Carg04878 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg04878
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	kunitz trypsin inhibitor 2
Location	Carg_Chr13: 8793957 .. 8794568 (+)
RNA-Seq Expression	Carg04878
Synteny	Carg04878

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGAATTTCGCGTTACTGTCTTTTCTTTTCATTCTCCTTGCCTCTGAGGTCCGCGTCTCCAGAGCCGACGCCTCGCCGGACGCCGTCCGCGACATCGACGGCAAGAAGCTCCGAGCCGGCGTCAACTACTACATCCTCCCTGTTTTCCGCGGAAGAGGCGGCGGCCTAGCCCTAGGCAACCTCCAATCGGACAAATGTCCACTCAACGTCGTTCAAGAACAGTTTGAATTAATGAACGGCCTTCCCGCGGCATTTTTGCCTGTAAACCCTAAAAAAGGCGTCGTTCGAGTTTCGACGGATTTAAACGTCCAATTCGAGGCCAGTACGATCTGCGCCACATCGACGGTATGGAAATTGGACAAATTCGACGAATCGACGAAACAGTGGTTCATCACGATCGGCGGAACGAGAGGAAATCCAGGCGTCAAGACGGTCGACAATTGGTTCAAAATTGAGAAACACGGTAACGATTACAAATTCAAATTCTGTCCGACTGTGTGCGATTTCTGTAAAGTTATGTGCAGAGATGTTGGAATCTTCTTCAAGAACGGAAAGAGGGCTCTGGCTTTGAGCGATACGCCATTCCCTGTCATGTTCAAGGAAGTTTAA

mRNA sequence

ATGAAGAATTTCGCGTTACTGTCTTTTCTTTTCATTCTCCTTGCCTCTGAGGTCCGCGTCTCCAGAGCCGACGCCTCGCCGGACGCCGTCCGCGACATCGACGGCAAGAAGCTCCGAGCCGGCGTCAACTACTACATCCTCCCTGTTTTCCGCGGAAGAGGCGGCGGCCTAGCCCTAGGCAACCTCCAATCGGACAAATGTCCACTCAACGTCGTTCAAGAACAGTTTGAATTAATGAACGGCCTTCCCGCGGCATTTTTGCCTGTAAACCCTAAAAAAGGCGTCGTTCGAGTTTCGACGGATTTAAACGTCCAATTCGAGGCCAGTACGATCTGCGCCACATCGACGGTATGGAAATTGGACAAATTCGACGAATCGACGAAACAGTGGTTCATCACGATCGGCGGAACGAGAGGAAATCCAGGCGTCAAGACGGTCGACAATTGGTTCAAAATTGAGAAACACGGTAACGATTACAAATTCAAATTCTGTCCGACTGTGTGCGATTTCTGTAAAGTTATGTGCAGAGATGTTGGAATCTTCTTCAAGAACGGAAAGAGGGCTCTGGCTTTGAGCGATACGCCATTCCCTGTCATGTTCAAGGAAGTTTAA

Coding sequence (CDS)

ATGAAGAATTTCGCGTTACTGTCTTTTCTTTTCATTCTCCTTGCCTCTGAGGTCCGCGTCTCCAGAGCCGACGCCTCGCCGGACGCCGTCCGCGACATCGACGGCAAGAAGCTCCGAGCCGGCGTCAACTACTACATCCTCCCTGTTTTCCGCGGAAGAGGCGGCGGCCTAGCCCTAGGCAACCTCCAATCGGACAAATGTCCACTCAACGTCGTTCAAGAACAGTTTGAATTAATGAACGGCCTTCCCGCGGCATTTTTGCCTGTAAACCCTAAAAAAGGCGTCGTTCGAGTTTCGACGGATTTAAACGTCCAATTCGAGGCCAGTACGATCTGCGCCACATCGACGGTATGGAAATTGGACAAATTCGACGAATCGACGAAACAGTGGTTCATCACGATCGGCGGAACGAGAGGAAATCCAGGCGTCAAGACGGTCGACAATTGGTTCAAAATTGAGAAACACGGTAACGATTACAAATTCAAATTCTGTCCGACTGTGTGCGATTTCTGTAAAGTTATGTGCAGAGATGTTGGAATCTTCTTCAAGAACGGAAAGAGGGCTCTGGCTTTGAGCGATACGCCATTCCCTGTCATGTTCAAGGAAGTTTAA

Protein sequence

MKNFALLSFLFILLASEVRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLALGNLQSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVWKLDKFDESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDVGIFFKNGKRALALSDTPFPVMFKEV

Homology

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BLAST of Carg04878 vs. NCBI nr
Match: XP_022923703.1 (kunitz trypsin inhibitor 2 [Cucurbita moschata] >XP_023001808.1 kunitz trypsin inhibitor 2 [Cucurbita maxima] >KAG6584289.1 Kunitz trypsin inhibitor 5, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019882.1 Kunitz trypsin inhibitor 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 422.5 bits (1085), Expect = 2.0e-114
Identity = 203/203 (100.00%), Postives = 203/203 (100.00%), Query Frame = 0


Query: 1   MKNFALLSFLFILLASEVRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLALG 60
           MKNFALLSFLFILLASEVRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLALG
Sbjct: 1   MKNFALLSFLFILLASEVRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLALG 60

Query: 61  NLQSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVWKL 120
           NLQSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVWKL
Sbjct: 61  NLQSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVWKL 120

Query: 121 DKFDESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDVGI 180
           DKFDESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDVGI
Sbjct: 121 DKFDESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDVGI 180

Query: 181 FFKNGKRALALSDTPFPVMFKEV 204
           FFKNGKRALALSDTPFPVMFKEV
Sbjct: 181 FFKNGKRALALSDTPFPVMFKEV 203

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BLAST of Carg04878 vs. NCBI nr
Match: XP_023520252.1 (kunitz trypsin inhibitor 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 417.9 bits (1073), Expect = 4.9e-113
Identity = 201/203 (99.01%), Postives = 201/203 (99.01%), Query Frame = 0


Query: 1   MKNFALLSFLFILLASEVRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLALG 60
           MKNFALLSFLFILLASEVRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLALG
Sbjct: 1   MKNFALLSFLFILLASEVRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLALG 60

Query: 61  NLQSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVWKL 120
           NLQSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVWKL
Sbjct: 61  NLQSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVWKL 120

Query: 121 DKFDESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDVGI 180
           DKFDESTKQWFITIGG RGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDVGI
Sbjct: 121 DKFDESTKQWFITIGGARGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDVGI 180

Query: 181 FFKNGKRALALSDTPFPVMFKEV 204
           FFKNGKRALALSDTPFPVMFK V
Sbjct: 181 FFKNGKRALALSDTPFPVMFKVV 203

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BLAST of Carg04878 vs. NCBI nr
Match: XP_038895203.1 (kunitz trypsin inhibitor 5-like [Benincasa hispida])

HSP 1 Score: 370.5 bits (950), Expect = 9.0e-99
Identity = 179/205 (87.32%), Postives = 188/205 (91.71%), Query Frame = 0


Query: 1   MKNFA-LLSFLFILLAS-EVRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLA 60
           MKNF  LLSFLFILLAS EVR SRADASP+AVRDIDGKKLRAGVNYYILPV RGRGGGL 
Sbjct: 1   MKNFGILLSFLFILLASTEVRFSRADASPEAVRDIDGKKLRAGVNYYILPVIRGRGGGLT 60

Query: 61  LGNLQSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVW 120
           L NLQS+ CP+NVVQEQFELMNG P  FLPVNPKKGVVRVSTDLNVQFEASTICATSTVW
Sbjct: 61  LSNLQSENCPVNVVQEQFELMNGFPTTFLPVNPKKGVVRVSTDLNVQFEASTICATSTVW 120

Query: 121 KLDKFDESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDV 180
           KLDK DEST QWF+TIGG+RGNPGV+TVDNWFKI KHG DYK  FCP+VCDFCKVMCRD+
Sbjct: 121 KLDKVDESTGQWFVTIGGSRGNPGVETVDNWFKIVKHGKDYKLVFCPSVCDFCKVMCRDI 180

Query: 181 GIFFKNGKRALALSDTPFPVMFKEV 204
           GIFFKNGKRALALSDTPFPVMFK+V
Sbjct: 181 GIFFKNGKRALALSDTPFPVMFKKV 205

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BLAST of Carg04878 vs. NCBI nr
Match: XP_008437058.1 (PREDICTED: miraculin-like [Cucumis melo] >TYJ99073.1 miraculin-like [Cucumis melo var. makuwa])

HSP 1 Score: 365.2 bits (936), Expect = 3.8e-97
Identity = 172/203 (84.73%), Postives = 185/203 (91.13%), Query Frame = 0


Query: 2   KNFALLSFLFILLAS-EVRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLALG 61
           KNF +  F+FILLAS E+R S ADASP+AV DIDGKKLRAGVNYYILPVFRGRGGGL LG
Sbjct: 3   KNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLG 62

Query: 62  NLQSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVWKL 121
           NLQS+ CP+NVVQEQFELMNG P  F PVNPKKGVVRVSTDLNVQF+ASTIC TSTVWKL
Sbjct: 63  NLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWKL 122

Query: 122 DKFDESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDVGI 181
           DKFDEST QWF+TIGG+RGNPGV+TVDNWFKIEKHG DYK  FCPTVC+FCKVMCRD+GI
Sbjct: 123 DKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGI 182

Query: 182 FFKNGKRALALSDTPFPVMFKEV 204
           FFKNGKRALALSDTPFPVMFK+V
Sbjct: 183 FFKNGKRALALSDTPFPVMFKKV 205

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BLAST of Carg04878 vs. NCBI nr
Match: XP_004137534.1 (kunitz trypsin inhibitor 5 [Cucumis sativus])

HSP 1 Score: 360.5 bits (924), Expect = 9.3e-96
Identity = 173/206 (83.98%), Postives = 185/206 (89.81%), Query Frame = 0


Query: 1   MKNFALL-SFLFILLASE--VRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGL 60
           MKNF +L  FLFILLAS   +R S ADASP+AV DIDGKKLRAGVNYYILPVFRGRGGGL
Sbjct: 1   MKNFGILFYFLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGL 60

Query: 61  ALGNLQSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTV 120
            LGNLQS+KCPLNVVQEQ E+MNG P  F PVNPKKGVVRVSTDLNVQFEASTIC TSTV
Sbjct: 61  TLGNLQSEKCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTV 120

Query: 121 WKLDKFDESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRD 180
           WKLDKFDEST QW +TIGG+RGNPGV+TVDNWFKIEKHG DYK  FCPTVC+FCKVMCRD
Sbjct: 121 WKLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRD 180

Query: 181 VGIFFKNGKRALALSDTPFPVMFKEV 204
           +GIFFKNG+RALALSDTPFPVMFK+V
Sbjct: 181 IGIFFKNGERALALSDTPFPVMFKKV 206

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BLAST of Carg04878 vs. ExPASy Swiss-Prot
Match: Q9LMU2 (Kunitz trypsin inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=KTI5 PE=2 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 8.5e-60
Identity = 108/197 (54.82%), Postives = 144/197 (73.10%), Query Frame = 0


Query: 7   LSFLFILLASEV--RVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLALGNLQS 66
           L ++F+LLA  +  R    +A+ + V+DI+GK L  GVNYYILPV RGRGGGL + NL++
Sbjct: 4   LLYIFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKT 63

Query: 67  DKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVWKLDKFD 126
           + CP +V+Q+QFE+  GLP  F P + K   + VSTD+N++F      + +++W+L  FD
Sbjct: 64  ETCPTSVIQDQFEVSQGLPVKFSPYD-KSRTIPVSTDVNIKF------SPTSIWELANFD 123

Query: 127 ESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDVGIFFKN 186
           E+TKQWFI+  G  GNPG KTVDNWFKI+K   DYK +FCPTVC+FCKV+CRDVG+F ++
Sbjct: 124 ETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFVQD 183

Query: 187 GKRALALSDTPFPVMFK 202
           GKR LALSD P  VMFK
Sbjct: 184 GKRRLALSDVPLKVMFK 193

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BLAST of Carg04878 vs. ExPASy Swiss-Prot
Match: P13087 (Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3)

HSP 1 Score: 205.3 bits (521), Expect = 6.5e-52
Identity = 108/216 (50.00%), Postives = 138/216 (63.89%), Query Frame = 0


Query: 1   MKNFALLSFLFILL------ASEVRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRG 60
           MK   +LS  F  +      A+   +S AD++P+ V DIDG+KLR G NYYI+PV R  G
Sbjct: 1   MKELTMLSLSFFFVSALLAAAANPLLSAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHG 60

Query: 61  GGLALGNLQSD---KCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTI 120
           GGL +     +    CP  VVQ + E+ +  P AF P NPK+ VVRVSTDLN+ F A   
Sbjct: 61  GGLTVSATTPNGTFVCPPRVVQTRKEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMP 120

Query: 121 C--ATSTVWKLDKFDESTKQWFITIGGTRGNPGVKTVDNWFKIEKH--GNDYKFKFCPTV 180
           C   +STVW+LDK+DEST Q+F+TIGG +GNPG +T+ +WFKIE+      YK  FCPTV
Sbjct: 121 CRWTSSTVWRLDKYDESTGQYFVTIGGVKGNPGPETISSWFKIEEFCGSGFYKLVFCPTV 180

Query: 181 CDFCKVMCRDVGIFF-KNGKRALALSDTPFPVMFKE 203
           C  CKV C DVGI+  + G+R LALSD PF   F +
Sbjct: 181 CGSCKVKCGDVGIYIDQKGRRRLALSDKPFAFEFNK 216

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BLAST of Carg04878 vs. ExPASy Swiss-Prot
Match: P32765 (21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 2.3e-41
Identity = 89/203 (43.84%), Postives = 121/203 (59.61%), Query Frame = 0


Query: 5   ALLSFLFILLASEVRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGR-GGGLALGNLQ 64
           A++  LF   +       A+A+   V D DG +L+ GV YY+L    G  GGGLALG   
Sbjct: 6   AVVLLLFAFTSKSYFFGVANAANSPVLDTDGDELQTGVQYYVLSSISGAGGGGLALGRAT 65

Query: 65  SDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFE--ASTICATSTVWKLD 124
              CP  VVQ + +L NG P  F   + K  VVRVSTD+N++F      +C+TSTVW+LD
Sbjct: 66  GQSCPEIVVQRRSDLDNGTPVIFSNADSKDDVVRVSTDVNIEFVPIRDRLCSTSTVWRLD 125

Query: 125 KFDESTKQWFITIGGTRGNPGVKTVDNWFKIEKHG-NDYKFKFCPTVCDFCKVMCRDVGI 184
            +D S  +W++T  G +G PG  T+ +WFKIEK G   YKF+FCP+VCD C  +C D+G 
Sbjct: 126 NYDNSAGKWWVTTDGVKGEPGPNTLCSWFKIEKAGVLGYKFRFCPSVCDSCTTLCSDIGR 185

Query: 185 FF-KNGKRALALSDTPFPVMFKE 203
               +G+  LALSD  +  MFK+
Sbjct: 186 HSDDDGQIRLALSDNEWAWMFKK 208

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BLAST of Carg04878 vs. ExPASy Swiss-Prot
Match: Q8RXD5 (Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 3.6e-34
Identity = 85/197 (43.15%), Postives = 109/197 (55.33%), Query Frame = 0


Query: 9   FLFILLASEVRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLALGNLQSDKCP 68
           F  +L  + V  S A     AV DIDG  +    +YY+LPV RGRGGGL L       CP
Sbjct: 12  FYLVLALTAVLASNAYG---AVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAGRGGQPCP 71

Query: 69  LNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFE-ASTICATSTVWKLDKFDEST 128
            ++VQE  E+  G+P  F     K   V  S +LN++ +  +TIC  ST W++ +FD   
Sbjct: 72  YDIVQESSEVDEGIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHER 131

Query: 129 KQWFITIGGTRGNPGVKTVDNWFKIEKHGND-YKFKFCPTVCDFCKVMCRDVGIFFKN-G 188
           KQ+F+  G      G  ++ ++FKIEK G D YKF FCP  CD     C DVGIF    G
Sbjct: 132 KQYFVVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCDSGNPKCSDVGIFIDELG 191

Query: 189 KRALALSDTPFPVMFKE 203
            R LALSD PF VMFK+
Sbjct: 192 VRRLALSDKPFLVMFKK 204

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BLAST of Carg04878 vs. ExPASy Swiss-Prot
Match: P80691 (Latex serine proteinase inhibitor OS=Carica papaya OX=3649 PE=1 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 7.5e-24
Identity = 63/162 (38.89%), Postives = 90/162 (55.56%), Query Frame = 0


Query: 26  SPDAVRDIDGKKLRAGVNYYILPVFRGRGGG--LALGNLQSDKCPLNVVQEQFELMNGLP 85
           +P  + DIDGK +  GV+Y+++    G GGG     G     KCPL+VVQ+ F+  NG P
Sbjct: 2   APKPIVDIDGKPVLYGVDYFVVSAIWGAGGGGLTVYGPGNKKKCPLSVVQDPFD--NGEP 61

Query: 86  AAFLPV-NPKKGVVRVSTDLNVQFEASTICATSTVWKLDKFDESTKQWFITIGGTRGNPG 145
             F  + N K  +V  S DLNV+F  +  C  +T WK+D+F      W +T+GG +G  G
Sbjct: 62  IIFSAIKNVKDNIVFESVDLNVKFNITINCNETTAWKVDRF-PGVIGWTVTLGGEKGYHG 121

Query: 146 VKTVDNWFKIEKHG--NDYKFKFCPTVCDFCKVMCRDVGIFF 183
            ++  + FKI+K G    YKF FCP+      + C +V IFF
Sbjct: 122 FESTHSMFKIKKAGLPFSYKFHFCPSYPRTRLIPCNNVDIFF 160

[Close]

BLAST of Carg04878 vs. ExPASy TrEMBL
Match: A0A6J1KRM6 (kunitz trypsin inhibitor 2 OS=Cucurbita maxima OX=3661 GN=LOC111495838 PE=3 SV=1)

HSP 1 Score: 422.5 bits (1085), Expect = 9.6e-115
Identity = 203/203 (100.00%), Postives = 203/203 (100.00%), Query Frame = 0


Query: 1   MKNFALLSFLFILLASEVRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLALG 60
           MKNFALLSFLFILLASEVRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLALG
Sbjct: 1   MKNFALLSFLFILLASEVRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLALG 60

Query: 61  NLQSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVWKL 120
           NLQSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVWKL
Sbjct: 61  NLQSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVWKL 120

Query: 121 DKFDESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDVGI 180
           DKFDESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDVGI
Sbjct: 121 DKFDESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDVGI 180

Query: 181 FFKNGKRALALSDTPFPVMFKEV 204
           FFKNGKRALALSDTPFPVMFKEV
Sbjct: 181 FFKNGKRALALSDTPFPVMFKEV 203

[Close]

BLAST of Carg04878 vs. ExPASy TrEMBL
Match: A0A6J1EAD6 (kunitz trypsin inhibitor 2 OS=Cucurbita moschata OX=3662 GN=LOC111431333 PE=3 SV=1)

HSP 1 Score: 422.5 bits (1085), Expect = 9.6e-115
Identity = 203/203 (100.00%), Postives = 203/203 (100.00%), Query Frame = 0


Query: 1   MKNFALLSFLFILLASEVRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLALG 60
           MKNFALLSFLFILLASEVRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLALG
Sbjct: 1   MKNFALLSFLFILLASEVRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLALG 60

Query: 61  NLQSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVWKL 120
           NLQSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVWKL
Sbjct: 61  NLQSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVWKL 120

Query: 121 DKFDESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDVGI 180
           DKFDESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDVGI
Sbjct: 121 DKFDESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDVGI 180

Query: 181 FFKNGKRALALSDTPFPVMFKEV 204
           FFKNGKRALALSDTPFPVMFKEV
Sbjct: 181 FFKNGKRALALSDTPFPVMFKEV 203

[Close]

BLAST of Carg04878 vs. ExPASy TrEMBL
Match: A0A5D3BIG0 (Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002770 PE=3 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 1.8e-97
Identity = 172/203 (84.73%), Postives = 185/203 (91.13%), Query Frame = 0


Query: 2   KNFALLSFLFILLAS-EVRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLALG 61
           KNF +  F+FILLAS E+R S ADASP+AV DIDGKKLRAGVNYYILPVFRGRGGGL LG
Sbjct: 3   KNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLG 62

Query: 62  NLQSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVWKL 121
           NLQS+ CP+NVVQEQFELMNG P  F PVNPKKGVVRVSTDLNVQF+ASTIC TSTVWKL
Sbjct: 63  NLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWKL 122

Query: 122 DKFDESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDVGI 181
           DKFDEST QWF+TIGG+RGNPGV+TVDNWFKIEKHG DYK  FCPTVC+FCKVMCRD+GI
Sbjct: 123 DKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGI 182

Query: 182 FFKNGKRALALSDTPFPVMFKEV 204
           FFKNGKRALALSDTPFPVMFK+V
Sbjct: 183 FFKNGKRALALSDTPFPVMFKKV 205

[Close]

BLAST of Carg04878 vs. ExPASy TrEMBL
Match: A0A1S3ASR3 (miraculin-like OS=Cucumis melo OX=3656 GN=LOC103482596 PE=3 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 1.8e-97
Identity = 172/203 (84.73%), Postives = 185/203 (91.13%), Query Frame = 0


Query: 2   KNFALLSFLFILLAS-EVRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLALG 61
           KNF +  F+FILLAS E+R S ADASP+AV DIDGKKLRAGVNYYILPVFRGRGGGL LG
Sbjct: 3   KNFGIFYFIFILLASTELRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGLTLG 62

Query: 62  NLQSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVWKL 121
           NLQS+ CP+NVVQEQFELMNG P  F PVNPKKGVVRVSTDLNVQF+ASTIC TSTVWKL
Sbjct: 63  NLQSEICPVNVVQEQFELMNGFPTTFHPVNPKKGVVRVSTDLNVQFDASTICVTSTVWKL 122

Query: 122 DKFDESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDVGI 181
           DKFDEST QWF+TIGG+RGNPGV+TVDNWFKIEKHG DYK  FCPTVC+FCKVMCRD+GI
Sbjct: 123 DKFDESTGQWFVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRDIGI 182

Query: 182 FFKNGKRALALSDTPFPVMFKEV 204
           FFKNGKRALALSDTPFPVMFK+V
Sbjct: 183 FFKNGKRALALSDTPFPVMFKKV 205

[Close]

BLAST of Carg04878 vs. ExPASy TrEMBL
Match: A0A0A0LTW2 (Tumor-related protein OS=Cucumis sativus OX=3659 GN=Csa_1G043200 PE=3 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 4.5e-96
Identity = 173/206 (83.98%), Postives = 185/206 (89.81%), Query Frame = 0


Query: 1   MKNFALL-SFLFILLASE--VRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGL 60
           MKNF +L  FLFILLAS   +R S ADASP+AV DIDGKKLRAGVNYYILPVFRGRGGGL
Sbjct: 1   MKNFGILFYFLFILLASTQLIRFSTADASPEAVLDIDGKKLRAGVNYYILPVFRGRGGGL 60

Query: 61  ALGNLQSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTV 120
            LGNLQS+KCPLNVVQEQ E+MNG P  F PVNPKKGVVRVSTDLNVQFEASTIC TSTV
Sbjct: 61  TLGNLQSEKCPLNVVQEQLEVMNGFPTTFHPVNPKKGVVRVSTDLNVQFEASTICVTSTV 120

Query: 121 WKLDKFDESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRD 180
           WKLDKFDEST QW +TIGG+RGNPGV+TVDNWFKIEKHG DYK  FCPTVC+FCKVMCRD
Sbjct: 121 WKLDKFDESTGQWLVTIGGSRGNPGVETVDNWFKIEKHGKDYKLVFCPTVCNFCKVMCRD 180

Query: 181 VGIFFKNGKRALALSDTPFPVMFKEV 204
           +GIFFKNG+RALALSDTPFPVMFK+V
Sbjct: 181 IGIFFKNGERALALSDTPFPVMFKKV 206

[Close]

BLAST of Carg04878 vs. TAIR 10
Match: AT1G17860.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 231.5 bits (589), Expect = 6.1e-61
Identity = 108/197 (54.82%), Postives = 144/197 (73.10%), Query Frame = 0


Query: 7   LSFLFILLASEV--RVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLALGNLQS 66
           L ++F+LLA  +  R    +A+ + V+DI+GK L  GVNYYILPV RGRGGGL + NL++
Sbjct: 4   LLYIFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKT 63

Query: 67  DKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTVWKLDKFD 126
           + CP +V+Q+QFE+  GLP  F P + K   + VSTD+N++F      + +++W+L  FD
Sbjct: 64  ETCPTSVIQDQFEVSQGLPVKFSPYD-KSRTIPVSTDVNIKF------SPTSIWELANFD 123

Query: 127 ESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDVGIFFKN 186
           E+TKQWFI+  G  GNPG KTVDNWFKI+K   DYK +FCPTVC+FCKV+CRDVG+F ++
Sbjct: 124 ETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFVQD 183

Query: 187 GKRALALSDTPFPVMFK 202
           GKR LALSD P  VMFK
Sbjct: 184 GKRRLALSDVPLKVMFK 193

[Close]

BLAST of Carg04878 vs. TAIR 10
Match: AT1G73260.1 (kunitz trypsin inhibitor 1 )

HSP 1 Score: 146.4 bits (368), Expect = 2.6e-35
Identity = 85/197 (43.15%), Postives = 109/197 (55.33%), Query Frame = 0


Query: 9   FLFILLASEVRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLALGNLQSDKCP 68
           F  +L  + V  S A     AV DIDG  +    +YY+LPV RGRGGGL L       CP
Sbjct: 12  FYLVLALTAVLASNAYG---AVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAGRGGQPCP 71

Query: 69  LNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFE-ASTICATSTVWKLDKFDEST 128
            ++VQE  E+  G+P  F     K   V  S +LN++ +  +TIC  ST W++ +FD   
Sbjct: 72  YDIVQESSEVDEGIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHER 131

Query: 129 KQWFITIGGTRGNPGVKTVDNWFKIEKHGND-YKFKFCPTVCDFCKVMCRDVGIFFKN-G 188
           KQ+F+  G      G  ++ ++FKIEK G D YKF FCP  CD     C DVGIF    G
Sbjct: 132 KQYFVVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCDSGNPKCSDVGIFIDELG 191

Query: 189 KRALALSDTPFPVMFKE 203
            R LALSD PF VMFK+
Sbjct: 192 VRRLALSDKPFLVMFKK 204

[Close]

BLAST of Carg04878 vs. TAIR 10
Match: AT1G73325.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 104.8 bits (260), Expect = 8.6e-23
Identity = 72/206 (34.95%), Postives = 112/206 (54.37%), Query Frame = 0


Query: 7   LSFLFILLASEV--RVSRADASP-DAVRDIDGKKLRAGVNYYILPVFRGRGGGLALGNLQ 66
           LSF+ + + S +    S ADA+P   V DI G  +++ V YYI+P   G GGGL   N  
Sbjct: 6   LSFITLTVLSAIFTAASAADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLIPSNRN 65

Query: 67  ---SDKC-PLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEAST-ICATSTVW 126
               D C  L++VQ     ++GLP  F P+N K   V++S  LN++F+++  +C  S VW
Sbjct: 66  LSTQDLCLNLDIVQSSSPFVSGLPVTFSPLNTKVKHVQLSASLNLEFDSTVWLCPDSKVW 125

Query: 127 KLDKFDESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRDV 186
           ++D   +  K  F++IGG +G       ++WF+I++ G+ YK  +CP       V C +V
Sbjct: 126 RIDHSVQLRKS-FVSIGGQKGKG-----NSWFQIQEDGDAYKLMYCPI---SSIVACINV 185

Query: 187 GI-FFKNGKRALALS-DTPFPVMFKE 203
            +    +G R L LS D  F V F++
Sbjct: 186 SLEIDDHGVRRLVLSTDQSFVVKFQK 202

The following BLAST results are available for this feature:

BLAST of Carg04878 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022923703.1	2.0e-114	100.00	kunitz trypsin inhibitor 2 [Cucurbita moschata] >XP_023001808.1 kunitz trypsin i...	[more]
XP_023520252.1	4.9e-113	99.01	kunitz trypsin inhibitor 2 [Cucurbita pepo subsp. pepo]	[more]
XP_038895203.1	9.0e-99	87.32	kunitz trypsin inhibitor 5-like [Benincasa hispida]	[more]
XP_008437058.1	3.8e-97	84.73	PREDICTED: miraculin-like [Cucumis melo] >TYJ99073.1 miraculin-like [Cucumis mel...	[more]
XP_004137534.1	9.3e-96	83.98	kunitz trypsin inhibitor 5 [Cucumis sativus]	[more]

BLAST of Carg04878 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LMU2	8.5e-60	54.82	Kunitz trypsin inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=KTI5 PE=2 SV=1	[more]
P13087	6.5e-52	50.00	Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3	[more]
P32765	2.3e-41	43.84	21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1	[more]
Q8RXD5	3.6e-34	43.15	Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1	[more]
P80691	7.5e-24	38.89	Latex serine proteinase inhibitor OS=Carica papaya OX=3649 PE=1 SV=1	[more]

BLAST of Carg04878 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KRM6	9.6e-115	100.00	kunitz trypsin inhibitor 2 OS=Cucurbita maxima OX=3661 GN=LOC111495838 PE=3 SV=1	[more]
A0A6J1EAD6	9.6e-115	100.00	kunitz trypsin inhibitor 2 OS=Cucurbita moschata OX=3662 GN=LOC111431333 PE=3 SV...	[more]
A0A5D3BIG0	1.8e-97	84.73	Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00277...	[more]
A0A1S3ASR3	1.8e-97	84.73	miraculin-like OS=Cucumis melo OX=3656 GN=LOC103482596 PE=3 SV=1	[more]
A0A0A0LTW2	4.5e-96	83.98	Tumor-related protein OS=Cucumis sativus OX=3659 GN=Csa_1G043200 PE=3 SV=1	[more]

BLAST of Carg04878 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G17860.1	6.1e-61	54.82	Kunitz family trypsin and protease inhibitor protein	[more]
AT1G73260.1	2.6e-35	43.15	kunitz trypsin inhibitor 1	[more]
AT1G73325.1	8.6e-23	34.95	Kunitz family trypsin and protease inhibitor protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	PRINTS	PR00291	KUNITZINHBTR	coord: 29..58 score: 43.53 coord: 172..201 score: 25.95 coord: 67..87 score: 31.93 coord: 149..168 score: 25.39
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 29..203 e-value: 3.3E-68 score: 242.6
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 30..201 e-value: 3.4E-57 score: 193.2
IPR002160	Proteinase inhibitor I3, Kunitz legume	PANTHER	PTHR33107	KUNITZ TRYPSIN INHIBITOR 2	coord: 7..202
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 30..46
IPR002160	Proteinase inhibitor I3, Kunitz legume	CDD	cd00178	STI	coord: 29..201 e-value: 6.56568E-56 score: 173.297
None	No IPR available	GENE3D	2.80.10.50		coord: 24..202 e-value: 1.8E-73 score: 247.9
None	No IPR available	PANTHER	PTHR33107:SF5	KUNITZ TRYPSIN INHIBITOR 2	coord: 7..202
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	50386	STI-like	coord: 26..202

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg04878-RA	Carg04878-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004866	endopeptidase inhibitor activity