Carg04976 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg04976
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionautophagy-related protein 3-like
LocationCarg_Chr08: 797236 .. 804518 (-)
RNA-Seq ExpressionCarg04976
SyntenyCarg04976
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTTTGTCGCAGAAGCTTCACGAGGCCTTCAAAGGAACTGTCGAGAGGATCACAGGGCCTCGAACCGTTTCCGCCTTCAAAGAGAAAGGCGTTCTCAGTGTCTCTGAGTTCATCGTCGCCGGTGATAATCTCGTCTCCAAGTGTCCCACCTGGTCTTGGTATTTTCATGTGAATTCTCTCCATTTTCCTGATCGAAGTTGTTTTATATATTTGTTGAATTTGTTCTTTTGCGATATTCGAAATTGATAGACGTGTTCTTCAACTGATGAATTGAACGGATGTCTCTATTAGAAGATTCCGTGCATTCATCAATTATATCAATTCTCTATCCCGAATCCTGTAATTTTTGTCAATTCTGTTTTCCAAGATACTGAGGTCGTTTATGGCTGTTCGAACGAAACCAAGGTTAATTGGTCTAGGAGGTGAGGTCTGTGCCGGTGTCAAAGCCTTTTATTGTAAACTTGGATGAAGGAGTTTGGTTATCTCCGTTTTAGTGAATTGGAATGGTTGGGAAAAAAATATTTAATGATTTGATTAAAATGCCTTCTGAATCAAAGGTTCTTGAGAAAGCTAAAGAGGAGAGTGGTACATATTGTGTGTAGAAAGGACATTAATAATAGTGATATCCCATTAGTGTTTCTTATTTTTCCTGAAACCATGACTACGACCTTCGTTTGACTTAAGTTTGTTATTTGTTTTTGAATTATAAAATTTAGTGGAACAGAATTAGAGTGGTATCCATTGCTTCTGTATGCGTACTTAGTTTTAGGTTCTTCATAGTTCGGAAGGGCAGTAATAACCCAAATGTCGACTATCCAGTGTATGTTATACACCGTCCCGTCCCTTTTTCAGGCGAAAGACAATGTGGATCTTTCCACAATTTAAAAAACTGACATTATTGGTGGTTCACTTTTACAGTTGCACTCTTAATTTTCTCACCTCCTGTATTTGGAATTAAAATTTGGAAATGTTTTGAATTTATCTAATACATGTTAAAATATGTTTTCCTTTTTCATGATTTTAGGGAGTCAGGTGATCTGAATAAGAGGAAATCATACTTGCCCCCAGATAAGCAATTCTTAATTACCAGAAATGGTATAACTGCATAATCATTAACTTTTTTTCTTTTCTTTTTTTTGTAATTCGAGGGGTTACAGTCAGAACCGTAATGTTTTGTAATTTTCTGTCCATACCTTGTAGTTTATGTGCTTTTGACATTTACACTTCTCCTTCCCATAATCTTCTATTAATTTATGGTCATTTATCTGAAATATTGTTATAAACAAAAAGAATTTTGCATGTTATCAGGCCATTTTTCTAAACCATACTCTTTCTACTCATGCCTTAAAATTATGTCGCTCTCCACAATGAGACTTACACTTGGCAGGGAACCTAATCAATAGAAACAACTCACGTTTCAATACCAAACATGAATTACTATTTTCTGCAACATATAGTGCACCTTTGAGACACGGAGGTCAAGTTTGTATGTATATCTTTTCTAGAAATTAGTATTTAACCTGTAGCTAATTGCCAAATTAATTCTTTGGTAACTGTTTCAGCACCGGTAAGGTCAATTATATACTTTTTGCAACTAATGGTTTGGCGTTATGTCAAATATTGAATGAATTCTTTGGCATATCTAGACTTCAACTTTATTGAGCCATTATCAAAAGTAAGGATGATCGTTCACTTTCTTTCCAGTTCCTTGCCTTCGCCGGGCTGCCTATGTAGAAGAGGAGTATGAAGCTGCAGGTGGTGAAGTTTTACTTGATGAGGAAGATGGCGATGGCTGGCTGGCAACTCATGGCAAACCGAAGGGTACCTTTATCTTTCAGTGTAAAGCTAGGGATTTACATTTGTTTAATAACATGTGTTCTTTCATGCAGAAGAAAAAAGCGATGAAGAGGAAAATTTGCCTTCCCTGGAAACTCTTGAAATTAGCAGGACCAAGATTGTTAAGTCAATTCCAAACTATTTTGGTGGTGAAGATGAAGATGGTATTCCTGACCTTGCTGATATTGAAGAAAATGATGACGCCCTTGCAACTGATGCAGTATGTTAATTTTTATGATCCAATTTATGAGCAAATGTGGCTAAAAAATACTCAACTTTATAGGAGGGCCTACATAACCACCGCCCTCCCACCCCCATCCCCATGATCTCTTAATTTGCAAGGCATGGATTGTGGAAAATAATCTAGAATCTTTCTTGGCATCTCAACTTGTAAAAGTTTTCTTGAGTCTGATGACGAAAGTTCTTGCTTCATTATCATCATGTACACATTTACCATTTGGGATTAAAAACTCTTTATGATCATCCTGCATTGTTGTGATTTTGTTAAGGTATTCATGTTCACATTTTCATTGTAGGCCACTCTGCCATTGACCTACCAAATTGCCCATGAACCTGATGACGATAATATCCTTCGAACACGAACTTATGATGTCAGCATCACGTAAGGCGGTCTCTTCTTTCTTGTTTTGTCTTTTTCGTAATTCTTCCAAAGATTCTTATAATGTATCTGTTGCATAAAAATGGATCAAAATTCTTGGGTTGGTGAAAAAAATCCTTCTTTCTAGCCGAAGAGGGAAGTTATGGCAAGAAAAATTGATGCAATTGGAGTAAATAGCATAGTAAGAGGGTAGTTAGAAATATCTCAGAAATGATCCCTATATGAAGCTTGAATTCAATTTGAGTTCTTCCATAATTATTTTCCTGTCTATAAAATTCTCCACTTTCTTCTACTCCAAATCTCTGAAGCACTGCAAAGACTGCCATTGGAAAGAGTTGTAACGGATTTGGATCAGATAGCCCAGATGCGGTTATTACTTTACGTTTGGTTTTCTTAGGCAGTCATTGTTATTGTTAATCACTTTGAAAATAATTATTAGCAAGTTCATTTGGAGCATTGGTGCACTACTTGGGGATTCAAATCTTTGCCGTTTTTGTCAAGTAGGAAATTATCACTGTCGGACTTGGGATTTTTTTCAGGGTTGGGCATATAGGTAATGTTCGTGGCTTCAACCTTAGTGGTCCTAGGTTTAAATCTTAAAAAATAGACATACTGGTTGATGATCTTGGCCTCAATGTGAGTGGTTCTGGCTTCTTGGCCCCGAAAACATTCTTGTGGCAAAAGGCTTAGGCTCAAAATGACAAAGGTGGATAAAAGGATGCATCTCATTTACAAACTTCTCTATCATCATCAATGGAAGACCGAGAGGTAAAATTTATGCCACATGGGGTCCCCGACAAGGAGATCGACTATCCCCTTTTCTTTTCATCATAGTAATGTATTGCCTCAGTTGCCTACTAACAAAAGCAGAATACGAAGGGCAAATAAAATGTTTTCAGATTGGTAATGAAGGCTTAAGCATCAACCACCTTCAATTCCGGACGACACAATACTCTTTTCTGACTTGGCAGATGCCTCTTCCATAAAAAACATGATTGAGATGGTAAAAACCTTTGAAGGATTCTCTGGACAAAATATCAATCTCCAAAAAAAACAAAAAGCATGGTCATCAATATTAGCACAGAGATTATTGAAGAATTTTCTAGCATATATGGTTGTATAAAGGGAGAATGATCGAATATGTACCTAGGACTACCTCTAAAAGGAAACCACGAATCTTTCTCCTTATGGGAGGTTATTATTGAAAAAATTGAAAGAAGGCTTTCAACATGGTCATCATGTTATACTTCAAAAGGTGGAAGACTCACCTTAATACAAGCTACATTATCAAACCTCCCCACTTACTGCGTGTCTCTATTCGAAATGCCCATCTACACCTTGTTCGATGGAATATTATAAACCTCCCAATAGAAAAGGGAGATCTTGATCTTTTCTCGATAAAGAAGAAGAACAAAGCCCTCCTCGCCAAATGGATATGGATATATTATCATGAAGAAAAGGCCTTGTGGAGAAATCTTATAAAGGCCAAATATACTCTTACTTCAAACAAAAATCAACCCCTCCATCTTCTACAAAAGGGCCTTGGAAGTACATAAGGAAACATCAAAACCTCATCACCAACTAAACTTGCCATAGGGTGGGTGATGGAGGAAGCACATCATTGTGGACCGACCCCTGGATTGAAAACACCATGCTAGCCCTGAGGTATCAACTTTTGTACAAATTCTCACATAGCAAAACAACAAGGATCAAAGAAACGTGGAATGTTGTCAACAAATTTTGGGACCTAAAGGGAATTATAATGAAGCAACGGAGTGTGCTGAATTAAGCCTTGACCTTGCCCCCGTGGTATTGTCAAATAAAGAAGACTCACTAACTTAGCTCCCTAGCACTGATAGGGTTCTCTACAAAATCCTTGATGATGGACATGGGGGAAAAAGTAGAAGCAATAAATACCATGCTAGCAAAGATAGTATGGAAAGGACATCATCCTAAAAAGATGAATTTTTTTCTTTGGAAAATAGCGCATAAAGCCATTACCACAAGTGAAAATCTTCGGAAAAAAAAGAAAAAAAAGGCTTATATCTCTCTTTCTCCAAATTGGTGTCCATTATGCGAGAAAGGAAGTGAATCACATAGCCACTTATTTATGCAATCACGTACGCTCTGAATTTCTGGACAACGATTCTCAATATATTCGAATGGCATCTCACATTTCTAGGGAGGTAAAGAGTCTATTGGACATGACCTTAATGTACCACCCTTTCAAGAATGCAAAAGCCCTACTATGAGAAAACCTCATCATGACTTTCTTTTGGAATCTGTGGAAAGAAAGAAATCAGAGAATATTTGCAGAAAAGACAAGACTTATACAAAACTTTTCGACAACGTTGTTTACCAAGCTATATCTTGGTGCAAACTGTCTAATATTTTTAATTCCTATAGTTATACCTCCCTCATTGCAAATTGGGAAGGTCTTTTGTAGACATCATGGATACTACGGCCCTTTTGTAAATTTCAATCTTCAATGAAATTGTATCCTATGTAAAGAAAAAAAAAAAAAATGCAAGTAAGGGAATAACCATAGAAAAGGAATATGCCAAAAATATGTCTGCAATAGCTAATCCTATAATACCATCTACTCTTTGGGACAAAGTGCCTATCATTATTGAATCATATCGTTTGTATGACGGGTCTAAACTTGTAATTTGATCAAATTTTTTTCTATCTAATGATGCTATTTTTCTCTTATATCAGGTATGATAAATATTATCAGACACCACGTGTTTGGCTGACAGGATATGATGAGGTTTTGTATTTTTATTCTGTTTCCATTTCTAGTTTGGTAGAACTTTTGATCTTGTACATATAATCACCATGCATGCATTCTATTGCTATGCAAGTTCTTTTTCTTACACAACTTCTATTAGCATGCACGTATGTACTTAAATCAAGTATCTGTGAACTATGTCAGTCAAGGATGCTCTTACAGCCAGAACATGTGCTTGAAGATGTTAGTCAAGATCATGCACGAAAAACGGTAAGTTGTGATTATGTAATAATGTAGTTGTGCCATCTGCAAGAAATAAATTGATCCTGATATTAGGCCCATCATTTATTCCATGTTATTTTTGTTGCAAGTCTAAATTAGCTTGCTGTTTTTCTTTTCCGTTTGAGAGAGGTACATTTACCTTTTATACTATAAAAAAAGGTTAAATCTATGAATTATGAATTATCAAATTTGGATCCTTGTGGATGCGATAATCTATAATTTTACACATTATTAAGATGACCAGCAAGAATCCCTCTTGATGCATCCTGAACTCCTATTGTGGTTTCTGGAGGACCCATGCATTTGTTTTATTGATTTGCCCTTCCAACGGTCATGTGGAACATGATTTTCCAATTATGTTATAAGTTCAATCTTTGGACACAGGTGACCATTGAGGACCATCCCCACCTACCTGGAAAGCATGCATCTGTACATCCCTGCAGACATGGGGCTGTGATGAAAAAGATCATTGATGTGTTGATGTCCCGGGGCGTAGAACCGGAAGTTCACAAGTAAGTATTATGAACTCCTATTTAAAACAGCAGATCTAGTTTTGGTGACAATCTTTGCTTGTTCCTAACCAAGTATCTCAAGCTCCTCTACTTATTAATTGTTTTCTTCTATCTTTTGTAGGTATCTTTTCTTGTTCTTAAAATTTGTCGCGTCTGTTATTCCGACTATCGAATATGATTATACTATGGACGTTGATCTTGGAGGCCCTAGCCGTCGATAACTGGGATTGATGAATATTGTAAGCACAACAGGATCGTAATTCCTTTTCAACTTCCGGCATCTTAACATGCTTGTGTACATCCAAAGTGCTGCTTATTATTACTTCTAGTGTTTATTTGTTGGAGTTTGGTTGTGGGTCAAGTAAAACGTACTATTTCCATTTTCTCAACAAAAGTGTGATTTCTTGCCAAAAGAAATAGGTCCATTGCTTGAGAAAATTCATGCACTACTCAATCAGTCGTACAATATACACTTAAATTTTTCTTTCAGAATACGTGGGTTTTAAAGGCTCAACTGTTTGCACTCCTTAACTATATATGCTTGCTAATGATGTAGTCCATTCGAAAAGTTTTAAAATCAGAGATATTATGGCGTGTTTTACTTTTTTTTTTTTTTTTTTTTGATCATTTGTGAATTTATAGCACTACTAATAGTTGGAATACCTGATATAGCCTGATCCATGACTCGTTGACTGACATTTCGTATATGGTTCTAACTATAAAACTATATGCTGGTAATGTTGGGGCACGCCTTAAAGTAAAGTGCTCCTCTCCAGGTCGACCATCGTCAACTAGTCAATAATAGACTGACATTTCTTTATTGTTTTCAATGGTTGTTACTCAAATCCGGGCTGACTCTTGAACATTTTTGAACTATGCAGGTCTGATTATACATATGTGGGGTATTTCACATCTGATGTTTCTGTAAATGCAGGCTGACACTCTGCTCATTGTACATACACAACCTGGGATCTAAATGGTACCCTCTGGAAATACTTTTGTCTTTTGGAAGTCTAATCTTCCCGTTGCTTTCTGTCAGTCTTAGTTTGGGCATAAGGATTCTTTGTGATTCTCTGTAACTTAACCATGGAGCTGACACTTGCTCCTTAACTGTATTCTGCTATATGGAAATTTGTTTGCTACTGAACTATTGCAGCAAAAAATGATTCCTCTTATTGGAAAAAGGAAGTGATTGAATATACCACTGTTGCTATAATACCTTTTAACAATCTCTGTTCAGGTTCTTGATTTTTATTTGGTTCCCTTGCAAATAAAACCTTCAAACCTTTAAATTTAGCTT

mRNA sequence

ATGGTTTTGTCGCAGAAGCTTCACGAGGCCTTCAAAGGAACTGTCGAGAGGATCACAGGGCCTCGAACCGTTTCCGCCTTCAAAGAGAAAGGCGTTCTCAGTGTCTCTGAGTTCATCGTCGCCGGTGATAATCTCGTCTCCAAGTGTCCCACCTGGTCTTGGGAGTCAGGTGATCTGAATAAGAGGAAATCATACTTGCCCCCAGATAAGCAATTCTTAATTACCAGAAATGTTCCTTGCCTTCGCCGGGCTGCCTATGTAGAAGAGGAGTATGAAGCTGCAGGTGGTGAAGTTTTACTTGATGAGGAAGATGGCGATGGCTGGCTGGCAACTCATGGCAAACCGAAGGAAGAAAAAAGCGATGAAGAGGAAAATTTGCCTTCCCTGGAAACTCTTGAAATTAGCAGGACCAAGATTGTTAAGTCAATTCCAAACTATTTTGGTGGTGAAGATGAAGATGGTATTCCTGACCTTGCTGATATTGAAGAAAATGATGACGCCCTTGCAACTGATGCAGCCACTCTGCCATTGACCTACCAAATTGCCCATGAACCTGATGACGATAATATCCTTCGAACACGAACTTATGATGTCAGCATCACGTATGATAAATATTATCAGACACCACGTGTTTGGCTGACAGGATATGATGAGTCAAGGATGCTCTTACAGCCAGAACATGTGCTTGAAGATGTTAGTCAAGATCATGCACGAAAAACGGTGACCATTGAGGACCATCCCCACCTACCTGGAAAGCATGCATCTGTACATCCCTGCAGACATGGGGCTGTGATGAAAAAGATCATTGATGTGTTGATGTCCCGGGGCGTAGAACCGGAAGTTCACAAGTATCTTTTCTTGTTCTTAAAATTTGTCGCGTCTGTTATTCCGACTATCGAATATGATTATACTATGGACGTTGATCTTGGAGGCCCTAGCCGTCGATAACTGGGATTGATGAATATTGTCTGATTATACATATGTGGGGTATTTCACATCTGATGTTTCTGTAAATGCAGGCTGACACTCTGCTCATTGTACATACACAACCTGGGATCTAAATGGTACCCTCTGGAAATACTTTTGTCTTTTGGAAGTCTAATCTTCCCGTTGCTTTCTGTCAGTCTTAGTTTGGGCATAAGGATTCTTTGTGATTCTCTGTAACTTAACCATGGAGCTGACACTTGCTCCTTAACTGTATTCTGCTATATGGAAATTTGTTTGCTACTGAACTATTGCAGCAAAAAATGATTCCTCTTATTGGAAAAAGGAAGTGATTGAATATACCACTGTTGCTATAATACCTTTTAACAATCTCTGTTCAGGTTCTTGATTTTTATTTGGTTCCCTTGCAAATAAAACCTTCAAACCTTTAAATTTAGCTT

Coding sequence (CDS)

ATGGTTTTGTCGCAGAAGCTTCACGAGGCCTTCAAAGGAACTGTCGAGAGGATCACAGGGCCTCGAACCGTTTCCGCCTTCAAAGAGAAAGGCGTTCTCAGTGTCTCTGAGTTCATCGTCGCCGGTGATAATCTCGTCTCCAAGTGTCCCACCTGGTCTTGGGAGTCAGGTGATCTGAATAAGAGGAAATCATACTTGCCCCCAGATAAGCAATTCTTAATTACCAGAAATGTTCCTTGCCTTCGCCGGGCTGCCTATGTAGAAGAGGAGTATGAAGCTGCAGGTGGTGAAGTTTTACTTGATGAGGAAGATGGCGATGGCTGGCTGGCAACTCATGGCAAACCGAAGGAAGAAAAAAGCGATGAAGAGGAAAATTTGCCTTCCCTGGAAACTCTTGAAATTAGCAGGACCAAGATTGTTAAGTCAATTCCAAACTATTTTGGTGGTGAAGATGAAGATGGTATTCCTGACCTTGCTGATATTGAAGAAAATGATGACGCCCTTGCAACTGATGCAGCCACTCTGCCATTGACCTACCAAATTGCCCATGAACCTGATGACGATAATATCCTTCGAACACGAACTTATGATGTCAGCATCACGTATGATAAATATTATCAGACACCACGTGTTTGGCTGACAGGATATGATGAGTCAAGGATGCTCTTACAGCCAGAACATGTGCTTGAAGATGTTAGTCAAGATCATGCACGAAAAACGGTGACCATTGAGGACCATCCCCACCTACCTGGAAAGCATGCATCTGTACATCCCTGCAGACATGGGGCTGTGATGAAAAAGATCATTGATGTGTTGATGTCCCGGGGCGTAGAACCGGAAGTTCACAAGTATCTTTTCTTGTTCTTAAAATTTGTCGCGTCTGTTATTCCGACTATCGAATATGATTATACTATGGACGTTGATCTTGGAGGCCCTAGCCGTCGATAA

Protein sequence

MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWESGDLNKRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEEDGDGWLATHGKPKEEKSDEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEENDDALATDAATLPLTYQIAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPEHVLEDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIEYDYTMDVDLGGPSRR
Homology
BLAST of Carg04976 vs. NCBI nr
Match: KAG7025347.1 (Autophagy-related protein 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 643.7 bits (1659), Expect = 8.5e-181
Identity = 315/315 (100.00%), Postives = 315/315 (100.00%), Query Frame = 0

Query: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWESGDLN 60
           MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWESGDLN
Sbjct: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWESGDLN 60

Query: 61  KRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEEDGDGWLATHGKPKEEKS 120
           KRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEEDGDGWLATHGKPKEEKS
Sbjct: 61  KRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEEDGDGWLATHGKPKEEKS 120

Query: 121 DEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEENDDALATDAATLPLTYQ 180
           DEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEENDDALATDAATLPLTYQ
Sbjct: 121 DEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEENDDALATDAATLPLTYQ 180

Query: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPEHVLEDVSQDHARKT 240
           IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPEHVLEDVSQDHARKT
Sbjct: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPEHVLEDVSQDHARKT 240

Query: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300
           VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE
Sbjct: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300

Query: 301 YDYTMDVDLGGPSRR 316
           YDYTMDVDLGGPSRR
Sbjct: 301 YDYTMDVDLGGPSRR 315

BLAST of Carg04976 vs. NCBI nr
Match: XP_023005051.1 (autophagy-related protein 3-like [Cucurbita maxima])

HSP 1 Score: 643.3 bits (1658), Expect = 1.1e-180
Identity = 314/315 (99.68%), Postives = 315/315 (100.00%), Query Frame = 0

Query: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWESGDLN 60
           MVLSQKLHEAFKGTVERITGPRT+SAFKEKGVLSVSEFIVAGDNLVSKCPTWSWESGDLN
Sbjct: 1   MVLSQKLHEAFKGTVERITGPRTISAFKEKGVLSVSEFIVAGDNLVSKCPTWSWESGDLN 60

Query: 61  KRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEEDGDGWLATHGKPKEEKS 120
           KRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEEDGDGWLATHGKPKEEKS
Sbjct: 61  KRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEEDGDGWLATHGKPKEEKS 120

Query: 121 DEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEENDDALATDAATLPLTYQ 180
           DEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEENDDALATDAATLPLTYQ
Sbjct: 121 DEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEENDDALATDAATLPLTYQ 180

Query: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPEHVLEDVSQDHARKT 240
           IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPEHVLEDVSQDHARKT
Sbjct: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPEHVLEDVSQDHARKT 240

Query: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300
           VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE
Sbjct: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300

Query: 301 YDYTMDVDLGGPSRR 316
           YDYTMDVDLGGPSRR
Sbjct: 301 YDYTMDVDLGGPSRR 315

BLAST of Carg04976 vs. NCBI nr
Match: XP_023514388.1 (autophagy-related protein 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 642.5 bits (1656), Expect = 1.9e-180
Identity = 314/315 (99.68%), Postives = 315/315 (100.00%), Query Frame = 0

Query: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWESGDLN 60
           MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWESGDLN
Sbjct: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWESGDLN 60

Query: 61  KRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEEDGDGWLATHGKPKEEKS 120
           KRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEEDGDGWLATHGKPKEEKS
Sbjct: 61  KRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEEDGDGWLATHGKPKEEKS 120

Query: 121 DEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEENDDALATDAATLPLTYQ 180
           DEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADI+ENDDALATDAATLPLTYQ
Sbjct: 121 DEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIDENDDALATDAATLPLTYQ 180

Query: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPEHVLEDVSQDHARKT 240
           IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPEHVLEDVSQDHARKT
Sbjct: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPEHVLEDVSQDHARKT 240

Query: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300
           VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE
Sbjct: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300

Query: 301 YDYTMDVDLGGPSRR 316
           YDYTMDVDLGGPSRR
Sbjct: 301 YDYTMDVDLGGPSRR 315

BLAST of Carg04976 vs. NCBI nr
Match: XP_022959612.1 (autophagy-related protein 3-like [Cucurbita moschata])

HSP 1 Score: 641.7 bits (1654), Expect = 3.2e-180
Identity = 314/315 (99.68%), Postives = 314/315 (99.68%), Query Frame = 0

Query: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWESGDLN 60
           MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWESGDLN
Sbjct: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWESGDLN 60

Query: 61  KRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEEDGDGWLATHGKPKEEKS 120
           KRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEEDGDGWLATHGKPKEEKS
Sbjct: 61  KRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEEDGDGWLATHGKPKEEKS 120

Query: 121 DEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEENDDALATDAATLPLTYQ 180
           DEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEENDDALATDAATLPLTYQ
Sbjct: 121 DEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEENDDALATDAATLPLTYQ 180

Query: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPEHVLEDVSQDHARKT 240
           IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPEHVLEDVSQDHARKT
Sbjct: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPEHVLEDVSQDHARKT 240

Query: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300
           VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE
Sbjct: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300

Query: 301 YDYTMDVDLGGPSRR 316
           YDYTMDVDLGGPS R
Sbjct: 301 YDYTMDVDLGGPSHR 315

BLAST of Carg04976 vs. NCBI nr
Match: KAG7013823.1 (Autophagy-related protein 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 611.7 bits (1576), Expect = 3.6e-171
Identity = 297/315 (94.29%), Postives = 304/315 (96.51%), Query Frame = 0

Query: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWESGDLN 60
           M LSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFI+AGDNLVSKCPTWSWESGD+N
Sbjct: 1   MALSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIIAGDNLVSKCPTWSWESGDIN 60

Query: 61  KRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEEDGDGWLATHGKPKEEKS 120
           KRKSYLPPDKQFLITRNVPCLRRAA VEEEYEAAGGEVLLD EDGDGWLATHGKPKEEKS
Sbjct: 61  KRKSYLPPDKQFLITRNVPCLRRAASVEEEYEAAGGEVLLDNEDGDGWLATHGKPKEEKS 120

Query: 121 DEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEENDDALATDAATLPLTYQ 180
           DEEENLPSLETLEISR K+VKSIPNYFGGEDE+ IPDLADIE+ D+ + TDAATLPLTYQ
Sbjct: 121 DEEENLPSLETLEISRPKVVKSIPNYFGGEDEEDIPDLADIEDTDNVIGTDAATLPLTYQ 180

Query: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPEHVLEDVSQDHARKT 240
           IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPE VLEDVSQDHARKT
Sbjct: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPEFVLEDVSQDHARKT 240

Query: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300
           VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE
Sbjct: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300

Query: 301 YDYTMDVDLGGPSRR 316
           YDYTMD DLGGPS R
Sbjct: 301 YDYTMDFDLGGPSSR 315

BLAST of Carg04976 vs. ExPASy Swiss-Prot
Match: Q0WWQ1 (Autophagy-related protein 3 OS=Arabidopsis thaliana OX=3702 GN=ATG3 PE=1 SV=2)

HSP 1 Score: 533.5 bits (1373), Expect = 1.6e-150
Identity = 256/313 (81.79%), Postives = 282/313 (90.10%), Query Frame = 0

Query: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWESGDLN 60
           MVLSQKLHEAFKGTVERITGPRT+SAFKEKGVLSVSEF++AGDNLVSKCPTWSWESGD +
Sbjct: 1   MVLSQKLHEAFKGTVERITGPRTISAFKEKGVLSVSEFVLAGDNLVSKCPTWSWESGDAS 60

Query: 61  KRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEEDGDGWLATHGKPKEEKS 120
           KRK YLP DKQFLITRNVPCLRRAA V E+YEAAGGEVL+D+ED DGWLATHGKPK +K 
Sbjct: 61  KRKPYLPSDKQFLITRNVPCLRRAASVAEDYEAAGGEVLVDDEDNDGWLATHGKPK-DKG 120

Query: 121 DEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEENDDALATDAATLPLTYQ 180
            EE+NLPS++ L+I+    ++SIP YFGGE++D IPD+ + +E D+ +  D ATL  TY 
Sbjct: 121 KEEDNLPSMDALDINEKNTIQSIPTYFGGEEDDDIPDMEEFDEADNVVENDPATLQSTYL 180

Query: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPEHVLEDVSQDHARKT 240
           +AHEPDDDNILRTRTYD+SITYDKYYQTPRVWLTGYDESRMLLQPE V+EDVSQDHARKT
Sbjct: 181 VAHEPDDDNILRTRTYDLSITYDKYYQTPRVWLTGYDESRMLLQPELVMEDVSQDHARKT 240

Query: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300
           VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEV KYLFLFLKF+ASVIPTIE
Sbjct: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVDKYLFLFLKFMASVIPTIE 300

Query: 301 YDYTMDVDLGGPS 314
           YDYTMD DLG  S
Sbjct: 301 YDYTMDFDLGSSS 312

BLAST of Carg04976 vs. ExPASy Swiss-Prot
Match: Q550A8 (Autophagy-related protein 3 OS=Dictyostelium discoideum OX=44689 GN=atg3 PE=3 SV=3)

HSP 1 Score: 287.0 bits (733), Expect = 2.7e-76
Identity = 159/333 (47.75%), Postives = 207/333 (62.16%), Query Frame = 0

Query: 5   QKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWESGDLNKRKS 64
           Q +H+A+  TVE++T   + S F E+GVL+  EF+ AGD L  KC TW+WESGD ++  S
Sbjct: 7   QAVHKAYVKTVEKVTPTLSTSKFLEEGVLTPEEFVQAGDLLTDKCQTWTWESGDPSRNVS 66

Query: 65  YLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEEDG-DGWLATHGKPKEEKSDEE 124
           YLP +KQFL+TRNVPC  R   +E E +A+  + +  E+DG D W+A   +P   + D E
Sbjct: 67  YLPKEKQFLLTRNVPCYNRVRTLENESKASKADEIQIEDDGEDSWVAP--QPVGNQDDIE 126

Query: 125 ENLPSLETLEI-------SRTKIVKSIPNYFGG-----EDEDG-IPDLAD------IEEN 184
           +    + +L+I       + T   K   N         EDEDG IPDL D      IEE 
Sbjct: 127 DEKVDISSLKIDDKKPNTTTTTTAKPTNNNNNNNDDEDEDEDGDIPDLDDFQDDNIIEEE 186

Query: 185 DDALATDAATLPLTYQIAHE---------PDDDNILRTRTYDVSITYDKYYQTPRVWLTG 244
           D A+ +       T    +           D+DNILRTRTYD+SITYDKYYQTPRVWL G
Sbjct: 187 DPAVLSKNNKTTTTTTANNNNNNNSENKVEDNDNILRTRTYDISITYDKYYQTPRVWLFG 246

Query: 245 YDESRMLLQPEHVLEDVSQDHARKTVTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSR 304
           YDE+R  L+PE + ED+S+DHA KTVTI+ HPHL    A +HPCRH AVMKK++D     
Sbjct: 247 YDENRKPLKPEEIFEDISEDHAHKTVTIDSHPHLGISFAYIHPCRHAAVMKKLVDRQSEN 306

Query: 305 GVEPEVHKYLFLFLKFVASVIPTIEYDYTMDVD 309
           G EP V +YLFLFLKF++ VIPTIEYD+T++ D
Sbjct: 307 GKEPRVDQYLFLFLKFISVVIPTIEYDFTLEFD 337

BLAST of Carg04976 vs. ExPASy Swiss-Prot
Match: Q9CPX6 (Ubiquitin-like-conjugating enzyme ATG3 OS=Mus musculus OX=10090 GN=Atg3 PE=1 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 2.9e-67
Identity = 143/297 (48.15%), Postives = 185/297 (62.29%), Query Frame = 0

Query: 16  ERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWESGDLNKRKSYLPPDKQFLIT 75
           E +T     S FKE GV++  EF+ AGD+LV  CPTW W +G+  K K+YLP DKQFL+T
Sbjct: 17  EYLTPVLKESKFKETGVITPEEFVAAGDHLVHHCPTWQWATGEELKVKAYLPTDKQFLVT 76

Query: 76  RNVPCLRRAAYVEEEYEAAGGEVLLDEEDGD-GWLATHGKPKEEKSDEEENLPSLETLEI 135
           +NVPC +R   +E   E    E +++E+DGD GW+ T+         E     +LE+ + 
Sbjct: 77  KNVPCYKRCKQMEYSDEL---EAIIEEDDGDGGWVDTYHNTGITGITEAVKEITLESKDS 136

Query: 136 SRTKIVKSIPNYFGGEDEDGIPDLADIEENDDALATDAATLPLTYQI------AHEPDDD 195
            + +   ++ +    EDE    D+ + EE+   L TD ATL     +      A    +D
Sbjct: 137 IKLQDCSALCDEEDEEDEGEAADMEEYEES-GLLETDEATLDTRKIVEACKAKADAGGED 196

Query: 196 NILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPEHVLEDVSQDHARKTVTIEDHPH 255
            IL+TRTYD+ ITYDKYYQTPR+WL GYDE R  L  EH+ ED+SQDH +KTVTIE+HPH
Sbjct: 197 AILQTRTYDLYITYDKYYQTPRLWLFGYDEQRQPLTVEHMYEDISQDHVKKTVTIENHPH 256

Query: 256 L-PGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIEYDYT 305
           L P    SVHPCRH  VMKKII+ +   G E  VH YL +FLKFV +VIPTIEYDYT
Sbjct: 257 LPPPPMCSVHPCRHAEVMKKIIETVAEGGGELGVHMYLLIFLKFVQAVIPTIEYDYT 309

BLAST of Carg04976 vs. ExPASy Swiss-Prot
Match: Q6PFS7 (Ubiquitin-like-conjugating enzyme ATG3 OS=Danio rerio OX=7955 GN=atg3 PE=2 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 3.8e-67
Identity = 145/302 (48.01%), Postives = 184/302 (60.93%), Query Frame = 0

Query: 13  GTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWESGDLNKRKSYLPPDKQF 72
           G  E +T     S FKE GV++  EF+ AGD+LV  CPTW W SG+  K K YLP DKQF
Sbjct: 14  GVAEFLTPVLKESKFKETGVITPEEFVAAGDHLVHHCPTWKWASGEEAKVKPYLPNDKQF 73

Query: 73  LITRNVPCLRRAAYVEEEYEAAGGEVLLDEEDGD-GWLATHGKPKEEKSDEEENLPSLET 132
           L+TRNVPC +R   +E   E    E +++E+DGD GW+ T          E     SL+ 
Sbjct: 74  LLTRNVPCYKRCKQMEYSDEL---EAIIEEDDGDGGWVDTFHNSGVTGVTEAVREISLDN 133

Query: 133 LEISRTKIVKSIPNYFGGEDEDGIPDLADIEENDDA--LATDAATL------PLTYQIAH 192
            +     +        G +D+D   + AD+EE +++  L TD ATL       L+   A 
Sbjct: 134 KDNMNMNVKTGACGNSGDDDDDEEGEAADMEEYEESGLLETDDATLDTSKMADLSKTKAE 193

Query: 193 EPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPEHVLEDVSQDHARKTVTI 252
              +D IL+TRTYD+ ITYDKYYQTPR+WL GYDE R  L  + + ED+SQDH +KTVTI
Sbjct: 194 AGGEDAILQTRTYDLYITYDKYYQTPRLWLFGYDEDRQPLTVDQMYEDISQDHVKKTVTI 253

Query: 253 EDHPHL-PGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIEYD 305
           E+HP+L P    SVHPCRH  VMKKII+ +   G E  VH YL +FLKFV +VIPTIEYD
Sbjct: 254 ENHPNLPPPAMCSVHPCRHAEVMKKIIETVAEGGGELGVHMYLLIFLKFVQAVIPTIEYD 312

BLAST of Carg04976 vs. ExPASy Swiss-Prot
Match: Q6AZ50 (Ubiquitin-like-conjugating enzyme ATG3 OS=Rattus norvegicus OX=10116 GN=Atg3 PE=2 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 4.2e-66
Identity = 143/302 (47.35%), Postives = 191/302 (63.25%), Query Frame = 0

Query: 16  ERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWESGDLNKRKSYLPPDKQFLIT 75
           E +T     S FKE GV++  EF+ AGD+LV  CPTW W +G+  K K+YLP  KQFL+T
Sbjct: 17  EYLTPVLKESKFKETGVITPEEFVAAGDHLVHHCPTWQWATGEELKVKAYLPTGKQFLVT 76

Query: 76  RNVPCLRRAAYVEEEYEAAGGEVLLDEEDGD-GWLATH---GKPKEEKSDEEENLPSLET 135
           +NVPC +R   +E   E    E +++E+DGD GW+ T+   G     ++ +E  L S ++
Sbjct: 77  KNVPCYKRCKQMEYSDEL---EAIIEEDDGDGGWVDTYHNTGITGITEAVKEITLESKDS 136

Query: 136 LEISRTKIVKSIPNYFGGEDEDGIPDLADIEENDDA--LATDAATLPLTYQI------AH 195
           +++    ++         E+E+   + AD+EE +++  L TD ATL     +      A 
Sbjct: 137 IKLQDCSVL------CDEEEEEEEGEAADMEEYEESGLLETDEATLDTRRIVEACKAKAD 196

Query: 196 EPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPEHVLEDVSQDHARKTVTI 255
              +D IL+TRTYD+ ITYDKYYQTPR+WL GYDE R  L  EH+ ED+SQDH +KTVTI
Sbjct: 197 AGGEDAILQTRTYDLYITYDKYYQTPRLWLFGYDEQRQPLTVEHMYEDISQDHVKKTVTI 256

Query: 256 EDHPHL-PGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIEYD 305
           E+HPHL P    SVHPCRH  VMKKII+ +   G E  VH YL +FLKFV +VIPTIEYD
Sbjct: 257 ENHPHLPPPPMCSVHPCRHAEVMKKIIETVAEGGGELGVHMYLLIFLKFVQAVIPTIEYD 309

BLAST of Carg04976 vs. ExPASy TrEMBL
Match: A0A6J1KTV3 (autophagy-related protein 3-like OS=Cucurbita maxima OX=3661 GN=LOC111498158 PE=4 SV=1)

HSP 1 Score: 643.3 bits (1658), Expect = 5.4e-181
Identity = 314/315 (99.68%), Postives = 315/315 (100.00%), Query Frame = 0

Query: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWESGDLN 60
           MVLSQKLHEAFKGTVERITGPRT+SAFKEKGVLSVSEFIVAGDNLVSKCPTWSWESGDLN
Sbjct: 1   MVLSQKLHEAFKGTVERITGPRTISAFKEKGVLSVSEFIVAGDNLVSKCPTWSWESGDLN 60

Query: 61  KRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEEDGDGWLATHGKPKEEKS 120
           KRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEEDGDGWLATHGKPKEEKS
Sbjct: 61  KRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEEDGDGWLATHGKPKEEKS 120

Query: 121 DEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEENDDALATDAATLPLTYQ 180
           DEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEENDDALATDAATLPLTYQ
Sbjct: 121 DEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEENDDALATDAATLPLTYQ 180

Query: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPEHVLEDVSQDHARKT 240
           IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPEHVLEDVSQDHARKT
Sbjct: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPEHVLEDVSQDHARKT 240

Query: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300
           VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE
Sbjct: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300

Query: 301 YDYTMDVDLGGPSRR 316
           YDYTMDVDLGGPSRR
Sbjct: 301 YDYTMDVDLGGPSRR 315

BLAST of Carg04976 vs. ExPASy TrEMBL
Match: A0A6J1H511 (autophagy-related protein 3-like OS=Cucurbita moschata OX=3662 GN=LOC111460636 PE=4 SV=1)

HSP 1 Score: 641.7 bits (1654), Expect = 1.6e-180
Identity = 314/315 (99.68%), Postives = 314/315 (99.68%), Query Frame = 0

Query: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWESGDLN 60
           MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWESGDLN
Sbjct: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWESGDLN 60

Query: 61  KRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEEDGDGWLATHGKPKEEKS 120
           KRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEEDGDGWLATHGKPKEEKS
Sbjct: 61  KRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEEDGDGWLATHGKPKEEKS 120

Query: 121 DEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEENDDALATDAATLPLTYQ 180
           DEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEENDDALATDAATLPLTYQ
Sbjct: 121 DEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEENDDALATDAATLPLTYQ 180

Query: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPEHVLEDVSQDHARKT 240
           IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPEHVLEDVSQDHARKT
Sbjct: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPEHVLEDVSQDHARKT 240

Query: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300
           VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE
Sbjct: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300

Query: 301 YDYTMDVDLGGPSRR 316
           YDYTMDVDLGGPS R
Sbjct: 301 YDYTMDVDLGGPSHR 315

BLAST of Carg04976 vs. ExPASy TrEMBL
Match: A0A1S3CAI3 (autophagy-related protein 3 OS=Cucumis melo OX=3656 GN=LOC103498815 PE=4 SV=1)

HSP 1 Score: 610.9 bits (1574), Expect = 3.0e-171
Identity = 297/313 (94.89%), Postives = 303/313 (96.81%), Query Frame = 0

Query: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWESGDLN 60
           MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFI+AGDNLVSKCPTWSWESGDLN
Sbjct: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIIAGDNLVSKCPTWSWESGDLN 60

Query: 61  KRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEEDGDGWLATHGKPKEEKS 120
           KRKSYLPPDKQFLITRNVPCLRRAA +EEEYEAAGGEVLLD EDGDGWLATHGKPKEEKS
Sbjct: 61  KRKSYLPPDKQFLITRNVPCLRRAASIEEEYEAAGGEVLLDNEDGDGWLATHGKPKEEKS 120

Query: 121 DEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEENDDALATDAATLPLTYQ 180
           DEEENLPSLE LEISRTK+VKSIPNYFGGEDED +PDL DIEE D+ + TDAATLPLTYQ
Sbjct: 121 DEEENLPSLEALEISRTKVVKSIPNYFGGEDEDDVPDLDDIEETDNVVGTDAATLPLTYQ 180

Query: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPEHVLEDVSQDHARKT 240
           IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPE VLEDVSQDHARKT
Sbjct: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKT 240

Query: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300
           VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE
Sbjct: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300

Query: 301 YDYTMDVDLGGPS 314
           YDYTMD DLGGPS
Sbjct: 301 YDYTMDFDLGGPS 313

BLAST of Carg04976 vs. ExPASy TrEMBL
Match: A0A6J1H4I9 (autophagy-related protein 3-like OS=Cucurbita moschata OX=3662 GN=LOC111460417 PE=4 SV=1)

HSP 1 Score: 610.9 bits (1574), Expect = 3.0e-171
Identity = 297/315 (94.29%), Postives = 304/315 (96.51%), Query Frame = 0

Query: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWESGDLN 60
           M LSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFI+AGDNLVSKCPTWSWESGD+N
Sbjct: 1   MALSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIIAGDNLVSKCPTWSWESGDIN 60

Query: 61  KRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEEDGDGWLATHGKPKEEKS 120
           KRKSYLPPDKQFLITRNVPCLRRAA VEEEYEAAGGEVLLD EDGDGWLATHGKPKEEKS
Sbjct: 61  KRKSYLPPDKQFLITRNVPCLRRAASVEEEYEAAGGEVLLDNEDGDGWLATHGKPKEEKS 120

Query: 121 DEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEENDDALATDAATLPLTYQ 180
           DEEENLPSLETLEISR K+VKSIPNYFGGEDE+ IPDLADIE+ D+ + TDAATLPLTYQ
Sbjct: 121 DEEENLPSLETLEISRPKVVKSIPNYFGGEDEEDIPDLADIEDTDNVIGTDAATLPLTYQ 180

Query: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPEHVLEDVSQDHARKT 240
           IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPE VLEDVSQDHARKT
Sbjct: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKT 240

Query: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300
           VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE
Sbjct: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300

Query: 301 YDYTMDVDLGGPSRR 316
           YDYTMD DLGGPS R
Sbjct: 301 YDYTMDFDLGGPSSR 315

BLAST of Carg04976 vs. ExPASy TrEMBL
Match: A0A5A7T8K5 (Autophagy-related protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold122G00500 PE=4 SV=1)

HSP 1 Score: 610.9 bits (1574), Expect = 3.0e-171
Identity = 297/313 (94.89%), Postives = 303/313 (96.81%), Query Frame = 0

Query: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWESGDLN 60
           MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFI+AGDNLVSKCPTWSWESGDLN
Sbjct: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIIAGDNLVSKCPTWSWESGDLN 60

Query: 61  KRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEEDGDGWLATHGKPKEEKS 120
           KRKSYLPPDKQFLITRNVPCLRRAA +EEEYEAAGGEVLLD EDGDGWLATHGKPKEEKS
Sbjct: 61  KRKSYLPPDKQFLITRNVPCLRRAASIEEEYEAAGGEVLLDNEDGDGWLATHGKPKEEKS 120

Query: 121 DEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEENDDALATDAATLPLTYQ 180
           DEEENLPSLE LEISRTK+VKSIPNYFGGEDED +PDL DIEE D+ + TDAATLPLTYQ
Sbjct: 121 DEEENLPSLEALEISRTKVVKSIPNYFGGEDEDDVPDLDDIEETDNVVGTDAATLPLTYQ 180

Query: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPEHVLEDVSQDHARKT 240
           IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPE VLEDVSQDHARKT
Sbjct: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPELVLEDVSQDHARKT 240

Query: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300
           VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE
Sbjct: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300

Query: 301 YDYTMDVDLGGPS 314
           YDYTMD DLGGPS
Sbjct: 301 YDYTMDFDLGGPS 313

BLAST of Carg04976 vs. TAIR 10
Match: AT5G61500.1 (autophagy 3 (APG3) )

HSP 1 Score: 533.5 bits (1373), Expect = 1.2e-151
Identity = 256/313 (81.79%), Postives = 282/313 (90.10%), Query Frame = 0

Query: 1   MVLSQKLHEAFKGTVERITGPRTVSAFKEKGVLSVSEFIVAGDNLVSKCPTWSWESGDLN 60
           MVLSQKLHEAFKGTVERITGPRT+SAFKEKGVLSVSEF++AGDNLVSKCPTWSWESGD +
Sbjct: 1   MVLSQKLHEAFKGTVERITGPRTISAFKEKGVLSVSEFVLAGDNLVSKCPTWSWESGDAS 60

Query: 61  KRKSYLPPDKQFLITRNVPCLRRAAYVEEEYEAAGGEVLLDEEDGDGWLATHGKPKEEKS 120
           KRK YLP DKQFLITRNVPCLRRAA V E+YEAAGGEVL+D+ED DGWLATHGKPK +K 
Sbjct: 61  KRKPYLPSDKQFLITRNVPCLRRAASVAEDYEAAGGEVLVDDEDNDGWLATHGKPK-DKG 120

Query: 121 DEEENLPSLETLEISRTKIVKSIPNYFGGEDEDGIPDLADIEENDDALATDAATLPLTYQ 180
            EE+NLPS++ L+I+    ++SIP YFGGE++D IPD+ + +E D+ +  D ATL  TY 
Sbjct: 121 KEEDNLPSMDALDINEKNTIQSIPTYFGGEEDDDIPDMEEFDEADNVVENDPATLQSTYL 180

Query: 181 IAHEPDDDNILRTRTYDVSITYDKYYQTPRVWLTGYDESRMLLQPEHVLEDVSQDHARKT 240
           +AHEPDDDNILRTRTYD+SITYDKYYQTPRVWLTGYDESRMLLQPE V+EDVSQDHARKT
Sbjct: 181 VAHEPDDDNILRTRTYDLSITYDKYYQTPRVWLTGYDESRMLLQPELVMEDVSQDHARKT 240

Query: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVHKYLFLFLKFVASVIPTIE 300
           VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEV KYLFLFLKF+ASVIPTIE
Sbjct: 241 VTIEDHPHLPGKHASVHPCRHGAVMKKIIDVLMSRGVEPEVDKYLFLFLKFMASVIPTIE 300

Query: 301 YDYTMDVDLGGPS 314
           YDYTMD DLG  S
Sbjct: 301 YDYTMDFDLGSSS 312

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7025347.18.5e-181100.00Autophagy-related protein 3, partial [Cucurbita argyrosperma subsp. argyrosperma... [more]
XP_023005051.11.1e-18099.68autophagy-related protein 3-like [Cucurbita maxima][more]
XP_023514388.11.9e-18099.68autophagy-related protein 3-like [Cucurbita pepo subsp. pepo][more]
XP_022959612.13.2e-18099.68autophagy-related protein 3-like [Cucurbita moschata][more]
KAG7013823.13.6e-17194.29Autophagy-related protein 3 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
Q0WWQ11.6e-15081.79Autophagy-related protein 3 OS=Arabidopsis thaliana OX=3702 GN=ATG3 PE=1 SV=2[more]
Q550A82.7e-7647.75Autophagy-related protein 3 OS=Dictyostelium discoideum OX=44689 GN=atg3 PE=3 SV... [more]
Q9CPX62.9e-6748.15Ubiquitin-like-conjugating enzyme ATG3 OS=Mus musculus OX=10090 GN=Atg3 PE=1 SV=... [more]
Q6PFS73.8e-6748.01Ubiquitin-like-conjugating enzyme ATG3 OS=Danio rerio OX=7955 GN=atg3 PE=2 SV=1[more]
Q6AZ504.2e-6647.35Ubiquitin-like-conjugating enzyme ATG3 OS=Rattus norvegicus OX=10116 GN=Atg3 PE=... [more]
Match NameE-valueIdentityDescription
A0A6J1KTV35.4e-18199.68autophagy-related protein 3-like OS=Cucurbita maxima OX=3661 GN=LOC111498158 PE=... [more]
A0A6J1H5111.6e-18099.68autophagy-related protein 3-like OS=Cucurbita moschata OX=3662 GN=LOC111460636 P... [more]
A0A1S3CAI33.0e-17194.89autophagy-related protein 3 OS=Cucumis melo OX=3656 GN=LOC103498815 PE=4 SV=1[more]
A0A6J1H4I93.0e-17194.29autophagy-related protein 3-like OS=Cucurbita moschata OX=3662 GN=LOC111460417 P... [more]
A0A5A7T8K53.0e-17194.89Autophagy-related protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf... [more]
Match NameE-valueIdentityDescription
AT5G61500.11.2e-15181.79autophagy 3 (APG3) [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.1460.50coord: 160..298
e-value: 3.1E-5
score: 25.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 105..126
NoneNo IPR availablePANTHERPTHR12866:SF2UBIQUITIN-LIKE-CONJUGATING ENZYME ATG3coord: 1..309
IPR007135Ubiquitin-like-conjugating enzyme Atg3/Atg10PFAMPF03987Autophagy_act_Ccoord: 37..303
e-value: 7.8E-65
score: 218.7
IPR007135Ubiquitin-like-conjugating enzyme Atg3/Atg10PANTHERPTHR12866UBIQUITIN-LIKE-CONJUGATING ENZYME ATG3coord: 1..309

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg04976-RACarg04976-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000045 autophagosome assembly
biological_process GO:0000422 autophagy of mitochondrion
biological_process GO:0044804 autophagy of nucleus
cellular_component GO:0000153 cytoplasmic ubiquitin ligase complex
cellular_component GO:0005829 cytosol
molecular_function GO:0019776 Atg8 ligase activity
molecular_function GO:0019787 ubiquitin-like protein transferase activity