Carg04941 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg04941
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionaspartyl protease family protein 2
LocationCarg_Chr08: 568777 .. 570183 (+)
RNA-Seq ExpressionCarg04941
SyntenyCarg04941
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTCTCCTCCAAGATATCTCCTCTTCTTCTTCTTCTTCTTCTTCGCCGCCGTGGCCTCCGCCGCGTCGGAGTTCCAAACCCTTACTCTCCGCCGTCTTCCAATTCCCTCTCCCCTTCCCTTTCCACAACCCCAATTCGACACCCAAGAAACGCTCGAATCCACCGCCGCCCTCACGGTTGAGCTCCACCATTTGGATTCACTCTCTACCAACAAAACCCCCTCCGATCTCTTCAACCTCCGGCTCCACCGCGACGCCCTCCGTGTTGACTCGTTGACCTCCCTGACTGCTGGCCGGAGCCGGACTCCTCTCCGGCGAGCCGGGTTCAGTAGCTCTGTTATCTCCGGCCTTGCTCAAGGTAGCGGTGAGTACTTCACCCGCCTTGGCGTCGGAACGCCTCCTAGATATATCTTTATGGTCCTCGACACCGGAAGCGACGTCGTTTGGCTTCAATGCTCCCCTTGCCGGAAATGCTACTCCCAATCTGATCCCATTTTTAACCCCTTCAAATCCAAATCCTTCGCCGGAATCCCCTGTTCTTCACCTCTCTGCCGCCGCCTTGACTCCTCCGGCTGCAACACTCGCCGCCACACCTGCCTCTACCAAGTCTCCTACGGCGATGGGTCCTTTACCACCGGCGATTTCGCCACCGAAACGCTCACGTTTCGTGGGAATAAAATCGCCAAAGTCGCCCTCGGCTGCGGCCACGACAATGAAGGCCTCTTCGTCGGTGCCGCTGGATTATTGGGTCTTGGCCGTGGTCGGTTTTCTTTCCCTTCTCAAACCGGACTCCGGTTCAACCATAAATTTTCTTATTGTTTGGTGGACCGGTCCGCTTCCTCCAAACCCTCCTCTATGGTTTTCGGTGATGCGGCAATTTCCCGGCTCGCCCGGTTCACTCCTCTGATTTTGAACCCGAAATTGGAAACGTTTTATTATGTCGAACTTATCGGAATCAGCGTCGGCGGAGTCCGAGTCCGCGGCATCTCCGCCTCCCTCTTCAAGCTCGATTCCGCCGGCAACGGCGGCGTCATAATCGATTCGGGTACATCGGTAACCCGGCTGACCCGACCCGCGTACACTGCTCTTCGCGACGCGTTCCGGGCTGGAGCGTCCCATTTAAAAAGAGGTCCCGAGTTTTCGCTGTTCGATACGTGTTACGACTTGTCGGGACAGTCGGCGGTGAAGGTCCCGACAGTGGTGCTGCATTTCCGGGGAGCTGACATGGCATTGCCGGCGACAAATTATTTGATACCAGTAGACGACAGTGGAAGCTTTTGCTTTGCGTTTGCGGGTACCATGTCCGGATTGTCGATAATTGGGAATATTCAACAGCAAGGGTTTCGGGTCGTGTACGATTTGGCGGGTTCTCGGATCGGGTTCGCTCCACGTGGGTGCACGTGA

mRNA sequence

ATGGAGTCTCCTCCAAGATATCTCCTCTTCTTCTTCTTCTTCTTCTTCGCCGCCGTGGCCTCCGCCGCGTCGGAGTTCCAAACCCTTACTCTCCGCCGTCTTCCAATTCCCTCTCCCCTTCCCTTTCCACAACCCCAATTCGACACCCAAGAAACGCTCGAATCCACCGCCGCCCTCACGGTTGAGCTCCACCATTTGGATTCACTCTCTACCAACAAAACCCCCTCCGATCTCTTCAACCTCCGGCTCCACCGCGACGCCCTCCGTGTTGACTCGTTGACCTCCCTGACTGCTGGCCGGAGCCGGACTCCTCTCCGGCGAGCCGGGTTCAGTAGCTCTGTTATCTCCGGCCTTGCTCAAGGTAGCGGTGAGTACTTCACCCGCCTTGGCGTCGGAACGCCTCCTAGATATATCTTTATGGTCCTCGACACCGGAAGCGACGTCGTTTGGCTTCAATGCTCCCCTTGCCGGAAATGCTACTCCCAATCTGATCCCATTTTTAACCCCTTCAAATCCAAATCCTTCGCCGGAATCCCCTGTTCTTCACCTCTCTGCCGCCGCCTTGACTCCTCCGGCTGCAACACTCGCCGCCACACCTGCCTCTACCAAGTCTCCTACGGCGATGGGTCCTTTACCACCGGCGATTTCGCCACCGAAACGCTCACGTTTCGTGGGAATAAAATCGCCAAAGTCGCCCTCGGCTGCGGCCACGACAATGAAGGCCTCTTCGTCGGTGCCGCTGGATTATTGGGTCTTGGCCGTGGTCGGTTTTCTTTCCCTTCTCAAACCGGACTCCGGTTCAACCATAAATTTTCTTATTGTTTGGTGGACCGGTCCGCTTCCTCCAAACCCTCCTCTATGGTTTTCGGTGATGCGGCAATTTCCCGGCTCGCCCGGTTCACTCCTCTGATTTTGAACCCGAAATTGGAAACGTTTTATTATGTCGAACTTATCGGAATCAGCGTCGGCGGAGTCCGAGTCCGCGGCATCTCCGCCTCCCTCTTCAAGCTCGATTCCGCCGGCAACGGCGGCGTCATAATCGATTCGGGTACATCGGTAACCCGGCTGACCCGACCCGCGTACACTGCTCTTCGCGACGCGTTCCGGGCTGGAGCGTCCCATTTAAAAAGAGGTCCCGAGTTTTCGCTGTTCGATACGTGTTACGACTTGTCGGGACAGTCGGCGGTGAAGGTCCCGACAGTGGTGCTGCATTTCCGGGGAGCTGACATGGCATTGCCGGCGACAAATTATTTGATACCAGTAGACGACAGTGGAAGCTTTTGCTTTGCGTTTGCGGGTACCATGTCCGGATTGTCGATAATTGGGAATATTCAACAGCAAGGGTTTCGGGTCGTGTACGATTTGGCGGGTTCTCGGATCGGGTTCGCTCCACGTGGGTGCACGTGA

Coding sequence (CDS)

ATGGAGTCTCCTCCAAGATATCTCCTCTTCTTCTTCTTCTTCTTCTTCGCCGCCGTGGCCTCCGCCGCGTCGGAGTTCCAAACCCTTACTCTCCGCCGTCTTCCAATTCCCTCTCCCCTTCCCTTTCCACAACCCCAATTCGACACCCAAGAAACGCTCGAATCCACCGCCGCCCTCACGGTTGAGCTCCACCATTTGGATTCACTCTCTACCAACAAAACCCCCTCCGATCTCTTCAACCTCCGGCTCCACCGCGACGCCCTCCGTGTTGACTCGTTGACCTCCCTGACTGCTGGCCGGAGCCGGACTCCTCTCCGGCGAGCCGGGTTCAGTAGCTCTGTTATCTCCGGCCTTGCTCAAGGTAGCGGTGAGTACTTCACCCGCCTTGGCGTCGGAACGCCTCCTAGATATATCTTTATGGTCCTCGACACCGGAAGCGACGTCGTTTGGCTTCAATGCTCCCCTTGCCGGAAATGCTACTCCCAATCTGATCCCATTTTTAACCCCTTCAAATCCAAATCCTTCGCCGGAATCCCCTGTTCTTCACCTCTCTGCCGCCGCCTTGACTCCTCCGGCTGCAACACTCGCCGCCACACCTGCCTCTACCAAGTCTCCTACGGCGATGGGTCCTTTACCACCGGCGATTTCGCCACCGAAACGCTCACGTTTCGTGGGAATAAAATCGCCAAAGTCGCCCTCGGCTGCGGCCACGACAATGAAGGCCTCTTCGTCGGTGCCGCTGGATTATTGGGTCTTGGCCGTGGTCGGTTTTCTTTCCCTTCTCAAACCGGACTCCGGTTCAACCATAAATTTTCTTATTGTTTGGTGGACCGGTCCGCTTCCTCCAAACCCTCCTCTATGGTTTTCGGTGATGCGGCAATTTCCCGGCTCGCCCGGTTCACTCCTCTGATTTTGAACCCGAAATTGGAAACGTTTTATTATGTCGAACTTATCGGAATCAGCGTCGGCGGAGTCCGAGTCCGCGGCATCTCCGCCTCCCTCTTCAAGCTCGATTCCGCCGGCAACGGCGGCGTCATAATCGATTCGGGTACATCGGTAACCCGGCTGACCCGACCCGCGTACACTGCTCTTCGCGACGCGTTCCGGGCTGGAGCGTCCCATTTAAAAAGAGGTCCCGAGTTTTCGCTGTTCGATACGTGTTACGACTTGTCGGGACAGTCGGCGGTGAAGGTCCCGACAGTGGTGCTGCATTTCCGGGGAGCTGACATGGCATTGCCGGCGACAAATTATTTGATACCAGTAGACGACAGTGGAAGCTTTTGCTTTGCGTTTGCGGGTACCATGTCCGGATTGTCGATAATTGGGAATATTCAACAGCAAGGGTTTCGGGTCGTGTACGATTTGGCGGGTTCTCGGATCGGGTTCGCTCCACGTGGGTGCACGTGA

Protein sequence

MESPPRYLLFFFFFFFAAVASAASEFQTLTLRRLPIPSPLPFPQPQFDTQETLESTAALTVELHHLDSLSTNKTPSDLFNLRLHRDALRVDSLTSLTAGRSRTPLRRAGFSSSVISGLAQGSGEYFTRLGVGTPPRYIFMVLDTGSDVVWLQCSPCRKCYSQSDPIFNPFKSKSFAGIPCSSPLCRRLDSSGCNTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDNEGLFVGAAGLLGLGRGRFSFPSQTGLRFNHKFSYCLVDRSASSKPSSMVFGDAAISRLARFTPLILNPKLETFYYVELIGISVGGVRVRGISASLFKLDSAGNGGVIIDSGTSVTRLTRPAYTALRDAFRAGASHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMALPATNYLIPVDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT
Homology
BLAST of Carg04941 vs. NCBI nr
Match: KAG7025313.1 (Aspartyl protease family protein 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 938.7 bits (2425), Expect = 1.9e-269
Identity = 468/468 (100.00%), Postives = 468/468 (100.00%), Query Frame = 0

Query: 1   MESPPRYLLFFFFFFFAAVASAASEFQTLTLRRLPIPSPLPFPQPQFDTQETLESTAALT 60
           MESPPRYLLFFFFFFFAAVASAASEFQTLTLRRLPIPSPLPFPQPQFDTQETLESTAALT
Sbjct: 1   MESPPRYLLFFFFFFFAAVASAASEFQTLTLRRLPIPSPLPFPQPQFDTQETLESTAALT 60

Query: 61  VELHHLDSLSTNKTPSDLFNLRLHRDALRVDSLTSLTAGRSRTPLRRAGFSSSVISGLAQ 120
           VELHHLDSLSTNKTPSDLFNLRLHRDALRVDSLTSLTAGRSRTPLRRAGFSSSVISGLAQ
Sbjct: 61  VELHHLDSLSTNKTPSDLFNLRLHRDALRVDSLTSLTAGRSRTPLRRAGFSSSVISGLAQ 120

Query: 121 GSGEYFTRLGVGTPPRYIFMVLDTGSDVVWLQCSPCRKCYSQSDPIFNPFKSKSFAGIPC 180
           GSGEYFTRLGVGTPPRYIFMVLDTGSDVVWLQCSPCRKCYSQSDPIFNPFKSKSFAGIPC
Sbjct: 121 GSGEYFTRLGVGTPPRYIFMVLDTGSDVVWLQCSPCRKCYSQSDPIFNPFKSKSFAGIPC 180

Query: 181 SSPLCRRLDSSGCNTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDNE 240
           SSPLCRRLDSSGCNTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDNE
Sbjct: 181 SSPLCRRLDSSGCNTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDNE 240

Query: 241 GLFVGAAGLLGLGRGRFSFPSQTGLRFNHKFSYCLVDRSASSKPSSMVFGDAAISRLARF 300
           GLFVGAAGLLGLGRGRFSFPSQTGLRFNHKFSYCLVDRSASSKPSSMVFGDAAISRLARF
Sbjct: 241 GLFVGAAGLLGLGRGRFSFPSQTGLRFNHKFSYCLVDRSASSKPSSMVFGDAAISRLARF 300

Query: 301 TPLILNPKLETFYYVELIGISVGGVRVRGISASLFKLDSAGNGGVIIDSGTSVTRLTRPA 360
           TPLILNPKLETFYYVELIGISVGGVRVRGISASLFKLDSAGNGGVIIDSGTSVTRLTRPA
Sbjct: 301 TPLILNPKLETFYYVELIGISVGGVRVRGISASLFKLDSAGNGGVIIDSGTSVTRLTRPA 360

Query: 361 YTALRDAFRAGASHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMALPATNYLIP 420
           YTALRDAFRAGASHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMALPATNYLIP
Sbjct: 361 YTALRDAFRAGASHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMALPATNYLIP 420

Query: 421 VDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT 469
           VDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT
Sbjct: 421 VDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT 468

BLAST of Carg04941 vs. NCBI nr
Match: XP_022959948.1 (aspartyl protease family protein 2 [Cucurbita moschata])

HSP 1 Score: 929.9 bits (2402), Expect = 8.8e-267
Identity = 464/468 (99.15%), Postives = 465/468 (99.36%), Query Frame = 0

Query: 1   MESPPRYLLFFFFFFFAAVASAASEFQTLTLRRLPIPSPLPFPQPQFDTQETLESTAALT 60
           MESPPRYLLFFFFFFFAAVASAASEFQTLTLRRLPIPSPLPFPQPQFDTQETLESTAALT
Sbjct: 1   MESPPRYLLFFFFFFFAAVASAASEFQTLTLRRLPIPSPLPFPQPQFDTQETLESTAALT 60

Query: 61  VELHHLDSLSTNKTPSDLFNLRLHRDALRVDSLTSLTAGRSRTPLRRAGFSSSVISGLAQ 120
           VELHHLDSLS NKTPSDLFNLRLHRDALRVDSLTSLTA RSRTPLRRAGFSSSVISGLAQ
Sbjct: 61  VELHHLDSLSPNKTPSDLFNLRLHRDALRVDSLTSLTAARSRTPLRRAGFSSSVISGLAQ 120

Query: 121 GSGEYFTRLGVGTPPRYIFMVLDTGSDVVWLQCSPCRKCYSQSDPIFNPFKSKSFAGIPC 180
           GSGEYFTRLGVGTP RYI+MVLDTGSDVVWLQCSPCRKCYSQSDPIFNPFKSKSFAGIPC
Sbjct: 121 GSGEYFTRLGVGTPSRYIYMVLDTGSDVVWLQCSPCRKCYSQSDPIFNPFKSKSFAGIPC 180

Query: 181 SSPLCRRLDSSGCNTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDNE 240
           SSPLCRRLDSSGCNTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDNE
Sbjct: 181 SSPLCRRLDSSGCNTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDNE 240

Query: 241 GLFVGAAGLLGLGRGRFSFPSQTGLRFNHKFSYCLVDRSASSKPSSMVFGDAAISRLARF 300
           GLFVGAAGLLGLGRGRFSFPSQTGLRFNHKFSYCLVDRSASSKPSSMVFGDAAISRLARF
Sbjct: 241 GLFVGAAGLLGLGRGRFSFPSQTGLRFNHKFSYCLVDRSASSKPSSMVFGDAAISRLARF 300

Query: 301 TPLILNPKLETFYYVELIGISVGGVRVRGISASLFKLDSAGNGGVIIDSGTSVTRLTRPA 360
           TPLILNPKLETFYYVELIGISVGGVRVRGISASLFKLDSAGNGGVIIDSGTSVTRLTRPA
Sbjct: 301 TPLILNPKLETFYYVELIGISVGGVRVRGISASLFKLDSAGNGGVIIDSGTSVTRLTRPA 360

Query: 361 YTALRDAFRAGASHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMALPATNYLIP 420
           YTALRDAFRAGASHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMALPATNYLIP
Sbjct: 361 YTALRDAFRAGASHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMALPATNYLIP 420

Query: 421 VDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT 469
           VDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT
Sbjct: 421 VDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT 468

BLAST of Carg04941 vs. NCBI nr
Match: XP_023514169.1 (aspartyl protease family protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 924.9 bits (2389), Expect = 2.8e-265
Identity = 463/468 (98.93%), Postives = 464/468 (99.15%), Query Frame = 0

Query: 1   MESPPRYLLFFFFFFFAAVASAASEFQTLTLRRLPIPSPLPFPQPQFDTQETLESTAALT 60
           MESPPRYLL FFFFFFAAVASAASEFQTLTLRRLPIPSPLPFPQPQFDTQETLESTAALT
Sbjct: 1   MESPPRYLL-FFFFFFAAVASAASEFQTLTLRRLPIPSPLPFPQPQFDTQETLESTAALT 60

Query: 61  VELHHLDSLSTNKTPSDLFNLRLHRDALRVDSLTSLTAGRSRTPLRRAGFSSSVISGLAQ 120
           VELHHLDSLS NKTPSDLFNLRLHRDALRVDSLTSLTA RSRTPLRRAGFSSSVISGLAQ
Sbjct: 61  VELHHLDSLSPNKTPSDLFNLRLHRDALRVDSLTSLTAARSRTPLRRAGFSSSVISGLAQ 120

Query: 121 GSGEYFTRLGVGTPPRYIFMVLDTGSDVVWLQCSPCRKCYSQSDPIFNPFKSKSFAGIPC 180
           GSGEYFTRLGVGTPPRYI+MVLDTGSDVVWLQCSPCRKCYSQSDPIFNPFKSKSFAGIPC
Sbjct: 121 GSGEYFTRLGVGTPPRYIYMVLDTGSDVVWLQCSPCRKCYSQSDPIFNPFKSKSFAGIPC 180

Query: 181 SSPLCRRLDSSGCNTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDNE 240
           SSPLCRRLDSSGCNTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDNE
Sbjct: 181 SSPLCRRLDSSGCNTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDNE 240

Query: 241 GLFVGAAGLLGLGRGRFSFPSQTGLRFNHKFSYCLVDRSASSKPSSMVFGDAAISRLARF 300
           GLFVGAAGLLGLGRGRFSFPSQTGLRFNHKFSYCLVDRSASSKPSSMVFGDAAISRLARF
Sbjct: 241 GLFVGAAGLLGLGRGRFSFPSQTGLRFNHKFSYCLVDRSASSKPSSMVFGDAAISRLARF 300

Query: 301 TPLILNPKLETFYYVELIGISVGGVRVRGISASLFKLDSAGNGGVIIDSGTSVTRLTRPA 360
           TPLILNPKLETFYYVELIGISVGGVRVRGISASLFKLDSAGNGGVIIDSGTSVTRLTRPA
Sbjct: 301 TPLILNPKLETFYYVELIGISVGGVRVRGISASLFKLDSAGNGGVIIDSGTSVTRLTRPA 360

Query: 361 YTALRDAFRAGASHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMALPATNYLIP 420
           YTALRDAFR GASHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMALPATNYLIP
Sbjct: 361 YTALRDAFRVGASHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMALPATNYLIP 420

Query: 421 VDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT 469
           VDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT
Sbjct: 421 VDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT 467

BLAST of Carg04941 vs. NCBI nr
Match: XP_023005015.1 (aspartyl protease family protein 2 [Cucurbita maxima])

HSP 1 Score: 914.1 bits (2361), Expect = 5.0e-262
Identity = 459/468 (98.08%), Postives = 461/468 (98.50%), Query Frame = 0

Query: 1   MESPPRYLLFFFFFFFAAVASAASEFQTLTLRRLPIPSPLPFPQPQFDTQETLESTAALT 60
           MESPPR LL FFFFF AAVASAASEFQTLTLRRLPIPSPL FPQ QFDTQETLESTAALT
Sbjct: 1   MESPPRNLL-FFFFFXAAVASAASEFQTLTLRRLPIPSPLSFPQSQFDTQETLESTAALT 60

Query: 61  VELHHLDSLSTNKTPSDLFNLRLHRDALRVDSLTSLTAGRSRTPLRRAGFSSSVISGLAQ 120
           VELHHLDSLSTNKTPSDLFNLRLHRDALRVDSLTSLTA RSRTPLRRAGFSSSVISGLAQ
Sbjct: 61  VELHHLDSLSTNKTPSDLFNLRLHRDALRVDSLTSLTAARSRTPLRRAGFSSSVISGLAQ 120

Query: 121 GSGEYFTRLGVGTPPRYIFMVLDTGSDVVWLQCSPCRKCYSQSDPIFNPFKSKSFAGIPC 180
           GSGEYFTRLGVGTPPRY++MVLDTGSDVVWLQCSPCRKCYSQSDPIFNPFKSKSFAGIPC
Sbjct: 121 GSGEYFTRLGVGTPPRYLYMVLDTGSDVVWLQCSPCRKCYSQSDPIFNPFKSKSFAGIPC 180

Query: 181 SSPLCRRLDSSGCNTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDNE 240
           SSPLCRRLDSSGCNTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDNE
Sbjct: 181 SSPLCRRLDSSGCNTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDNE 240

Query: 241 GLFVGAAGLLGLGRGRFSFPSQTGLRFNHKFSYCLVDRSASSKPSSMVFGDAAISRLARF 300
           GLFVGAAGLLGLGRGRFSFPSQTGLRFNHKFSYCLVDRSASSKPSSMVFGDAAISRLARF
Sbjct: 241 GLFVGAAGLLGLGRGRFSFPSQTGLRFNHKFSYCLVDRSASSKPSSMVFGDAAISRLARF 300

Query: 301 TPLILNPKLETFYYVELIGISVGGVRVRGISASLFKLDSAGNGGVIIDSGTSVTRLTRPA 360
           TPLILNPKLETFYYVELIG SVGGVRVRGISASLFKLDSAGNGGVIIDSGTSVTRLTRPA
Sbjct: 301 TPLILNPKLETFYYVELIGFSVGGVRVRGISASLFKLDSAGNGGVIIDSGTSVTRLTRPA 360

Query: 361 YTALRDAFRAGASHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMALPATNYLIP 420
           YTALRDAFRAGASHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMALPATNYLIP
Sbjct: 361 YTALRDAFRAGASHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMALPATNYLIP 420

Query: 421 VDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT 469
           VDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT
Sbjct: 421 VDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT 467

BLAST of Carg04941 vs. NCBI nr
Match: KAG6592908.1 (Aspartyl protease family protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 859.0 bits (2218), Expect = 1.9e-245
Identity = 430/448 (95.98%), Postives = 433/448 (96.65%), Query Frame = 0

Query: 31  LRRLPIPSPL----------PFPQPQFDTQETLESTAALTVELHHLDSLSTNKTPSDLFN 90
           LRR+ +P+P             PQPQFDTQETLESTAALTVELHHLDSLSTNKTPSDLFN
Sbjct: 61  LRRVGVPNPYSPPSSNSLSPSLPQPQFDTQETLESTAALTVELHHLDSLSTNKTPSDLFN 120

Query: 91  LRLHRDALRVDSLTSLTAGRSRTPLRRAGFSSSVISGLAQGSGEYFTRLGVGTPPRYIFM 150
           LRLHRDALRVDSLTSLTA RSRTPLRRAGFSSSVISGLAQGSGEYFTRLGVGTPPRYIFM
Sbjct: 121 LRLHRDALRVDSLTSLTAARSRTPLRRAGFSSSVISGLAQGSGEYFTRLGVGTPPRYIFM 180

Query: 151 VLDTGSDVVWLQCSPCRKCYSQSDPIFNPFKSKSFAGIPCSSPLCRRLDSSGCNTRRHTC 210
           VLDTGSDVVWLQCSPCRKCYSQSDPIFNPFKSKSFAGIPCSSPLCRRLDSSGCNTRRHTC
Sbjct: 181 VLDTGSDVVWLQCSPCRKCYSQSDPIFNPFKSKSFAGIPCSSPLCRRLDSSGCNTRRHTC 240

Query: 211 LYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDNEGLFVGAAGLLGLGRGRFSFP 270
           LYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDNEGLFVGAAGLLGLGRGRFSFP
Sbjct: 241 LYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDNEGLFVGAAGLLGLGRGRFSFP 300

Query: 271 SQTGLRFNHKFSYCLVDRSASSKPSSMVFGDAAISRLARFTPLILNPKLETFYYVELIGI 330
           SQTGLRFNHKFSYCLVDRSASSKPSSMVFGDAAISRLARFTPLILNPKLETFYYVELIGI
Sbjct: 301 SQTGLRFNHKFSYCLVDRSASSKPSSMVFGDAAISRLARFTPLILNPKLETFYYVELIGI 360

Query: 331 SVGGVRVRGISASLFKLDSAGNGGVIIDSGTSVTRLTRPAYTALRDAFRAGASHLKRGPE 390
           SVGGVRVRGISASLFKLDSAGNGGVIIDSGTSVTRLTRPAYTALRDAFRAGASHLKRGPE
Sbjct: 361 SVGGVRVRGISASLFKLDSAGNGGVIIDSGTSVTRLTRPAYTALRDAFRAGASHLKRGPE 420

Query: 391 FSLFDTCYDLSGQSAVKVPTVVLHFRGADMALPATNYLIPVDDSGSFCFAFAGTMSGLSI 450
           FSLFDTCYDLSGQSAVKVPTVVLHFRGADMALPATNYLIPVDDSGSFCFAFAGTMSGLSI
Sbjct: 421 FSLFDTCYDLSGQSAVKVPTVVLHFRGADMALPATNYLIPVDDSGSFCFAFAGTMSGLSI 480

Query: 451 IGNIQQQGFRVVYDLAGSRIGFAPRGCT 469
           IGNIQQQGFRVVYDLAGSRIGFAPRGCT
Sbjct: 481 IGNIQQQGFRVVYDLAGSRIGFAPRGCT 508

BLAST of Carg04941 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 671.8 bits (1732), Expect = 5.7e-192
Identity = 341/478 (71.34%), Postives = 394/478 (82.43%), Query Frame = 0

Query: 8   LLFFFFFFFAAVASAAS--EFQTL--TLRRLPIPSPLPFPQPQFDTQETL---------- 67
           LLF   FFF ++ S +S   FQTL      LP  SP+ F QP  D++  L          
Sbjct: 8   LLFSLCFFFLSLPSFSSLPSFQTLFPNSHSLPCASPVSF-QPDSDSESLLESEFESGSDS 67

Query: 68  ESTAALTVELHHLDSLSTNKTPSDLFNLRLHRDALRVDSLTSLTA---GRSRTPL-RRAG 127
           ES++++T+ L H+D+LS+NKTP +LF+ RL RD+ RV S+ +L A   GR+ T   R  G
Sbjct: 68  ESSSSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAPRPGG 127

Query: 128 FSSSVISGLAQGSGEYFTRLGVGTPPRYIFMVLDTGSDVVWLQCSPCRKCYSQSDPIFNP 187
           FSSSV+SGL+QGSGEYFTRLGVGTP RY++MVLDTGSD+VWLQC+PCR+CYSQSDPIF+P
Sbjct: 128 FSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFDP 187

Query: 188 FKSKSFAGIPCSSPLCRRLDSSGCNTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIA 247
            KSK++A IPCSSP CRRLDS+GCNTRR TCLYQVSYGDGSFT GDF+TETLTFR N++ 
Sbjct: 188 RKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRRNRVK 247

Query: 248 KVALGCGHDNEGLFVGAAGLLGLGRGRFSFPSQTGLRFNHKFSYCLVDRSASSKPSSMVF 307
            VALGCGHDNEGLFVGAAGLLGLG+G+ SFP QTG RFN KFSYCLVDRSASSKPSS+VF
Sbjct: 248 GVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVF 307

Query: 308 GDAAISRLARFTPLILNPKLETFYYVELIGISVGGVRVRGISASLFKLDSAGNGGVIIDS 367
           G+AA+SR+ARFTPL+ NPKL+TFYYV L+GISVGG RV G++ASLFKLD  GNGGVIIDS
Sbjct: 308 GNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDS 367

Query: 368 GTSVTRLTRPAYTALRDAFRAGASHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGAD 427
           GTSVTRL RPAY A+RDAFR GA  LKR P+FSLFDTC+DLS  + VKVPTVVLHFRGAD
Sbjct: 368 GTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHFRGAD 427

Query: 428 MALPATNYLIPVDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGC 468
           ++LPATNYLIPVD +G FCFAFAGTM GLSIIGNIQQQGFRVVYDLA SR+GFAP GC
Sbjct: 428 VSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGC 484

BLAST of Carg04941 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 400.2 bits (1027), Expect = 3.2e-110
Identity = 230/469 (49.04%), Postives = 294/469 (62.69%), Query Frame = 0

Query: 19  VASAASEFQTLTLRRLPIPSPLPFPQPQ-FDTQETLESTAALTVELHHLDSL--STNKTP 78
           V S+  + QT+ L   P  S L   +P+         S++ L++ELH  D+   S +K  
Sbjct: 40  VVSSLQQTQTI-LSLDPTRSSLTTTKPESLSDPVFFNSSSPLSLELHSRDTFVASQHKDY 99

Query: 79  SDLFNLRLHRDALRVDSLTS----LTAGRSRTPLR----------RAGFSSSVISGLAQG 138
             L   RL RD+ RV  + +       G  R+ L+              ++ V+SG +QG
Sbjct: 100 KSLTLSRLERDSSRVAGIVAKIRFAVEGVDRSDLKPVYNEDTRYQTEDLTTPVVSGASQG 159

Query: 139 SGEYFTRLGVGTPPRYIFMVLDTGSDVVWLQCSPCRKCYSQSDPIFNPFKSKSFAGIPCS 198
           SGEYF+R+GVGTP + +++VLDTGSDV W+QC PC  CY QSDP+FNP  S ++  + CS
Sbjct: 160 SGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVFNPTSSSTYKSLTCS 219

Query: 199 SPLCRRLDSSGCNTRRHTCLYQVSYGDGSFTTGDFATETLTF-RGNKIAKVALGCGHDNE 258
           +P C  L++S C  R + CLYQVSYGDGSFT G+ AT+T+TF    KI  VALGCGHDNE
Sbjct: 220 APQCSLLETSAC--RSNKCLYQVSYGDGSFTVGELATDTVTFGNSGKINNVALGCGHDNE 279

Query: 259 GLFVGAAGLLGLGRGRFSFPSQTGLRFNHKFSYCLVDRSASSKPSSMVFGDAAISRLARF 318
           GLF GAAGLLGLG G  S  +Q        FSYCLVDR  S K SS+ F    +      
Sbjct: 280 GLFTGAAGLLGLGGGVLSITNQMKAT---SFSYCLVDRD-SGKSSSLDFNSVQLGGGDAT 339

Query: 319 TPLILNPKLETFYYVELIGISVGGVRVRGISASLFKLDSAGNGGVIIDSGTSVTRLTRPA 378
            PL+ N K++TFYYV L G SVGG +V  +  ++F +D++G+GGVI+D GT+VTRL   A
Sbjct: 340 APLLRNKKIDTFYYVGLSGFSVGGEKV-VLPDAIFDVDASGSGGVILDCGTAVTRLQTQA 399

Query: 379 YTALRDAFRAGASHLKRG-PEFSLFDTCYDLSGQSAVKVPTVVLHFRGA-DMALPATNYL 438
           Y +LRDAF     +LK+G    SLFDTCYD S  S VKVPTV  HF G   + LPA NYL
Sbjct: 400 YNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLSTVKVPTVAFHFTGGKSLDLPAKNYL 459

Query: 439 IPVDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGC 468
           IPVDDSG+FCFAFA T S LSIIGN+QQQG R+ YDL+ + IG +   C
Sbjct: 460 IPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

BLAST of Carg04941 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 5.1e-108
Identity = 211/471 (44.80%), Postives = 296/471 (62.85%), Query Frame = 0

Query: 8   LLFFFFFFFAAVASAAS----EFQTLTLRRLPIPSPLPFPQPQFDTQETLESTAALTVEL 67
           L FFF      ++S++S    +FQ + + + P+      P    +T  + ES++  T+ L
Sbjct: 5   LFFFFLHLHLHLSSSSSISFPDFQIIDVLQPPLTVTATLPDFN-NTHFSDESSSKYTLRL 64

Query: 68  HHLDSLS--TNKTPSDLFNLRLHRDALRVDSLTSLTAGR----SRTPLRRAGFSSSVISG 127
            H D     T +      + R+ RD  RV ++    +G+    S +      F S ++SG
Sbjct: 65  LHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKVIPSSDSRYEVNDFGSDIVSG 124

Query: 128 LAQGSGEYFTRLGVGTPPRYIFMVLDTGSDVVWLQCSPCRKCYSQSDPIFNPFKSKSFAG 187
           + QGSGEYF R+GVG+PPR  +MV+D+GSD+VW+QC PC+ CY QSDP+F+P KS S+ G
Sbjct: 125 MDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSDPVFDPAKSGSYTG 184

Query: 188 IPCSSPLCRRLDSSGCNTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGH 247
           + C S +C R+++SGC++    C Y+V YGDGS+T G  A ETLTF    +  VA+GCGH
Sbjct: 185 VSCGSSVCDRIENSGCHS--GGCRYEVMYGDGSYTKGTLALETLTFAKTVVRNVAMGCGH 244

Query: 248 DNEGLFVGAAGLLGLGRGRFSFPSQTGLRFNHKFSYCLVDRSASSKPSSMVFGDAAISRL 307
            N G+F+GAAGLLG+G G  SF  Q   +    F YCLV R   S   S+VFG  A+   
Sbjct: 245 RNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDS-TGSLVFGREALPVG 304

Query: 308 ARFTPLILNPKLETFYYVELIGISVGGVRVRGISASLFKLDSAGNGGVIIDSGTSVTRLT 367
           A + PL+ NP+  +FYYV L G+ VGGVR+  +   +F L   G+GGV++D+GT+VTRL 
Sbjct: 305 ASWVPLVRNPRAPSFYYVGLKGLGVGGVRI-PLPDGVFDLTETGDGGVVMDTGTAVTRLP 364

Query: 368 RPAYTALRDAFRAGASHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHF-RGADMALPATN 427
             AY A RD F++  ++L R    S+FDTCYDLSG  +V+VPTV  +F  G  + LPA N
Sbjct: 365 TAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPVLTLPARN 424

Query: 428 YLIPVDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGC 468
           +L+PVDDSG++CFAFA + +GLSIIGNIQQ+G +V +D A   +GF P  C
Sbjct: 425 FLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

BLAST of Carg04941 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 1.4e-73
Identity = 169/412 (41.02%), Postives = 227/412 (55.10%), Query Frame = 0

Query: 61  VELHHLDSLSTNKTPSDLFNLRLHRDALRVDSLTSLTAGRSRTPLRRAGFSSSVISGLAQ 120
           + L H+DS   N T   L    + R + R+  L ++  G S       G  +SV +    
Sbjct: 43  IMLEHVDS-GKNLTKFQLLERAIERGSRRLQRLEAMLNGPS-------GVETSVYA---- 102

Query: 121 GSGEYFTRLGVGTPPRYIFMVLDTGSDVVWLQCSPCRKCYSQSDPIFNPFKSKSFAGIPC 180
           G GEY   L +GTP +    ++DTGSD++W QC PC +C++QS PIFNP  S SF+ +PC
Sbjct: 103 GDGEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPC 162

Query: 181 SSPLCRRLDSSGCNTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDNE 240
           SS LC+ L S  C+   + C Y   YGDGS T G   TETLTF    I  +  GCG +N+
Sbjct: 163 SSQLCQALSSPTCS--NNFCQYTYGYGDGSETQGSMGTETLTFGSVSIPNITFGCGENNQ 222

Query: 241 GLFVG-AAGLLGLGRGRFSFPSQTGLRFNHKFSYCLVDRSASSKPSSMVFGDAAISRLAR 300
           G   G  AGL+G+GRG  S PSQ  +    KFSYC+     SS PS+++ G  A S  A 
Sbjct: 223 GFGQGNGAGLVGMGRGPLSLPSQLDVT---KFSYCMTP-IGSSTPSNLLLGSLANSVTAG 282

Query: 301 F--TPLILNPKLETFYYVELIGISVGGVRVRGISASLFKLDS-AGNGGVIIDSGTSVTRL 360
              T LI + ++ TFYY+ L G+SVG  R+  I  S F L+S  G GG+IIDSGT++T  
Sbjct: 283 SPNTTLIQSSQIPTFYYITLNGLSVGSTRL-PIDPSAFALNSNNGTGGIIIDSGTTLTYF 342

Query: 361 TRPAYTALRDAFRAGASHLKRGPEFSLFDTCYDL-SGQSAVKVPTVVLHFRGADMALPAT 420
              AY ++R  F +  +        S FD C+   S  S +++PT V+HF G D+ LP+ 
Sbjct: 343 VNNAYQSVRQEFISQINLPVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFDGGDLELPSE 402

Query: 421 NYLIPVDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGC 468
           NY I    +G  C A   +  G+SI GNIQQQ   VVYD   S + FA   C
Sbjct: 403 NYFIS-PSNGLICLAMGSSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

BLAST of Carg04941 vs. ExPASy Swiss-Prot
Match: Q8S9J6 (Aspartyl protease family protein At5g10770 OS=Arabidopsis thaliana OX=3702 GN=At5g10770 PE=2 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 1.8e-73
Identity = 161/395 (40.76%), Postives = 216/395 (54.68%), Query Frame = 0

Query: 83  LHRDALRVDSLTSLTAGRSRTPLRRAGFSSSVIS--GLAQGSGEYFTRLGVGTPPRYIFM 142
           L  D  RV+S+ S  + +  T       S+ + +  G   GSG Y   +G+GTP   + +
Sbjct: 88  LRLDQARVNSIHSKLSKKLATDHVSESKSTDLPAKDGSTLGSGNYIVTVGLGTPKNDLSL 147

Query: 143 VLDTGSDVVWLQCSPC-RKCYSQSDPIFNPFKSKSFAGIPCSSPLCRRLDSSGCNT---R 202
           + DTGSD+ W QC PC R CY Q +PIFNP KS S+  + CSS  C  L S+  N     
Sbjct: 148 IFDTGSDLTWTQCQPCVRTCYDQKEPIFNPSKSTSYYNVSCSSAACGSLSSATGNAGSCS 207

Query: 203 RHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAK-VALGCGHDNEGLFVGAAGLLGLGRG 262
              C+Y + YGD SF+ G  A E  T   + +   V  GCG +N+GLF G AGLLGLGR 
Sbjct: 208 ASNCIYGIQYGDQSFSVGFLAKEKFTLTNSDVFDGVYFGCGENNQGLFTGVAGLLGLGRD 267

Query: 263 RFSFPSQTGLRFNHKFSYCLVDRSASSKPSSMVFGDAAISRLARFTPLILNPKLETFYYV 322
           + SFPSQT   +N  FSYCL   S++S    + FG A ISR  +FTP+       +FY +
Sbjct: 268 KLSFPSQTATAYNKIFSYCL--PSSASYTGHLTFGSAGISRSVKFTPISTITDGTSFYGL 327

Query: 323 ELIGISVGGVRVRGISASLFKLDSAGNGGVIIDSGTSVTRLTRPAYTALRDAFRAGASHL 382
            ++ I+VGG ++  I +++F        G +IDSGT +TRL   AY ALR +F+A  S  
Sbjct: 328 NIVAITVGGQKL-PIPSTVF-----STPGALIDSGTVITRLPPKAYAALRSSFKAKMSKY 387

Query: 383 KRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMALPATNYLIPVDDSGSFCFAFAGTM 442
                 S+ DTC+DLSG   V +P V   F G  +    +  +  V      C AFAG  
Sbjct: 388 PTTSGVSILDTCFDLSGFKTVTIPKVAFSFSGGAVVELGSKGIFYVFKISQVCLAFAGNS 447

Query: 443 --SGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT 469
             S  +I GN+QQQ   VVYD AG R+GFAP GC+
Sbjct: 448 DDSNAAIFGNVQQQTLEVVYDGAGGRVGFAPNGCS 474

BLAST of Carg04941 vs. ExPASy TrEMBL
Match: A0A6J1H7E0 (aspartyl protease family protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111460848 PE=3 SV=1)

HSP 1 Score: 929.9 bits (2402), Expect = 4.3e-267
Identity = 464/468 (99.15%), Postives = 465/468 (99.36%), Query Frame = 0

Query: 1   MESPPRYLLFFFFFFFAAVASAASEFQTLTLRRLPIPSPLPFPQPQFDTQETLESTAALT 60
           MESPPRYLLFFFFFFFAAVASAASEFQTLTLRRLPIPSPLPFPQPQFDTQETLESTAALT
Sbjct: 1   MESPPRYLLFFFFFFFAAVASAASEFQTLTLRRLPIPSPLPFPQPQFDTQETLESTAALT 60

Query: 61  VELHHLDSLSTNKTPSDLFNLRLHRDALRVDSLTSLTAGRSRTPLRRAGFSSSVISGLAQ 120
           VELHHLDSLS NKTPSDLFNLRLHRDALRVDSLTSLTA RSRTPLRRAGFSSSVISGLAQ
Sbjct: 61  VELHHLDSLSPNKTPSDLFNLRLHRDALRVDSLTSLTAARSRTPLRRAGFSSSVISGLAQ 120

Query: 121 GSGEYFTRLGVGTPPRYIFMVLDTGSDVVWLQCSPCRKCYSQSDPIFNPFKSKSFAGIPC 180
           GSGEYFTRLGVGTP RYI+MVLDTGSDVVWLQCSPCRKCYSQSDPIFNPFKSKSFAGIPC
Sbjct: 121 GSGEYFTRLGVGTPSRYIYMVLDTGSDVVWLQCSPCRKCYSQSDPIFNPFKSKSFAGIPC 180

Query: 181 SSPLCRRLDSSGCNTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDNE 240
           SSPLCRRLDSSGCNTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDNE
Sbjct: 181 SSPLCRRLDSSGCNTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDNE 240

Query: 241 GLFVGAAGLLGLGRGRFSFPSQTGLRFNHKFSYCLVDRSASSKPSSMVFGDAAISRLARF 300
           GLFVGAAGLLGLGRGRFSFPSQTGLRFNHKFSYCLVDRSASSKPSSMVFGDAAISRLARF
Sbjct: 241 GLFVGAAGLLGLGRGRFSFPSQTGLRFNHKFSYCLVDRSASSKPSSMVFGDAAISRLARF 300

Query: 301 TPLILNPKLETFYYVELIGISVGGVRVRGISASLFKLDSAGNGGVIIDSGTSVTRLTRPA 360
           TPLILNPKLETFYYVELIGISVGGVRVRGISASLFKLDSAGNGGVIIDSGTSVTRLTRPA
Sbjct: 301 TPLILNPKLETFYYVELIGISVGGVRVRGISASLFKLDSAGNGGVIIDSGTSVTRLTRPA 360

Query: 361 YTALRDAFRAGASHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMALPATNYLIP 420
           YTALRDAFRAGASHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMALPATNYLIP
Sbjct: 361 YTALRDAFRAGASHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMALPATNYLIP 420

Query: 421 VDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT 469
           VDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT
Sbjct: 421 VDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT 468

BLAST of Carg04941 vs. ExPASy TrEMBL
Match: A0A6J1KW81 (aspartyl protease family protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111498134 PE=3 SV=1)

HSP 1 Score: 914.1 bits (2361), Expect = 2.4e-262
Identity = 459/468 (98.08%), Postives = 461/468 (98.50%), Query Frame = 0

Query: 1   MESPPRYLLFFFFFFFAAVASAASEFQTLTLRRLPIPSPLPFPQPQFDTQETLESTAALT 60
           MESPPR LL FFFFF AAVASAASEFQTLTLRRLPIPSPL FPQ QFDTQETLESTAALT
Sbjct: 1   MESPPRNLL-FFFFFXAAVASAASEFQTLTLRRLPIPSPLSFPQSQFDTQETLESTAALT 60

Query: 61  VELHHLDSLSTNKTPSDLFNLRLHRDALRVDSLTSLTAGRSRTPLRRAGFSSSVISGLAQ 120
           VELHHLDSLSTNKTPSDLFNLRLHRDALRVDSLTSLTA RSRTPLRRAGFSSSVISGLAQ
Sbjct: 61  VELHHLDSLSTNKTPSDLFNLRLHRDALRVDSLTSLTAARSRTPLRRAGFSSSVISGLAQ 120

Query: 121 GSGEYFTRLGVGTPPRYIFMVLDTGSDVVWLQCSPCRKCYSQSDPIFNPFKSKSFAGIPC 180
           GSGEYFTRLGVGTPPRY++MVLDTGSDVVWLQCSPCRKCYSQSDPIFNPFKSKSFAGIPC
Sbjct: 121 GSGEYFTRLGVGTPPRYLYMVLDTGSDVVWLQCSPCRKCYSQSDPIFNPFKSKSFAGIPC 180

Query: 181 SSPLCRRLDSSGCNTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDNE 240
           SSPLCRRLDSSGCNTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDNE
Sbjct: 181 SSPLCRRLDSSGCNTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDNE 240

Query: 241 GLFVGAAGLLGLGRGRFSFPSQTGLRFNHKFSYCLVDRSASSKPSSMVFGDAAISRLARF 300
           GLFVGAAGLLGLGRGRFSFPSQTGLRFNHKFSYCLVDRSASSKPSSMVFGDAAISRLARF
Sbjct: 241 GLFVGAAGLLGLGRGRFSFPSQTGLRFNHKFSYCLVDRSASSKPSSMVFGDAAISRLARF 300

Query: 301 TPLILNPKLETFYYVELIGISVGGVRVRGISASLFKLDSAGNGGVIIDSGTSVTRLTRPA 360
           TPLILNPKLETFYYVELIG SVGGVRVRGISASLFKLDSAGNGGVIIDSGTSVTRLTRPA
Sbjct: 301 TPLILNPKLETFYYVELIGFSVGGVRVRGISASLFKLDSAGNGGVIIDSGTSVTRLTRPA 360

Query: 361 YTALRDAFRAGASHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMALPATNYLIP 420
           YTALRDAFRAGASHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMALPATNYLIP
Sbjct: 361 YTALRDAFRAGASHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMALPATNYLIP 420

Query: 421 VDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT 469
           VDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT
Sbjct: 421 VDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT 467

BLAST of Carg04941 vs. ExPASy TrEMBL
Match: A0A5A7U8Z2 (Aspartyl protease family protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold673G001170 PE=3 SV=1)

HSP 1 Score: 797.0 bits (2057), Expect = 4.3e-227
Identity = 403/465 (86.67%), Postives = 425/465 (91.40%), Query Frame = 0

Query: 6   RYLLFFFFFFFAAVASAASEFQTLTLRRLPIPSPLPFPQPQFDTQETLEST--AALTVEL 65
           +YLL F+F     ++SAASEFQTLTLR LP PSPLP     F   E+L+S+  AALT++L
Sbjct: 8   KYLLLFYF-----ISSAASEFQTLTLRSLPTPSPLPL----FPDSESLQSSPAAALTLDL 67

Query: 66  HHLDSLSTNKTPSDLFNLRLHRDALRVDSLTSLTAGRSRTPLRRAGFSSSVISGLAQGSG 125
           HHLDSLS NKTP+DLFNLRLHRDALRV +LTS  A          GFSSSVISGLAQGSG
Sbjct: 68  HHLDSLSLNKTPTDLFNLRLHRDALRVHALTSRAA---------PGFSSSVISGLAQGSG 127

Query: 126 EYFTRLGVGTPPRYIFMVLDTGSDVVWLQCSPCRKCYSQSDPIFNPFKSKSFAGIPCSSP 185
           EYFTRLGVGTPPRY++MVLDTGSDVVWLQCSPCRKCYSQSDPIFNP+KSKSFAGIPCSSP
Sbjct: 128 EYFTRLGVGTPPRYLYMVLDTGSDVVWLQCSPCRKCYSQSDPIFNPYKSKSFAGIPCSSP 187

Query: 186 LCRRLDSSGCNTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDNEGLF 245
           LCRRLDSSGC+TRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGH NEGLF
Sbjct: 188 LCRRLDSSGCSTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHHNEGLF 247

Query: 246 VGAAGLLGLGRGRFSFPSQTGLRFNHKFSYCLVDRSASSKPSSMVFGDAAISRLARFTPL 305
           VGAAGLLGLGRGR SFPSQTG+RFN KFSYCLVDRSASSKPSSMVFGDAAISRLARFTPL
Sbjct: 248 VGAAGLLGLGRGRLSFPSQTGIRFNRKFSYCLVDRSASSKPSSMVFGDAAISRLARFTPL 307

Query: 306 ILNPKLETFYYVELIGISVGGVRVRGISASLFKLDSAGNGGVIIDSGTSVTRLTRPAYTA 365
           I NPKL+TFYYVELIGISVGGVRVRG+  SLFKLDSAGNGGVIIDSGTSVTRLTRPAYTA
Sbjct: 308 IRNPKLDTFYYVELIGISVGGVRVRGVYPSLFKLDSAGNGGVIIDSGTSVTRLTRPAYTA 367

Query: 366 LRDAFRAGASHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMALPATNYLIPVDD 425
           LRDAFRAGA HLKRGPEFSLFDTCYDLSGQS+VKVPTVVLHFRGADM LPATNYLIPVD+
Sbjct: 368 LRDAFRAGARHLKRGPEFSLFDTCYDLSGQSSVKVPTVVLHFRGADMPLPATNYLIPVDE 427

Query: 426 SGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT 469
           +GSFCFAFAGT+SGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT
Sbjct: 428 NGSFCFAFAGTISGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT 454

BLAST of Carg04941 vs. ExPASy TrEMBL
Match: A0A0A0K4G2 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G329350 PE=3 SV=1)

HSP 1 Score: 794.3 bits (2050), Expect = 2.8e-226
Identity = 399/465 (85.81%), Postives = 424/465 (91.18%), Query Frame = 0

Query: 6   RYLLFFFFFFFAAVASAASEFQTLTLRRLPIPSPLPFPQPQFDTQETLEST--AALTVEL 65
           +YLL FFF     +++AASEFQTLTLR LP PSPLP     F   ++L+S+  A LT++L
Sbjct: 8   KYLLLFFF-----ISTAASEFQTLTLRSLPTPSPLPL----FPDSQSLQSSPDAPLTLDL 67

Query: 66  HHLDSLSTNKTPSDLFNLRLHRDALRVDSLTSLTAGRSRTPLRRAGFSSSVISGLAQGSG 125
           HHLDSLS NKTP+DLFNLRLHRD LRV +L S          R AGFSSSV+SGL+QGSG
Sbjct: 68  HHLDSLSLNKTPTDLFNLRLHRDTLRVHALNS----------RAAGFSSSVVSGLSQGSG 127

Query: 126 EYFTRLGVGTPPRYIFMVLDTGSDVVWLQCSPCRKCYSQSDPIFNPFKSKSFAGIPCSSP 185
           EYFTRLGVGTPPRY++MVLDTGSDVVWLQCSPCRKCYSQSDPIFNP+KSKSFAGIPCSSP
Sbjct: 128 EYFTRLGVGTPPRYLYMVLDTGSDVVWLQCSPCRKCYSQSDPIFNPYKSKSFAGIPCSSP 187

Query: 186 LCRRLDSSGCNTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDNEGLF 245
           LCRRLDSSGC+TRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGH NEGLF
Sbjct: 188 LCRRLDSSGCSTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHHNEGLF 247

Query: 246 VGAAGLLGLGRGRFSFPSQTGLRFNHKFSYCLVDRSASSKPSSMVFGDAAISRLARFTPL 305
           VGAAGLLGLGRGR SFPSQTG+RFNHKFSYCLVDRSASSKPSSMVFGDAAISRLARFTPL
Sbjct: 248 VGAAGLLGLGRGRLSFPSQTGIRFNHKFSYCLVDRSASSKPSSMVFGDAAISRLARFTPL 307

Query: 306 ILNPKLETFYYVELIGISVGGVRVRGISASLFKLDSAGNGGVIIDSGTSVTRLTRPAYTA 365
           I NPKL+TFYYV LIGISVGGVRVRG+S SLFKLDSAGNGGVIIDSGTSVTRLTRPAYTA
Sbjct: 308 IRNPKLDTFYYVGLIGISVGGVRVRGVSPSLFKLDSAGNGGVIIDSGTSVTRLTRPAYTA 367

Query: 366 LRDAFRAGASHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMALPATNYLIPVDD 425
           LRDAFR GA HLKRGPEFSLFDTCYDLSGQS+VKVPTVVLHFRGADMALPATNYLIPVD+
Sbjct: 368 LRDAFRVGARHLKRGPEFSLFDTCYDLSGQSSVKVPTVVLHFRGADMALPATNYLIPVDE 427

Query: 426 SGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT 469
           +GSFCFAFAGT+SGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT
Sbjct: 428 NGSFCFAFAGTISGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT 453

BLAST of Carg04941 vs. ExPASy TrEMBL
Match: A0A1S3CHC4 (aspartyl protease family protein 2 OS=Cucumis melo OX=3656 GN=LOC103500464 PE=3 SV=1)

HSP 1 Score: 793.9 bits (2049), Expect = 3.7e-226
Identity = 402/465 (86.45%), Postives = 424/465 (91.18%), Query Frame = 0

Query: 6   RYLLFFFFFFFAAVASAASEFQTLTLRRLPIPSPLPFPQPQFDTQETLEST--AALTVEL 65
           +YLL F+F     ++SAASEFQTLTLR LP PSPL      F   E+L+S+  AALT++L
Sbjct: 8   KYLLLFYF-----ISSAASEFQTLTLRSLPTPSPLSL----FPDSESLQSSPAAALTLDL 67

Query: 66  HHLDSLSTNKTPSDLFNLRLHRDALRVDSLTSLTAGRSRTPLRRAGFSSSVISGLAQGSG 125
           HHLDSLS NKTP+DLFNLRLHRDALRV +LTS  A          GFSSSVISGLAQGSG
Sbjct: 68  HHLDSLSLNKTPTDLFNLRLHRDALRVHALTSRAA---------PGFSSSVISGLAQGSG 127

Query: 126 EYFTRLGVGTPPRYIFMVLDTGSDVVWLQCSPCRKCYSQSDPIFNPFKSKSFAGIPCSSP 185
           EYFTRLGVGTPPRY++MVLDTGSDVVWLQCSPCRKCYSQSDPIFNP+KSKSFAGIPCSSP
Sbjct: 128 EYFTRLGVGTPPRYLYMVLDTGSDVVWLQCSPCRKCYSQSDPIFNPYKSKSFAGIPCSSP 187

Query: 186 LCRRLDSSGCNTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHDNEGLF 245
           LCRRLDSSGC+TRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGH NEGLF
Sbjct: 188 LCRRLDSSGCSTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGHHNEGLF 247

Query: 246 VGAAGLLGLGRGRFSFPSQTGLRFNHKFSYCLVDRSASSKPSSMVFGDAAISRLARFTPL 305
           VGAAGLLGLGRGR SFPSQTG+RFN KFSYCLVDRSASSKPSSMVFGDAAISRLARFTPL
Sbjct: 248 VGAAGLLGLGRGRLSFPSQTGIRFNRKFSYCLVDRSASSKPSSMVFGDAAISRLARFTPL 307

Query: 306 ILNPKLETFYYVELIGISVGGVRVRGISASLFKLDSAGNGGVIIDSGTSVTRLTRPAYTA 365
           I NPKL+TFYYVELIGISVGGVRVRG+  SLFKLDSAGNGGVIIDSGTSVTRLTRPAYTA
Sbjct: 308 IRNPKLDTFYYVELIGISVGGVRVRGVYPSLFKLDSAGNGGVIIDSGTSVTRLTRPAYTA 367

Query: 366 LRDAFRAGASHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMALPATNYLIPVDD 425
           LRDAFRAGA HLKRGPEFSLFDTCYDLSGQS+VKVPTVVLHFRGADM LPATNYLIPVD+
Sbjct: 368 LRDAFRAGARHLKRGPEFSLFDTCYDLSGQSSVKVPTVVLHFRGADMPLPATNYLIPVDE 427

Query: 426 SGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT 469
           +GSFCFAFAGT+SGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT
Sbjct: 428 NGSFCFAFAGTISGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGCT 454

BLAST of Carg04941 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 671.8 bits (1732), Expect = 4.0e-193
Identity = 341/478 (71.34%), Postives = 394/478 (82.43%), Query Frame = 0

Query: 8   LLFFFFFFFAAVASAAS--EFQTL--TLRRLPIPSPLPFPQPQFDTQETL---------- 67
           LLF   FFF ++ S +S   FQTL      LP  SP+ F QP  D++  L          
Sbjct: 8   LLFSLCFFFLSLPSFSSLPSFQTLFPNSHSLPCASPVSF-QPDSDSESLLESEFESGSDS 67

Query: 68  ESTAALTVELHHLDSLSTNKTPSDLFNLRLHRDALRVDSLTSLTA---GRSRTPL-RRAG 127
           ES++++T+ L H+D+LS+NKTP +LF+ RL RD+ RV S+ +L A   GR+ T   R  G
Sbjct: 68  ESSSSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAPRPGG 127

Query: 128 FSSSVISGLAQGSGEYFTRLGVGTPPRYIFMVLDTGSDVVWLQCSPCRKCYSQSDPIFNP 187
           FSSSV+SGL+QGSGEYFTRLGVGTP RY++MVLDTGSD+VWLQC+PCR+CYSQSDPIF+P
Sbjct: 128 FSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFDP 187

Query: 188 FKSKSFAGIPCSSPLCRRLDSSGCNTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIA 247
            KSK++A IPCSSP CRRLDS+GCNTRR TCLYQVSYGDGSFT GDF+TETLTFR N++ 
Sbjct: 188 RKSKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRRNRVK 247

Query: 248 KVALGCGHDNEGLFVGAAGLLGLGRGRFSFPSQTGLRFNHKFSYCLVDRSASSKPSSMVF 307
            VALGCGHDNEGLFVGAAGLLGLG+G+ SFP QTG RFN KFSYCLVDRSASSKPSS+VF
Sbjct: 248 GVALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVF 307

Query: 308 GDAAISRLARFTPLILNPKLETFYYVELIGISVGGVRVRGISASLFKLDSAGNGGVIIDS 367
           G+AA+SR+ARFTPL+ NPKL+TFYYV L+GISVGG RV G++ASLFKLD  GNGGVIIDS
Sbjct: 308 GNAAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDS 367

Query: 368 GTSVTRLTRPAYTALRDAFRAGASHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGAD 427
           GTSVTRL RPAY A+RDAFR GA  LKR P+FSLFDTC+DLS  + VKVPTVVLHFRGAD
Sbjct: 368 GTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHFRGAD 427

Query: 428 MALPATNYLIPVDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGC 468
           ++LPATNYLIPVD +G FCFAFAGTM GLSIIGNIQQQGFRVVYDLA SR+GFAP GC
Sbjct: 428 VSLPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGC 484

BLAST of Carg04941 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 603.6 bits (1555), Expect = 1.4e-172
Identity = 313/475 (65.89%), Postives = 373/475 (78.53%), Query Frame = 0

Query: 8   LLFFFFFFFAAVASAASEFQTLTLRRLPIPSPLPFPQPQFDTQETL-ESTAALTVELHHL 67
           L F  F      +SA+S++QTL +  LP  + L +P+ +  T E+L EST +L+V L H+
Sbjct: 9   LAFSVFAVLFFTSSASSQYQTLVVNTLPSSATLSWPESESLTDESLSESTTSLSVHLSHV 68

Query: 68  DSLS--TNKTPSDLFNLRLHRDALRVDSLTSLTA---GRS---RTPLRRAGFSSSVISGL 127
           D+LS  ++ +P+DLFNLRL RD+LRV S+TSL A   GR+   RTP    GFS +VISGL
Sbjct: 69  DALSSFSDASPADLFNLRLQRDSLRVKSITSLAAVSTGRNATKRTPRTAGGFSGAVISGL 128

Query: 128 AQGSGEYFTRLGVGTPPRYIFMVLDTGSDVVWLQCSPCRKCYSQSDPIFNPFKSKSFAGI 187
           +QGSGEYF RLGVGTP   ++MVLDTGSDVVWLQCSPC+ CY+Q+D IF+P KSK+FA +
Sbjct: 129 SQGSGEYFMRLGVGTPATNVYMVLDTGSDVVWLQCSPCKACYNQTDAIFDPKKSKTFATV 188

Query: 188 PCSSPLCRRL-DSSGCNTRR-HTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCG 247
           PC S LCRRL DSS C TRR  TCLYQVSYGDGSFT GDF+TETLTF G ++  V LGCG
Sbjct: 189 PCGSRLCRRLDDSSECVTRRSKTCLYQVSYGDGSFTEGDFSTETLTFHGARVDHVPLGCG 248

Query: 248 HDNEGLFVGAAGLLGLGRGRFSFPSQTGLRFNHKFSYCLVDR----SASSKPSSMVFGDA 307
           HDNEGLFVGAAGLLGLGRG  SFPSQT  R+N KFSYCLVDR    S+S  PS++VFG+A
Sbjct: 249 HDNEGLFVGAAGLLGLGRGGLSFPSQTKNRYNGKFSYCLVDRTSSGSSSKPPSTIVFGNA 308

Query: 308 AISRLARFTPLILNPKLETFYYVELIGISVGGVRVRGISASLFKLDSAGNGGVIIDSGTS 367
           A+ + + FTPL+ NPKL+TFYY++L+GISVGG RV G+S S FKLD+ GNGGVIIDSGTS
Sbjct: 309 AVPKTSVFTPLLTNPKLDTFYYLQLLGISVGGSRVPGVSESQFKLDATGNGGVIIDSGTS 368

Query: 368 VTRLTRPAYTALRDAFRAGASHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHFRGADMAL 427
           VTRLT+PAY ALRDAFR GA+ LKR P +SLFDTC+DLSG + VKVPTVV HF G +++L
Sbjct: 369 VTRLTQPAYVALRDAFRLGATKLKRAPSYSLFDTCFDLSGMTTVKVPTVVFHFGGGEVSL 428

Query: 428 PATNYLIPVDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGC 468
           PA+NYLIPV+  G FCFAFAGTM  LSIIGNIQQQGFRV YDL GSR+GF  R C
Sbjct: 429 PASNYLIPVNTEGRFCFAFAGTMGSLSIIGNIQQQGFRVAYDLVGSRVGFLSRAC 483

BLAST of Carg04941 vs. TAIR 10
Match: AT3G18490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 400.2 bits (1027), Expect = 2.3e-111
Identity = 230/469 (49.04%), Postives = 294/469 (62.69%), Query Frame = 0

Query: 19  VASAASEFQTLTLRRLPIPSPLPFPQPQ-FDTQETLESTAALTVELHHLDSL--STNKTP 78
           V S+  + QT+ L   P  S L   +P+         S++ L++ELH  D+   S +K  
Sbjct: 40  VVSSLQQTQTI-LSLDPTRSSLTTTKPESLSDPVFFNSSSPLSLELHSRDTFVASQHKDY 99

Query: 79  SDLFNLRLHRDALRVDSLTS----LTAGRSRTPLR----------RAGFSSSVISGLAQG 138
             L   RL RD+ RV  + +       G  R+ L+              ++ V+SG +QG
Sbjct: 100 KSLTLSRLERDSSRVAGIVAKIRFAVEGVDRSDLKPVYNEDTRYQTEDLTTPVVSGASQG 159

Query: 139 SGEYFTRLGVGTPPRYIFMVLDTGSDVVWLQCSPCRKCYSQSDPIFNPFKSKSFAGIPCS 198
           SGEYF+R+GVGTP + +++VLDTGSDV W+QC PC  CY QSDP+FNP  S ++  + CS
Sbjct: 160 SGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVFNPTSSSTYKSLTCS 219

Query: 199 SPLCRRLDSSGCNTRRHTCLYQVSYGDGSFTTGDFATETLTF-RGNKIAKVALGCGHDNE 258
           +P C  L++S C  R + CLYQVSYGDGSFT G+ AT+T+TF    KI  VALGCGHDNE
Sbjct: 220 APQCSLLETSAC--RSNKCLYQVSYGDGSFTVGELATDTVTFGNSGKINNVALGCGHDNE 279

Query: 259 GLFVGAAGLLGLGRGRFSFPSQTGLRFNHKFSYCLVDRSASSKPSSMVFGDAAISRLARF 318
           GLF GAAGLLGLG G  S  +Q        FSYCLVDR  S K SS+ F    +      
Sbjct: 280 GLFTGAAGLLGLGGGVLSITNQMKAT---SFSYCLVDRD-SGKSSSLDFNSVQLGGGDAT 339

Query: 319 TPLILNPKLETFYYVELIGISVGGVRVRGISASLFKLDSAGNGGVIIDSGTSVTRLTRPA 378
            PL+ N K++TFYYV L G SVGG +V  +  ++F +D++G+GGVI+D GT+VTRL   A
Sbjct: 340 APLLRNKKIDTFYYVGLSGFSVGGEKV-VLPDAIFDVDASGSGGVILDCGTAVTRLQTQA 399

Query: 379 YTALRDAFRAGASHLKRG-PEFSLFDTCYDLSGQSAVKVPTVVLHFRGA-DMALPATNYL 438
           Y +LRDAF     +LK+G    SLFDTCYD S  S VKVPTV  HF G   + LPA NYL
Sbjct: 400 YNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLSTVKVPTVAFHFTGGKSLDLPAKNYL 459

Query: 439 IPVDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGC 468
           IPVDDSG+FCFAFA T S LSIIGN+QQQG R+ YDL+ + IG +   C
Sbjct: 460 IPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

BLAST of Carg04941 vs. TAIR 10
Match: AT3G20015.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 392.9 bits (1008), Expect = 3.6e-109
Identity = 211/471 (44.80%), Postives = 296/471 (62.85%), Query Frame = 0

Query: 8   LLFFFFFFFAAVASAAS----EFQTLTLRRLPIPSPLPFPQPQFDTQETLESTAALTVEL 67
           L FFF      ++S++S    +FQ + + + P+      P    +T  + ES++  T+ L
Sbjct: 5   LFFFFLHLHLHLSSSSSISFPDFQIIDVLQPPLTVTATLPDFN-NTHFSDESSSKYTLRL 64

Query: 68  HHLDSLS--TNKTPSDLFNLRLHRDALRVDSLTSLTAGR----SRTPLRRAGFSSSVISG 127
            H D     T +      + R+ RD  RV ++    +G+    S +      F S ++SG
Sbjct: 65  LHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKVIPSSDSRYEVNDFGSDIVSG 124

Query: 128 LAQGSGEYFTRLGVGTPPRYIFMVLDTGSDVVWLQCSPCRKCYSQSDPIFNPFKSKSFAG 187
           + QGSGEYF R+GVG+PPR  +MV+D+GSD+VW+QC PC+ CY QSDP+F+P KS S+ G
Sbjct: 125 MDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSDPVFDPAKSGSYTG 184

Query: 188 IPCSSPLCRRLDSSGCNTRRHTCLYQVSYGDGSFTTGDFATETLTFRGNKIAKVALGCGH 247
           + C S +C R+++SGC++    C Y+V YGDGS+T G  A ETLTF    +  VA+GCGH
Sbjct: 185 VSCGSSVCDRIENSGCHS--GGCRYEVMYGDGSYTKGTLALETLTFAKTVVRNVAMGCGH 244

Query: 248 DNEGLFVGAAGLLGLGRGRFSFPSQTGLRFNHKFSYCLVDRSASSKPSSMVFGDAAISRL 307
            N G+F+GAAGLLG+G G  SF  Q   +    F YCLV R   S   S+VFG  A+   
Sbjct: 245 RNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDS-TGSLVFGREALPVG 304

Query: 308 ARFTPLILNPKLETFYYVELIGISVGGVRVRGISASLFKLDSAGNGGVIIDSGTSVTRLT 367
           A + PL+ NP+  +FYYV L G+ VGGVR+  +   +F L   G+GGV++D+GT+VTRL 
Sbjct: 305 ASWVPLVRNPRAPSFYYVGLKGLGVGGVRI-PLPDGVFDLTETGDGGVVMDTGTAVTRLP 364

Query: 368 RPAYTALRDAFRAGASHLKRGPEFSLFDTCYDLSGQSAVKVPTVVLHF-RGADMALPATN 427
             AY A RD F++  ++L R    S+FDTCYDLSG  +V+VPTV  +F  G  + LPA N
Sbjct: 365 TAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPVLTLPARN 424

Query: 428 YLIPVDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDLAGSRIGFAPRGC 468
           +L+PVDDSG++CFAFA + +GLSIIGNIQQ+G +V +D A   +GF P  C
Sbjct: 425 FLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

BLAST of Carg04941 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 392.5 bits (1007), Expect = 4.7e-109
Identity = 228/492 (46.34%), Postives = 297/492 (60.37%), Query Frame = 0

Query: 5   PRYLLFFFFFFFAAVASAAS----EFQTLT---------LRRLPIPSPLPFPQPQFDTQE 64
           P Y  FFF FF  + +S  S    E  T T         + R    S     Q +   ++
Sbjct: 3   PNYSFFFFIFFLTSHSSVFSRILPETSTTTTSILNVADSIHRTKYTSSFRLNQQE---EQ 62

Query: 65  TLESTAALTVELHHLDSL--STNKTPSDLFNLRLHRDALRVDSL-------------TSL 124
           T  ++++ +++LH   S+  + +     L   RL+RD  RV SL               L
Sbjct: 63  THSASSSFSLQLHSRVSVRGTEHSDYKSLTLARLNRDTARVKSLITRLDLAINNISKADL 122

Query: 125 TAGRSRTPLRRAGFSSSVISGLAQGSGEYFTRLGVGTPPRYIFMVLDTGSDVVWLQCSPC 184
               +          + +ISG  QGSGEYFTR+G+G P R ++MVLDTGSDV WLQC+PC
Sbjct: 123 KPISTMYTTEEQDIEAPLISGTTQGSGEYFTRVGIGKPAREVYMVLDTGSDVNWLQCTPC 182

Query: 185 RKCYSQSDPIFNPFKSKSFAGIPCSSPLCRRLDSSGCNTRRHTCLYQVSYGDGSFTTGDF 244
             CY Q++PIF P  S S+  + C +P C  L+ S C  R  TCLY+VSYGDGS+T GDF
Sbjct: 183 ADCYHQTEPIFEPSSSSSYEPLSCDTPQCNALEVSEC--RNATCLYEVSYGDGSYTVGDF 242

Query: 245 ATETLTFRGNKIAKVALGCGHDNEGLFVGAAGLLGLGRGRFSFPSQTGLRFNHKFSYCLV 304
           ATETLT     +  VA+GCGH NEGLFVGAAGLLGLG G  + PSQ        FSYCLV
Sbjct: 243 ATETLTIGSTLVQNVAVGCGHSNEGLFVGAAGLLGLGGGLLALPSQLN---TTSFSYCLV 302

Query: 305 DRSASSKPSSMVFGDAAISRLARFTPLILNPKLETFYYVELIGISVGGVRVRGISASLFK 364
           DR + S  S++ FG  ++S  A   PL+ N +L+TFYY+ L GISVGG  ++ I  S F+
Sbjct: 303 DRDSDS-ASTVDFG-TSLSPDAVVAPLLRNHQLDTFYYLGLTGISVGGELLQ-IPQSSFE 362

Query: 365 LDSAGNGGVIIDSGTSVTRLTRPAYTALRDAFRAGASHLKRGPEFSLFDTCYDLSGQSAV 424
           +D +G+GG+IIDSGT+VTRL    Y +LRD+F  G   L++    ++FDTCY+LS ++ V
Sbjct: 363 MDESGSGGIIIDSGTAVTRLQTEIYNSLRDSFVKGTLDLEKAAGVAMFDTCYNLSAKTTV 422

Query: 425 KVPTVVLHFRGADM-ALPATNYLIPVDDSGSFCFAFAGTMSGLSIIGNIQQQGFRVVYDL 468
           +VPTV  HF G  M ALPA NY+IPVD  G+FC AFA T S L+IIGN+QQQG RV +DL
Sbjct: 423 EVPTVAFHFPGGKMLALPAKNYMIPVDSVGTFCLAFAPTASSLAIIGNVQQQGTRVTFDL 482

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7025313.11.9e-269100.00Aspartyl protease family protein 2, partial [Cucurbita argyrosperma subsp. argyr... [more]
XP_022959948.18.8e-26799.15aspartyl protease family protein 2 [Cucurbita moschata][more]
XP_023514169.12.8e-26598.93aspartyl protease family protein 2 [Cucurbita pepo subsp. pepo][more]
XP_023005015.15.0e-26298.08aspartyl protease family protein 2 [Cucurbita maxima][more]
KAG6592908.11.9e-24595.98Aspartyl protease family protein 2, partial [Cucurbita argyrosperma subsp. soror... [more]
Match NameE-valueIdentityDescription
Q9LNJ35.7e-19271.34Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ... [more]
Q9LS403.2e-11049.04Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q9LHE35.1e-10844.80Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q766C31.4e-7341.02Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S... [more]
Q8S9J61.8e-7340.76Aspartyl protease family protein At5g10770 OS=Arabidopsis thaliana OX=3702 GN=At... [more]
Match NameE-valueIdentityDescription
A0A6J1H7E04.3e-26799.15aspartyl protease family protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111460848... [more]
A0A6J1KW812.4e-26298.08aspartyl protease family protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111498134 P... [more]
A0A5A7U8Z24.3e-22786.67Aspartyl protease family protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A0A0K4G22.8e-22685.81Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G32935... [more]
A0A1S3CHC43.7e-22686.45aspartyl protease family protein 2 OS=Cucumis melo OX=3656 GN=LOC103500464 PE=3 ... [more]
Match NameE-valueIdentityDescription
AT1G01300.14.0e-19371.34Eukaryotic aspartyl protease family protein [more]
AT3G61820.11.4e-17265.89Eukaryotic aspartyl protease family protein [more]
AT3G18490.12.3e-11149.04Eukaryotic aspartyl protease family protein [more]
AT3G20015.13.6e-10944.80Eukaryotic aspartyl protease family protein [more]
AT1G25510.14.7e-10946.34Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001461Aspartic peptidase A1 familyPRINTSPR00792PEPSINcoord: 439..454
score: 26.83
coord: 345..356
score: 41.93
coord: 131..151
score: 40.67
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 30..467
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 312..463
e-value: 2.1E-36
score: 125.2
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 108..288
e-value: 5.3E-53
score: 181.9
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 289..468
e-value: 4.7E-54
score: 184.9
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 118..467
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 125..291
e-value: 5.8E-56
score: 189.5
NoneNo IPR availablePANTHERPTHR13683:SF761ASPARTYL PROTEASE FAMILY PROTEIN 2-LIKEcoord: 30..467
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 345..356
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 140..151
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 125..463
score: 46.264454
IPR033873CND41-likeCDDcd05472cnd41_likecoord: 124..467
e-value: 1.76389E-149
score: 425.917

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg04941-RACarg04941-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004190 aspartic-type endopeptidase activity