Carg04882 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg04882
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptioncoiled-coil domain-containing protein 12-like
LocationCarg_Chr13: 8772946 .. 8774246 (+)
RNA-Seq ExpressionCarg04882
SyntenyCarg04882
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGTAATCTTAGTATGAAATTTCGGAACTATGTTCCTCATGATAAGGAGCTTCAGGAGGGAAAGCTTGTTCCACCTGTATTGCCGAAGTTTGAGGACCCTGTTGCTACTGAACCTCCACAATTAAAAGAGGTAGTTTTTTCAGTTCTGCTTTTATATGTTCTTAAATTAACCATTGCTTGTACTTGTCGGATCTTTTGTATTAATTACATTCCACTGGAAAATTTGTAGGATCCATTTGTAAATATTGCTCCAAAGAAACCAAACTGGGATCTTAGAAGGGATGTGCAGAAGAAGCTTGATAAGCTAGAAAGACGTACTCAGAAAGCATTATATAAGCTTATGGGTGAGTAGGAATTTTGAATTAAAGCTCTGCCAACTTCATAAGTCTCTGTTTTATGTTCATTCTTTTACTCGCTTCATTTGGTATAGTAATTGGACATGGGAAATTTAATTTATTCGCTTTGTTTGTCTGGGAAGATATCTATCTCTAGTCAATTAGCATATATATAATATTGTTTTTATCTCCTAGTTCTATCTCGTGGTTCCATAAATATATATTTCATGTATTTTACAATACTCCACAAGTTTCTTATAAAAAAAATTTGAAGTCTATCAGTTGTGGATCTTTTCATTTAACAGTCATCCCTGCTGGGTAGTTAAAGTTGGGGGGATTTCTGGGTATCGAGGGAAGTTGTGGAGATTGGAGTAGAATAGGGCTAGTTTAAACTATAACCTTCCAGTCTTTAAAGGGAATTATTGAACCTAAGAAACTACAATCTTTAGGAGATGTAGGTTACTCTCATTACCGTATGCATTGGAAGTTACAAGAGTGATTATTGTGTGTATCATAAGATGGTAGAAAATGATATGAAGCTCCTAAAATTTAATAGTGGTTACAACTTAGAGCACCCCTTCTTACCTTTATGAACTTTATTTTCGTGCAATCTTTTGTAGAGGAACAGGAAAAACAAAAGCAGTCAGCTGAAGGAGACGATGACCAAAATATTACAGAATGATAGTATGCCGCCCAAGTTATGAGTTCTCCAGTATTACAGTCGCAATATTCACTACTATTCCGACGGCTTTATGTCTCATATGGTCAGATTTTCTGTAACCTTTCTGATCATTCTTTGCCGCAAATCTTTTGCTTTTAATTTATCTATTGTGGCTCTCATGGTGTTCCTTGACTAACTATGCCCCATAGCATATCTCACACTGGATAGTGATTTGTGATTTGTACCTATGCTAAAAATTACTTTGTACTTCATCTTATCTTCATTTTGAACCGTTTTCTTATTTTT

mRNA sequence

AGTAATCTTAGTATGAAATTTCGGAACTATGTTCCTCATGATAAGGAGCTTCAGGAGGGAAAGCTTGTTCCACCTGTATTGCCGAAGTTTGAGGACCCTGTTGCTACTGAACCTCCACAATTAAAAGAGGATCCATTTGTAAATATTGCTCCAAAGAAACCAAACTGGGATCTTAGAAGGGATGTGCAGAAGAAGCTTGATAAGCTAGAAAGACGTACTCAGAAAGCATTATATAAGCTTATGGAGGAACAGGAAAAACAAAAGCAGTCAGCTGAAGGAGACGATGACCAAAATATTACAGAATGATAGTATGCCGCCCAAGTTATGAGTTCTCCAGTATTACAGTCGCAATATTCACTACTATTCCGACGGCTTTATGTCTCATATGGTCAGATTTTCTGTAACCTTTCTGATCATTCTTTGCCGCAAATCTTTTGCTTTTAATTTATCTATTGTGGCTCTCATGGTGTTCCTTGACTAACTATGCCCCATAGCATATCTCACACTGGATAGTGATTTGTGATTTGTACCTATGCTAAAAATTACTTTGTACTTCATCTTATCTTCATTTTGAACCGTTTTCTTATTTTT

Coding sequence (CDS)

ATGAAATTTCGGAACTATGTTCCTCATGATAAGGAGCTTCAGGAGGGAAAGCTTGTTCCACCTGTATTGCCGAAGTTTGAGGACCCTGTTGCTACTGAACCTCCACAATTAAAAGAGGATCCATTTGTAAATATTGCTCCAAAGAAACCAAACTGGGATCTTAGAAGGGATGTGCAGAAGAAGCTTGATAAGCTAGAAAGACGTACTCAGAAAGCATTATATAAGCTTATGGAGGAACAGGAAAAACAAAAGCAGTCAGCTGAAGGAGACGATGACCAAAATATTACAGAATGA

Protein sequence

MKFRNYVPHDKELQEGKLVPPVLPKFEDPVATEPPQLKEDPFVNIAPKKPNWDLRRDVQKKLDKLERRTQKALYKLMEEQEKQKQSAEGDDDQNITE
Homology
BLAST of Carg04882 vs. NCBI nr
Match: KAG7019878.1 (Coiled-coil domain-containing protein 12 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 191.4 bits (485), Expect = 3.6e-45
Identity = 97/97 (100.00%), Postives = 97/97 (100.00%), Query Frame = 0

Query: 1  MKFRNYVPHDKELQEGKLVPPVLPKFEDPVATEPPQLKEDPFVNIAPKKPNWDLRRDVQK 60
          MKFRNYVPHDKELQEGKLVPPVLPKFEDPVATEPPQLKEDPFVNIAPKKPNWDLRRDVQK
Sbjct: 1  MKFRNYVPHDKELQEGKLVPPVLPKFEDPVATEPPQLKEDPFVNIAPKKPNWDLRRDVQK 60

Query: 61 KLDKLERRTQKALYKLMEEQEKQKQSAEGDDDQNITE 98
          KLDKLERRTQKALYKLMEEQEKQKQSAEGDDDQNITE
Sbjct: 61 KLDKLERRTQKALYKLMEEQEKQKQSAEGDDDQNITE 97

BLAST of Carg04882 vs. NCBI nr
Match: XP_023520254.1 (coiled-coil domain-containing protein 12-like [Cucurbita pepo subsp. pepo] >XP_023520255.1 coiled-coil domain-containing protein 12-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 191.4 bits (485), Expect = 3.6e-45
Identity = 97/97 (100.00%), Postives = 97/97 (100.00%), Query Frame = 0

Query: 1   MKFRNYVPHDKELQEGKLVPPVLPKFEDPVATEPPQLKEDPFVNIAPKKPNWDLRRDVQK 60
           MKFRNYVPHDKELQEGKLVPPVLPKFEDPVATEPPQLKEDPFVNIAPKKPNWDLRRDVQK
Sbjct: 60  MKFRNYVPHDKELQEGKLVPPVLPKFEDPVATEPPQLKEDPFVNIAPKKPNWDLRRDVQK 119

Query: 61  KLDKLERRTQKALYKLMEEQEKQKQSAEGDDDQNITE 98
           KLDKLERRTQKALYKLMEEQEKQKQSAEGDDDQNITE
Sbjct: 120 KLDKLERRTQKALYKLMEEQEKQKQSAEGDDDQNITE 156

BLAST of Carg04882 vs. NCBI nr
Match: KAG6584286.1 (Coiled-coil domain-containing protein 12, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 191.4 bits (485), Expect = 3.6e-45
Identity = 97/97 (100.00%), Postives = 97/97 (100.00%), Query Frame = 0

Query: 1   MKFRNYVPHDKELQEGKLVPPVLPKFEDPVATEPPQLKEDPFVNIAPKKPNWDLRRDVQK 60
           MKFRNYVPHDKELQEGKLVPPVLPKFEDPVATEPPQLKEDPFVNIAPKKPNWDLRRDVQK
Sbjct: 57  MKFRNYVPHDKELQEGKLVPPVLPKFEDPVATEPPQLKEDPFVNIAPKKPNWDLRRDVQK 116

Query: 61  KLDKLERRTQKALYKLMEEQEKQKQSAEGDDDQNITE 98
           KLDKLERRTQKALYKLMEEQEKQKQSAEGDDDQNITE
Sbjct: 117 KLDKLERRTQKALYKLMEEQEKQKQSAEGDDDQNITE 153

BLAST of Carg04882 vs. NCBI nr
Match: XP_022923663.1 (coiled-coil domain-containing protein 12-like [Cucurbita moschata] >XP_022923664.1 coiled-coil domain-containing protein 12-like [Cucurbita moschata])

HSP 1 Score: 189.1 bits (479), Expect = 1.8e-44
Identity = 96/97 (98.97%), Postives = 96/97 (98.97%), Query Frame = 0

Query: 1   MKFRNYVPHDKELQEGKLVPPVLPKFEDPVATEPPQLKEDPFVNIAPKKPNWDLRRDVQK 60
           MKFRNYVPHDKELQEGKLVPP LPKFEDPVATEPPQLKEDPFVNIAPKKPNWDLRRDVQK
Sbjct: 60  MKFRNYVPHDKELQEGKLVPPELPKFEDPVATEPPQLKEDPFVNIAPKKPNWDLRRDVQK 119

Query: 61  KLDKLERRTQKALYKLMEEQEKQKQSAEGDDDQNITE 98
           KLDKLERRTQKALYKLMEEQEKQKQSAEGDDDQNITE
Sbjct: 120 KLDKLERRTQKALYKLMEEQEKQKQSAEGDDDQNITE 156

BLAST of Carg04882 vs. NCBI nr
Match: XP_023542240.1 (coiled-coil domain-containing protein 12-like [Cucurbita pepo subsp. pepo] >XP_023542241.1 coiled-coil domain-containing protein 12-like [Cucurbita pepo subsp. pepo] >XP_023542242.1 coiled-coil domain-containing protein 12-like [Cucurbita pepo subsp. pepo] >XP_023542243.1 coiled-coil domain-containing protein 12-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 187.2 bits (474), Expect = 6.7e-44
Identity = 95/97 (97.94%), Postives = 95/97 (97.94%), Query Frame = 0

Query: 1   MKFRNYVPHDKELQEGKLVPPVLPKFEDPVATEPPQLKEDPFVNIAPKKPNWDLRRDVQK 60
           MKFRNYVPHDKELQEGKL PPVLPKFEDPVATEPPQLKEDPFVNIAPKKPNWDLRRDVQK
Sbjct: 57  MKFRNYVPHDKELQEGKLAPPVLPKFEDPVATEPPQLKEDPFVNIAPKKPNWDLRRDVQK 116

Query: 61  KLDKLERRTQKALYKLMEEQEKQKQSAEGDDDQNITE 98
           KLDKLERRTQKALYKLMEEQEKQKQSAEGDDDQN TE
Sbjct: 117 KLDKLERRTQKALYKLMEEQEKQKQSAEGDDDQNDTE 153

BLAST of Carg04882 vs. ExPASy Swiss-Prot
Match: Q8R344 (Coiled-coil domain-containing protein 12 OS=Mus musculus OX=10090 GN=Ccdc12 PE=1 SV=2)

HSP 1 Score: 65.5 bits (158), Expect = 3.9e-10
Identity = 38/94 (40.43%), Postives = 61/94 (64.89%), Query Frame = 0

Query: 1   MKFRNYVPHDKELQEGKLVPPVLP-------KFEDPVATEPPQLKEDPFVNIAPKKPNWD 60
           ++ RNYVP D++L+  + VP   P       K +   A   P ++E    N+AP+KP+WD
Sbjct: 57  LRLRNYVPEDEDLKR-RRVPQAKPVAVEEKVKEQLEAAKPEPVIEEVDLANLAPRKPDWD 116

Query: 61  LRRDVQKKLDKLERRTQKALYKLMEEQEKQKQSA 88
           L+RDV KKL+KLE+RTQ+A+ +L+ E+ K ++ +
Sbjct: 117 LKRDVAKKLEKLEKRTQRAIAELIRERLKGQEDS 149

BLAST of Carg04882 vs. ExPASy Swiss-Prot
Match: Q8WUD4 (Coiled-coil domain-containing protein 12 OS=Homo sapiens OX=9606 GN=CCDC12 PE=1 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 8.6e-10
Identity = 37/94 (39.36%), Postives = 62/94 (65.96%), Query Frame = 0

Query: 1   MKFRNYVPHDKELQEGKLVPPVLP-------KFEDPVATEPPQLKEDPFVNIAPKKPNWD 60
           ++ RNYVP D++L++ + VP   P       K +   A   P ++E    N+AP+KP+WD
Sbjct: 57  LRLRNYVPEDEDLKK-RRVPQAKPVAVEEKVKEQLEAAKPEPVIEEVDLANLAPRKPDWD 116

Query: 61  LRRDVQKKLDKLERRTQKALYKLMEEQEKQKQSA 88
           L+RDV KKL+KL++RTQ+A+ +L+ E+ K ++ +
Sbjct: 117 LKRDVAKKLEKLKKRTQRAIAELIRERLKGQEDS 149

BLAST of Carg04882 vs. ExPASy TrEMBL
Match: A0A6J1E719 (coiled-coil domain-containing protein 12-like OS=Cucurbita moschata OX=3662 GN=LOC111431306 PE=4 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 8.6e-45
Identity = 96/97 (98.97%), Postives = 96/97 (98.97%), Query Frame = 0

Query: 1   MKFRNYVPHDKELQEGKLVPPVLPKFEDPVATEPPQLKEDPFVNIAPKKPNWDLRRDVQK 60
           MKFRNYVPHDKELQEGKLVPP LPKFEDPVATEPPQLKEDPFVNIAPKKPNWDLRRDVQK
Sbjct: 60  MKFRNYVPHDKELQEGKLVPPELPKFEDPVATEPPQLKEDPFVNIAPKKPNWDLRRDVQK 119

Query: 61  KLDKLERRTQKALYKLMEEQEKQKQSAEGDDDQNITE 98
           KLDKLERRTQKALYKLMEEQEKQKQSAEGDDDQNITE
Sbjct: 120 KLDKLERRTQKALYKLMEEQEKQKQSAEGDDDQNITE 156

BLAST of Carg04882 vs. ExPASy TrEMBL
Match: A0A6J1GST8 (coiled-coil domain-containing protein 12-like OS=Cucurbita moschata OX=3662 GN=LOC111457177 PE=4 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 3.3e-44
Identity = 95/97 (97.94%), Postives = 95/97 (97.94%), Query Frame = 0

Query: 1   MKFRNYVPHDKELQEGKLVPPVLPKFEDPVATEPPQLKEDPFVNIAPKKPNWDLRRDVQK 60
           MKFRNYVPHDKELQEGKL PPVLPKFEDPVATEPPQLKEDPFVNIAPKKPNWDLRRDVQK
Sbjct: 57  MKFRNYVPHDKELQEGKLAPPVLPKFEDPVATEPPQLKEDPFVNIAPKKPNWDLRRDVQK 116

Query: 61  KLDKLERRTQKALYKLMEEQEKQKQSAEGDDDQNITE 98
           KLDKLERRTQKALYKLMEEQEKQKQSAEGDDDQN TE
Sbjct: 117 KLDKLERRTQKALYKLMEEQEKQKQSAEGDDDQNDTE 153

BLAST of Carg04882 vs. ExPASy TrEMBL
Match: A0A6J1JYP1 (coiled-coil domain-containing protein 12-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490058 PE=4 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 4.3e-44
Identity = 95/97 (97.94%), Postives = 95/97 (97.94%), Query Frame = 0

Query: 1   MKFRNYVPHDKELQEGKLVPPVLPKFEDPVATEPPQLKEDPFVNIAPKKPNWDLRRDVQK 60
           MKFRNYVPHDKELQEGKL PPVLPKFEDPVATEPPQLKEDPFVNIAPKKPNWDLRRDVQK
Sbjct: 57  MKFRNYVPHDKELQEGKLAPPVLPKFEDPVATEPPQLKEDPFVNIAPKKPNWDLRRDVQK 116

Query: 61  KLDKLERRTQKALYKLMEEQEKQKQSAEGDDDQNITE 98
           KLDKLERRTQKALYKLMEEQEKQKQSAEGDDDQN TE
Sbjct: 117 KLDKLERRTQKALYKLMEEQEKQKQSAEGDDDQNGTE 153

BLAST of Carg04882 vs. ExPASy TrEMBL
Match: A0A1S3ATB1 (coiled-coil domain-containing protein 12 OS=Cucumis melo OX=3656 GN=LOC103482658 PE=4 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 5.2e-42
Identity = 91/97 (93.81%), Postives = 92/97 (94.85%), Query Frame = 0

Query: 1   MKFRNYVPHDKELQEGKLVPPVLPKFEDPVATEPPQLKEDPFVNIAPKKPNWDLRRDVQK 60
           MKFRNYVPHDKELQEGKL PPVLPKFEDPV TEPPQ KEDPFVNIAPKKPNWDLRRDVQK
Sbjct: 57  MKFRNYVPHDKELQEGKLAPPVLPKFEDPVTTEPPQQKEDPFVNIAPKKPNWDLRRDVQK 116

Query: 61  KLDKLERRTQKALYKLMEEQEKQKQSAEGDDDQNITE 98
           KLDKLERRTQKALYKLMEEQEKQKQ+AEGDDDQN  E
Sbjct: 117 KLDKLERRTQKALYKLMEEQEKQKQAAEGDDDQNGAE 153

BLAST of Carg04882 vs. ExPASy TrEMBL
Match: A0A5D3BJ95 (Coiled-coil domain-containing protein 12 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002830 PE=4 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 5.2e-42
Identity = 91/97 (93.81%), Postives = 92/97 (94.85%), Query Frame = 0

Query: 1   MKFRNYVPHDKELQEGKLVPPVLPKFEDPVATEPPQLKEDPFVNIAPKKPNWDLRRDVQK 60
           MKFRNYVPHDKELQEGKL PPVLPKFEDPV TEPPQ KEDPFVNIAPKKPNWDLRRDVQK
Sbjct: 57  MKFRNYVPHDKELQEGKLAPPVLPKFEDPVTTEPPQQKEDPFVNIAPKKPNWDLRRDVQK 116

Query: 61  KLDKLERRTQKALYKLMEEQEKQKQSAEGDDDQNITE 98
           KLDKLERRTQKALYKLMEEQEKQKQ+AEGDDDQN  E
Sbjct: 117 KLDKLERRTQKALYKLMEEQEKQKQAAEGDDDQNGAE 153

BLAST of Carg04882 vs. TAIR 10
Match: AT3G05070.1 (CONTAINS InterPro DOMAIN/s: mRNA splicing factor, Cwf18 (InterPro:IPR013169); Has 292 Blast hits to 292 proteins in 153 species: Archae - 0; Bacteria - 0; Metazoa - 118; Fungi - 83; Plants - 38; Viruses - 11; Other Eukaryotes - 42 (source: NCBI BLink). )

HSP 1 Score: 142.1 bits (357), Expect = 2.3e-34
Identity = 72/92 (78.26%), Postives = 80/92 (86.96%), Query Frame = 0

Query: 1   MKFRNYVPHDKELQEGKLVPPVLPKFEDPVATEPP--QLKEDPFVNIAPKKPNWDLRRDV 60
           MKFRNYVP  KELQ+GKL PP LPKFEDP+   PP  + KEDPFVNIAPKKPNWDLRRDV
Sbjct: 47  MKFRNYVPQAKELQDGKLAPPELPKFEDPIVALPPAVEKKEDPFVNIAPKKPNWDLRRDV 106

Query: 61  QKKLDKLERRTQKALYKLMEEQEKQKQSAEGD 91
           QKKLDKLERRTQKA++KLMEEQEK+++ AE D
Sbjct: 107 QKKLDKLERRTQKAMHKLMEEQEKEREMAEAD 138

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7019878.13.6e-45100.00Coiled-coil domain-containing protein 12 [Cucurbita argyrosperma subsp. argyrosp... [more]
XP_023520254.13.6e-45100.00coiled-coil domain-containing protein 12-like [Cucurbita pepo subsp. pepo] >XP_0... [more]
KAG6584286.13.6e-45100.00Coiled-coil domain-containing protein 12, partial [Cucurbita argyrosperma subsp.... [more]
XP_022923663.11.8e-4498.97coiled-coil domain-containing protein 12-like [Cucurbita moschata] >XP_022923664... [more]
XP_023542240.16.7e-4497.94coiled-coil domain-containing protein 12-like [Cucurbita pepo subsp. pepo] >XP_0... [more]
Match NameE-valueIdentityDescription
Q8R3443.9e-1040.43Coiled-coil domain-containing protein 12 OS=Mus musculus OX=10090 GN=Ccdc12 PE=1... [more]
Q8WUD48.6e-1039.36Coiled-coil domain-containing protein 12 OS=Homo sapiens OX=9606 GN=CCDC12 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1E7198.6e-4598.97coiled-coil domain-containing protein 12-like OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1GST83.3e-4497.94coiled-coil domain-containing protein 12-like OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1JYP14.3e-4497.94coiled-coil domain-containing protein 12-like isoform X1 OS=Cucurbita maxima OX=... [more]
A0A1S3ATB15.2e-4293.81coiled-coil domain-containing protein 12 OS=Cucumis melo OX=3656 GN=LOC103482658... [more]
A0A5D3BJ955.2e-4293.81Coiled-coil domain-containing protein 12 OS=Cucumis melo var. makuwa OX=1194695 ... [more]
Match NameE-valueIdentityDescription
AT3G05070.12.3e-3478.26CONTAINS InterPro DOMAIN/s: mRNA splicing factor, Cwf18 (InterPro:IPR013169); Ha... [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 55..90
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 76..97
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 76..91
IPR013169mRNA splicing factor Cwf18-likePFAMPF08315cwf18coord: 1..79
e-value: 5.1E-26
score: 91.9
IPR013169mRNA splicing factor Cwf18-likePANTHERPTHR31551PRE-MRNA-SPLICING FACTOR CWF18coord: 1..88

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg04882-RACarg04882-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0071014 post-mRNA release spliceosomal complex
cellular_component GO:0005684 U2-type spliceosomal complex