Carg04995 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg04995
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	sphingosine-1-phosphate lyase
Location	Carg_Chr08: 898358 .. 904414 (-)
RNA-Seq Expression	Carg04995
Synteny	Carg04995

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTCTGCTGTCAAGTCGTTTTTGGTGGAATTTAGAGCCTCTGCCAATGGTTTCCTGTCGAAGTACGAGCCGCTGGCTCTCGTTTTTGGTCCTCTTTTCGTCGTACTCTTGGCTCGGTTGCTTCAGTCATTTCTGGATCTTGTTTATGAAAGAGGACTTAAAGCCACCGTCCTATCGTTCTTCATGGCTACGATTAAGTATGACTTTCTCTGCTGAAGTTGTTAGGTGTTGTGTTAATTTGATTTTGTTAGAAATGTGGTGATGTTCTTGGTTGATTGTGTCTGTGGAAACAGGTTAATTCCAGTGGTGAAACGTCGGATTGATTCTGAGAAGCAGAAGGTTAGTTTTCGTGTTTATGGATTTGTTTTTGATTGTTATGCGTTCTTTAAACGACATGCAGTTACATTTATGAGTTGCTTTTATGTTATAGTATCCATGTCGATTTTTTTTTGGAATTGCATCCATTTCGATTTAACAGAATGAGATTATATTCTATACTGTATTCGTTGTATTTAAATGAGTAACATTAATGATATGGTATGTGTCGAGAATTTATTGTTAGGGCTGCTGGATCAGTACAGAGCCTTAAATTCTTATTTCTTGGTGGCGATTTTAGTTGGTCAAACTTTGACCCTGTGCGTTGATTCTTCTTTCTTTTTTTCTGTGGAAAATTTGGTACTGCGTTTGCTTCATTTCGGTATCCGTCTTCGATTTAGATGGTCACCTCGGAAAAACTGGCAGTTAAGAACTCGTCGAATTTCTCAGAGTAGGAAATATCAGCACCATGTTTTTCCAGGTTTCTCATCATGTACATATATGGAGACATCCCGATATCTTTTGAAGGAGAAAATAAGAAACTTCAGACCTAATTGAAATTTTTTGAATTTTTTAACTGATAACGTACTTGCTTCTATTTTTATTGGATTTTAACTATATTGATTGTGGAACTTGGAAACTTTGTATTGAATAAATGAAAAGAGATTAGTGCTAAAGAATACGAACTTGAAGAGAGTGAAAAAAAAAAAAAAAACATAAAGGAGATATCAAGAGTACATATAATTACACTAAAAGAGAGAAAACTGAAAATATCAAACTTTGAGGCAATCTTGAGATCTGAACTAAACTTGGAACTTTGAATAACCAACCAACTGAGAGAAAGGGACACTGAAAGATTCGATGAAGAAACTTGCAAAGAAAGAACCCCTAAGGCCAACACAAAAGAAAGCCCGCTGAAATAACACAAAAATACACCAATGTTCTTCAACAAAAACCTGCTTGAAAACACTTGAGAGCCTCTGAGAACAAAAGCAACCAAAGACAAGAAAACAGCAATCACTGTGGACCTTATGGTATTGTACAGTCTAGTCATGCAATAGATTAAGTCTTTGATGTCTCTGTCCCTTGGTGCTTTAGTCAATATCTTTATTGAAGTTATTCAGGAGATGCTTTTACATTTGGATTATGCCTATAGATTGACGGATGGATTACCGGTCAAAATCGGTACAACCAAACACTGGCCATGTTGGTTTCATGAGTTCAGATACCTGAGTAGGCTGTACCCACTTCACAGAAATCGTCTTCCTCACTACGCTCTATTTCCATTTTAGGCTAAAGAGAAAAGCTACCATCTTTGCTGACTCCGCTTTCTTCTCTTGTAGCTAAATAGTACGTGGTTTTTGTGACTTTGGTGATGATGTTTTGTTGAATAAACAGGTTGTGGATAAACTACAATCCAGCGGTCAATCTAAAAGAGAGAGTTGGAGGACTGAGTTGCCAAGGGCAGGCCTGGGATCTGAAGTCATTGTGAAAATGAAAAATGAGAAACAAAATGATGTGGTTTGGCAAGGCAAATGCTCGGGTACAGTGTATGTTTTGAAAATGTTTGCATAGGCACATTTACCCATTCAGTTATACTTGCATTAGAATGTGATAACACTGAAATTGATTTTTTTTTTCCTTGCATTAGAATGAAATTGTCTCTTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTGTCCACAATGCTTATGAGTATTATTCATTTCTGAGCAGCTACATTGGAGGAACGGAATCTGAAGGTCATTTTTCTCTCATAAACGAGGCATGCTCAATGTTAGTAAGTTATAGCTGATAGATTACTTTTATGTTTCATTCAAAGTCTACTGAAGTGCTGAAGTCACTTAACTATCACGTAACTGGTGCAATTTCTATTTAAGTGGAGTTTCAACTATCGTGTTCTTATTCTACTTTATTGGAGAGCCTCCATATCGGCAACTAATAAAACTCCCAGAGGAACGTAACCTGTTTCATAGATCTTTAACAATTCGCCTTGGGCTTTCTGGGTAACTTTTTCAGGTTTGCACATACAAATCCTTTGCATTTGGATGTATTTCAAAGTGTCGTGCGGTTTGAAGCTGAAGTGGTGGCCATGACTGCTGCTCTTCTTGGCAGTAAAGTGGAGGCTTCTGGAGGGCAAATCTGCGGCAACATGACGTCGGGAGGAACTGAAAGCATTCTATTGGCTGTGAAATCAACTCGTGACTATATGAAATCCAAGAAGGGAATTACAAAACCTGAAATGTATACGCCGTGTTTTCATGCATGTTCTGTTCGATACGTTTCTTGTTGACCCTTAATTTAATTTAGTTTTCCCTCAATGTTCAGGATCATACCTGAGTCTGCACATTCTGCATATGATAAGGCTGCTCAATATTTTAATATCAAGTTGTGGCGTGTCCCTGTAGATAAGGAATTCAAAGCTGATGTCAAAGCTATTAGAAAATTTATCAACAGGAACACTATCTTGGTATCCATATACTTTTATTCTCTTCTTATACTTGTATTTACATAAACTCTCATTCTCTATCATCTTTTTCGCATTTTTATCCACAATTTTGTTTATTGCCCCATTTTTGTTGATTTTTTTTTTCTTTCAAATTCCATTATCAAGAACTAAGAAGTATGATACTGTTGCTTTTGCTTATTCAGAAAAGCTGCTTCACCCTATTATAGTATTTGTATTTGTATGTTACCTGCTCATTTGTGTCTTTTCCCTTGTGCTCTTCATATTGCAGATTGTTGGATCTGCACCTGGTTTTCCCCATGGAATCATTGATCCGATTGTTGTATGTTCATTTTCTCTTGTATCTTTTAGTTGTTTTCTATATGCCGGGACAAAGACCATCAATCGTAACTTAAATGGCATTTCTCAAGTAATTTCTGATCAGGAGGATCTTGCAATATAATTGCAGGAACTTGGGAAATTAGCTTCTAGCTATGGTATTTGTCTTCATGTTGACTTTTGTCTTGGCGGGTTTGTGTTACCGTTTGCGCGTAAGCTTGGGTAAGGCTGTTTGGGCTTTCTCTGAACAAAAAATTTACCACTATTAACATTCTTAATGTAACACATCAATACTCTAACAGGAATGCTTTTGCAAAACATTTGTAAATTATGAAATGATTTTTCACTAACCTCTTTCTCTATGATATCCTATGAAAGAATATAACAATATATCCCCCCTTCCCACCCATATGTGTGGGATTATATTTTCTAGTTCAGCACACCAAACTTGCAGTGATTAGTTATCAAGTGGAAATTACATTTAGCGACGAGTGTTGCAATTATTATTCATTATCGGTGTTTCTCTGGCTATCTGTGATATGAAATTGTCTTATTACTCCTGTCAGGTACCCAATTCCACCTTGTGATTTTTCAGTTAAAGGAGTGACATCAATATCTGCGGATGTACATAAATATGGATTAGCCCCCAAAGGAACAAGTATAGTGTTGTACAGAAATCATGAGATTAGAAAGGTAGTTTCTTTTACCAAATCACTTAAATTTTATTTGATTAGCCCCAAACTAACCTCCCAATGGCCTCCTAGATCTTGTCTGCTGAAGTGTTTGATTGCTTAATGCTTATATGATCATCTTTTTTTTCTTTCTTATAGTAAATGTATTATGTAAACTAGAGGATGTTTCCAGATGCTTTGAAGTTAAATAGCTAAGAACTAGATATTAGAACAAACGACTCTCAAAACGTTAAAAGGTTAATGGATGTAATCGAGCTGCATTCTGAAACCCTATCTTTTCTATCATTGAAAATATATTTTATGTTATTTCTGATTTCATTTGGCTTTATGGTTGTTGATCGGTTGGTGTTCTGCCTGTTCCTGCTTTGCTTGATTTCGACAGCATCAATTTGTTGCTGGTAAGTCTACTTATCCTTGTTACTGGAACGTTGGATTTTTTCATGCCTCGAGTTTAAATCAAGCTATTAATCATTACTAATAGCTATTGGGCTCCATGATTGTCATCAATGTTTAGTTTTATTTTGGGATGTATTTACGAAATTGTGAGAAATAAAATATGTTTTCTTTGTCACGTTTCATTAGTGGAAACCTTTATGGAGACTTTTAAAGACTTAGGCTAATGCAATTGTATACTTATCCCTTGTATGGAGCACAGTCCTTGTACTGAAAAAAATGCTCTCATTCTTATTTTTGTTGTGGTCTTGAATTCAAGGAAATGACATTTCCCTTTTCAATTATCGTCTTTTGTAGTAAGTGAATGGTCTGGTGGGCTTTACGTCTCTCCAACAATAGCTGGAAGCAGACCTGGTGGTTTGATTGCTGGGGCTTGGGCAGCAATGATGTCTTTGGGAGAAGAAGGTATCTAAGACATGAATGTAACGAATACTCATTGTTATCCTCATATTTTGTTAAGTACTTGGCTTTGTGCTCTTTGTGATTCGATTTCTTTCCATAGGATATTTGCAGAACACAAAAGAGATCATGGAAGTGTCAAAGAAGATACATAAAGGGTATCATATTCATCTGTTTAGCCTGGAAGAATTGCAAGGTGTTTTTCTCTTTCAAAAACCTTACTTTTTGTGCAGGGTAAACGAAATTCCTGAGTTATTTGTCGTCGGAAAGCCAGACATGACAATTGTAGCATTTGGATCTACTGTTATTGACATTTTTGAAGTCAATGACATTATGTCATCGAAAGGCTGGCATTTGAATGCTTTGCAAAGACCTAGCAGGTGAGCTTTCTCATTCAGCTTTTATTTATACATATATAAGAAACCAAGAACGAGGTTCCAATCCAATAAAATAAGACCTTACAAATAATTACAAAATAACTTCTTCGCCCAAAGAGAAATTTTGGAGTCTAGATGAGGGACCAAATGTCGCAAGGGCCCTCTCAACACCCTAAAGATTCTTTTGTCTCTCTCCCACAAAAACCTCCCTTCTCCAATCGTATCAAGGTTGCCATATTGGAAGGTGCGGTTGGATCACCTCCTTTTCAGGAAGAGCTAATAGTTGTTGAGTATTTTAGTTTGACGTTGCTTCACACTAAAAAATAAGTGACTACGTCTGAGTTAAACTTGAAGATGGAAGTCTTCTTTCGTTTCTCGACGGAATACGGAATAAGGAGGGAGCATAAAGGATATAATATTTGAAAGAATTTAACCAACCAGAACTCAATTAGTCGTTAAAGCCTGGATTTTCCTTGTCCCCCTTAAGCTGTACATATGTTTTAGGTATGAAACTCAAAATCCTTGAGTGGTTAAAAGAAAGTTTGATGGCTTTGATTCATTGAAACAAAATTTTAAAGTTCACTCCAAAATTTTAATGTTCTCAAAGACTAATCATCTCTCATATTGATTCAGCATTCATATCTGTGTGACGCTTCAACATGTATCAATAGTCGACAACTTTCTCCAGGATTTGCAAGAATCTGTCAACACTGTAAGCTTATTTCTGCAAGAATGTTCTTACTCCATCAGAGGCAATTTCACTAATATGCATATTTTCTGGTTGTGACTTATGAACTTACTTATATGTAATTATATATTTCAGGTCAAACAAAATCCAGGGCCTATTACTGGAGGATTAGCACCAATATATGGTGCTGCAGAGCGAATGCCAGACAGAGACATGGTTCAGGATTTGCTCATAAGCTACATGGATGGTACATGCTAGCAGGCAGTGTGCAGGCCTCATTTGCTCGCACTGGTTTGTTAAAATATAAAATTTGATTTTAT

mRNA sequence

ATGGATTCTGCTGTCAAGTCGTTTTTGGTGGAATTTAGAGCCTCTGCCAATGGTTTCCTGTCGAAGTACGAGCCGCTGGCTCTCGTTTTTGGTCCTCTTTTCGTCGTACTCTTGGCTCGGTTGCTTCAGTCATTTCTGGATCTTGTTTATGAAAGAGGACTTAAAGCCACCGTCCTATCGTTCTTCATGGCTACGATTAAGTTAATTCCAGTGGTGAAACGTCGGATTGATTCTGAGAAGCAGAAGGTTGTGGATAAACTACAATCCAGCGGTCAATCTAAAAGAGAGAGTTGGAGGACTGAGTTGCCAAGGGCAGGCCTGGGATCTGAAGTCATTGTGAAAATGAAAAATGAGAAACAAAATGATGTGGTTTGGCAAGGCAAATGCTCGGGTACAGTGTTTGCACATACAAATCCTTTGCATTTGGATGTATTTCAAAGTGTCGTGCGGTTTGAAGCTGAAGTGGTGGCCATGACTGCTGCTCTTCTTGGCAGTAAAGTGGAGGCTTCTGGAGGGCAAATCTGCGGCAACATGACGTCGGGAGGAACTGAAAGCATTCTATTGGCTGTGAAATCAACTCGTGACTATATGAAATCCAAGAAGGGAATTACAAAACCTGAAATGATCATACCTGAGTCTGCACATTCTGCATATGATAAGGCTGCTCAATATTTTAATATCAAGTTGTGGCGTGTCCCTGTAGATAAGGAATTCAAAGCTGATGTCAAAGCTATTAGAAAATTTATCAACAGGAACACTATCTTGATTGTTGGATCTGCACCTGGTTTTCCCCATGGAATCATTGATCCGATTGTTGAACTTGGGAAATTAGCTTCTAGCTATGGTATTTGTCTTCATGTTGACTTTTGTCTTGGCGGGTTTGTGTTACCGTTTGCGCGTAAGCTTGGGTACCCAATTCCACCTTGTGATTTTTCAGTTAAAGGAGTGACATCAATATCTGCGGATGTACATAAATATGGATTAGCCCCCAAAGGAACAATAAGTGAATGGTCTGGTGGGCTTTACGTCTCTCCAACAATAGCTGGAAGCAGACCTGGTGGTTTGATTGCTGGGGCTTGGGCAGCAATGATGTCTTTGGGAGAAGAAGGATATTTGCAGAACACAAAAGAGATCATGGAAGTGTCAAAGAAGATACATAAAGGGGTAAACGAAATTCCTGAGTTATTTGTCGTCGGAAAGCCAGACATGACAATTGTAGCATTTGGATCTACTGTTATTGACATTTTTGAAGTCAATGACATTATGTCATCGAAAGGCTGGCATTTGAATGCTTTGCAAAGACCTAGCAGCATTCATATCTGTGTGACGCTTCAACATGTATCAATAGTCGACAACTTTCTCCAGGATTTGCAAGAATCTGTCAACACTGTCAAACAAAATCCAGGGCCTATTACTGGAGGATTAGCACCAATATATGGTGCTGCAGAGCGAATGCCAGACAGAGACATGGTTCAGGATTTGCTCATAAGCTACATGGATGGTACATGCTAGCAGGCAGTGTGCAGGCCTCATTTGCTCGCACTGGTTTGTTAAAATATAAAATTTGATTTTAT

Coding sequence (CDS)

ATGGATTCTGCTGTCAAGTCGTTTTTGGTGGAATTTAGAGCCTCTGCCAATGGTTTCCTGTCGAAGTACGAGCCGCTGGCTCTCGTTTTTGGTCCTCTTTTCGTCGTACTCTTGGCTCGGTTGCTTCAGTCATTTCTGGATCTTGTTTATGAAAGAGGACTTAAAGCCACCGTCCTATCGTTCTTCATGGCTACGATTAAGTTAATTCCAGTGGTGAAACGTCGGATTGATTCTGAGAAGCAGAAGGTTGTGGATAAACTACAATCCAGCGGTCAATCTAAAAGAGAGAGTTGGAGGACTGAGTTGCCAAGGGCAGGCCTGGGATCTGAAGTCATTGTGAAAATGAAAAATGAGAAACAAAATGATGTGGTTTGGCAAGGCAAATGCTCGGGTACAGTGTTTGCACATACAAATCCTTTGCATTTGGATGTATTTCAAAGTGTCGTGCGGTTTGAAGCTGAAGTGGTGGCCATGACTGCTGCTCTTCTTGGCAGTAAAGTGGAGGCTTCTGGAGGGCAAATCTGCGGCAACATGACGTCGGGAGGAACTGAAAGCATTCTATTGGCTGTGAAATCAACTCGTGACTATATGAAATCCAAGAAGGGAATTACAAAACCTGAAATGATCATACCTGAGTCTGCACATTCTGCATATGATAAGGCTGCTCAATATTTTAATATCAAGTTGTGGCGTGTCCCTGTAGATAAGGAATTCAAAGCTGATGTCAAAGCTATTAGAAAATTTATCAACAGGAACACTATCTTGATTGTTGGATCTGCACCTGGTTTTCCCCATGGAATCATTGATCCGATTGTTGAACTTGGGAAATTAGCTTCTAGCTATGGTATTTGTCTTCATGTTGACTTTTGTCTTGGCGGGTTTGTGTTACCGTTTGCGCGTAAGCTTGGGTACCCAATTCCACCTTGTGATTTTTCAGTTAAAGGAGTGACATCAATATCTGCGGATGTACATAAATATGGATTAGCCCCCAAAGGAACAATAAGTGAATGGTCTGGTGGGCTTTACGTCTCTCCAACAATAGCTGGAAGCAGACCTGGTGGTTTGATTGCTGGGGCTTGGGCAGCAATGATGTCTTTGGGAGAAGAAGGATATTTGCAGAACACAAAAGAGATCATGGAAGTGTCAAAGAAGATACATAAAGGGGTAAACGAAATTCCTGAGTTATTTGTCGTCGGAAAGCCAGACATGACAATTGTAGCATTTGGATCTACTGTTATTGACATTTTTGAAGTCAATGACATTATGTCATCGAAAGGCTGGCATTTGAATGCTTTGCAAAGACCTAGCAGCATTCATATCTGTGTGACGCTTCAACATGTATCAATAGTCGACAACTTTCTCCAGGATTTGCAAGAATCTGTCAACACTGTCAAACAAAATCCAGGGCCTATTACTGGAGGATTAGCACCAATATATGGTGCTGCAGAGCGAATGCCAGACAGAGACATGGTTCAGGATTTGCTCATAAGCTACATGGATGGTACATGCTAG

Protein sequence

MDSAVKSFLVEFRASANGFLSKYEPLALVFGPLFVVLLARLLQSFLDLVYERGLKATVLSFFMATIKLIPVVKRRIDSEKQKVVDKLQSSGQSKRESWRTELPRAGLGSEVIVKMKNEKQNDVVWQGKCSGTVFAHTNPLHLDVFQSVVRFEAEVVAMTAALLGSKVEASGGQICGNMTSGGTESILLAVKSTRDYMKSKKGITKPEMIIPESAHSAYDKAAQYFNIKLWRVPVDKEFKADVKAIRKFINRNTILIVGSAPGFPHGIIDPIVELGKLASSYGICLHVDFCLGGFVLPFARKLGYPIPPCDFSVKGVTSISADVHKYGLAPKGTISEWSGGLYVSPTIAGSRPGGLIAGAWAAMMSLGEEGYLQNTKEIMEVSKKIHKGVNEIPELFVVGKPDMTIVAFGSTVIDIFEVNDIMSSKGWHLNALQRPSSIHICVTLQHVSIVDNFLQDLQESVNTVKQNPGPITGGLAPIYGAAERMPDRDMVQDLLISYMDGTC

Homology

[Close]

BLAST of Carg04995 vs. NCBI nr
Match: KAG7025365.1 (Sphingosine-1-phosphate lyase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 998.8 bits (2581), Expect = 1.7e-287
Identity = 503/503 (100.00%), Postives = 503/503 (100.00%), Query Frame = 0


Query: 1   MDSAVKSFLVEFRASANGFLSKYEPLALVFGPLFVVLLARLLQSFLDLVYERGLKATVLS 60
           MDSAVKSFLVEFRASANGFLSKYEPLALVFGPLFVVLLARLLQSFLDLVYERGLKATVLS
Sbjct: 1   MDSAVKSFLVEFRASANGFLSKYEPLALVFGPLFVVLLARLLQSFLDLVYERGLKATVLS 60

Query: 61  FFMATIKLIPVVKRRIDSEKQKVVDKLQSSGQSKRESWRTELPRAGLGSEVIVKMKNEKQ 120
           FFMATIKLIPVVKRRIDSEKQKVVDKLQSSGQSKRESWRTELPRAGLGSEVIVKMKNEKQ
Sbjct: 61  FFMATIKLIPVVKRRIDSEKQKVVDKLQSSGQSKRESWRTELPRAGLGSEVIVKMKNEKQ 120

Query: 121 NDVVWQGKCSGTVFAHTNPLHLDVFQSVVRFEAEVVAMTAALLGSKVEASGGQICGNMTS 180
           NDVVWQGKCSGTVFAHTNPLHLDVFQSVVRFEAEVVAMTAALLGSKVEASGGQICGNMTS
Sbjct: 121 NDVVWQGKCSGTVFAHTNPLHLDVFQSVVRFEAEVVAMTAALLGSKVEASGGQICGNMTS 180

Query: 181 GGTESILLAVKSTRDYMKSKKGITKPEMIIPESAHSAYDKAAQYFNIKLWRVPVDKEFKA 240
           GGTESILLAVKSTRDYMKSKKGITKPEMIIPESAHSAYDKAAQYFNIKLWRVPVDKEFKA
Sbjct: 181 GGTESILLAVKSTRDYMKSKKGITKPEMIIPESAHSAYDKAAQYFNIKLWRVPVDKEFKA 240

Query: 241 DVKAIRKFINRNTILIVGSAPGFPHGIIDPIVELGKLASSYGICLHVDFCLGGFVLPFAR 300
           DVKAIRKFINRNTILIVGSAPGFPHGIIDPIVELGKLASSYGICLHVDFCLGGFVLPFAR
Sbjct: 241 DVKAIRKFINRNTILIVGSAPGFPHGIIDPIVELGKLASSYGICLHVDFCLGGFVLPFAR 300

Query: 301 KLGYPIPPCDFSVKGVTSISADVHKYGLAPKGTISEWSGGLYVSPTIAGSRPGGLIAGAW 360
           KLGYPIPPCDFSVKGVTSISADVHKYGLAPKGTISEWSGGLYVSPTIAGSRPGGLIAGAW
Sbjct: 301 KLGYPIPPCDFSVKGVTSISADVHKYGLAPKGTISEWSGGLYVSPTIAGSRPGGLIAGAW 360

Query: 361 AAMMSLGEEGYLQNTKEIMEVSKKIHKGVNEIPELFVVGKPDMTIVAFGSTVIDIFEVND 420
           AAMMSLGEEGYLQNTKEIMEVSKKIHKGVNEIPELFVVGKPDMTIVAFGSTVIDIFEVND
Sbjct: 361 AAMMSLGEEGYLQNTKEIMEVSKKIHKGVNEIPELFVVGKPDMTIVAFGSTVIDIFEVND 420

Query: 421 IMSSKGWHLNALQRPSSIHICVTLQHVSIVDNFLQDLQESVNTVKQNPGPITGGLAPIYG 480
           IMSSKGWHLNALQRPSSIHICVTLQHVSIVDNFLQDLQESVNTVKQNPGPITGGLAPIYG
Sbjct: 421 IMSSKGWHLNALQRPSSIHICVTLQHVSIVDNFLQDLQESVNTVKQNPGPITGGLAPIYG 480

Query: 481 AAERMPDRDMVQDLLISYMDGTC 504
           AAERMPDRDMVQDLLISYMDGTC
Sbjct: 481 AAERMPDRDMVQDLLISYMDGTC 503

[Close]

BLAST of Carg04995 vs. NCBI nr
Match: KAG6592955.1 (Sphingosine-1-phosphate lyase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 975.7 bits (2521), Expect = 1.5e-280
Identity = 502/540 (92.96%), Postives = 503/540 (93.15%), Query Frame = 0


Query: 1   MDSAVKSFLVEFRASANGFLSKYEPLALVFGPLFVVLLARLLQSFLDLVYERGLKATVLS 60
           MDSAVKSFLVEFRASANGFLSKYEPLALVFGPLFVVLLARLLQSFLDLVYERGLKATVLS
Sbjct: 1   MDSAVKSFLVEFRASANGFLSKYEPLALVFGPLFVVLLARLLQSFLDLVYERGLKATVLS 60

Query: 61  FFMATIKLIPVVKRRIDSEKQKVVDKLQSSGQSKRESWRTELPRAGLGSEVIVKMKNEKQ 120
           FFMATIKLIPVVKRRIDSEKQKVVDKLQSSGQSKRESWRTELPRAGLGSEVIVKMKNEKQ
Sbjct: 61  FFMATIKLIPVVKRRIDSEKQKVVDKLQSSGQSKRESWRTELPRAGLGSEVIVKMKNEKQ 120

Query: 121 NDVVWQGKCSGTV--------------------FAHTNPLHLDVFQSVVRFEAEVVAMTA 180
           NDVVWQGKCSGTV                    FAHTNPLHLDVFQSVVRFEAEVVAMTA
Sbjct: 121 NDVVWQGKCSGTVYIGGTESEGHFSLINEACSMFAHTNPLHLDVFQSVVRFEAEVVAMTA 180

Query: 181 ALLGSKVEASGGQICGNMTSGGTESILLAVKSTRDYMKSKKGITKPEMIIPESAHSAYDK 240
           ALLGSKVEASGGQICGNMTSGGTESILLAVKSTRDYMKSKKGITKPEMIIPESAHSAYDK
Sbjct: 181 ALLGSKVEASGGQICGNMTSGGTESILLAVKSTRDYMKSKKGITKPEMIIPESAHSAYDK 240

Query: 241 AAQYFNIKLWRVPVDKEFKADVKAIRKFINRNTILIVGSAPGFPHGIIDPIVELGKLASS 300
           AAQYFNIKLWRVPVDKEFKADVKAIRKFINRNTILIVGSAPGFPHGIIDPIVELGKLASS
Sbjct: 241 AAQYFNIKLWRVPVDKEFKADVKAIRKFINRNTILIVGSAPGFPHGIIDPIVELGKLASS 300

Query: 301 YGICLHVDFCLGGFVLPFARKLGYPIPPCDFSVKGVTSISADVHKYGLAPKGT------- 360
           YGICLHVDFCLGGFVLPFARKLGYPIPPCDFSVKGVTSISADVHKYGLAPKGT       
Sbjct: 301 YGICLHVDFCLGGFVLPFARKLGYPIPPCDFSVKGVTSISADVHKYGLAPKGTSIVLYRN 360

Query: 361 ----------ISEWSGGLYVSPTIAGSRPGGLIAGAWAAMMSLGEEGYLQNTKEIMEVSK 420
                     +SEWSGGLYVSPTIAGSRPGGLIAGAWAAMMSLGEEGYLQNTKEIMEVSK
Sbjct: 361 HEIRKHQFVAVSEWSGGLYVSPTIAGSRPGGLIAGAWAAMMSLGEEGYLQNTKEIMEVSK 420

Query: 421 KIHKGVNEIPELFVVGKPDMTIVAFGSTVIDIFEVNDIMSSKGWHLNALQRPSSIHICVT 480
           KIHKGVNEIPELFVVGKPDMTIVAFGSTVIDIFEVNDIMSSKGWHLNALQRPSSIHICVT
Sbjct: 421 KIHKGVNEIPELFVVGKPDMTIVAFGSTVIDIFEVNDIMSSKGWHLNALQRPSSIHICVT 480

Query: 481 LQHVSIVDNFLQDLQESVNTVKQNPGPITGGLAPIYGAAERMPDRDMVQDLLISYMDGTC 504
           LQHVSIVDNFLQDLQESVNTVKQNPGPITGGLAPIYGAAERMPDRDMVQDLLISYMDGTC
Sbjct: 481 LQHVSIVDNFLQDLQESVNTVKQNPGPITGGLAPIYGAAERMPDRDMVQDLLISYMDGTC 540

[Close]

BLAST of Carg04995 vs. NCBI nr
Match: XP_022960048.1 (sphingosine-1-phosphate lyase [Cucurbita moschata])

HSP 1 Score: 975.3 bits (2520), Expect = 2.0e-280
Identity = 501/540 (92.78%), Postives = 503/540 (93.15%), Query Frame = 0


Query: 1   MDSAVKSFLVEFRASANGFLSKYEPLALVFGPLFVVLLARLLQSFLDLVYERGLKATVLS 60
           MDSAVKSFLVEFRASANGFLSKYEPLALVFGPLFV+LLARLLQSFLDLVYERGLKATVLS
Sbjct: 1   MDSAVKSFLVEFRASANGFLSKYEPLALVFGPLFVILLARLLQSFLDLVYERGLKATVLS 60

Query: 61  FFMATIKLIPVVKRRIDSEKQKVVDKLQSSGQSKRESWRTELPRAGLGSEVIVKMKNEKQ 120
           FFMATIKLIPVVKRRIDSEKQKVVDKLQSSGQSKRESWRTELPRAGLGSEVIVKMKNEKQ
Sbjct: 61  FFMATIKLIPVVKRRIDSEKQKVVDKLQSSGQSKRESWRTELPRAGLGSEVIVKMKNEKQ 120

Query: 121 NDVVWQGKCSGTV--------------------FAHTNPLHLDVFQSVVRFEAEVVAMTA 180
           NDVVWQGKCSGTV                    FAHTNPLHLDVFQSVVRFEAEVVAMTA
Sbjct: 121 NDVVWQGKCSGTVYIGGTESEGHFSLINEACSMFAHTNPLHLDVFQSVVRFEAEVVAMTA 180

Query: 181 ALLGSKVEASGGQICGNMTSGGTESILLAVKSTRDYMKSKKGITKPEMIIPESAHSAYDK 240
           ALLGSKVEASGGQICGNMTSGGTESILLAVKSTRDYMKSKKGITKPEMIIPESAHSAYDK
Sbjct: 181 ALLGSKVEASGGQICGNMTSGGTESILLAVKSTRDYMKSKKGITKPEMIIPESAHSAYDK 240

Query: 241 AAQYFNIKLWRVPVDKEFKADVKAIRKFINRNTILIVGSAPGFPHGIIDPIVELGKLASS 300
           AAQYFNIKLWRVPVDKEFKADVKAIRKFINRNTILIVGSAPGFPHGIIDPIVELGKLASS
Sbjct: 241 AAQYFNIKLWRVPVDKEFKADVKAIRKFINRNTILIVGSAPGFPHGIIDPIVELGKLASS 300

Query: 301 YGICLHVDFCLGGFVLPFARKLGYPIPPCDFSVKGVTSISADVHKYGLAPKGT------- 360
           YGICLHVDFCLGGFVLPFARKLGYPIPPCDFSVKGVTSISADVHKYGLAPKGT       
Sbjct: 301 YGICLHVDFCLGGFVLPFARKLGYPIPPCDFSVKGVTSISADVHKYGLAPKGTSIVLYRN 360

Query: 361 ----------ISEWSGGLYVSPTIAGSRPGGLIAGAWAAMMSLGEEGYLQNTKEIMEVSK 420
                     +SEWSGGLYVSPTIAGSRPGGLIAGAWAAMMSLGEEGYLQNTKEIMEVSK
Sbjct: 361 HEIRKHQFVAVSEWSGGLYVSPTIAGSRPGGLIAGAWAAMMSLGEEGYLQNTKEIMEVSK 420

Query: 421 KIHKGVNEIPELFVVGKPDMTIVAFGSTVIDIFEVNDIMSSKGWHLNALQRPSSIHICVT 480
           KIHKGVNEIPELFVVGKPDMTIVAFGSTVIDIFEVNDIMSSKGWHLNALQRPSSIHICVT
Sbjct: 421 KIHKGVNEIPELFVVGKPDMTIVAFGSTVIDIFEVNDIMSSKGWHLNALQRPSSIHICVT 480

Query: 481 LQHVSIVDNFLQDLQESVNTVKQNPGPITGGLAPIYGAAERMPDRDMVQDLLISYMDGTC 504
           LQHVSIVDNFLQDLQESVNTVKQNPGPITGGLAPIYGAAERMPDRDMVQDLLISYMDGTC
Sbjct: 481 LQHVSIVDNFLQDLQESVNTVKQNPGPITGGLAPIYGAAERMPDRDMVQDLLISYMDGTC 540

[Close]

BLAST of Carg04995 vs. NCBI nr
Match: XP_023514914.1 (sphingosine-1-phosphate lyase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 972.6 bits (2513), Expect = 1.3e-279
Identity = 499/540 (92.41%), Postives = 502/540 (92.96%), Query Frame = 0


Query: 1   MDSAVKSFLVEFRASANGFLSKYEPLALVFGPLFVVLLARLLQSFLDLVYERGLKATVLS 60
           MDSAVKSFLVEFRASANGFLSKYEPLALVFGPLFV+LLARLLQSFLDLVYERGLKAT +S
Sbjct: 1   MDSAVKSFLVEFRASANGFLSKYEPLALVFGPLFVILLARLLQSFLDLVYERGLKATFIS 60

Query: 61  FFMATIKLIPVVKRRIDSEKQKVVDKLQSSGQSKRESWRTELPRAGLGSEVIVKMKNEKQ 120
           FFMATIKLIPVVKRRIDSEKQKVVDKLQSSGQSKRESWRTELPRAGLGSEVIVKMKNEKQ
Sbjct: 61  FFMATIKLIPVVKRRIDSEKQKVVDKLQSSGQSKRESWRTELPRAGLGSEVIVKMKNEKQ 120

Query: 121 NDVVWQGKCSGTV--------------------FAHTNPLHLDVFQSVVRFEAEVVAMTA 180
           NDVVWQGKCSGTV                    FAHTNPLHLDVFQSVVRFEAEVVAMTA
Sbjct: 121 NDVVWQGKCSGTVYIGGTESEGHFSLINEACSMFAHTNPLHLDVFQSVVRFEAEVVAMTA 180

Query: 181 ALLGSKVEASGGQICGNMTSGGTESILLAVKSTRDYMKSKKGITKPEMIIPESAHSAYDK 240
           ALLGSKVEASGGQICGNMTSGGTESILLAVKSTRDYMKSKKGITKPEMIIPESAHSAYDK
Sbjct: 181 ALLGSKVEASGGQICGNMTSGGTESILLAVKSTRDYMKSKKGITKPEMIIPESAHSAYDK 240

Query: 241 AAQYFNIKLWRVPVDKEFKADVKAIRKFINRNTILIVGSAPGFPHGIIDPIVELGKLASS 300
           AAQYFNIKLWRVPVDKEFKADVKAIRKFINRNTILIVGSAPGFPHGIIDPIVELGKLASS
Sbjct: 241 AAQYFNIKLWRVPVDKEFKADVKAIRKFINRNTILIVGSAPGFPHGIIDPIVELGKLASS 300

Query: 301 YGICLHVDFCLGGFVLPFARKLGYPIPPCDFSVKGVTSISADVHKYGLAPKGT------- 360
           YGICLHVDFCLGGFVLPFARKLGYPIPPCDFSVKGVTSISADVHKYGLAPKGT       
Sbjct: 301 YGICLHVDFCLGGFVLPFARKLGYPIPPCDFSVKGVTSISADVHKYGLAPKGTSIVLYRN 360

Query: 361 ----------ISEWSGGLYVSPTIAGSRPGGLIAGAWAAMMSLGEEGYLQNTKEIMEVSK 420
                     +SEWSGGLYVSPTIAGSRPGGLIAGAWAAMMSLGEEGYLQNTKEIMEVSK
Sbjct: 361 HEIRKHQFVAVSEWSGGLYVSPTIAGSRPGGLIAGAWAAMMSLGEEGYLQNTKEIMEVSK 420

Query: 421 KIHKGVNEIPELFVVGKPDMTIVAFGSTVIDIFEVNDIMSSKGWHLNALQRPSSIHICVT 480
           KIHKGVNEIPELFVVGKPDMTIVAFGSTVIDIFEVNDIMSSKGWHLNALQRPSSIHICVT
Sbjct: 421 KIHKGVNEIPELFVVGKPDMTIVAFGSTVIDIFEVNDIMSSKGWHLNALQRPSSIHICVT 480

Query: 481 LQHVSIVDNFLQDLQESVNTVKQNPGPITGGLAPIYGAAERMPDRDMVQDLLISYMDGTC 504
           LQHVSIVDNFLQDLQESVNTVKQNPGPITGGLAPIYGAAERMPDRDMVQDLLISYMDGTC
Sbjct: 481 LQHVSIVDNFLQDLQESVNTVKQNPGPITGGLAPIYGAAERMPDRDMVQDLLISYMDGTC 540

[Close]

BLAST of Carg04995 vs. NCBI nr
Match: XP_023005114.1 (sphingosine-1-phosphate lyase [Cucurbita maxima])

HSP 1 Score: 959.9 bits (2480), Expect = 8.6e-276
Identity = 495/540 (91.67%), Postives = 498/540 (92.22%), Query Frame = 0


Query: 1   MDSAVKSFLVEFRASANGFLSKYEPLALVFGPLFVVLLARLLQSFLDLVYERGLKATVLS 60
           MDSAVKSFLVEFRASANGFLSKYEPLALVFGPLFV LLARLLQSFLDLVYERGLKAT LS
Sbjct: 1   MDSAVKSFLVEFRASANGFLSKYEPLALVFGPLFVTLLARLLQSFLDLVYERGLKATFLS 60

Query: 61  FFMATIKLIPVVKRRIDSEKQKVVDKLQSSGQSKRESWRTELPRAGLGSEVIVKMKNEKQ 120
           FFMATIKLIPVVKRRIDSEKQKVVDKLQSSGQSKRESWRTELPRAGLGSEVIVKMKNEKQ
Sbjct: 61  FFMATIKLIPVVKRRIDSEKQKVVDKLQSSGQSKRESWRTELPRAGLGSEVIVKMKNEKQ 120

Query: 121 NDVVWQGKCSGTV--------------------FAHTNPLHLDVFQSVVRFEAEVVAMTA 180
           NDVVWQGKCSGTV                    FAHTNPLHLDVFQSVVRFEAEVVAMTA
Sbjct: 121 NDVVWQGKCSGTVYIGGTESEGHFSLINEACSMFAHTNPLHLDVFQSVVRFEAEVVAMTA 180

Query: 181 ALLGSKVEASGGQICGNMTSGGTESILLAVKSTRDYMKSKKGITKPEMIIPESAHSAYDK 240
           ALLGSKVEASGGQICGNMTSGGTESILLAVKS+RDYMKSKKGITKPEMIIPESAHSAYDK
Sbjct: 181 ALLGSKVEASGGQICGNMTSGGTESILLAVKSSRDYMKSKKGITKPEMIIPESAHSAYDK 240

Query: 241 AAQYFNIKLWRVPVDKEFKADVKAIRKFINRNTILIVGSAPGFPHGIIDPIVELGKLASS 300
           AAQYFNIKLWRVPVD EFKADVKAIRKFINRNTILIVGSAPGFPHGIIDPIVELGKLASS
Sbjct: 241 AAQYFNIKLWRVPVDTEFKADVKAIRKFINRNTILIVGSAPGFPHGIIDPIVELGKLASS 300

Query: 301 YGICLHVDFCLGGFVLPFARKLGYPIPPCDFSVKGVTSISADVHKYGLAPKGT------- 360
            GICLHVDFCLGGFVLPFA KLGYPIPPCDFSVKGVTSISADVHKYGLAPKGT       
Sbjct: 301 SGICLHVDFCLGGFVLPFACKLGYPIPPCDFSVKGVTSISADVHKYGLAPKGTSVVLYRN 360

Query: 361 ----------ISEWSGGLYVSPTIAGSRPGGLIAGAWAAMMSLGEEGYLQNTKEIMEVSK 420
                     +SEWSGGLYVSPTIAGSRPGGLIAGAWAAMMSLGEEGYLQNTKEIMEVSK
Sbjct: 361 HEIRKHQFVAVSEWSGGLYVSPTIAGSRPGGLIAGAWAAMMSLGEEGYLQNTKEIMEVSK 420

Query: 421 KIHKGVNEIPELFVVGKPDMTIVAFGSTVIDIFEVNDIMSSKGWHLNALQRPSSIHICVT 480
           KIHKGVNEIPELFVVGKPDMTIVAFGSTVIDIFEVNDIMSSKGWHLNALQRPSSIHICVT
Sbjct: 421 KIHKGVNEIPELFVVGKPDMTIVAFGSTVIDIFEVNDIMSSKGWHLNALQRPSSIHICVT 480

Query: 481 LQHVSIVDNFLQDLQESVNTVKQNPGPITGGLAPIYGAAERMPDRDMVQDLLISYMDGTC 504
           LQHVSIVD+FLQDLQESVNTVKQNPGPITGGLAPIYGAAERMPDRDMVQDLLISYMDGTC
Sbjct: 481 LQHVSIVDDFLQDLQESVNTVKQNPGPITGGLAPIYGAAERMPDRDMVQDLLISYMDGTC 540

[Close]

BLAST of Carg04995 vs. ExPASy Swiss-Prot
Match: Q9C509 (Sphingosine-1-phosphate lyase OS=Arabidopsis thaliana OX=3702 GN=DPL1 PE=1 SV=1)

HSP 1 Score: 747.3 bits (1928), Expect = 1.1e-214
Identity = 375/535 (70.09%), Postives = 434/535 (81.12%), Query Frame = 0


Query: 3   SAVKSFLVEFRASANGFLSKYEPLALVFGPLFVVLLARLLQSFLDLVYERGLKATVLSFF 62
           S++KS L++ R S N  LS++EPL L+  PL  + LA+++ S   +V+E+GLKA ++ F 
Sbjct: 7   SSMKSMLIQARGSLNSRLSEFEPLVLLLVPLVSLFLAQIIGSVFGVVHEKGLKACLIGFI 66

Query: 63  MATIKLIPVVKRRIDSEKQKVVDKLQSSGQSKRESWRTELPRAGLGSEVIVKMKNEKQND 122
           M  +K+IP V+  ID+EKQKVVD+LQS   SK+++    LP  GLG EV+ KM+NEK+ND
Sbjct: 67  MGLLKMIPGVQNYIDAEKQKVVDQLQSGSSSKKKNKTEVLPVKGLGVEVLEKMENEKRND 126

Query: 123 VVWQGKCSGTV--------------------FAHTNPLHLDVFQSVVRFEAEVVAMTAAL 182
            +WQGKCSGTV                    FAHTNPLH+DVFQSVVRFE+EVVAMTAAL
Sbjct: 127 AIWQGKCSGTVYIGGAESEGHFSLINQACSMFAHTNPLHIDVFQSVVRFESEVVAMTAAL 186

Query: 183 LGSKVEASGGQICGNMTSGGTESILLAVKSTRDYMKSKKGITKPEMIIPESAHSAYDKAA 242
           LGSK  ASGGQICGNMTSGGTESI+LAVKS+RDYMK KKGIT+PEMIIPES HSAYDKAA
Sbjct: 187 LGSKETASGGQICGNMTSGGTESIVLAVKSSRDYMKYKKGITRPEMIIPESGHSAYDKAA 246

Query: 243 QYFNIKLWRVPVDKEFKADVKAIRKFINRNTILIVGSAPGFPHGIIDPIVELGKLASSYG 302
           QYF IKLWRVPVDK+F+ADVKA R+ INRNTI+IVGSAPGFPHGIIDPI ELG+LA SYG
Sbjct: 247 QYFKIKLWRVPVDKDFRADVKATRRHINRNTIMIVGSAPGFPHGIIDPIEELGQLALSYG 306

Query: 303 ICLHVDFCLGGFVLPFARKLGYPIPPCDFSVKGVTSISADVHKYGLAPKGT--------- 362
           IC HVD CLGGFVLPFARKLGY IPP DFSV+GVTSIS DVHKYGLAPKGT         
Sbjct: 307 ICFHVDLCLGGFVLPFARKLGYQIPPFDFSVQGVTSISVDVHKYGLAPKGTSTVLYRNHE 366

Query: 363 --------ISEWSGGLYVSPTIAGSRPGGLIAGAWAAMMSLGEEGYLQNTKEIMEVSKKI 422
                   ++EWSGGLYVSPTIAGSRPG L+AGAWAAMMSLGEEGYLQNT +IME SK++
Sbjct: 367 IRKHQFVAVTEWSGGLYVSPTIAGSRPGSLVAGAWAAMMSLGEEGYLQNTSKIMEASKRL 426

Query: 423 HKGVNEIPELFVVGKPDMTIVAFGSTVIDIFEVNDIMSSKGWHLNALQRPSSIHICVTLQ 482
            +GV EI ELFV+GKPDMTIVAFGS  +DIFEVNDIMSSKGWHLNALQRP+SIHIC+TLQ
Sbjct: 427 EEGVREIHELFVIGKPDMTIVAFGSKALDIFEVNDIMSSKGWHLNALQRPNSIHICITLQ 486

Query: 483 HVSIVDNFLQDLQESVNTVKQNPGPITGGLAPIYGAAERMPDRDMVQDLLISYMD 501
           HV +VD+FL+DL+E+V TVK NPGPITGGLAPIYGAA +MPDR MV +LL+S+MD
Sbjct: 487 HVPVVDDFLRDLREAVETVKANPGPITGGLAPIYGAAGKMPDRGMVNELLVSFMD 541

[Close]

BLAST of Carg04995 vs. ExPASy Swiss-Prot
Match: Q52RG7 (Sphingosine-1-phosphate lyase OS=Oryza sativa subsp. japonica OX=39947 GN=SPL PE=2 SV=3)

HSP 1 Score: 691.4 bits (1783), Expect = 7.5e-198
Identity = 350/533 (65.67%), Postives = 413/533 (77.49%), Query Frame = 0


Query: 8   FLVEFRASANGFLSKYEPLALVFGPLFVVLLARLLQSFLDLVYERGLKATVLSFFMATIK 67
           F +  R +AN  LS+YEPL L+  PL  +L AR L +    V +RGL+  +L+  M  IK
Sbjct: 7   FALRLRDAANHHLSRYEPLVLLAAPLLALLAARTLHAAAAAVADRGLRTVLLALAMTAIK 66

Query: 68  LIPVVKRRIDSEKQKVVDKLQSSGQSKRESWRTELPRAGLGSEVIVKMKNEKQNDVVWQG 127
           L+P V   I++EK+KVVD+LQS G S + + RTELP  GL ++VI  ++  K  DV WQG
Sbjct: 67  LLPGVSAYINAEKRKVVDQLQSGGTSTKSTLRTELPTVGLSNQVINDLETLKARDVNWQG 126

Query: 128 KCSGTV--------------------FAHTNPLHLDVFQSVVRFEAEVVAMTAALLGSKV 187
           KCSGTV                    F+HTNPLH DVF+SV + EAEVVAMTAALLG K 
Sbjct: 127 KCSGTVYIAGSESEGHFALINKAYSMFSHTNPLHQDVFKSVAQLEAEVVAMTAALLGIKE 186

Query: 188 EASGGQICGNMTSGGTESILLAVKSTRDYMKSKKGITKPEMIIPESAHSAYDKAAQYFNI 247
           ++SGGQICGNMTSGGTESILLAVK++RDYM++KKGITKPEMII ESAHSAYDKAAQYFNI
Sbjct: 187 KSSGGQICGNMTSGGTESILLAVKTSRDYMRTKKGITKPEMIIAESAHSAYDKAAQYFNI 246

Query: 248 KLWRVPVDKEFKADVKAIRKFINRNTILIVGSAPGFPHGIIDPIVELGKLASSYGICLHV 307
           K+ RVPV+KEF ADVK  ++ IN NTI++VGSAPGFPHG+IDPI ELG+LAS Y ICLHV
Sbjct: 247 KVRRVPVNKEFLADVKGFKRCINGNTIMMVGSAPGFPHGLIDPIEELGELASRYDICLHV 306

Query: 308 DFCLGGFVLPFARKLGYPIPPCDFSVKGVTSISADVHKYGLAPKGT-------------- 367
           D CLGGFVLPFARKLGYPIPP DF VKGVTSIS DVHKYGLAPKGT              
Sbjct: 307 DLCLGGFVLPFARKLGYPIPPFDFCVKGVTSISTDVHKYGLAPKGTSIVLYKNHEIRKHQ 366

Query: 368 ---ISEWSGGLYVSPTIAGSRPGGLIAGAWAAMMSLGEEGYLQNTKEIMEVSKKIHKGVN 427
              ++EW+GGLYVSPTIAGSRPGGLIAGAWAAM SLG  GY++NT  IMEVSKKI +G+ 
Sbjct: 367 FVAVTEWTGGLYVSPTIAGSRPGGLIAGAWAAMTSLGLNGYMENTGHIMEVSKKIQRGIE 426

Query: 428 EIPELFVVGKPDMTIVAFGSTVIDIFEVNDIMSSKGWHLNALQRPSSIHICVTLQHVSIV 487
           +IP LFV+GKPDMT+VAFGS  +DIFEVNDIMSSKGWHLNALQRP+S+HICVTLQH  I 
Sbjct: 427 DIPGLFVIGKPDMTVVAFGSDSVDIFEVNDIMSSKGWHLNALQRPNSLHICVTLQHTVIY 486

Query: 488 DNFLQDLQESVNTVKQNPGPITGGLAPIYGAAERMPDRDMVQDLLISYMDGTC 504
           + FL+DL++SV+TVK NPGPI+GG APIYGAA +MPDR MV++LL+ +MD +C
Sbjct: 487 EEFLKDLKDSVDTVKANPGPISGGRAPIYGAAGKMPDRGMVRELLVEFMDASC 539

[Close]

BLAST of Carg04995 vs. ExPASy Swiss-Prot
Match: Q8CHN6 (Sphingosine-1-phosphate lyase 1 OS=Rattus norvegicus OX=10116 GN=Sgpl1 PE=2 SV=1)

HSP 1 Score: 384.8 bits (987), Expect = 1.5e-105
Identity = 216/538 (40.15%), Postives = 318/538 (59.11%), Query Frame = 0


Query: 5   VKSFLVEFRASANGFLSKYEPLALVFGPLFVVLLARLLQSFLDLVYE-RGLKATVLSFFM 64
           ++++  + +   NG+ +KYEP  L+ G    VL   L+    +L+++   L +   +   
Sbjct: 19  LEAYSTKAKNYVNGYCTKYEPWQLIAGS---VLCTLLVVWVYELIFQPESLWSRFKNKLF 78

Query: 65  ATIKLIPVVKRRIDSEKQKVVDKLQSSGQSKR--ESWRTELPRAGLG-SEVIVKMKNEKQ 124
             I+ +P + R+I  +  K    L  +    +  + +   LP  GL  +EV+ ++K    
Sbjct: 79  RLIRKMPFIGRKIQQQLTKAKKDLVKNMPFLKLDKDYVKTLPAQGLSTAEVLERLKEYSS 138

Query: 125 NDVVWQ-GKCSGTV-----------------FAHTNPLHLDVFQSVVRFEAEVVAMTAAL 184
            DV WQ GK SG V                 F  +NPLH D+F  + + EAE+V MT +L
Sbjct: 139 MDVFWQEGKASGAVYSGEPKLTELLVQAYGEFTWSNPLHPDIFPGLRKLEAEIVRMTCSL 198

Query: 185 LGSKVEASGGQICGNMTSGGTESILLAVKSTRDYMKSKKGITKPEMIIPESAHSAYDKAA 244
                   G   CG +TSGGTESIL+A K+ RD +  +KGI  PE++ PESAH+A+DKAA
Sbjct: 199 FN-----GGPDSCGCVTSGGTESILMACKAYRD-LALEKGIKTPEIVAPESAHAAFDKAA 258

Query: 245 QYFNIKLWRVPVDKEFKADVKAIRKFINRNTILIVGSAPGFPHGIIDPIVELGKLASSYG 304
            YF +K+ RV   K  + DV+A+++ I+RNT ++V SAP FPHG+IDPI E+ KLA  Y 
Sbjct: 259 HYFGMKIVRVAQKKNMEVDVRAMKRAISRNTAMLVCSAPQFPHGVIDPIPEVAKLAVKYK 318

Query: 305 ICLHVDFCLGGFVLPFARKLGYPI-PPCDFSVKGVTSISADVHKYGLAPKGTI------- 364
           I  HVD CLGGF++ F  K GYP+  P DF VKGVTSISAD HKYG APKG+        
Sbjct: 319 IPFHVDACLGGFLIVFMEKAGYPLEKPFDFRVKGVTSISADTHKYGYAPKGSSVVMYSNE 378

Query: 365 ----------SEWSGGLYVSPTIAGSRPGGLIAGAWAAMMSLGEEGYLQNTKEIMEVSKK 424
                     ++W GG+Y SP+IAGSRPGG+IA  WAA+M  GE GY++ TK+I++ ++ 
Sbjct: 379 KYRKYQFFVDADWQGGIYASPSIAGSRPGGIIAACWAALMHFGENGYVEATKQIIKTARF 438

Query: 425 IHKGVNEIPELFVVGKPDMTIVAFGSTVIDIFEVNDIMSSKGWHLNALQRPSSIHICVTL 484
           +   +  I  +F++G P ++++A GS   DI+ ++++MS+KGW+ N LQ P SIH C+TL
Sbjct: 439 LKSELENIKNIFILGDPQLSVIALGSNDFDIYRLSNMMSAKGWNFNYLQFPRSIHFCITL 498

Query: 485 QHV--SIVDNFLQDLQESVNTVKQNPGPITGGLAPIYGAAERMPDRDMVQDLLISYMD 501
            H    +   FL+D++ESV  + +NP   T G+  IYG A+   DR MV ++   ++D
Sbjct: 499 VHTRKRVAIQFLKDIRESVTQIMKNPKAKTTGMGAIYGMAQATIDRKMVAEISSVFLD 547

[Close]

BLAST of Carg04995 vs. ExPASy Swiss-Prot
Match: O95470 (Sphingosine-1-phosphate lyase 1 OS=Homo sapiens OX=9606 GN=SGPL1 PE=1 SV=3)

HSP 1 Score: 379.4 bits (973), Expect = 6.3e-104
Identity = 212/525 (40.38%), Postives = 309/525 (58.86%), Query Frame = 0


Query: 17  NGFLSKYEPLALVFGPLFVVLLARLLQSFLDLVYERGLKATVLSFFMATIKLIPVVKRRI 76
           NG  +KYEP  L+   +   LL  ++  +  +     L +          + +P++ R+I
Sbjct: 31  NGHCTKYEPWQLIAWSVVWTLL--IVWGYEFVFQPESLWSRFKKKCFKLTRKMPIIGRKI 90

Query: 77  DSEKQKVVDKLQSSGQSKR--ESWRTELPRAGLGSE-VIVKMKNEKQNDVVWQ-GKCSGT 136
             +  K  D +  +    +  + +   LP  GL S  V+ K+K     D  WQ G+ SGT
Sbjct: 91  QDKLNKTKDDISKNMSFLKVDKEYVKALPSQGLSSSAVLEKLKEYSSMDAFWQEGRASGT 150

Query: 137 V-----------------FAHTNPLHLDVFQSVVRFEAEVVAMTAALLGSKVEASGGQIC 196
           V                 FA +NPLH D+F  + + EAE+V +  +L        G   C
Sbjct: 151 VYSGEEKLTELLVKAYGDFAWSNPLHPDIFPGLRKIEAEIVRIACSLFN-----GGPDSC 210

Query: 197 GNMTSGGTESILLAVKSTRDYMKSKKGITKPEMIIPESAHSAYDKAAQYFNIKLWRVPVD 256
           G +TSGGTESIL+A K+ RD +  +KGI  PE++ P+SAH+A++KAA YF +K+ RVP+ 
Sbjct: 211 GCVTSGGTESILMACKAYRD-LAFEKGIKTPEIVAPQSAHAAFNKAASYFGMKIVRVPLT 270

Query: 257 KEFKADVKAIRKFINRNTILIVGSAPGFPHGIIDPIVELGKLASSYGICLHVDFCLGGFV 316
           K  + DV+A+R+ I+RNT ++V S P FPHG+IDP+ E+ KLA  Y I LHVD CLGGF+
Sbjct: 271 KMMEVDVRAMRRAISRNTAMLVCSTPQFPHGVIDPVPEVAKLAVKYKIPLHVDACLGGFL 330

Query: 317 LPFARKLGYPIP-PCDFSVKGVTSISADVHKYGLAPKGTI-----------------SEW 376
           + F  K GYP+  P DF VKGVTSISAD HKYG APKG+                  ++W
Sbjct: 331 IVFMEKAGYPLEHPFDFRVKGVTSISADTHKYGYAPKGSSLVLYSDKKYRNYQFFVDTDW 390

Query: 377 SGGLYVSPTIAGSRPGGLIAGAWAAMMSLGEEGYLQNTKEIMEVSKKIHKGVNEIPELFV 436
            GG+Y SPTIAGSRPGG+ A  WAA+M  GE GY++ TK+I++ ++ +   +  I  +FV
Sbjct: 391 QGGIYASPTIAGSRPGGISAACWAALMHFGENGYVEATKQIIKTARFLKSELENIKGIFV 450

Query: 437 VGKPDMTIVAFGSTVIDIFEVNDIMSSKGWHLNALQRPSSIHICVTLQHV--SIVDNFLQ 496
            G P ++++A GS   DI+ ++++M++KGW+LN LQ P SIH C+TL H    +   FL+
Sbjct: 451 FGNPQLSVIALGSRDFDIYRLSNLMTAKGWNLNQLQFPPSIHFCITLLHARKRVAIQFLK 510

Query: 497 DLQESVNTVKQNPGPITGGLAPIYGAAERMPDRDMVQDLLISYMD 501
           D++ESV  + +NP   T G+  IYG A+   DR+MV +L   ++D
Sbjct: 511 DIRESVTQIMKNPKAKTTGMGAIYGMAQTTVDRNMVAELSSVFLD 547

[Close]

BLAST of Carg04995 vs. ExPASy Swiss-Prot
Match: Q8R0X7 (Sphingosine-1-phosphate lyase 1 OS=Mus musculus OX=10090 GN=Sgpl1 PE=1 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 6.3e-104
Identity = 212/538 (39.41%), Postives = 318/538 (59.11%), Query Frame = 0


Query: 5   VKSFLVEFRASANGFLSKYEPLALVFGPLFVVLLARLLQSFLDLVYE-RGLKATVLSFFM 64
           ++S+  + +   NG+ +KYEP  L+    + VL   L+    +L+++   L +       
Sbjct: 19  LESYSTKAKNYVNGYCTKYEPWQLI---AWSVLCTLLIVWVYELIFQPESLWSRFKKKLF 78

Query: 65  ATIKLIPVVKRRIDSEKQKVVDKLQSSGQSKR--ESWRTELPRAGLG-SEVIVKMKNEKQ 124
             I+ +P + R+I+ +  K    L  +    +  + +   LP  G+G +EV+ ++K    
Sbjct: 79  KLIRKMPFIGRKIEQQVSKAKKDLVKNMPFLKVDKDYVKTLPAQGMGTAEVLERLKEYSS 138

Query: 125 NDVVWQ-GKCSGTV-----------------FAHTNPLHLDVFQSVVRFEAEVVAMTAAL 184
            D  WQ GK SG V                 F  +NPLH D+F  + + EAE+V MT +L
Sbjct: 139 MDGSWQEGKASGAVYNGEPKLTELLVQAYGEFTWSNPLHPDIFPGLRKLEAEIVRMTCSL 198

Query: 185 LGSKVEASGGQICGNMTSGGTESILLAVKSTRDYMKSKKGITKPEMIIPESAHSAYDKAA 244
                   G   CG +TSGGTESIL+A K+ RD +  +KGI  PE++ PESAH+A+DKAA
Sbjct: 199 FN-----GGPDSCGCVTSGGTESILMACKAYRD-LALEKGIKTPEIVAPESAHAAFDKAA 258

Query: 245 QYFNIKLWRVPVDKEFKADVKAIRKFINRNTILIVGSAPGFPHGIIDPIVELGKLASSYG 304
            YF +K+ RV + K  + DV+A+++ I+RNT ++V S P FPHG++DP+ E+ KLA  Y 
Sbjct: 259 HYFGMKIVRVALKKNMEVDVQAMKRAISRNTAMLVCSTPQFPHGVMDPVPEVAKLAVRYK 318

Query: 305 ICLHVDFCLGGFVLPFARKLGYPI-PPCDFSVKGVTSISADVHKYGLAPKGTI------- 364
           I LHVD CLGGF++ F  K GYP+  P DF VKGVTSISAD HKYG APKG+        
Sbjct: 319 IPLHVDACLGGFLIVFMEKAGYPLEKPFDFRVKGVTSISADTHKYGYAPKGSSVVMYSNE 378

Query: 365 ----------SEWSGGLYVSPTIAGSRPGGLIAGAWAAMMSLGEEGYLQNTKEIMEVSKK 424
                     ++W GG+Y SP+IAGSRPGG+IA  WAA+M  GE GY++ TK+I++ ++ 
Sbjct: 379 KYRTYQFFVGADWQGGVYASPSIAGSRPGGIIAACWAALMHFGENGYVEATKQIIKTARF 438

Query: 425 IHKGVNEIPELFVVGKPDMTIVAFGSTVIDIFEVNDIMSSKGWHLNALQRPSSIHICVTL 484
           +   +  I  +F+ G P ++++A GS   DI+ ++++MS+KGW+ N LQ P SIH C+TL
Sbjct: 439 LKSELENIKNIFIFGDPQLSVIALGSNDFDIYRLSNMMSAKGWNFNYLQFPRSIHFCITL 498

Query: 485 QHV--SIVDNFLQDLQESVNTVKQNPGPITGGLAPIYGAAERMPDRDMVQDLLISYMD 501
            H    +   FL+D++ESV  + +NP   T G+  IYG A+   DR +V ++   ++D
Sbjct: 499 VHTRKRVAIQFLKDIRESVTQIMKNPKAKTTGMGAIYGMAQATIDRKLVAEISSVFLD 547

[Close]

BLAST of Carg04995 vs. ExPASy TrEMBL
Match: A0A6J1H7Q5 (sphingosine-1-phosphate lyase OS=Cucurbita moschata OX=3662 GN=LOC111460916 PE=3 SV=1)

HSP 1 Score: 975.3 bits (2520), Expect = 9.6e-281
Identity = 501/540 (92.78%), Postives = 503/540 (93.15%), Query Frame = 0


Query: 1   MDSAVKSFLVEFRASANGFLSKYEPLALVFGPLFVVLLARLLQSFLDLVYERGLKATVLS 60
           MDSAVKSFLVEFRASANGFLSKYEPLALVFGPLFV+LLARLLQSFLDLVYERGLKATVLS
Sbjct: 1   MDSAVKSFLVEFRASANGFLSKYEPLALVFGPLFVILLARLLQSFLDLVYERGLKATVLS 60

Query: 61  FFMATIKLIPVVKRRIDSEKQKVVDKLQSSGQSKRESWRTELPRAGLGSEVIVKMKNEKQ 120
           FFMATIKLIPVVKRRIDSEKQKVVDKLQSSGQSKRESWRTELPRAGLGSEVIVKMKNEKQ
Sbjct: 61  FFMATIKLIPVVKRRIDSEKQKVVDKLQSSGQSKRESWRTELPRAGLGSEVIVKMKNEKQ 120

Query: 121 NDVVWQGKCSGTV--------------------FAHTNPLHLDVFQSVVRFEAEVVAMTA 180
           NDVVWQGKCSGTV                    FAHTNPLHLDVFQSVVRFEAEVVAMTA
Sbjct: 121 NDVVWQGKCSGTVYIGGTESEGHFSLINEACSMFAHTNPLHLDVFQSVVRFEAEVVAMTA 180

Query: 181 ALLGSKVEASGGQICGNMTSGGTESILLAVKSTRDYMKSKKGITKPEMIIPESAHSAYDK 240
           ALLGSKVEASGGQICGNMTSGGTESILLAVKSTRDYMKSKKGITKPEMIIPESAHSAYDK
Sbjct: 181 ALLGSKVEASGGQICGNMTSGGTESILLAVKSTRDYMKSKKGITKPEMIIPESAHSAYDK 240

Query: 241 AAQYFNIKLWRVPVDKEFKADVKAIRKFINRNTILIVGSAPGFPHGIIDPIVELGKLASS 300
           AAQYFNIKLWRVPVDKEFKADVKAIRKFINRNTILIVGSAPGFPHGIIDPIVELGKLASS
Sbjct: 241 AAQYFNIKLWRVPVDKEFKADVKAIRKFINRNTILIVGSAPGFPHGIIDPIVELGKLASS 300

Query: 301 YGICLHVDFCLGGFVLPFARKLGYPIPPCDFSVKGVTSISADVHKYGLAPKGT------- 360
           YGICLHVDFCLGGFVLPFARKLGYPIPPCDFSVKGVTSISADVHKYGLAPKGT       
Sbjct: 301 YGICLHVDFCLGGFVLPFARKLGYPIPPCDFSVKGVTSISADVHKYGLAPKGTSIVLYRN 360

Query: 361 ----------ISEWSGGLYVSPTIAGSRPGGLIAGAWAAMMSLGEEGYLQNTKEIMEVSK 420
                     +SEWSGGLYVSPTIAGSRPGGLIAGAWAAMMSLGEEGYLQNTKEIMEVSK
Sbjct: 361 HEIRKHQFVAVSEWSGGLYVSPTIAGSRPGGLIAGAWAAMMSLGEEGYLQNTKEIMEVSK 420

Query: 421 KIHKGVNEIPELFVVGKPDMTIVAFGSTVIDIFEVNDIMSSKGWHLNALQRPSSIHICVT 480
           KIHKGVNEIPELFVVGKPDMTIVAFGSTVIDIFEVNDIMSSKGWHLNALQRPSSIHICVT
Sbjct: 421 KIHKGVNEIPELFVVGKPDMTIVAFGSTVIDIFEVNDIMSSKGWHLNALQRPSSIHICVT 480

Query: 481 LQHVSIVDNFLQDLQESVNTVKQNPGPITGGLAPIYGAAERMPDRDMVQDLLISYMDGTC 504
           LQHVSIVDNFLQDLQESVNTVKQNPGPITGGLAPIYGAAERMPDRDMVQDLLISYMDGTC
Sbjct: 481 LQHVSIVDNFLQDLQESVNTVKQNPGPITGGLAPIYGAAERMPDRDMVQDLLISYMDGTC 540

[Close]

BLAST of Carg04995 vs. ExPASy TrEMBL
Match: A0A6J1KY89 (sphingosine-1-phosphate lyase OS=Cucurbita maxima OX=3661 GN=LOC111498204 PE=3 SV=1)

HSP 1 Score: 959.9 bits (2480), Expect = 4.2e-276
Identity = 495/540 (91.67%), Postives = 498/540 (92.22%), Query Frame = 0


Query: 1   MDSAVKSFLVEFRASANGFLSKYEPLALVFGPLFVVLLARLLQSFLDLVYERGLKATVLS 60
           MDSAVKSFLVEFRASANGFLSKYEPLALVFGPLFV LLARLLQSFLDLVYERGLKAT LS
Sbjct: 1   MDSAVKSFLVEFRASANGFLSKYEPLALVFGPLFVTLLARLLQSFLDLVYERGLKATFLS 60

Query: 61  FFMATIKLIPVVKRRIDSEKQKVVDKLQSSGQSKRESWRTELPRAGLGSEVIVKMKNEKQ 120
           FFMATIKLIPVVKRRIDSEKQKVVDKLQSSGQSKRESWRTELPRAGLGSEVIVKMKNEKQ
Sbjct: 61  FFMATIKLIPVVKRRIDSEKQKVVDKLQSSGQSKRESWRTELPRAGLGSEVIVKMKNEKQ 120

Query: 121 NDVVWQGKCSGTV--------------------FAHTNPLHLDVFQSVVRFEAEVVAMTA 180
           NDVVWQGKCSGTV                    FAHTNPLHLDVFQSVVRFEAEVVAMTA
Sbjct: 121 NDVVWQGKCSGTVYIGGTESEGHFSLINEACSMFAHTNPLHLDVFQSVVRFEAEVVAMTA 180

Query: 181 ALLGSKVEASGGQICGNMTSGGTESILLAVKSTRDYMKSKKGITKPEMIIPESAHSAYDK 240
           ALLGSKVEASGGQICGNMTSGGTESILLAVKS+RDYMKSKKGITKPEMIIPESAHSAYDK
Sbjct: 181 ALLGSKVEASGGQICGNMTSGGTESILLAVKSSRDYMKSKKGITKPEMIIPESAHSAYDK 240

Query: 241 AAQYFNIKLWRVPVDKEFKADVKAIRKFINRNTILIVGSAPGFPHGIIDPIVELGKLASS 300
           AAQYFNIKLWRVPVD EFKADVKAIRKFINRNTILIVGSAPGFPHGIIDPIVELGKLASS
Sbjct: 241 AAQYFNIKLWRVPVDTEFKADVKAIRKFINRNTILIVGSAPGFPHGIIDPIVELGKLASS 300

Query: 301 YGICLHVDFCLGGFVLPFARKLGYPIPPCDFSVKGVTSISADVHKYGLAPKGT------- 360
            GICLHVDFCLGGFVLPFA KLGYPIPPCDFSVKGVTSISADVHKYGLAPKGT       
Sbjct: 301 SGICLHVDFCLGGFVLPFACKLGYPIPPCDFSVKGVTSISADVHKYGLAPKGTSVVLYRN 360

Query: 361 ----------ISEWSGGLYVSPTIAGSRPGGLIAGAWAAMMSLGEEGYLQNTKEIMEVSK 420
                     +SEWSGGLYVSPTIAGSRPGGLIAGAWAAMMSLGEEGYLQNTKEIMEVSK
Sbjct: 361 HEIRKHQFVAVSEWSGGLYVSPTIAGSRPGGLIAGAWAAMMSLGEEGYLQNTKEIMEVSK 420

Query: 421 KIHKGVNEIPELFVVGKPDMTIVAFGSTVIDIFEVNDIMSSKGWHLNALQRPSSIHICVT 480
           KIHKGVNEIPELFVVGKPDMTIVAFGSTVIDIFEVNDIMSSKGWHLNALQRPSSIHICVT
Sbjct: 421 KIHKGVNEIPELFVVGKPDMTIVAFGSTVIDIFEVNDIMSSKGWHLNALQRPSSIHICVT 480

Query: 481 LQHVSIVDNFLQDLQESVNTVKQNPGPITGGLAPIYGAAERMPDRDMVQDLLISYMDGTC 504
           LQHVSIVD+FLQDLQESVNTVKQNPGPITGGLAPIYGAAERMPDRDMVQDLLISYMDGTC
Sbjct: 481 LQHVSIVDDFLQDLQESVNTVKQNPGPITGGLAPIYGAAERMPDRDMVQDLLISYMDGTC 540

[Close]

BLAST of Carg04995 vs. ExPASy TrEMBL
Match: A0A0A0KC99 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G094720 PE=3 SV=1)

HSP 1 Score: 912.1 bits (2356), Expect = 9.9e-262
Identity = 464/540 (85.93%), Postives = 485/540 (89.81%), Query Frame = 0


Query: 1   MDSAVKSFLVEFRASANGFLSKYEPLALVFGPLFVVLLARLLQSFLDLVYERGLKATVLS 60
           MDS++KS LVEFRASAN FLSKYEPLALVFGPL V+LLAR+LQSFLDLVYE GLKAT LS
Sbjct: 1   MDSSLKSVLVEFRASANAFLSKYEPLALVFGPLLVLLLARVLQSFLDLVYEAGLKATCLS 60

Query: 61  FFMATIKLIPVVKRRIDSEKQKVVDKLQSSGQSKRESWRTELPRAGLGSEVIVKMKNEKQ 120
           FFMATIKLIP VKRRIDSEKQKVV+KLQSSG SKRESWRTELP AGLG+EVI KMK+EKQ
Sbjct: 61  FFMATIKLIPGVKRRIDSEKQKVVEKLQSSGNSKRESWRTELPMAGLGTEVIEKMKSEKQ 120

Query: 121 NDVVWQGKCSGTV--------------------FAHTNPLHLDVFQSVVRFEAEVVAMTA 180
           NDVVWQGKCSGTV                    FAHTNPLHLDVFQSVVRFEAEVVAMTA
Sbjct: 121 NDVVWQGKCSGTVYIGGSESEGHFSIINEACSMFAHTNPLHLDVFQSVVRFEAEVVAMTA 180

Query: 181 ALLGSKVEASGGQICGNMTSGGTESILLAVKSTRDYMKSKKGITKPEMIIPESAHSAYDK 240
           A+LGSKVEASGGQICGNMTSGGTESILLAVKSTRDYMKSK+GI KPEMIIPESAHSAYDK
Sbjct: 181 AILGSKVEASGGQICGNMTSGGTESILLAVKSTRDYMKSKRGIKKPEMIIPESAHSAYDK 240

Query: 241 AAQYFNIKLWRVPVDKEFKADVKAIRKFINRNTILIVGSAPGFPHGIIDPIVELGKLASS 300
           AA YFNIKLWRVPVDKEFKADVKAIRKFINRNTILIVGSAPGFPHGIIDPIVELG+LASS
Sbjct: 241 AAHYFNIKLWRVPVDKEFKADVKAIRKFINRNTILIVGSAPGFPHGIIDPIVELGELASS 300

Query: 301 YGICLHVDFCLGGFVLPFARKLGYPIPPCDFSVKGVTSISADVHKYGLAPKGT------- 360
            GICLHVDFCLGGFVLPFARKLGYPIPPCDFSVKGVTSIS DVHKYGLAPKGT       
Sbjct: 301 SGICLHVDFCLGGFVLPFARKLGYPIPPCDFSVKGVTSISVDVHKYGLAPKGTSVVLYRN 360

Query: 361 ----------ISEWSGGLYVSPTIAGSRPGGLIAGAWAAMMSLGEEGYLQNTKEIMEVSK 420
                     +SEWSGGLYVSPTIAGSRPGGLIAGAWAAMMSLGEEGYLQNTKEIMEVSK
Sbjct: 361 HEIRKHQFVAVSEWSGGLYVSPTIAGSRPGGLIAGAWAAMMSLGEEGYLQNTKEIMEVSK 420

Query: 421 KIHKGVNEIPELFVVGKPDMTIVAFGSTVIDIFEVNDIMSSKGWHLNALQRPSSIHICVT 480
           +IH+G+NEIPELFV+GKPDMTI+AFGST +DIFEVNDIMSSKGWHLNALQ+PSSIHICVT
Sbjct: 421 RIHRGINEIPELFVIGKPDMTIIAFGSTALDIFEVNDIMSSKGWHLNALQKPSSIHICVT 480

Query: 481 LQHVSIVDNFLQDLQESVNTVKQNPGPITGGLAPIYGAAERMPDRDMVQDLLISYMDGTC 504
           LQHVSIVD+FLQDLQESV  VK+NPGPI GGLAPIYGAAERMPDR MVQ+LLISYMDGTC
Sbjct: 481 LQHVSIVDDFLQDLQESVTIVKKNPGPINGGLAPIYGAAERMPDRSMVQELLISYMDGTC 540

[Close]

BLAST of Carg04995 vs. ExPASy TrEMBL
Match: A0A1S3CBM0 (sphingosine-1-phosphate lyase OS=Cucumis melo OX=3656 GN=LOC103498855 PE=3 SV=1)

HSP 1 Score: 910.2 bits (2351), Expect = 3.8e-261
Identity = 464/540 (85.93%), Postives = 482/540 (89.26%), Query Frame = 0


Query: 1   MDSAVKSFLVEFRASANGFLSKYEPLALVFGPLFVVLLARLLQSFLDLVYERGLKATVLS 60
           MDS++ S LVE+RASAN FLSKYEPLALVFGPL V+LLARLLQSFLDLVYE GLKAT LS
Sbjct: 1   MDSSLNSVLVEYRASANAFLSKYEPLALVFGPLLVLLLARLLQSFLDLVYEAGLKATFLS 60

Query: 61  FFMATIKLIPVVKRRIDSEKQKVVDKLQSSGQSKRESWRTELPRAGLGSEVIVKMKNEKQ 120
           FFMATIKLIP VKRRIDSEKQKVV+KLQS G SKRESWRTELP AGLG+EVI KMKNEKQ
Sbjct: 61  FFMATIKLIPGVKRRIDSEKQKVVEKLQSGGNSKRESWRTELPMAGLGTEVIEKMKNEKQ 120

Query: 121 NDVVWQGKCSGTV--------------------FAHTNPLHLDVFQSVVRFEAEVVAMTA 180
            DVVWQGKCSGTV                    FAHTNPLHLDVFQSVVRFEAEVVAMTA
Sbjct: 121 KDVVWQGKCSGTVYIGGSESEGHFSLINEACSMFAHTNPLHLDVFQSVVRFEAEVVAMTA 180

Query: 181 ALLGSKVEASGGQICGNMTSGGTESILLAVKSTRDYMKSKKGITKPEMIIPESAHSAYDK 240
           ALLGSKVEASGGQICGNMTSGGTESILLAVKSTRDYMKSKKGI KPEMIIPESAHSAYDK
Sbjct: 181 ALLGSKVEASGGQICGNMTSGGTESILLAVKSTRDYMKSKKGIKKPEMIIPESAHSAYDK 240

Query: 241 AAQYFNIKLWRVPVDKEFKADVKAIRKFINRNTILIVGSAPGFPHGIIDPIVELGKLASS 300
           AA YFNIKLWRVPV+KEFKADVKAIRKFINRNTILIVGSAPGFPHGIIDPIVELG+LASS
Sbjct: 241 AAHYFNIKLWRVPVNKEFKADVKAIRKFINRNTILIVGSAPGFPHGIIDPIVELGELASS 300

Query: 301 YGICLHVDFCLGGFVLPFARKLGYPIPPCDFSVKGVTSISADVHKYGLAPKGT------- 360
            GICLHVDFCLGGFVLPFARKLGYPIPPCDFSVKGVTSIS DVHKYGLAPKGT       
Sbjct: 301 SGICLHVDFCLGGFVLPFARKLGYPIPPCDFSVKGVTSISVDVHKYGLAPKGTSVVLYRN 360

Query: 361 ----------ISEWSGGLYVSPTIAGSRPGGLIAGAWAAMMSLGEEGYLQNTKEIMEVSK 420
                     +SEWSGGLYVSPTIAGSRPGGLIAGAWAAMMSLGEEGYLQNTKEIMEVSK
Sbjct: 361 HEIRKHQFVAVSEWSGGLYVSPTIAGSRPGGLIAGAWAAMMSLGEEGYLQNTKEIMEVSK 420

Query: 421 KIHKGVNEIPELFVVGKPDMTIVAFGSTVIDIFEVNDIMSSKGWHLNALQRPSSIHICVT 480
           +IH+G+NEIPELFV+GKPDMTI+AFGST +DIFEVNDIMSSKGWHLNALQ+PSSIHICVT
Sbjct: 421 RIHRGINEIPELFVIGKPDMTIIAFGSTALDIFEVNDIMSSKGWHLNALQKPSSIHICVT 480

Query: 481 LQHVSIVDNFLQDLQESVNTVKQNPGPITGGLAPIYGAAERMPDRDMVQDLLISYMDGTC 504
           LQHVSIVDNFLQDLQESV  VK+NPGPI GGLAPIYGAAERMPDR MVQ+LLISYMDGTC
Sbjct: 481 LQHVSIVDNFLQDLQESVTIVKKNPGPINGGLAPIYGAAERMPDRSMVQELLISYMDGTC 540

[Close]

BLAST of Carg04995 vs. ExPASy TrEMBL
Match: A0A5A7T981 (Sphingosine-1-phosphate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G002270 PE=3 SV=1)

HSP 1 Score: 902.5 bits (2331), Expect = 7.9e-259
Identity = 461/537 (85.85%), Postives = 479/537 (89.20%), Query Frame = 0


Query: 1   MDSAVKSFLVEFRASANGFLSKYEPLALVFGPLFVVLLARLLQSFLDLVYERGLKATVLS 60
           MDS++ S LVE+RASAN FLSKYEPLALVFGPL V+LLARLLQSFLDLVYE GLKAT LS
Sbjct: 1   MDSSLNSVLVEYRASANAFLSKYEPLALVFGPLLVLLLARLLQSFLDLVYEAGLKATFLS 60

Query: 61  FFMATIKLIPVVKRRIDSEKQKVVDKLQSSGQSKRESWRTELPRAGLGSEVIVKMKNEKQ 120
           FFMATIKLIP VKRRIDSEKQKVV+KLQS G SKRESWRTELP AGLG+EVI KMKNEKQ
Sbjct: 61  FFMATIKLIPGVKRRIDSEKQKVVEKLQSGGNSKRESWRTELPMAGLGTEVIEKMKNEKQ 120

Query: 121 NDVVWQGKCSGTV--------------------FAHTNPLHLDVFQSVVRFEAEVVAMTA 180
            DVVWQGKCSGTV                    FAHTNPLHLDVFQSVVRFEAEVVAMTA
Sbjct: 121 KDVVWQGKCSGTVYIGGSESEGHFSLINEACSMFAHTNPLHLDVFQSVVRFEAEVVAMTA 180

Query: 181 ALLGSKVEASGGQICGNMTSGGTESILLAVKSTRDYMKSKKGITKPEMIIPESAHSAYDK 240
           ALLGSKVEASGGQICGNMTSGGTESILLAVKSTRDYMKSKKGI KPEMIIPESAHSAYDK
Sbjct: 181 ALLGSKVEASGGQICGNMTSGGTESILLAVKSTRDYMKSKKGIKKPEMIIPESAHSAYDK 240

Query: 241 AAQYFNIKLWRVPVDKEFKADVKAIRKFINRNTILIVGSAPGFPHGIIDPIVELGKLASS 300
           AA YFNIKLWRVPV+KEFKADVKAIRKFINRNTILIVGSAPGFPHGIIDPIVELG+LASS
Sbjct: 241 AAHYFNIKLWRVPVNKEFKADVKAIRKFINRNTILIVGSAPGFPHGIIDPIVELGELASS 300

Query: 301 YGICLHVDFCLGGFVLPFARKLGYPIPPCDFSVKGVTSISADVHKYGLAPKGT------- 360
            GICLHVDFCLGGFVLPFARKLGYPIPPCDFSVKGVTSIS DVHKYGLAPKGT       
Sbjct: 301 SGICLHVDFCLGGFVLPFARKLGYPIPPCDFSVKGVTSISVDVHKYGLAPKGTSVVLYRN 360

Query: 361 ----------ISEWSGGLYVSPTIAGSRPGGLIAGAWAAMMSLGEEGYLQNTKEIMEVSK 420
                     +SEWSGGLYVSPTIAGSRPGGLIAGAWAAMMSLGEEGYLQNTKEIMEVSK
Sbjct: 361 HEIRKHQFVAVSEWSGGLYVSPTIAGSRPGGLIAGAWAAMMSLGEEGYLQNTKEIMEVSK 420

Query: 421 KIHKGVNEIPELFVVGKPDMTIVAFGSTVIDIFEVNDIMSSKGWHLNALQRPSSIHICVT 480
           +IH+G+NEIPELFV+GKPDMTI+AFGST +DIFEVNDIMSSKGWHLNALQ+PSSIHICVT
Sbjct: 421 RIHRGINEIPELFVIGKPDMTIIAFGSTALDIFEVNDIMSSKGWHLNALQKPSSIHICVT 480

Query: 481 LQHVSIVDNFLQDLQESVNTVKQNPGPITGGLAPIYGAAERMPDRDMVQDLLISYMD 501
           LQHVSIVDNFLQDLQESV  VK+NPGPI GGLAPIYGAAERMPDR MVQ+LLISYMD
Sbjct: 481 LQHVSIVDNFLQDLQESVTVVKKNPGPINGGLAPIYGAAERMPDRSMVQELLISYMD 537

[Close]

BLAST of Carg04995 vs. TAIR 10
Match: AT1G27980.1 (dihydrosphingosine phosphate lyase )

HSP 1 Score: 747.3 bits (1928), Expect = 8.1e-216
Identity = 375/535 (70.09%), Postives = 434/535 (81.12%), Query Frame = 0


Query: 3   SAVKSFLVEFRASANGFLSKYEPLALVFGPLFVVLLARLLQSFLDLVYERGLKATVLSFF 62
           S++KS L++ R S N  LS++EPL L+  PL  + LA+++ S   +V+E+GLKA ++ F 
Sbjct: 7   SSMKSMLIQARGSLNSRLSEFEPLVLLLVPLVSLFLAQIIGSVFGVVHEKGLKACLIGFI 66

Query: 63  MATIKLIPVVKRRIDSEKQKVVDKLQSSGQSKRESWRTELPRAGLGSEVIVKMKNEKQND 122
           M  +K+IP V+  ID+EKQKVVD+LQS   SK+++    LP  GLG EV+ KM+NEK+ND
Sbjct: 67  MGLLKMIPGVQNYIDAEKQKVVDQLQSGSSSKKKNKTEVLPVKGLGVEVLEKMENEKRND 126

Query: 123 VVWQGKCSGTV--------------------FAHTNPLHLDVFQSVVRFEAEVVAMTAAL 182
            +WQGKCSGTV                    FAHTNPLH+DVFQSVVRFE+EVVAMTAAL
Sbjct: 127 AIWQGKCSGTVYIGGAESEGHFSLINQACSMFAHTNPLHIDVFQSVVRFESEVVAMTAAL 186

Query: 183 LGSKVEASGGQICGNMTSGGTESILLAVKSTRDYMKSKKGITKPEMIIPESAHSAYDKAA 242
           LGSK  ASGGQICGNMTSGGTESI+LAVKS+RDYMK KKGIT+PEMIIPES HSAYDKAA
Sbjct: 187 LGSKETASGGQICGNMTSGGTESIVLAVKSSRDYMKYKKGITRPEMIIPESGHSAYDKAA 246

Query: 243 QYFNIKLWRVPVDKEFKADVKAIRKFINRNTILIVGSAPGFPHGIIDPIVELGKLASSYG 302
           QYF IKLWRVPVDK+F+ADVKA R+ INRNTI+IVGSAPGFPHGIIDPI ELG+LA SYG
Sbjct: 247 QYFKIKLWRVPVDKDFRADVKATRRHINRNTIMIVGSAPGFPHGIIDPIEELGQLALSYG 306

Query: 303 ICLHVDFCLGGFVLPFARKLGYPIPPCDFSVKGVTSISADVHKYGLAPKGT--------- 362
           IC HVD CLGGFVLPFARKLGY IPP DFSV+GVTSIS DVHKYGLAPKGT         
Sbjct: 307 ICFHVDLCLGGFVLPFARKLGYQIPPFDFSVQGVTSISVDVHKYGLAPKGTSTVLYRNHE 366

Query: 363 --------ISEWSGGLYVSPTIAGSRPGGLIAGAWAAMMSLGEEGYLQNTKEIMEVSKKI 422
                   ++EWSGGLYVSPTIAGSRPG L+AGAWAAMMSLGEEGYLQNT +IME SK++
Sbjct: 367 IRKHQFVAVTEWSGGLYVSPTIAGSRPGSLVAGAWAAMMSLGEEGYLQNTSKIMEASKRL 426

Query: 423 HKGVNEIPELFVVGKPDMTIVAFGSTVIDIFEVNDIMSSKGWHLNALQRPSSIHICVTLQ 482
            +GV EI ELFV+GKPDMTIVAFGS  +DIFEVNDIMSSKGWHLNALQRP+SIHIC+TLQ
Sbjct: 427 EEGVREIHELFVIGKPDMTIVAFGSKALDIFEVNDIMSSKGWHLNALQRPNSIHICITLQ 486

Query: 483 HVSIVDNFLQDLQESVNTVKQNPGPITGGLAPIYGAAERMPDRDMVQDLLISYMD 501
           HV +VD+FL+DL+E+V TVK NPGPITGGLAPIYGAA +MPDR MV +LL+S+MD
Sbjct: 487 HVPVVDDFLRDLREAVETVKANPGPITGGLAPIYGAAGKMPDRGMVNELLVSFMD 541

[Close]

BLAST of Carg04995 vs. TAIR 10
Match: AT4G28680.3 (L-tyrosine decarboxylase )

HSP 1 Score: 50.1 bits (118), Expect = 6.2e-06
Identity = 45/173 (26.01%), Postives = 79/173 (45.66%), Query Frame = 0


Query: 137 TNPLHLDVFQSVVRFEAEVVAMTAALLGSKVEASGGQICGNMTSGGTESILLAVKSTRDY 196
           T+P   ++   V+ + A+++ +    L +     GG I G     G E++L+ V + RD 
Sbjct: 170 TSPAATELEIIVLDWLAKLLQLPDHFLSTGKGNGGGVIQGT----GCEAVLVVVLAARDR 229

Query: 197 MKSKKGIT-KPEMII--PESAHSAYDKAA-----QYFNIKLWRVPVDKEFKADVKAIRKF 256
           +  K G T  P++++   +  HS++ KA         NI+L +      +    +++ + 
Sbjct: 230 ILKKVGKTLLPQLVVYGSDQTHSSFRKACLIGGIHEENIRLLKTDSSTNYGMPPESLEEA 289

Query: 257 INRNTILIVGSAPGF--------PHGIIDPIVELGKLASSYGICLHVDFCLGG 294
           I+ +  L  G  P F            +DP+V LG +A  YGI LHVD    G
Sbjct: 290 ISHD--LAKGFIPFFICATVGTTSSAAVDPLVPLGNIAKKYGIWLHVDAAYAG 336

[Close]

BLAST of Carg04995 vs. TAIR 10
Match: AT4G28680.4 (L-tyrosine decarboxylase )

HSP 1 Score: 50.1 bits (118), Expect = 6.2e-06
Identity = 45/173 (26.01%), Postives = 79/173 (45.66%), Query Frame = 0


Query: 137 TNPLHLDVFQSVVRFEAEVVAMTAALLGSKVEASGGQICGNMTSGGTESILLAVKSTRDY 196
           T+P   ++   V+ + A+++ +    L +     GG I G     G E++L+ V + RD 
Sbjct: 161 TSPAATELEIIVLDWLAKLLQLPDHFLSTGKGNGGGVIQGT----GCEAVLVVVLAARDR 220

Query: 197 MKSKKGIT-KPEMII--PESAHSAYDKAA-----QYFNIKLWRVPVDKEFKADVKAIRKF 256
           +  K G T  P++++   +  HS++ KA         NI+L +      +    +++ + 
Sbjct: 221 ILKKVGKTLLPQLVVYGSDQTHSSFRKACLIGGIHEENIRLLKTDSSTNYGMPPESLEEA 280

Query: 257 INRNTILIVGSAPGF--------PHGIIDPIVELGKLASSYGICLHVDFCLGG 294
           I+ +  L  G  P F            +DP+V LG +A  YGI LHVD    G
Sbjct: 281 ISHD--LAKGFIPFFICATVGTTSSAAVDPLVPLGNIAKKYGIWLHVDAAYAG 327

The following BLAST results are available for this feature:

BLAST of Carg04995 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7025365.1	1.7e-287	100.00	Sphingosine-1-phosphate lyase, partial [Cucurbita argyrosperma subsp. argyrosper...	[more]
KAG6592955.1	1.5e-280	92.96	Sphingosine-1-phosphate lyase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022960048.1	2.0e-280	92.78	sphingosine-1-phosphate lyase [Cucurbita moschata]	[more]
XP_023514914.1	1.3e-279	92.41	sphingosine-1-phosphate lyase [Cucurbita pepo subsp. pepo]	[more]
XP_023005114.1	8.6e-276	91.67	sphingosine-1-phosphate lyase [Cucurbita maxima]	[more]

BLAST of Carg04995 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C509	1.1e-214	70.09	Sphingosine-1-phosphate lyase OS=Arabidopsis thaliana OX=3702 GN=DPL1 PE=1 SV=1	[more]
Q52RG7	7.5e-198	65.67	Sphingosine-1-phosphate lyase OS=Oryza sativa subsp. japonica OX=39947 GN=SPL PE...	[more]
Q8CHN6	1.5e-105	40.15	Sphingosine-1-phosphate lyase 1 OS=Rattus norvegicus OX=10116 GN=Sgpl1 PE=2 SV=1	[more]
O95470	6.3e-104	40.38	Sphingosine-1-phosphate lyase 1 OS=Homo sapiens OX=9606 GN=SGPL1 PE=1 SV=3	[more]
Q8R0X7	6.3e-104	39.41	Sphingosine-1-phosphate lyase 1 OS=Mus musculus OX=10090 GN=Sgpl1 PE=1 SV=1	[more]

BLAST of Carg04995 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H7Q5	9.6e-281	92.78	sphingosine-1-phosphate lyase OS=Cucurbita moschata OX=3662 GN=LOC111460916 PE=3...	[more]
A0A6J1KY89	4.2e-276	91.67	sphingosine-1-phosphate lyase OS=Cucurbita maxima OX=3661 GN=LOC111498204 PE=3 S...	[more]
A0A0A0KC99	9.9e-262	85.93	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G094720 PE=3 SV=1	[more]
A0A1S3CBM0	3.8e-261	85.93	sphingosine-1-phosphate lyase OS=Cucumis melo OX=3656 GN=LOC103498855 PE=3 SV=1	[more]
A0A5A7T981	7.9e-259	85.85	Sphingosine-1-phosphate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]

BLAST of Carg04995 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G27980.1	8.1e-216	70.09	dihydrosphingosine phosphate lyase	[more]
AT4G28680.3	6.2e-06	26.01	L-tyrosine decarboxylase	[more]
AT4G28680.4	6.2e-06	26.01	L-tyrosine decarboxylase	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002129	Pyridoxal phosphate-dependent decarboxylase	PFAM	PF00282	Pyridoxal_deC	coord: 148..330 e-value: 1.4E-13 score: 50.4
IPR015421	Pyridoxal phosphate-dependent transferase, major domain	GENE3D	3.40.640.10		coord: 117..367 e-value: 2.7E-152 score: 509.1
IPR015422	Pyridoxal phosphate-dependent transferase, small domain	GENE3D	3.90.1150.10	Aspartate Aminotransferase, domain 1	coord: 80..466 e-value: 2.7E-152 score: 509.1
None	No IPR available	GENE3D	6.10.140.2150		coord: 79..500 e-value: 2.7E-152 score: 509.1
None	No IPR available	PIRSR	PIRSR602129-50	PIRSR602129-50	coord: 74..333 e-value: 1.0E-78 score: 262.6
None	No IPR available	PANTHER	PTHR42735:SF6	SPHINGOSINE-1-PHOSPHATE LYASE 1	coord: 20..503
None	No IPR available	PANTHER	PTHR42735	FAMILY NOT NAMED	coord: 20..503
IPR015424	Pyridoxal phosphate-dependent transferase	SUPERFAMILY	53383	PLP-dependent transferases	coord: 82..466

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg04995-RA	Carg04995-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019752	carboxylic acid metabolic process
biological_process	GO:0030149	sphingolipid catabolic process
cellular_component	GO:0005783	endoplasmic reticulum
molecular_function	GO:0030170	pyridoxal phosphate binding
molecular_function	GO:0008117	sphinganine-1-phosphate aldolase activity
molecular_function	GO:0016830	carbon-carbon lyase activity
molecular_function	GO:0003824	catalytic activity