Carg05117 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg05117
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	ethylene-responsive transcription factor 1B-like
Location	Carg_Chr08: 1688509 .. 1689147 (-)
RNA-Seq Expression	Carg05117
Synteny	Carg05117

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCCTGGATAACCATCTGTTTGGTTACCAAGAAAATTTTGAAAATTTTCCCGGAAAACAGAATTCCCATCTCTCATTTCACTTCAATTACTTGGAAGAGATGTTGTTTGCCGACATGTCGTTATCGTCATCGTCATCGTCGTCTGATTCGAGCCCTTGTTTGTCTCCGGAGATGCACGTTAAGGAGGAGATAGAAGACGGCGATATCATCGTGCAAGACGCTCAGACACAGAAGGAGGAGAGGGCTTATAGAGGGGTGCGGCGGAGGCCGTGGGGGAAGTACGCAGCCGAGATAAGAGACTCGACTCGACAAGGGGTGAGGGTTTGGTTGGGGACGTTTGATAGTGCGGAGGCTGCAGCATTGGCTTATGACCAAGCCGCATTTTGCATGAGAGGTGCATTAGCCACACTCAATTTTCCGGTGGAGGTTGTTCGAGAGTCGCTCTATGAAATGAAGTATCGGTGGGAAGTCGGGTGTTCGCCTGTCTTGGCGCTCAAACGACGTCACACGATACGGAGGAAGAAGATGGCAGAAGCCGAGAAGATGGAGAAGAAGATGAAGAAGAAGAAAAATGTAGTTGTTTTTGAGGATTTGGGTGTCGAGTACTTAGAAGAACTCTTAAGTTTGACTTCTTAA

mRNA sequence

ATGTCCCTGGATAACCATCTGTTTGGTTACCAAGAAAATTTTGAAAATTTTCCCGGAAAACAGAATTCCCATCTCTCATTTCACTTCAATTACTTGGAAGAGATGTTGTTTGCCGACATGTCGTTATCGTCATCGTCATCGTCGTCTGATTCGAGCCCTTGTTTGTCTCCGGAGATGCACGTTAAGGAGGAGATAGAAGACGGCGATATCATCGTGCAAGACGCTCAGACACAGAAGGAGGAGAGGGCTTATAGAGGGGTGCGGCGGAGGCCGTGGGGGAAGTACGCAGCCGAGATAAGAGACTCGACTCGACAAGGGGTGAGGGTTTGGTTGGGGACGTTTGATAGTGCGGAGGCTGCAGCATTGGCTTATGACCAAGCCGCATTTTGCATGAGAGGTGCATTAGCCACACTCAATTTTCCGGTGGAGGTTGTTCGAGAGTCGCTCTATGAAATGAAGTATCGGTGGGAAGTCGGGTGTTCGCCTGTCTTGGCGCTCAAACGACGTCACACGATACGGAGGAAGAAGATGGCAGAAGCCGAGAAGATGGAGAAGAAGATGAAGAAGAAGAAAAATGTAGTTGTTTTTGAGGATTTGGGTGTCGAGTACTTAGAAGAACTCTTAAGTTTGACTTCTTAA

Coding sequence (CDS)

ATGTCCCTGGATAACCATCTGTTTGGTTACCAAGAAAATTTTGAAAATTTTCCCGGAAAACAGAATTCCCATCTCTCATTTCACTTCAATTACTTGGAAGAGATGTTGTTTGCCGACATGTCGTTATCGTCATCGTCATCGTCGTCTGATTCGAGCCCTTGTTTGTCTCCGGAGATGCACGTTAAGGAGGAGATAGAAGACGGCGATATCATCGTGCAAGACGCTCAGACACAGAAGGAGGAGAGGGCTTATAGAGGGGTGCGGCGGAGGCCGTGGGGGAAGTACGCAGCCGAGATAAGAGACTCGACTCGACAAGGGGTGAGGGTTTGGTTGGGGACGTTTGATAGTGCGGAGGCTGCAGCATTGGCTTATGACCAAGCCGCATTTTGCATGAGAGGTGCATTAGCCACACTCAATTTTCCGGTGGAGGTTGTTCGAGAGTCGCTCTATGAAATGAAGTATCGGTGGGAAGTCGGGTGTTCGCCTGTCTTGGCGCTCAAACGACGTCACACGATACGGAGGAAGAAGATGGCAGAAGCCGAGAAGATGGAGAAGAAGATGAAGAAGAAGAAAAATGTAGTTGTTTTTGAGGATTTGGGTGTCGAGTACTTAGAAGAACTCTTAAGTTTGACTTCTTAA

Protein sequence

MSLDNHLFGYQENFENFPGKQNSHLSFHFNYLEEMLFADMSLSSSSSSSDSSPCLSPEMHVKEEIEDGDIIVQDAQTQKEERAYRGVRRRPWGKYAAEIRDSTRQGVRVWLGTFDSAEAAALAYDQAAFCMRGALATLNFPVEVVRESLYEMKYRWEVGCSPVLALKRRHTIRRKKMAEAEKMEKKMKKKKNVVVFEDLGVEYLEELLSLTS

Homology

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BLAST of Carg05117 vs. NCBI nr
Match: KAG7025485.1 (Ethylene-responsive transcription factor 1B, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 412.1 bits (1058), Expect = 2.8e-111
Identity = 212/212 (100.00%), Postives = 212/212 (100.00%), Query Frame = 0


Query: 1   MSLDNHLFGYQENFENFPGKQNSHLSFHFNYLEEMLFADMSLSSSSSSSDSSPCLSPEMH 60
           MSLDNHLFGYQENFENFPGKQNSHLSFHFNYLEEMLFADMSLSSSSSSSDSSPCLSPEMH
Sbjct: 1   MSLDNHLFGYQENFENFPGKQNSHLSFHFNYLEEMLFADMSLSSSSSSSDSSPCLSPEMH 60

Query: 61  VKEEIEDGDIIVQDAQTQKEERAYRGVRRRPWGKYAAEIRDSTRQGVRVWLGTFDSAEAA 120
           VKEEIEDGDIIVQDAQTQKEERAYRGVRRRPWGKYAAEIRDSTRQGVRVWLGTFDSAEAA
Sbjct: 61  VKEEIEDGDIIVQDAQTQKEERAYRGVRRRPWGKYAAEIRDSTRQGVRVWLGTFDSAEAA 120

Query: 121 ALAYDQAAFCMRGALATLNFPVEVVRESLYEMKYRWEVGCSPVLALKRRHTIRRKKMAEA 180
           ALAYDQAAFCMRGALATLNFPVEVVRESLYEMKYRWEVGCSPVLALKRRHTIRRKKMAEA
Sbjct: 121 ALAYDQAAFCMRGALATLNFPVEVVRESLYEMKYRWEVGCSPVLALKRRHTIRRKKMAEA 180

Query: 181 EKMEKKMKKKKNVVVFEDLGVEYLEELLSLTS 213
           EKMEKKMKKKKNVVVFEDLGVEYLEELLSLTS
Sbjct: 181 EKMEKKMKKKKNVVVFEDLGVEYLEELLSLTS 212

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BLAST of Carg05117 vs. NCBI nr
Match: KAG6593075.1 (Ethylene-response factor C3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 409.5 bits (1051), Expect = 1.8e-110
Identity = 211/212 (99.53%), Postives = 211/212 (99.53%), Query Frame = 0


Query: 1   MSLDNHLFGYQENFENFPGKQNSHLSFHFNYLEEMLFADMSLSSSSSSSDSSPCLSPEMH 60
           MSL NHLFGYQENFENFPGKQNSHLSFHFNYLEEMLFADMSLSSSSSSSDSSPCLSPEMH
Sbjct: 1   MSLHNHLFGYQENFENFPGKQNSHLSFHFNYLEEMLFADMSLSSSSSSSDSSPCLSPEMH 60

Query: 61  VKEEIEDGDIIVQDAQTQKEERAYRGVRRRPWGKYAAEIRDSTRQGVRVWLGTFDSAEAA 120
           VKEEIEDGDIIVQDAQTQKEERAYRGVRRRPWGKYAAEIRDSTRQGVRVWLGTFDSAEAA
Sbjct: 61  VKEEIEDGDIIVQDAQTQKEERAYRGVRRRPWGKYAAEIRDSTRQGVRVWLGTFDSAEAA 120

Query: 121 ALAYDQAAFCMRGALATLNFPVEVVRESLYEMKYRWEVGCSPVLALKRRHTIRRKKMAEA 180
           ALAYDQAAFCMRGALATLNFPVEVVRESLYEMKYRWEVGCSPVLALKRRHTIRRKKMAEA
Sbjct: 121 ALAYDQAAFCMRGALATLNFPVEVVRESLYEMKYRWEVGCSPVLALKRRHTIRRKKMAEA 180

Query: 181 EKMEKKMKKKKNVVVFEDLGVEYLEELLSLTS 213
           EKMEKKMKKKKNVVVFEDLGVEYLEELLSLTS
Sbjct: 181 EKMEKKMKKKKNVVVFEDLGVEYLEELLSLTS 212

[Close]

BLAST of Carg05117 vs. NCBI nr
Match: XP_022959670.1 (ethylene-responsive transcription factor 1B-like [Cucurbita moschata])

HSP 1 Score: 401.7 bits (1031), Expect = 3.8e-108
Identity = 209/212 (98.58%), Postives = 209/212 (98.58%), Query Frame = 0


Query: 1   MSLDNHLFGYQENFENFPGKQNSHLSFHFNYLEEMLFADMSLSSSSSSSDSSPCLSPEMH 60
           MSL NHLFGYQENFENFPGKQNSHLSFHFNYLEEMLFADMSL  SSSSSDSSPCLSPEMH
Sbjct: 1   MSLHNHLFGYQENFENFPGKQNSHLSFHFNYLEEMLFADMSL--SSSSSDSSPCLSPEMH 60

Query: 61  VKEEIEDGDIIVQDAQTQKEERAYRGVRRRPWGKYAAEIRDSTRQGVRVWLGTFDSAEAA 120
           VKEEIEDGDIIVQDAQTQKEERAYRGVRRRPWGKYAAEIRDSTRQGVRVWLGTFDSAEAA
Sbjct: 61  VKEEIEDGDIIVQDAQTQKEERAYRGVRRRPWGKYAAEIRDSTRQGVRVWLGTFDSAEAA 120

Query: 121 ALAYDQAAFCMRGALATLNFPVEVVRESLYEMKYRWEVGCSPVLALKRRHTIRRKKMAEA 180
           ALAYDQAAFCMRGALATLNFPVEVVRESLYEMKYRWEVGCSPVLALKRRHTIRRKKMAEA
Sbjct: 121 ALAYDQAAFCMRGALATLNFPVEVVRESLYEMKYRWEVGCSPVLALKRRHTIRRKKMAEA 180

Query: 181 EKMEKKMKKKKNVVVFEDLGVEYLEELLSLTS 213
           EKMEKKMKKKKNVVVFEDLGVEYLEELLSLTS
Sbjct: 181 EKMEKKMKKKKNVVVFEDLGVEYLEELLSLTS 210

[Close]

BLAST of Carg05117 vs. NCBI nr
Match: XP_023514563.1 (ethylene-responsive transcription factor 1B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 401.4 bits (1030), Expect = 5.0e-108
Identity = 206/212 (97.17%), Postives = 209/212 (98.58%), Query Frame = 0


Query: 1   MSLDNHLFGYQENFENFPGKQNSHLSFHFNYLEEMLFADMSLSSSSSSSDSSPCLSPEMH 60
           MSL NHLFGYQENFENFPGKQNSHLSFHFNYLEEMLFADMSLSSSSSSSDSSPCLSPEMH
Sbjct: 1   MSLHNHLFGYQENFENFPGKQNSHLSFHFNYLEEMLFADMSLSSSSSSSDSSPCLSPEMH 60

Query: 61  VKEEIEDGDIIVQDAQTQKEERAYRGVRRRPWGKYAAEIRDSTRQGVRVWLGTFDSAEAA 120
           VKEEIEDGDI+VQ+ +T KEERAYRGVRRRPWGKYAAEIRDSTRQGVRVWLGTFDSAEAA
Sbjct: 61  VKEEIEDGDIVVQEDRTPKEERAYRGVRRRPWGKYAAEIRDSTRQGVRVWLGTFDSAEAA 120

Query: 121 ALAYDQAAFCMRGALATLNFPVEVVRESLYEMKYRWEVGCSPVLALKRRHTIRRKKMAEA 180
           ALAYDQAAFCMRGALATLNFPVEVVRESLYEMKYRWEVGCSPVLALKRRHTIRRKKMAEA
Sbjct: 121 ALAYDQAAFCMRGALATLNFPVEVVRESLYEMKYRWEVGCSPVLALKRRHTIRRKKMAEA 180

Query: 181 EKMEKKMKKKKNVVVFEDLGVEYLEELLSLTS 213
           EKMEKKMKKKKNVVVFEDLGVEYLEELLSLTS
Sbjct: 181 EKMEKKMKKKKNVVVFEDLGVEYLEELLSLTS 212

[Close]

BLAST of Carg05117 vs. NCBI nr
Match: XP_023004364.1 (ethylene-responsive transcription factor 1B-like [Cucurbita maxima])

HSP 1 Score: 382.9 bits (982), Expect = 1.8e-102
Identity = 199/212 (93.87%), Postives = 203/212 (95.75%), Query Frame = 0


Query: 1   MSLDNHLFGYQENFENFPGKQNSHLSFHFNYLEEMLFADMSLSSSSSSSDSSPCLSPEMH 60
           MSLDNHLFGYQENF  FPGK+NSHLSF FNYLEEMLFADMS  SSSSSSDSSPCLSPEMH
Sbjct: 1   MSLDNHLFGYQENFRTFPGKENSHLSFDFNYLEEMLFADMSSLSSSSSSDSSPCLSPEMH 60

Query: 61  VKEEIEDGDIIVQDAQTQKEERAYRGVRRRPWGKYAAEIRDSTRQGVRVWLGTFDSAEAA 120
           VKEEIEDGDI+VQD +T KEERAYRGVRRRPWGKYAAEIRDSTRQGVRVWLGTFDSAEAA
Sbjct: 61  VKEEIEDGDIVVQDDRTPKEERAYRGVRRRPWGKYAAEIRDSTRQGVRVWLGTFDSAEAA 120

Query: 121 ALAYDQAAFCMRGALATLNFPVEVVRESLYEMKYRWEVGCSPVLALKRRHTIRRKKMAEA 180
           ALAYDQAAFCMRGALATLNFP EVVRESLYEMKYRWEVGCSPVLALKRRHTIRRKK+AEA
Sbjct: 121 ALAYDQAAFCMRGALATLNFPAEVVRESLYEMKYRWEVGCSPVLALKRRHTIRRKKIAEA 180

Query: 181 EKMEKKMKKKKNVVVFEDLGVEYLEELLSLTS 213
           EKMEKKM KKKNVVVFEDLGVEYLEELLSLTS
Sbjct: 181 EKMEKKM-KKKNVVVFEDLGVEYLEELLSLTS 211

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BLAST of Carg05117 vs. ExPASy Swiss-Prot
Match: A0A3Q7I5Y9 (Ethylene-response factor C3 OS=Solanum lycopersicum OX=4081 GN=ERF.C.3 PE=2 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 4.4e-43
Identity = 100/184 (54.35%), Postives = 133/184 (72.28%), Query Frame = 0


Query: 25  LSFHFNYLEEMLFADMSLSSSSSSSDSSPCLSPEMHVKEEIEDGDIIVQDAQTQKEERAY 84
           L F+ N  ++ML  ++    SS  + +S   S    +KEE       V   + +++++ Y
Sbjct: 16  LPFNVNDTQDMLLYNLVAEGSSQETVNS---SSSYGIKEE------EVTSYEEERKDKNY 75

Query: 85  RGVRRRPWGKYAAEIRDSTRQGVRVWLGTFDSAEAAALAYDQAAFCMRGALATLNFPVEV 144
           RGVR+RPWGKYAAEIRDSTR GVRVWLGTFD+AE AALAYDQAAF MRG++A LNFPVE+
Sbjct: 76  RGVRKRPWGKYAAEIRDSTRNGVRVWLGTFDNAEEAALAYDQAAFAMRGSMAILNFPVEI 135

Query: 145 VRESLYEMKYRWEVGCSPVLALKRRHTIRRKKMAEAEKMEKKMKKKKNVVVFEDLGVEYL 204
           V+ESL EMK R++  CSPV+ LK+R+++RRK ++      +K + +K+VVVFEDLG EYL
Sbjct: 136 VKESLNEMKCRFDGNCSPVIELKKRYSMRRKSVS------RKNRARKDVVVFEDLGAEYL 184

Query: 205 EELL 209
           EELL
Sbjct: 196 EELL 184

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BLAST of Carg05117 vs. ExPASy Swiss-Prot
Match: Q8LDC8 (Ethylene-responsive transcription factor 1B OS=Arabidopsis thaliana OX=3702 GN=ERF1B PE=1 SV=2)

HSP 1 Score: 169.9 bits (429), Expect = 3.2e-41
Identity = 102/185 (55.14%), Postives = 128/185 (69.19%), Query Frame = 0


Query: 41  SLSSSSSSSDSSPCL---SPEMHVKEEIEDG-----------DIIVQDAQTQKEERAYRG 100
           S SSSSS++ S P     S EM +   IE             D+ ++   ++K E++YRG
Sbjct: 25  SFSSSSSNNYSLPFNENDSEEMFLYGLIEQSTQQTYIDSDSQDLPIKSVSSRKSEKSYRG 84

Query: 101 VRRRPWGKYAAEIRDSTRQGVRVWLGTFDSAEAAALAYDQAAFCMRGALATLNFPVEVVR 160
           VRRRPWGK+AAEIRDSTR G+RVWLGTF+SAE AALAYDQAAF MRG+ A LNF  E V+
Sbjct: 85  VRRRPWGKFAAEIRDSTRNGIRVWLGTFESAEEAALAYDQAAFSMRGSSAILNFSAERVQ 144

Query: 161 ESLYEMKYRWEVGCSPVLALKRRHTIRRK---KMAEAEKMEKKMKKKKNVVVFEDLGVEY 209
           ESL E+KY +E GCSPV+ALKR+H++RR+   K  +    + +  K  NVVVFEDLG +Y
Sbjct: 145 ESLSEIKYTYEDGCSPVVALKRKHSMRRRMTNKKTKDSDFDHRSVKLDNVVVFEDLGEQY 204

[Close]

BLAST of Carg05117 vs. ExPASy Swiss-Prot
Match: Q8VYM0 (Ethylene-responsive transcription factor 15 OS=Arabidopsis thaliana OX=3702 GN=ERF15 PE=2 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 1.5e-38
Identity = 94/185 (50.81%), Postives = 120/185 (64.86%), Query Frame = 0


Query: 56  SPEMHVKEEIEDGDIIVQDAQTQ---KEERAYRGVRRRPWGKYAAEIRDSTRQGVRVWLG 115
           SPEM +KEEI++GD+   + + +    EER+YRGVR+RPWGK+AAEIRDSTR G+RVWLG
Sbjct: 55  SPEMMIKEEIQNGDVSNSEEEEKVGIDEERSYRGVRKRPWGKFAAEIRDSTRNGIRVWLG 114

Query: 116 TFDSAEAAALAYDQAAFCMRGALATLNFPVEVVRESLYEMK-YRWEVGCSPVLALKRRHT 175
           TFD AE AALAYDQAAF  +G+LATLNFPVEVVRESL +M+      G SPV+ALKR+H+
Sbjct: 115 TFDKAEEAALAYDQAAFATKGSLATLNFPVEVVRESLKKMENVNLHDGGSPVMALKRKHS 174

Query: 176 IRRKKMAE----------------------------AEKMEKKMKKKKNVVVFEDLGVEY 209
           +R +   +                            + K     ++   +VVFEDLG EY
Sbjct: 175 LRNRPRGKKRSSSSSSSSSNSSSCSSSSSTSSTSRSSSKQSVVKQESGTLVVFEDLGAEY 234

[Close]

BLAST of Carg05117 vs. ExPASy Swiss-Prot
Match: Q9LND1 (Ethylene-responsive transcription factor ERF094 OS=Arabidopsis thaliana OX=3702 GN=ERF094 PE=2 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 2.7e-32
Identity = 86/182 (47.25%), Postives = 116/182 (63.74%), Query Frame = 0


Query: 61  VKEEIEDGDIIVQDAQTQKEERAYRGVRRRPWGKYAAEIRDSTRQGVRVWLGTFDSAEAA 120
           +KEE ++  +  ++     EE++YRGVR+RPWGK+AAEIRDSTR+G+RVWLGTFD+AEAA
Sbjct: 64  IKEEGKEATVAAEE-----EEKSYRGVRKRPWGKFAAEIRDSTRKGIRVWLGTFDTAEAA 123

Query: 121 ALAYDQAAFCMRGALATLNFPVEVVRESLYEMK-YRWEVGCSPVLALKRRHTIR-----R 180
           ALAYDQAAF ++G+LA LNFP +VV ESL +M+      G SPV+ALKR+H++R     +
Sbjct: 124 ALAYDQAAFALKGSLAVLNFPADVVEESLRKMENVNLNDGESPVIALKRKHSMRNRPRGK 183

Query: 181 KK--------------------------MAEAEKMEKKMKKKKN--VVVFEDLGVEYLEE 209
           KK                           + + K    M ++ N  +VV EDLG EYLEE
Sbjct: 184 KKSSSSSTLTSSPSSSSSYSSSSSSSSLSSRSRKQSVVMTQESNTTLVVLEDLGAEYLEE 240

[Close]

BLAST of Carg05117 vs. ExPASy Swiss-Prot
Match: O04681 (Pathogenesis-related genes transcriptional activator PTI5 OS=Solanum lycopersicum OX=4081 GN=PTI5 PE=2 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 2.5e-22
Identity = 51/68 (75.00%), Postives = 57/68 (83.82%), Query Frame = 0


Query: 82  RAYRGVRRRPWGKYAAEIRDSTRQGVRVWLGTFDSAEAAALAYDQAAFCMRGALATLNFP 141
           + YRGVRRRPWGKYAAEIRDS R G RVWLGTF++AE AALAYD+AAF MRGA A LNFP
Sbjct: 57  KKYRGVRRRPWGKYAAEIRDSARHGARVWLGTFETAEEAALAYDRAAFRMRGAKALLNFP 116

Query: 142 VEVVRESL 150
            E+V  S+
Sbjct: 117 SEIVNASV 124

[Close]

BLAST of Carg05117 vs. ExPASy TrEMBL
Match: A0A6J1H6Z0 (ethylene-responsive transcription factor 1B-like OS=Cucurbita moschata OX=3662 GN=LOC111460680 PE=4 SV=1)

HSP 1 Score: 401.7 bits (1031), Expect = 1.8e-108
Identity = 209/212 (98.58%), Postives = 209/212 (98.58%), Query Frame = 0


Query: 1   MSLDNHLFGYQENFENFPGKQNSHLSFHFNYLEEMLFADMSLSSSSSSSDSSPCLSPEMH 60
           MSL NHLFGYQENFENFPGKQNSHLSFHFNYLEEMLFADMSL  SSSSSDSSPCLSPEMH
Sbjct: 1   MSLHNHLFGYQENFENFPGKQNSHLSFHFNYLEEMLFADMSL--SSSSSDSSPCLSPEMH 60

Query: 61  VKEEIEDGDIIVQDAQTQKEERAYRGVRRRPWGKYAAEIRDSTRQGVRVWLGTFDSAEAA 120
           VKEEIEDGDIIVQDAQTQKEERAYRGVRRRPWGKYAAEIRDSTRQGVRVWLGTFDSAEAA
Sbjct: 61  VKEEIEDGDIIVQDAQTQKEERAYRGVRRRPWGKYAAEIRDSTRQGVRVWLGTFDSAEAA 120

Query: 121 ALAYDQAAFCMRGALATLNFPVEVVRESLYEMKYRWEVGCSPVLALKRRHTIRRKKMAEA 180
           ALAYDQAAFCMRGALATLNFPVEVVRESLYEMKYRWEVGCSPVLALKRRHTIRRKKMAEA
Sbjct: 121 ALAYDQAAFCMRGALATLNFPVEVVRESLYEMKYRWEVGCSPVLALKRRHTIRRKKMAEA 180

Query: 181 EKMEKKMKKKKNVVVFEDLGVEYLEELLSLTS 213
           EKMEKKMKKKKNVVVFEDLGVEYLEELLSLTS
Sbjct: 181 EKMEKKMKKKKNVVVFEDLGVEYLEELLSLTS 210

[Close]

BLAST of Carg05117 vs. ExPASy TrEMBL
Match: A0A6J1KW25 (ethylene-responsive transcription factor 1B-like OS=Cucurbita maxima OX=3661 GN=LOC111497706 PE=4 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 8.8e-103
Identity = 199/212 (93.87%), Postives = 203/212 (95.75%), Query Frame = 0


Query: 1   MSLDNHLFGYQENFENFPGKQNSHLSFHFNYLEEMLFADMSLSSSSSSSDSSPCLSPEMH 60
           MSLDNHLFGYQENF  FPGK+NSHLSF FNYLEEMLFADMS  SSSSSSDSSPCLSPEMH
Sbjct: 1   MSLDNHLFGYQENFRTFPGKENSHLSFDFNYLEEMLFADMSSLSSSSSSDSSPCLSPEMH 60

Query: 61  VKEEIEDGDIIVQDAQTQKEERAYRGVRRRPWGKYAAEIRDSTRQGVRVWLGTFDSAEAA 120
           VKEEIEDGDI+VQD +T KEERAYRGVRRRPWGKYAAEIRDSTRQGVRVWLGTFDSAEAA
Sbjct: 61  VKEEIEDGDIVVQDDRTPKEERAYRGVRRRPWGKYAAEIRDSTRQGVRVWLGTFDSAEAA 120

Query: 121 ALAYDQAAFCMRGALATLNFPVEVVRESLYEMKYRWEVGCSPVLALKRRHTIRRKKMAEA 180
           ALAYDQAAFCMRGALATLNFP EVVRESLYEMKYRWEVGCSPVLALKRRHTIRRKK+AEA
Sbjct: 121 ALAYDQAAFCMRGALATLNFPAEVVRESLYEMKYRWEVGCSPVLALKRRHTIRRKKIAEA 180

Query: 181 EKMEKKMKKKKNVVVFEDLGVEYLEELLSLTS 213
           EKMEKKM KKKNVVVFEDLGVEYLEELLSLTS
Sbjct: 181 EKMEKKM-KKKNVVVFEDLGVEYLEELLSLTS 211

[Close]

BLAST of Carg05117 vs. ExPASy TrEMBL
Match: A0A0A0K4U4 (AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G375820 PE=4 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 1.8e-71
Identity = 163/231 (70.56%), Postives = 182/231 (78.79%), Query Frame = 0


Query: 1   MSLDNHLFG-YQENF-----ENFPGKQNSHLSFHFNYLEEMLFADMSLSSSS-SSSDSSP 60
           MS+ +  FG YQENF      N         SF FNYL +MLF DM  +  + SSSDSS 
Sbjct: 1   MSILSDFFGNYQENFLWNNDHNQSFISGEETSFDFNYLNDMLFDDMVFAEETMSSSDSSS 60

Query: 61  CLSP-EMHVKE-----EIEDG----DIIVQDAQTQKEERAYRGVRRRPWGKYAAEIRDST 120
           C SP E+ VKE     E+E+G    +++V++ Q+ KEER YRGVRRRPWGKYAAEIRDST
Sbjct: 61  CSSPIELQVKEEVVEVEVEEGSVCDEVVVEEDQSHKEERVYRGVRRRPWGKYAAEIRDST 120

Query: 121 RQGVRVWLGTFDSAEAAALAYDQAAFCMRGALATLNFPVEVVRESLYEMKYRWEVGCSPV 180
           RQGVRVWLGTFDSAEAAALAYDQAAFCMRG LATLNFP+EVVRESL EMKYRWEVG SPV
Sbjct: 121 RQGVRVWLGTFDSAEAAALAYDQAAFCMRGGLATLNFPIEVVRESLREMKYRWEVGSSPV 180

Query: 181 LALKRRHTIRRKKMAEAEKMEKKM--KKKKNVVVFEDLGVEYLEELLSLTS 213
           LALKRRH+I+RK+MAE EKMEKKM  KKKK+VVVFEDLGVEYLEELLSLTS
Sbjct: 181 LALKRRHSIQRKRMAEVEKMEKKMMKKKKKSVVVFEDLGVEYLEELLSLTS 231

[Close]

BLAST of Carg05117 vs. ExPASy TrEMBL
Match: A0A5D3C9K8 (Ethylene-responsive transcription factor 1B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold886G00670 PE=4 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 1.9e-68
Identity = 163/237 (68.78%), Postives = 183/237 (77.22%), Query Frame = 0


Query: 1   MSLDNHLFG-YQENF--------ENFPGKQNSHLSFHFNYLEEMLFADM---SLSSSSSS 60
           MS+ + LFG YQENF            G++ S  SF FNYL +MLF DM   +   + SS
Sbjct: 1   MSIVSDLFGNYQENFLWNNDHCGSLISGQEES--SFDFNYLNKMLFDDMVSVAAEETMSS 60

Query: 61  SDSSPCLSP-EMHVKE-----EIEDG----DIIVQDAQTQKEERAYRGVRRRPWGKYAAE 120
           SDSS C S   M VKE     E+++G    D++V++ Q+ KEER YRGVRRRPWGKYAAE
Sbjct: 61  SDSSSCSSSIVMQVKEEVVEVEVDEGSFSEDVVVEEDQSHKEERVYRGVRRRPWGKYAAE 120

Query: 121 IRDSTRQGVRVWLGTFDSAEAAALAYDQAAFCMRGALATLNFPVEVVRESLYEMKYRWEV 180
           IRDSTRQGVRVWLGTFDSAEAAALAYDQAAFCMRG LATLNFP+EVVRESL EMKYR EV
Sbjct: 121 IRDSTRQGVRVWLGTFDSAEAAALAYDQAAFCMRGGLATLNFPIEVVRESLREMKYRREV 180

Query: 181 GCSPVLALKRRHTIRRKKMAEAEKMEKKM---KKKKNVVVFEDLGVEYLEELLSLTS 213
           G SPVLALKRRH+I+RK+MAE EKMEKKM   KKKK+VVVFEDLGVEYLEELLSLTS
Sbjct: 181 GSSPVLALKRRHSIQRKRMAEVEKMEKKMMKKKKKKSVVVFEDLGVEYLEELLSLTS 235

[Close]

BLAST of Carg05117 vs. ExPASy TrEMBL
Match: A0A1S3BQG1 (ethylene-responsive transcription factor 1B-like OS=Cucumis melo OX=3656 GN=LOC103492600 PE=4 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 1.9e-68
Identity = 163/237 (68.78%), Postives = 183/237 (77.22%), Query Frame = 0


Query: 1   MSLDNHLFG-YQENF--------ENFPGKQNSHLSFHFNYLEEMLFADM---SLSSSSSS 60
           MS+ + LFG YQENF            G++ S  SF FNYL +MLF DM   +   + SS
Sbjct: 1   MSIVSDLFGNYQENFLWNNDHCGSLISGQEES--SFDFNYLNKMLFDDMVSVAAEETMSS 60

Query: 61  SDSSPCLSP-EMHVKE-----EIEDG----DIIVQDAQTQKEERAYRGVRRRPWGKYAAE 120
           SDSS C S   M VKE     E+++G    D++V++ Q+ KEER YRGVRRRPWGKYAAE
Sbjct: 61  SDSSSCSSSIVMQVKEEVVEVEVDEGSFSEDVVVEEDQSHKEERVYRGVRRRPWGKYAAE 120

Query: 121 IRDSTRQGVRVWLGTFDSAEAAALAYDQAAFCMRGALATLNFPVEVVRESLYEMKYRWEV 180
           IRDSTRQGVRVWLGTFDSAEAAALAYDQAAFCMRG LATLNFP+EVVRESL EMKYR EV
Sbjct: 121 IRDSTRQGVRVWLGTFDSAEAAALAYDQAAFCMRGGLATLNFPIEVVRESLREMKYRREV 180

Query: 181 GCSPVLALKRRHTIRRKKMAEAEKMEKKM---KKKKNVVVFEDLGVEYLEELLSLTS 213
           G SPVLALKRRH+I+RK+MAE EKMEKKM   KKKK+VVVFEDLGVEYLEELLSLTS
Sbjct: 181 GSSPVLALKRRHSIQRKRMAEVEKMEKKMMKKKKKKSVVVFEDLGVEYLEELLSLTS 235

[Close]

BLAST of Carg05117 vs. TAIR 10
Match: AT3G23240.1 (ethylene response factor 1 )

HSP 1 Score: 169.9 bits (429), Expect = 2.3e-42
Identity = 102/185 (55.14%), Postives = 128/185 (69.19%), Query Frame = 0


Query: 41  SLSSSSSSSDSSPCL---SPEMHVKEEIEDG-----------DIIVQDAQTQKEERAYRG 100
           S SSSSS++ S P     S EM +   IE             D+ ++   ++K E++YRG
Sbjct: 25  SFSSSSSNNYSLPFNENDSEEMFLYGLIEQSTQQTYIDSDSQDLPIKSVSSRKSEKSYRG 84

Query: 101 VRRRPWGKYAAEIRDSTRQGVRVWLGTFDSAEAAALAYDQAAFCMRGALATLNFPVEVVR 160
           VRRRPWGK+AAEIRDSTR G+RVWLGTF+SAE AALAYDQAAF MRG+ A LNF  E V+
Sbjct: 85  VRRRPWGKFAAEIRDSTRNGIRVWLGTFESAEEAALAYDQAAFSMRGSSAILNFSAERVQ 144

Query: 161 ESLYEMKYRWEVGCSPVLALKRRHTIRRK---KMAEAEKMEKKMKKKKNVVVFEDLGVEY 209
           ESL E+KY +E GCSPV+ALKR+H++RR+   K  +    + +  K  NVVVFEDLG +Y
Sbjct: 145 ESLSEIKYTYEDGCSPVVALKRKHSMRRRMTNKKTKDSDFDHRSVKLDNVVVFEDLGEQY 204

[Close]

BLAST of Carg05117 vs. TAIR 10
Match: AT2G31230.1 (ethylene-responsive element binding factor 15 )

HSP 1 Score: 161.0 bits (406), Expect = 1.1e-39
Identity = 94/185 (50.81%), Postives = 120/185 (64.86%), Query Frame = 0


Query: 56  SPEMHVKEEIEDGDIIVQDAQTQ---KEERAYRGVRRRPWGKYAAEIRDSTRQGVRVWLG 115
           SPEM +KEEI++GD+   + + +    EER+YRGVR+RPWGK+AAEIRDSTR G+RVWLG
Sbjct: 55  SPEMMIKEEIQNGDVSNSEEEEKVGIDEERSYRGVRKRPWGKFAAEIRDSTRNGIRVWLG 114

Query: 116 TFDSAEAAALAYDQAAFCMRGALATLNFPVEVVRESLYEMK-YRWEVGCSPVLALKRRHT 175
           TFD AE AALAYDQAAF  +G+LATLNFPVEVVRESL +M+      G SPV+ALKR+H+
Sbjct: 115 TFDKAEEAALAYDQAAFATKGSLATLNFPVEVVRESLKKMENVNLHDGGSPVMALKRKHS 174

Query: 176 IRRKKMAE----------------------------AEKMEKKMKKKKNVVVFEDLGVEY 209
           +R +   +                            + K     ++   +VVFEDLG EY
Sbjct: 175 LRNRPRGKKRSSSSSSSSSNSSSCSSSSSTSSTSRSSSKQSVVKQESGTLVVFEDLGAEY 234

[Close]

BLAST of Carg05117 vs. TAIR 10
Match: AT1G06160.1 (octadecanoid-responsive Arabidopsis AP2/ERF 59 )

HSP 1 Score: 140.2 bits (352), Expect = 1.9e-33
Identity = 86/182 (47.25%), Postives = 116/182 (63.74%), Query Frame = 0


Query: 61  VKEEIEDGDIIVQDAQTQKEERAYRGVRRRPWGKYAAEIRDSTRQGVRVWLGTFDSAEAA 120
           +KEE ++  +  ++     EE++YRGVR+RPWGK+AAEIRDSTR+G+RVWLGTFD+AEAA
Sbjct: 64  IKEEGKEATVAAEE-----EEKSYRGVRKRPWGKFAAEIRDSTRKGIRVWLGTFDTAEAA 123

Query: 121 ALAYDQAAFCMRGALATLNFPVEVVRESLYEMK-YRWEVGCSPVLALKRRHTIR-----R 180
           ALAYDQAAF ++G+LA LNFP +VV ESL +M+      G SPV+ALKR+H++R     +
Sbjct: 124 ALAYDQAAFALKGSLAVLNFPADVVEESLRKMENVNLNDGESPVIALKRKHSMRNRPRGK 183

Query: 181 KK--------------------------MAEAEKMEKKMKKKKN--VVVFEDLGVEYLEE 209
           KK                           + + K    M ++ N  +VV EDLG EYLEE
Sbjct: 184 KKSSSSSTLTSSPSSSSSYSSSSSSSSLSSRSRKQSVVMTQESNTTLVVLEDLGAEYLEE 240

[Close]

BLAST of Carg05117 vs. TAIR 10
Match: AT5G43410.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 98.6 bits (244), Expect = 6.4e-21
Identity = 48/64 (75.00%), Postives = 51/64 (79.69%), Query Frame = 0


Query: 80  EERAYRGVRRRPWGKYAAEIRDSTRQGVRVWLGTFDSAEAAALAYDQAAFCMRGALATLN 139
           E   YRGVRRRPWGKYAAEIRDS + G RVWLGTFD+AE AA AYDQAA+ MRG  A LN
Sbjct: 11  EHGKYRGVRRRPWGKYAAEIRDSRKHGERVWLGTFDTAEEAARAYDQAAYSMRGQAAILN 70

Query: 140 FPVE 144
           FP E
Sbjct: 71  FPHE 74

[Close]

BLAST of Carg05117 vs. TAIR 10
Match: AT4G17500.1 (ethylene responsive element binding factor 1 )

HSP 1 Score: 98.2 bits (243), Expect = 8.4e-21
Identity = 63/145 (43.45%), Postives = 83/145 (57.24%), Query Frame = 0


Query: 10  YQENFENFPGKQN-SHLSFHFNYLEEMLFADMSLSSSSSSSDSSPCLSPEMHVKEEIEDG 69
           + E++ + P K+N S     +  L +        SSSSS  D S   S ++   E     
Sbjct: 64  FTESWGDLPLKENDSEDMLVYGILNDAFHGGWEPSSSSSDEDRSSFPSVKIETPESFAAV 123

Query: 70  DII---------VQDAQTQKEERAYRGVRRRPWGKYAAEIRDSTRQGVRVWLGTFDSAEA 129
           D +         V  A T  + + YRGVR+RPWGK+AAEIRD  + G RVWLGTF++AE 
Sbjct: 124 DSVPVKKEKTSPVSAAVTAAKGKHYRGVRQRPWGKFAAEIRDPAKNGARVWLGTFETAED 183

Query: 130 AALAYDQAAFCMRGALATLNFPVEV 145
           AALAYD+AAF MRG+ A LNFP+ V
Sbjct: 184 AALAYDRAAFRMRGSRALLNFPLRV 208

The following BLAST results are available for this feature:

BLAST of Carg05117 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7025485.1	2.8e-111	100.00	Ethylene-responsive transcription factor 1B, partial [Cucurbita argyrosperma sub...	[more]
KAG6593075.1	1.8e-110	99.53	Ethylene-response factor C3, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022959670.1	3.8e-108	98.58	ethylene-responsive transcription factor 1B-like [Cucurbita moschata]	[more]
XP_023514563.1	5.0e-108	97.17	ethylene-responsive transcription factor 1B-like [Cucurbita pepo subsp. pepo]	[more]
XP_023004364.1	1.8e-102	93.87	ethylene-responsive transcription factor 1B-like [Cucurbita maxima]	[more]

BLAST of Carg05117 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A3Q7I5Y9	4.4e-43	54.35	Ethylene-response factor C3 OS=Solanum lycopersicum OX=4081 GN=ERF.C.3 PE=2 SV=1	[more]
Q8LDC8	3.2e-41	55.14	Ethylene-responsive transcription factor 1B OS=Arabidopsis thaliana OX=3702 GN=E...	[more]
Q8VYM0	1.5e-38	50.81	Ethylene-responsive transcription factor 15 OS=Arabidopsis thaliana OX=3702 GN=E...	[more]
Q9LND1	2.7e-32	47.25	Ethylene-responsive transcription factor ERF094 OS=Arabidopsis thaliana OX=3702 ...	[more]
O04681	2.5e-22	75.00	Pathogenesis-related genes transcriptional activator PTI5 OS=Solanum lycopersicu...	[more]

BLAST of Carg05117 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H6Z0	1.8e-108	98.58	ethylene-responsive transcription factor 1B-like OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1KW25	8.8e-103	93.87	ethylene-responsive transcription factor 1B-like OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A0A0K4U4	1.8e-71	70.56	AP2/ERF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G375820 PE=...	[more]
A0A5D3C9K8	1.9e-68	68.78	Ethylene-responsive transcription factor 1B-like OS=Cucumis melo var. makuwa OX=...	[more]
A0A1S3BQG1	1.9e-68	68.78	ethylene-responsive transcription factor 1B-like OS=Cucumis melo OX=3656 GN=LOC1...	[more]

BLAST of Carg05117 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G23240.1	2.3e-42	55.14	ethylene response factor 1	[more]
AT2G31230.1	1.1e-39	50.81	ethylene-responsive element binding factor 15	[more]
AT1G06160.1	1.9e-33	47.25	octadecanoid-responsive Arabidopsis AP2/ERF 59	[more]
AT5G43410.1	6.4e-21	75.00	Integrase-type DNA-binding superfamily protein	[more]
AT4G17500.1	8.4e-21	43.45	ethylene responsive element binding factor 1	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 173..193
None	No IPR available	PANTHER	PTHR31190:SF132	ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR 1B	coord: 15..210
IPR001471	AP2/ERF domain	PRINTS	PR00367	ETHRSPELEMNT	coord: 84..95 score: 71.67 coord: 107..123 score: 65.88
IPR001471	AP2/ERF domain	SMART	SM00380	rav1_2	coord: 83..147 e-value: 1.2E-36 score: 137.7
IPR001471	AP2/ERF domain	PFAM	PF00847	AP2	coord: 84..133 e-value: 9.4E-15 score: 54.6
IPR001471	AP2/ERF domain	PROSITE	PS51032	AP2_ERF	coord: 83..141 score: 22.83984
IPR001471	AP2/ERF domain	CDD	cd00018	AP2	coord: 84..142 e-value: 8.06072E-21 score: 80.0083
IPR036955	AP2/ERF domain superfamily	GENE3D	3.30.730.10	AP2/ERF domain	coord: 83..144 e-value: 1.7E-26 score: 94.1
IPR044808	Ethylene-responsive transcription factor	PANTHER	PTHR31190	DNA-BINDING DOMAIN	coord: 15..210
IPR016177	DNA-binding domain superfamily	SUPERFAMILY	54171	DNA-binding domain	coord: 83..143

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg05117-RA	Carg05117-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009873	ethylene-activated signaling pathway
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003700	DNA-binding transcription factor activity