Carg05071 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg05071
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionAsparagine--tRNA ligase
LocationCarg_Chr08: 1406891 .. 1410684 (+)
RNA-Seq ExpressionCarg05071
SyntenyCarg05071
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAACGATCTGCAAAGCTATGAAATGAACAAATCTCCAAGAAACTCTCGAACTTGGAAGGATTCCAATTCAAACTCTGTAATCTCTCTCAGTTTATCCCACGAAACAGCCGAAATCATTCCTCTGAAACAATCCCTTTCCCTCCATTAACATCCTCTCTCGATTTCCCCATCTTCTTCCCCAAGAAAAGCATCTCGATTTTGTTCTATATCTCTTCATCCATGGCTCATCTTCTTCACCCATCAATTCACCGTTTCTGTTATCTTTCTTCCTCCCACACTCTTCAATGGCCGCCGAACACCCTACTGTTTTCACCCCTGTCACCTTCTCCAAGTACTCGAAGCGGGTTCTTCTCAAAACTGTGCTGGACTGCAGCGATGGTGGTTTGGGGTTTGTGGATCAGAAATTGGTGATCGGAGGCTGGGTGAAGTCCTCCAAGGAGGTTTTTAGAGAGGCGGCGGTGCCGGGTGATGCTCTGCCGCCTAGGGCTAAGGATGTTAGCTGCATTGAGATTTTACAGTCGCGGATTCCGATCGTTAGGTCTTTGCTTAGGATGCTGTTGGAGACGTCTGTTCATAAGCCGCCGCCGCCGCTTCCTTCCACTACTTTTCTTGTTGTTAACGATGGCTCTTGTGTTGCAAGCCTTCAGGTAACCTCTAATCTATGCACGCCAACTGTTTGCAAGTTTGTTCTCAAAGTATAGAAACTTGCAGGTTGCTGTTGAGTCCTCGTTACATCATCCTGGCCAGCTGATGCCTACGGGAACTTGCATCTTGGTGGAAGGCATATTGAAGCGGGCACCGGCACCTGGGAAACATTTGGTTCGGCTTGAAGTGGAGAAAATCCTTCACGTAGGGAAGGTTGAACCGGAAAAGTACCCACTAACCCAAAAGAAGTTGCTAATAGATCTTTTGAGAAAATTTTCTTATTTTCGTCCTCGAACAACAACGGTAATTCGTACAAGCTTCAACCATACATTGCATTTCTGTTCTGTTTTATGGGTCTGTTTGCATGCCTATTATTATACCGCCATTAGTTTTAAGAATTTGCTTAAGCAATAGCAGCATGTGAATTAGAGTAAGAGCCTTGAGATCAGAAAAATGGTGATACAGTTCAATCTCTGGTAAAGGAAAGGATTTATATAGTAGGGTTGTCCATGAAATGAAATTAGTCGAGGTGTACGCAAGCTGAACTGAACATCGCAGATATTAAAAGGGAAAACCAAGAAGATTTATATGTATAGGTTGATGTTCTTGATCACGGCATTTAATGAACTGAATAGTATCGATGCAAAGGGCTCTAGGGCTGAAGTTTGAGAAGAGAGTTTTTCATATTTTAACCATTGAAGCCAATGAAGTATTGGATCTTGATCTTCATTGATATTCATTACCTTATGCAGGTAGCAACTGTTATGAGAGTTCATAATGCCTTGACTTTTGCAACTCACACATTCTTCCAAAATCATGGCTTTCTTTATGTGCAATTACCGACCATCACGACCACAGACAGCGAGGGATTCAGTGAAAAGTTCCACGTTACAACCCGTGCCGGCAATTCAGAAGTTCAGAAAATAGAATCAACAGAAGACAAGGAAACTGATGGTGTGAGTCTTGAAACTGTTAAGGCTGCTGCAATAGAGAAAAAAAATATTGTCAATGAACTCGAGAGAAGTGAAAGCAACAGGGAAGCCTTAGCTGCTGCAGTTCAGGATTTAAAGAAGACAAATGAACTTGCATTACAATTAGAAGCCAGAGGAAAACAAAAATCCAAATTGAAGAAGATAAAGTTCACGTTTCTGAAAATTTCTTCCCACAAGAAACTTATCTCACGGTGTCTGGACAACTCCATTTGGAGACCTATGCGTGTGCCCTTGGAAATGTTTACTCATGCGGACCGAGGTTTCGGGCAGATAGAATCGAGTCGGCAAAACATGCTGCTGAAATGTGGATGGTGGAGGTCGAAATTGCTTTTGCTGAACTCGAGGTATGTATCTGTGATCTTATACTTATTCCTTTAGATTGCTTAGTATTTGACATATAAATTATGGGTCTAAGAGTCCATGTTGATTTAGCCATTAAAACCTTTATTTTCTGTGATCAGTAGTTGTTTCTAATATCATTGCACCATTGAAATGAAGAATTTCCATCTTTTAACTTGCGTTTTGTATCATCGTTTGGGGAGTCCAGGATGTCATCAACTGTGCTGATGACCTTGTTAAGTTCCTCTCCAAATGGGTGTCGGAGCACTGTCTGGATGATATGAAATTCGTGCAAAAACGAATCGACAAAACGAGCATTCATTGCCTTGAATCAACCATCAAAAGTTCATTTGAGAAGGTCTCTTACACTGCTGCAATAAAAATTCTGAATAAGGTGAATCTTCGAACAGATGCCTCGGTTTTTCATTTCCACCCGTTTATCTGAATATTGAAAAACAAAATTCTTCACTGTCAGGCAACCGATCAAAAATCTCAATGCTCGAAAAGGCTTAAATTTGGTGCTGCTTTAACTGATGATCATCTCAGGTGTGAAAATGCTAGTACAACACAAGCAAATTGATATTTTTTCATTTATCAAAAGTTATTTATTTTGGTCACATGAAACACAGTTATCTTGCTGATGCCTTCTTTAAGAAGCCTGTGATAGTATATAACTACCCAAAAGAAACCAAGCCATTTAATGTGCGACTGAATGATGATGGGAAAACAGTGGCAGCATTTGATGTAGTTCTTCCAAAGGTTAGCACTTTACTTTTTGGATAGATTCCGATTCAATGAAAAATTATTTCTTAAACTCGGGCCCGACTGTTGTAGGGTGGAGTAGTAATTTCTGGCAGCCAAAAGGAGGAGCGTCTCGATGTATTGTTGACAAGGTATAAATTATTCATCCGAAACGTAGACACTTTCACATCTTTTATTTATTTACTAGATCCATAGACATCTACATAGACACGCGTTTATATCCACGTGTAGTAGGTTAGATGTCTAGATTTAGGTTAACCATTATAGGTCGGCTTATGGTCAATAAAGACCATGAGAATAATAAAAGGTTTAAAGAGAATAAGTTGATCATAGCGACTACCCACCAACCAAATGTAATAGAATCATGCAGTTACCTCGTGAGATTAGTCGAAGTCTGTGCAAGCTGACACATATATATCGTAATAACGAATAATAAATTTAGGCCATCGACATTATCCACGCATGTCTAAAGTAGTTTTTGATGCAAATTTTACCCGAAAAAAAAGAGAGAACATCTTAAAGTTGAAGAATCAAAGTAAAAAAAAAAAAAAGTCTAATTATGAGCTGTTCTAGTGTGTGTCACTAGGCATGTGAAAAAAGTAAATATGCTTTTAAGAAATGGGTTGAAACTCCATGATAACTGTCCTAGAAAGTTATTGGTTTGTTGTAATATGGATGTTGACACACAGGATGAAGGAGATGGGGCTGCCAAGAGAGCAGTACGAGTGGTACTTGGAGATTCGAAGGCATGGAACAGTGAAGCACTCTGGTTTTAGTTTGGCATTTGATCGTTTCGTTCTGTTTACTACTGGCCTTACTGATATCAAAGATGCTATTCCGTTTCCAAGGAGTCTTGGAAAGGTCAGCCATTAAACCGAGTTCAAGCCCAAATTTCTAGATCAATGACCTCATATGCCTGCTGCTGCTGAAATGGAACTCAAAAATGACTTGTGTTTGTTTCTCCCTAAGATTTGTTCTTGTAAATAACAGAGAATTTGGAATAAAATGAGATATCTTTATGGACTCGTTCATGAGTTTATACTTACCAACATTGAT

mRNA sequence

AAACGATCTGCAAAGCTATGAAATGAACAAATCTCCAAGAAACTCTCGAACTTGGAAGGATTCCAATTCAAACTCTGTAATCTCTCTCAGTTTATCCCACGAAACAGCCGAAATCATTCCTCTGAAACAATCCCTTTCCCTCCATTAACATCCTCTCTCGATTTCCCCATCTTCTTCCCCAAGAAAAGCATCTCGATTTTGTTCTATATCTCTTCATCCATGGCTCATCTTCTTCACCCATCAATTCACCGTTTCTGTTATCTTTCTTCCTCCCACACTCTTCAATGGCCGCCGAACACCCTACTGTTTTCACCCCTGTCACCTTCTCCAAGTACTCGAAGCGGGTTCTTCTCAAAACTGTGCTGGACTGCAGCGATGGTGGTTTGGGGTTTGTGGATCAGAAATTGGTGATCGGAGGCTGGGTGAAGTCCTCCAAGGAGGTTTTTAGAGAGGCGGCGGTGCCGGGTGATGCTCTGCCGCCTAGGGCTAAGGATGTTAGCTGCATTGAGATTTTACAGTCGCGGATTCCGATCGTTAGGTCTTTGCTTAGGATGCTGTTGGAGACGTCTGTTCATAAGCCGCCGCCGCCGCTTCCTTCCACTACTTTTCTTGTTGTTAACGATGGCTCTTGTGTTGCAAGCCTTCAGGTTGCTGTTGAGTCCTCGTTACATCATCCTGGCCAGCTGATGCCTACGGGAACTTGCATCTTGGTGGAAGGCATATTGAAGCGGGCACCGGCACCTGGGAAACATTTGGTTCGGCTTGAAGTGGAGAAAATCCTTCACGTAGGGAAGGTTGAACCGGAAAAGTACCCACTAACCCAAAAGAAGTTGCTAATAGATCTTTTGAGAAAATTTTCTTATTTTCGTCCTCGAACAACAACGGTAGCAACTGTTATGAGAGTTCATAATGCCTTGACTTTTGCAACTCACACATTCTTCCAAAATCATGGCTTTCTTTATGTGCAATTACCGACCATCACGACCACAGACAGCGAGGGATTCAGTGAAAAGTTCCACGTTACAACCCGTGCCGGCAATTCAGAAGTTCAGAAAATAGAATCAACAGAAGACAAGGAAACTGATGGTGTGAGTCTTGAAACTGTTAAGGCTGCTGCAATAGAGAAAAAAAATATTGTCAATGAACTCGAGAGAAGTGAAAGCAACAGGGAAGCCTTAGCTGCTGCAGTTCAGGATTTAAAGAAGACAAATGAACTTGCATTACAATTAGAAGCCAGAGGAAAACAAAAATCCAAATTGAAGAAGATAAAGTTCACGTTTCTGAAAATTTCTTCCCACAAGAAACTTATCTCACGGTGTCTGGACAACTCCATTTGGAGACCTATGCGTGTGCCCTTGGAAATGTTTACTCATGCGGACCGAGATAGAATCGAGTCGGCAAAACATGCTGCTGAAATGTGGATGGTGGAGGTCGAAATTGCTTTTGCTGAACTCGAGGATGTCATCAACTGTGCTGATGACCTTGTTAAGTTCCTCTCCAAATGGGTGTCGGAGCACTGTCTGGATGATATGAAATTCGTGCAAAAACGAATCGACAAAACGAGCATTCATTGCCTTGAATCAACCATCAAAAGTTCATTTGAGAAGGTCTCTTACACTGCTGCAATAAAAATTCTGAATAAGGTGAATCTTCGAACAGATGCCTCGGCAACCGATCAAAAATCTCAATGCTCGAAAAGGCTTAAATTTGGTGCTGCTTTAACTGATGATCATCTCAGTTATCTTGCTGATGCCTTCTTTAAGAAGCCTGTGATAGTATATAACTACCCAAAAGAAACCAAGCCATTTAATGTGCGACTGAATGATGATGGGAAAACAGTGGCAGCATTTGATGTAGTTCTTCCAAAGGGTGGAGTAGTAATTTCTGGCAGCCAAAAGGAGGAGCGTCTCGATGTATTGTTGACAAGGATGAAGGAGATGGGGCTGCCAAGAGAGCAGTACGAGTGGTACTTGGAGATTCGAAGGCATGGAACAGTGAAGCACTCTGGTTTTAGTTTGGCATTTGATCGTTTCGTTCTGTTTACTACTGGCCTTACTGATATCAAAGATGCTATTCCGTTTCCAAGGAGTCTTGGAAAGGTCAGCCATTAAACCGAGTTCAAGCCCAAATTTCTAGATCAATGACCTCATATGCCTGCTGCTGCTGAAATGGAACTCAAAAATGACTTGTGTTTGTTTCTCCCTAAGATTTGTTCTTGTAAATAACAGAGAATTTGGAATAAAATGAGATATCTTTATGGACTCGTTCATGAGTTTATACTTACCAACATTGAT

Coding sequence (CDS)

ATGGCCGCCGAACACCCTACTGTTTTCACCCCTGTCACCTTCTCCAAGTACTCGAAGCGGGTTCTTCTCAAAACTGTGCTGGACTGCAGCGATGGTGGTTTGGGGTTTGTGGATCAGAAATTGGTGATCGGAGGCTGGGTGAAGTCCTCCAAGGAGGTTTTTAGAGAGGCGGCGGTGCCGGGTGATGCTCTGCCGCCTAGGGCTAAGGATGTTAGCTGCATTGAGATTTTACAGTCGCGGATTCCGATCGTTAGGTCTTTGCTTAGGATGCTGTTGGAGACGTCTGTTCATAAGCCGCCGCCGCCGCTTCCTTCCACTACTTTTCTTGTTGTTAACGATGGCTCTTGTGTTGCAAGCCTTCAGGTTGCTGTTGAGTCCTCGTTACATCATCCTGGCCAGCTGATGCCTACGGGAACTTGCATCTTGGTGGAAGGCATATTGAAGCGGGCACCGGCACCTGGGAAACATTTGGTTCGGCTTGAAGTGGAGAAAATCCTTCACGTAGGGAAGGTTGAACCGGAAAAGTACCCACTAACCCAAAAGAAGTTGCTAATAGATCTTTTGAGAAAATTTTCTTATTTTCGTCCTCGAACAACAACGGTAGCAACTGTTATGAGAGTTCATAATGCCTTGACTTTTGCAACTCACACATTCTTCCAAAATCATGGCTTTCTTTATGTGCAATTACCGACCATCACGACCACAGACAGCGAGGGATTCAGTGAAAAGTTCCACGTTACAACCCGTGCCGGCAATTCAGAAGTTCAGAAAATAGAATCAACAGAAGACAAGGAAACTGATGGTGTGAGTCTTGAAACTGTTAAGGCTGCTGCAATAGAGAAAAAAAATATTGTCAATGAACTCGAGAGAAGTGAAAGCAACAGGGAAGCCTTAGCTGCTGCAGTTCAGGATTTAAAGAAGACAAATGAACTTGCATTACAATTAGAAGCCAGAGGAAAACAAAAATCCAAATTGAAGAAGATAAAGTTCACGTTTCTGAAAATTTCTTCCCACAAGAAACTTATCTCACGGTGTCTGGACAACTCCATTTGGAGACCTATGCGTGTGCCCTTGGAAATGTTTACTCATGCGGACCGAGATAGAATCGAGTCGGCAAAACATGCTGCTGAAATGTGGATGGTGGAGGTCGAAATTGCTTTTGCTGAACTCGAGGATGTCATCAACTGTGCTGATGACCTTGTTAAGTTCCTCTCCAAATGGGTGTCGGAGCACTGTCTGGATGATATGAAATTCGTGCAAAAACGAATCGACAAAACGAGCATTCATTGCCTTGAATCAACCATCAAAAGTTCATTTGAGAAGGTCTCTTACACTGCTGCAATAAAAATTCTGAATAAGGTGAATCTTCGAACAGATGCCTCGGCAACCGATCAAAAATCTCAATGCTCGAAAAGGCTTAAATTTGGTGCTGCTTTAACTGATGATCATCTCAGTTATCTTGCTGATGCCTTCTTTAAGAAGCCTGTGATAGTATATAACTACCCAAAAGAAACCAAGCCATTTAATGTGCGACTGAATGATGATGGGAAAACAGTGGCAGCATTTGATGTAGTTCTTCCAAAGGGTGGAGTAGTAATTTCTGGCAGCCAAAAGGAGGAGCGTCTCGATGTATTGTTGACAAGGATGAAGGAGATGGGGCTGCCAAGAGAGCAGTACGAGTGGTACTTGGAGATTCGAAGGCATGGAACAGTGAAGCACTCTGGTTTTAGTTTGGCATTTGATCGTTTCGTTCTGTTTACTACTGGCCTTACTGATATCAAAGATGCTATTCCGTTTCCAAGGAGTCTTGGAAAGGTCAGCCATTAA

Protein sequence

MAAEHPTVFTPVTFSKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFREAAVPGDALPPRAKDVSCIEILQSRIPIVRSLLRMLLETSVHKPPPPLPSTTFLVVNDGSCVASLQVAVESSLHHPGQLMPTGTCILVEGILKRAPAPGKHLVRLEVEKILHVGKVEPEKYPLTQKKLLIDLLRKFSYFRPRTTTVATVMRVHNALTFATHTFFQNHGFLYVQLPTITTTDSEGFSEKFHVTTRAGNSEVQKIESTEDKETDGVSLETVKAAAIEKKNIVNELERSESNREALAAAVQDLKKTNELALQLEARGKQKSKLKKIKFTFLKISSHKKLISRCLDNSIWRPMRVPLEMFTHADRDRIESAKHAAEMWMVEVEIAFAELEDVINCADDLVKFLSKWVSEHCLDDMKFVQKRIDKTSIHCLESTIKSSFEKVSYTAAIKILNKVNLRTDASATDQKSQCSKRLKFGAALTDDHLSYLADAFFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLLTRMKEMGLPREQYEWYLEIRRHGTVKHSGFSLAFDRFVLFTTGLTDIKDAIPFPRSLGKVSH
Homology
[Close]
BLAST of Carg05071 vs. NCBI nr
Match: KAG7025439.1 (Asparagine--tRNA ligase, cytoplasmic 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1187.9 bits (3072), Expect = 0.0e+00
Identity = 608/608 (100.00%), Postives = 608/608 (100.00%), Query Frame = 0 

Query: 1   MAAEHPTVFTPVTFSKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFREAAVP 60
           MAAEHPTVFTPVTFSKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFREAAVP
Sbjct: 1   MAAEHPTVFTPVTFSKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFREAAVP 60

Query: 61  GDALPPRAKDVSCIEILQSRIPIVRSLLRMLLETSVHKPPPPLPSTTFLVVNDGSCVASL 120
           GDALPPRAKDVSCIEILQSRIPIVRSLLRMLLETSVHKPPPPLPSTTFLVVNDGSCVASL
Sbjct: 61  GDALPPRAKDVSCIEILQSRIPIVRSLLRMLLETSVHKPPPPLPSTTFLVVNDGSCVASL 120

Query: 121 QVAVESSLHHPGQLMPTGTCILVEGILKRAPAPGKHLVRLEVEKILHVGKVEPEKYPLTQ 180
           QVAVESSLHHPGQLMPTGTCILVEGILKRAPAPGKHLVRLEVEKILHVGKVEPEKYPLTQ
Sbjct: 121 QVAVESSLHHPGQLMPTGTCILVEGILKRAPAPGKHLVRLEVEKILHVGKVEPEKYPLTQ 180

Query: 181 KKLLIDLLRKFSYFRPRTTTVATVMRVHNALTFATHTFFQNHGFLYVQLPTITTTDSEGF 240
           KKLLIDLLRKFSYFRPRTTTVATVMRVHNALTFATHTFFQNHGFLYVQLPTITTTDSEGF
Sbjct: 181 KKLLIDLLRKFSYFRPRTTTVATVMRVHNALTFATHTFFQNHGFLYVQLPTITTTDSEGF 240

Query: 241 SEKFHVTTRAGNSEVQKIESTEDKETDGVSLETVKAAAIEKKNIVNELERSESNREALAA 300
           SEKFHVTTRAGNSEVQKIESTEDKETDGVSLETVKAAAIEKKNIVNELERSESNREALAA
Sbjct: 241 SEKFHVTTRAGNSEVQKIESTEDKETDGVSLETVKAAAIEKKNIVNELERSESNREALAA 300

Query: 301 AVQDLKKTNELALQLEARGKQKSKLKKIKFTFLKISSHKKLISRCLDNSIWRPMRVPLEM 360
           AVQDLKKTNELALQLEARGKQKSKLKKIKFTFLKISSHKKLISRCLDNSIWRPMRVPLEM
Sbjct: 301 AVQDLKKTNELALQLEARGKQKSKLKKIKFTFLKISSHKKLISRCLDNSIWRPMRVPLEM 360

Query: 361 FTHADRDRIESAKHAAEMWMVEVEIAFAELEDVINCADDLVKFLSKWVSEHCLDDMKFVQ 420
           FTHADRDRIESAKHAAEMWMVEVEIAFAELEDVINCADDLVKFLSKWVSEHCLDDMKFVQ
Sbjct: 361 FTHADRDRIESAKHAAEMWMVEVEIAFAELEDVINCADDLVKFLSKWVSEHCLDDMKFVQ 420

Query: 421 KRIDKTSIHCLESTIKSSFEKVSYTAAIKILNKVNLRTDASATDQKSQCSKRLKFGAALT 480
           KRIDKTSIHCLESTIKSSFEKVSYTAAIKILNKVNLRTDASATDQKSQCSKRLKFGAALT
Sbjct: 421 KRIDKTSIHCLESTIKSSFEKVSYTAAIKILNKVNLRTDASATDQKSQCSKRLKFGAALT 480

Query: 481 DDHLSYLADAFFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEE 540
           DDHLSYLADAFFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEE
Sbjct: 481 DDHLSYLADAFFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEE 540

Query: 541 RLDVLLTRMKEMGLPREQYEWYLEIRRHGTVKHSGFSLAFDRFVLFTTGLTDIKDAIPFP 600
           RLDVLLTRMKEMGLPREQYEWYLEIRRHGTVKHSGFSLAFDRFVLFTTGLTDIKDAIPFP
Sbjct: 541 RLDVLLTRMKEMGLPREQYEWYLEIRRHGTVKHSGFSLAFDRFVLFTTGLTDIKDAIPFP 600

Query: 601 RSLGKVSH 609
           RSLGKVSH
Sbjct: 601 RSLGKVSH 608

[Close]
BLAST of Carg05071 vs. NCBI nr
Match: KAG6593030.1 (Asparagine--tRNA ligase, cytoplasmic 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1040.4 bits (2689), Expect = 6.0e-300
Identity = 562/643 (87.40%), Postives = 571/643 (88.80%), Query Frame = 0 

Query: 1   MAAEHPTVFTPVTFSKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFREAAVP 60
           MAAEHPTVFTPVTFSKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFREAAVP
Sbjct: 1   MAAEHPTVFTPVTFSKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFREAAVP 60

Query: 61  --------GDALPPRAKDVSCIEILQSRIPIVRSLLRML----------LETSVHK--PP 120
                   GDALPPRAKDVSCIEILQSRIPIVRSLLRML          LETSVHK  PP
Sbjct: 61  ADASVRTAGDALPPRAKDVSCIEILQSRIPIVRSLLRMLYGNDLHAGEKLETSVHKPPPP 120

Query: 121 PPLPSTTFLVVNDGSCVASLQVAVESSLHHPGQLMPTGTCILVEGILKRAPAPGKHLVRL 180
           PP P TTFLVVNDGSCVASLQVAVESSLHHPGQLMPTGTCILVEGILKRAPAPGKHLVRL
Sbjct: 121 PPPPPTTFLVVNDGSCVASLQVAVESSLHHPGQLMPTGTCILVEGILKRAPAPGKHLVRL 180

Query: 181 EVEKILHVGKVEPEKYPLTQKKLLIDLLRKFSYFRPRTTTVATVMRVHNALTFATHTFFQ 240
           EVEKILHVGKVEPEKYPLTQKKLLID LRKFSYFRPRTTTVATVMRVHNALTFATHTFFQ
Sbjct: 181 EVEKILHVGKVEPEKYPLTQKKLLIDFLRKFSYFRPRTTTVATVMRVHNALTFATHTFFQ 240

Query: 241 NHGFLYVQLPTITTTDSEGFSEKFHVTTRAGNSEVQKIESTEDKETDGVSLETVKAAAIE 300
           NHGFLYVQLPTITTTDSEGFSEKFHVTTRAGNSEVQKIESTEDKETDGVSLETVKAAAIE
Sbjct: 241 NHGFLYVQLPTITTTDSEGFSEKFHVTTRAGNSEVQKIESTEDKETDGVSLETVKAAAIE 300

Query: 301 KKNIVNELERSESNREALAAAVQDLKKTNELALQLEARGKQKSKLKKIKF---------- 360
           KKNIVNELERSESNREALAAAVQDLKKTNE+ALQLEARGKQKSKLKK K           
Sbjct: 301 KKNIVNELERSESNREALAAAVQDLKKTNEIALQLEARGKQKSKLKKDKVHVSENFFPQE 360

Query: 361 TFLKISS--HKKLISRCLDNSIWRPMRVPLEMFTHADR---DRIESAKHAAEMWMVEVEI 420
           T+L +S   H +  +  L N           +++   R   DRIESAKHAAEMWMVEVEI
Sbjct: 361 TYLTVSGQLHLETYACALGN-----------VYSCGPRFRADRIESAKHAAEMWMVEVEI 420

Query: 421 AFAELEDVINCADDLVKFLSKWVSEHCLDDMKFVQKRIDKTSIHCLESTIKSSFEKVSYT 480
           AFAELEDVINCADDLVKFLSKWVSEHCLDDMKFVQKRIDKTSIHCLESTIKSSFEKVSYT
Sbjct: 421 AFAELEDVINCADDLVKFLSKWVSEHCLDDMKFVQKRIDKTSIHCLESTIKSSFEKVSYT 480

Query: 481 AAIKILNKVNLRTDASATDQKSQCSKRLKFGAALTDDHLSYLADAFFKKPVIVYNYPKET 540
           AAIKIL+K        ATDQKSQC KRLKFGAALTDDHLSYLAD FFKKPVIVYNYPKET
Sbjct: 481 AAIKILDK--------ATDQKSQCLKRLKFGAALTDDHLSYLADVFFKKPVIVYNYPKET 540

Query: 541 KPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLLTRMKEMGLPREQYEWYLEI 600
           KPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLLTRMKEMGLPREQYEWYLEI
Sbjct: 541 KPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLLTRMKEMGLPREQYEWYLEI 600

Query: 601 RRHGTVKHSGFSLAFDRFVLFTTGLTDIKDAIPFPRSLGKVSH 609
           RRHGTVKHSGFSLAFDRFVLFTTGLTDIKDAIPFPRSLGKVSH
Sbjct: 601 RRHGTVKHSGFSLAFDRFVLFTTGLTDIKDAIPFPRSLGKVSH 624

[Close]
BLAST of Carg05071 vs. NCBI nr
Match: XP_022959835.1 (asparagine--tRNA ligase, cytoplasmic 2-like [Cucurbita moschata])

HSP 1 Score: 1035.0 bits (2675), Expect = 2.5e-298
Identity = 559/641 (87.21%), Postives = 570/641 (88.92%), Query Frame = 0 

Query: 1   MAAEHPTVFTPVTFSKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFREAAVP 60
           MAAEHPTVFTPVTFSKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFREAAVP
Sbjct: 1   MAAEHPTVFTPVTFSKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFREAAVP 60

Query: 61  G--------DALPPRAKDVSCIEILQSRIPIVRSLLRML----------LETSVHKPPPP 120
                    DALPPRAKDVSCIEILQSRIPIVRSLLRML          LETSVHKPPPP
Sbjct: 61  ADASVRTACDALPPRAKDVSCIEILQSRIPIVRSLLRMLYGNDLHAGEKLETSVHKPPPP 120

Query: 121 LPSTTFLVVNDGSCVASLQVAVESSLHHPGQLMPTGTCILVEGILKRAPAPGKHLVRLEV 180
           LPSTTFLVVNDGSCVASLQVAVESSLHHPGQLMPTGTCILVEGILKRAPAPGKHLVRLEV
Sbjct: 121 LPSTTFLVVNDGSCVASLQVAVESSLHHPGQLMPTGTCILVEGILKRAPAPGKHLVRLEV 180

Query: 181 EKILHVGKVEPEKYPLTQKKLLIDLLRKFSYFRPRTTTVATVMRVHNALTFATHTFFQNH 240
           EKILHVGKVE EKYPLTQKKLLIDLLRKFSYFRPRTTTVATVMRVHNALTFATHTFFQNH
Sbjct: 181 EKILHVGKVEQEKYPLTQKKLLIDLLRKFSYFRPRTTTVATVMRVHNALTFATHTFFQNH 240

Query: 241 GFLYVQLPTITTTDSEGFSEKFHVTTRAGNSEVQKIESTEDKETDGVSLETVKAAAIEKK 300
           GFLYVQLPTITTTDSEGFSEKFHVTTRAGNSEV+KIESTEDKETDGV+LETVKAAAIEKK
Sbjct: 241 GFLYVQLPTITTTDSEGFSEKFHVTTRAGNSEVKKIESTEDKETDGVNLETVKAAAIEKK 300

Query: 301 NIVNELERSESNREALAAAVQDLKKTNELALQLEARGKQKSKLKKIKF----------TF 360
           NIV ELERSESNREALAAAVQDLKKTNELALQLEARGKQKSKLKK K           T+
Sbjct: 301 NIVKELERSESNREALAAAVQDLKKTNELALQLEARGKQKSKLKKDKVHVSENFFPQETY 360

Query: 361 LKISS--HKKLISRCLDNSIWRPMRVPLEMFTHADR---DRIESAKHAAEMWMVEVEIAF 420
           L +S   H +  +  L N           +++   R   DRIESAKHAAEMWMVEVEIAF
Sbjct: 361 LTVSGQLHLETYACALGN-----------VYSCGPRFRADRIESAKHAAEMWMVEVEIAF 420

Query: 421 AELEDVINCADDLVKFLSKWVSEHCLDDMKFVQKRIDKTSIHCLESTIKSSFEKVSYTAA 480
           AELEDVINCADDLVKFLSKWV EHCLDDMKFVQKRIDKTSIH LESTI SSFEKVSYTAA
Sbjct: 421 AELEDVINCADDLVKFLSKWVLEHCLDDMKFVQKRIDKTSIHRLESTITSSFEKVSYTAA 480

Query: 481 IKILNKVNLRTDASATDQKSQCSKRLKFGAALTDDHLSYLADAFFKKPVIVYNYPKETKP 540
           I+IL+K        ATDQKSQCSKRLKFGAALTDDHLSYLADAFFKKPVIVYNYPKETKP
Sbjct: 481 IEILDK--------ATDQKSQCSKRLKFGAALTDDHLSYLADAFFKKPVIVYNYPKETKP 540

Query: 541 FNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLLTRMKEMGLPREQYEWYLEIRR 600
           FNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLLTRMKEMGLPREQYEWYLEIRR
Sbjct: 541 FNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLLTRMKEMGLPREQYEWYLEIRR 600

Query: 601 HGTVKHSGFSLAFDRFVLFTTGLTDIKDAIPFPRSLGKVSH 609
           HGTVKHSGFSLAFDRFVLFTTGLTDIKDAIPFPRSLGKVSH
Sbjct: 601 HGTVKHSGFSLAFDRFVLFTTGLTDIKDAIPFPRSLGKVSH 622

[Close]
BLAST of Carg05071 vs. NCBI nr
Match: XP_023514428.1 (asparagine--tRNA ligase, cytoplasmic 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 999.2 bits (2582), Expect = 1.5e-287
Identity = 541/641 (84.40%), Postives = 557/641 (86.90%), Query Frame = 0 

Query: 1   MAAEHPTVFTPVTFSKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFREAAVP 60
           MAAEHPTVF PVTFSKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFREA VP
Sbjct: 1   MAAEHPTVFAPVTFSKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFREAVVP 60

Query: 61  --------GDALPPRAKDVSCIEILQSRIPIVRSLLRML----------LETSVHKPPPP 120
                   GDALPPRAKDVSCIEILQSRIPIVRSLLRML          LETSVHKPPPP
Sbjct: 61  ADASVRTAGDALPPRAKDVSCIEILQSRIPIVRSLLRMLYGNDLHAGEKLETSVHKPPPP 120

Query: 121 LPSTTFLVVNDGSCVASLQVAVESSLHHPGQLMPTGTCILVEGILKRAPAPGKHLVRLEV 180
           LPSTTFLVVNDGSCVASLQV VESSLHHPGQLMPTGTCILVEGILKR PAPGKHLVRLEV
Sbjct: 121 LPSTTFLVVNDGSCVASLQVVVESSLHHPGQLMPTGTCILVEGILKRTPAPGKHLVRLEV 180

Query: 181 EKILHVGKVEPEKYPLTQKKLLIDLLRKFSYFRPRTTTVATVMRVHNALTFATHTFFQNH 240
           EKILHVGKVE EKYPLTQKKL +DLLRKFSYFRPRTTTVA+VMRV NALTFATHTFFQNH
Sbjct: 181 EKILHVGKVEQEKYPLTQKKLPMDLLRKFSYFRPRTTTVASVMRVRNALTFATHTFFQNH 240

Query: 241 GFLYVQLPTITTTDSEGFSEKFHVTTRAGNSEVQKIESTEDKETDGVSLETVKAAAIEKK 300
           GFLYVQLPTITTTDSEGFSEKFHVTTRAG SE +KIE TEDKETDGVSLETVKAAA+EK+
Sbjct: 241 GFLYVQLPTITTTDSEGFSEKFHVTTRAGKSEDKKIEPTEDKETDGVSLETVKAAAVEKR 300

Query: 301 NIVNELERSESNREALAAAVQDLKKTNELALQLEARGKQKSKLKKIKF----------TF 360
           NIV ELERSESNREALAAAVQDLKKTNELALQLEARGKQK KLKK K           T+
Sbjct: 301 NIVKELERSESNREALAAAVQDLKKTNELALQLEARGKQKLKLKKDKVHVSETFFPQETY 360

Query: 361 LKISS--HKKLISRCLDNSIWRPMRVPLEMFTHADR---DRIESAKHAAEMWMVEVEIAF 420
           L +S   H +  +  L N           +++   R   DRIESAKHAAEMWMVEVEIAF
Sbjct: 361 LTVSGQLHLETYACALGN-----------VYSCGPRFRADRIESAKHAAEMWMVEVEIAF 420

Query: 421 AELEDVINCADDLVKFLSKWVSEHCLDDMKFVQKRIDKTSIHCLESTIKSSFEKVSYTAA 480
           AELEDVINCADDLVKFLSKWV EHCLDDMKFVQKRIDKTSIH LESTI SSFE+VSYTAA
Sbjct: 421 AELEDVINCADDLVKFLSKWVLEHCLDDMKFVQKRIDKTSIHRLESTITSSFERVSYTAA 480

Query: 481 IKILNKVNLRTDASATDQKSQCSKRLKFGAALTDDHLSYLADAFFKKPVIVYNYPKETKP 540
           I+IL+K        ATDQKSQ  KRLKFGAALTDDHLSYLADA FKKPVIVYNYPKETKP
Sbjct: 481 IEILDK--------ATDQKSQRLKRLKFGAALTDDHLSYLADAIFKKPVIVYNYPKETKP 540

Query: 541 FNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLLTRMKEMGLPREQYEWYLEIRR 600
           FNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLLTRMKEMGLPREQYEWYLEI+R
Sbjct: 541 FNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLLTRMKEMGLPREQYEWYLEIQR 600

Query: 601 HGTVKHSGFSLAFDRFVLFTTGLTDIKDAIPFPRSLGKVSH 609
           HGTVKHSGFSLAFDRFVLFTTGLTDIKD IPFPRSLGKVSH
Sbjct: 601 HGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSLGKVSH 622

[Close]
BLAST of Carg05071 vs. NCBI nr
Match: XP_023004185.1 (asparagine--tRNA ligase, cytoplasmic 2-like [Cucurbita maxima])

HSP 1 Score: 995.3 bits (2572), Expect = 2.2e-286
Identity = 537/641 (83.78%), Postives = 557/641 (86.90%), Query Frame = 0 

Query: 1   MAAEHPTVFTPVTFSKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFREAAVP 60
           MAAEHPT FTPVTFSKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFREAAVP
Sbjct: 1   MAAEHPTAFTPVTFSKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFREAAVP 60

Query: 61  --------GDALPPRAKDVSCIEILQSRIPIVRSLLRML----------LETSVHKPPPP 120
                   GDALP RAKDVSCIEILQSRIPIVRSLLRML          LET VHKPPPP
Sbjct: 61  ADASVRTAGDALPRRAKDVSCIEILQSRIPIVRSLLRMLYGNDLHAGEKLETPVHKPPPP 120

Query: 121 LPSTTFLVVNDGSCVASLQVAVESSLHHPGQLMPTGTCILVEGILKRAPAPGKHLVRLEV 180
           LPSTTFLVVNDGSCVASLQV VESSLHHPGQLMPTGTCILVEGILKRAPAPGKHLVRLEV
Sbjct: 121 LPSTTFLVVNDGSCVASLQVVVESSLHHPGQLMPTGTCILVEGILKRAPAPGKHLVRLEV 180

Query: 181 EKILHVGKVEPEKYPLTQKKLLIDLLRKFSYFRPRTTTVATVMRVHNALTFATHTFFQNH 240
           EKILHVGKVE EKYPLTQKKL +DLLRKFSYFRPRTTTVA+VMR+HNALTFATHTFFQNH
Sbjct: 181 EKILHVGKVEQEKYPLTQKKLPMDLLRKFSYFRPRTTTVASVMRIHNALTFATHTFFQNH 240

Query: 241 GFLYVQLPTITTTDSEGFSEKFHVTTRAGNSEVQKIESTEDKETDGVSLETVKAAAIEKK 300
           GFLYVQLPTITTTDSEGFSEKFHVTTRAG SE +KIE TEDKETDGVSLETVKAAA+EK+
Sbjct: 241 GFLYVQLPTITTTDSEGFSEKFHVTTRAGKSEDKKIEPTEDKETDGVSLETVKAAAVEKR 300

Query: 301 NIVNELERSESNREALAAAVQDLKKTNELALQLEARGKQKSKLKKIKF----------TF 360
           NIV ELERSESNREALAAAVQDLKKTNELALQLEARGKQKS+LKK K           T+
Sbjct: 301 NIVKELERSESNREALAAAVQDLKKTNELALQLEARGKQKSQLKKDKVHISENFFPQETY 360

Query: 361 LKISS--HKKLISRCLDNSIWRPMRVPLEMFTHADR---DRIESAKHAAEMWMVEVEIAF 420
           L +S   H +  +  L N           +++   R   DRI SAKHAAEMWMVEVEIAF
Sbjct: 361 LTVSGQLHLETYACALGN-----------VYSCGPRFRADRIVSAKHAAEMWMVEVEIAF 420

Query: 421 AELEDVINCADDLVKFLSKWVSEHCLDDMKFVQKRIDKTSIHCLESTIKSSFEKVSYTAA 480
           AELEDVINCADDLVKFLSKWV EHCLDDMKFVQKRIDKTS+H LESTI SSFEKVSYTAA
Sbjct: 421 AELEDVINCADDLVKFLSKWVLEHCLDDMKFVQKRIDKTSVHRLESTITSSFEKVSYTAA 480

Query: 481 IKILNKVNLRTDASATDQKSQCSKRLKFGAALTDDHLSYLADAFFKKPVIVYNYPKETKP 540
           ++IL+K        ATDQKSQ  KRLKFGAALTDDHLSYLADA FKKPVIVYNYPKETKP
Sbjct: 481 VEILDK--------ATDQKSQRLKRLKFGAALTDDHLSYLADAIFKKPVIVYNYPKETKP 540

Query: 541 FNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLLTRMKEMGLPREQYEWYLEIRR 600
           FNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEER DVLL+RMKEMGLPREQYEWYLEI+R
Sbjct: 541 FNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERFDVLLSRMKEMGLPREQYEWYLEIQR 600

Query: 601 HGTVKHSGFSLAFDRFVLFTTGLTDIKDAIPFPRSLGKVSH 609
           HGTVKHSGFSLAFDRFVLFTTGLTDIKD IPFPRSLGKVSH
Sbjct: 601 HGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSLGKVSH 622

[Close]
BLAST of Carg05071 vs. ExPASy Swiss-Prot
Match: Q9SW95 (Asparagine--tRNA ligase, cytoplasmic 2 OS=Arabidopsis thaliana OX=3702 GN=SYNC2 PE=1 SV=2)

HSP 1 Score: 506.9 bits (1304), Expect = 3.2e-142
Identity = 300/641 (46.80%), Postives = 406/641 (63.34%), Query Frame = 0 

Query: 11  PVTFSKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFREAAVP---------- 70
           P+T SKYSKRV LKT+LD SD G G   +++VIGGWVKS++ V + +  P          
Sbjct: 20  PITLSKYSKRVELKTLLDRSDRGAGLAGKRVVIGGWVKSARAVKKNSPPPPLPVVAAPSP 79

Query: 71  ---GDALPPRAKDVSCIEILQSRIPIVRSLLRML------------LETSVHKPPPPLPS 130
              GD     A ++ C EI+QS++ I +    +L             E +  K  PP   
Sbjct: 80  SSGGDQAHTTA-NIRCTEIIQSKMNIFKRFFDVLSGGGKTYPIFDKTELAGQKAVPPPEY 139

Query: 131 TTFLVVNDGSCVASLQVAVESSLH--HPGQLMPTGTCILVEGILK-RAPAPGKHLVRLEV 190
             + +++DGS ++SLQV V+S+L      QLM  GTCI+ EG+L+    A  KH++ LE 
Sbjct: 140 VFYFLISDGSSISSLQVVVDSALSTVPATQLMALGTCIVAEGVLRLPLAASAKHVIELEA 199

Query: 191 EKILHVGKVEPEKYPLTQKKLLIDLLRKFSYFRPRTTTVATVMRVHNALTFATHTFFQNH 250
           EK+LHVG V+PEKYPL++K+L + +LR FS+FRPRTTTV +V RVH+ALT A+HTF Q H
Sbjct: 200 EKLLHVGTVDPEKYPLSKKQLPLHMLRDFSHFRPRTTTVGSVTRVHSALTLASHTFLQYH 259

Query: 251 GFLYVQLPTITTTDSEGFSEKFHVTTRAGNSEVQKIESTEDKETDGVSLETVKAAAIEKK 310
           GF YVQ+P ITTT   GF E F VTT  G ++ ++ +    +E DG S++TVKA   EK 
Sbjct: 260 GFQYVQVPVITTT--TGFGEMFRVTTLLGKTDDKEEKKPPVQEKDGFSIDTVKAVIKEKT 319

Query: 311 NIVNELERSESNREALAAAVQDLKKTNELALQLEARGKQKS----KLKKIKFTFLKISSH 370
            +++ L+RS+SNRE + AAV DLKKTN+LA Q+E + K K+    K +K+ F+       
Sbjct: 320 RLIDHLKRSDSNRETVVAAVHDLKKTNDLASQIEMKQKSKTGTLVKPEKLDFS------- 379

Query: 371 KKLISRCLDNSIWRPMRVPLEMFTHA-----------DRDRIESAKHAAEMWMVEVEIAF 430
           K    R  D  +    R  LE +  A             D+I++A+H AE W VE E+AF
Sbjct: 380 KDFFGR--DTYLTASGRFHLESYASALGKVYTFGPRFIADKIDNARHLAEKWNVETEMAF 439

Query: 431 AELEDVINCADDLVKFLSKWVSEHCLDDMKFVQKRIDKTSIHCLESTIKSSFEKVSYTAA 490
           AEL+D ++CAD+  KFL K+V E+  +DMKF+ KR+DKT    LE+T  SS  + SYT  
Sbjct: 440 AELDDAMDCADEYFKFLCKYVLENRDEDMKFISKRVDKTITTRLEATASSSLLRFSYTEV 499

Query: 491 IKILNKVNLRTDASATDQKSQCSKRLKFGAALTDDHLSYLADAFFKKPVIVYNYPKETKP 550
           I +L K        AT  K +   + ++G ALT +HLSYL D  +K PVIV+ YPK  K 
Sbjct: 500 ISLLQK--------ATTTKFE--TKPEWGVALTTEHLSYLTDEIYKGPVIVHTYPKAIKQ 559

Query: 551 FNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLLTRMKEMGLPREQYEWYLEIRR 609
           F VRLNDD KTVAAFD+V+PK GVVI+GSQ EER ++L  R+ E G  RE++EWYL++RR
Sbjct: 560 FYVRLNDDKKTVAAFDLVVPKVGVVITGSQNEERFEILDARIGESGFTREKFEWYLDLRR 619

[Close]
BLAST of Carg05071 vs. ExPASy Swiss-Prot
Match: Q9SW96 (Asparagine--tRNA ligase, cytoplasmic 1 OS=Arabidopsis thaliana OX=3702 GN=SYNC1 PE=1 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 5.1e-108
Identity = 231/603 (38.31%), Postives = 335/603 (55.56%), Query Frame = 0 

Query: 15  SKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFREAAVPGDALPPRAKDVSCI 74
           +++S RVL++T+LD  DGG G   Q + IGGWVKS ++  +                   
Sbjct: 27  AQFSNRVLIRTILDRPDGGAGLAGQTVRIGGWVKSGRDQGKR------------------ 86

Query: 75  EILQSRIPIVRSLLRMLLETSVHKPPPPLPSTTFLVVNDGSCVASLQVAVESSLHHPGQL 134
                                         + +FL VNDGSC A+LQV V+ SL+    L
Sbjct: 87  ------------------------------TFSFLAVNDGSCPANLQVMVDPSLYDVSNL 146

Query: 135 MPTGTCILVEGILKRAPAPGK---HLVRLEVEKILHVGKVEPEKYPLTQKKLLIDLLRKF 194
           + TGTC+ V+G+LK  P  GK     + L V K++ VG V+  KYPL + KL ++ LR  
Sbjct: 147 VATGTCVTVDGVLK-VPPKGKGTQQQIELNVVKVIDVGTVDASKYPLPKTKLTLETLRDV 206

Query: 195 SYFRPRTTTVATVMRVHNALTFATHTFFQNHGFLYVQLPTITTTDSEGFSEKFHVTTRAG 254
            + R RT +++ V R+ NAL FATH+FFQ H FLY+  P ITT+D EG  E F  TT   
Sbjct: 207 LHLRSRTNSISAVARIRNALAFATHSFFQEHSFLYIHTPIITTSDCEGAGEMFQATTLIN 266

Query: 255 NSEVQKIESTEDKETDGVSLETVKAAAIEKKNIVNELERSESNREALAAAVQDLKKTNEL 314
            +E  + +  ++       +E  +   IE+ N+V EL+ +++++EA+ AAV +LK   E 
Sbjct: 267 YTERLEQDLIDNPPPTEADVEAARLIVIERGNVVAELKAAKASKEAITAAVAELKIAKET 326

Query: 315 ALQLEARGKQKSKLKKIKFTFLKISSHKKLISRCLDNSIWRPMRVPL------EMFTHAD 374
              ++ R + +  L K       I   K    R    ++   ++V         ++T   
Sbjct: 327 FAHIDERSRLRPGLPKKDG---NIDYSKDFFGRQAFLTVSGQLQVETYACALSNVYTFGP 386

Query: 375 RDRIE---SAKHAAEMWMVEVEIAFAELEDVINCADDLVKFLSKWVSEHCLDDMKFVQKR 434
             R E   +++H AE WMVE EIAFA+LED +NCA+  VK++  W+ E C  DM+ + K 
Sbjct: 387 TFRAENSHTSRHLAEFWMVEPEIAFADLEDDMNCAEAYVKYMCNWLLEKCYADMELMAKN 446

Query: 435 IDKTSIHCLESTIKSSFEKVSYTAAIKILNKVNLRTDASATDQKSQCSKRLKFGAALTDD 494
            D   I  L+    + F +++YT AI++L +        A  +  +    +++G  L  +
Sbjct: 447 FDSGCIDRLKLVASTPFGRITYTKAIELLEE--------AVAKGKEFDNNVEWGIDLASE 506

Query: 495 HLSYLADAFFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERL 554
           H  YL +  F+KP+IVYNYPK  K F +RLNDD KTVAA DV++PK G +I GSQ+EER 
Sbjct: 507 HERYLTEVLFQKPLIVYNYPKGIKAFYMRLNDDEKTVAAMDVLVPKVGELIGGSQREERY 566

Query: 555 DVLLTRMKEMGLPREQYEWYLEIRRHGTVKHSGFSLAFDRFVLFTTGLTDIKDAIPFPRS 606
           DV+  R++EMGLP E YEWYL++RR+GTVKH GF L F+R +LF TGL +I+D IPFPR 
Sbjct: 567 DVIKKRIEEMGLPIEPYEWYLDLRRYGTVKHCGFGLGFERMILFATGLDNIRDVIPFPRY 569

[Close]
BLAST of Carg05071 vs. ExPASy Swiss-Prot
Match: Q9SSK1 (Asparagine--tRNA ligase, cytoplasmic 3 OS=Arabidopsis thaliana OX=3702 GN=SYNC3 PE=1 SV=1)

HSP 1 Score: 364.4 bits (934), Expect = 2.5e-99
Identity = 225/604 (37.25%), Postives = 330/604 (54.64%), Query Frame = 0 

Query: 15  SKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFREAAVPGDALPPRAKDVSCI 74
           +++S RVL++++L    GG     QK+ IGGWVK+ ++  +                   
Sbjct: 27  AQFSDRVLIRSIL---GGGAKLAGQKVRIGGWVKTGRQQGK------------------- 86

Query: 75  EILQSRIPIVRSLLRMLLETSVHKPPPPLPSTTFLVVNDGSCVASLQVAVESSLHHPGQL 134
                                         +  FL VNDGSC A+LQV V+SSL+   +L
Sbjct: 87  -----------------------------GTFAFLEVNDGSCPANLQVMVDSSLYDLSRL 146

Query: 135 MPTGTCILVEGILKRAPAPGKHL---VRLEVEKILHVGKVEPEKYPLTQKKLLIDLLRKF 194
           + TGTC+ V+G+LK  P  GK L   + L VE ++ VG V+P  YPL + KL  + LR  
Sbjct: 147 VATGTCVTVDGVLK-IPPEGKGLKQSIELSVETVIAVGTVDPTTYPLPKTKLTPEFLRDV 206

Query: 195 SYFRPRTTTVATVMRVHNALTFATHTFFQNHGFLYVQLPTITTTDSEGFSEKFHVTTRAG 254
            + R RT  ++ V R+ NAL FATH+FFQ H FLY+  P ITT+D EG  E F VTT   
Sbjct: 207 LHLRSRTNLISAVARIRNALAFATHSFFQEHSFLYIHTPIITTSDCEGAGEMFQVTTLIN 266

Query: 255 NSEVQKIESTEDKETDGVSLETVKAAAIEKKNIVNELERSESNREALAAAVQDLKKTNEL 314
           ++E  + +  ++       +E  +    E+   V +L+ +++++E + A+V  L      
Sbjct: 267 HTERVEQDLIDNPPPTEADVEAARLIVKERGEAVAQLKVAKASKEEITASVAQLSVAKAS 326

Query: 315 ALQLEARGKQKSKLKKIKFTFLKISSHKKLISRCLDNSIWRPMRVPL------EMFTHAD 374
              +E R + K  L K      KI        R    ++   ++V         ++T   
Sbjct: 327 LAHVEERLRLKPGLPK---NDGKIDYSNDFFGRQAFLTVSGQLQVETYACALSSVYTFGP 386

Query: 375 RDRIE---SAKHAAEMWMVEVEIAFAELEDVINCADDLVKFLSKWVSEHCLDDMKFVQKR 434
             R E   +++H AE WMVE EIAFA++ D +NCA+  VK++ KW+ + C DDM+ + K 
Sbjct: 387 TFRAENSHTSRHLAEFWMVEPEIAFADIHDDMNCAEAYVKYMCKWLMDKCGDDMELMDKN 446

Query: 435 IDKTSIHCLESTIKSSFEKVSYTAAIKILNKVNLRTDASATDQKSQCSKRLKFGAALTDD 494
           +D+     L    K+SF++V+YT AI+ L K       +    K     ++++G  L  +
Sbjct: 447 VDEGCTKRLNMVAKASFKRVTYTEAIERLEK-------AVAQGKVVFDNKVEWGIDLASE 506

Query: 495 HLSYLADA-FFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEER 554
           H  YL +  F +KP+IVYNYPK  K F +RLNDD KTVAA DV++PK G +I GSQ+EER
Sbjct: 507 HERYLTEVEFDQKPIIVYNYPKGIKAFYMRLNDDEKTVAAMDVLVPKVGELIGGSQREER 566

Query: 555 LDVLLTRMKEMGLPREQYEWYLEIRRHGTVKHSGFSLAFDRFVLFTTGLTDIKDAIPFPR 606
            DV+  R++EMGLP E YEWYL++RR+GTVKH GF L F+R + F TG+ +I+D IPFPR
Sbjct: 567 YDVIKQRIEEMGLPMEPYEWYLDLRRYGTVKHCGFGLGFERMIQFATGIDNIRDVIPFPR 568

[Close]
BLAST of Carg05071 vs. ExPASy Swiss-Prot
Match: Q6MEC9 (Asparagine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=asnS PE=3 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 5.0e-79
Identity = 181/502 (36.06%), Postives = 265/502 (52.79%), Query Frame = 0 

Query: 107 TFLVVNDGSCVASLQVAVESSLHHPGQL---MPTGTCILVEGILKRAPAPGKHLVRLEVE 166
           TF+ +NDGS +++ Q+     +    QL   + TG  +   G +  +P   ++L  ++  
Sbjct: 39  TFIEINDGSTLSNFQIIATPDIAGYDQLINQLSTGVSVSAIGTIVESPGKEQNL-EMQAT 98

Query: 167 KILHVGKVEPEKYPLTQKKLLIDLLRKFSYFRPRTTTVATVMRVHNALTFATHTFFQNHG 226
            I  +GK +PE Y L +K+   + LR  ++ RPRT T+  V RV NAL FATH FFQ  G
Sbjct: 99  AITIIGKCDPEVYLLQKKRHTFEFLRSIAHLRPRTNTIGAVTRVRNALAFATHQFFQKRG 158

Query: 227 FLYVQLPTITTTDSEGFSEKFHVTTRAGNSEVQKIESTEDKETDGVSLETVKAAAIEKKN 286
           FLY+  P IT +D EG  + F VTT   N+  +  E   D   D     T    +     
Sbjct: 159 FLYIHTPIITGSDCEGAGKMFQVTTLDQNNPARTPEGRVDYTQDFFGKPTYLTVS----- 218

Query: 287 IVNELERSESNREALAAAVQDLKKTNELALQLEARGKQKSKLKKIKFTFLKISSHKKLIS 346
                   + N E  A A+ D+                        +TF           
Sbjct: 219 -------GQLNGEIYACALSDV------------------------YTF----------- 278

Query: 347 RCLDNSIWRPMRVPLEMFTHADRDRIESAKHAAEMWMVEVEIAFAELEDVINCADDLVKF 406
                  +R              +   +++H AE WM+E E+AFA+L D ++CA+D +K+
Sbjct: 279 ----GPTFR-------------AENSNTSRHLAEFWMIEPEMAFADLNDNMDCAEDYLKY 338

Query: 407 LSKWVSEHCLDDMKFVQKRIDKTSIHCLESTIKSSFEKVSYTAAIKILNKVNLRTDASAT 466
           + K+V ++C +DM+F  K +    I  LE  I +SFE+ SYT A++IL K          
Sbjct: 339 ILKYVLDNCQEDMEFFNKHVATDLISRLEHVINTSFERASYTYAVRILEK---------A 398

Query: 467 DQKSQCSKRLKFGAALTDDHLSYLADAFFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFD 526
           D+K +    +K+G  L  +H  +LA+ FF KPVI+ +YPK+ K F +R N+D KTVAA D
Sbjct: 399 DKKFEYP--VKWGLDLQSEHERFLAEEFFGKPVILTDYPKDIKAFYMRTNEDNKTVAAMD 458

Query: 527 VVLPKGGVVISGSQKEERLDVLLTRMKEMGLPREQYEWYLEIRRHGTVKHSGFSLAFDRF 586
           V++PK G +I GSQ+EERL VL +++KE  LP E+Y WYLE+R+ G+V HSGF   F+R 
Sbjct: 459 VLVPKVGEIIGGSQREERLSVLESKLKEFNLPAEEYWWYLELRKFGSVPHSGFGAGFERL 464

Query: 587 VLFTTGLTDIKDAIPFPRSLGK 606
           V FTTG+ +I+D IPFPR  GK
Sbjct: 519 VQFTTGMENIRDVIPFPRHPGK 464

[Close]
BLAST of Carg05071 vs. ExPASy Swiss-Prot
Match: P52276 (Asparagine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=asnS PE=3 SV=2)

HSP 1 Score: 288.1 bits (736), Expect = 2.3e-76
Identity = 184/507 (36.29%), Postives = 268/507 (52.86%), Query Frame = 0 

Query: 103 LPSTTFLVVNDGSCVASLQVAVESSLHHPGQL---MPTGTCILVEGILKRAPAPGK-HLV 162
           L   +F+ VNDGS +A+LQV ++ SL    +L   + TG  ++VEG  K AP+PGK   V
Sbjct: 31  LKDFSFVEVNDGSSLANLQVVLDGSLADYDRLLSQLQTGAALVVEG--KLAPSPGKGQRV 90

Query: 163 RLEVEKILHVGKVEPEKYPLTQKKLLIDLLRKFSYFRPRTTTVATVMRVHNALTFATHTF 222
            L+  K+  +G  +P  YPL +K+   + LR   + RPRT T+  VMRV NA   A H F
Sbjct: 91  ELKATKLELLGGADPGSYPLQKKRHSFEFLRTIGHLRPRTNTIGAVMRVRNACATAIHQF 150

Query: 223 FQNHGFLYVQLPTITTTDSEGFSEKFHVTTRAGNSEVQKIESTEDKETDGVSLETVKAAA 282
           FQ  GFL+V  P IT +D EG  + F VTT                              
Sbjct: 151 FQERGFLWVHTPIITASDCEGAGDLFKVTTL----------------------------- 210

Query: 283 IEKKNIVNELERSESNREALAAAVQDLKKTNELALQLEARGKQKSKLKKIKFTFLKISSH 342
                   +L+R   N E +  +     K   L +     G+ ++++  + F        
Sbjct: 211 --------DLQRVPKNGEGIDYSQDFFGKQAYLTVS----GQLEAEVMALAFQ------- 270

Query: 343 KKLISRCLDNSIWRPMRVPLEMFTHADRDRIE---SAKHAAEMWMVEVEIAFAELEDVIN 402
                                ++T     R E   +++H AE WMVE E+AF +LE    
Sbjct: 271 --------------------NVYTFGPTFRAENSNTSRHLAEFWMVEPEMAFCDLEGDRQ 330

Query: 403 CADDLVKFLSKWVSEHCLDDMKFVQKRIDKTSIHCLESTIKSSFEKVSYTAAIKILNKVN 462
            A++ +K++ K+V E C +DM+F  +RID T +   ++ I + F  ++Y+ AIK+L K  
Sbjct: 331 WAEEFLKYIFKFVLEKCPEDMEFFDQRIDNTVLATADNIINNEFAWLTYSEAIKLLEK-- 390

Query: 463 LRTDASATDQKSQCSKRLKFGAALTDDHLSYLADAFFKKPVIVYNYPKETKPFNVRLNDD 522
                   DQK +    + +G  L  +H  YLA+  FK+P IV +YPK+ K F +RLNDD
Sbjct: 391 -------ADQKFEYP--VAWGVDLQSEHERYLAETVFKRPTIVTDYPKDIKAFYMRLNDD 450

Query: 523 GKTVAAFDVVLPKGGVVISGSQKEERLDVLLTRMKEMGLPREQYEWYLEIRRHGTVKHSG 582
           GKTVAA D++ PK G +I GSQ+EERLD+L  RM+E G+P E   WYL++RR+G+V H+G
Sbjct: 451 GKTVAAMDILAPKIGEIIGGSQREERLDILTQRMQEQGVPEEDLWWYLDLRRYGSVPHAG 456

Query: 583 FSLAFDRFVLFTTGLTDIKDAIPFPRS 603
           F L F+R V F TG+ +I+D IPFPR+
Sbjct: 511 FGLGFERIVQFMTGMANIRDVIPFPRT 456

[Close]
BLAST of Carg05071 vs. ExPASy TrEMBL
Match: A0A6J1H7G6 (asparagine--tRNA ligase, cytoplasmic 2-like OS=Cucurbita moschata OX=3662 GN=LOC111460779 PE=4 SV=1)

HSP 1 Score: 1035.0 bits (2675), Expect = 1.2e-298
Identity = 559/641 (87.21%), Postives = 570/641 (88.92%), Query Frame = 0 

Query: 1   MAAEHPTVFTPVTFSKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFREAAVP 60
           MAAEHPTVFTPVTFSKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFREAAVP
Sbjct: 1   MAAEHPTVFTPVTFSKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFREAAVP 60

Query: 61  G--------DALPPRAKDVSCIEILQSRIPIVRSLLRML----------LETSVHKPPPP 120
                    DALPPRAKDVSCIEILQSRIPIVRSLLRML          LETSVHKPPPP
Sbjct: 61  ADASVRTACDALPPRAKDVSCIEILQSRIPIVRSLLRMLYGNDLHAGEKLETSVHKPPPP 120

Query: 121 LPSTTFLVVNDGSCVASLQVAVESSLHHPGQLMPTGTCILVEGILKRAPAPGKHLVRLEV 180
           LPSTTFLVVNDGSCVASLQVAVESSLHHPGQLMPTGTCILVEGILKRAPAPGKHLVRLEV
Sbjct: 121 LPSTTFLVVNDGSCVASLQVAVESSLHHPGQLMPTGTCILVEGILKRAPAPGKHLVRLEV 180

Query: 181 EKILHVGKVEPEKYPLTQKKLLIDLLRKFSYFRPRTTTVATVMRVHNALTFATHTFFQNH 240
           EKILHVGKVE EKYPLTQKKLLIDLLRKFSYFRPRTTTVATVMRVHNALTFATHTFFQNH
Sbjct: 181 EKILHVGKVEQEKYPLTQKKLLIDLLRKFSYFRPRTTTVATVMRVHNALTFATHTFFQNH 240

Query: 241 GFLYVQLPTITTTDSEGFSEKFHVTTRAGNSEVQKIESTEDKETDGVSLETVKAAAIEKK 300
           GFLYVQLPTITTTDSEGFSEKFHVTTRAGNSEV+KIESTEDKETDGV+LETVKAAAIEKK
Sbjct: 241 GFLYVQLPTITTTDSEGFSEKFHVTTRAGNSEVKKIESTEDKETDGVNLETVKAAAIEKK 300

Query: 301 NIVNELERSESNREALAAAVQDLKKTNELALQLEARGKQKSKLKKIKF----------TF 360
           NIV ELERSESNREALAAAVQDLKKTNELALQLEARGKQKSKLKK K           T+
Sbjct: 301 NIVKELERSESNREALAAAVQDLKKTNELALQLEARGKQKSKLKKDKVHVSENFFPQETY 360

Query: 361 LKISS--HKKLISRCLDNSIWRPMRVPLEMFTHADR---DRIESAKHAAEMWMVEVEIAF 420
           L +S   H +  +  L N           +++   R   DRIESAKHAAEMWMVEVEIAF
Sbjct: 361 LTVSGQLHLETYACALGN-----------VYSCGPRFRADRIESAKHAAEMWMVEVEIAF 420

Query: 421 AELEDVINCADDLVKFLSKWVSEHCLDDMKFVQKRIDKTSIHCLESTIKSSFEKVSYTAA 480
           AELEDVINCADDLVKFLSKWV EHCLDDMKFVQKRIDKTSIH LESTI SSFEKVSYTAA
Sbjct: 421 AELEDVINCADDLVKFLSKWVLEHCLDDMKFVQKRIDKTSIHRLESTITSSFEKVSYTAA 480

Query: 481 IKILNKVNLRTDASATDQKSQCSKRLKFGAALTDDHLSYLADAFFKKPVIVYNYPKETKP 540
           I+IL+K        ATDQKSQCSKRLKFGAALTDDHLSYLADAFFKKPVIVYNYPKETKP
Sbjct: 481 IEILDK--------ATDQKSQCSKRLKFGAALTDDHLSYLADAFFKKPVIVYNYPKETKP 540

Query: 541 FNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLLTRMKEMGLPREQYEWYLEIRR 600
           FNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLLTRMKEMGLPREQYEWYLEIRR
Sbjct: 541 FNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLLTRMKEMGLPREQYEWYLEIRR 600

Query: 601 HGTVKHSGFSLAFDRFVLFTTGLTDIKDAIPFPRSLGKVSH 609
           HGTVKHSGFSLAFDRFVLFTTGLTDIKDAIPFPRSLGKVSH
Sbjct: 601 HGTVKHSGFSLAFDRFVLFTTGLTDIKDAIPFPRSLGKVSH 622

[Close]
BLAST of Carg05071 vs. ExPASy TrEMBL
Match: A0A6J1KTV9 (asparagine--tRNA ligase, cytoplasmic 2-like OS=Cucurbita maxima OX=3661 GN=LOC111497586 PE=4 SV=1)

HSP 1 Score: 995.3 bits (2572), Expect = 1.1e-286
Identity = 537/641 (83.78%), Postives = 557/641 (86.90%), Query Frame = 0 

Query: 1   MAAEHPTVFTPVTFSKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFREAAVP 60
           MAAEHPT FTPVTFSKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFREAAVP
Sbjct: 1   MAAEHPTAFTPVTFSKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFREAAVP 60

Query: 61  --------GDALPPRAKDVSCIEILQSRIPIVRSLLRML----------LETSVHKPPPP 120
                   GDALP RAKDVSCIEILQSRIPIVRSLLRML          LET VHKPPPP
Sbjct: 61  ADASVRTAGDALPRRAKDVSCIEILQSRIPIVRSLLRMLYGNDLHAGEKLETPVHKPPPP 120

Query: 121 LPSTTFLVVNDGSCVASLQVAVESSLHHPGQLMPTGTCILVEGILKRAPAPGKHLVRLEV 180
           LPSTTFLVVNDGSCVASLQV VESSLHHPGQLMPTGTCILVEGILKRAPAPGKHLVRLEV
Sbjct: 121 LPSTTFLVVNDGSCVASLQVVVESSLHHPGQLMPTGTCILVEGILKRAPAPGKHLVRLEV 180

Query: 181 EKILHVGKVEPEKYPLTQKKLLIDLLRKFSYFRPRTTTVATVMRVHNALTFATHTFFQNH 240
           EKILHVGKVE EKYPLTQKKL +DLLRKFSYFRPRTTTVA+VMR+HNALTFATHTFFQNH
Sbjct: 181 EKILHVGKVEQEKYPLTQKKLPMDLLRKFSYFRPRTTTVASVMRIHNALTFATHTFFQNH 240

Query: 241 GFLYVQLPTITTTDSEGFSEKFHVTTRAGNSEVQKIESTEDKETDGVSLETVKAAAIEKK 300
           GFLYVQLPTITTTDSEGFSEKFHVTTRAG SE +KIE TEDKETDGVSLETVKAAA+EK+
Sbjct: 241 GFLYVQLPTITTTDSEGFSEKFHVTTRAGKSEDKKIEPTEDKETDGVSLETVKAAAVEKR 300

Query: 301 NIVNELERSESNREALAAAVQDLKKTNELALQLEARGKQKSKLKKIKF----------TF 360
           NIV ELERSESNREALAAAVQDLKKTNELALQLEARGKQKS+LKK K           T+
Sbjct: 301 NIVKELERSESNREALAAAVQDLKKTNELALQLEARGKQKSQLKKDKVHISENFFPQETY 360

Query: 361 LKISS--HKKLISRCLDNSIWRPMRVPLEMFTHADR---DRIESAKHAAEMWMVEVEIAF 420
           L +S   H +  +  L N           +++   R   DRI SAKHAAEMWMVEVEIAF
Sbjct: 361 LTVSGQLHLETYACALGN-----------VYSCGPRFRADRIVSAKHAAEMWMVEVEIAF 420

Query: 421 AELEDVINCADDLVKFLSKWVSEHCLDDMKFVQKRIDKTSIHCLESTIKSSFEKVSYTAA 480
           AELEDVINCADDLVKFLSKWV EHCLDDMKFVQKRIDKTS+H LESTI SSFEKVSYTAA
Sbjct: 421 AELEDVINCADDLVKFLSKWVLEHCLDDMKFVQKRIDKTSVHRLESTITSSFEKVSYTAA 480

Query: 481 IKILNKVNLRTDASATDQKSQCSKRLKFGAALTDDHLSYLADAFFKKPVIVYNYPKETKP 540
           ++IL+K        ATDQKSQ  KRLKFGAALTDDHLSYLADA FKKPVIVYNYPKETKP
Sbjct: 481 VEILDK--------ATDQKSQRLKRLKFGAALTDDHLSYLADAIFKKPVIVYNYPKETKP 540

Query: 541 FNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLLTRMKEMGLPREQYEWYLEIRR 600
           FNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEER DVLL+RMKEMGLPREQYEWYLEI+R
Sbjct: 541 FNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERFDVLLSRMKEMGLPREQYEWYLEIQR 600

Query: 601 HGTVKHSGFSLAFDRFVLFTTGLTDIKDAIPFPRSLGKVSH 609
           HGTVKHSGFSLAFDRFVLFTTGLTDIKD IPFPRSLGKVSH
Sbjct: 601 HGTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSLGKVSH 622

[Close]
BLAST of Carg05071 vs. ExPASy TrEMBL
Match: A0A1S3C6Z5 (Asparagine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103497618 PE=3 SV=1)

HSP 1 Score: 875.2 bits (2260), Expect = 1.6e-250
Identity = 475/634 (74.92%), Postives = 519/634 (81.86%), Query Frame = 0 

Query: 1   MAAEHPTVFTPVTFSKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFRE---A 60
           MAA+ PTVF+P+TFSKYS RVLLKT+LD +DGGL FV QKLV+GGWVKSSKEV R+   A
Sbjct: 1   MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAPPA 60

Query: 61  AVPGDALPPRA-KDVSCIEILQSRIPIVRSLLRML----------LETSVHKPPPPLPST 120
             P D+LPP+  KDVSCIEI+QSRIPIVRSLL+ML          L+T VHK  PPLPST
Sbjct: 61  PAPADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHK--PPLPST 120

Query: 121 TFLVVNDGSCVASLQVAVESSLHHPGQLMPTGTCILVEGILKRAPAPGKHLVRLEVEKIL 180
            FL +NDGSCVASLQV VESSLHHP  L+PTGTCILVEG+LK+ P PGKH+VRLEVEKIL
Sbjct: 121 AFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKIL 180

Query: 181 HVGKVEPEKYPLTQKKLLIDLLRKFSYFRPRTTTVATVMRVHNALTFATHTFFQNHGFLY 240
           HVGKVE EKYPL+QKKL +D LRKFSYFRPRTTTVA+V R+ +ALTFA HTFFQN+ FLY
Sbjct: 181 HVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLY 240

Query: 241 VQLPTITTTDSEGFSEKFHVTTRAGNSEVQKIESTEDKETDGVSLETVKAAAIEKKNIVN 300
           VQLPTITTTDSEGFSEKFHVTT AG    +++ESTEDKE DG+SLETVKAA  EK N+V 
Sbjct: 241 VQLPTITTTDSEGFSEKFHVTTLAGKG--KRVESTEDKEPDGLSLETVKAAITEKSNLVK 300

Query: 301 ELERSESNREALAAAVQDLKKTNELALQLEARGKQKSKLKKIKF---------TFLKISS 360
           ELERSESNREAL AAVQDLKKTNELALQLEAR K KSK +K K          T+L +S 
Sbjct: 301 ELERSESNREALVAAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFLPQETYLTVSG 360

Query: 361 --HKKLISRCLDNSIWRPMRVPLEMFTHADR---DRIESAKHAAEMWMVEVEIAFAELED 420
             H +  +  L N           +++   R   D+IESAKHAAEMWMVEVEIAFAEL+D
Sbjct: 361 QLHLETYACALGN-----------VYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKD 420

Query: 421 VINCADDLVKFLSKWVSEHCLDDMKFVQKRIDKTSIHCLESTIKSSFEKVSYTAAIKILN 480
            +NCADDLVKFLSKWV EHC+DDMKFVQKRIDKTSIH LES I  SFEKVSYTAAI+IL 
Sbjct: 421 AMNCADDLVKFLSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAIEILE 480

Query: 481 KVNLRTDASATDQKSQCSKRLKFGAALTDDHLSYLADAFFKKPVIVYNYPKETKPFNVRL 540
           K        ATDQKSQ  K+LKFGAALT DHLSYLAD  FKKPVIVYNYPKETKPFNVRL
Sbjct: 481 K--------ATDQKSQHLKKLKFGAALTADHLSYLADTIFKKPVIVYNYPKETKPFNVRL 540

Query: 541 NDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLLTRMKEMGLPREQYEWYLEIRRHGTVK 600
           NDDGKTVAAFDVVLPKGGVVISGSQKEERLDVL+TRMKEMGLPREQYEWYLEI+RHGTVK
Sbjct: 541 NDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMTRMKEMGLPREQYEWYLEIQRHGTVK 600

Query: 601 HSGFSLAFDRFVLFTTGLTDIKDAIPFPRSLGKV 607
           HSGFSLAFDRFVLFTTGLTDIKD IPFPRS GKV
Sbjct: 601 HSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKV 611

[Close]
BLAST of Carg05071 vs. ExPASy TrEMBL
Match: A0A5D3BD07 (Asparagine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1970G00300 PE=3 SV=1)

HSP 1 Score: 872.5 bits (2253), Expect = 1.1e-249
Identity = 474/638 (74.29%), Postives = 519/638 (81.35%), Query Frame = 0 

Query: 1   MAAEHPTVFTPVTFSKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFRE---- 60
           MAA+ PTVF+P+TFSKYS RVLLKT+LD +DGGL FV QKLV+GGWVKSSKEV R+    
Sbjct: 1   MAAQQPTVFSPITFSKYSNRVLLKTLLDTTDGGLSFVGQKLVVGGWVKSSKEVIRDAPPA 60

Query: 61  ---AAVPGDALPPRA-KDVSCIEILQSRIPIVRSLLRML----------LETSVHKPPPP 120
              A  P D+LPP+  KDVSCIEI+QSRIPIVRSLL+ML          L+T VHK  PP
Sbjct: 61  PAPAPAPADSLPPKLNKDVSCIEIIQSRIPIVRSLLKMLYGNNLHGGEKLDTPVHK--PP 120

Query: 121 LPSTTFLVVNDGSCVASLQVAVESSLHHPGQLMPTGTCILVEGILKRAPAPGKHLVRLEV 180
           LPST FL +NDGSCVASLQV VESSLHHP  L+PTGTCILVEG+LK+ P PGKH+VRLEV
Sbjct: 121 LPSTAFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEV 180

Query: 181 EKILHVGKVEPEKYPLTQKKLLIDLLRKFSYFRPRTTTVATVMRVHNALTFATHTFFQNH 240
           EKILHVGKVE EKYPL+QKKL +D LRKFSYFRPRTTTVA+V R+ +ALTFA HTFFQN+
Sbjct: 181 EKILHVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNN 240

Query: 241 GFLYVQLPTITTTDSEGFSEKFHVTTRAGNSEVQKIESTEDKETDGVSLETVKAAAIEKK 300
            FLYVQLPTITTTDSEGFSEKFHVTT AG    +++ESTEDKE DG+SLETVKAA  EK 
Sbjct: 241 SFLYVQLPTITTTDSEGFSEKFHVTTLAGKG--KRVESTEDKEPDGLSLETVKAAITEKS 300

Query: 301 NIVNELERSESNREALAAAVQDLKKTNELALQLEARGKQKSKLKKIKF---------TFL 360
           N+V ELERSESNREAL AAVQDLKKTN+LALQLEAR K KSK +K K          T+L
Sbjct: 301 NLVKELERSESNREALVAAVQDLKKTNDLALQLEAREKSKSKSRKDKVIPGNFLPQETYL 360

Query: 361 KISS--HKKLISRCLDNSIWRPMRVPLEMFTHADR---DRIESAKHAAEMWMVEVEIAFA 420
            +S   H +  +  L N           +++   R   D+IESAKHAAEMWMVEVEIAFA
Sbjct: 361 TVSGQLHLETYACALGN-----------VYSCGPRFRADKIESAKHAAEMWMVEVEIAFA 420

Query: 421 ELEDVINCADDLVKFLSKWVSEHCLDDMKFVQKRIDKTSIHCLESTIKSSFEKVSYTAAI 480
           EL+D +NCADDLVKFLSKWV EHC+DDMKFVQKRIDKTSIH LES I  SFEKVSYTAAI
Sbjct: 421 ELKDAMNCADDLVKFLSKWVLEHCMDDMKFVQKRIDKTSIHRLESIISCSFEKVSYTAAI 480

Query: 481 KILNKVNLRTDASATDQKSQCSKRLKFGAALTDDHLSYLADAFFKKPVIVYNYPKETKPF 540
           +IL K        ATDQKSQ  K+LKFGAALT DHLSYLAD  FKKPVIVYNYPKETKPF
Sbjct: 481 EILEK--------ATDQKSQHLKKLKFGAALTADHLSYLADTIFKKPVIVYNYPKETKPF 540

Query: 541 NVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLLTRMKEMGLPREQYEWYLEIRRH 600
           NVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVL+TRMKEMGLPREQYEWYLEI+RH
Sbjct: 541 NVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMTRMKEMGLPREQYEWYLEIQRH 600

Query: 601 GTVKHSGFSLAFDRFVLFTTGLTDIKDAIPFPRSLGKV 607
           GTVKHSGFSLAFDRFVLFTTGLTDIKD IPFPRS GKV
Sbjct: 601 GTVKHSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKV 615

[Close]
BLAST of Carg05071 vs. ExPASy TrEMBL
Match: A0A0A0K8G1 (Asparagine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_7G368100 PE=3 SV=1)

HSP 1 Score: 863.6 bits (2230), Expect = 4.9e-247
Identity = 469/636 (73.74%), Postives = 514/636 (80.82%), Query Frame = 0 

Query: 1   MAAEHPTVFTPVTFSKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFRE---A 60
           MAA+ PTVFTP+TFSKYS RVLLKT+LD +DGGL FV QK V+GGWVKSSKEV R+   A
Sbjct: 1   MAAQQPTVFTPITFSKYSNRVLLKTLLDTTDGGLSFVGQKFVVGGWVKSSKEVIRDAPPA 60

Query: 61  AVPGDALPPRA-KDVSCIEILQSRIPIVRSLLRML----------LETSVHKPPPPLPST 120
             P D+LPP+  KDVSCIEI+QSRIPIVRSLL+ML          LET VHK  PPLPST
Sbjct: 61  PAPADSLPPKVNKDVSCIEIIQSRIPIVRSLLKMLYGNSLHDGEKLETPVHK--PPLPST 120

Query: 121 TFLVVNDGSCVASLQVAVESSLHHPGQLMPTGTCILVEGILKRAPAPGKHLVRLEVEKIL 180
            FL +NDGSCVASLQV VESSLHHP  L+PTGTCILVEG+LK+ P PGKH+VRLEVEKIL
Sbjct: 121 AFLAINDGSCVASLQVVVESSLHHPTHLLPTGTCILVEGVLKQVPVPGKHVVRLEVEKIL 180

Query: 181 HVGKVEPEKYPLTQKKLLIDLLRKFSYFRPRTTTVATVMRVHNALTFATHTFFQNHGFLY 240
           HVGKVE EKYPL+QKKL +D LRKFSYFRPRTTTVA+V R+ +ALTFA HTFFQN+ FLY
Sbjct: 181 HVGKVEQEKYPLSQKKLPMDFLRKFSYFRPRTTTVASVTRIRSALTFANHTFFQNNSFLY 240

Query: 241 VQLPTITTTDSEGFSEKFHVTTRAGNSEVQKIESTEDKETDGVSLETVKAAAIEKKNIVN 300
           VQLPTITTTDSEGFSEKFHVTT AG    +++E TEDKE DG+SLETVKAA  EK N+V 
Sbjct: 241 VQLPTITTTDSEGFSEKFHVTTLAGKG--KRVEPTEDKEPDGLSLETVKAAITEKSNLVK 300

Query: 301 ELERSESNREALAAAVQDLKKTNELALQLEARGKQKSKLKKIKF---------TFLKISS 360
           ELERSESNREAL  AVQDLKKTNELALQLEAR K KSK +K K          T+L +S 
Sbjct: 301 ELERSESNREALVTAVQDLKKTNELALQLEAREKSKSKSRKDKVIPGNFFPQETYLTVSG 360

Query: 361 --HKKLISRCLDNSIWRPMRVPLEMFTHADR---DRIESAKHAAEMWMVEVEIAFAELED 420
             H +  +  L N           +++   R   D+IESAKHAAEMWMVEVEIAFAEL+D
Sbjct: 361 QLHLETYACALGN-----------VYSCGPRFRADKIESAKHAAEMWMVEVEIAFAELKD 420

Query: 421 VINCADDLVKFLSKWVSEHCLDDMKFVQKRIDKTSIHCLESTIKSSFEKVSYTAAIKILN 480
            +NCADDLVKFLSKWV EHC+DDMKFVQKRIDK SIH LES I  SFEKVSYT AI+IL 
Sbjct: 421 AMNCADDLVKFLSKWVLEHCMDDMKFVQKRIDKNSIHRLESIISCSFEKVSYTTAIEILE 480

Query: 481 KVNLRTDASATDQKSQCSKRLKFGAALTDDHLSYLADAFFKKPVIVYNYPKETKPFNVRL 540
           K        AT+QKS   K+LKFGAALTDDHLSYLAD  FKKPVIV NYPKETKPFNVRL
Sbjct: 481 K--------ATNQKSPHLKKLKFGAALTDDHLSYLADTIFKKPVIVCNYPKETKPFNVRL 540

Query: 541 NDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLLTRMKEMGLPREQYEWYLEIRRHGTVK 600
           NDDGKTVAAFDVVLPKGGVVISGSQKEERLDVL+TRM+EMGLPREQYEWYLEI+RHG VK
Sbjct: 541 NDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLMTRMREMGLPREQYEWYLEIQRHGIVK 600

Query: 601 HSGFSLAFDRFVLFTTGLTDIKDAIPFPRSLGKVSH 609
           HSGFSLAFDRFVLFTTGLTDIKD IPFPRS GKVS+
Sbjct: 601 HSGFSLAFDRFVLFTTGLTDIKDVIPFPRSFGKVSN 613

[Close]
BLAST of Carg05071 vs. TAIR 10
Match: AT3G07420.1 (asparaginyl-tRNA synthetase 2 )

HSP 1 Score: 506.9 bits (1304), Expect = 2.2e-143
Identity = 300/641 (46.80%), Postives = 406/641 (63.34%), Query Frame = 0 

Query: 11  PVTFSKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFREAAVP---------- 70
           P+T SKYSKRV LKT+LD SD G G   +++VIGGWVKS++ V + +  P          
Sbjct: 20  PITLSKYSKRVELKTLLDRSDRGAGLAGKRVVIGGWVKSARAVKKNSPPPPLPVVAAPSP 79

Query: 71  ---GDALPPRAKDVSCIEILQSRIPIVRSLLRML------------LETSVHKPPPPLPS 130
              GD     A ++ C EI+QS++ I +    +L             E +  K  PP   
Sbjct: 80  SSGGDQAHTTA-NIRCTEIIQSKMNIFKRFFDVLSGGGKTYPIFDKTELAGQKAVPPPEY 139

Query: 131 TTFLVVNDGSCVASLQVAVESSLH--HPGQLMPTGTCILVEGILK-RAPAPGKHLVRLEV 190
             + +++DGS ++SLQV V+S+L      QLM  GTCI+ EG+L+    A  KH++ LE 
Sbjct: 140 VFYFLISDGSSISSLQVVVDSALSTVPATQLMALGTCIVAEGVLRLPLAASAKHVIELEA 199

Query: 191 EKILHVGKVEPEKYPLTQKKLLIDLLRKFSYFRPRTTTVATVMRVHNALTFATHTFFQNH 250
           EK+LHVG V+PEKYPL++K+L + +LR FS+FRPRTTTV +V RVH+ALT A+HTF Q H
Sbjct: 200 EKLLHVGTVDPEKYPLSKKQLPLHMLRDFSHFRPRTTTVGSVTRVHSALTLASHTFLQYH 259

Query: 251 GFLYVQLPTITTTDSEGFSEKFHVTTRAGNSEVQKIESTEDKETDGVSLETVKAAAIEKK 310
           GF YVQ+P ITTT   GF E F VTT  G ++ ++ +    +E DG S++TVKA   EK 
Sbjct: 260 GFQYVQVPVITTT--TGFGEMFRVTTLLGKTDDKEEKKPPVQEKDGFSIDTVKAVIKEKT 319

Query: 311 NIVNELERSESNREALAAAVQDLKKTNELALQLEARGKQKS----KLKKIKFTFLKISSH 370
            +++ L+RS+SNRE + AAV DLKKTN+LA Q+E + K K+    K +K+ F+       
Sbjct: 320 RLIDHLKRSDSNRETVVAAVHDLKKTNDLASQIEMKQKSKTGTLVKPEKLDFS------- 379

Query: 371 KKLISRCLDNSIWRPMRVPLEMFTHA-----------DRDRIESAKHAAEMWMVEVEIAF 430
           K    R  D  +    R  LE +  A             D+I++A+H AE W VE E+AF
Sbjct: 380 KDFFGR--DTYLTASGRFHLESYASALGKVYTFGPRFIADKIDNARHLAEKWNVETEMAF 439

Query: 431 AELEDVINCADDLVKFLSKWVSEHCLDDMKFVQKRIDKTSIHCLESTIKSSFEKVSYTAA 490
           AEL+D ++CAD+  KFL K+V E+  +DMKF+ KR+DKT    LE+T  SS  + SYT  
Sbjct: 440 AELDDAMDCADEYFKFLCKYVLENRDEDMKFISKRVDKTITTRLEATASSSLLRFSYTEV 499

Query: 491 IKILNKVNLRTDASATDQKSQCSKRLKFGAALTDDHLSYLADAFFKKPVIVYNYPKETKP 550
           I +L K        AT  K +   + ++G ALT +HLSYL D  +K PVIV+ YPK  K 
Sbjct: 500 ISLLQK--------ATTTKFE--TKPEWGVALTTEHLSYLTDEIYKGPVIVHTYPKAIKQ 559

Query: 551 FNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERLDVLLTRMKEMGLPREQYEWYLEIRR 609
           F VRLNDD KTVAAFD+V+PK GVVI+GSQ EER ++L  R+ E G  RE++EWYL++RR
Sbjct: 560 FYVRLNDDKKTVAAFDLVVPKVGVVITGSQNEERFEILDARIGESGFTREKFEWYLDLRR 619

[Close]
BLAST of Carg05071 vs. TAIR 10
Match: AT5G56680.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 393.3 bits (1009), Expect = 3.6e-109
Identity = 231/603 (38.31%), Postives = 335/603 (55.56%), Query Frame = 0 

Query: 15  SKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFREAAVPGDALPPRAKDVSCI 74
           +++S RVL++T+LD  DGG G   Q + IGGWVKS ++  +                   
Sbjct: 27  AQFSNRVLIRTILDRPDGGAGLAGQTVRIGGWVKSGRDQGKR------------------ 86

Query: 75  EILQSRIPIVRSLLRMLLETSVHKPPPPLPSTTFLVVNDGSCVASLQVAVESSLHHPGQL 134
                                         + +FL VNDGSC A+LQV V+ SL+    L
Sbjct: 87  ------------------------------TFSFLAVNDGSCPANLQVMVDPSLYDVSNL 146

Query: 135 MPTGTCILVEGILKRAPAPGK---HLVRLEVEKILHVGKVEPEKYPLTQKKLLIDLLRKF 194
           + TGTC+ V+G+LK  P  GK     + L V K++ VG V+  KYPL + KL ++ LR  
Sbjct: 147 VATGTCVTVDGVLK-VPPKGKGTQQQIELNVVKVIDVGTVDASKYPLPKTKLTLETLRDV 206

Query: 195 SYFRPRTTTVATVMRVHNALTFATHTFFQNHGFLYVQLPTITTTDSEGFSEKFHVTTRAG 254
            + R RT +++ V R+ NAL FATH+FFQ H FLY+  P ITT+D EG  E F  TT   
Sbjct: 207 LHLRSRTNSISAVARIRNALAFATHSFFQEHSFLYIHTPIITTSDCEGAGEMFQATTLIN 266

Query: 255 NSEVQKIESTEDKETDGVSLETVKAAAIEKKNIVNELERSESNREALAAAVQDLKKTNEL 314
            +E  + +  ++       +E  +   IE+ N+V EL+ +++++EA+ AAV +LK   E 
Sbjct: 267 YTERLEQDLIDNPPPTEADVEAARLIVIERGNVVAELKAAKASKEAITAAVAELKIAKET 326

Query: 315 ALQLEARGKQKSKLKKIKFTFLKISSHKKLISRCLDNSIWRPMRVPL------EMFTHAD 374
              ++ R + +  L K       I   K    R    ++   ++V         ++T   
Sbjct: 327 FAHIDERSRLRPGLPKKDG---NIDYSKDFFGRQAFLTVSGQLQVETYACALSNVYTFGP 386

Query: 375 RDRIE---SAKHAAEMWMVEVEIAFAELEDVINCADDLVKFLSKWVSEHCLDDMKFVQKR 434
             R E   +++H AE WMVE EIAFA+LED +NCA+  VK++  W+ E C  DM+ + K 
Sbjct: 387 TFRAENSHTSRHLAEFWMVEPEIAFADLEDDMNCAEAYVKYMCNWLLEKCYADMELMAKN 446

Query: 435 IDKTSIHCLESTIKSSFEKVSYTAAIKILNKVNLRTDASATDQKSQCSKRLKFGAALTDD 494
            D   I  L+    + F +++YT AI++L +        A  +  +    +++G  L  +
Sbjct: 447 FDSGCIDRLKLVASTPFGRITYTKAIELLEE--------AVAKGKEFDNNVEWGIDLASE 506

Query: 495 HLSYLADAFFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEERL 554
           H  YL +  F+KP+IVYNYPK  K F +RLNDD KTVAA DV++PK G +I GSQ+EER 
Sbjct: 507 HERYLTEVLFQKPLIVYNYPKGIKAFYMRLNDDEKTVAAMDVLVPKVGELIGGSQREERY 566

Query: 555 DVLLTRMKEMGLPREQYEWYLEIRRHGTVKHSGFSLAFDRFVLFTTGLTDIKDAIPFPRS 606
           DV+  R++EMGLP E YEWYL++RR+GTVKH GF L F+R +LF TGL +I+D IPFPR 
Sbjct: 567 DVIKKRIEEMGLPIEPYEWYLDLRRYGTVKHCGFGLGFERMILFATGLDNIRDVIPFPRY 569

[Close]
BLAST of Carg05071 vs. TAIR 10
Match: AT1G70980.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 364.4 bits (934), Expect = 1.8e-100
Identity = 225/604 (37.25%), Postives = 330/604 (54.64%), Query Frame = 0 

Query: 15  SKYSKRVLLKTVLDCSDGGLGFVDQKLVIGGWVKSSKEVFREAAVPGDALPPRAKDVSCI 74
           +++S RVL++++L    GG     QK+ IGGWVK+ ++  +                   
Sbjct: 27  AQFSDRVLIRSIL---GGGAKLAGQKVRIGGWVKTGRQQGK------------------- 86

Query: 75  EILQSRIPIVRSLLRMLLETSVHKPPPPLPSTTFLVVNDGSCVASLQVAVESSLHHPGQL 134
                                         +  FL VNDGSC A+LQV V+SSL+   +L
Sbjct: 87  -----------------------------GTFAFLEVNDGSCPANLQVMVDSSLYDLSRL 146

Query: 135 MPTGTCILVEGILKRAPAPGKHL---VRLEVEKILHVGKVEPEKYPLTQKKLLIDLLRKF 194
           + TGTC+ V+G+LK  P  GK L   + L VE ++ VG V+P  YPL + KL  + LR  
Sbjct: 147 VATGTCVTVDGVLK-IPPEGKGLKQSIELSVETVIAVGTVDPTTYPLPKTKLTPEFLRDV 206

Query: 195 SYFRPRTTTVATVMRVHNALTFATHTFFQNHGFLYVQLPTITTTDSEGFSEKFHVTTRAG 254
            + R RT  ++ V R+ NAL FATH+FFQ H FLY+  P ITT+D EG  E F VTT   
Sbjct: 207 LHLRSRTNLISAVARIRNALAFATHSFFQEHSFLYIHTPIITTSDCEGAGEMFQVTTLIN 266

Query: 255 NSEVQKIESTEDKETDGVSLETVKAAAIEKKNIVNELERSESNREALAAAVQDLKKTNEL 314
           ++E  + +  ++       +E  +    E+   V +L+ +++++E + A+V  L      
Sbjct: 267 HTERVEQDLIDNPPPTEADVEAARLIVKERGEAVAQLKVAKASKEEITASVAQLSVAKAS 326

Query: 315 ALQLEARGKQKSKLKKIKFTFLKISSHKKLISRCLDNSIWRPMRVPL------EMFTHAD 374
              +E R + K  L K      KI        R    ++   ++V         ++T   
Sbjct: 327 LAHVEERLRLKPGLPK---NDGKIDYSNDFFGRQAFLTVSGQLQVETYACALSSVYTFGP 386

Query: 375 RDRIE---SAKHAAEMWMVEVEIAFAELEDVINCADDLVKFLSKWVSEHCLDDMKFVQKR 434
             R E   +++H AE WMVE EIAFA++ D +NCA+  VK++ KW+ + C DDM+ + K 
Sbjct: 387 TFRAENSHTSRHLAEFWMVEPEIAFADIHDDMNCAEAYVKYMCKWLMDKCGDDMELMDKN 446

Query: 435 IDKTSIHCLESTIKSSFEKVSYTAAIKILNKVNLRTDASATDQKSQCSKRLKFGAALTDD 494
           +D+     L    K+SF++V+YT AI+ L K       +    K     ++++G  L  +
Sbjct: 447 VDEGCTKRLNMVAKASFKRVTYTEAIERLEK-------AVAQGKVVFDNKVEWGIDLASE 506

Query: 495 HLSYLADA-FFKKPVIVYNYPKETKPFNVRLNDDGKTVAAFDVVLPKGGVVISGSQKEER 554
           H  YL +  F +KP+IVYNYPK  K F +RLNDD KTVAA DV++PK G +I GSQ+EER
Sbjct: 507 HERYLTEVEFDQKPIIVYNYPKGIKAFYMRLNDDEKTVAAMDVLVPKVGELIGGSQREER 566

Query: 555 LDVLLTRMKEMGLPREQYEWYLEIRRHGTVKHSGFSLAFDRFVLFTTGLTDIKDAIPFPR 606
            DV+  R++EMGLP E YEWYL++RR+GTVKH GF L F+R + F TG+ +I+D IPFPR
Sbjct: 567 YDVIKQRIEEMGLPMEPYEWYLDLRRYGTVKHCGFGLGFERMIQFATGIDNIRDVIPFPR 568

[Close]
BLAST of Carg05071 vs. TAIR 10
Match: AT4G17300.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 263.5 bits (672), Expect = 4.3e-70
Identity = 176/504 (34.92%), Postives = 268/504 (53.17%), Query Frame = 0 

Query: 105 STTFLVVNDGSCVASLQVAVESSLHHPGQL----MPTGTCILVEGILKRAPAPGKHLVRL 164
           S TF+ +NDGSC+++LQ  + S      Q+    + TG  + V+G +  A    K  V L
Sbjct: 126 SVTFIEINDGSCLSNLQCVMTSDAEGYDQVESGSILTGASVSVQGTIV-ASQGTKQKVEL 185

Query: 165 EVEKILHVGKVEPEKYPLTQKKLLIDLLRKFSYFRPRTTTVATVMRVHNALTFATHTFFQ 224
           +VEKI+ VG+ +   YP+ +K++  + LR  ++ RPRT T   V RV N L +ATH FFQ
Sbjct: 186 KVEKIIVVGECD-SSYPIQKKRVSREFLRTKAHLRPRTNTFGAVARVRNTLAYATHKFFQ 245

Query: 225 NHGFLYVQLPTITTTDSEGFSEKFHVTTRAGNSEVQKIESTEDKETDGVSLETVKAAAIE 284
             GF++V  P IT +D EG  E+F VTT   +S        E+ +T   ++   K   I+
Sbjct: 246 ESGFVWVASPIITASDCEGAGEQFCVTTLIPSSH-------ENTDTSIDAIPKTKGGLID 305

Query: 285 KKNIVNELERSESNREALAAAVQD-LKKTNELALQLEARGKQKSKLKKIKFTFLKISSHK 344
                                 QD   K   L +  +  G+  +      +TF       
Sbjct: 306 WS--------------------QDFFGKPAFLTVSGQLNGETYATALSDVYTF------- 365

Query: 345 KLISRCLDNSIWRPMRVPLEMFTHADRDRIESAKHAAEMWMVEVEIAFAELEDVINCADD 404
                      +R              +   +++H AE WM+E E+AFA+L+D + CA  
Sbjct: 366 --------GPTFR-------------AENSNTSRHLAEFWMIEPELAFADLDDDMACATA 425

Query: 405 LVKFLSKWVSEHCLDDMKFVQKRIDKTSIHCLESTIKSSFEKVSYTAAIKILNKVNLRTD 464
            ++++ K+V ++C +DM+F    I+K  I  L    +  F ++ YT AI+IL K N + D
Sbjct: 426 YLQYVVKYVLDNCKEDMEFFDTWIEKGIIRRLSDVAEKEFLQLGYTDAIEILLKANKKFD 485

Query: 465 ASATDQKSQCSKRLKFGAALTDDHLSYLA-DAFFKKPVIVYNYPKETKPFNVRLNDDGKT 524
                        +K+G  L  +H  Y+  +AF  +PVI+ +YPKE K F +R NDDGKT
Sbjct: 486 FP-----------VKWGLDLQSEHERYITEEAFGGRPVIIRDYPKEIKAFYMRENDDGKT 545

Query: 525 VAAFDVVLPKGGVVISGSQKEERLDVLLTRMKEMGLPREQYEWYLEIRRHGTVKHSGFSL 584
           VAA D+++P+ G +I GSQ+EERL+VL  R+ E+ L +E Y WYL++RR+G+V H+GF L
Sbjct: 546 VAAMDMLVPRIGELIGGSQREERLEVLEARLDELKLNKESYWWYLDLRRYGSVPHAGFGL 561

Query: 585 AFDRFVLFTTGLTDIKDAIPFPRS 603
            F+R V F TG+ +I+D IPFPR+
Sbjct: 606 GFERLVQFVTGIDNIRDVIPFPRT 561

[Close]
BLAST of Carg05071 vs. TAIR 10
Match: AT1G68420.1 (Class II aaRS and biotin synthetases superfamily protein )

HSP 1 Score: 60.1 bits (144), Expect = 7.2e-09
Identity = 28/73 (38.36%), Postives = 50/73 (68.49%), Query Frame = 0 

Query: 359 EMFTHADRDRIE---SAKHAAEMWMVEVEIAFAELEDVINCADDLVKFLSKWVSEHCLDD 418
           +++T A   R E   +++H AE WMVEVE+AFA +E+ +NC++ +VK +   + E C DD
Sbjct: 3   DVYTFAPTFRAEKSHTSRHLAEFWMVEVELAFAGVEEAMNCSEAVVKDMCTTLLEKCRDD 62

Query: 419 MKFVQKRIDKTSI 429
           M+++ +++D+  I
Sbjct: 63  MEYMVEKVDEFCI 75

The following BLAST results are available for this feature:
BLAST of Carg05071 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG7025439.10.0e+00100.00Asparagine--tRNA ligase, cytoplasmic 2 [Cucurbita argyrosperma subsp. argyrosper... [more]
KAG6593030.16.0e-30087.40Asparagine--tRNA ligase, cytoplasmic 2, partial [Cucurbita argyrosperma subsp. s... [more]
XP_022959835.12.5e-29887.21asparagine--tRNA ligase, cytoplasmic 2-like [Cucurbita moschata][more]
XP_023514428.11.5e-28784.40asparagine--tRNA ligase, cytoplasmic 2-like [Cucurbita pepo subsp. pepo][more]
XP_023004185.12.2e-28683.78asparagine--tRNA ligase, cytoplasmic 2-like [Cucurbita maxima][more]
BLAST of Carg05071 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9SW953.2e-14246.80Asparagine--tRNA ligase, cytoplasmic 2 OS=Arabidopsis thaliana OX=3702 GN=SYNC2 ... [more]
Q9SW965.1e-10838.31Asparagine--tRNA ligase, cytoplasmic 1 OS=Arabidopsis thaliana OX=3702 GN=SYNC1 ... [more]
Q9SSK12.5e-9937.25Asparagine--tRNA ligase, cytoplasmic 3 OS=Arabidopsis thaliana OX=3702 GN=SYNC3 ... [more]
Q6MEC95.0e-7936.06Asparagine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 G... [more]
P522762.3e-7636.29Asparagine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=11117... [more]
BLAST of Carg05071 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1H7G61.2e-29887.21asparagine--tRNA ligase, cytoplasmic 2-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1KTV91.1e-28683.78asparagine--tRNA ligase, cytoplasmic 2-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A1S3C6Z51.6e-25074.92Asparagine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103497618 PE=3 SV=1[more]
A0A5D3BD071.1e-24974.29Asparagine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A0A0K8G14.9e-24773.74Asparagine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_7G368100 PE=3 SV=1[more]
BLAST of Carg05071 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G07420.12.2e-14346.80asparaginyl-tRNA synthetase 2 [more]
AT5G56680.13.6e-10938.31Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT1G70980.11.8e-10037.25Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT4G17300.14.3e-7034.92Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT1G68420.17.2e-0938.36Class II aaRS and biotin synthetases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 274..318
NoneNo IPR availableGENE3D3.30.930.10Bira Bifunctional Protein; Domain 2coord: 361..607
e-value: 8.8E-55
score: 188.3
NoneNo IPR availableGENE3D3.30.930.10Bira Bifunctional Protein; Domain 2coord: 182..346
e-value: 2.0E-9
score: 38.9
NoneNo IPR availablePANTHERPTHR22594ASPARTYL/LYSYL-TRNA SYNTHETASEcoord: 16..607
NoneNo IPR availablePANTHERPTHR22594:SF36ASPARAGINE--TRNA LIGASE, CYTOPLASMIC 2-RELATEDcoord: 16..607
NoneNo IPR availableSUPERFAMILY55681Class II aaRS and biotin synthetasescoord: 186..601
IPR004364Aminoacyl-tRNA synthetase, class II (D/K/N)PFAMPF00152tRNA-synt_2coord: 368..602
e-value: 2.2E-39
score: 135.4
IPR004364Aminoacyl-tRNA synthetase, class II (D/K/N)PFAMPF00152tRNA-synt_2coord: 187..251
e-value: 3.4E-8
score: 32.9

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg05071-RACarg05071-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006418 tRNA aminoacylation for protein translation
biological_process GO:0006421 asparaginyl-tRNA aminoacylation
cellular_component GO:0009507 chloroplast
cellular_component GO:0005739 mitochondrion
molecular_function GO:0004816 asparagine-tRNA ligase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0003677 DNA binding
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0000166 nucleotide binding