEIRYRDMKFNVDNTHLI 63

Query: 78  QLGLTASDESGQVVGSWEFNFSDFDHQIDTQNPSSISFLEQNGLDFAKLKKQGIPLSLFT 137
           QLGLT   +   +  +WE N SDF+     +N  SI+FL+ NGLD  K++++GI +  F 
Sbjct: 64  QLGLTLFGKG--ITKTWEINLSDFNESKSLKNDKSIAFLKNNGLDLDKIREEGIGIEEFF 123

Query: 138 LNFSRIIRRSRVR-KWVTFHGLYDIGYLVKAMGVAEELPESMEEFAEMVARGVG-VVRDL 197
           + FS+I+     + +WVTF G YD  YL+K +   + LPE+ +EF E V + +G  V D+
Sbjct: 124 MEFSQILNEKHGKMRWVTFQGSYDKAYLLKGL-TRKPLPETSKEFDETVQQLLGRFVYDV 183

Query: 198 KEVARWCEGLEQGRVGLERLGELLGEKRFGMKHHAGSDSLLTAFVHAKM 245
           K++A  C GL   R GL+R+ ++L  +R G  HHAGSDS LTA V  K+
Sbjct: 184 KKMAGLCSGL-SSRFGLQRIADVLQMRRVGKAHHAGSDSELTARVFTKL 228

[Close]

BLAST of Carg05169 vs. TAIR 10
Match: AT1G27890.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 181.8 bits (460), Expect = 7.8e-46
Identity = 99/229 (43.23%), Postives = 141/229 (61.57%), Query Frame = 0


Query: 18  EVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSIDERVYEDLRFNVNQTKLI 77
           EVW  N + E+  +  CL+    ++IDTEFPGC++ TP  + +E  Y D++FNV+ T LI
Sbjct: 3   EVWRWNKEVEMDSIRDCLKHFSSIAIDTEFPGCLKETPMDASEEIRYRDMKFNVDNTHLI 62

Query: 78  QLGLTASDESGQVVGSWEFNFSDFDHQIDTQNPSSISFLEQNGLDFAKLKKQGIPLSLFT 137
           QLG T  D  G +  +WE N SDF+     +N  SI+FL+ NGL+  K+ ++GI +  F 
Sbjct: 63  QLGFTLFDRRG-ITKTWEINLSDFNEHKCFKNDKSIAFLKSNGLNLDKIGEEGIGIEEFF 122

Query: 138 LNFSRIIRRSRVR-KWVTFHGLYDIGYLVKAMGVAEELPESMEEFAEMVARGVG-VVRDL 197
            +FS+I++    +  WV F G YD  YLVK +   + LPE+ EEF E V + +G  V D+
Sbjct: 123 RDFSQILKEKDGKITWVNFQGSYDNAYLVKGLTGGKPLPETKEEFHETVEQLLGKFVFDV 182

Query: 198 KEVARWCEGLEQGRVGLERLGELLGEKRFGMKHHAGSDSLLTAFVHAKM 245
           K++A  C GL   R GL+R+ ++L  KR G  HHAGSDS LTA V  K+
Sbjct: 183 KKIAESCSGL-SSRFGLQRIADVLQMKRVGKAHHAGSDSELTARVFTKL 229

[Close]

BLAST of Carg05169 vs. TAIR 10
Match: AT1G27820.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 181.4 bits (459), Expect = 1.0e-45
Identity = 99/229 (43.23%), Postives = 140/229 (61.14%), Query Frame = 0


Query: 18  EVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSIDERVYEDLRFNVNQTKLI 77
           EVW  N + E+  +  CL+    ++IDTEFPGC++ TP  + +E  Y D++FNV+ T LI
Sbjct: 8   EVWRWNKEVEMNSIRDCLKHCSSIAIDTEFPGCLKETPMDASEEIRYRDMKFNVDNTHLI 67

Query: 78  QLGLTASDESGQVVGSWEFNFSDFDHQIDTQNPSSISFLEQNGLDFAKLKKQGIPLSLFT 137
           QLG T  D  G    +WE N SDFD     +N  SI+FL+ NGL+  K++++GI +  F 
Sbjct: 68  QLGFTLFDRRG-FAKTWEINLSDFDEHKCFKNDKSIAFLKSNGLNLDKIREEGIGIDEFF 127

Query: 138 LNFSRIIRRSRVR-KWVTFHGLYDIGYLVKAMGVAEELPESMEEFAEMVARGVG-VVRDL 197
            +FS+I+     +  WV F G YD  YLVK +   + LPE+ EEF E V + +G  V D+
Sbjct: 128 RDFSQILTEKDGKITWVNFQGSYDNAYLVKGLTGGKPLPETKEEFHETVQQLLGKFVFDV 187

Query: 198 KEVARWCEGLEQGRVGLERLGELLGEKRFGMKHHAGSDSLLTAFVHAKM 245
           K++A  C GL   + GL+R+ ++L  KR G  HHAGSDS LTA V  K+
Sbjct: 188 KKIAESCSGL-SSQFGLQRIADVLQMKRVGKAHHAGSDSELTARVFTKL 234

[Close]

BLAST of Carg05169 vs. TAIR 10
Match: AT1G06450.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 172.9 bits (437), Expect = 3.6e-43
Identity = 96/251 (38.25%), Postives = 141/251 (56.18%), Query Frame = 0


Query: 14  PSIQEVWASNFDHEILRLERCLQANHILSIDTEFPGCIRSTPWGSIDERVYEDLRFNVNQ 73
           P  + VW SN D E+ R+  CL+   +++ DTE+PG I  T + S  +  Y  ++ NV  
Sbjct: 7   PLARRVWRSNVDEEMARMAECLKRFPLIAFDTEYPGIIFRTYFDSSSDECYRAMKGNVEN 66

Query: 74  TKLIQLGLTASDESGQVVGSWEFNFSDFDHQIDTQNPSSISFLEQNGLDFAKLKKQGIPL 133
           TKLIQ G T  +  G++ G WE NFS+F    DT+N  SI FL ++GLD  K++ +G+ +
Sbjct: 67  TKLIQCGFTLFNAKGEIGGVWEINFSNFGDPSDTRNELSIEFLRRHGLDLQKIRDEGVDM 126

Query: 134 --SLFTLNFSRIIRRSRVRKWVTFHGLYDIGYLVKAMGVAEELPESMEEFAEMVARGVGV 193
               F      + R  +  ++VTF G YD  Y +  +    +LPE+  EFA  V +  G 
Sbjct: 127 FGYGFFPKLMTVFRSQKHVEFVTFQGAYDFAYFLSILNHG-KLPETHGEFATEVVKVFGQ 186

Query: 194 VRDLKEVARWCEGLEQGRVGLERLGELLGEKRFGMKHHAGSDSLLTAFVHAKMARWFRLE 253
           V D K +A +CEGL +  +GL +L +LL   R G  HHAGSDSL+TA V  K+   +  +
Sbjct: 187 VYDTKVMAGFCEGLGE-HLGLSKLAQLLQITRVGRAHHAGSDSLMTALVFIKLKHVYE-D 246

Query: 254 SGAYDGHLYGL 263
           S    G +YG+
Sbjct: 247 SRFARGLIYGI 254

The following BLAST results are available for this feature:

BLAST of Carg05169 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6604931.1	1.2e-165	100.00	putative CCR4-associated factor 1-like 8, partial [Cucurbita argyrosperma subsp....	[more]
XP_022947867.1	1.6e-149	95.62	putative CCR4-associated factor 1 homolog 8 [Cucurbita moschata]	[more]
XP_023533171.1	1.5e-139	98.02	putative CCR4-associated factor 1 homolog 8 [Cucurbita pepo subsp. pepo]	[more]
XP_038895744.1	2.6e-99	64.98	putative CCR4-associated factor 1 homolog 8 [Benincasa hispida]	[more]
XP_022146113.1	6.5e-95	62.11	putative CCR4-associated factor 1 homolog 8 [Momordica charantia]	[more]

BLAST of Carg05169 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LXM4	6.4e-45	45.70	Putative CCR4-associated factor 1 homolog 8 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
O64773	8.4e-45	42.79	Probable CCR4-associated factor 1 homolog 5 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
Q9C6M9	1.1e-44	43.23	Putative CCR4-associated factor 1 homolog 4 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
Q9SFX6	1.4e-44	43.23	Putative CCR4-associated factor 1 homolog 3 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]
Q9SHJ0	5.1e-42	38.25	Probable CCR4-associated factor 1 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=C...	[more]

BLAST of Carg05169 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G7L2	7.7e-150	95.62	Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111451625 PE=3...	[more]
A0A6J1CXP9	3.2e-95	62.11	Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111015407 PE=...	[more]
A0A6J1G7T4	2.5e-92	59.78	Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111451626 PE=3...	[more]
A0A6J1I6H7	8.1e-91	58.70	Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111469633 PE=3 S...	[more]
A0A6J1I4S7	8.1e-91	58.70	Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111469631 PE=3 S...	[more]

BLAST of Carg05169 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G44240.1	4.6e-46	45.70	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G61470.1	6.0e-46	42.79	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G27890.1	7.8e-46	43.23	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G27820.1	1.0e-45	43.23	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G06450.1	3.6e-43	38.25	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 4..266 e-value: 3.7E-67 score: 228.2
IPR006941	Ribonuclease CAF1	PFAM	PF04857	CAF1	coord: 19..132 e-value: 6.1E-11 score: 42.2
None	No IPR available	PANTHER	PTHR10797:SF57	CCR4-ASSOCIATED FACTOR 1 HOMOLOG 3-RELATED	coord: 14..273
IPR039637	CCR4-NOT transcription complex subunit 7/8/Pop2	PANTHER	PTHR10797	CCR4-NOT TRANSCRIPTION COMPLEX SUBUNIT	coord: 14..273
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 10..262

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg05169-RA	Carg05169-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006402	mRNA catabolic process
biological_process	GO:0090503	RNA phosphodiester bond hydrolysis, exonucleolytic
cellular_component	GO:0030014	CCR4-NOT complex
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004535	poly(A)-specific ribonuclease activity