Carg05168 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg05168
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionPoly(A)-specific ribonuclease
LocationCarg_Chr07: 3285590 .. 3286639 (-)
RNA-Seq ExpressionCarg05168
SyntenyCarg05168
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATAGAATTGTTCGAGGCCCGAGAGGAGACAACCCTTTGATTAGAGAGGTATGGGCTTACAATCTTGATCATGAAATCATTCGGCTCGACCGTTGTTTGGTATCCTACTCAGTTATGTCGATCGATACGGAATTTCCTGGGTGTATTAAGAGAACTCCTTGGGGCACGATTGATGAAGAACTCTACGCAGACTTTCGATTCAATGTCAACCAAACCAAGGTGATTCAACTAGGTGTGACAGTTTCTGATGAATTAGGGAACATCGGCGGTACATGGGAGTTTAATTTCTCTGACTTCGATCCTGAAATCGACGCTCATAACCCTGCGTCGATTTGTTTTCTGAAACAGAACGGCTTGGATTTTGGAAAACTTAAAAAAGATGGAATTAAGGTTAGAAAATTCGCAATAAGATTCTTATATACGTTGCGAAAGCATGCGATATACCGGTGGATTACATTTCACGGATTATACGATATTGGATATCTGATGTTGGCGATGGGCGTGGTTAAAAGCTTGCCGGAAACATTGGGAGAATTCGAATGGATCGTGGCGAGGAGAGTGGGGACCGTGAGGGATTTAAAGCATATGGCGCGATTCTGCGAAGGGCTCGAAGGGGGCAATTTAGGACTCGAGAAATTAGGGCAGTTGCTGGACCAAAAACGCTTTGGATTGAAGCATCACGCTGGTTCTGATAGTTTAATGACTGCGCTGCTTCATGAGAAGATGTTGCAACGGTATGATTTCCATGCGGAAATTTGCGATGGGTTTTTGTATGGGCTATCGAAGAAATTCGAGGAGCTCGTGGGCTTGCAAACACATCGCATATATGTTCAAATTCAGTGTGCGAGAGTGAAAGCTATGGTGATTCATAAGAAGATGTTACAACTGTTTGAGGTGGAAAGTTGCAATGAATATGAGCTGTCGAAGAAATTAAAGGAGTTCGAGGTCTTGATATCACACTTCAGATTTGTTGAACACGAGTATGAGACGGCGCAATTTTGTTATTACAAGGCTTGCAACATTATGTACCAAGGATTTCATCTTTGA

mRNA sequence

ATGGATAGAATTGTTCGAGGCCCGAGAGGAGACAACCCTTTGATTAGAGAGGTATGGGCTTACAATCTTGATCATGAAATCATTCGGCTCGACCGTTGTTTGGTATCCTACTCAGTTATGTCGATCGATACGGAATTTCCTGGGTGTATTAAGAGAACTCCTTGGGGCACGATTGATGAAGAACTCTACGCAGACTTTCGATTCAATGTCAACCAAACCAAGGTGATTCAACTAGGTGTGACAGTTTCTGATGAATTAGGGAACATCGGCGGTACATGGGAGTTTAATTTCTCTGACTTCGATCCTGAAATCGACGCTCATAACCCTGCGTCGATTTGTTTTCTGAAACAGAACGGCTTGGATTTTGGAAAACTTAAAAAAGATGGAATTAAGGTTAGAAAATTCGCAATAAGATTCTTATATACGTTGCGAAAGCATGCGATATACCGGTGGATTACATTTCACGGATTATACGATATTGGATATCTGATGTTGGCGATGGGCGTGGTTAAAAGCTTGCCGGAAACATTGGGAGAATTCGAATGGATCGTGGCGAGGAGAGTGGGGACCGTGAGGGATTTAAAGCATATGGCGCGATTCTGCGAAGGGCTCGAAGGGGGCAATTTAGGACTCGAGAAATTAGGGCAGTTGCTGGACCAAAAACGCTTTGGATTGAAGCATCACGCTGGTTCTGATAGTTTAATGACTGCGCTGCTTCATGAGAAGATGTTGCAACGGTATGATTTCCATGCGGAAATTTGCGATGGGTTTTTGTATGGGCTATCGAAGAAATTCGAGGAGCTCGTGGGCTTGCAAACACATCGCATATATGTTCAAATTCAGTGTGCGAGAGTGAAAGCTATGGTGATTCATAAGAAGATGTTACAACTGTTTGAGGTGGAAAGTTGCAATGAATATGAGCTGTCGAAGAAATTAAAGGAGTTCGAGGTCTTGATATCACACTTCAGATTTGTTGAACACGAGTATGAGACGGCGCAATTTTGTTATTACAAGGCTTGCAACATTATGTACCAAGGATTTCATCTTTGA

Coding sequence (CDS)

ATGGATAGAATTGTTCGAGGCCCGAGAGGAGACAACCCTTTGATTAGAGAGGTATGGGCTTACAATCTTGATCATGAAATCATTCGGCTCGACCGTTGTTTGGTATCCTACTCAGTTATGTCGATCGATACGGAATTTCCTGGGTGTATTAAGAGAACTCCTTGGGGCACGATTGATGAAGAACTCTACGCAGACTTTCGATTCAATGTCAACCAAACCAAGGTGATTCAACTAGGTGTGACAGTTTCTGATGAATTAGGGAACATCGGCGGTACATGGGAGTTTAATTTCTCTGACTTCGATCCTGAAATCGACGCTCATAACCCTGCGTCGATTTGTTTTCTGAAACAGAACGGCTTGGATTTTGGAAAACTTAAAAAAGATGGAATTAAGGTTAGAAAATTCGCAATAAGATTCTTATATACGTTGCGAAAGCATGCGATATACCGGTGGATTACATTTCACGGATTATACGATATTGGATATCTGATGTTGGCGATGGGCGTGGTTAAAAGCTTGCCGGAAACATTGGGAGAATTCGAATGGATCGTGGCGAGGAGAGTGGGGACCGTGAGGGATTTAAAGCATATGGCGCGATTCTGCGAAGGGCTCGAAGGGGGCAATTTAGGACTCGAGAAATTAGGGCAGTTGCTGGACCAAAAACGCTTTGGATTGAAGCATCACGCTGGTTCTGATAGTTTAATGACTGCGCTGCTTCATGAGAAGATGTTGCAACGGTATGATTTCCATGCGGAAATTTGCGATGGGTTTTTGTATGGGCTATCGAAGAAATTCGAGGAGCTCGTGGGCTTGCAAACACATCGCATATATGTTCAAATTCAGTGTGCGAGAGTGAAAGCTATGGTGATTCATAAGAAGATGTTACAACTGTTTGAGGTGGAAAGTTGCAATGAATATGAGCTGTCGAAGAAATTAAAGGAGTTCGAGGTCTTGATATCACACTTCAGATTTGTTGAACACGAGTATGAGACGGCGCAATTTTGTTATTACAAGGCTTGCAACATTATGTACCAAGGATTTCATCTTTGA

Protein sequence

MDRIVRGPRGDNPLIREVWAYNLDHEIIRLDRCLVSYSVMSIDTEFPGCIKRTPWGTIDEELYADFRFNVNQTKVIQLGVTVSDELGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGLDFGKLKKDGIKVRKFAIRFLYTLRKHAIYRWITFHGLYDIGYLMLAMGVVKSLPETLGEFEWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLHEKMLQRYDFHAEICDGFLYGLSKKFEELVGLQTHRIYVQIQCARVKAMVIHKKMLQLFEVESCNEYELSKKLKEFEVLISHFRFVEHEYETAQFCYYKACNIMYQGFHL
Homology
BLAST of Carg05168 vs. NCBI nr
Match: KAG7027009.1 (putative CCR4-associated factor 1-like 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 726.5 bits (1874), Expect = 1.1e-205
Identity = 349/349 (100.00%), Postives = 349/349 (100.00%), Query Frame = 0

Query: 1   MDRIVRGPRGDNPLIREVWAYNLDHEIIRLDRCLVSYSVMSIDTEFPGCIKRTPWGTIDE 60
           MDRIVRGPRGDNPLIREVWAYNLDHEIIRLDRCLVSYSVMSIDTEFPGCIKRTPWGTIDE
Sbjct: 1   MDRIVRGPRGDNPLIREVWAYNLDHEIIRLDRCLVSYSVMSIDTEFPGCIKRTPWGTIDE 60

Query: 61  ELYADFRFNVNQTKVIQLGVTVSDELGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 120
           ELYADFRFNVNQTKVIQLGVTVSDELGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL
Sbjct: 61  ELYADFRFNVNQTKVIQLGVTVSDELGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 120

Query: 121 DFGKLKKDGIKVRKFAIRFLYTLRKHAIYRWITFHGLYDIGYLMLAMGVVKSLPETLGEF 180
           DFGKLKKDGIKVRKFAIRFLYTLRKHAIYRWITFHGLYDIGYLMLAMGVVKSLPETLGEF
Sbjct: 121 DFGKLKKDGIKVRKFAIRFLYTLRKHAIYRWITFHGLYDIGYLMLAMGVVKSLPETLGEF 180

Query: 181 EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH 240
           EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH
Sbjct: 181 EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH 240

Query: 241 EKMLQRYDFHAEICDGFLYGLSKKFEELVGLQTHRIYVQIQCARVKAMVIHKKMLQLFEV 300
           EKMLQRYDFHAEICDGFLYGLSKKFEELVGLQTHRIYVQIQCARVKAMVIHKKMLQLFEV
Sbjct: 241 EKMLQRYDFHAEICDGFLYGLSKKFEELVGLQTHRIYVQIQCARVKAMVIHKKMLQLFEV 300

Query: 301 ESCNEYELSKKLKEFEVLISHFRFVEHEYETAQFCYYKACNIMYQGFHL 350
           ESCNEYELSKKLKEFEVLISHFRFVEHEYETAQFCYYKACNIMYQGFHL
Sbjct: 301 ESCNEYELSKKLKEFEVLISHFRFVEHEYETAQFCYYKACNIMYQGFHL 349

BLAST of Carg05168 vs. NCBI nr
Match: KAG6604930.1 (putative CCR4-associated factor 1-like 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 725.7 bits (1872), Expect = 1.9e-205
Identity = 348/349 (99.71%), Postives = 349/349 (100.00%), Query Frame = 0

Query: 1   MDRIVRGPRGDNPLIREVWAYNLDHEIIRLDRCLVSYSVMSIDTEFPGCIKRTPWGTIDE 60
           MDRIVRGPRGDNPLIREVWAYNLDHEIIRLDRCLVSYSVMSIDTEFPGCIKRTPWGTIDE
Sbjct: 1   MDRIVRGPRGDNPLIREVWAYNLDHEIIRLDRCLVSYSVMSIDTEFPGCIKRTPWGTIDE 60

Query: 61  ELYADFRFNVNQTKVIQLGVTVSDELGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 120
           ELYADFRFNVNQTKVIQLGVTVSDELGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL
Sbjct: 61  ELYADFRFNVNQTKVIQLGVTVSDELGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 120

Query: 121 DFGKLKKDGIKVRKFAIRFLYTLRKHAIYRWITFHGLYDIGYLMLAMGVVKSLPETLGEF 180
           DFGKLKKDGIKVRKFAIRF+YTLRKHAIYRWITFHGLYDIGYLMLAMGVVKSLPETLGEF
Sbjct: 121 DFGKLKKDGIKVRKFAIRFIYTLRKHAIYRWITFHGLYDIGYLMLAMGVVKSLPETLGEF 180

Query: 181 EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH 240
           EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH
Sbjct: 181 EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH 240

Query: 241 EKMLQRYDFHAEICDGFLYGLSKKFEELVGLQTHRIYVQIQCARVKAMVIHKKMLQLFEV 300
           EKMLQRYDFHAEICDGFLYGLSKKFEELVGLQTHRIYVQIQCARVKAMVIHKKMLQLFEV
Sbjct: 241 EKMLQRYDFHAEICDGFLYGLSKKFEELVGLQTHRIYVQIQCARVKAMVIHKKMLQLFEV 300

Query: 301 ESCNEYELSKKLKEFEVLISHFRFVEHEYETAQFCYYKACNIMYQGFHL 350
           ESCNEYELSKKLKEFEVLISHFRFVEHEYETAQFCYYKACNIMYQGFHL
Sbjct: 301 ESCNEYELSKKLKEFEVLISHFRFVEHEYETAQFCYYKACNIMYQGFHL 349

BLAST of Carg05168 vs. NCBI nr
Match: XP_022947868.1 (putative CCR4-associated factor 1 homolog 8 [Cucurbita moschata])

HSP 1 Score: 712.6 bits (1838), Expect = 1.7e-201
Identity = 340/349 (97.42%), Postives = 345/349 (98.85%), Query Frame = 0

Query: 1   MDRIVRGPRGDNPLIREVWAYNLDHEIIRLDRCLVSYSVMSIDTEFPGCIKRTPWGTIDE 60
           MDRIVRGPRGDNPLIREVWAYNLDHEIIRLDRCL+SYS+MSIDTEFPGCIKRTPWGT DE
Sbjct: 1   MDRIVRGPRGDNPLIREVWAYNLDHEIIRLDRCLLSYSIMSIDTEFPGCIKRTPWGTFDE 60

Query: 61  ELYADFRFNVNQTKVIQLGVTVSDELGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 120
           ELYADFRFNVNQTKVIQLGVTVSDE GNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL
Sbjct: 61  ELYADFRFNVNQTKVIQLGVTVSDESGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 120

Query: 121 DFGKLKKDGIKVRKFAIRFLYTLRKHAIYRWITFHGLYDIGYLMLAMGVVKSLPETLGEF 180
           DFGKLKKDGIKVRKFAIRFLYTL KHAIYRW+TFHGLYDIGYLMLAMGVVKSLPETLGEF
Sbjct: 121 DFGKLKKDGIKVRKFAIRFLYTLGKHAIYRWVTFHGLYDIGYLMLAMGVVKSLPETLGEF 180

Query: 181 EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH 240
           +WIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH
Sbjct: 181 KWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH 240

Query: 241 EKMLQRYDFHAEICDGFLYGLSKKFEELVGLQTHRIYVQIQCARVKAMVIHKKMLQLFEV 300
           EKMLQRYDFHAEICDGFLYGLSKKFEELVGLQTHRIYVQIQCARVKAMVIHKKMLQLFEV
Sbjct: 241 EKMLQRYDFHAEICDGFLYGLSKKFEELVGLQTHRIYVQIQCARVKAMVIHKKMLQLFEV 300

Query: 301 ESCNEYELSKKLKEFEVLISHFRFVEHEYETAQFCYYKACNIMYQGFHL 350
           ESCNEYELSKKLKEFEVL SHF+FVEHEYETAQFCYYKACNIMYQGFHL
Sbjct: 301 ESCNEYELSKKLKEFEVLKSHFKFVEHEYETAQFCYYKACNIMYQGFHL 349

BLAST of Carg05168 vs. NCBI nr
Match: XP_023533172.1 (putative CCR4-associated factor 1 homolog 8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 709.1 bits (1829), Expect = 1.8e-200
Identity = 338/349 (96.85%), Postives = 345/349 (98.85%), Query Frame = 0

Query: 1   MDRIVRGPRGDNPLIREVWAYNLDHEIIRLDRCLVSYSVMSIDTEFPGCIKRTPWGTIDE 60
           MDRIVRGPRGDNPLIREVWAYNLDHEIIRLDRCL+SYSVMSIDTEFPGCIKRTPWGTIDE
Sbjct: 1   MDRIVRGPRGDNPLIREVWAYNLDHEIIRLDRCLLSYSVMSIDTEFPGCIKRTPWGTIDE 60

Query: 61  ELYADFRFNVNQTKVIQLGVTVSDELGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 120
           ELYADFRFNVNQTKVIQLGVTVSDE+GNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL
Sbjct: 61  ELYADFRFNVNQTKVIQLGVTVSDEVGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 120

Query: 121 DFGKLKKDGIKVRKFAIRFLYTLRKHAIYRWITFHGLYDIGYLMLAMGVVKSLPETLGEF 180
           DFGKLKKDGI+VRKFAIRFLYT+RKHAIYRW+TFHGLYDIGYLMLAMGVVKSLPETLGEF
Sbjct: 121 DFGKLKKDGIQVRKFAIRFLYTIRKHAIYRWVTFHGLYDIGYLMLAMGVVKSLPETLGEF 180

Query: 181 EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH 240
           EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH
Sbjct: 181 EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH 240

Query: 241 EKMLQRYDFHAEICDGFLYGLSKKFEELVGLQTHRIYVQIQCARVKAMVIHKKMLQLFEV 300
           EKMLQRYDF+AEICDGFLYGLSKKFEE VGLQTHRIYVQIQCARVKAMVIHKKMLQLF+V
Sbjct: 241 EKMLQRYDFNAEICDGFLYGLSKKFEEFVGLQTHRIYVQIQCARVKAMVIHKKMLQLFDV 300

Query: 301 ESCNEYELSKKLKEFEVLISHFRFVEHEYETAQFCYYKACNIMYQGFHL 350
           ESCNEYELSKKLKEFEVL SHFRFVEHEYE AQFCYY ACNIMYQGFHL
Sbjct: 301 ESCNEYELSKKLKEFEVLKSHFRFVEHEYEMAQFCYYNACNIMYQGFHL 349

BLAST of Carg05168 vs. NCBI nr
Match: XP_022970734.1 (putative CCR4-associated factor 1 homolog 8 [Cucurbita maxima] >XP_022970738.1 putative CCR4-associated factor 1 homolog 8 [Cucurbita maxima])

HSP 1 Score: 637.5 bits (1643), Expect = 6.8e-179
Identity = 306/345 (88.70%), Postives = 323/345 (93.62%), Query Frame = 0

Query: 1   MDRIVRGPRGDNPLIREVWAYNLDHEIIRLDRCLVSYSVMSIDTEFPGCIKRTPWGTIDE 60
           MDRIV+GPRGDNPLI EVWAYNLD EI+RLD CL+ Y VMSIDTEFPGCIKRTPWGTIDE
Sbjct: 1   MDRIVQGPRGDNPLITEVWAYNLDDEILRLDHCLLLYPVMSIDTEFPGCIKRTPWGTIDE 60

Query: 61  ELYADFRFNVNQTKVIQLGVTVSDELGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 120
           ELYADFRFNVNQTKVIQLGVTVSDE GNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL
Sbjct: 61  ELYADFRFNVNQTKVIQLGVTVSDESGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 120

Query: 121 DFGKLKKDGIKVRKFAIRFLYTLRKHAIYRWITFHGLYDIGYLMLAMGVVKSLPETLGEF 180
           DFGKLKKDGIKVRKFAIRFLYT+RKHAI++WITFHGLYDIGYL+LA+GVVKSLPETLGEF
Sbjct: 121 DFGKLKKDGIKVRKFAIRFLYTMRKHAIHQWITFHGLYDIGYLILALGVVKSLPETLGEF 180

Query: 181 EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH 240
           EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH
Sbjct: 181 EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH 240

Query: 241 EKMLQRYDFHAEICDGFLYGLSKKFEELVGLQTHRIYVQIQCARVKAMVIHKKMLQLFEV 300
           EKMLQ YDF+AEICDGFLYGLSKKFEE VG+Q+HRIYVQI+CA VKAMVI KKML+LF  
Sbjct: 241 EKMLQLYDFNAEICDGFLYGLSKKFEEFVGMQSHRIYVQIKCAEVKAMVIRKKMLKLFYA 300

Query: 301 ESCNEYELSKKLKEFEVLISHFRFVEHEYETAQFCYYKACNIMYQ 346
           + C+EYELSKK KEF+VL SH RFV+ E E  QF YYKA NIMYQ
Sbjct: 301 KICDEYELSKKFKEFKVLQSHLRFVQQECEIGQFYYYKASNIMYQ 345

BLAST of Carg05168 vs. ExPASy Swiss-Prot
Match: Q9SHJ0 (Probable CCR4-associated factor 1 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=CAF1-1 PE=2 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 8.9e-49
Identity = 106/253 (41.90%), Postives = 145/253 (57.31%), Query Frame = 0

Query: 13  PLIREVWAYNLDHEIIRLDRCLVSYSVMSIDTEFPGCIKRTPWGTIDEELYADFRFNVNQ 72
           PL R VW  N+D E+ R+  CL  + +++ DTE+PG I RT + +  +E Y   + NV  
Sbjct: 7   PLARRVWRSNVDEEMARMAECLKRFPLIAFDTEYPGIIFRTYFDSSSDECYRAMKGNVEN 66

Query: 73  TKVIQLGVTVSDELGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGLDFGKLKKDGIKV 132
           TK+IQ G T+ +  G IGG WE NFS+F    D  N  SI FL+++GLD  K++ +G+ +
Sbjct: 67  TKLIQCGFTLFNAKGEIGGVWEINFSNFGDPSDTRNELSIEFLRRHGLDLQKIRDEGVDM 126

Query: 133 RKFAI--RFLYTLRKHAIYRWITFHGLYDIGYLMLAMGVVKSLPETLGEFEWIVARRVGT 192
             +    + +   R      ++TF G YD  Y +  +   K LPET GEF   V +  G 
Sbjct: 127 FGYGFFPKLMTVFRSQKHVEFVTFQGAYDFAYFLSILNHGK-LPETHGEFATEVVKVFGQ 186

Query: 193 VRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLHEKMLQRYDFH 252
           V D K MA FCEGL G +LGL KL QLL   R G  HHAGSDSLMTAL+  K+   Y+  
Sbjct: 187 VYDTKVMAGFCEGL-GEHLGLSKLAQLLQITRVGRAHHAGSDSLMTALVFIKLKHVYE-D 246

Query: 253 AEICDGFLYGLSK 264
           +    G +YG+ K
Sbjct: 247 SRFARGLIYGIGK 256

BLAST of Carg05168 vs. ExPASy Swiss-Prot
Match: O64773 (Probable CCR4-associated factor 1 homolog 5 OS=Arabidopsis thaliana OX=3702 GN=CAF1-5 PE=2 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 1.0e-44
Identity = 98/230 (42.61%), Postives = 135/230 (58.70%), Query Frame = 0

Query: 17  EVWAYNLDHEIIRLDRCLVSYSVMSIDTEFPGCIKRTPWGTIDEELYADFRFNVNQTKVI 76
           EVW +N   E+  +  CL   + ++IDTEFPGC+K TP    DE  Y D +FNV+ T +I
Sbjct: 4   EVWRWNKQAEMNSIRDCLKHCNSIAIDTEFPGCLKETPMDASDEIRYRDMKFNVDNTHLI 63

Query: 77  QLGVTVSDELGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGLDFGKLKKDGIKVRKFA 136
           QLG+T+  +   I  TWE N SDF+      N  SI FLK NGLD  K++++GI + +F 
Sbjct: 64  QLGLTLFGK--GITKTWEINLSDFNESKSLKNDKSIAFLKNNGLDLDKIREEGIGIEEFF 123

Query: 137 IRFLYTL-RKHAIYRWITFHGLYDIGYLMLAMGVVKSLPETLGEFEWIVARRVGT-VRDL 196
           + F   L  KH   RW+TF G YD  YL+  +   K LPET  EF+  V + +G  V D+
Sbjct: 124 MEFSQILNEKHGKMRWVTFQGSYDKAYLLKGL-TRKPLPETSKEFDETVQQLLGRFVYDV 183

Query: 197 KHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLHEKML 245
           K MA  C GL     GL+++  +L  +R G  HHAGSDS +TA +  K++
Sbjct: 184 KKMAGLCSGL-SSRFGLQRIADVLQMRRVGKAHHAGSDSELTARVFTKLI 229

BLAST of Carg05168 vs. ExPASy Swiss-Prot
Match: Q9LXM4 (Putative CCR4-associated factor 1 homolog 8 OS=Arabidopsis thaliana OX=3702 GN=CAF1-8 PE=3 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 3.0e-44
Identity = 98/219 (44.75%), Postives = 134/219 (61.19%), Query Frame = 0

Query: 30  LDRCLVSYSVMSIDTEFPGCIKRTPWGTIDEELYADFRFNVNQTKVIQLGVTVSDELGNI 89
           ++ CL SY  ++IDTEFP  ++ T     DEE Y D  F+V++ K+IQLG+T+ D  G I
Sbjct: 4   IEDCLRSYRFIAIDTEFPSTLRETTQHATDEERYMDMSFSVDRAKLIQLGLTLFDINGRI 63

Query: 90  GGTWEFNFSDFDPEIDAHNPASICFLKQNGLDFGKLKKDGIKVRKFAIRFLYTLRK-HAI 149
           GGTWE NFSDF  + DA N  SI FL++NGLD  K++++GI++  F     + L+K    
Sbjct: 64  GGTWEINFSDFGVD-DARNEKSIEFLRRNGLDLRKIREEGIRIEGFFSEMFWMLKKTRRN 123

Query: 150 YRWITFHGLYDIGYLMLAMGVVKSLPETLGEFEWIVARRVGTVRDLKHMARFCEGLEGGN 209
             W+TFHG YDI YL+      ++LP T   F   VAR +G+V DLK MA  CEGL    
Sbjct: 124 ITWVTFHGSYDIAYLLKGF-TGEALPVTSERFSKAVARVLGSVYDLKVMAGRCEGL-SSR 183

Query: 210 LGLEKLGQLLDQKRFGLKHHAGSDSLMTALLHEKMLQRY 248
           LGLE L       R G  HHAGS++ +TA++  K+L  +
Sbjct: 184 LGLETLAHEFGLNRVGTAHHAGSNNELTAMVFAKVLSPF 219

BLAST of Carg05168 vs. ExPASy Swiss-Prot
Match: Q9C6M9 (Putative CCR4-associated factor 1 homolog 4 OS=Arabidopsis thaliana OX=3702 GN=CAF1-4 PE=3 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 3.6e-42
Identity = 96/229 (41.92%), Postives = 131/229 (57.21%), Query Frame = 0

Query: 17  EVWAYNLDHEIIRLDRCLVSYSVMSIDTEFPGCIKRTPWGTIDEELYADFRFNVNQTKVI 76
           EVW +N + E+  +  CL  +S ++IDTEFPGC+K TP    +E  Y D +FNV+ T +I
Sbjct: 3   EVWRWNKEVEMDSIRDCLKHFSSIAIDTEFPGCLKETPMDASEEIRYRDMKFNVDNTHLI 62

Query: 77  QLGVTVSDELGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGLDFGKLKKDGIKVRKFA 136
           QLG T+ D  G I  TWE N SDF+      N  SI FLK NGL+  K+ ++GI + +F 
Sbjct: 63  QLGFTLFDRRG-ITKTWEINLSDFNEHKCFKNDKSIAFLKSNGLNLDKIGEEGIGIEEFF 122

Query: 137 IRFLYTLR-KHAIYRWITFHGLYDIGYLMLAMGVVKSLPETLGEFEWIVARRVGT-VRDL 196
             F   L+ K     W+ F G YD  YL+  +   K LPET  EF   V + +G  V D+
Sbjct: 123 RDFSQILKEKDGKITWVNFQGSYDNAYLVKGLTGGKPLPETKEEFHETVEQLLGKFVFDV 182

Query: 197 KHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLHEKM 244
           K +A  C GL     GL+++  +L  KR G  HHAGSDS +TA +  K+
Sbjct: 183 KKIAESCSGL-SSRFGLQRIADVLQMKRVGKAHHAGSDSELTARVFTKL 229

BLAST of Carg05168 vs. ExPASy Swiss-Prot
Match: Q9SFX6 (Putative CCR4-associated factor 1 homolog 3 OS=Arabidopsis thaliana OX=3702 GN=CAF1-3 PE=3 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 6.2e-42
Identity = 96/229 (41.92%), Postives = 129/229 (56.33%), Query Frame = 0

Query: 17  EVWAYNLDHEIIRLDRCLVSYSVMSIDTEFPGCIKRTPWGTIDEELYADFRFNVNQTKVI 76
           EVW +N + E+  +  CL   S ++IDTEFPGC+K TP    +E  Y D +FNV+ T +I
Sbjct: 8   EVWRWNKEVEMNSIRDCLKHCSSIAIDTEFPGCLKETPMDASEEIRYRDMKFNVDNTHLI 67

Query: 77  QLGVTVSDELGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGLDFGKLKKDGIKVRKFA 136
           QLG T+ D  G    TWE N SDFD      N  SI FLK NGL+  K++++GI + +F 
Sbjct: 68  QLGFTLFDRRG-FAKTWEINLSDFDEHKCFKNDKSIAFLKSNGLNLDKIREEGIGIDEFF 127

Query: 137 IRFLYTL-RKHAIYRWITFHGLYDIGYLMLAMGVVKSLPETLGEFEWIVARRVGT-VRDL 196
             F   L  K     W+ F G YD  YL+  +   K LPET  EF   V + +G  V D+
Sbjct: 128 RDFSQILTEKDGKITWVNFQGSYDNAYLVKGLTGGKPLPETKEEFHETVQQLLGKFVFDV 187

Query: 197 KHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLHEKM 244
           K +A  C GL     GL+++  +L  KR G  HHAGSDS +TA +  K+
Sbjct: 188 KKIAESCSGL-SSQFGLQRIADVLQMKRVGKAHHAGSDSELTARVFTKL 234

BLAST of Carg05168 vs. ExPASy TrEMBL
Match: A0A6J1G7T4 (Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111451626 PE=3 SV=1)

HSP 1 Score: 712.6 bits (1838), Expect = 8.0e-202
Identity = 340/349 (97.42%), Postives = 345/349 (98.85%), Query Frame = 0

Query: 1   MDRIVRGPRGDNPLIREVWAYNLDHEIIRLDRCLVSYSVMSIDTEFPGCIKRTPWGTIDE 60
           MDRIVRGPRGDNPLIREVWAYNLDHEIIRLDRCL+SYS+MSIDTEFPGCIKRTPWGT DE
Sbjct: 1   MDRIVRGPRGDNPLIREVWAYNLDHEIIRLDRCLLSYSIMSIDTEFPGCIKRTPWGTFDE 60

Query: 61  ELYADFRFNVNQTKVIQLGVTVSDELGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 120
           ELYADFRFNVNQTKVIQLGVTVSDE GNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL
Sbjct: 61  ELYADFRFNVNQTKVIQLGVTVSDESGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 120

Query: 121 DFGKLKKDGIKVRKFAIRFLYTLRKHAIYRWITFHGLYDIGYLMLAMGVVKSLPETLGEF 180
           DFGKLKKDGIKVRKFAIRFLYTL KHAIYRW+TFHGLYDIGYLMLAMGVVKSLPETLGEF
Sbjct: 121 DFGKLKKDGIKVRKFAIRFLYTLGKHAIYRWVTFHGLYDIGYLMLAMGVVKSLPETLGEF 180

Query: 181 EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH 240
           +WIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH
Sbjct: 181 KWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH 240

Query: 241 EKMLQRYDFHAEICDGFLYGLSKKFEELVGLQTHRIYVQIQCARVKAMVIHKKMLQLFEV 300
           EKMLQRYDFHAEICDGFLYGLSKKFEELVGLQTHRIYVQIQCARVKAMVIHKKMLQLFEV
Sbjct: 241 EKMLQRYDFHAEICDGFLYGLSKKFEELVGLQTHRIYVQIQCARVKAMVIHKKMLQLFEV 300

Query: 301 ESCNEYELSKKLKEFEVLISHFRFVEHEYETAQFCYYKACNIMYQGFHL 350
           ESCNEYELSKKLKEFEVL SHF+FVEHEYETAQFCYYKACNIMYQGFHL
Sbjct: 301 ESCNEYELSKKLKEFEVLKSHFKFVEHEYETAQFCYYKACNIMYQGFHL 349

BLAST of Carg05168 vs. ExPASy TrEMBL
Match: A0A6J1I6H7 (Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111469633 PE=3 SV=1)

HSP 1 Score: 637.5 bits (1643), Expect = 3.3e-179
Identity = 306/345 (88.70%), Postives = 323/345 (93.62%), Query Frame = 0

Query: 1   MDRIVRGPRGDNPLIREVWAYNLDHEIIRLDRCLVSYSVMSIDTEFPGCIKRTPWGTIDE 60
           MDRIV+GPRGDNPLI EVWAYNLD EI+RLD CL+ Y VMSIDTEFPGCIKRTPWGTIDE
Sbjct: 1   MDRIVQGPRGDNPLITEVWAYNLDDEILRLDHCLLLYPVMSIDTEFPGCIKRTPWGTIDE 60

Query: 61  ELYADFRFNVNQTKVIQLGVTVSDELGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 120
           ELYADFRFNVNQTKVIQLGVTVSDE GNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL
Sbjct: 61  ELYADFRFNVNQTKVIQLGVTVSDESGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 120

Query: 121 DFGKLKKDGIKVRKFAIRFLYTLRKHAIYRWITFHGLYDIGYLMLAMGVVKSLPETLGEF 180
           DFGKLKKDGIKVRKFAIRFLYT+RKHAI++WITFHGLYDIGYL+LA+GVVKSLPETLGEF
Sbjct: 121 DFGKLKKDGIKVRKFAIRFLYTMRKHAIHQWITFHGLYDIGYLILALGVVKSLPETLGEF 180

Query: 181 EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH 240
           EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH
Sbjct: 181 EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH 240

Query: 241 EKMLQRYDFHAEICDGFLYGLSKKFEELVGLQTHRIYVQIQCARVKAMVIHKKMLQLFEV 300
           EKMLQ YDF+AEICDGFLYGLSKKFEE VG+Q+HRIYVQI+CA VKAMVI KKML+LF  
Sbjct: 241 EKMLQLYDFNAEICDGFLYGLSKKFEEFVGMQSHRIYVQIKCAEVKAMVIRKKMLKLFYA 300

Query: 301 ESCNEYELSKKLKEFEVLISHFRFVEHEYETAQFCYYKACNIMYQ 346
           + C+EYELSKK KEF+VL SH RFV+ E E  QF YYKA NIMYQ
Sbjct: 301 KICDEYELSKKFKEFKVLQSHLRFVQQECEIGQFYYYKASNIMYQ 345

BLAST of Carg05168 vs. ExPASy TrEMBL
Match: A0A6J1I4S7 (Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111469631 PE=3 SV=1)

HSP 1 Score: 635.6 bits (1638), Expect = 1.2e-178
Identity = 305/345 (88.41%), Postives = 323/345 (93.62%), Query Frame = 0

Query: 1   MDRIVRGPRGDNPLIREVWAYNLDHEIIRLDRCLVSYSVMSIDTEFPGCIKRTPWGTIDE 60
           MDRIV+GPRGDNPLI EVWAYNLD EI+RLD CL+ Y VMSIDTEFPGCIKRTPWGTIDE
Sbjct: 1   MDRIVQGPRGDNPLITEVWAYNLDDEILRLDHCLLLYPVMSIDTEFPGCIKRTPWGTIDE 60

Query: 61  ELYADFRFNVNQTKVIQLGVTVSDELGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 120
           ELYADFRFNVNQTKVIQLGVTVSDE G+IGGTWEFNFSDFDPEIDAHNPASICFLKQNGL
Sbjct: 61  ELYADFRFNVNQTKVIQLGVTVSDESGDIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 120

Query: 121 DFGKLKKDGIKVRKFAIRFLYTLRKHAIYRWITFHGLYDIGYLMLAMGVVKSLPETLGEF 180
           DFGKLKKDGIKVRKFAIRFLYT+RKHAI++WITFHGLYDIGYL+LA+GVVKSLPETLGEF
Sbjct: 121 DFGKLKKDGIKVRKFAIRFLYTMRKHAIHQWITFHGLYDIGYLILALGVVKSLPETLGEF 180

Query: 181 EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH 240
           EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH
Sbjct: 181 EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH 240

Query: 241 EKMLQRYDFHAEICDGFLYGLSKKFEELVGLQTHRIYVQIQCARVKAMVIHKKMLQLFEV 300
           EKMLQ YDF+AEICDGFLYGLSKKFEE VG+Q+HRIYVQI+CA VKAMVI KKML+LF  
Sbjct: 241 EKMLQLYDFNAEICDGFLYGLSKKFEEFVGMQSHRIYVQIKCAEVKAMVIRKKMLKLFYA 300

Query: 301 ESCNEYELSKKLKEFEVLISHFRFVEHEYETAQFCYYKACNIMYQ 346
           + C+EYELSKK KEF+VL SH RFV+ E E  QF YYKA NIMYQ
Sbjct: 301 KICDEYELSKKFKEFKVLQSHLRFVQQECEIGQFYYYKASNIMYQ 345

BLAST of Carg05168 vs. ExPASy TrEMBL
Match: A0A6J1I1E6 (putative CCR4-associated factor 1 homolog 8 OS=Cucurbita maxima OX=3661 GN=LOC111469635 PE=4 SV=1)

HSP 1 Score: 634.0 bits (1634), Expect = 3.6e-178
Identity = 305/345 (88.41%), Postives = 322/345 (93.33%), Query Frame = 0

Query: 1   MDRIVRGPRGDNPLIREVWAYNLDHEIIRLDRCLVSYSVMSIDTEFPGCIKRTPWGTIDE 60
           MDRIV+GPRGDNPLI EVWAYNLD EI+RLD CL+ Y VMSIDTEFPGCIKRTPWGTIDE
Sbjct: 1   MDRIVQGPRGDNPLITEVWAYNLDDEILRLDHCLLLYPVMSIDTEFPGCIKRTPWGTIDE 60

Query: 61  ELYADFRFNVNQTKVIQLGVTVSDELGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 120
           ELYADFRFNVNQTKVIQL VTVSDE GNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL
Sbjct: 61  ELYADFRFNVNQTKVIQLCVTVSDESGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 120

Query: 121 DFGKLKKDGIKVRKFAIRFLYTLRKHAIYRWITFHGLYDIGYLMLAMGVVKSLPETLGEF 180
           DFGKLKKDGIKVRKFAIRFLYT+RKHAI++WITFHGLYDIGYL+LA+GVVKSLPETLGEF
Sbjct: 121 DFGKLKKDGIKVRKFAIRFLYTMRKHAIHQWITFHGLYDIGYLILALGVVKSLPETLGEF 180

Query: 181 EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH 240
           EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH
Sbjct: 181 EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH 240

Query: 241 EKMLQRYDFHAEICDGFLYGLSKKFEELVGLQTHRIYVQIQCARVKAMVIHKKMLQLFEV 300
           EKMLQ YDF+AEICDGFLYGLSKKFEE VG+Q+HRIYVQI+CA VKAMVI KKML+LF  
Sbjct: 241 EKMLQLYDFNAEICDGFLYGLSKKFEEFVGMQSHRIYVQIKCAEVKAMVIRKKMLKLFYA 300

Query: 301 ESCNEYELSKKLKEFEVLISHFRFVEHEYETAQFCYYKACNIMYQ 346
           + C+EYELSKK KEF+VL SH RFV+ E E  QF YYKA NIMYQ
Sbjct: 301 KICDEYELSKKFKEFKVLQSHLRFVQQECEIGQFYYYKASNIMYQ 345

BLAST of Carg05168 vs. ExPASy TrEMBL
Match: A0A6J1I3Q4 (Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111469634 PE=3 SV=1)

HSP 1 Score: 601.3 bits (1549), Expect = 2.6e-168
Identity = 286/317 (90.22%), Postives = 302/317 (95.27%), Query Frame = 0

Query: 1   MDRIVRGPRGDNPLIREVWAYNLDHEIIRLDRCLVSYSVMSIDTEFPGCIKRTPWGTIDE 60
           MDRIV+GPRGDNPLI EVWAYNLD EI+RLD CL+ Y VMSIDTEFPGCIKRTPWGTIDE
Sbjct: 1   MDRIVQGPRGDNPLITEVWAYNLDDEILRLDHCLLLYPVMSIDTEFPGCIKRTPWGTIDE 60

Query: 61  ELYADFRFNVNQTKVIQLGVTVSDELGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 120
           ELYADFRFNVNQTKVIQLGVTVSDE GNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL
Sbjct: 61  ELYADFRFNVNQTKVIQLGVTVSDESGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGL 120

Query: 121 DFGKLKKDGIKVRKFAIRFLYTLRKHAIYRWITFHGLYDIGYLMLAMGVVKSLPETLGEF 180
           DFGKLKKDGIKVRKFAIRFLYT+RKHAI++WITFHGLYDIGYL+LA+GVVKSLPETLGEF
Sbjct: 121 DFGKLKKDGIKVRKFAIRFLYTMRKHAIHQWITFHGLYDIGYLILALGVVKSLPETLGEF 180

Query: 181 EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLH 240
           EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMT LLH
Sbjct: 181 EWIVARRVGTVRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTVLLH 240

Query: 241 EKMLQRYDFHAEICDGFLYGLSKKFEELVGLQTHRIYVQIQCARVKAMVIHKKMLQLFEV 300
           EKMLQ YDF+AEICDGFLYGLSKKFEE VG+Q+HRIYVQI+CA VKAMVI KKML+LF  
Sbjct: 241 EKMLQLYDFNAEICDGFLYGLSKKFEEFVGMQSHRIYVQIKCAEVKAMVIRKKMLKLFYA 300

Query: 301 ESCNEYELSKKLKEFEV 318
           + C+EYELSKK KEF+V
Sbjct: 301 KICDEYELSKKFKEFKV 317

BLAST of Carg05168 vs. TAIR 10
Match: AT1G06450.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 195.7 bits (496), Expect = 6.3e-50
Identity = 106/253 (41.90%), Postives = 145/253 (57.31%), Query Frame = 0

Query: 13  PLIREVWAYNLDHEIIRLDRCLVSYSVMSIDTEFPGCIKRTPWGTIDEELYADFRFNVNQ 72
           PL R VW  N+D E+ R+  CL  + +++ DTE+PG I RT + +  +E Y   + NV  
Sbjct: 7   PLARRVWRSNVDEEMARMAECLKRFPLIAFDTEYPGIIFRTYFDSSSDECYRAMKGNVEN 66

Query: 73  TKVIQLGVTVSDELGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGLDFGKLKKDGIKV 132
           TK+IQ G T+ +  G IGG WE NFS+F    D  N  SI FL+++GLD  K++ +G+ +
Sbjct: 67  TKLIQCGFTLFNAKGEIGGVWEINFSNFGDPSDTRNELSIEFLRRHGLDLQKIRDEGVDM 126

Query: 133 RKFAI--RFLYTLRKHAIYRWITFHGLYDIGYLMLAMGVVKSLPETLGEFEWIVARRVGT 192
             +    + +   R      ++TF G YD  Y +  +   K LPET GEF   V +  G 
Sbjct: 127 FGYGFFPKLMTVFRSQKHVEFVTFQGAYDFAYFLSILNHGK-LPETHGEFATEVVKVFGQ 186

Query: 193 VRDLKHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLHEKMLQRYDFH 252
           V D K MA FCEGL G +LGL KL QLL   R G  HHAGSDSLMTAL+  K+   Y+  
Sbjct: 187 VYDTKVMAGFCEGL-GEHLGLSKLAQLLQITRVGRAHHAGSDSLMTALVFIKLKHVYE-D 246

Query: 253 AEICDGFLYGLSK 264
           +    G +YG+ K
Sbjct: 247 SRFARGLIYGIGK 256

BLAST of Carg05168 vs. TAIR 10
Match: AT1G61470.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 182.2 bits (461), Expect = 7.3e-46
Identity = 98/230 (42.61%), Postives = 135/230 (58.70%), Query Frame = 0

Query: 17  EVWAYNLDHEIIRLDRCLVSYSVMSIDTEFPGCIKRTPWGTIDEELYADFRFNVNQTKVI 76
           EVW +N   E+  +  CL   + ++IDTEFPGC+K TP    DE  Y D +FNV+ T +I
Sbjct: 4   EVWRWNKQAEMNSIRDCLKHCNSIAIDTEFPGCLKETPMDASDEIRYRDMKFNVDNTHLI 63

Query: 77  QLGVTVSDELGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGLDFGKLKKDGIKVRKFA 136
           QLG+T+  +   I  TWE N SDF+      N  SI FLK NGLD  K++++GI + +F 
Sbjct: 64  QLGLTLFGK--GITKTWEINLSDFNESKSLKNDKSIAFLKNNGLDLDKIREEGIGIEEFF 123

Query: 137 IRFLYTL-RKHAIYRWITFHGLYDIGYLMLAMGVVKSLPETLGEFEWIVARRVGT-VRDL 196
           + F   L  KH   RW+TF G YD  YL+  +   K LPET  EF+  V + +G  V D+
Sbjct: 124 MEFSQILNEKHGKMRWVTFQGSYDKAYLLKGL-TRKPLPETSKEFDETVQQLLGRFVYDV 183

Query: 197 KHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLHEKML 245
           K MA  C GL     GL+++  +L  +R G  HHAGSDS +TA +  K++
Sbjct: 184 KKMAGLCSGL-SSRFGLQRIADVLQMRRVGKAHHAGSDSELTARVFTKLI 229

BLAST of Carg05168 vs. TAIR 10
Match: AT3G44240.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 180.6 bits (457), Expect = 2.1e-45
Identity = 98/219 (44.75%), Postives = 134/219 (61.19%), Query Frame = 0

Query: 30  LDRCLVSYSVMSIDTEFPGCIKRTPWGTIDEELYADFRFNVNQTKVIQLGVTVSDELGNI 89
           ++ CL SY  ++IDTEFP  ++ T     DEE Y D  F+V++ K+IQLG+T+ D  G I
Sbjct: 4   IEDCLRSYRFIAIDTEFPSTLRETTQHATDEERYMDMSFSVDRAKLIQLGLTLFDINGRI 63

Query: 90  GGTWEFNFSDFDPEIDAHNPASICFLKQNGLDFGKLKKDGIKVRKFAIRFLYTLRK-HAI 149
           GGTWE NFSDF  + DA N  SI FL++NGLD  K++++GI++  F     + L+K    
Sbjct: 64  GGTWEINFSDFGVD-DARNEKSIEFLRRNGLDLRKIREEGIRIEGFFSEMFWMLKKTRRN 123

Query: 150 YRWITFHGLYDIGYLMLAMGVVKSLPETLGEFEWIVARRVGTVRDLKHMARFCEGLEGGN 209
             W+TFHG YDI YL+      ++LP T   F   VAR +G+V DLK MA  CEGL    
Sbjct: 124 ITWVTFHGSYDIAYLLKGF-TGEALPVTSERFSKAVARVLGSVYDLKVMAGRCEGL-SSR 183

Query: 210 LGLEKLGQLLDQKRFGLKHHAGSDSLMTALLHEKMLQRY 248
           LGLE L       R G  HHAGS++ +TA++  K+L  +
Sbjct: 184 LGLETLAHEFGLNRVGTAHHAGSNNELTAMVFAKVLSPF 219

BLAST of Carg05168 vs. TAIR 10
Match: AT1G27890.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 173.7 bits (439), Expect = 2.6e-43
Identity = 96/229 (41.92%), Postives = 131/229 (57.21%), Query Frame = 0

Query: 17  EVWAYNLDHEIIRLDRCLVSYSVMSIDTEFPGCIKRTPWGTIDEELYADFRFNVNQTKVI 76
           EVW +N + E+  +  CL  +S ++IDTEFPGC+K TP    +E  Y D +FNV+ T +I
Sbjct: 3   EVWRWNKEVEMDSIRDCLKHFSSIAIDTEFPGCLKETPMDASEEIRYRDMKFNVDNTHLI 62

Query: 77  QLGVTVSDELGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGLDFGKLKKDGIKVRKFA 136
           QLG T+ D  G I  TWE N SDF+      N  SI FLK NGL+  K+ ++GI + +F 
Sbjct: 63  QLGFTLFDRRG-ITKTWEINLSDFNEHKCFKNDKSIAFLKSNGLNLDKIGEEGIGIEEFF 122

Query: 137 IRFLYTLR-KHAIYRWITFHGLYDIGYLMLAMGVVKSLPETLGEFEWIVARRVGT-VRDL 196
             F   L+ K     W+ F G YD  YL+  +   K LPET  EF   V + +G  V D+
Sbjct: 123 RDFSQILKEKDGKITWVNFQGSYDNAYLVKGLTGGKPLPETKEEFHETVEQLLGKFVFDV 182

Query: 197 KHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLHEKM 244
           K +A  C GL     GL+++  +L  KR G  HHAGSDS +TA +  K+
Sbjct: 183 KKIAESCSGL-SSRFGLQRIADVLQMKRVGKAHHAGSDSELTARVFTKL 229

BLAST of Carg05168 vs. TAIR 10
Match: AT1G27820.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 172.9 bits (437), Expect = 4.4e-43
Identity = 96/229 (41.92%), Postives = 129/229 (56.33%), Query Frame = 0

Query: 17  EVWAYNLDHEIIRLDRCLVSYSVMSIDTEFPGCIKRTPWGTIDEELYADFRFNVNQTKVI 76
           EVW +N + E+  +  CL   S ++IDTEFPGC+K TP    +E  Y D +FNV+ T +I
Sbjct: 8   EVWRWNKEVEMNSIRDCLKHCSSIAIDTEFPGCLKETPMDASEEIRYRDMKFNVDNTHLI 67

Query: 77  QLGVTVSDELGNIGGTWEFNFSDFDPEIDAHNPASICFLKQNGLDFGKLKKDGIKVRKFA 136
           QLG T+ D  G    TWE N SDFD      N  SI FLK NGL+  K++++GI + +F 
Sbjct: 68  QLGFTLFDRRG-FAKTWEINLSDFDEHKCFKNDKSIAFLKSNGLNLDKIREEGIGIDEFF 127

Query: 137 IRFLYTL-RKHAIYRWITFHGLYDIGYLMLAMGVVKSLPETLGEFEWIVARRVGT-VRDL 196
             F   L  K     W+ F G YD  YL+  +   K LPET  EF   V + +G  V D+
Sbjct: 128 RDFSQILTEKDGKITWVNFQGSYDNAYLVKGLTGGKPLPETKEEFHETVQQLLGKFVFDV 187

Query: 197 KHMARFCEGLEGGNLGLEKLGQLLDQKRFGLKHHAGSDSLMTALLHEKM 244
           K +A  C GL     GL+++  +L  KR G  HHAGSDS +TA +  K+
Sbjct: 188 KKIAESCSGL-SSQFGLQRIADVLQMKRVGKAHHAGSDSELTARVFTKL 234

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7027009.11.1e-205100.00putative CCR4-associated factor 1-like 1, partial [Cucurbita argyrosperma subsp.... [more]
KAG6604930.11.9e-20599.71putative CCR4-associated factor 1-like 1, partial [Cucurbita argyrosperma subsp.... [more]
XP_022947868.11.7e-20197.42putative CCR4-associated factor 1 homolog 8 [Cucurbita moschata][more]
XP_023533172.11.8e-20096.85putative CCR4-associated factor 1 homolog 8 [Cucurbita pepo subsp. pepo][more]
XP_022970734.16.8e-17988.70putative CCR4-associated factor 1 homolog 8 [Cucurbita maxima] >XP_022970738.1 p... [more]
Match NameE-valueIdentityDescription
Q9SHJ08.9e-4941.90Probable CCR4-associated factor 1 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
O647731.0e-4442.61Probable CCR4-associated factor 1 homolog 5 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Q9LXM43.0e-4444.75Putative CCR4-associated factor 1 homolog 8 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Q9C6M93.6e-4241.92Putative CCR4-associated factor 1 homolog 4 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Q9SFX66.2e-4241.92Putative CCR4-associated factor 1 homolog 3 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Match NameE-valueIdentityDescription
A0A6J1G7T48.0e-20297.42Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111451626 PE=3... [more]
A0A6J1I6H73.3e-17988.70Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111469633 PE=3 S... [more]
A0A6J1I4S71.2e-17888.41Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111469631 PE=3 S... [more]
A0A6J1I1E63.6e-17888.41putative CCR4-associated factor 1 homolog 8 OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1I3Q42.6e-16890.22Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111469634 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT1G06450.16.3e-5041.90Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT1G61470.17.3e-4642.61Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT3G44240.12.1e-4544.75Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT1G27890.12.6e-4341.92Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT1G27820.14.4e-4341.92Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006941Ribonuclease CAF1PFAMPF04857CAF1coord: 21..131
e-value: 7.8E-6
score: 25.4
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 5..265
e-value: 1.4E-62
score: 213.2
IPR039637CCR4-NOT transcription complex subunit 7/8/Pop2PANTHERPTHR10797CCR4-NOT TRANSCRIPTION COMPLEX SUBUNITcoord: 13..282
NoneNo IPR availablePANTHERPTHR10797:SF57CCR4-ASSOCIATED FACTOR 1 HOMOLOG 3-RELATEDcoord: 13..282
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 15..250

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg05168-RACarg05168-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006402 mRNA catabolic process
biological_process GO:0090503 RNA phosphodiester bond hydrolysis, exonucleolytic
cellular_component GO:0030014 CCR4-NOT complex
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004535 poly(A)-specific ribonuclease activity