Carg05161 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg05161
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	RING-type domain-containing protein
Location	Carg_Chr07: 3246844 .. 3247299 (-)
RNA-Seq Expression	Carg05161
Synteny	Carg05161

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTCTCTCCAGCCTTCCTGCCCCTTCTGAAGGAGTACTAGGAGTGATCCTTGTGAATACGGCAATATCGATTTCCATCTTCAAAGGCATAGTTCGGTCGATCCTCCATGTCGTTGGCATCCATCTTTCGACACCATCATCATCAGATTCCATGGAGAATTCTCCTGAATCCATAGACTTCTGTCTTAATCCAAATGGAGGCTACATTGAAGAGTTTCGTAGTCGGATCCCAGCGATTCTGTTCGACAAAGTTCGTAGCTGCAAATGGCTAGAACACGACTGCTCGGTCTGCCTTACCCAATTTGAACCTGAATCAGAGATTAACCATTTATCTTGTGGCCATGTTTTCCACAGAGTGTGCTTGGAGAAGTGGCTGGACTACTGGAACATTACTTGCCCACTGTGTCGAACTCCATTAATGCCCGAAGAAGAGACAGCCTCCTGCTTCTGGTGA

mRNA sequence

ATGGGTCTCTCCAGCCTTCCTGCCCCTTCTGAAGGAGTACTAGGAGTGATCCTTGTGAATACGGCAATATCGATTTCCATCTTCAAAGGCATAGTTCGGTCGATCCTCCATGTCGTTGGCATCCATCTTTCGACACCATCATCATCAGATTCCATGGAGAATTCTCCTGAATCCATAGACTTCTGTCTTAATCCAAATGGAGGCTACATTGAAGAGTTTCGTAGTCGGATCCCAGCGATTCTGTTCGACAAAGTTCGTAGCTGCAAATGGCTAGAACACGACTGCTCGGTCTGCCTTACCCAATTTGAACCTGAATCAGAGATTAACCATTTATCTTGTGGCCATGTTTTCCACAGAGTGTGCTTGGAGAAGTGGCTGGACTACTGGAACATTACTTGCCCACTGTGTCGAACTCCATTAATGCCCGAAGAAGAGACAGCCTCCTGCTTCTGGTGA

Coding sequence (CDS)

ATGGGTCTCTCCAGCCTTCCTGCCCCTTCTGAAGGAGTACTAGGAGTGATCCTTGTGAATACGGCAATATCGATTTCCATCTTCAAAGGCATAGTTCGGTCGATCCTCCATGTCGTTGGCATCCATCTTTCGACACCATCATCATCAGATTCCATGGAGAATTCTCCTGAATCCATAGACTTCTGTCTTAATCCAAATGGAGGCTACATTGAAGAGTTTCGTAGTCGGATCCCAGCGATTCTGTTCGACAAAGTTCGTAGCTGCAAATGGCTAGAACACGACTGCTCGGTCTGCCTTACCCAATTTGAACCTGAATCAGAGATTAACCATTTATCTTGTGGCCATGTTTTCCACAGAGTGTGCTTGGAGAAGTGGCTGGACTACTGGAACATTACTTGCCCACTGTGTCGAACTCCATTAATGCCCGAAGAAGAGACAGCCTCCTGCTTCTGGTGA

Protein sequence

MGLSSLPAPSEGVLGVILVNTAISISIFKGIVRSILHVVGIHLSTPSSSDSMENSPESIDFCLNPNGGYIEEFRSRIPAILFDKVRSCKWLEHDCSVCLTQFEPESEINHLSCGHVFHRVCLEKWLDYWNITCPLCRTPLMPEEETASCFW

Homology

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BLAST of Carg05161 vs. NCBI nr
Match: XP_022947327.1 (probable E3 ubiquitin-protein ligase XERICO [Cucurbita moschata] >XP_022947328.1 probable E3 ubiquitin-protein ligase XERICO [Cucurbita moschata] >KAG6604923.1 putative E3 ubiquitin-protein ligase XERICO, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027002.1 putative E3 ubiquitin-protein ligase XERICO, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 321.6 bits (823), Expect = 3.6e-84
Identity = 151/151 (100.00%), Postives = 151/151 (100.00%), Query Frame = 0


Query: 1   MGLSSLPAPSEGVLGVILVNTAISISIFKGIVRSILHVVGIHLSTPSSSDSMENSPESID 60
           MGLSSLPAPSEGVLGVILVNTAISISIFKGIVRSILHVVGIHLSTPSSSDSMENSPESID
Sbjct: 1   MGLSSLPAPSEGVLGVILVNTAISISIFKGIVRSILHVVGIHLSTPSSSDSMENSPESID 60

Query: 61  FCLNPNGGYIEEFRSRIPAILFDKVRSCKWLEHDCSVCLTQFEPESEINHLSCGHVFHRV 120
           FCLNPNGGYIEEFRSRIPAILFDKVRSCKWLEHDCSVCLTQFEPESEINHLSCGHVFHRV
Sbjct: 61  FCLNPNGGYIEEFRSRIPAILFDKVRSCKWLEHDCSVCLTQFEPESEINHLSCGHVFHRV 120

Query: 121 CLEKWLDYWNITCPLCRTPLMPEEETASCFW 152
           CLEKWLDYWNITCPLCRTPLMPEEETASCFW
Sbjct: 121 CLEKWLDYWNITCPLCRTPLMPEEETASCFW 151

[Close]

BLAST of Carg05161 vs. NCBI nr
Match: XP_022971123.1 (probable E3 ubiquitin-protein ligase XERICO [Cucurbita maxima] >XP_022971124.1 probable E3 ubiquitin-protein ligase XERICO [Cucurbita maxima])

HSP 1 Score: 321.2 bits (822), Expect = 4.6e-84
Identity = 150/151 (99.34%), Postives = 151/151 (100.00%), Query Frame = 0


Query: 1   MGLSSLPAPSEGVLGVILVNTAISISIFKGIVRSILHVVGIHLSTPSSSDSMENSPESID 60
           MGLSSLPAPSEGVLGVILVNTAISISIFKGIVRSILHVVGIHLSTPSSSDSMENSPESID
Sbjct: 1   MGLSSLPAPSEGVLGVILVNTAISISIFKGIVRSILHVVGIHLSTPSSSDSMENSPESID 60

Query: 61  FCLNPNGGYIEEFRSRIPAILFDKVRSCKWLEHDCSVCLTQFEPESEINHLSCGHVFHRV 120
           FCLNPNGGYIEEFRSRIPAILFDKVRSCKWLEHDCSVCLTQFEPESEINHLSCGHVFHRV
Sbjct: 61  FCLNPNGGYIEEFRSRIPAILFDKVRSCKWLEHDCSVCLTQFEPESEINHLSCGHVFHRV 120

Query: 121 CLEKWLDYWNITCPLCRTPLMPEEETASCFW 152
           CLEKWLDYWN+TCPLCRTPLMPEEETASCFW
Sbjct: 121 CLEKWLDYWNVTCPLCRTPLMPEEETASCFW 151

[Close]

BLAST of Carg05161 vs. NCBI nr
Match: XP_023534541.1 (probable E3 ubiquitin-protein ligase XERICO [Cucurbita pepo subsp. pepo] >XP_023534542.1 probable E3 ubiquitin-protein ligase XERICO [Cucurbita pepo subsp. pepo])

HSP 1 Score: 319.3 bits (817), Expect = 1.8e-83
Identity = 149/151 (98.68%), Postives = 150/151 (99.34%), Query Frame = 0


Query: 1   MGLSSLPAPSEGVLGVILVNTAISISIFKGIVRSILHVVGIHLSTPSSSDSMENSPESID 60
           MGLSSLPAPSEGVLGVILVNTAISISIFKGIVRSILHVVGIHLS PSSSDSMENSPESID
Sbjct: 1   MGLSSLPAPSEGVLGVILVNTAISISIFKGIVRSILHVVGIHLSAPSSSDSMENSPESID 60

Query: 61  FCLNPNGGYIEEFRSRIPAILFDKVRSCKWLEHDCSVCLTQFEPESEINHLSCGHVFHRV 120
           FCLNPNGGYIEEFRSRIPAILFDKVRSCKWLEHDCSVCLTQFEPESEINHLSCGHVFHRV
Sbjct: 61  FCLNPNGGYIEEFRSRIPAILFDKVRSCKWLEHDCSVCLTQFEPESEINHLSCGHVFHRV 120

Query: 121 CLEKWLDYWNITCPLCRTPLMPEEETASCFW 152
           CLEKWLDYWN+TCPLCRTPLMPEEETASCFW
Sbjct: 121 CLEKWLDYWNVTCPLCRTPLMPEEETASCFW 151

[Close]

BLAST of Carg05161 vs. NCBI nr
Match: XP_004139989.1 (probable E3 ubiquitin-protein ligase XERICO [Cucumis sativus] >XP_031742532.1 probable E3 ubiquitin-protein ligase XERICO [Cucumis sativus] >XP_031742533.1 probable E3 ubiquitin-protein ligase XERICO [Cucumis sativus] >KGN46618.1 hypothetical protein Csa_005123 [Cucumis sativus])

HSP 1 Score: 298.9 bits (764), Expect = 2.5e-77
Identity = 141/155 (90.97%), Postives = 148/155 (95.48%), Query Frame = 0


Query: 1   MGLSSLPAPSEGVLGVILVNTAISISIFKGIVRSILHVVGIHLST----PSSSDSMENSP 60
           MGLSSLPAPSEGVLGVILVNTA+SISIFKGIVRSILHVVGIHLS+    PSS DSMEN+P
Sbjct: 1   MGLSSLPAPSEGVLGVILVNTALSISIFKGIVRSILHVVGIHLSSSPTLPSSPDSMENAP 60

Query: 61  ESIDFCLNPNGGYIEEFRSRIPAILFDKVRSCKWLEHDCSVCLTQFEPESEINHLSCGHV 120
           ESI+F LNPNG YIEEFRSRIPAILFDKVRSCKWLEHDCSVCLTQFEPESEINHLSCGH+
Sbjct: 61  ESIEFHLNPNGSYIEEFRSRIPAILFDKVRSCKWLEHDCSVCLTQFEPESEINHLSCGHL 120

Query: 121 FHRVCLEKWLDYWNITCPLCRTPLMPEEETASCFW 152
           FHRVCLEKWLDYWN+TCPLCRTPLMPEE+TASCFW
Sbjct: 121 FHRVCLEKWLDYWNLTCPLCRTPLMPEEDTASCFW 155

[Close]

BLAST of Carg05161 vs. NCBI nr
Match: XP_008448128.1 (PREDICTED: probable E3 ubiquitin-protein ligase XERICO [Cucumis melo] >KAA0060845.1 putative E3 ubiquitin-protein ligase XERICO [Cucumis melo var. makuwa] >TYK27107.1 putative E3 ubiquitin-protein ligase XERICO [Cucumis melo var. makuwa])

HSP 1 Score: 296.2 bits (757), Expect = 1.6e-76
Identity = 140/156 (89.74%), Postives = 147/156 (94.23%), Query Frame = 0


Query: 1   MGLSSLPAPSEGVLGVILVNTAISISIFKGIVRSILHVVGIHLST-----PSSSDSMENS 60
           MGLSSLPAPSEGVLGVILVNTA+SISIFKGIVRSILHVVGIHLS+     PSS DSMEN+
Sbjct: 1   MGLSSLPAPSEGVLGVILVNTALSISIFKGIVRSILHVVGIHLSSSSATLPSSPDSMENA 60

Query: 61  PESIDFCLNPNGGYIEEFRSRIPAILFDKVRSCKWLEHDCSVCLTQFEPESEINHLSCGH 120
           PESI+F LNPNG YIEEFRSRIPAILFDKVRSCKWLEHDCSVCLTQFEPESEINHLSCGH
Sbjct: 61  PESIEFHLNPNGSYIEEFRSRIPAILFDKVRSCKWLEHDCSVCLTQFEPESEINHLSCGH 120

Query: 121 VFHRVCLEKWLDYWNITCPLCRTPLMPEEETASCFW 152
           +FH VCLEKWLDYWN+TCPLCRTPLMPEE+TASCFW
Sbjct: 121 LFHSVCLEKWLDYWNLTCPLCRTPLMPEEDTASCFW 156

[Close]

BLAST of Carg05161 vs. ExPASy Swiss-Prot
Match: Q9SI09 (Probable E3 ubiquitin-protein ligase XERICO OS=Arabidopsis thaliana OX=3702 GN=XERICO PE=1 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 4.9e-44
Identity = 94/158 (59.49%), Postives = 115/158 (72.78%), Query Frame = 0


Query: 1   MGLSSLPAPSEGVLGVILVNTAISISIFKGIVRSILHVVGIHLSTPSSSD-----SMENS 60
           MGLSSLP PSEG+L VILVNTA+SISI KGIVRS L +VGI LS  SSS      S ENS
Sbjct: 1   MGLSSLPGPSEGMLCVILVNTALSISIVKGIVRSFLGIVGISLSPSSSSPSSVTVSSENS 60

Query: 61  --PESIDFCLNPNGGYIEEFRSRIPAILFDKVRSC-KWLEHDCSVCLTQFEPESEINHLS 120
              ES DF +     Y+EEFR+R P + F+ +  C K  +++CSVCL++F+ +SEIN L 
Sbjct: 61  STSESFDFRVCQPESYLEEFRNRTPTLRFESLCRCKKQADNECSVCLSKFQGDSEINKLK 120

Query: 121 CGHVFHRVCLEKWLDYWNITCPLCRTPL--MPEEETAS 149
           CGH+FH+ CLEKW+DYWNITCPLCRTPL  +PE+   S
Sbjct: 121 CGHLFHKTCLEKWIDYWNITCPLCRTPLVVVPEDHQLS 158

[Close]

BLAST of Carg05161 vs. ExPASy Swiss-Prot
Match: Q9XF92 (Brassinosteroid-responsive RING protein 1 OS=Arabidopsis thaliana OX=3702 GN=BRH1 PE=2 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 3.1e-14
Identity = 48/139 (34.53%), Postives = 74/139 (53.24%), Query Frame = 0


Query: 10  SEGVLGVILVNTAISISIFKGIVRSILHVVGI--HLSTPSSSDSMENSPESIDFCLNPNG 69
           +E  L  + V T   +   + IV SI   +G+   L    +     + P  I    +P  
Sbjct: 8   TEVFLPKLFVQTLSILGFIRTIVFSIFRFLGLSDFLEMDQTWPDYTSYPTRIPETRSPFS 67

Query: 70  GYIEEFRSRIPAILFDKV-RSCKWLEHDCSVCLTQFEPESEINHL-SCGHVFHRVCLEKW 129
             +   R  +P I F+++  S + L  +C+VCL +FE E EI  L +C H+FHR CL++W
Sbjct: 68  ALL--IREILPVIKFEELTNSGEDLPENCAVCLYEFEGEQEIRWLRNCRHIFHRSCLDRW 127

Query: 130 LDYWNITCPLCRTPLMPEE 145
           +D+   TCPLCRTP +P+E
Sbjct: 128 MDHDQKTCPLCRTPFVPDE 144

[Close]

BLAST of Carg05161 vs. ExPASy Swiss-Prot
Match: Q9ZU51 (E3 ubiquitin-protein ligase RHA2B OS=Arabidopsis thaliana OX=3702 GN=RHA2B PE=1 SV=2)

HSP 1 Score: 70.1 bits (170), Expect = 2.4e-11
Identity = 26/49 (53.06%), Postives = 34/49 (69.39%), Query Frame = 0


Query: 94  DCSVCLTQFEPESEINHLSCGHVFHRVCLEKWLDYWNITCPLCRTPLMP 143
           DC VCL++ +   E+  L C HVFH+ CLE WL + N  CPLCR+PL+P
Sbjct: 73  DCIVCLSKLKTGEEVRKLDCRHVFHKQCLEGWLQHLNFNCPLCRSPLLP 121

[Close]

BLAST of Carg05161 vs. ExPASy Swiss-Prot
Match: Q9SUS4 (Probable E3 ubiquitin-protein ligase RHA1A OS=Arabidopsis thaliana OX=3702 GN=RHA1A PE=2 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 7.1e-11
Identity = 41/146 (28.08%), Postives = 70/146 (47.95%), Query Frame = 0


Query: 9   PSEGVLGVILVNTAISISIFKGIVRSILHVVGIHLSTPSSSDSMENS-PESIDFCLNPNG 68
           P + +  + + +  + I    G  R I+  +  ++  P   D  E S P+     L+ + 
Sbjct: 4   PEDFITELQIPSYILKILYVIGFFRDIVDALCPYIGLPRFLDHNETSAPDLTRHALSTSA 63

Query: 69  GYIEEFRSRIPAILFDKVRSCKWLEHDCSVCLTQFEPESEINHL-SCGHVFHRVCLEKWL 128
               E    IP + F  + +    E  C+VCL+ FE + ++  L  CGHVFH  CL++W+
Sbjct: 64  SLANEL---IPVVRFSDLPTDP--EDCCTVCLSDFESDDKVRQLPKCGHVFHHYCLDRWI 123

Query: 129 -DYWNITCPLCRTPLMPEEETASCFW 152
            DY  + CP+CR   +P+E+     W
Sbjct: 124 VDYNKMKCPVCRHRFLPKEKYTQSDW 144

[Close]

BLAST of Carg05161 vs. ExPASy Swiss-Prot
Match: Q9SUS5 (E3 ubiquitin-protein ligase RHA1B OS=Arabidopsis thaliana OX=3702 GN=RHA1B PE=2 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.2e-10
Identity = 43/137 (31.39%), Postives = 68/137 (49.64%), Query Frame = 0


Query: 18  LVNTAISISIFKGIVRSILHVVGIHLSTPSSSDSMENS-PESIDFCLNPNGGYIEEFRSR 77
           ++ T   I  F+ +V ++   +G+    PS  D  E S  +     L+ +     E    
Sbjct: 16  VLKTLYVIGFFRDMVDALCPYIGL----PSFLDHNETSRSDPTRLALSTSATLANEL--- 75

Query: 78  IPAILFDKVRSCKWLEHDCSVCLTQFEPESEINHL-SCGHVFHRVCLEKWL-DYWNITCP 137
           IP + F  + +    E  C+VCL+ F  + +I  L  CGHVFH  CL++W+ D   ITCP
Sbjct: 76  IPVVRFSDLLTDP--EDCCTVCLSDFVSDDKIRQLPKCGHVFHHRCLDRWIVDCNKITCP 135

Query: 138 LCRTPLMPEEETASCFW 152
           +CR   +PEE++    W
Sbjct: 136 ICRNRFLPEEKSTPFDW 143

[Close]

BLAST of Carg05161 vs. ExPASy TrEMBL
Match: A0A6J1G653 (probable E3 ubiquitin-protein ligase XERICO OS=Cucurbita moschata OX=3662 GN=LOC111451222 PE=4 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 1.7e-84
Identity = 151/151 (100.00%), Postives = 151/151 (100.00%), Query Frame = 0


Query: 1   MGLSSLPAPSEGVLGVILVNTAISISIFKGIVRSILHVVGIHLSTPSSSDSMENSPESID 60
           MGLSSLPAPSEGVLGVILVNTAISISIFKGIVRSILHVVGIHLSTPSSSDSMENSPESID
Sbjct: 1   MGLSSLPAPSEGVLGVILVNTAISISIFKGIVRSILHVVGIHLSTPSSSDSMENSPESID 60

Query: 61  FCLNPNGGYIEEFRSRIPAILFDKVRSCKWLEHDCSVCLTQFEPESEINHLSCGHVFHRV 120
           FCLNPNGGYIEEFRSRIPAILFDKVRSCKWLEHDCSVCLTQFEPESEINHLSCGHVFHRV
Sbjct: 61  FCLNPNGGYIEEFRSRIPAILFDKVRSCKWLEHDCSVCLTQFEPESEINHLSCGHVFHRV 120

Query: 121 CLEKWLDYWNITCPLCRTPLMPEEETASCFW 152
           CLEKWLDYWNITCPLCRTPLMPEEETASCFW
Sbjct: 121 CLEKWLDYWNITCPLCRTPLMPEEETASCFW 151

[Close]

BLAST of Carg05161 vs. ExPASy TrEMBL
Match: A0A6J1I7P2 (probable E3 ubiquitin-protein ligase XERICO OS=Cucurbita maxima OX=3661 GN=LOC111469890 PE=4 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 2.2e-84
Identity = 150/151 (99.34%), Postives = 151/151 (100.00%), Query Frame = 0


Query: 1   MGLSSLPAPSEGVLGVILVNTAISISIFKGIVRSILHVVGIHLSTPSSSDSMENSPESID 60
           MGLSSLPAPSEGVLGVILVNTAISISIFKGIVRSILHVVGIHLSTPSSSDSMENSPESID
Sbjct: 1   MGLSSLPAPSEGVLGVILVNTAISISIFKGIVRSILHVVGIHLSTPSSSDSMENSPESID 60

Query: 61  FCLNPNGGYIEEFRSRIPAILFDKVRSCKWLEHDCSVCLTQFEPESEINHLSCGHVFHRV 120
           FCLNPNGGYIEEFRSRIPAILFDKVRSCKWLEHDCSVCLTQFEPESEINHLSCGHVFHRV
Sbjct: 61  FCLNPNGGYIEEFRSRIPAILFDKVRSCKWLEHDCSVCLTQFEPESEINHLSCGHVFHRV 120

Query: 121 CLEKWLDYWNITCPLCRTPLMPEEETASCFW 152
           CLEKWLDYWN+TCPLCRTPLMPEEETASCFW
Sbjct: 121 CLEKWLDYWNVTCPLCRTPLMPEEETASCFW 151

[Close]

BLAST of Carg05161 vs. ExPASy TrEMBL
Match: A0A0A0KAU4 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G113550 PE=4 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 1.2e-77
Identity = 141/155 (90.97%), Postives = 148/155 (95.48%), Query Frame = 0


Query: 1   MGLSSLPAPSEGVLGVILVNTAISISIFKGIVRSILHVVGIHLST----PSSSDSMENSP 60
           MGLSSLPAPSEGVLGVILVNTA+SISIFKGIVRSILHVVGIHLS+    PSS DSMEN+P
Sbjct: 1   MGLSSLPAPSEGVLGVILVNTALSISIFKGIVRSILHVVGIHLSSSPTLPSSPDSMENAP 60

Query: 61  ESIDFCLNPNGGYIEEFRSRIPAILFDKVRSCKWLEHDCSVCLTQFEPESEINHLSCGHV 120
           ESI+F LNPNG YIEEFRSRIPAILFDKVRSCKWLEHDCSVCLTQFEPESEINHLSCGH+
Sbjct: 61  ESIEFHLNPNGSYIEEFRSRIPAILFDKVRSCKWLEHDCSVCLTQFEPESEINHLSCGHL 120

Query: 121 FHRVCLEKWLDYWNITCPLCRTPLMPEEETASCFW 152
           FHRVCLEKWLDYWN+TCPLCRTPLMPEE+TASCFW
Sbjct: 121 FHRVCLEKWLDYWNLTCPLCRTPLMPEEDTASCFW 155

[Close]

BLAST of Carg05161 vs. ExPASy TrEMBL
Match: A0A5D3DTM0 (Putative E3 ubiquitin-protein ligase XERICO OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2527G00040 PE=4 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 7.7e-77
Identity = 140/156 (89.74%), Postives = 147/156 (94.23%), Query Frame = 0


Query: 1   MGLSSLPAPSEGVLGVILVNTAISISIFKGIVRSILHVVGIHLST-----PSSSDSMENS 60
           MGLSSLPAPSEGVLGVILVNTA+SISIFKGIVRSILHVVGIHLS+     PSS DSMEN+
Sbjct: 1   MGLSSLPAPSEGVLGVILVNTALSISIFKGIVRSILHVVGIHLSSSSATLPSSPDSMENA 60

Query: 61  PESIDFCLNPNGGYIEEFRSRIPAILFDKVRSCKWLEHDCSVCLTQFEPESEINHLSCGH 120
           PESI+F LNPNG YIEEFRSRIPAILFDKVRSCKWLEHDCSVCLTQFEPESEINHLSCGH
Sbjct: 61  PESIEFHLNPNGSYIEEFRSRIPAILFDKVRSCKWLEHDCSVCLTQFEPESEINHLSCGH 120

Query: 121 VFHRVCLEKWLDYWNITCPLCRTPLMPEEETASCFW 152
           +FH VCLEKWLDYWN+TCPLCRTPLMPEE+TASCFW
Sbjct: 121 LFHSVCLEKWLDYWNLTCPLCRTPLMPEEDTASCFW 156

[Close]

BLAST of Carg05161 vs. ExPASy TrEMBL
Match: A0A1S3BIY9 (probable E3 ubiquitin-protein ligase XERICO OS=Cucumis melo OX=3656 GN=LOC103490418 PE=4 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 7.7e-77
Identity = 140/156 (89.74%), Postives = 147/156 (94.23%), Query Frame = 0


Query: 1   MGLSSLPAPSEGVLGVILVNTAISISIFKGIVRSILHVVGIHLST-----PSSSDSMENS 60
           MGLSSLPAPSEGVLGVILVNTA+SISIFKGIVRSILHVVGIHLS+     PSS DSMEN+
Sbjct: 1   MGLSSLPAPSEGVLGVILVNTALSISIFKGIVRSILHVVGIHLSSSSATLPSSPDSMENA 60

Query: 61  PESIDFCLNPNGGYIEEFRSRIPAILFDKVRSCKWLEHDCSVCLTQFEPESEINHLSCGH 120
           PESI+F LNPNG YIEEFRSRIPAILFDKVRSCKWLEHDCSVCLTQFEPESEINHLSCGH
Sbjct: 61  PESIEFHLNPNGSYIEEFRSRIPAILFDKVRSCKWLEHDCSVCLTQFEPESEINHLSCGH 120

Query: 121 VFHRVCLEKWLDYWNITCPLCRTPLMPEEETASCFW 152
           +FH VCLEKWLDYWN+TCPLCRTPLMPEE+TASCFW
Sbjct: 121 LFHSVCLEKWLDYWNLTCPLCRTPLMPEEDTASCFW 156

[Close]

BLAST of Carg05161 vs. TAIR 10
Match: AT2G04240.1 (RING/U-box superfamily protein )

HSP 1 Score: 178.7 bits (452), Expect = 3.5e-45
Identity = 94/158 (59.49%), Postives = 115/158 (72.78%), Query Frame = 0


Query: 1   MGLSSLPAPSEGVLGVILVNTAISISIFKGIVRSILHVVGIHLSTPSSSD-----SMENS 60
           MGLSSLP PSEG+L VILVNTA+SISI KGIVRS L +VGI LS  SSS      S ENS
Sbjct: 1   MGLSSLPGPSEGMLCVILVNTALSISIVKGIVRSFLGIVGISLSPSSSSPSSVTVSSENS 60

Query: 61  --PESIDFCLNPNGGYIEEFRSRIPAILFDKVRSC-KWLEHDCSVCLTQFEPESEINHLS 120
              ES DF +     Y+EEFR+R P + F+ +  C K  +++CSVCL++F+ +SEIN L 
Sbjct: 61  STSESFDFRVCQPESYLEEFRNRTPTLRFESLCRCKKQADNECSVCLSKFQGDSEINKLK 120

Query: 121 CGHVFHRVCLEKWLDYWNITCPLCRTPL--MPEEETAS 149
           CGH+FH+ CLEKW+DYWNITCPLCRTPL  +PE+   S
Sbjct: 121 CGHLFHKTCLEKWIDYWNITCPLCRTPLVVVPEDHQLS 158

[Close]

BLAST of Carg05161 vs. TAIR 10
Match: AT2G04240.2 (RING/U-box superfamily protein )

HSP 1 Score: 178.7 bits (452), Expect = 3.5e-45
Identity = 94/158 (59.49%), Postives = 115/158 (72.78%), Query Frame = 0


Query: 1   MGLSSLPAPSEGVLGVILVNTAISISIFKGIVRSILHVVGIHLSTPSSSD-----SMENS 60
           MGLSSLP PSEG+L VILVNTA+SISI KGIVRS L +VGI LS  SSS      S ENS
Sbjct: 1   MGLSSLPGPSEGMLCVILVNTALSISIVKGIVRSFLGIVGISLSPSSSSPSSVTVSSENS 60

Query: 61  --PESIDFCLNPNGGYIEEFRSRIPAILFDKVRSC-KWLEHDCSVCLTQFEPESEINHLS 120
              ES DF +     Y+EEFR+R P + F+ +  C K  +++CSVCL++F+ +SEIN L 
Sbjct: 61  STSESFDFRVCQPESYLEEFRNRTPTLRFESLCRCKKQADNECSVCLSKFQGDSEINKLK 120

Query: 121 CGHVFHRVCLEKWLDYWNITCPLCRTPL--MPEEETAS 149
           CGH+FH+ CLEKW+DYWNITCPLCRTPL  +PE+   S
Sbjct: 121 CGHLFHKTCLEKWIDYWNITCPLCRTPLVVVPEDHQLS 158

[Close]

BLAST of Carg05161 vs. TAIR 10
Match: AT1G67856.1 (RING/U-box superfamily protein )

HSP 1 Score: 82.8 bits (203), Expect = 2.6e-16
Identity = 47/146 (32.19%), Postives = 78/146 (53.42%), Query Frame = 0


Query: 1   MGLSSLPAPSEGVLGVILVNTAISISIFKGIVRSILHVVGIHLSTPSSSDSMENSPESID 60
           MG+S  P  SEGV+ ++++NT +S+S+ K +VRS++++V    +   + +  ++  +S  
Sbjct: 1   MGISHYPTASEGVMPMLVMNTVVSVSLVKNMVRSVVNMVSSETNEARNKEDDQDHEDS-- 60

Query: 61  FCLNPNGGYIEEFRSRIPAILFDKVRSCKWLEH-----DCSVCLTQFEPESEINHL-SCG 120
                      + R RI    F+ +   +   +     DC VCL  F+ E E++ L SC 
Sbjct: 61  -----------KRRRRISITHFESLCENRGSRNEREAMDCCVCLCGFKEEEEVSELVSCK 120

Query: 121 HVFHRVCLEKWLDYWNITCPLCRTPL 141
           H FH  CL+KW    + TCPLCR+ L
Sbjct: 121 HYFHSACLDKWFGNNHTTCPLCRSIL 133

[Close]

BLAST of Carg05161 vs. TAIR 10
Match: AT3G61460.1 (brassinosteroid-responsive RING-H2 )

HSP 1 Score: 79.7 bits (195), Expect = 2.2e-15
Identity = 48/139 (34.53%), Postives = 74/139 (53.24%), Query Frame = 0


Query: 10  SEGVLGVILVNTAISISIFKGIVRSILHVVGI--HLSTPSSSDSMENSPESIDFCLNPNG 69
           +E  L  + V T   +   + IV SI   +G+   L    +     + P  I    +P  
Sbjct: 8   TEVFLPKLFVQTLSILGFIRTIVFSIFRFLGLSDFLEMDQTWPDYTSYPTRIPETRSPFS 67

Query: 70  GYIEEFRSRIPAILFDKV-RSCKWLEHDCSVCLTQFEPESEINHL-SCGHVFHRVCLEKW 129
             +   R  +P I F+++  S + L  +C+VCL +FE E EI  L +C H+FHR CL++W
Sbjct: 68  ALL--IREILPVIKFEELTNSGEDLPENCAVCLYEFEGEQEIRWLRNCRHIFHRSCLDRW 127

Query: 130 LDYWNITCPLCRTPLMPEE 145
           +D+   TCPLCRTP +P+E
Sbjct: 128 MDHDQKTCPLCRTPFVPDE 144

[Close]

BLAST of Carg05161 vs. TAIR 10
Match: AT3G43430.1 (RING/U-box superfamily protein )

HSP 1 Score: 71.6 bits (174), Expect = 6.0e-13
Identity = 48/139 (34.53%), Postives = 67/139 (48.20%), Query Frame = 0


Query: 13  VLGVILVNTAISISIFKGIVRSILHVVGIHLSTPSSSDSMENSPESIDFCLNPNGGYIEE 72
           ++  +L  TA+ I++ + I   IL        + SSS S  +S  SI          +  
Sbjct: 11  IISHVLYKTALIITVLRWIFAWILR---YRSRSSSSSSSQSSSSPSISSQTIKESLAVSA 70

Query: 73  FRSRIPAILFDKVRSCKWLEHDCSVCLTQFEPESEINHL-SCGHVFHRVCLEKWLDYW-- 132
           FR  +        RS   +   C+VCL   E E EI  L +C HVFHR C+++WLDY   
Sbjct: 71  FRDAVE-------RSPAAINDMCAVCLGDLEDEDEIRELRNCTHVFHRDCIDRWLDYECC 130

Query: 133 ------NITCPLCRTPLMP 143
                 + TCPLCRTPL+P
Sbjct: 131 GGDDDNHRTCPLCRTPLLP 139

The following BLAST results are available for this feature:

BLAST of Carg05161 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022947327.1	3.6e-84	100.00	probable E3 ubiquitin-protein ligase XERICO [Cucurbita moschata] >XP_022947328.1...	[more]
XP_022971123.1	4.6e-84	99.34	probable E3 ubiquitin-protein ligase XERICO [Cucurbita maxima] >XP_022971124.1 p...	[more]
XP_023534541.1	1.8e-83	98.68	probable E3 ubiquitin-protein ligase XERICO [Cucurbita pepo subsp. pepo] >XP_023...	[more]
XP_004139989.1	2.5e-77	90.97	probable E3 ubiquitin-protein ligase XERICO [Cucumis sativus] >XP_031742532.1 pr...	[more]
XP_008448128.1	1.6e-76	89.74	PREDICTED: probable E3 ubiquitin-protein ligase XERICO [Cucumis melo] >KAA006084...	[more]

BLAST of Carg05161 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SI09	4.9e-44	59.49	Probable E3 ubiquitin-protein ligase XERICO OS=Arabidopsis thaliana OX=3702 GN=X...	[more]
Q9XF92	3.1e-14	34.53	Brassinosteroid-responsive RING protein 1 OS=Arabidopsis thaliana OX=3702 GN=BRH...	[more]
Q9ZU51	2.4e-11	53.06	E3 ubiquitin-protein ligase RHA2B OS=Arabidopsis thaliana OX=3702 GN=RHA2B PE=1 ...	[more]
Q9SUS4	7.1e-11	28.08	Probable E3 ubiquitin-protein ligase RHA1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q9SUS5	1.2e-10	31.39	E3 ubiquitin-protein ligase RHA1B OS=Arabidopsis thaliana OX=3702 GN=RHA1B PE=2 ...	[more]

BLAST of Carg05161 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G653	1.7e-84	100.00	probable E3 ubiquitin-protein ligase XERICO OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1I7P2	2.2e-84	99.34	probable E3 ubiquitin-protein ligase XERICO OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A0A0KAU4	1.2e-77	90.97	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G113550 P...	[more]
A0A5D3DTM0	7.7e-77	89.74	Putative E3 ubiquitin-protein ligase XERICO OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A1S3BIY9	7.7e-77	89.74	probable E3 ubiquitin-protein ligase XERICO OS=Cucumis melo OX=3656 GN=LOC103490...	[more]

BLAST of Carg05161 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G04240.1	3.5e-45	59.49	RING/U-box superfamily protein	[more]
AT2G04240.2	3.5e-45	59.49	RING/U-box superfamily protein	[more]
AT1G67856.1	2.6e-16	32.19	RING/U-box superfamily protein	[more]
AT3G61460.1	2.2e-15	34.53	brassinosteroid-responsive RING-H2	[more]
AT3G43430.1	6.0e-13	34.53	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 95..136 e-value: 6.2E-6 score: 35.7
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 94..137 e-value: 2.6E-11 score: 43.7
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 95..137 score: 12.902362
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 65..142 e-value: 1.2E-16 score: 62.1
None	No IPR available	PANTHER	PTHR47258:SF1	E3 UBIQUITIN-PROTEIN LIGASE XERICO-RELATED	coord: 1..151
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 84..143
IPR044249	Probable E3 ubiquitin-protein ligase XERICO-like	PANTHER	PTHR47258	FAMILY NOT NAMED	coord: 1..151

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg05161-RA	Carg05161-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0008270	zinc ion binding