Carg05146 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg05146
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	glucan endo-1,3-beta-glucosidase 1
Location	Carg_Chr07: 3151607 .. 3152237 (-)
RNA-Seq Expression	Carg05146
Synteny	Carg05146

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGCTCTGTGGACAGGTACCCTGCTCACTCTGGCTCTGCTGTTTCTGTCAACAATTCAGCATAAAGCAGGTAACCACCAATACCCATTTGAAATTTCAAGTCAATAAATGCTTAGTATTATGAAACTTACTTCCAGACAATGAACTCTGAGTAGGTGGGGAGTTTGAGCAATGGTGTATAGCAGACGAGCAGACCCCAGATGATGAACTACAAATGGCTTTGGATTGGGCTTGTGGAAGAGGAGGCGGCAACTGCAGCAAAATCCAACCAAACCAGCCTTGTTTTAACCCAAATACAGTGAAAGATCATGCTTCATTTGCCTTCAATGCTTACTTTCAGAACTTCAAGCATCAGGGTGGATCCTGTTTCTTCAAAGGAGCTGCCATCATTACAGAGCTGGATCCTAGTAAGCCTCACTTTCACTTTCTACTATCTTTATCACTCTTTCTTTCTGGTAAATTGCAAAAGTCACCATGGCCATGCTTCTTCGAGGAGTTGCCAGATCTTGGAGAGGAGAACGAAACCTTCCTTATAAGGGTGTGGAGACCTCTCTCTAACAGATATGTAACGGGCCAAAGTGGACACTGTTTGCCAGCAGTGAGTTTAAGTTATTACAAATGGTATTAG

mRNA sequence

ATGGCTGCTCTGTGGACAGGTACCCTGCTCACTCTGGCTCTGCTGTTTCTGTCAACAATTCAGCATAAAGCAGGTGGGGAGTTTGAGCAATGGTGTATAGCAGACGAGCAGACCCCAGATGATGAACTACAAATGGCTTTGGATTGGGCTTGTGGAAGAGGAGGCGGCAACTGCAGCAAAATCCAACCAAACCAGCCTTGTTTTAACCCAAATACAGTGAAAGATCATGCTTCATTTGCCTTCAATGCTTACTTTCAGAACTTCAAGCATCAGGGTGGATCCTGTTTCTTCAAAGGAGCTGCCATCATTACAGAGCTGGATCCTAGTAAGCCTCACTTTCACTTTCTACTATCTTTATCACTCTTTCTTTCTGGTAAATTGCAAAAGTCACCATGGCCATGCTTCTTCGAGGAGTTGCCAGATCTTGGAGAGGAGAACGAAACCTTCCTTATAAGGGTGTGGAGACCTCTCTCTAACAGATATGTAACGGGCCAAAGTGGACACTGTTTGCCAGCAGTGAGTTTAAGTTATTACAAATGGTATTAG

Coding sequence (CDS)

ATGGCTGCTCTGTGGACAGGTACCCTGCTCACTCTGGCTCTGCTGTTTCTGTCAACAATTCAGCATAAAGCAGGTGGGGAGTTTGAGCAATGGTGTATAGCAGACGAGCAGACCCCAGATGATGAACTACAAATGGCTTTGGATTGGGCTTGTGGAAGAGGAGGCGGCAACTGCAGCAAAATCCAACCAAACCAGCCTTGTTTTAACCCAAATACAGTGAAAGATCATGCTTCATTTGCCTTCAATGCTTACTTTCAGAACTTCAAGCATCAGGGTGGATCCTGTTTCTTCAAAGGAGCTGCCATCATTACAGAGCTGGATCCTAGTAAGCCTCACTTTCACTTTCTACTATCTTTATCACTCTTTCTTTCTGGTAAATTGCAAAAGTCACCATGGCCATGCTTCTTCGAGGAGTTGCCAGATCTTGGAGAGGAGAACGAAACCTTCCTTATAAGGGTGTGGAGACCTCTCTCTAACAGATATGTAACGGGCCAAAGTGGACACTGTTTGCCAGCAGTGAGTTTAAGTTATTACAAATGGTATTAG

Protein sequence

MAALWTGTLLTLALLFLSTIQHKAGGEFEQWCIADEQTPDDELQMALDWACGRGGGNCSKIQPNQPCFNPNTVKDHASFAFNAYFQNFKHQGGSCFFKGAAIITELDPSKPHFHFLLSLSLFLSGKLQKSPWPCFFEELPDLGEENETFLIRVWRPLSNRYVTGQSGHCLPAVSLSYYKWY

Homology

[Close]

BLAST of Carg05146 vs. NCBI nr
Match: KAG7026987.1 (Glucan endo-1,3-beta-glucosidase 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 393.7 bits (1010), Expect = 8.8e-106
Identity = 181/181 (100.00%), Postives = 181/181 (100.00%), Query Frame = 0


Query: 1   MAALWTGTLLTLALLFLSTIQHKAGGEFEQWCIADEQTPDDELQMALDWACGRGGGNCSK 60
           MAALWTGTLLTLALLFLSTIQHKAGGEFEQWCIADEQTPDDELQMALDWACGRGGGNCSK
Sbjct: 1   MAALWTGTLLTLALLFLSTIQHKAGGEFEQWCIADEQTPDDELQMALDWACGRGGGNCSK 60

Query: 61  IQPNQPCFNPNTVKDHASFAFNAYFQNFKHQGGSCFFKGAAIITELDPSKPHFHFLLSLS 120
           IQPNQPCFNPNTVKDHASFAFNAYFQNFKHQGGSCFFKGAAIITELDPSKPHFHFLLSLS
Sbjct: 61  IQPNQPCFNPNTVKDHASFAFNAYFQNFKHQGGSCFFKGAAIITELDPSKPHFHFLLSLS 120

Query: 121 LFLSGKLQKSPWPCFFEELPDLGEENETFLIRVWRPLSNRYVTGQSGHCLPAVSLSYYKW 180
           LFLSGKLQKSPWPCFFEELPDLGEENETFLIRVWRPLSNRYVTGQSGHCLPAVSLSYYKW
Sbjct: 121 LFLSGKLQKSPWPCFFEELPDLGEENETFLIRVWRPLSNRYVTGQSGHCLPAVSLSYYKW 180

Query: 181 Y 182
           Y
Sbjct: 181 Y 181

[Close]

BLAST of Carg05146 vs. NCBI nr
Match: KAG6604905.1 (Glucan endo-1,3-beta-glucosidase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 235.0 bits (598), Expect = 5.2e-58
Identity = 108/108 (100.00%), Postives = 108/108 (100.00%), Query Frame = 0


Query: 1   MAALWTGTLLTLALLFLSTIQHKAGGEFEQWCIADEQTPDDELQMALDWACGRGGGNCSK 60
           MAALWTGTLLTLALLFLSTIQHKAGGEFEQWCIADEQTPDDELQMALDWACGRGGGNCSK
Sbjct: 1   MAALWTGTLLTLALLFLSTIQHKAGGEFEQWCIADEQTPDDELQMALDWACGRGGGNCSK 60

Query: 61  IQPNQPCFNPNTVKDHASFAFNAYFQNFKHQGGSCFFKGAAIITELDP 109
           IQPNQPCFNPNTVKDHASFAFNAYFQNFKHQGGSCFFKGAAIITELDP
Sbjct: 61  IQPNQPCFNPNTVKDHASFAFNAYFQNFKHQGGSCFFKGAAIITELDP 108

[Close]

BLAST of Carg05146 vs. NCBI nr
Match: XP_022947538.1 (glucan endo-1,3-beta-glucosidase 12-like [Cucurbita moschata] >XP_022971134.1 glucan endo-1,3-beta-glucosidase 12 [Cucurbita maxima] >XP_023532863.1 glucan endo-1,3-beta-glucosidase 12-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 234.2 bits (596), Expect = 9.0e-58
Identity = 108/109 (99.08%), Postives = 108/109 (99.08%), Query Frame = 0


Query: 1   MAALWTGTLLTLALLFLSTIQHKAGGEFEQWCIADEQTPDDELQMALDWACGRGGGNCSK 60
           MAALWTGTLLTLALL LSTIQHKAGGEFEQWCIADEQTPDDELQMALDWACGRGGGNCSK
Sbjct: 1   MAALWTGTLLTLALLLLSTIQHKAGGEFEQWCIADEQTPDDELQMALDWACGRGGGNCSK 60

Query: 61  IQPNQPCFNPNTVKDHASFAFNAYFQNFKHQGGSCFFKGAAIITELDPS 110
           IQPNQPCFNPNTVKDHASFAFNAYFQNFKHQGGSCFFKGAAIITELDPS
Sbjct: 61  IQPNQPCFNPNTVKDHASFAFNAYFQNFKHQGGSCFFKGAAIITELDPS 109

[Close]

BLAST of Carg05146 vs. NCBI nr
Match: XP_023532862.1 (glucan endo-1,3-beta-glucosidase 12-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 222.2 bits (565), Expect = 3.5e-54
Identity = 102/103 (99.03%), Postives = 102/103 (99.03%), Query Frame = 0


Query: 7   GTLLTLALLFLSTIQHKAGGEFEQWCIADEQTPDDELQMALDWACGRGGGNCSKIQPNQP 66
           GTLLTLALL LSTIQHKAGGEFEQWCIADEQTPDDELQMALDWACGRGGGNCSKIQPNQP
Sbjct: 15  GTLLTLALLLLSTIQHKAGGEFEQWCIADEQTPDDELQMALDWACGRGGGNCSKIQPNQP 74

Query: 67  CFNPNTVKDHASFAFNAYFQNFKHQGGSCFFKGAAIITELDPS 110
           CFNPNTVKDHASFAFNAYFQNFKHQGGSCFFKGAAIITELDPS
Sbjct: 75  CFNPNTVKDHASFAFNAYFQNFKHQGGSCFFKGAAIITELDPS 117

[Close]

BLAST of Carg05146 vs. NCBI nr
Match: KAA0046037.1 (glucan endo-1,3-beta-glucosidase 12 isoform X1 [Cucumis melo var. makuwa] >TYK11917.1 glucan endo-1,3-beta-glucosidase 12 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 199.5 bits (506), Expect = 2.4e-47
Identity = 94/113 (83.19%), Postives = 97/113 (85.84%), Query Frame = 0


Query: 1   MAALWTGTLLTLALLFLSTIQHKAGGEFEQWCIADEQTPDDELQMALDWACGRGGGNCSK 60
           M AL T TL  L LL LSTI    GGEFEQWC+ADEQ PDDELQMALDWACG+GG NCS 
Sbjct: 1   MVALLTKTL--LILLLLSTIHQNTGGEFEQWCVADEQIPDDELQMALDWACGKGGANCSS 60

Query: 61  IQPNQPCFNPNTVKDHASFAFNAYFQNFKHQGGSCFFKGAAIITELDPSKPHF 114
           IQPNQPCFNPNTVKDHASFAFN YFQ+FKHQGGSCFFKGAAIITELDPSK HF
Sbjct: 61  IQPNQPCFNPNTVKDHASFAFNNYFQSFKHQGGSCFFKGAAIITELDPSKLHF 111

[Close]

BLAST of Carg05146 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 2.4e-21
Identity = 46/91 (50.55%), Postives = 57/91 (62.64%), Query Frame = 0


Query: 19  TIQHKAGGEFEQWCIADEQTPDDELQMALDWACGRGGGNCSKIQPNQPCFNPNTVKDHAS 78
           TI    GG  ++WCIA  Q    ELQ ALDWACG G  +CS +QP+QPCF P+TV  HAS
Sbjct: 379 TIGGGGGGGTKKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQPCFEPDTVLSHAS 438

Query: 79  FAFNAYFQNFKHQGGSCFFKGAAIITELDPS 110
           +AFN Y+Q        C F GA++  + DPS
Sbjct: 439 YAFNTYYQQSGASSIDCSFNGASVEVDKDPS 469

[Close]

BLAST of Carg05146 vs. ExPASy Swiss-Prot
Match: Q94CD8 (Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana OX=3702 GN=At3g13560 PE=2 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 9.1e-21
Identity = 44/79 (55.70%), Postives = 53/79 (67.09%), Query Frame = 0


Query: 31  WCIADEQTPDDELQMALDWACGRGGGNCSKIQPNQPCFNPNTVKDHASFAFNAYFQNFKH 90
           +C+A     DD+L   L+WACG+G  NC+ IQP QPC+ PN VK HASFAFN Y+Q  K 
Sbjct: 362 FCVAKADADDDKLVDGLNWACGQGRANCAAIQPGQPCYLPNDVKSHASFAFNDYYQKMKS 421

Query: 91  QGGSCFFKGAAIITELDPS 110
            GG+C F G AI T  DPS
Sbjct: 422 AGGTCDFDGTAITTTRDPS 440

[Close]

BLAST of Carg05146 vs. ExPASy Swiss-Prot
Match: O65399 (Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 PE=2 SV=3)

HSP 1 Score: 100.5 bits (249), Expect = 2.0e-20
Identity = 45/81 (55.56%), Postives = 54/81 (66.67%), Query Frame = 0


Query: 29  EQWCIADEQTPDDELQMALDWACGRGGGNCSKIQPNQPCFNPNTVKDHASFAFNAYFQNF 88
           + +CIA +      LQ ALDWACG G  NCS+IQP + C+ PN VK HASFAFN+Y+Q  
Sbjct: 379 QTYCIAMDGVDAKTLQAALDWACGPGRSNCSEIQPGESCYQPNNVKGHASFAFNSYYQKE 438

Query: 89  KHQGGSCFFKGAAIITELDPS 110
               GSC FKG A+IT  DPS
Sbjct: 439 GRASGSCDFKGVAMITTTDPS 459

[Close]

BLAST of Carg05146 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 8.5e-19
Identity = 40/86 (46.51%), Postives = 54/86 (62.79%), Query Frame = 0


Query: 24  AGGEFEQWCIADEQTPDDELQMALDWACGRGGGNCSKIQPNQPCFNPNTVKDHASFAFNA 83
           + G    WCIA  +  + +L+ ALDWACG G  +C+ IQP+QPCF P+T+  HASF FN+
Sbjct: 362 SSGSSNSWCIASSKASERDLKGALDWACGPGNVDCTAIQPSQPCFQPDTLVSHASFVFNS 421

Query: 84  YFQNFKHQGGSCFFKGAAIITELDPS 110
           YFQ  +    +C F GA +    DPS
Sbjct: 422 YFQQNRATDVACSFGGAGVKVNKDPS 447

[Close]

BLAST of Carg05146 vs. ExPASy Swiss-Prot
Match: Q9ZU91 (Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 PE=2 SV=2)

HSP 1 Score: 86.3 bits (212), Expect = 4.0e-16
Identity = 37/82 (45.12%), Postives = 51/82 (62.20%), Query Frame = 0


Query: 29  EQWCIADEQTPDDELQMALDWACGRGGGNCSKIQPNQPCFNPNTVKDHASFAFNAYFQNF 88
           + +CIA E+     LQ ALDWACG G  +CS +   + C+ P+ V  H+++AFNAY+Q  
Sbjct: 358 QTFCIAKEKVDRKMLQAALDWACGPGKVDCSALMQGESCYEPDDVVAHSTYAFNAYYQKM 417

Query: 89  KHQGGSCFFKGAAIITELDPSK 111
               GSC FKG A +T  DPS+
Sbjct: 418 GKASGSCDFKGVATVTTTDPSR 439

[Close]

BLAST of Carg05146 vs. ExPASy TrEMBL
Match: A0A6J1I7Q3 (glucan endo-1,3-beta-glucosidase 12 OS=Cucurbita maxima OX=3661 GN=LOC111469899 PE=4 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 4.3e-58
Identity = 108/109 (99.08%), Postives = 108/109 (99.08%), Query Frame = 0


Query: 1   MAALWTGTLLTLALLFLSTIQHKAGGEFEQWCIADEQTPDDELQMALDWACGRGGGNCSK 60
           MAALWTGTLLTLALL LSTIQHKAGGEFEQWCIADEQTPDDELQMALDWACGRGGGNCSK
Sbjct: 1   MAALWTGTLLTLALLLLSTIQHKAGGEFEQWCIADEQTPDDELQMALDWACGRGGGNCSK 60

Query: 61  IQPNQPCFNPNTVKDHASFAFNAYFQNFKHQGGSCFFKGAAIITELDPS 110
           IQPNQPCFNPNTVKDHASFAFNAYFQNFKHQGGSCFFKGAAIITELDPS
Sbjct: 61  IQPNQPCFNPNTVKDHASFAFNAYFQNFKHQGGSCFFKGAAIITELDPS 109

[Close]

BLAST of Carg05146 vs. ExPASy TrEMBL
Match: A0A6J1G6V9 (glucan endo-1,3-beta-glucosidase 12-like OS=Cucurbita moschata OX=3662 GN=LOC111451375 PE=4 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 4.3e-58
Identity = 108/109 (99.08%), Postives = 108/109 (99.08%), Query Frame = 0


Query: 1   MAALWTGTLLTLALLFLSTIQHKAGGEFEQWCIADEQTPDDELQMALDWACGRGGGNCSK 60
           MAALWTGTLLTLALL LSTIQHKAGGEFEQWCIADEQTPDDELQMALDWACGRGGGNCSK
Sbjct: 1   MAALWTGTLLTLALLLLSTIQHKAGGEFEQWCIADEQTPDDELQMALDWACGRGGGNCSK 60

Query: 61  IQPNQPCFNPNTVKDHASFAFNAYFQNFKHQGGSCFFKGAAIITELDPS 110
           IQPNQPCFNPNTVKDHASFAFNAYFQNFKHQGGSCFFKGAAIITELDPS
Sbjct: 61  IQPNQPCFNPNTVKDHASFAFNAYFQNFKHQGGSCFFKGAAIITELDPS 109

[Close]

BLAST of Carg05146 vs. ExPASy TrEMBL
Match: A0A5A7TVX0 (Glucan endo-1,3-beta-glucosidase 12 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold177G00840 PE=4 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 1.2e-47
Identity = 94/113 (83.19%), Postives = 97/113 (85.84%), Query Frame = 0


Query: 1   MAALWTGTLLTLALLFLSTIQHKAGGEFEQWCIADEQTPDDELQMALDWACGRGGGNCSK 60
           M AL T TL  L LL LSTI    GGEFEQWC+ADEQ PDDELQMALDWACG+GG NCS 
Sbjct: 1   MVALLTKTL--LILLLLSTIHQNTGGEFEQWCVADEQIPDDELQMALDWACGKGGANCSS 60

Query: 61  IQPNQPCFNPNTVKDHASFAFNAYFQNFKHQGGSCFFKGAAIITELDPSKPHF 114
           IQPNQPCFNPNTVKDHASFAFN YFQ+FKHQGGSCFFKGAAIITELDPSK HF
Sbjct: 61  IQPNQPCFNPNTVKDHASFAFNNYFQSFKHQGGSCFFKGAAIITELDPSKLHF 111

[Close]

BLAST of Carg05146 vs. ExPASy TrEMBL
Match: A0A0A0KDX6 (X8 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G110840 PE=4 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 5.9e-47
Identity = 93/109 (85.32%), Postives = 95/109 (87.16%), Query Frame = 0


Query: 1   MAALWTGTLLTLALLFLSTIQHKAGGEFEQWCIADEQTPDDELQMALDWACGRGGGNCSK 60
           M AL T TL  L LL LSTI    GGEFEQWC+ADEQTPDDELQMALDWACGRGG NCS 
Sbjct: 1   MVALLTKTL--LVLLLLSTIHQNTGGEFEQWCVADEQTPDDELQMALDWACGRGGANCSS 60

Query: 61  IQPNQPCFNPNTVKDHASFAFNAYFQNFKHQGGSCFFKGAAIITELDPS 110
           IQPNQPCFNPNTVKDHASFAFN YFQ+FKHQGGSCFFKGAAIITELDPS
Sbjct: 61  IQPNQPCFNPNTVKDHASFAFNNYFQSFKHQGGSCFFKGAAIITELDPS 107

[Close]

BLAST of Carg05146 vs. ExPASy TrEMBL
Match: A0A1S3CKX6 (glucan endo-1,3-beta-glucosidase 12 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103502123 PE=4 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 8.5e-46
Identity = 91/109 (83.49%), Postives = 94/109 (86.24%), Query Frame = 0


Query: 1   MAALWTGTLLTLALLFLSTIQHKAGGEFEQWCIADEQTPDDELQMALDWACGRGGGNCSK 60
           M AL T TL  L LL LSTI    GGEFEQWC+ADEQ PDDELQMALDWACG+GG NCS 
Sbjct: 1   MVALLTKTL--LILLLLSTIHQNTGGEFEQWCVADEQIPDDELQMALDWACGKGGANCSS 60

Query: 61  IQPNQPCFNPNTVKDHASFAFNAYFQNFKHQGGSCFFKGAAIITELDPS 110
           IQPNQPCFNPNTVKDHASFAFN YFQ+FKHQGGSCFFKGAAIITELDPS
Sbjct: 61  IQPNQPCFNPNTVKDHASFAFNNYFQSFKHQGGSCFFKGAAIITELDPS 107

[Close]

BLAST of Carg05146 vs. TAIR 10
Match: AT5G35740.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 154.8 bits (390), Expect = 6.4e-38
Identity = 68/102 (66.67%), Postives = 85/102 (83.33%), Query Frame = 0


Query: 9   LLTLALLFLSTIQHKAGGEFEQWCIADEQTPDDELQMALDWACGRGGGNCSKI-QPNQPC 68
           +L   L+   T+  ++  E EQWCIADEQTPDDELQ ALDWACG+GG +CSK+ Q NQPC
Sbjct: 8   ILLPLLIIAMTLPRRSEAESEQWCIADEQTPDDELQAALDWACGKGGADCSKMQQENQPC 67

Query: 69  FNPNTVKDHASFAFNAYFQNFKHQGGSCFFKGAAIITELDPS 110
           F PNT++DHASFAFN+Y+Q +K++GGSC+FKGAA+ITELDPS
Sbjct: 68  FLPNTIRDHASFAFNSYYQTYKNKGGSCYFKGAAMITELDPS 109

[Close]

BLAST of Carg05146 vs. TAIR 10
Match: AT2G04910.2 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 129.8 bits (325), Expect = 2.2e-30
Identity = 56/92 (60.87%), Postives = 64/92 (69.57%), Query Frame = 0


Query: 24  AGGEFEQWCIADEQTPDDELQMALDWACGRGGGNCSKIQPNQPCFNPNTVKDHASFAFNA 83
           A  EF QWC+AD Q PD+ +Q A+DWAC  GG +CS IQPNQPCF PNTVKDHAS  FN 
Sbjct: 8   AKAEFGQWCVADGQIPDNVIQAAVDWACQTGGADCSTIQPNQPCFLPNTVKDHASVVFNN 67

Query: 84  YFQNFKHQGGSCFFKGAAIITELDPSKPHFHF 116
           Y+Q +K  GGSC F   A IT+ DPS    HF
Sbjct: 68  YYQRYKRNGGSCNFNSTAFITQTDPSHDSCHF 99

[Close]

BLAST of Carg05146 vs. TAIR 10
Match: AT2G04910.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 128.6 bits (322), Expect = 4.9e-30
Identity = 55/87 (63.22%), Postives = 63/87 (72.41%), Query Frame = 0


Query: 24  AGGEFEQWCIADEQTPDDELQMALDWACGRGGGNCSKIQPNQPCFNPNTVKDHASFAFNA 83
           A  EF QWC+AD Q PD+ +Q A+DWAC  GG +CS IQPNQPCF PNTVKDHAS  FN 
Sbjct: 8   AKAEFGQWCVADGQIPDNVIQAAVDWACQTGGADCSTIQPNQPCFLPNTVKDHASVVFNN 67

Query: 84  YFQNFKHQGGSCFFKGAAIITELDPSK 111
           Y+Q +K  GGSC F   A IT+ DPSK
Sbjct: 68  YYQRYKRNGGSCNFNSTAFITQTDPSK 94

[Close]

BLAST of Carg05146 vs. TAIR 10
Match: AT4G29360.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 105.5 bits (262), Expect = 4.5e-23
Identity = 47/92 (51.09%), Postives = 58/92 (63.04%), Query Frame = 0


Query: 19  TIQHKAGGEFEQWCIADEQTPDDELQMALDWACGRGGGNCSKIQPNQPCFNPNTVKDHAS 78
           TI    GG  ++WCIA  Q    ELQ ALDWACG G  +CS +QP+QPCF P+TV  HAS
Sbjct: 379 TIGGGGGGGTKKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQPCFEPDTVLSHAS 438

Query: 79  FAFNAYFQNFKHQGGSCFFKGAAIITELDPSK 111
           +AFN Y+Q        C F GA++  + DPSK
Sbjct: 439 YAFNTYYQQSGASSIDCSFNGASVEVDKDPSK 470

[Close]

BLAST of Carg05146 vs. TAIR 10
Match: AT1G29380.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 104.8 bits (260), Expect = 7.6e-23
Identity = 53/103 (51.46%), Postives = 64/103 (62.14%), Query Frame = 0


Query: 30  QWCIADEQTPDDELQMALDWACGRGGGNCSKIQPNQPCFNPNTVKDHASFAFNAYFQNFK 89
           QWCIA        LQ+ALD+ACG GG +C +IQ    C+ PNT++DHASFAFN+Y+Q  K
Sbjct: 147 QWCIAKANASPTSLQVALDYACGYGGADCGQIQQGAACYEPNTIRDHASFAFNSYYQ--K 206

Query: 90  HQGG-SCFFKGAAIITELDPSKPHFHFLLSLSLFLSGKLQKSP 132
           H G  SC F GAA +T  DPSK   HF  S     SG +  SP
Sbjct: 207 HPGSDSCNFGGAAQLTSTDPSKGSCHFSSS-----SGTVSTSP 242

The following BLAST results are available for this feature:

BLAST of Carg05146 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7026987.1	8.8e-106	100.00	Glucan endo-1,3-beta-glucosidase 4, partial [Cucurbita argyrosperma subsp. argyr...	[more]
KAG6604905.1	5.2e-58	100.00	Glucan endo-1,3-beta-glucosidase 1, partial [Cucurbita argyrosperma subsp. soror...	[more]
XP_022947538.1	9.0e-58	99.08	glucan endo-1,3-beta-glucosidase 12-like [Cucurbita moschata] >XP_022971134.1 gl...	[more]
XP_023532862.1	3.5e-54	99.03	glucan endo-1,3-beta-glucosidase 12-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
KAA0046037.1	2.4e-47	83.19	glucan endo-1,3-beta-glucosidase 12 isoform X1 [Cucumis melo var. makuwa] >TYK11...	[more]

BLAST of Carg05146 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VYE5	2.4e-21	50.55	Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360...	[more]
Q94CD8	9.1e-21	55.70	Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana OX=3702 GN=At3g13560 ...	[more]
O65399	2.0e-20	55.56	Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 ...	[more]
Q9FJU9	8.5e-19	46.51	Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590...	[more]
Q9ZU91	4.0e-16	45.12	Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 ...	[more]

BLAST of Carg05146 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1I7Q3	4.3e-58	99.08	glucan endo-1,3-beta-glucosidase 12 OS=Cucurbita maxima OX=3661 GN=LOC111469899 ...	[more]
A0A6J1G6V9	4.3e-58	99.08	glucan endo-1,3-beta-glucosidase 12-like OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A5A7TVX0	1.2e-47	83.19	Glucan endo-1,3-beta-glucosidase 12 isoform X1 OS=Cucumis melo var. makuwa OX=11...	[more]
A0A0A0KDX6	5.9e-47	85.32	X8 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G110840 PE=4 SV=...	[more]
A0A1S3CKX6	8.5e-46	83.49	glucan endo-1,3-beta-glucosidase 12 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103...	[more]

BLAST of Carg05146 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G35740.1	6.4e-38	66.67	Carbohydrate-binding X8 domain superfamily protein	[more]
AT2G04910.2	2.2e-30	60.87	Carbohydrate-binding X8 domain superfamily protein	[more]
AT2G04910.1	4.9e-30	63.22	Carbohydrate-binding X8 domain superfamily protein	[more]
AT4G29360.2	4.5e-23	51.09	O-Glycosyl hydrolases family 17 protein	[more]
AT1G29380.1	7.6e-23	51.46	Carbohydrate-binding X8 domain superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 30..113 e-value: 2.6E-39 score: 146.6
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 30..101 e-value: 2.9E-21 score: 75.9
None	No IPR available	GENE3D	1.20.58.1040		coord: 27..114 e-value: 1.6E-9 score: 40.0
None	No IPR available	PANTHER	PTHR31044:SF35	CARBOHYDRATE-BINDING X8 DOMAIN SUPERFAMILY PROTEIN	coord: 8..115
IPR044788	Carbohydrate-binding X8 domain-containing protein, plant	PANTHER	PTHR31044	BETA-1,3 GLUCANASE	coord: 8..115

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg05146-RA	Carg05146-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0009506	plasmodesma
molecular_function	GO:0016787	hydrolase activity