Carg04789 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg04789
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptioncalcium sensing receptor, chloroplastic
LocationCarg_Chr13: 9287273 .. 9288985 (+)
RNA-Seq ExpressionCarg04789
SyntenyCarg04789
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CAGAGGTAGCGACAATGGCGATGAAGATGGCCATCAGAGCTTCCGCCGTTACCCCACATCATTCACTTCCTTCACTGCCTTCGTCCTCTCAAAGATCCGATTCCAAATCTCAATTCCGTCCCATCTCCGTTGGCTTACCGGCGTCCACTACCCTCTCGATGTTAGCCCTAATTTCCTCTCCCTATGAAGCTAGGGCTCTCAATAAGGACCAAATCGTTTCATCTCTCAATGATGTAAGTATCATTCTTTTGTTCCAGGTAAATGGTCGGTTTTCAACAAAGAATTTCTCACAGTTGTTTAATGCTTTGTGGAATGGTAGGTGGAGAAGACGATTGATCAGGTTCAAGAAGCGGGTTCGAATTTCTTTGATATTGCACAACAGCTCATTGACTCTGCTAGAACTATTTTGAAGCCGGGTGTTGATGCGGCGCTGCCGATTGTAAAGCAGGCTGGAGAAGAGGCGCTGAAGGTTGCTTCTCCTACAATTTCTGAGGCTTCAAAGAAAGCCCTCGAAGCACTTCAGGGCTCTGGCATTGACACCCAGCCTGTTCTAACTGCTGCTAAGGTCTGTGCTTCTTAATTGGACGCTATTAACTCAGTTTAATACATGAAAGAAGATTATGGCTGAGAATCAGTAGACTGTTCAAATCTGGGTCTGCTCTACTCGATGATTTTAGTATTTGTTCGACTGTTTTCATCAAACCCAGCTTCACATATCAAATACTTTCATGGTTGCAGACAGTAGTAGGTGCAGCGGAACAAACTGGGAAGGTCATTGAGGGTGCCAAGCCTATAGCCTCGTCGACTGTCGAGACAATCTCCACAGCAGACCCTCTTGTAATTGCAGAAATTGCTGGAGCCTTAGCCGTGGCATACCTGCTCTTTCCTCCAATTTGGTCGGCCATATCTTTCAGCTTTCGTGGCTACAAAGGTGCATCTGCTCTCTTTTATGGAAATGCCTCTTGATTTGGAGGTTTCGTTTGTACAGAGTGATTTAGTCTGATTGGTGGTATAATTCTGTATCTTGATTCTTGACCAGGTGAGCTCTCTCCTGCTCAAACACTTGAGCTAATTTCGTCGAGCAATTACGTTCTGATCGATATACGATCCGAGAAAGACAAGGATAAGTCTGGCATTCCTCGCCTTCCATCTAGTGCTAAGAACCAGTTGATTGCCATTCCGTAAGTCAAACAAGCTTGCATTTTTCATAAATTCCATATGGGGGCACCTTATCAGCAGCATTTGCATCAAATTGTTTCAGATTGGAAGAATTACCGAGCAAGTTGCGGGGCATTGTCCGAAATGTAAAGAAGCTAGAAGCTGAACTTTCAGCCATCAAGATATCATATCTCAAGAAACTTAACAAAGGCTCCAATGTAGTGATCTTGGGCTCGTGAGTAATCTTGCCTGACTCAAATATTTCTTTGATCTACTTCATTTGTTCATTAAATTTCATGGAAATTCAGGTACTCAGACTCGGGAAAAGCAGTTGCAAAAGCACTGACAAGGCTTGGCTTCAAGAACTCCTGGATTGTAACAGATGGGTTTTTAGGTAGCAAAGGTTGGTTACAGAGTCGGTTAGGGACAGATACATATAAGTTCTCATTTGCAGAGATCCTTTCTCCGTCCCGAGTCATCCCCGCCGCAACCAAACGTTTCGGGACGACGTCACTGACAACATCTGGTCAAAAATTGCTTCCTGGTGCTGATTAA

mRNA sequence

CAGAGGTAGCGACAATGGCGATGAAGATGGCCATCAGAGCTTCCGCCGTTACCCCACATCATTCACTTCCTTCACTGCCTTCGTCCTCTCAAAGATCCGATTCCAAATCTCAATTCCGTCCCATCTCCGTTGGCTTACCGGCGTCCACTACCCTCTCGATGTTAGCCCTAATTTCCTCTCCCTATGAAGCTAGGGCTCTCAATAAGGACCAAATCGTTTCATCTCTCAATGATGTGGAGAAGACGATTGATCAGGTTCAAGAAGCGGGTTCGAATTTCTTTGATATTGCACAACAGCTCATTGACTCTGCTAGAACTATTTTGAAGCCGGGTGTTGATGCGGCGCTGCCGATTGTAAAGCAGGCTGGAGAAGAGGCGCTGAAGGTTGCTTCTCCTACAATTTCTGAGGCTTCAAAGAAAGCCCTCGAAGCACTTCAGGGCTCTGGCATTGACACCCAGCCTGTTCTAACTGCTGCTAAGACAGTAGTAGGTGCAGCGGAACAAACTGGGAAGGTCATTGAGGGTGCCAAGCCTATAGCCTCGTCGACTGTCGAGACAATCTCCACAGCAGACCCTCTTGTAATTGCAGAAATTGCTGGAGCCTTAGCCGTGGCATACCTGCTCTTTCCTCCAATTTGGTCGGCCATATCTTTCAGCTTTCGTGGCTACAAAGGTGAGCTCTCTCCTGCTCAAACACTTGAGCTAATTTCGTCGAGCAATTACGTTCTGATCGATATACGATCCGAGAAAGACAAGGATAAGTCTGGCATTCCTCGCCTTCCATCTAGTGCTAAGAACCAGTTGATTGCCATTCCATTGGAAGAATTACCGAGCAAGTTGCGGGGCATTGTCCGAAATGTAAAGAAGCTAGAAGCTGAACTTTCAGCCATCAAGATATCATATCTCAAGAAACTTAACAAAGGCTCCAATGTAGTGATCTTGGGCTCGTACTCAGACTCGGGAAAAGCAGTTGCAAAAGCACTGACAAGGCTTGGCTTCAAGAACTCCTGGATTGTAACAGATGGGTTTTTAGGTAGCAAAGGTTGGTTACAGAGTCGGTTAGGGACAGATACATATAAGTTCTCATTTGCAGAGATCCTTTCTCCGTCCCGAGTCATCCCCGCCGCAACCAAACGTTTCGGGACGACGTCACTGACAACATCTGGTCAAAAATTGCTTCCTGGTGCTGATTAA

Coding sequence (CDS)

ATGGCGATGAAGATGGCCATCAGAGCTTCCGCCGTTACCCCACATCATTCACTTCCTTCACTGCCTTCGTCCTCTCAAAGATCCGATTCCAAATCTCAATTCCGTCCCATCTCCGTTGGCTTACCGGCGTCCACTACCCTCTCGATGTTAGCCCTAATTTCCTCTCCCTATGAAGCTAGGGCTCTCAATAAGGACCAAATCGTTTCATCTCTCAATGATGTGGAGAAGACGATTGATCAGGTTCAAGAAGCGGGTTCGAATTTCTTTGATATTGCACAACAGCTCATTGACTCTGCTAGAACTATTTTGAAGCCGGGTGTTGATGCGGCGCTGCCGATTGTAAAGCAGGCTGGAGAAGAGGCGCTGAAGGTTGCTTCTCCTACAATTTCTGAGGCTTCAAAGAAAGCCCTCGAAGCACTTCAGGGCTCTGGCATTGACACCCAGCCTGTTCTAACTGCTGCTAAGACAGTAGTAGGTGCAGCGGAACAAACTGGGAAGGTCATTGAGGGTGCCAAGCCTATAGCCTCGTCGACTGTCGAGACAATCTCCACAGCAGACCCTCTTGTAATTGCAGAAATTGCTGGAGCCTTAGCCGTGGCATACCTGCTCTTTCCTCCAATTTGGTCGGCCATATCTTTCAGCTTTCGTGGCTACAAAGGTGAGCTCTCTCCTGCTCAAACACTTGAGCTAATTTCGTCGAGCAATTACGTTCTGATCGATATACGATCCGAGAAAGACAAGGATAAGTCTGGCATTCCTCGCCTTCCATCTAGTGCTAAGAACCAGTTGATTGCCATTCCATTGGAAGAATTACCGAGCAAGTTGCGGGGCATTGTCCGAAATGTAAAGAAGCTAGAAGCTGAACTTTCAGCCATCAAGATATCATATCTCAAGAAACTTAACAAAGGCTCCAATGTAGTGATCTTGGGCTCGTACTCAGACTCGGGAAAAGCAGTTGCAAAAGCACTGACAAGGCTTGGCTTCAAGAACTCCTGGATTGTAACAGATGGGTTTTTAGGTAGCAAAGGTTGGTTACAGAGTCGGTTAGGGACAGATACATATAAGTTCTCATTTGCAGAGATCCTTTCTCCGTCCCGAGTCATCCCCGCCGCAACCAAACGTTTCGGGACGACGTCACTGACAACATCTGGTCAAAAATTGCTTCCTGGTGCTGATTAA

Protein sequence

MAMKMAIRASAVTPHHSLPSLPSSSQRSDSKSQFRPISVGLPASTTLSMLALISSPYEARALNKDQIVSSLNDVEKTIDQVQEAGSNFFDIAQQLIDSARTILKPGVDAALPIVKQAGEEALKVASPTISEASKKALEALQGSGIDTQPVLTAAKTVVGAAEQTGKVIEGAKPIASSTVETISTADPLVIAEIAGALAVAYLLFPPIWSAISFSFRGYKGELSPAQTLELISSSNYVLIDIRSEKDKDKSGIPRLPSSAKNQLIAIPLEELPSKLRGIVRNVKKLEAELSAIKISYLKKLNKGSNVVILGSYSDSGKAVAKALTRLGFKNSWIVTDGFLGSKGWLQSRLGTDTYKFSFAEILSPSRVIPAATKRFGTTSLTTSGQKLLPGAD
Homology
BLAST of Carg04789 vs. NCBI nr
Match: KAG7019969.1 (Calcium sensing receptor, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 723.8 bits (1867), Expect = 8.1e-205
Identity = 392/392 (100.00%), Postives = 392/392 (100.00%), Query Frame = 0

Query: 1   MAMKMAIRASAVTPHHSLPSLPSSSQRSDSKSQFRPISVGLPASTTLSMLALISSPYEAR 60
           MAMKMAIRASAVTPHHSLPSLPSSSQRSDSKSQFRPISVGLPASTTLSMLALISSPYEAR
Sbjct: 1   MAMKMAIRASAVTPHHSLPSLPSSSQRSDSKSQFRPISVGLPASTTLSMLALISSPYEAR 60

Query: 61  ALNKDQIVSSLNDVEKTIDQVQEAGSNFFDIAQQLIDSARTILKPGVDAALPIVKQAGEE 120
           ALNKDQIVSSLNDVEKTIDQVQEAGSNFFDIAQQLIDSARTILKPGVDAALPIVKQAGEE
Sbjct: 61  ALNKDQIVSSLNDVEKTIDQVQEAGSNFFDIAQQLIDSARTILKPGVDAALPIVKQAGEE 120

Query: 121 ALKVASPTISEASKKALEALQGSGIDTQPVLTAAKTVVGAAEQTGKVIEGAKPIASSTVE 180
           ALKVASPTISEASKKALEALQGSGIDTQPVLTAAKTVVGAAEQTGKVIEGAKPIASSTVE
Sbjct: 121 ALKVASPTISEASKKALEALQGSGIDTQPVLTAAKTVVGAAEQTGKVIEGAKPIASSTVE 180

Query: 181 TISTADPLVIAEIAGALAVAYLLFPPIWSAISFSFRGYKGELSPAQTLELISSSNYVLID 240
           TISTADPLVIAEIAGALAVAYLLFPPIWSAISFSFRGYKGELSPAQTLELISSSNYVLID
Sbjct: 181 TISTADPLVIAEIAGALAVAYLLFPPIWSAISFSFRGYKGELSPAQTLELISSSNYVLID 240

Query: 241 IRSEKDKDKSGIPRLPSSAKNQLIAIPLEELPSKLRGIVRNVKKLEAELSAIKISYLKKL 300
           IRSEKDKDKSGIPRLPSSAKNQLIAIPLEELPSKLRGIVRNVKKLEAELSAIKISYLKKL
Sbjct: 241 IRSEKDKDKSGIPRLPSSAKNQLIAIPLEELPSKLRGIVRNVKKLEAELSAIKISYLKKL 300

Query: 301 NKGSNVVILGSYSDSGKAVAKALTRLGFKNSWIVTDGFLGSKGWLQSRLGTDTYKFSFAE 360
           NKGSNVVILGSYSDSGKAVAKALTRLGFKNSWIVTDGFLGSKGWLQSRLGTDTYKFSFAE
Sbjct: 301 NKGSNVVILGSYSDSGKAVAKALTRLGFKNSWIVTDGFLGSKGWLQSRLGTDTYKFSFAE 360

Query: 361 ILSPSRVIPAATKRFGTTSLTTSGQKLLPGAD 393
           ILSPSRVIPAATKRFGTTSLTTSGQKLLPGAD
Sbjct: 361 ILSPSRVIPAATKRFGTTSLTTSGQKLLPGAD 392

BLAST of Carg04789 vs. NCBI nr
Match: KAG6584382.1 (Calcium sensing receptor, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 723.4 bits (1866), Expect = 1.1e-204
Identity = 391/392 (99.74%), Postives = 392/392 (100.00%), Query Frame = 0

Query: 1   MAMKMAIRASAVTPHHSLPSLPSSSQRSDSKSQFRPISVGLPASTTLSMLALISSPYEAR 60
           MAMKMA+RASAVTPHHSLPSLPSSSQRSDSKSQFRPISVGLPASTTLSMLALISSPYEAR
Sbjct: 1   MAMKMAVRASAVTPHHSLPSLPSSSQRSDSKSQFRPISVGLPASTTLSMLALISSPYEAR 60

Query: 61  ALNKDQIVSSLNDVEKTIDQVQEAGSNFFDIAQQLIDSARTILKPGVDAALPIVKQAGEE 120
           ALNKDQIVSSLNDVEKTIDQVQEAGSNFFDIAQQLIDSARTILKPGVDAALPIVKQAGEE
Sbjct: 61  ALNKDQIVSSLNDVEKTIDQVQEAGSNFFDIAQQLIDSARTILKPGVDAALPIVKQAGEE 120

Query: 121 ALKVASPTISEASKKALEALQGSGIDTQPVLTAAKTVVGAAEQTGKVIEGAKPIASSTVE 180
           ALKVASPTISEASKKALEALQGSGIDTQPVLTAAKTVVGAAEQTGKVIEGAKPIASSTVE
Sbjct: 121 ALKVASPTISEASKKALEALQGSGIDTQPVLTAAKTVVGAAEQTGKVIEGAKPIASSTVE 180

Query: 181 TISTADPLVIAEIAGALAVAYLLFPPIWSAISFSFRGYKGELSPAQTLELISSSNYVLID 240
           TISTADPLVIAEIAGALAVAYLLFPPIWSAISFSFRGYKGELSPAQTLELISSSNYVLID
Sbjct: 181 TISTADPLVIAEIAGALAVAYLLFPPIWSAISFSFRGYKGELSPAQTLELISSSNYVLID 240

Query: 241 IRSEKDKDKSGIPRLPSSAKNQLIAIPLEELPSKLRGIVRNVKKLEAELSAIKISYLKKL 300
           IRSEKDKDKSGIPRLPSSAKNQLIAIPLEELPSKLRGIVRNVKKLEAELSAIKISYLKKL
Sbjct: 241 IRSEKDKDKSGIPRLPSSAKNQLIAIPLEELPSKLRGIVRNVKKLEAELSAIKISYLKKL 300

Query: 301 NKGSNVVILGSYSDSGKAVAKALTRLGFKNSWIVTDGFLGSKGWLQSRLGTDTYKFSFAE 360
           NKGSNVVILGSYSDSGKAVAKALTRLGFKNSWIVTDGFLGSKGWLQSRLGTDTYKFSFAE
Sbjct: 301 NKGSNVVILGSYSDSGKAVAKALTRLGFKNSWIVTDGFLGSKGWLQSRLGTDTYKFSFAE 360

Query: 361 ILSPSRVIPAATKRFGTTSLTTSGQKLLPGAD 393
           ILSPSRVIPAATKRFGTTSLTTSGQKLLPGAD
Sbjct: 361 ILSPSRVIPAATKRFGTTSLTTSGQKLLPGAD 392

BLAST of Carg04789 vs. NCBI nr
Match: XP_022924156.1 (calcium sensing receptor, chloroplastic [Cucurbita moschata])

HSP 1 Score: 720.3 bits (1858), Expect = 8.9e-204
Identity = 390/392 (99.49%), Postives = 391/392 (99.74%), Query Frame = 0

Query: 1   MAMKMAIRASAVTPHHSLPSLPSSSQRSDSKSQFRPISVGLPASTTLSMLALISSPYEAR 60
           MAMKMA+RASAVTPH SLPSLPSSSQRSDSKSQFRPISVGLPASTTLSMLALISSPYEAR
Sbjct: 1   MAMKMAVRASAVTPHRSLPSLPSSSQRSDSKSQFRPISVGLPASTTLSMLALISSPYEAR 60

Query: 61  ALNKDQIVSSLNDVEKTIDQVQEAGSNFFDIAQQLIDSARTILKPGVDAALPIVKQAGEE 120
           ALNKDQIVSSLNDVEKTIDQVQEAGSNFFDIAQQLIDSARTILKPGVDAALPIVKQAGEE
Sbjct: 61  ALNKDQIVSSLNDVEKTIDQVQEAGSNFFDIAQQLIDSARTILKPGVDAALPIVKQAGEE 120

Query: 121 ALKVASPTISEASKKALEALQGSGIDTQPVLTAAKTVVGAAEQTGKVIEGAKPIASSTVE 180
           ALKVASPTISEASKKALEALQGSGIDTQPVLTAAKTVVGAAEQTGKVIEGAKPIASSTVE
Sbjct: 121 ALKVASPTISEASKKALEALQGSGIDTQPVLTAAKTVVGAAEQTGKVIEGAKPIASSTVE 180

Query: 181 TISTADPLVIAEIAGALAVAYLLFPPIWSAISFSFRGYKGELSPAQTLELISSSNYVLID 240
           TISTADPLVIAEIAGALAVAYLLFPPIWSAISFSFRGYKGELSPAQTLELISSSNYVLID
Sbjct: 181 TISTADPLVIAEIAGALAVAYLLFPPIWSAISFSFRGYKGELSPAQTLELISSSNYVLID 240

Query: 241 IRSEKDKDKSGIPRLPSSAKNQLIAIPLEELPSKLRGIVRNVKKLEAELSAIKISYLKKL 300
           IRSEKDKDKSGIPRLPSSAKNQLIAIPLEELPSKLRGIVRNVKKLEAELSAIKISYLKKL
Sbjct: 241 IRSEKDKDKSGIPRLPSSAKNQLIAIPLEELPSKLRGIVRNVKKLEAELSAIKISYLKKL 300

Query: 301 NKGSNVVILGSYSDSGKAVAKALTRLGFKNSWIVTDGFLGSKGWLQSRLGTDTYKFSFAE 360
           NKGSNVVILGSYSDSGKAVAKALTRLGFKNSWIVTDGFLGSKGWLQSRLGTDTYKFSFAE
Sbjct: 301 NKGSNVVILGSYSDSGKAVAKALTRLGFKNSWIVTDGFLGSKGWLQSRLGTDTYKFSFAE 360

Query: 361 ILSPSRVIPAATKRFGTTSLTTSGQKLLPGAD 393
           ILSPSRVIPAATKRFGTTSLTTSGQKLLPGAD
Sbjct: 361 ILSPSRVIPAATKRFGTTSLTTSGQKLLPGAD 392

BLAST of Carg04789 vs. NCBI nr
Match: XP_023000782.1 (calcium sensing receptor, chloroplastic [Cucurbita maxima])

HSP 1 Score: 715.3 bits (1845), Expect = 2.9e-202
Identity = 386/392 (98.47%), Postives = 389/392 (99.23%), Query Frame = 0

Query: 1   MAMKMAIRASAVTPHHSLPSLPSSSQRSDSKSQFRPISVGLPASTTLSMLALISSPYEAR 60
           MAMKMA+RASAVTPHHSLPSLPSSS RSDSKSQFRPISVGLPASTTLSMLALISSPYEAR
Sbjct: 1   MAMKMAVRASAVTPHHSLPSLPSSSHRSDSKSQFRPISVGLPASTTLSMLALISSPYEAR 60

Query: 61  ALNKDQIVSSLNDVEKTIDQVQEAGSNFFDIAQQLIDSARTILKPGVDAALPIVKQAGEE 120
           ALNKD IVSSLNDVEKTIDQVQEAGSNFFDIAQQ+IDSARTILKPGVDAALPIVKQAGEE
Sbjct: 61  ALNKDLIVSSLNDVEKTIDQVQEAGSNFFDIAQQVIDSARTILKPGVDAALPIVKQAGEE 120

Query: 121 ALKVASPTISEASKKALEALQGSGIDTQPVLTAAKTVVGAAEQTGKVIEGAKPIASSTVE 180
           ALKVASPTISEASKKALEALQGSGIDTQPVLTAAKTVVGAAEQTGKVIEGAKPIASSTVE
Sbjct: 121 ALKVASPTISEASKKALEALQGSGIDTQPVLTAAKTVVGAAEQTGKVIEGAKPIASSTVE 180

Query: 181 TISTADPLVIAEIAGALAVAYLLFPPIWSAISFSFRGYKGELSPAQTLELISSSNYVLID 240
           TISTADPLVIAEIAGALAVAYLLFPPIWSAISFSFRGYKGELSPAQTLELISSSNYVLID
Sbjct: 181 TISTADPLVIAEIAGALAVAYLLFPPIWSAISFSFRGYKGELSPAQTLELISSSNYVLID 240

Query: 241 IRSEKDKDKSGIPRLPSSAKNQLIAIPLEELPSKLRGIVRNVKKLEAELSAIKISYLKKL 300
           IRSEKDKDKSGIPRLPSSAKNQLIAIPLEELPSKLRGIVRNVKKLEAELSAIKISYLKKL
Sbjct: 241 IRSEKDKDKSGIPRLPSSAKNQLIAIPLEELPSKLRGIVRNVKKLEAELSAIKISYLKKL 300

Query: 301 NKGSNVVILGSYSDSGKAVAKALTRLGFKNSWIVTDGFLGSKGWLQSRLGTDTYKFSFAE 360
           NKGSN+VILGSYSDSGKAVAKALTRLGFKNSWIVTDGFLGSKGWLQSRLGTDTYKFSFAE
Sbjct: 301 NKGSNIVILGSYSDSGKAVAKALTRLGFKNSWIVTDGFLGSKGWLQSRLGTDTYKFSFAE 360

Query: 361 ILSPSRVIPAATKRFGTTSLTTSGQKLLPGAD 393
           ILSPSRVIPAATKRFGTTSLT SGQKLLPGAD
Sbjct: 361 ILSPSRVIPAATKRFGTTSLTASGQKLLPGAD 392

BLAST of Carg04789 vs. NCBI nr
Match: XP_023519333.1 (calcium sensing receptor, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 714.9 bits (1844), Expect = 3.7e-202
Identity = 385/392 (98.21%), Postives = 389/392 (99.23%), Query Frame = 0

Query: 1   MAMKMAIRASAVTPHHSLPSLPSSSQRSDSKSQFRPISVGLPASTTLSMLALISSPYEAR 60
           MAMKMA+RASAVTPHHSLPSLPSSS RSDSKSQFRPISVGLPASTTLSMLALISSPYEAR
Sbjct: 1   MAMKMAVRASAVTPHHSLPSLPSSSHRSDSKSQFRPISVGLPASTTLSMLALISSPYEAR 60

Query: 61  ALNKDQIVSSLNDVEKTIDQVQEAGSNFFDIAQQLIDSARTILKPGVDAALPIVKQAGEE 120
           ALNKDQIVSSLNDVEKTIDQVQEAGSNFFDIAQQLIDSARTILKPGVDAALPIVKQAGEE
Sbjct: 61  ALNKDQIVSSLNDVEKTIDQVQEAGSNFFDIAQQLIDSARTILKPGVDAALPIVKQAGEE 120

Query: 121 ALKVASPTISEASKKALEALQGSGIDTQPVLTAAKTVVGAAEQTGKVIEGAKPIASSTVE 180
           ALKVASPTISEASKKALEALQGSGIDTQPVLTAA+TVVGAA+QTGKVIEGAKPIASSTVE
Sbjct: 121 ALKVASPTISEASKKALEALQGSGIDTQPVLTAAQTVVGAAQQTGKVIEGAKPIASSTVE 180

Query: 181 TISTADPLVIAEIAGALAVAYLLFPPIWSAISFSFRGYKGELSPAQTLELISSSNYVLID 240
           TISTADPLVIAEIAG LAVAYLLFPPIWSAISFSFRGYKGELSPAQTLELISSSNYVLID
Sbjct: 181 TISTADPLVIAEIAGVLAVAYLLFPPIWSAISFSFRGYKGELSPAQTLELISSSNYVLID 240

Query: 241 IRSEKDKDKSGIPRLPSSAKNQLIAIPLEELPSKLRGIVRNVKKLEAELSAIKISYLKKL 300
           IRSEKDKDKSGIPRLPSSAKNQLIAIPLEELPSKLRGIVRNVKKLEAELSAIKISYLKKL
Sbjct: 241 IRSEKDKDKSGIPRLPSSAKNQLIAIPLEELPSKLRGIVRNVKKLEAELSAIKISYLKKL 300

Query: 301 NKGSNVVILGSYSDSGKAVAKALTRLGFKNSWIVTDGFLGSKGWLQSRLGTDTYKFSFAE 360
           NKGSN+VILGSYSDSGKAVAKALTRLGFKNSWIVTDGFLGSKGWLQSRLGTDTYKFSFAE
Sbjct: 301 NKGSNIVILGSYSDSGKAVAKALTRLGFKNSWIVTDGFLGSKGWLQSRLGTDTYKFSFAE 360

Query: 361 ILSPSRVIPAATKRFGTTSLTTSGQKLLPGAD 393
           ILSPSRVIPAATKRFGTTSLT SGQKLLPGAD
Sbjct: 361 ILSPSRVIPAATKRFGTTSLTASGQKLLPGAD 392

BLAST of Carg04789 vs. ExPASy Swiss-Prot
Match: Q9FN48 (Calcium sensing receptor, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CAS PE=1 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 5.6e-124
Identity = 242/396 (61.11%), Postives = 314/396 (79.29%), Query Frame = 0

Query: 1   MAMKMAIRASAVTPHHSLPSLPSSSQRSDSKSQFRPISVGLPASTTLSMLALISS-PYEA 60
           MAM      S+++   +LPS  SS++++ S    R +SV LP ST++S+L+L +S P+EA
Sbjct: 1   MAMAEMATKSSLSAKLTLPS--SSTKKTLS---LRQVSVSLPTSTSISLLSLFASPPHEA 60

Query: 61  RA---LNKDQIVSSLNDVEKTIDQVQEAGSNFFDIAQQLIDSARTILKPGVDAALPIVKQ 120
           +A   + KDQIVSSL +VEKTI+QVQE GS+ FD  Q++       LKP +D ALPI KQ
Sbjct: 61  KAAVSIPKDQIVSSLTEVEKTINQVQETGSSVFDATQRVFQVVGDALKPALDTALPIAKQ 120

Query: 121 AGEEALKVASPTISEASKKALEALQGSGIDTQPVLTAAKTVVGAAEQTGKVIEGAKPIAS 180
           AGEEA+K+ASP  SEASKKA EA+Q SG D++PV  AAKTV   A+QT K IE AKPIAS
Sbjct: 121 AGEEAMKLASPAFSEASKKAQEAMQSSGFDSEPVFNAAKTVTDVAQQTSKAIEDAKPIAS 180

Query: 181 STVETISTADPLVIAEIAGALAVAYLLFPPIWSAISFSFRGYKGELSPAQTLELISSSNY 240
           ST++TIS+ADP VI   AGA  +AYLL PP++SAISF+FRGYKG+L+PAQTL+L+ + NY
Sbjct: 181 STMDTISSADPSVIVVAAGAAFLAYLLLPPVFSAISFNFRGYKGDLTPAQTLDLLCTKNY 240

Query: 241 VLIDIRSEKDKDKSGIPRLPSSAKNQLIAIPLEELPSKLRGIVRNVKKLEAELSAIKISY 300
           +++DIRSEKDK+K+GIPRLPS+AKN++I+IPLEELP+K++GIVRN K++EAE++A+KISY
Sbjct: 241 LMVDIRSEKDKEKAGIPRLPSNAKNRVISIPLEELPNKVKGIVRNSKRVEAEIAALKISY 300

Query: 301 LKKLNKGSNVVILGSYSDSGKAVAKALTRLGFKNSWIVTDGFLGSKGWLQSRLGTDTYKF 360
           LKK+NKGSN++IL SY+DS K VAK L  LG+KN +IVTDGF G +GWLQSRLGTD+Y F
Sbjct: 301 LKKINKGSNIIILDSYTDSAKIVAKTLKVLGYKNCYIVTDGFSGGRGWLQSRLGTDSYNF 360

Query: 361 SFAEILSPSRVIPAATKRFGTTSLTTSGQKLLPGAD 393
           SFA++LSPSR+IPAA++ FG    T SG K LP +D
Sbjct: 361 SFAQVLSPSRIIPAASRSFG----TRSGTKFLPSSD 387

BLAST of Carg04789 vs. ExPASy Swiss-Prot
Match: B6TVL4 (Calcium sensing receptor, chloroplastic OS=Zea mays OX=4577 PE=1 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 3.4e-89
Identity = 186/384 (48.44%), Postives = 260/384 (67.71%), Query Frame = 0

Query: 22  PSSSQRSDSKSQFR--PISVGLPASTTL---SMLALISSPYEARALNKDQIVSSLNDVEK 81
           P++  ++ S+S  R  P      A++++   + L  ++    A AL+K+ +  SL     
Sbjct: 16  PAAPPKTTSRSWERRAPADAAFAAASSVAGSAALLTLTPAAPAAALSKEDVAGSLTKAVD 75

Query: 82  TIDQVQEAGSNFFDIAQQLIDSARTILKPGVDAALPIVKQAGEEALKVASPTISEASKKA 141
           T+ Q  + G      A + + +    L   V  ALP++K A +EALK+A+P +S ASK+A
Sbjct: 76  TVSQAIDVGGK----AAEQVAAVLKALGEAVKPALPVLKSASDEALKLAAPVVSAASKQA 135

Query: 142 LEALQGSGIDTQPVLTAAKTVVGAAEQTGKVIEGAKPIASSTVETISTADPLVIAEIAGA 201
            EALQG+G+D  PVL+ AKT   AAEQ+ KVI+ AKP+AS+ VETI++  P      AG 
Sbjct: 136 TEALQGAGVDPAPVLSVAKT---AAEQSTKVIDAAKPVASAAVETITSLGPEDYVVAAGX 195

Query: 202 LAVAYLLFPPIWSAISFSFRGYKGELSPAQTLELISSSNYVLIDIRSEKDKDKSGIPRLP 261
             +AYLL PP+WS +S S RGYKG+L+PAQ L+ +++  YVLID+RSEKDK K+G+P+LP
Sbjct: 196 AFLAYLLVPPVWSLVSSSLRGYKGDLTPAQALDKVTTQGYVLIDVRSEKDKAKAGLPQLP 255

Query: 262 SSAKNQLIAIPLEELPSKLRGIVRNVKKLEAELSAIKISYLKKLNKGSNVVILGSYSDSG 321
           S+AKN+L+++PLE+LPSKL+G+VRN KK EAE++A+KISYLKK+ KGSNV+I+ SYSD  
Sbjct: 256 SNAKNKLVSVPLEDLPSKLKGMVRNAKKAEAEIAALKISYLKKIGKGSNVIIMDSYSDVA 315

Query: 322 KAVAKALTRLGFKNSWIVTDGFLGSKGWLQSRLGTDTYKFSFAEILSPSRVIPA-ATKRF 381
           K VAK L  +GFKN W++  GF G KGW QSRLGTD+Y  S  E+++PSRVIPA A +R 
Sbjct: 316 KTVAKTLDSVGFKNCWVMAGGFSGRKGWAQSRLGTDSYNLSVVEVVTPSRVIPAVAGRRT 375

Query: 382 GTTSL---------TTSGQKLLPG 391
           GTT+            + +KLLPG
Sbjct: 376 GTTAARIGTASSASRATTRKLLPG 392

BLAST of Carg04789 vs. ExPASy TrEMBL
Match: A0A6J1E8T4 (calcium sensing receptor, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111431685 PE=4 SV=1)

HSP 1 Score: 720.3 bits (1858), Expect = 4.3e-204
Identity = 390/392 (99.49%), Postives = 391/392 (99.74%), Query Frame = 0

Query: 1   MAMKMAIRASAVTPHHSLPSLPSSSQRSDSKSQFRPISVGLPASTTLSMLALISSPYEAR 60
           MAMKMA+RASAVTPH SLPSLPSSSQRSDSKSQFRPISVGLPASTTLSMLALISSPYEAR
Sbjct: 1   MAMKMAVRASAVTPHRSLPSLPSSSQRSDSKSQFRPISVGLPASTTLSMLALISSPYEAR 60

Query: 61  ALNKDQIVSSLNDVEKTIDQVQEAGSNFFDIAQQLIDSARTILKPGVDAALPIVKQAGEE 120
           ALNKDQIVSSLNDVEKTIDQVQEAGSNFFDIAQQLIDSARTILKPGVDAALPIVKQAGEE
Sbjct: 61  ALNKDQIVSSLNDVEKTIDQVQEAGSNFFDIAQQLIDSARTILKPGVDAALPIVKQAGEE 120

Query: 121 ALKVASPTISEASKKALEALQGSGIDTQPVLTAAKTVVGAAEQTGKVIEGAKPIASSTVE 180
           ALKVASPTISEASKKALEALQGSGIDTQPVLTAAKTVVGAAEQTGKVIEGAKPIASSTVE
Sbjct: 121 ALKVASPTISEASKKALEALQGSGIDTQPVLTAAKTVVGAAEQTGKVIEGAKPIASSTVE 180

Query: 181 TISTADPLVIAEIAGALAVAYLLFPPIWSAISFSFRGYKGELSPAQTLELISSSNYVLID 240
           TISTADPLVIAEIAGALAVAYLLFPPIWSAISFSFRGYKGELSPAQTLELISSSNYVLID
Sbjct: 181 TISTADPLVIAEIAGALAVAYLLFPPIWSAISFSFRGYKGELSPAQTLELISSSNYVLID 240

Query: 241 IRSEKDKDKSGIPRLPSSAKNQLIAIPLEELPSKLRGIVRNVKKLEAELSAIKISYLKKL 300
           IRSEKDKDKSGIPRLPSSAKNQLIAIPLEELPSKLRGIVRNVKKLEAELSAIKISYLKKL
Sbjct: 241 IRSEKDKDKSGIPRLPSSAKNQLIAIPLEELPSKLRGIVRNVKKLEAELSAIKISYLKKL 300

Query: 301 NKGSNVVILGSYSDSGKAVAKALTRLGFKNSWIVTDGFLGSKGWLQSRLGTDTYKFSFAE 360
           NKGSNVVILGSYSDSGKAVAKALTRLGFKNSWIVTDGFLGSKGWLQSRLGTDTYKFSFAE
Sbjct: 301 NKGSNVVILGSYSDSGKAVAKALTRLGFKNSWIVTDGFLGSKGWLQSRLGTDTYKFSFAE 360

Query: 361 ILSPSRVIPAATKRFGTTSLTTSGQKLLPGAD 393
           ILSPSRVIPAATKRFGTTSLTTSGQKLLPGAD
Sbjct: 361 ILSPSRVIPAATKRFGTTSLTTSGQKLLPGAD 392

BLAST of Carg04789 vs. ExPASy TrEMBL
Match: A0A6J1KEL8 (calcium sensing receptor, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111495129 PE=4 SV=1)

HSP 1 Score: 715.3 bits (1845), Expect = 1.4e-202
Identity = 386/392 (98.47%), Postives = 389/392 (99.23%), Query Frame = 0

Query: 1   MAMKMAIRASAVTPHHSLPSLPSSSQRSDSKSQFRPISVGLPASTTLSMLALISSPYEAR 60
           MAMKMA+RASAVTPHHSLPSLPSSS RSDSKSQFRPISVGLPASTTLSMLALISSPYEAR
Sbjct: 1   MAMKMAVRASAVTPHHSLPSLPSSSHRSDSKSQFRPISVGLPASTTLSMLALISSPYEAR 60

Query: 61  ALNKDQIVSSLNDVEKTIDQVQEAGSNFFDIAQQLIDSARTILKPGVDAALPIVKQAGEE 120
           ALNKD IVSSLNDVEKTIDQVQEAGSNFFDIAQQ+IDSARTILKPGVDAALPIVKQAGEE
Sbjct: 61  ALNKDLIVSSLNDVEKTIDQVQEAGSNFFDIAQQVIDSARTILKPGVDAALPIVKQAGEE 120

Query: 121 ALKVASPTISEASKKALEALQGSGIDTQPVLTAAKTVVGAAEQTGKVIEGAKPIASSTVE 180
           ALKVASPTISEASKKALEALQGSGIDTQPVLTAAKTVVGAAEQTGKVIEGAKPIASSTVE
Sbjct: 121 ALKVASPTISEASKKALEALQGSGIDTQPVLTAAKTVVGAAEQTGKVIEGAKPIASSTVE 180

Query: 181 TISTADPLVIAEIAGALAVAYLLFPPIWSAISFSFRGYKGELSPAQTLELISSSNYVLID 240
           TISTADPLVIAEIAGALAVAYLLFPPIWSAISFSFRGYKGELSPAQTLELISSSNYVLID
Sbjct: 181 TISTADPLVIAEIAGALAVAYLLFPPIWSAISFSFRGYKGELSPAQTLELISSSNYVLID 240

Query: 241 IRSEKDKDKSGIPRLPSSAKNQLIAIPLEELPSKLRGIVRNVKKLEAELSAIKISYLKKL 300
           IRSEKDKDKSGIPRLPSSAKNQLIAIPLEELPSKLRGIVRNVKKLEAELSAIKISYLKKL
Sbjct: 241 IRSEKDKDKSGIPRLPSSAKNQLIAIPLEELPSKLRGIVRNVKKLEAELSAIKISYLKKL 300

Query: 301 NKGSNVVILGSYSDSGKAVAKALTRLGFKNSWIVTDGFLGSKGWLQSRLGTDTYKFSFAE 360
           NKGSN+VILGSYSDSGKAVAKALTRLGFKNSWIVTDGFLGSKGWLQSRLGTDTYKFSFAE
Sbjct: 301 NKGSNIVILGSYSDSGKAVAKALTRLGFKNSWIVTDGFLGSKGWLQSRLGTDTYKFSFAE 360

Query: 361 ILSPSRVIPAATKRFGTTSLTTSGQKLLPGAD 393
           ILSPSRVIPAATKRFGTTSLT SGQKLLPGAD
Sbjct: 361 ILSPSRVIPAATKRFGTTSLTASGQKLLPGAD 392

BLAST of Carg04789 vs. ExPASy TrEMBL
Match: A0A5D3BLC0 (Calcium sensing receptor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G001710 PE=4 SV=1)

HSP 1 Score: 645.2 bits (1663), Expect = 1.8e-181
Identity = 350/394 (88.83%), Postives = 368/394 (93.40%), Query Frame = 0

Query: 1   MAMKMAIRASAV-TPHHSLPSLPSSSQRSDSKSQFRPISVGLPASTTLSMLALISSPYEA 60
           MAMK+ IRAS++   HH  PSLPS S RSDSKSQFRPISV LPASTTLS+LALISSPYEA
Sbjct: 1   MAMKIPIRASSIPRHHHPTPSLPSPSHRSDSKSQFRPISVSLPASTTLSLLALISSPYEA 60

Query: 61  RALNKDQIVSSLNDVEKTIDQVQEAGSNFFDIAQQLIDSARTILKPGVDAALPIVKQAGE 120
           RALNKDQIVSSLN+VEKT DQVQE GSNFFDIAQQ I+SA+ +LKPGVDAALPIVKQAGE
Sbjct: 61  RALNKDQIVSSLNEVEKTFDQVQEMGSNFFDIAQQAIESAKNVLKPGVDAALPIVKQAGE 120

Query: 121 EALKVASPTISEASKKALEALQGSGIDTQPVLTAAKTVVGAAEQTGKVIEGAKPIASSTV 180
           EALKVASPTISEASKKALEALQ SGIDT+PVL+AAKTVVGAA+QTGKVIEGAKPIASSTV
Sbjct: 121 EALKVASPTISEASKKALEALQDSGIDTEPVLSAAKTVVGAAQQTGKVIEGAKPIASSTV 180

Query: 181 ETISTADPLVIAEIAGALAVAYLLFPPIWSAISFSFRGYKGELSPAQTLELISSSNYVLI 240
           ETIST DPLVIAEIAG L +AYLLFPPIWSAISF+FRGYKGELSPAQTL+LISSSNY LI
Sbjct: 181 ETISTTDPLVIAEIAGVLVLAYLLFPPIWSAISFNFRGYKGELSPAQTLDLISSSNYFLI 240

Query: 241 DIRSEKDKDKSGIPRLPSSAKNQLIAIPLEELPSKLRGIVRNVKKLEAELSAIKISYLKK 300
           DIRSEKDKDKSGIPRLPSSAKNQ IAIPLEELP+KLRGIVRN KKLEAELSAIKISYLKK
Sbjct: 241 DIRSEKDKDKSGIPRLPSSAKNQSIAIPLEELPNKLRGIVRNAKKLEAELSAIKISYLKK 300

Query: 301 LNKGSNVVILGSYSDSGKAVAKALTRLGFKNSWIVTDGFLGSKGWLQSRLGTDTYKFSFA 360
           LNKGSN+VILGSYSDS KAVAKALT LGFKNSWIVTDGFLGSKGWLQSRLGTDTYKFSFA
Sbjct: 301 LNKGSNIVILGSYSDSAKAVAKALTSLGFKNSWIVTDGFLGSKGWLQSRLGTDTYKFSFA 360

Query: 361 EILSPSRVIPAATKRFGTTSLTT-SGQKLLPGAD 393
           EILSPSRVI + TKRFGTTSLT+ SGQKLLPG D
Sbjct: 361 EILSPSRVISSGTKRFGTTSLTSASGQKLLPGTD 394

BLAST of Carg04789 vs. ExPASy TrEMBL
Match: A0A1S3C0H3 (calcium sensing receptor, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103495398 PE=4 SV=1)

HSP 1 Score: 645.2 bits (1663), Expect = 1.8e-181
Identity = 350/394 (88.83%), Postives = 368/394 (93.40%), Query Frame = 0

Query: 1   MAMKMAIRASAV-TPHHSLPSLPSSSQRSDSKSQFRPISVGLPASTTLSMLALISSPYEA 60
           MAMK+ IRAS++   HH  PSLPS S RSDSKSQFRPISV LPASTTLS+LALISSPYEA
Sbjct: 1   MAMKIPIRASSIPRHHHPTPSLPSPSHRSDSKSQFRPISVSLPASTTLSLLALISSPYEA 60

Query: 61  RALNKDQIVSSLNDVEKTIDQVQEAGSNFFDIAQQLIDSARTILKPGVDAALPIVKQAGE 120
           RALNKDQIVSSLN+VEKT DQVQE GSNFFDIAQQ I+SA+ +LKPGVDAALPIVKQAGE
Sbjct: 61  RALNKDQIVSSLNEVEKTFDQVQEMGSNFFDIAQQAIESAKNVLKPGVDAALPIVKQAGE 120

Query: 121 EALKVASPTISEASKKALEALQGSGIDTQPVLTAAKTVVGAAEQTGKVIEGAKPIASSTV 180
           EALKVASPTISEASKKALEALQ SGIDT+PVL+AAKTVVGAA+QTGKVIEGAKPIASSTV
Sbjct: 121 EALKVASPTISEASKKALEALQDSGIDTEPVLSAAKTVVGAAQQTGKVIEGAKPIASSTV 180

Query: 181 ETISTADPLVIAEIAGALAVAYLLFPPIWSAISFSFRGYKGELSPAQTLELISSSNYVLI 240
           ETIST DPLVIAEIAG L +AYLLFPPIWSAISF+FRGYKGELSPAQTL+LISSSNY LI
Sbjct: 181 ETISTTDPLVIAEIAGVLVLAYLLFPPIWSAISFNFRGYKGELSPAQTLDLISSSNYFLI 240

Query: 241 DIRSEKDKDKSGIPRLPSSAKNQLIAIPLEELPSKLRGIVRNVKKLEAELSAIKISYLKK 300
           DIRSEKDKDKSGIPRLPSSAKNQ IAIPLEELP+KLRGIVRN KKLEAELSAIKISYLKK
Sbjct: 241 DIRSEKDKDKSGIPRLPSSAKNQSIAIPLEELPNKLRGIVRNAKKLEAELSAIKISYLKK 300

Query: 301 LNKGSNVVILGSYSDSGKAVAKALTRLGFKNSWIVTDGFLGSKGWLQSRLGTDTYKFSFA 360
           LNKGSN+VILGSYSDS KAVAKALT LGFKNSWIVTDGFLGSKGWLQSRLGTDTYKFSFA
Sbjct: 301 LNKGSNIVILGSYSDSAKAVAKALTSLGFKNSWIVTDGFLGSKGWLQSRLGTDTYKFSFA 360

Query: 361 EILSPSRVIPAATKRFGTTSLTT-SGQKLLPGAD 393
           EILSPSRVI + TKRFGTTSLT+ SGQKLLPG D
Sbjct: 361 EILSPSRVISSGTKRFGTTSLTSASGQKLLPGTD 394

BLAST of Carg04789 vs. ExPASy TrEMBL
Match: A0A0A0LSX7 (Rhodanese domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G041560 PE=4 SV=1)

HSP 1 Score: 642.5 bits (1656), Expect = 1.1e-180
Identity = 349/394 (88.58%), Postives = 368/394 (93.40%), Query Frame = 0

Query: 1   MAMKMAIRASAV-TPHHSLPSLPSSSQRSDSKSQFRPISVGLPASTTLSMLALISSPYEA 60
           MAM + IRAS++   HH  PSLPS S RSDSKSQFRPISV LPASTTLS+LAL SSPYEA
Sbjct: 1   MAMHIHIRASSIPRHHHPTPSLPSPSHRSDSKSQFRPISVSLPASTTLSLLALFSSPYEA 60

Query: 61  RALNKDQIVSSLNDVEKTIDQVQEAGSNFFDIAQQLIDSARTILKPGVDAALPIVKQAGE 120
           +ALNKDQIVSSLN+VEKT DQVQE GSNFFDIAQQ I+SA+T+LKPGVDAALPIVKQAGE
Sbjct: 61  KALNKDQIVSSLNEVEKTFDQVQEMGSNFFDIAQQAIESAKTVLKPGVDAALPIVKQAGE 120

Query: 121 EALKVASPTISEASKKALEALQGSGIDTQPVLTAAKTVVGAAEQTGKVIEGAKPIASSTV 180
           EALKVASPTISEASKKALEALQ SGIDT+PVL+AAKTVVGAA+QTGKVIEGAKPIASSTV
Sbjct: 121 EALKVASPTISEASKKALEALQDSGIDTEPVLSAAKTVVGAAQQTGKVIEGAKPIASSTV 180

Query: 181 ETISTADPLVIAEIAGALAVAYLLFPPIWSAISFSFRGYKGELSPAQTLELISSSNYVLI 240
           ETIST DPLVIAEIAG LA+AYLLFPPIWSAISF+FRGYKGELSPAQTL+LISSSNY LI
Sbjct: 181 ETISTTDPLVIAEIAGVLALAYLLFPPIWSAISFNFRGYKGELSPAQTLDLISSSNYFLI 240

Query: 241 DIRSEKDKDKSGIPRLPSSAKNQLIAIPLEELPSKLRGIVRNVKKLEAELSAIKISYLKK 300
           DIRSEKDKDKSGIPRLPSSAKNQ IAIP E+LP+KLRGIVRNVKKLEAELSAIKISYLKK
Sbjct: 241 DIRSEKDKDKSGIPRLPSSAKNQAIAIPSEDLPNKLRGIVRNVKKLEAELSAIKISYLKK 300

Query: 301 LNKGSNVVILGSYSDSGKAVAKALTRLGFKNSWIVTDGFLGSKGWLQSRLGTDTYKFSFA 360
           LNKGSN+VILGSYSDS KAVAKALT LGFKNSWIVTDGFLGSKGWLQSRLGTDTYKFSFA
Sbjct: 301 LNKGSNIVILGSYSDSAKAVAKALTSLGFKNSWIVTDGFLGSKGWLQSRLGTDTYKFSFA 360

Query: 361 EILSPSRVIPAATKRFGTTSLT-TSGQKLLPGAD 393
           EILSPSRVI + TKRFGTTSLT TS QKLLPGAD
Sbjct: 361 EILSPSRVISSGTKRFGTTSLTSTSSQKLLPGAD 394

BLAST of Carg04789 vs. TAIR 10
Match: AT5G23060.1 (calcium sensing receptor )

HSP 1 Score: 445.7 bits (1145), Expect = 3.9e-125
Identity = 242/396 (61.11%), Postives = 314/396 (79.29%), Query Frame = 0

Query: 1   MAMKMAIRASAVTPHHSLPSLPSSSQRSDSKSQFRPISVGLPASTTLSMLALISS-PYEA 60
           MAM      S+++   +LPS  SS++++ S    R +SV LP ST++S+L+L +S P+EA
Sbjct: 1   MAMAEMATKSSLSAKLTLPS--SSTKKTLS---LRQVSVSLPTSTSISLLSLFASPPHEA 60

Query: 61  RA---LNKDQIVSSLNDVEKTIDQVQEAGSNFFDIAQQLIDSARTILKPGVDAALPIVKQ 120
           +A   + KDQIVSSL +VEKTI+QVQE GS+ FD  Q++       LKP +D ALPI KQ
Sbjct: 61  KAAVSIPKDQIVSSLTEVEKTINQVQETGSSVFDATQRVFQVVGDALKPALDTALPIAKQ 120

Query: 121 AGEEALKVASPTISEASKKALEALQGSGIDTQPVLTAAKTVVGAAEQTGKVIEGAKPIAS 180
           AGEEA+K+ASP  SEASKKA EA+Q SG D++PV  AAKTV   A+QT K IE AKPIAS
Sbjct: 121 AGEEAMKLASPAFSEASKKAQEAMQSSGFDSEPVFNAAKTVTDVAQQTSKAIEDAKPIAS 180

Query: 181 STVETISTADPLVIAEIAGALAVAYLLFPPIWSAISFSFRGYKGELSPAQTLELISSSNY 240
           ST++TIS+ADP VI   AGA  +AYLL PP++SAISF+FRGYKG+L+PAQTL+L+ + NY
Sbjct: 181 STMDTISSADPSVIVVAAGAAFLAYLLLPPVFSAISFNFRGYKGDLTPAQTLDLLCTKNY 240

Query: 241 VLIDIRSEKDKDKSGIPRLPSSAKNQLIAIPLEELPSKLRGIVRNVKKLEAELSAIKISY 300
           +++DIRSEKDK+K+GIPRLPS+AKN++I+IPLEELP+K++GIVRN K++EAE++A+KISY
Sbjct: 241 LMVDIRSEKDKEKAGIPRLPSNAKNRVISIPLEELPNKVKGIVRNSKRVEAEIAALKISY 300

Query: 301 LKKLNKGSNVVILGSYSDSGKAVAKALTRLGFKNSWIVTDGFLGSKGWLQSRLGTDTYKF 360
           LKK+NKGSN++IL SY+DS K VAK L  LG+KN +IVTDGF G +GWLQSRLGTD+Y F
Sbjct: 301 LKKINKGSNIIILDSYTDSAKIVAKTLKVLGYKNCYIVTDGFSGGRGWLQSRLGTDSYNF 360

Query: 361 SFAEILSPSRVIPAATKRFGTTSLTTSGQKLLPGAD 393
           SFA++LSPSR+IPAA++ FG    T SG K LP +D
Sbjct: 361 SFAQVLSPSRIIPAASRSFG----TRSGTKFLPSSD 387

BLAST of Carg04789 vs. TAIR 10
Match: AT3G59780.1 (Rhodanese/Cell cycle control phosphatase superfamily protein )

HSP 1 Score: 68.9 bits (167), Expect = 1.0e-11
Identity = 85/344 (24.71%), Postives = 157/344 (45.64%), Query Frame = 0

Query: 24  SSQRSDSKSQFRPISVGLPASTTLSM----------LALISSPYEARALNKDQIV-SSLN 83
           SS   D+K+     S G+  S + S+          L   SS   +   N  ++V S++N
Sbjct: 196 SSSLPDAKASVDDFSSGVKESFSSSLNQGENAVKNTLESFSSSVTSITKNASEVVDSAVN 255

Query: 84  DVEKTIDQVQE-AGSNFFDIAQQLIDSARTILKPGVDAALPIVKQAGEEALKVASPTISE 143
               T+DQ  + AG  F   +  L +++       +D     V   GE ++      +  
Sbjct: 256 RAFSTLDQTGDVAGDKFSSFSTGLKEASNRAAVIAIDLLRQSV-SLGERSVTNGVSFVVY 315

Query: 144 ASKKALEALQGSGIDTQPVLTAAKTV-VGAAEQTGKVIEGAKPIASSTVETISTADP--- 203
           +   A E L     D +  L +++ V +      G V++    +A   +E     DP   
Sbjct: 316 SYGSAKELLPP---DVKSALNSSEDVALKVLSPVGAVLQQVS-VAIGGLERNIGLDPDDP 375

Query: 204 -LVIAEIAGALAVAYLLFPPIWSAISFSFRGYKGELSPAQTLELISS-SNYVLIDIRSEK 263
            L +    G     ++L+  +W     ++ GY G+LSP  TL+L+ S    VLID+R E 
Sbjct: 376 ILHLFLFVGTTGTFWVLY-RVW-----TYGGYAGDLSPKSTLDLLKSRDKSVLIDVRPEA 435

Query: 264 DKDKSGIPRLPSSAKNQLIAIPLEELPSKLRGIVRNVKKLEAELSAIKISYLKKLNKGSN 323
            ++K GIP L  SA+ +  ++ L E+   ++ +++   +++  L+A+ I  LK +   S 
Sbjct: 436 LREKDGIPDLRRSARFRYSSVTLPEVDGDVKRLLKGGSEVDDILTAVIIKNLKIVQDRSK 495

Query: 324 VVILGSYSDSGKAVAKALTRLGFKNSWIVTDGFLGSKGWLQSRL 350
           VV++ +     K +A+AL ++G K  +++  G+   + W+Q  L
Sbjct: 496 VVVMDADGTRSKGIARALRKVGIKRPYLMQGGY---RSWVQEGL 525

BLAST of Carg04789 vs. TAIR 10
Match: AT4G01050.1 (thylakoid rhodanese-like )

HSP 1 Score: 44.7 bits (104), Expect = 2.0e-04
Identity = 43/172 (25.00%), Postives = 76/172 (44.19%), Query Frame = 0

Query: 179 VETISTADPLVIAEIAGALAVAYLLFPPIWSAISFSFRGYKGELSPAQTLELISSSNYVL 238
           +    T +PLVIA    ALAV ++L       ++   + +  E +     +L +  N  L
Sbjct: 95  ISNFVTDNPLVIAGGVAALAVPFVLS----QVLNKKPKSWGVESAKNAYTKLGTDDNAQL 154

Query: 239 IDIRSEKDKDKSGIPRLPSSAKNQLIAI-PLEELPSKLRGIVRNVKKLEAELSAIKISYL 298
           +DIR+  D  + G P +    K  +  +   E+ P  L+                K+S  
Sbjct: 155 LDIRATADFRQVGSPNIKGLGKKAVSTVYNGEDKPGFLK----------------KLSLK 214

Query: 299 KKLNKGSNVVILGSYSDSGKAVAKALTRLGFKNSWIVTDGFLGSKGWLQSRL 350
            K  + + + IL  +  + + VA+ +   GFK+++ + DG  G +GWL S L
Sbjct: 215 FKDPENTTLYILDKFDGNSELVAELVALNGFKSAYAIKDGAEGPRGWLNSSL 246

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7019969.18.1e-205100.00Calcium sensing receptor, chloroplastic [Cucurbita argyrosperma subsp. argyrospe... [more]
KAG6584382.11.1e-20499.74Calcium sensing receptor, chloroplastic, partial [Cucurbita argyrosperma subsp. ... [more]
XP_022924156.18.9e-20499.49calcium sensing receptor, chloroplastic [Cucurbita moschata][more]
XP_023000782.12.9e-20298.47calcium sensing receptor, chloroplastic [Cucurbita maxima][more]
XP_023519333.13.7e-20298.21calcium sensing receptor, chloroplastic [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9FN485.6e-12461.11Calcium sensing receptor, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CAS P... [more]
B6TVL43.4e-8948.44Calcium sensing receptor, chloroplastic OS=Zea mays OX=4577 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1E8T44.3e-20499.49calcium sensing receptor, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1KEL81.4e-20298.47calcium sensing receptor, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111495... [more]
A0A5D3BLC01.8e-18188.83Calcium sensing receptor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A1S3C0H31.8e-18188.83calcium sensing receptor, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103495398 ... [more]
A0A0A0LSX71.1e-18088.58Rhodanese domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G041560 P... [more]
Match NameE-valueIdentityDescription
AT5G23060.13.9e-12561.11calcium sensing receptor [more]
AT3G59780.11.0e-1124.71Rhodanese/Cell cycle control phosphatase superfamily protein [more]
AT4G01050.12.0e-0425.00thylakoid rhodanese-like [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001763Rhodanese-like domainSMARTSM00450rhod_4coord: 222..350
e-value: 0.003
score: 26.8
IPR001763Rhodanese-like domainPROSITEPS50206RHODANESE_3coord: 232..353
score: 11.553666
IPR036873Rhodanese-like domain superfamilyGENE3D3.40.250.10coord: 219..358
e-value: 3.1E-15
score: 58.2
IPR036873Rhodanese-like domain superfamilySUPERFAMILY52821Rhodanese/Cell cycle control phosphatasecoord: 199..344
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 8..34
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 16..34
NoneNo IPR availablePANTHERPTHR34209:SF1CALCIUM SENSING RECEPTOR, CHLOROPLASTICcoord: 5..390
NoneNo IPR availableCDDcd00158RHODcoord: 226..340
e-value: 5.84913E-8
score: 48.0646
IPR044690Calcium sensing receptor, plantPANTHERPTHR34209RHODANESE/CELL CYCLE CONTROL PHOSPHATASE SUPERFAMILY PROTEINcoord: 5..390

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg04789-RACarg04789-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071277 cellular response to calcium ion
biological_process GO:0009704 de-etiolation
biological_process GO:0090333 regulation of stomatal closure
cellular_component GO:0016021 integral component of membrane