Carg04778 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg04778
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Usp domain-containing protein
Location	Carg_Chr13: 9327725 .. 9329402 (-)
RNA-Seq Expression	Carg04778
Synteny	Carg04778

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTGTTTCTGGGTTTGTTCTAGGCCACAACACAAAAAACCCATATCAGATTCTGTGGAGAATGTCCAAAAGAAGGAGCTTTTGAGGAGTGTTGTTAGTGGGGAAGAAGAGTTCAGCTCTGATAGCTCCAAAGCTTCGTCTGAAAATGGAAACAGAGTAATGGTGGTTGTGGATTGGAGCGTTGAGGCTGAAGGGGCGTTGGAATGGACGCTCTCCCATGCTGTTCGGAGTCGCGACACCATTGTTCTTGTTCATGTTCTCAAGAGTTTGAAGACTCAAGGTGAGAATTTCAGAGATTTGTGTTTCTGGGTTGAGATGGAACATGGCAGTGATTGTTTTTGTTTCTTATTTTTGCAGGTTTTGAGTTTGGGAATAAGGTGGATTCCACAAAGGAGGCCTATAAGCTTCTGTTTTCTATGAGAAACATGTGTTTAAAGAGAAGGCCTGAGGTAACTTCCTGTTAATACTATGCTTATAGACATATGCATACCAATACGTATCTAAATGTGTACAACTCAATTGCTTGAGTAGGTGCAAGTAGAGATGGCATTACTAGAAGGGAAAAAAAGAGGTCCAATAATTGTGGAAGAAGCAAAGAAGCATAAACTCTCTCTTTTGGTACTTGGTCAAAGAAAGAGGCCGATTCTTCGGCGTCTATTGAAAAGATGGGCGACAGGACGCTATCGAAGGAGAAGGAAGAAGAAGACTTGTCGAGCAACGACGGAGTATTGCATTCAGAACTCATCTTGCATGACCATTGCAGTGAGGAAGAAGAGCAAAAAGATTGGAGGATATTTGATCACGACCAAACGTCACAAGAACTTCTGGCTCTTGGCTTGATCTTTTTTCTTCTTTTCTTTTCTTTTTGTGTATCATATTCTTTCACCTTCTTAGGTTTATAAAAAGGTTCTTAAAAACTGTAGTTTCTGTTCTACAAAGAGTGATAAAAATATCCAGCTCTACAATTCTATTGTTCAAACAAATTGTTTAGTCAATCAGATTCTATAGCAGAGGTTGATGAAAGTTAGTCGGCCATCCTTCAAATGGAGGGACCGATACAGATTGTTAGGCTGGATTGATCGTCTTTCTTCGACAGACCGAGATTTAAAGGAAAAACAGGAAGAGAAACTGTAAGAACAGAACCAACAAATGTATTTTATTTTCAACTTGAAGTCATGAATTTCATAAACAACCAACAAATGTAGATGTATTTGAACTATACACAGGCAAAGGCATCGAATTCTGTGTAAATTCTAGGAGGGCATTTTCTAGTGAATAAACATCTTCAGCCTTTTAATTCCCTAGATCTACTTCAGGTTCAATTATGAAGTTGAACCAGAAAATAAAAGTTGAACATACCATTTTCGGTCATTTCGAGGCTATTTGATGTTGTTGCCCGCCATAGGAGTAACCATGTCGACATGCAATTTCGTGCAGTTGATCATAACCTGCAAGAACACCAGCCCCTGCAACACCAACAAGCATATTGGCAGTGACTCCTCGAAAAAGAGCAGCGATACCCTCGTGGCGAATGATCTCGGAAAATGCATGTAAAGCACTGCGGTACTTCAGGGTTTGTCCAGAGGTAAGCATCATTCTTCGCCGCAGCGTGTCGAAAGGATAAGCGCATACCCCAGAAAATGTTGTGATACTCCACCCCAATAAGAAACTAGCA

mRNA sequence

ATGGCTTGTTTCTGGGTTTGTTCTAGGCCACAACACAAAAAACCCATATCAGATTCTGTGGAGAATGTCCAAAAGAAGGAGCTTTTGAGGAGTGTTGTTAGTGGGGAAGAAGAGTTCAGCTCTGATAGCTCCAAAGCTTCGTCTGAAAATGGAAACAGAGTAATGGTGGTTGTGGATTGGAGCGTTGAGGCTGAAGGGGCGTTGGAATGGACGCTCTCCCATGCTGTTCGGAGTCGCGACACCATTGTTCTTGTTCATGTTCTCAAGAGTTTGAAGACTCAAGGTTTTGAGTTTGGGAATAAGGTGGATTCCACAAAGGAGGCCTATAAGCTTCTGTTTTCTATGAGAAACATGTGTTTAAAGAGAAGGCCTGAGGTGCAAGTAGAGATGGCATTACTAGAAGGGAAAAAAAGAGGTCCAATAATTGTGGAAGAAGCAAAGAAGCATAAACTCTCTCTTTTGGTACTTGGTCAAAGAAAGAGGCCGATTCTTCGGCGTCTATTGAAAAGATGGGCGACAGGACGCTATCGAAGGAGAAGGAAGAAGAAGACTTGTCGAGCAACGACGGAGTATTGCATTCAGAACTCATCTTGCATGACCATTGCAGTGAGGAAGAAGAGCAAAAAGATTGGAGGATATTTGATCACGACCAAACGTCACAAGAACTTCTGGCTCTTGGCTTGATCTTTTTTCTTCTTTTCTTTTCTTTTTGTGTATCATATTCTTTCACCTTCTTAGGTTTATAAAAAGGTTCTTAAAAACTGTAGTTTCTGTTCTACAAAGAGTGATAAAAATATCCAGCTCTACAATTCTATTGTTCAAACAAATTGTTTAGTCAATCAGATTCTATAGCAGAGGTTGATGAAAGTTAGTCGGCCATCCTTCAAATGGAGGGACCGATACAGATTGTTAGGCTGGATTGATCGTCTTTCTTCGACAGACCGAGATTTAAAGGAAAAACAGGAAGAGAAACTGTAAGAACAGAACCAACAAATGTATTTTATTTTCAACTTGAAGTCATGAATTTCATAAACAACCAACAAATGTAGATGTATTTGAACTATACACAGGCAAAGGCATCGAATTCTGTGTAAATTCTAGGAGGGCATTTTCTAGTGAATAAACATCTTCAGCCTTTTAATTCCCTAGATCTACTTCAGGTTCAATTATGAAGTTGAACCAGAAAATAAAAGTTGAACATACCATTTTCGGTCATTTCGAGGCTATTTGATGTTGTTGCCCGCCATAGGAGTAACCATGTCGACATGCAATTTCGTGCAGTTGATCATAACCTGCAAGAACACCAGCCCCTGCAACACCAACAAGCATATTGGCAGTGACTCCTCGAAAAAGAGCAGCGATACCCTCGTGGCGAATGATCTCGGAAAATGCATGTAAAGCACTGCGGTACTTCAGGGTTTGTCCAGAGGTAAGCATCATTCTTCGCCGCAGCGTGTCGAAAGGATAAGCGCATACCCCAGAAAATGTTGTGATACTCCACCCCAATAAGAAACTAGCA

Coding sequence (CDS)

ATGGCTTGTTTCTGGGTTTGTTCTAGGCCACAACACAAAAAACCCATATCAGATTCTGTGGAGAATGTCCAAAAGAAGGAGCTTTTGAGGAGTGTTGTTAGTGGGGAAGAAGAGTTCAGCTCTGATAGCTCCAAAGCTTCGTCTGAAAATGGAAACAGAGTAATGGTGGTTGTGGATTGGAGCGTTGAGGCTGAAGGGGCGTTGGAATGGACGCTCTCCCATGCTGTTCGGAGTCGCGACACCATTGTTCTTGTTCATGTTCTCAAGAGTTTGAAGACTCAAGGTTTTGAGTTTGGGAATAAGGTGGATTCCACAAAGGAGGCCTATAAGCTTCTGTTTTCTATGAGAAACATGTGTTTAAAGAGAAGGCCTGAGGTGCAAGTAGAGATGGCATTACTAGAAGGGAAAAAAAGAGGTCCAATAATTGTGGAAGAAGCAAAGAAGCATAAACTCTCTCTTTTGGTACTTGGTCAAAGAAAGAGGCCGATTCTTCGGCGTCTATTGAAAAGATGGGCGACAGGACGCTATCGAAGGAGAAGGAAGAAGAAGACTTGTCGAGCAACGACGGAGTATTGCATTCAGAACTCATCTTGCATGACCATTGCAGTGAGGAAGAAGAGCAAAAAGATTGGAGGATATTTGATCACGACCAAACGTCACAAGAACTTCTGGCTCTTGGCTTGA

Protein sequence

MACFWVCSRPQHKKPISDSVENVQKKELLRSVVSGEEEFSSDSSKASSENGNRVMVVVDWSVEAEGALEWTLSHAVRSRDTIVLVHVLKSLKTQGFEFGNKVDSTKEAYKLLFSMRNMCLKRRPEVQVEMALLEGKKRGPIIVEEAKKHKLSLLVLGQRKRPILRRLLKRWATGRYRRRRKKKTCRATTEYCIQNSSCMTIAVRKKSKKIGGYLITTKRHKNFWLLA

Homology

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BLAST of Carg04778 vs. NCBI nr
Match: KAG7019978.1 (hypothetical protein SDJN02_18946, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 445.3 bits (1144), Expect = 3.2e-121
Identity = 227/227 (100.00%), Postives = 227/227 (100.00%), Query Frame = 0


Query: 1   MACFWVCSRPQHKKPISDSVENVQKKELLRSVVSGEEEFSSDSSKASSENGNRVMVVVDW 60
           MACFWVCSRPQHKKPISDSVENVQKKELLRSVVSGEEEFSSDSSKASSENGNRVMVVVDW
Sbjct: 1   MACFWVCSRPQHKKPISDSVENVQKKELLRSVVSGEEEFSSDSSKASSENGNRVMVVVDW 60

Query: 61  SVEAEGALEWTLSHAVRSRDTIVLVHVLKSLKTQGFEFGNKVDSTKEAYKLLFSMRNMCL 120
           SVEAEGALEWTLSHAVRSRDTIVLVHVLKSLKTQGFEFGNKVDSTKEAYKLLFSMRNMCL
Sbjct: 61  SVEAEGALEWTLSHAVRSRDTIVLVHVLKSLKTQGFEFGNKVDSTKEAYKLLFSMRNMCL 120

Query: 121 KRRPEVQVEMALLEGKKRGPIIVEEAKKHKLSLLVLGQRKRPILRRLLKRWATGRYRRRR 180
           KRRPEVQVEMALLEGKKRGPIIVEEAKKHKLSLLVLGQRKRPILRRLLKRWATGRYRRRR
Sbjct: 121 KRRPEVQVEMALLEGKKRGPIIVEEAKKHKLSLLVLGQRKRPILRRLLKRWATGRYRRRR 180

Query: 181 KKKTCRATTEYCIQNSSCMTIAVRKKSKKIGGYLITTKRHKNFWLLA 228
           KKKTCRATTEYCIQNSSCMTIAVRKKSKKIGGYLITTKRHKNFWLLA
Sbjct: 181 KKKTCRATTEYCIQNSSCMTIAVRKKSKKIGGYLITTKRHKNFWLLA 227

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BLAST of Carg04778 vs. NCBI nr
Match: XP_022924122.1 (uncharacterized protein LOC111431650 [Cucurbita moschata])

HSP 1 Score: 434.1 bits (1115), Expect = 7.4e-118
Identity = 222/227 (97.80%), Postives = 224/227 (98.68%), Query Frame = 0


Query: 1   MACFWVCSRPQHKKPISDSVENVQKKELLRSVVSGEEEFSSDSSKASSENGNRVMVVVDW 60
           MACFWVCSRPQHKKPISDSVENVQKKELLRSVVSGEEEFSSDSSKASSENGNRVMVVVDW
Sbjct: 1   MACFWVCSRPQHKKPISDSVENVQKKELLRSVVSGEEEFSSDSSKASSENGNRVMVVVDW 60

Query: 61  SVEAEGALEWTLSHAVRSRDTIVLVHVLKSLKTQGFEFGNKVDSTKEAYKLLFSMRNMCL 120
           SVEAEGALEWTLSHAVRS DTIVLVHVLKSLKTQGFEFGNKV+STKEAYKLLFSMRNMCL
Sbjct: 61  SVEAEGALEWTLSHAVRSHDTIVLVHVLKSLKTQGFEFGNKVNSTKEAYKLLFSMRNMCL 120

Query: 121 KRRPEVQVEMALLEGKKRGPIIVEEAKKHKLSLLVLGQRKRPILRRLLKRWATGRYRRRR 180
           KRRPEVQVEMALLEGK+RGPIIVEEAKKHKLSLLVLGQRKR ILRRLLKRWATGRYRRRR
Sbjct: 121 KRRPEVQVEMALLEGKERGPIIVEEAKKHKLSLLVLGQRKRLILRRLLKRWATGRYRRRR 180

Query: 181 KKKTCRATTEYCIQNSSCMTIAVRKKSKKIGGYLITTKRHKNFWLLA 228
           KKKTCRAT EYCIQNSSCMTIAVRKKSKKIGGYLITTKRHKNFWLLA
Sbjct: 181 KKKTCRATAEYCIQNSSCMTIAVRKKSKKIGGYLITTKRHKNFWLLA 227

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BLAST of Carg04778 vs. NCBI nr
Match: XP_023001141.1 (uncharacterized protein LOC111495368 [Cucurbita maxima])

HSP 1 Score: 427.2 bits (1097), Expect = 9.0e-116
Identity = 218/227 (96.04%), Postives = 223/227 (98.24%), Query Frame = 0


Query: 1   MACFWVCSRPQHKKPISDSVENVQKKELLRSVVSGEEEFSSDSSKASSENGNRVMVVVDW 60
           MACFWVCSRPQHKKPISDSVENVQKKELL SVVSGEEEFSSDSSKASSENGNRVMVVVDW
Sbjct: 1   MACFWVCSRPQHKKPISDSVENVQKKELLGSVVSGEEEFSSDSSKASSENGNRVMVVVDW 60

Query: 61  SVEAEGALEWTLSHAVRSRDTIVLVHVLKSLKTQGFEFGNKVDSTKEAYKLLFSMRNMCL 120
           SVEAEGALEWTLSHAVRS DTIVLV+VLKSLK +GFEFGNKV+STKEAYKLLFSMRNMCL
Sbjct: 61  SVEAEGALEWTLSHAVRSHDTIVLVNVLKSLKPRGFEFGNKVNSTKEAYKLLFSMRNMCL 120

Query: 121 KRRPEVQVEMALLEGKKRGPIIVEEAKKHKLSLLVLGQRKRPILRRLLKRWATGRYRRRR 180
           KRRPEVQVEMALLEGK+RGPIIVEEAKKHKLSLLVLGQRKRPILRRLLKRWATGRYRRRR
Sbjct: 121 KRRPEVQVEMALLEGKERGPIIVEEAKKHKLSLLVLGQRKRPILRRLLKRWATGRYRRRR 180

Query: 181 KKKTCRATTEYCIQNSSCMTIAVRKKSKKIGGYLITTKRHKNFWLLA 228
           KKK+CRAT EYCIQNSSCMTIAVRKKSKKIGGYLITTKRHKNFWLLA
Sbjct: 181 KKKSCRATAEYCIQNSSCMTIAVRKKSKKIGGYLITTKRHKNFWLLA 227

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BLAST of Carg04778 vs. NCBI nr
Match: XP_023520317.1 (uncharacterized protein LOC111783631 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 422.9 bits (1086), Expect = 1.7e-114
Identity = 216/227 (95.15%), Postives = 219/227 (96.48%), Query Frame = 0


Query: 1   MACFWVCSRPQHKKPISDSVENVQKKELLRSVVSGEEEFSSDSSKASSENGNRVMVVVDW 60
           MACFWVCSRPQ KKPISDSVENVQKKELL SVVSGEEEFSSDSSK SS NGNRVMVVVDW
Sbjct: 1   MACFWVCSRPQRKKPISDSVENVQKKELLGSVVSGEEEFSSDSSKGSSANGNRVMVVVDW 60

Query: 61  SVEAEGALEWTLSHAVRSRDTIVLVHVLKSLKTQGFEFGNKVDSTKEAYKLLFSMRNMCL 120
           SVEAEGALEWTLSHAVRS DTIVLVHVLKSLK QGFEFGNKV+STKEAYKLLFSMRNMCL
Sbjct: 61  SVEAEGALEWTLSHAVRSHDTIVLVHVLKSLKPQGFEFGNKVNSTKEAYKLLFSMRNMCL 120

Query: 121 KRRPEVQVEMALLEGKKRGPIIVEEAKKHKLSLLVLGQRKRPILRRLLKRWATGRYRRRR 180
           KRRPEV VEMALLEGK+RGPIIVEEA+KHKLSLLVLGQRKRPILRRLLKRWATGRYRRRR
Sbjct: 121 KRRPEVHVEMALLEGKERGPIIVEEARKHKLSLLVLGQRKRPILRRLLKRWATGRYRRRR 180

Query: 181 KKKTCRATTEYCIQNSSCMTIAVRKKSKKIGGYLITTKRHKNFWLLA 228
           KKKTCRAT EYCIQNSSCMTIAVRKKSKKIGGYLITTKRHKNFWLLA
Sbjct: 181 KKKTCRATAEYCIQNSSCMTIAVRKKSKKIGGYLITTKRHKNFWLLA 227

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BLAST of Carg04778 vs. NCBI nr
Match: KAG6584393.1 (Agamous-like MADS-box protein AP1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 403.3 bits (1035), Expect = 1.4e-108
Identity = 209/210 (99.52%), Postives = 209/210 (99.52%), Query Frame = 0


Query: 18  DSVENVQKKELLRSVVSGEEEFSSDSSKASSENGNRVMVVVDWSVEAEGALEWTLSHAVR 77
           DSVENVQKKELLRSVVSGEEEFSSDSSKASSENGNRVMVVVDWSVEAEGALEWTLSHAVR
Sbjct: 247 DSVENVQKKELLRSVVSGEEEFSSDSSKASSENGNRVMVVVDWSVEAEGALEWTLSHAVR 306

Query: 78  SRDTIVLVHVLKSLKTQGFEFGNKVDSTKEAYKLLFSMRNMCLKRRPEVQVEMALLEGKK 137
           S DTIVLVHVLKSLKTQGFEFGNKVDSTKEAYKLLFSMRNMCLKRRPEVQVEMALLEGKK
Sbjct: 307 SHDTIVLVHVLKSLKTQGFEFGNKVDSTKEAYKLLFSMRNMCLKRRPEVQVEMALLEGKK 366

Query: 138 RGPIIVEEAKKHKLSLLVLGQRKRPILRRLLKRWATGRYRRRRKKKTCRATTEYCIQNSS 197
           RGPIIVEEAKKHKLSLLVLGQRKRPILRRLLKRWATGRYRRRRKKKTCRATTEYCIQNSS
Sbjct: 367 RGPIIVEEAKKHKLSLLVLGQRKRPILRRLLKRWATGRYRRRRKKKTCRATTEYCIQNSS 426

Query: 198 CMTIAVRKKSKKIGGYLITTKRHKNFWLLA 228
           CMTIAVRKKSKKIGGYLITTKRHKNFWLLA
Sbjct: 427 CMTIAVRKKSKKIGGYLITTKRHKNFWLLA 456

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BLAST of Carg04778 vs. ExPASy TrEMBL
Match: A0A6J1E832 (uncharacterized protein LOC111431650 OS=Cucurbita moschata OX=3662 GN=LOC111431650 PE=4 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 3.6e-118
Identity = 222/227 (97.80%), Postives = 224/227 (98.68%), Query Frame = 0


Query: 1   MACFWVCSRPQHKKPISDSVENVQKKELLRSVVSGEEEFSSDSSKASSENGNRVMVVVDW 60
           MACFWVCSRPQHKKPISDSVENVQKKELLRSVVSGEEEFSSDSSKASSENGNRVMVVVDW
Sbjct: 1   MACFWVCSRPQHKKPISDSVENVQKKELLRSVVSGEEEFSSDSSKASSENGNRVMVVVDW 60

Query: 61  SVEAEGALEWTLSHAVRSRDTIVLVHVLKSLKTQGFEFGNKVDSTKEAYKLLFSMRNMCL 120
           SVEAEGALEWTLSHAVRS DTIVLVHVLKSLKTQGFEFGNKV+STKEAYKLLFSMRNMCL
Sbjct: 61  SVEAEGALEWTLSHAVRSHDTIVLVHVLKSLKTQGFEFGNKVNSTKEAYKLLFSMRNMCL 120

Query: 121 KRRPEVQVEMALLEGKKRGPIIVEEAKKHKLSLLVLGQRKRPILRRLLKRWATGRYRRRR 180
           KRRPEVQVEMALLEGK+RGPIIVEEAKKHKLSLLVLGQRKR ILRRLLKRWATGRYRRRR
Sbjct: 121 KRRPEVQVEMALLEGKERGPIIVEEAKKHKLSLLVLGQRKRLILRRLLKRWATGRYRRRR 180

Query: 181 KKKTCRATTEYCIQNSSCMTIAVRKKSKKIGGYLITTKRHKNFWLLA 228
           KKKTCRAT EYCIQNSSCMTIAVRKKSKKIGGYLITTKRHKNFWLLA
Sbjct: 181 KKKTCRATAEYCIQNSSCMTIAVRKKSKKIGGYLITTKRHKNFWLLA 227

[Close]

BLAST of Carg04778 vs. ExPASy TrEMBL
Match: A0A6J1KHT2 (uncharacterized protein LOC111495368 OS=Cucurbita maxima OX=3661 GN=LOC111495368 PE=4 SV=1)

HSP 1 Score: 427.2 bits (1097), Expect = 4.4e-116
Identity = 218/227 (96.04%), Postives = 223/227 (98.24%), Query Frame = 0


Query: 1   MACFWVCSRPQHKKPISDSVENVQKKELLRSVVSGEEEFSSDSSKASSENGNRVMVVVDW 60
           MACFWVCSRPQHKKPISDSVENVQKKELL SVVSGEEEFSSDSSKASSENGNRVMVVVDW
Sbjct: 1   MACFWVCSRPQHKKPISDSVENVQKKELLGSVVSGEEEFSSDSSKASSENGNRVMVVVDW 60

Query: 61  SVEAEGALEWTLSHAVRSRDTIVLVHVLKSLKTQGFEFGNKVDSTKEAYKLLFSMRNMCL 120
           SVEAEGALEWTLSHAVRS DTIVLV+VLKSLK +GFEFGNKV+STKEAYKLLFSMRNMCL
Sbjct: 61  SVEAEGALEWTLSHAVRSHDTIVLVNVLKSLKPRGFEFGNKVNSTKEAYKLLFSMRNMCL 120

Query: 121 KRRPEVQVEMALLEGKKRGPIIVEEAKKHKLSLLVLGQRKRPILRRLLKRWATGRYRRRR 180
           KRRPEVQVEMALLEGK+RGPIIVEEAKKHKLSLLVLGQRKRPILRRLLKRWATGRYRRRR
Sbjct: 121 KRRPEVQVEMALLEGKERGPIIVEEAKKHKLSLLVLGQRKRPILRRLLKRWATGRYRRRR 180

Query: 181 KKKTCRATTEYCIQNSSCMTIAVRKKSKKIGGYLITTKRHKNFWLLA 228
           KKK+CRAT EYCIQNSSCMTIAVRKKSKKIGGYLITTKRHKNFWLLA
Sbjct: 181 KKKSCRATAEYCIQNSSCMTIAVRKKSKKIGGYLITTKRHKNFWLLA 227

[Close]

BLAST of Carg04778 vs. ExPASy TrEMBL
Match: A0A0A0LQN9 (Usp domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G039920 PE=4 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 1.5e-92
Identity = 187/233 (80.26%), Postives = 205/233 (87.98%), Query Frame = 0


Query: 1   MACFWVCSRPQHKKPISDSVENVQKKELLRSVVSGEEEFSSDSSK-ASSENGNRVMVVVD 60
           MACF +CS+ Q + PISDSVENVQK+ELL S VSGEE+FSSD+SK A  +NGNRVMVVVD
Sbjct: 1   MACFLLCSKSQQEIPISDSVENVQKRELLGSSVSGEEDFSSDNSKVALCQNGNRVMVVVD 60

Query: 61  WSVEAEGALEWTLSHAVRSRDTIVLVHVLKSLKTQ-----GFEFGNKVDSTKEAYKLLFS 120
           WSVEA+ ALEWTLSHAV+  DTIVLVHVLKSLK Q     GFEFGNKV+  K A+KLLFS
Sbjct: 61  WSVEAKEALEWTLSHAVQKNDTIVLVHVLKSLKLQRESFIGFEFGNKVNYIK-AHKLLFS 120

Query: 121 MRNMCLKRRPEVQVEMALLEGKKRGPIIVEEAKKHKLSLLVLGQRKRPILRRLLKRWATG 180
           MR+MCLK +PEVQVE+ALLEGK+RGPIIVEEAKKHKLSLLVLGQRKRP+LRRLL RWA  
Sbjct: 121 MRSMCLKTKPEVQVEVALLEGKERGPIIVEEAKKHKLSLLVLGQRKRPLLRRLLNRWAKR 180

Query: 181 RYRRRRKKKTCRATTEYCIQNSSCMTIAVRKKSKKIGGYLITTKRHKNFWLLA 228
           R RRR+KKKTCRAT EYCIQNSSCMTIAVRKKSK+IGGYLITTK HKNFWLLA
Sbjct: 181 RSRRRKKKKTCRATAEYCIQNSSCMTIAVRKKSKRIGGYLITTKSHKNFWLLA 232

[Close]

BLAST of Carg04778 vs. ExPASy TrEMBL
Match: A0A5D3BIQ2 (Putative Adenine nucleotide alpha hydrolases-like superfamily protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G001580 PE=4 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 4.9e-91
Identity = 185/233 (79.40%), Postives = 202/233 (86.70%), Query Frame = 0


Query: 1   MACFWVCSRPQHKKPISDSVENVQKKELLRSVVSGEEEFSSDSSKASSENGNRVMVVVDW 60
           MACF +CS+ Q + PISDSVENVQK+ELL S VSGE +FSSD+ KA  +NGNRVMVVVDW
Sbjct: 3   MACFLLCSKLQQENPISDSVENVQKRELLGSGVSGEGDFSSDNPKALCQNGNRVMVVVDW 62

Query: 61  SVEAEGALEWTLSHAVRSRDTIVLVHVLKSLKTQ-----GFEFGNKVDSTKEAYKLLFSM 120
           SVEA+ ALEWTLSHAV+  DTIVLVHVLKSLK Q     GFEFGNKV+  K A+KLLFSM
Sbjct: 63  SVEAKEALEWTLSHAVQKNDTIVLVHVLKSLKLQRESFIGFEFGNKVNYIK-AHKLLFSM 122

Query: 121 RNMCLKRRPEVQVEMALLEGKKRGPIIVEEAKKHKLSLLVLGQRKRPILRRLLKRWATGR 180
           R+MCLK +PEVQVE+ALLEGK+RGPIIVEEAKKHKLSLLVLGQRKRP+LRRL  RWA  R
Sbjct: 123 RSMCLKAKPEVQVEVALLEGKERGPIIVEEAKKHKLSLLVLGQRKRPLLRRLFNRWAKRR 182

Query: 181 YRRR-RKKKTCRATTEYCIQNSSCMTIAVRKKSKKIGGYLITTKRHKNFWLLA 228
            RRR +KKKTCRAT EYCIQNSSCMTIAVRKKSKKIGGYLITTK HKNFWLLA
Sbjct: 183 SRRRKKKKKTCRATAEYCIQNSSCMTIAVRKKSKKIGGYLITTKSHKNFWLLA 234

[Close]

BLAST of Carg04778 vs. ExPASy TrEMBL
Match: A0A6J1C707 (uncharacterized protein LOC111008987 OS=Momordica charantia OX=3673 GN=LOC111008987 PE=4 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 2.9e-83
Identity = 171/229 (74.67%), Postives = 192/229 (83.84%), Query Frame = 0


Query: 1   MACFWVCSRPQHKKPISDSVENVQKKELLRSVVSGEEEFSSDSSKASSENGNRVMVVVDW 60
           MA FWVCS P  K    +  +  +K ELLR   SGEEE SS+SSK+ SENGNRVMVVVDW
Sbjct: 1   MAGFWVCSGPNKK----NKKKKKKKNELLR---SGEEELSSNSSKSVSENGNRVMVVVDW 60

Query: 61  SVEAEGALEWTLSHAVRSRDTIVLVHVLKSLKTQGFEFGNKVDSTKEAYKLLFSMRNMCL 120
           S+EA+GAL+WTLSHA+ + DTI+L+HVLK+   QGF F NKVD  K AY+LLFSMRNMCL
Sbjct: 61  SIEAQGALQWTLSHALHTHDTILLLHVLKTSNKQGFGFNNKVDYIK-AYELLFSMRNMCL 120

Query: 121 KRRPEVQVEMALLEGKKRGPIIVEEAKKHKLSLLVLGQRKRPILRRLLKRWATGRYRRRR 180
           KRRPEVQVE+ALLEGK+RGP+IVEEAKKHKLSLLVLGQRKRPILRRLL RWAT   RR R
Sbjct: 121 KRRPEVQVEIALLEGKERGPVIVEEAKKHKLSLLVLGQRKRPILRRLLNRWATRSDRRGR 180

Query: 181 KKK--TCRATTEYCIQNSSCMTIAVRKKSKKIGGYLITTKRHKNFWLLA 228
           KKK  TCR T EYCIQNSSC+TIAVRKKS++IGGYLITTKRH+NFWLLA
Sbjct: 181 KKKKTTCRGTVEYCIQNSSCLTIAVRKKSRQIGGYLITTKRHRNFWLLA 221

[Close]

BLAST of Carg04778 vs. TAIR 10
Match: AT5G17390.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 160.6 bits (405), Expect = 1.5e-39
Identity = 93/205 (45.37%), Postives = 138/205 (67.32%), Query Frame = 0


Query: 25  KKELLRSVVSGEEEFSSDSSKASSENGNRVMVVVDWSVEAEGALEWTLSHAVRSRDTIVL 84
           KKE + +V + EEE         +E GNRVMVVVD ++ + GALEW ++H ++ +DT+ L
Sbjct: 99  KKEAVAAVEAVEEE---------AEGGNRVMVVVDKALASTGALEWAITHTLQPQDTLFL 158

Query: 85  VHVLKSLKTQGFEFGNKVDSTKEAYKLLFSMRNMCLKRRPEVQVEMALLEG--KKRGPII 144
           ++  K  +    +   +   T E   L+ +++ +C  +RP ++VE+  LEG  K +G  I
Sbjct: 159 LYFAKPFRKSKRKNRKREVKTDE---LVHTLKKLCQTKRPGIEVEIRRLEGKDKDKGQKI 218

Query: 145 VEEAKKHKLSLLVLGQRKRPILRRLLKRWATGRYRRRRKKKTCRATTEYCIQNSSCMTIA 204
           VEE+KK ++SLLV+GQ K+P + RLLKRWA   ++RRR  +      +YC++N+SCMTIA
Sbjct: 219 VEESKKQQVSLLVVGQEKKPPVWRLLKRWA---WKRRRGHE---GVLKYCLENASCMTIA 278

Query: 205 VRKKSKKIGGYLITTKRHKNFWLLA 228
           V+ K++K+GGYLITTKRHKNFWLLA
Sbjct: 279 VKPKNRKLGGYLITTKRHKNFWLLA 285

[Close]

BLAST of Carg04778 vs. TAIR 10
Match: AT1G69080.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 154.5 bits (389), Expect = 1.1e-37
Identity = 84/186 (45.16%), Postives = 121/186 (65.05%), Query Frame = 0


Query: 51  GNRVMVVVDWSVEAEGALEWTLSHAVRSRDTIVLVHVLKSLKTQGFEFGNKVD------- 110
           G R++VVVD   EA+ AL WTLSH  + +D+I+L+H LK+  +Q  +  NK +       
Sbjct: 43  GRRIIVVVDSCSEAKNALLWTLSHCAQPQDSILLLHFLKAKTSQSGDLANKEEGEDESCD 102

Query: 111 --STKEAYKLLFSMRNMCLKRRPEVQVEMALLEGKKRGPIIVEEAKKHKLSLLVLGQRKR 170
             +T  A K + +++ MC  +RPEV+ E+  ++G ++GP IV+EA++ + SLLVLGQ+K+
Sbjct: 103 KPTTSRADKKVSALKTMCELKRPEVKTEVVFVKGDEKGPTIVKEAREREASLLVLGQKKQ 162

Query: 171 PILRRLLKRWATGRYRRRRKKKTCRATTEYCIQNSSCMTIAVRKKSKKIGGYLITTKRHK 228
               RLL  WA+     + +  T     EYCI NS CM IAVRK+ KK+GGY +TTKRHK
Sbjct: 163 HATWRLLMVWAS-----QARPVTKHDFVEYCINNSPCMAIAVRKRGKKLGGYTLTTKRHK 222

[Close]

BLAST of Carg04778 vs. TAIR 10
Match: AT3G03290.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 150.2 bits (378), Expect = 2.0e-36
Identity = 84/182 (46.15%), Postives = 128/182 (70.33%), Query Frame = 0


Query: 48  SENGNRVMVVVDWSVEAEGALEWTLSHAVRSRDTIVLVHVLKSLKTQGFEFGNKVDSTKE 107
           +E GNRVMVVVD  + + GALEW L H ++S+D + L++  K  + +G     K  S  +
Sbjct: 102 TEAGNRVMVVVDKVIASTGALEWALKHTLQSQDYLFLLYFSKPFR-KGKRKNRK--SEVK 161

Query: 108 AYKLLFSMRNMCLKRRPEVQVEMALLEG--KKRGPIIVEEAKKHKLSLLVLGQRKRPILR 167
             +L+ +++ +C  +RP ++VE+  L+G  K++G  IVEEAK+ ++SLLV+G+ K+P + 
Sbjct: 162 TDELVHTLKKLCQTKRPGIEVEIRRLQGKEKEKGEKIVEEAKEQQVSLLVVGKEKKPPVW 221

Query: 168 RLLKRWATGRYRRRRKKKTCRATTEYCIQNSSCMTIAVRKKSKKIGGYLITTKRHKNFWL 227
           RLLKRW    +++RR +     T +YC++ +SCMTIAV+ K++K+GGYLITTKRHKNFWL
Sbjct: 222 RLLKRWG---WKKRRGR---AGTLKYCLEKASCMTIAVKPKNRKLGGYLITTKRHKNFWL 274

[Close]

BLAST of Carg04778 vs. TAIR 10
Match: AT1G69080.2 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 138.3 bits (347), Expect = 7.8e-33
Identity = 77/177 (43.50%), Postives = 112/177 (63.28%), Query Frame = 0


Query: 51  GNRVMVVVDWSVEAEGALEWTLSHAVRSRDTIVLVHVLKSLKTQGFEFGNKVDSTKEAYK 110
           G R++VVVD   EA+ AL WTLSH  + +D+I+L+H LK+  +Q  +  NK +   E+  
Sbjct: 43  GRRIIVVVDSCSEAKNALLWTLSHCAQPQDSILLLHFLKAKTSQSGDLANKEEGEDESCD 102

Query: 111 LLFSMRNMCLKRRPEVQVEMALLEGKKRGPIIVEEAKKHKLSLLVLGQRKRPILRRLLKR 170
                +    +   +V+ E+  ++G ++GP IV+EA++ + SLLVLGQ+K+    RLL  
Sbjct: 103 -----KPTTSRADKKVKTEVVFVKGDEKGPTIVKEAREREASLLVLGQKKQHATWRLLMV 162

Query: 171 WATGRYRRRRKKKTCRATTEYCIQNSSCMTIAVRKKSKKIGGYLITTKRHKNFWLLA 228
           WA+     + +  T     EYCI NS CM IAVRK+ KK+GGY +TTKRHK+FWLLA
Sbjct: 163 WAS-----QARPVTKHDFVEYCINNSPCMAIAVRKRGKKLGGYTLTTKRHKDFWLLA 209

[Close]

BLAST of Carg04778 vs. TAIR 10
Match: AT2G03720.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 130.6 bits (327), Expect = 1.6e-30
Identity = 75/174 (43.10%), Postives = 107/174 (61.49%), Query Frame = 0


Query: 55  MVVVDWSVEAEGALEWTLSHAVRSRDTIVLVHVLKSLKTQGFEFGNKVDSTKEAYKLLFS 114
           MVVVD + + + AL+W L+H V+  D I L+HV ++   Q  +   + +    A++L+  
Sbjct: 1   MVVVDTTSQTKNALQWALTHCVQDEDNITLLHVTRTPVGQAIDETQR-ERNSRAHELVHP 60

Query: 115 MRNMCLKRRPEVQVEMALLE-GKKRGPIIVEEAKKHKLSLLVLGQRKRPILRRLLKRWAT 174
           ++N C  ++P V+ E+ ++E  +++G  IVEE+KK    +LVLGQRKR    R++ +W  
Sbjct: 61  LKNFCQLKKPNVKTEIVVVETAEEKGKTIVEESKKQGAGVLVLGQRKRTSKWRVIWKW-- 120

Query: 175 GRYRRRRKKKTCRATTEYCIQNSSCMTIAVRKKSKKIGGYLITTKRHKNFWLLA 228
                R K        EYCI NS CM IAVRKKS   GGYLITTKRHK+FWLLA
Sbjct: 121 -----RTKGGMGGGVVEYCIHNSDCMAIAVRKKSNN-GGYLITTKRHKDFWLLA 165

The following BLAST results are available for this feature:

BLAST of Carg04778 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7019978.1	3.2e-121	100.00	hypothetical protein SDJN02_18946, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022924122.1	7.4e-118	97.80	uncharacterized protein LOC111431650 [Cucurbita moschata]	[more]
XP_023001141.1	9.0e-116	96.04	uncharacterized protein LOC111495368 [Cucurbita maxima]	[more]
XP_023520317.1	1.7e-114	95.15	uncharacterized protein LOC111783631 [Cucurbita pepo subsp. pepo]	[more]
KAG6584393.1	1.4e-108	99.52	Agamous-like MADS-box protein AP1, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of Carg04778 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg04778 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E832	3.6e-118	97.80	uncharacterized protein LOC111431650 OS=Cucurbita moschata OX=3662 GN=LOC1114316...	[more]
A0A6J1KHT2	4.4e-116	96.04	uncharacterized protein LOC111495368 OS=Cucurbita maxima OX=3661 GN=LOC111495368...	[more]
A0A0A0LQN9	1.5e-92	80.26	Usp domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G039920 PE=4 SV...	[more]
A0A5D3BIQ2	4.9e-91	79.40	Putative Adenine nucleotide alpha hydrolases-like superfamily protein OS=Cucumis...	[more]
A0A6J1C707	2.9e-83	74.67	uncharacterized protein LOC111008987 OS=Momordica charantia OX=3673 GN=LOC111008...	[more]

BLAST of Carg04778 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G17390.1	1.5e-39	45.37	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]
AT1G69080.1	1.1e-37	45.16	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]
AT3G03290.1	2.0e-36	46.15	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]
AT1G69080.2	7.8e-33	43.50	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]
AT2G03720.1	1.6e-30	43.10	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006016	UspA	PFAM	PF00582	Usp	coord: 53..204 e-value: 1.7E-19 score: 70.8
IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	GENE3D	3.40.50.620	HUPs	coord: 51..212 e-value: 2.2E-18 score: 68.8
None	No IPR available	PANTHER	PTHR47000	ADENINE NUCLEOTIDE ALPHA HYDROLASES-LIKE SUPERFAMILY PROTEIN	coord: 10..227
None	No IPR available	CDD	cd00293	USP_Like	coord: 53..203 e-value: 8.25685E-18 score: 74.7138
None	No IPR available	SUPERFAMILY	52402	Adenine nucleotide alpha hydrolases-like	coord: 52..205

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg04778-RA	Carg04778-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016787	hydrolase activity