Carg04737 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg04737
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description17.8 kDa class I heat shock protein-like
LocationCarg_Chr13: 9578125 .. 9579312 (+)
RNA-Seq ExpressionCarg04737
SyntenyCarg04737
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATATTTCTTCTCCATCTCTGCAGATTTCTTGTATATAAGAACAGAGGGGTCACCATATGGACTGCATTCAATCAAATTAGCCGCGAAGTAAACCAACAATGGAGAAAGGTGAAGCTTCGAATCTGTCGTACGAGGAATTCGAGCCTTTCTGCAAATGGAAAAAACTCGACGACTCCGACGTTCATGTTCTTGAGGTTCATCTCCCAGGTATTCATCAAATCAACTTACGGTCTCTTCTGTATGCTCATTATATTCTTAGTGATTTGAAATTGAAAACTGCACTTGTGAAACAGAATTCAAGAAAGAAGAATTGAGGGTTCGGATAAAGAACAACTCTATCCTTGCAATTTCCGGCGAGCACCTCGCCAATGATGGGAAGAAGATGCAATTCAACAGAGAAATCAAACTCCCAAAGGATATATACCCAGACCAAATCCGTGCCAAATTCGGTGGCTCTGTCCTTTCTATCACGATGCCTAAGAAACCCAGCTCGGCGGAAAGCAGCAAACCAAAGGCGGACGCCGCCTCGGAGGGTAATGACACAGCACCCAATGATGATGGTAAGCCAAAGAACTTGATATCCAGGTTAACAAGTCCATTATCGAGGCTGAAATTTAGCAAGAAAAAGGCGACGGCTTTGGCGGCGGCAGTGGTGATTCTGGCCTTCGGAGCTTACTACTTGATTAAAAACAGGGATTAAATTATATTCTTTGTTCGAACTGCATACATAGAATCAAATTGGATTCACCAATAATAAATCATGATTAATCTACCCTGTGAAGCTGAGAGTTCAATGGTGGGGGGCTTCCCAGATTATCCCCTTGGATTTCATTGATGATAGTTGTGTTCGTGTCTATAATACTTGCATTATACATTGATTGAAGCATTTTGATATGTCATTTTAGGTGTGCGTCGGTATCTTCAATTTTAATTTGTTCCCCAATTCTAGGCTTTTAGTGACACGTTACTCTGCATCAATGTATGTCTTAATTAAGTAGTCCCACAATCTCTGTCTCTTCTGCCAATGACCAATCTCAATCAAGATCAAGGAAGAATATCTACTTTTCTTGAATCACCTGAATCAAATAATGCTGCACTTCCAAAAGGAACTACTCAAATTTGAGTCCGGGATATCAATTTGGTACCATATAATTAGAAGTTCGAATCTCTAGATGAAATAATTAGAG

mRNA sequence

AATATTTCTTCTCCATCTCTGCAGATTTCTTGTATATAAGAACAGAGGGGTCACCATATGGACTGCATTCAATCAAATTAGCCGCGAAGTAAACCAACAATGGAGAAAGGTGAAGCTTCGAATCTGTCGTACGAGGAATTCGAGCCTTTCTGCAAATGGAAAAAACTCGACGACTCCGACGTTCATGTTCTTGAGGTTCATCTCCCAGAATTCAAGAAAGAAGAATTGAGGGTTCGGATAAAGAACAACTCTATCCTTGCAATTTCCGGCGAGCACCTCGCCAATGATGGGAAGAAGATGCAATTCAACAGAGAAATCAAACTCCCAAAGGATATATACCCAGACCAAATCCGTGCCAAATTCGGTGGCTCTGTCCTTTCTATCACGATGCCTAAGAAACCCAGCTCGGCGGAAAGCAGCAAACCAAAGGCGGACGCCGCCTCGGAGGGTAATGACACAGCACCCAATGATGATGGTAAGCCAAAGAACTTGATATCCAGGTTAACAAGTCCATTATCGAGGCTGAAATTTAGCAAGAAAAAGGCGACGGCTTTGGCGGCGGCAGTGGTGATTCTGGCCTTCGGAGCTTACTACTTGATTAAAAACAGGGATTAAATTATATTCTTTGTTCGAACTGCATACATAGAATCAAATTGGATTCACCAATAATAAATCATGATTAATCTACCCTGTGAAGCTGAGAGTTCAATGGTGGGGGGCTTCCCAGATTATCCCCTTGGATTTCATTGATGATAGTTGTGTTCGTGTCTATAATACTTGCATTATACATTGATTGAAGCATTTTGATATGTCATTTTAGGTGTGCGTCGGTATCTTCAATTTTAATTTGTTCCCCAATTCTAGGCTTTTAGTGACACGTTACTCTGCATCAATGTATGTCTTAATTAAGTAGTCCCACAATCTCTGTCTCTTCTGCCAATGACCAATCTCAATCAAGATCAAGGAAGAATATCTACTTTTCTTGAATCACCTGAATCAAATAATGCTGCACTTCCAAAAGGAACTACTCAAATTTGAGTCCGGGATATCAATTTGGTACCATATAATTAGAAGTTCGAATCTCTAGATGAAATAATTAGAG

Coding sequence (CDS)

ATGGAGAAAGGTGAAGCTTCGAATCTGTCGTACGAGGAATTCGAGCCTTTCTGCAAATGGAAAAAACTCGACGACTCCGACGTTCATGTTCTTGAGGTTCATCTCCCAGAATTCAAGAAAGAAGAATTGAGGGTTCGGATAAAGAACAACTCTATCCTTGCAATTTCCGGCGAGCACCTCGCCAATGATGGGAAGAAGATGCAATTCAACAGAGAAATCAAACTCCCAAAGGATATATACCCAGACCAAATCCGTGCCAAATTCGGTGGCTCTGTCCTTTCTATCACGATGCCTAAGAAACCCAGCTCGGCGGAAAGCAGCAAACCAAAGGCGGACGCCGCCTCGGAGGGTAATGACACAGCACCCAATGATGATGGTAAGCCAAAGAACTTGATATCCAGGTTAACAAGTCCATTATCGAGGCTGAAATTTAGCAAGAAAAAGGCGACGGCTTTGGCGGCGGCAGTGGTGATTCTGGCCTTCGGAGCTTACTACTTGATTAAAAACAGGGATTAA

Protein sequence

MEKGEASNLSYEEFEPFCKWKKLDDSDVHVLEVHLPEFKKEELRVRIKNNSILAISGEHLANDGKKMQFNREIKLPKDIYPDQIRAKFGGSVLSITMPKKPSSAESSKPKADAASEGNDTAPNDDGKPKNLISRLTSPLSRLKFSKKKATALAAAVVILAFGAYYLIKNRD
Homology
BLAST of Carg04737 vs. NCBI nr
Match: KAG7020019.1 (17.8 kDa class I heat shock protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 333.6 bits (854), Expect = 1.0e-87
Identity = 171/171 (100.00%), Postives = 171/171 (100.00%), Query Frame = 0

Query: 1   MEKGEASNLSYEEFEPFCKWKKLDDSDVHVLEVHLPEFKKEELRVRIKNNSILAISGEHL 60
           MEKGEASNLSYEEFEPFCKWKKLDDSDVHVLEVHLPEFKKEELRVRIKNNSILAISGEHL
Sbjct: 1   MEKGEASNLSYEEFEPFCKWKKLDDSDVHVLEVHLPEFKKEELRVRIKNNSILAISGEHL 60

Query: 61  ANDGKKMQFNREIKLPKDIYPDQIRAKFGGSVLSITMPKKPSSAESSKPKADAASEGNDT 120
           ANDGKKMQFNREIKLPKDIYPDQIRAKFGGSVLSITMPKKPSSAESSKPKADAASEGNDT
Sbjct: 61  ANDGKKMQFNREIKLPKDIYPDQIRAKFGGSVLSITMPKKPSSAESSKPKADAASEGNDT 120

Query: 121 APNDDGKPKNLISRLTSPLSRLKFSKKKATALAAAVVILAFGAYYLIKNRD 172
           APNDDGKPKNLISRLTSPLSRLKFSKKKATALAAAVVILAFGAYYLIKNRD
Sbjct: 121 APNDDGKPKNLISRLTSPLSRLKFSKKKATALAAAVVILAFGAYYLIKNRD 171

BLAST of Carg04737 vs. NCBI nr
Match: XP_022923784.1 (17.8 kDa class I heat shock protein-like [Cucurbita moschata] >KAG6584432.1 17.8 kDa class I heat shock protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 332.4 bits (851), Expect = 2.3e-87
Identity = 170/171 (99.42%), Postives = 171/171 (100.00%), Query Frame = 0

Query: 1   MEKGEASNLSYEEFEPFCKWKKLDDSDVHVLEVHLPEFKKEELRVRIKNNSILAISGEHL 60
           MEKGEASN+SYEEFEPFCKWKKLDDSDVHVLEVHLPEFKKEELRVRIKNNSILAISGEHL
Sbjct: 1   MEKGEASNVSYEEFEPFCKWKKLDDSDVHVLEVHLPEFKKEELRVRIKNNSILAISGEHL 60

Query: 61  ANDGKKMQFNREIKLPKDIYPDQIRAKFGGSVLSITMPKKPSSAESSKPKADAASEGNDT 120
           ANDGKKMQFNREIKLPKDIYPDQIRAKFGGSVLSITMPKKPSSAESSKPKADAASEGNDT
Sbjct: 61  ANDGKKMQFNREIKLPKDIYPDQIRAKFGGSVLSITMPKKPSSAESSKPKADAASEGNDT 120

Query: 121 APNDDGKPKNLISRLTSPLSRLKFSKKKATALAAAVVILAFGAYYLIKNRD 172
           APNDDGKPKNLISRLTSPLSRLKFSKKKATALAAAVVILAFGAYYLIKNRD
Sbjct: 121 APNDDGKPKNLISRLTSPLSRLKFSKKKATALAAAVVILAFGAYYLIKNRD 171

BLAST of Carg04737 vs. NCBI nr
Match: XP_023520341.1 (17.8 kDa class I heat shock protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 328.6 bits (841), Expect = 3.3e-86
Identity = 168/171 (98.25%), Postives = 169/171 (98.83%), Query Frame = 0

Query: 1   MEKGEASNLSYEEFEPFCKWKKLDDSDVHVLEVHLPEFKKEELRVRIKNNSILAISGEHL 60
           MEKGEASN+SYEEFEPFCKWKKLDDSDVHVLEVHLPEFKKEELRVRIKNNSILAISGEHL
Sbjct: 1   MEKGEASNVSYEEFEPFCKWKKLDDSDVHVLEVHLPEFKKEELRVRIKNNSILAISGEHL 60

Query: 61  ANDGKKMQFNREIKLPKDIYPDQIRAKFGGSVLSITMPKKPSSAESSKPKADAASEGNDT 120
           ANDGKKMQFNREIKLPKDIYPDQIRAKFGGSVLSITMPKKPSSAE  KPKADAASEGNDT
Sbjct: 61  ANDGKKMQFNREIKLPKDIYPDQIRAKFGGSVLSITMPKKPSSAEIGKPKADAASEGNDT 120

Query: 121 APNDDGKPKNLISRLTSPLSRLKFSKKKATALAAAVVILAFGAYYLIKNRD 172
           APNDDGKPKNLISRLTSPLSRLKFSKKKATALAAAVVILAFGAYYLIKNRD
Sbjct: 121 APNDDGKPKNLISRLTSPLSRLKFSKKKATALAAAVVILAFGAYYLIKNRD 171

BLAST of Carg04737 vs. NCBI nr
Match: XP_023001660.1 (17.8 kDa class I heat shock protein-like [Cucurbita maxima])

HSP 1 Score: 320.9 bits (821), Expect = 6.9e-84
Identity = 165/172 (95.93%), Postives = 169/172 (98.26%), Query Frame = 0

Query: 1   MEKGEASNLSYEEFEPFCKWKKLDDSDVHVLEVHLPEFKKEELRVRIKNNSILAISGEHL 60
           MEKGEASN+SYEEFEPFCKWKKLDDSDVHVLEVHLPEFKKEELRVRIKNNSILAISGEHL
Sbjct: 1   MEKGEASNVSYEEFEPFCKWKKLDDSDVHVLEVHLPEFKKEELRVRIKNNSILAISGEHL 60

Query: 61  -ANDGKKMQFNREIKLPKDIYPDQIRAKFGGSVLSITMPKKPSSAESSKPKADAASEGND 120
            ANDGKKMQFNREIKLPKDIYPDQIRAKFGGSVLSITMPKKPSSAE  KPKADAASEGND
Sbjct: 61  TANDGKKMQFNREIKLPKDIYPDQIRAKFGGSVLSITMPKKPSSAEIGKPKADAASEGND 120

Query: 121 TAPNDDGKPKNLISRLTSPLSRLKFSKKKATALAAAVVILAFGAYYLIKNRD 172
           TAPNDDG+PKNLISRLTSPLSRLKFSKKKATALAAAV+ILAFGAYYL+KNRD
Sbjct: 121 TAPNDDGEPKNLISRLTSPLSRLKFSKKKATALAAAVLILAFGAYYLVKNRD 172

BLAST of Carg04737 vs. NCBI nr
Match: XP_008465577.1 (PREDICTED: 16.9 kDa class I heat shock protein 1 [Cucumis melo] >KAA0036046.1 16.9 kDa class I heat shock protein 1 [Cucumis melo var. makuwa] >TYJ98853.1 16.9 kDa class I heat shock protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 248.1 bits (632), Expect = 5.7e-62
Identity = 134/170 (78.82%), Postives = 142/170 (83.53%), Query Frame = 0

Query: 1   MEKGEASNLSYEEFEPFCKWKKLDDSDVHVLEVHLPEFKKEELRVRIKNNSILAISGEHL 60
           MEKGE S + YEEFEPFCKWKKL+DSD  VLEVHLPEFKKEELRVRIKNNSIL +SGEHL
Sbjct: 1   MEKGEPSGVLYEEFEPFCKWKKLEDSD--VLEVHLPEFKKEELRVRIKNNSILTLSGEHL 60

Query: 61  -ANDGKKMQFNREIKLPKDIYPDQIRAKFGGSVLSITMPKKPSSAESSKPKADAASEGND 120
            A DGKKM FNREIKLPKDIYPD+IRAKFGG+VLSITMPKK S  E SKP  D ASE   
Sbjct: 61  TAKDGKKMHFNREIKLPKDIYPDEIRAKFGGNVLSITMPKKASPPEISKPNPDNASEDKL 120

Query: 121 TAPNDDGKPKNLISRLTSPLSRLKFSKKKATALAAAVVILAFGAYYLIKN 170
           T  N +GKPKN ISRL SPLSRLKFSK+ ATA+A AVVILA G YYLIKN
Sbjct: 121 TQSNGNGKPKNFISRLKSPLSRLKFSKETATAMAVAVVILAVGVYYLIKN 168

BLAST of Carg04737 vs. ExPASy Swiss-Prot
Match: Q9LNW0 (17.8 kDa class I heat shock protein OS=Arabidopsis thaliana OX=3702 GN=HSP17.8 PE=1 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 1.5e-09
Identity = 39/107 (36.45%), Postives = 63/107 (58.88%), Query Frame = 0

Query: 20  WKKLDDSDVHVLEVHLPEFKKEELRVRIKNNSILAISGE-HLANDGKK----------MQ 79
           WK  + ++ HV +  LP  KKEE++V I+++S+L ISGE H+  + K+           Q
Sbjct: 52  WK--ETAEAHVFKADLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGQ 111

Query: 80  FNREIKLPKDIYPDQIRAKFGGSVLSITMPKKPSSAESSKPKADAAS 116
           F+R+ KLP+++  DQ++A     VL++T+PK     E +K KA   S
Sbjct: 112 FSRKFKLPENVKMDQVKASMENGVLTVTVPK----VEEAKKKAQVKS 152

BLAST of Carg04737 vs. ExPASy Swiss-Prot
Match: Q9XIE3 (17.6 kDa class I heat shock protein 1 OS=Arabidopsis thaliana OX=3702 GN=HSP17.6A PE=1 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 9.9e-09
Identity = 36/107 (33.64%), Postives = 60/107 (56.07%), Query Frame = 0

Query: 20  WKKLDDSDVHVLEVHLPEFKKEELRVRIKNNSILAISGEHLANDGKKMQ----------- 79
           WK  + ++ HV +  LP  KKEE++V I+++S+L ISGE      +K             
Sbjct: 50  WK--ETAEAHVFKADLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGG 109

Query: 80  FNREIKLPKDIYPDQIRAKFGGSVLSITMPKKPSSAESSKPKADAAS 116
           F+R+ +LP+++  DQ++A     VL++T+PK     E++K KA   S
Sbjct: 110 FSRKFRLPENVKMDQVKASMENGVLTVTVPK----VETNKKKAQVKS 150

BLAST of Carg04737 vs. ExPASy Swiss-Prot
Match: Q9ZW31 (17.6 kDa class I heat shock protein 2 OS=Arabidopsis thaliana OX=3702 GN=HSP17.6B PE=2 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 4.9e-08
Identity = 31/84 (36.90%), Postives = 49/84 (58.33%), Query Frame = 0

Query: 27  DVHVLEVHLPEFKKEELRVRIKNNSILAISGE-HLANDGK----------KMQFNREIKL 86
           + HV +  LP  KKEE++V I+ +S+L ISGE H+  + K            QF R  +L
Sbjct: 55  EAHVFKADLPGLKKEEVKVEIEEDSVLKISGERHVEKEDKNDTWHRVERSSGQFTRRFRL 114

Query: 87  PKDIYPDQIRAKFGGSVLSITMPK 100
           P+++  DQ++A     VL++T+PK
Sbjct: 115 PENVKMDQVKAAMENGVLTVTVPK 138

BLAST of Carg04737 vs. ExPASy Swiss-Prot
Match: P31673 (17.4 kDa class I heat shock protein OS=Oryza sativa subsp. japonica OX=39947 GN=HSP17.4 PE=2 SV=2)

HSP 1 Score: 57.0 bits (136), Expect = 2.4e-07
Identity = 32/101 (31.68%), Postives = 55/101 (54.46%), Query Frame = 0

Query: 20  WKKLDDSDVHVLEVHLPEFKKEELRVRIKNNSILAISGEHLANDGKKM-----------Q 79
           WK  +  + HV +  +P  KKEE++V +++ ++L ISGE +    +K            +
Sbjct: 51  WK--ETPEAHVFKADVPGLKKEEVKVEVEDGNVLQISGERIKEQEEKTDKWHRVERSSGK 110

Query: 80  FNREIKLPKDIYPDQIRAKFGGSVLSITMPKKPSSAESSKP 110
           F R  +LP++  P+QI+A     VL++T+PK+    E  KP
Sbjct: 111 FLRRFRLPENTKPEQIKASMENGVLTVTVPKE----EPKKP 145

BLAST of Carg04737 vs. ExPASy Swiss-Prot
Match: Q84J50 (17.7 kDa class I heat shock protein OS=Oryza sativa subsp. japonica OX=39947 GN=HSP17.7 PE=2 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 3.2e-07
Identity = 34/101 (33.66%), Postives = 54/101 (53.47%), Query Frame = 0

Query: 20  WKKLDDSDVHVLEVHLPEFKKEELRVRIKNNSILAISGEHLANDGKKM-----------Q 79
           WK  +  +VHV +  +P  KKEE++V + + +IL ISGE      +K            +
Sbjct: 56  WK--ETPEVHVFKADVPGLKKEEVKVEVDDGNILQISGERSREQEEKSDKWHRVERSSGK 115

Query: 80  FNREIKLPKDIYPDQIRAKFGGSVLSITMPKKPSSAESSKP 110
           F R  +LP++  P+QI+A     VL++T+PK+    E  KP
Sbjct: 116 FLRRFRLPENTKPEQIKASMENGVLTVTVPKE----EPKKP 150

BLAST of Carg04737 vs. ExPASy TrEMBL
Match: A0A6J1E7D1 (17.8 kDa class I heat shock protein-like OS=Cucurbita moschata OX=3662 GN=LOC111431393 PE=3 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 1.1e-87
Identity = 170/171 (99.42%), Postives = 171/171 (100.00%), Query Frame = 0

Query: 1   MEKGEASNLSYEEFEPFCKWKKLDDSDVHVLEVHLPEFKKEELRVRIKNNSILAISGEHL 60
           MEKGEASN+SYEEFEPFCKWKKLDDSDVHVLEVHLPEFKKEELRVRIKNNSILAISGEHL
Sbjct: 1   MEKGEASNVSYEEFEPFCKWKKLDDSDVHVLEVHLPEFKKEELRVRIKNNSILAISGEHL 60

Query: 61  ANDGKKMQFNREIKLPKDIYPDQIRAKFGGSVLSITMPKKPSSAESSKPKADAASEGNDT 120
           ANDGKKMQFNREIKLPKDIYPDQIRAKFGGSVLSITMPKKPSSAESSKPKADAASEGNDT
Sbjct: 61  ANDGKKMQFNREIKLPKDIYPDQIRAKFGGSVLSITMPKKPSSAESSKPKADAASEGNDT 120

Query: 121 APNDDGKPKNLISRLTSPLSRLKFSKKKATALAAAVVILAFGAYYLIKNRD 172
           APNDDGKPKNLISRLTSPLSRLKFSKKKATALAAAVVILAFGAYYLIKNRD
Sbjct: 121 APNDDGKPKNLISRLTSPLSRLKFSKKKATALAAAVVILAFGAYYLIKNRD 171

BLAST of Carg04737 vs. ExPASy TrEMBL
Match: A0A6J1KLT1 (17.8 kDa class I heat shock protein-like OS=Cucurbita maxima OX=3661 GN=LOC111495735 PE=3 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 3.3e-84
Identity = 165/172 (95.93%), Postives = 169/172 (98.26%), Query Frame = 0

Query: 1   MEKGEASNLSYEEFEPFCKWKKLDDSDVHVLEVHLPEFKKEELRVRIKNNSILAISGEHL 60
           MEKGEASN+SYEEFEPFCKWKKLDDSDVHVLEVHLPEFKKEELRVRIKNNSILAISGEHL
Sbjct: 1   MEKGEASNVSYEEFEPFCKWKKLDDSDVHVLEVHLPEFKKEELRVRIKNNSILAISGEHL 60

Query: 61  -ANDGKKMQFNREIKLPKDIYPDQIRAKFGGSVLSITMPKKPSSAESSKPKADAASEGND 120
            ANDGKKMQFNREIKLPKDIYPDQIRAKFGGSVLSITMPKKPSSAE  KPKADAASEGND
Sbjct: 61  TANDGKKMQFNREIKLPKDIYPDQIRAKFGGSVLSITMPKKPSSAEIGKPKADAASEGND 120

Query: 121 TAPNDDGKPKNLISRLTSPLSRLKFSKKKATALAAAVVILAFGAYYLIKNRD 172
           TAPNDDG+PKNLISRLTSPLSRLKFSKKKATALAAAV+ILAFGAYYL+KNRD
Sbjct: 121 TAPNDDGEPKNLISRLTSPLSRLKFSKKKATALAAAVLILAFGAYYLVKNRD 172

BLAST of Carg04737 vs. ExPASy TrEMBL
Match: A0A5D3BHU7 (16.9 kDa class I heat shock protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00480 PE=3 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 2.7e-62
Identity = 134/170 (78.82%), Postives = 142/170 (83.53%), Query Frame = 0

Query: 1   MEKGEASNLSYEEFEPFCKWKKLDDSDVHVLEVHLPEFKKEELRVRIKNNSILAISGEHL 60
           MEKGE S + YEEFEPFCKWKKL+DSD  VLEVHLPEFKKEELRVRIKNNSIL +SGEHL
Sbjct: 1   MEKGEPSGVLYEEFEPFCKWKKLEDSD--VLEVHLPEFKKEELRVRIKNNSILTLSGEHL 60

Query: 61  -ANDGKKMQFNREIKLPKDIYPDQIRAKFGGSVLSITMPKKPSSAESSKPKADAASEGND 120
            A DGKKM FNREIKLPKDIYPD+IRAKFGG+VLSITMPKK S  E SKP  D ASE   
Sbjct: 61  TAKDGKKMHFNREIKLPKDIYPDEIRAKFGGNVLSITMPKKASPPEISKPNPDNASEDKL 120

Query: 121 TAPNDDGKPKNLISRLTSPLSRLKFSKKKATALAAAVVILAFGAYYLIKN 170
           T  N +GKPKN ISRL SPLSRLKFSK+ ATA+A AVVILA G YYLIKN
Sbjct: 121 TQSNGNGKPKNFISRLKSPLSRLKFSKETATAMAVAVVILAVGVYYLIKN 168

BLAST of Carg04737 vs. ExPASy TrEMBL
Match: A0A1S3CP71 (16.9 kDa class I heat shock protein 1 OS=Cucumis melo OX=3656 GN=LOC103502934 PE=3 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 2.7e-62
Identity = 134/170 (78.82%), Postives = 142/170 (83.53%), Query Frame = 0

Query: 1   MEKGEASNLSYEEFEPFCKWKKLDDSDVHVLEVHLPEFKKEELRVRIKNNSILAISGEHL 60
           MEKGE S + YEEFEPFCKWKKL+DSD  VLEVHLPEFKKEELRVRIKNNSIL +SGEHL
Sbjct: 1   MEKGEPSGVLYEEFEPFCKWKKLEDSD--VLEVHLPEFKKEELRVRIKNNSILTLSGEHL 60

Query: 61  -ANDGKKMQFNREIKLPKDIYPDQIRAKFGGSVLSITMPKKPSSAESSKPKADAASEGND 120
            A DGKKM FNREIKLPKDIYPD+IRAKFGG+VLSITMPKK S  E SKP  D ASE   
Sbjct: 61  TAKDGKKMHFNREIKLPKDIYPDEIRAKFGGNVLSITMPKKASPPEISKPNPDNASEDKL 120

Query: 121 TAPNDDGKPKNLISRLTSPLSRLKFSKKKATALAAAVVILAFGAYYLIKN 170
           T  N +GKPKN ISRL SPLSRLKFSK+ ATA+A AVVILA G YYLIKN
Sbjct: 121 TQSNGNGKPKNFISRLKSPLSRLKFSKETATAMAVAVVILAVGVYYLIKN 168

BLAST of Carg04737 vs. ExPASy TrEMBL
Match: A0A0A0LT66 (SHSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G031780 PE=3 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 7.7e-57
Identity = 123/170 (72.35%), Postives = 139/170 (81.76%), Query Frame = 0

Query: 1   MEKGEASNLSYEEFEPFCKWKKLDDSDVHVLEVHLPEFKKEELRVRIKNNSILAISGEHL 60
           MEKGE S++ Y +FEP+C+WKK++DSD  VLEV LPEFKKEELRVRIKNNSIL ISGE L
Sbjct: 1   MEKGEPSDVLYVKFEPYCQWKKIEDSD--VLEVQLPEFKKEELRVRIKNNSILTISGEQL 60

Query: 61  -ANDGKKMQFNREIKLPKDIYPDQIRAKFGGSVLSITMPKKPSSAESSKPKADAASEGND 120
            A DGKKM FNR+IKLPKD++PD+IRAKFGG++LSITMPKK S  E SKP  D ASE   
Sbjct: 61  AAKDGKKMHFNRDIKLPKDVFPDEIRAKFGGNILSITMPKKASPPEISKPNPDNASEDKL 120

Query: 121 TAPNDDGKPKNLISRLTSPLSRLKFSKKKATALAAAVVILAFGAYYLIKN 170
           T  N +GKPKN IS+L SPLSRLKFSK  ATA+A AVVILA G YYLIKN
Sbjct: 121 TQSNGNGKPKNFISKLKSPLSRLKFSKATATAMATAVVILAVGVYYLIKN 168

BLAST of Carg04737 vs. TAIR 10
Match: AT1G54400.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 83.2 bits (204), Expect = 2.2e-16
Identity = 57/163 (34.97%), Postives = 86/163 (52.76%), Query Frame = 0

Query: 11  YEEFEPFCKWKKLDDSDVHVLEVHLPE-FKKEELRVRIKNNSILAISGEHLANDGKKMQF 70
           Y+E EPFC+W++ +D D  +LE+HLP   KKE L+++I N+ +L I+G    +  K ++F
Sbjct: 13  YDEIEPFCRWRRTEDID--ILELHLPSGLKKEHLKIQINNSGVLTITGGCPVDQTKTIRF 72

Query: 71  NREIKLPKDIYPDQIRAKFGGSVLSITMPKKPSSAESSKPKADAASEGNDTAPNDDG--K 130
            +E K+ KD   ++IRAKF   VL +TMPK    A         A+  + T   D+G   
Sbjct: 73  MKETKVAKDCKRNEIRAKFSKGVLYVTMPKTIPIAAGLSVSLKGATPQHQTPKTDEGMRN 132

Query: 131 PKNLISRLTSPLSRLK--FSKKKATALAAAVVILAFGAYYLIK 169
             N  S   S    LK    +K      AAVV++A     ++K
Sbjct: 133 VANCSSEFYSKFGSLKQRLWRKTVVEGVAAVVVVAAAVLGVVK 173

BLAST of Carg04737 vs. TAIR 10
Match: AT1G07400.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 64.3 bits (155), Expect = 1.1e-10
Identity = 39/107 (36.45%), Postives = 63/107 (58.88%), Query Frame = 0

Query: 20  WKKLDDSDVHVLEVHLPEFKKEELRVRIKNNSILAISGE-HLANDGKK----------MQ 79
           WK  + ++ HV +  LP  KKEE++V I+++S+L ISGE H+  + K+           Q
Sbjct: 52  WK--ETAEAHVFKADLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGQ 111

Query: 80  FNREIKLPKDIYPDQIRAKFGGSVLSITMPKKPSSAESSKPKADAAS 116
           F+R+ KLP+++  DQ++A     VL++T+PK     E +K KA   S
Sbjct: 112 FSRKFKLPENVKMDQVKASMENGVLTVTVPK----VEEAKKKAQVKS 152

BLAST of Carg04737 vs. TAIR 10
Match: AT2G27140.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 62.8 bits (151), Expect = 3.1e-10
Identity = 37/128 (28.91%), Postives = 62/128 (48.44%), Query Frame = 0

Query: 7   SNLSYEEFEPFCKWKKLDDSDVHVLEVHLPEFKKEELRVRIKNNSILAISGEHLANDGKK 66
           +N  Y+EFEP   WK   +     L ++LP F+KE+L+V++     L + G+  A   K 
Sbjct: 10  ANRIYDEFEPLSNWK--TEQGFEALTIYLPGFRKEQLKVQVTTTRKLRVMGDRPAGANKW 69

Query: 67  MQFNREIKLPKDIYPDQIRAKFGGSVLSITMPKKPSSAESSKP-----KADAASEGNDTA 126
           ++F +E  +P +I  D + AKF G+ L + +P+     +   P     K     + N   
Sbjct: 70  IRFRKEFPIPPNIDVDSVSAKFEGANLVVRLPRTEPMGKQPSPIGTATKPPPVPKENPNL 129

Query: 127 PNDDGKPK 130
           P+   K K
Sbjct: 130 PSPSAKEK 135

BLAST of Carg04737 vs. TAIR 10
Match: AT1G59860.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 61.6 bits (148), Expect = 7.0e-10
Identity = 36/107 (33.64%), Postives = 60/107 (56.07%), Query Frame = 0

Query: 20  WKKLDDSDVHVLEVHLPEFKKEELRVRIKNNSILAISGEHLANDGKKMQ----------- 79
           WK  + ++ HV +  LP  KKEE++V I+++S+L ISGE      +K             
Sbjct: 50  WK--ETAEAHVFKADLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGG 109

Query: 80  FNREIKLPKDIYPDQIRAKFGGSVLSITMPKKPSSAESSKPKADAAS 116
           F+R+ +LP+++  DQ++A     VL++T+PK     E++K KA   S
Sbjct: 110 FSRKFRLPENVKMDQVKASMENGVLTVTVPK----VETNKKKAQVKS 150

BLAST of Carg04737 vs. TAIR 10
Match: AT5G20970.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 60.1 bits (144), Expect = 2.0e-09
Identity = 38/125 (30.40%), Postives = 62/125 (49.60%), Query Frame = 0

Query: 11  YEEFEPFCKWKKLDDSDVHVLEVHLPEFKKEELRVRIKNNSILAISGEHLANDGKKMQFN 70
           Y+EFEP  +W    + D  VL   LP FKKE+L+V +     L ++GE      K ++F+
Sbjct: 10  YQEFEPATRW--TSEPDAEVLVADLPGFKKEQLKVSVTATRKLRLTGERPTGGNKWIRFH 69

Query: 71  REIKLPKDIYPDQIRAKFGGSVLSITMPKKPSSAESSKPKADAASEGNDTAPNDDGK-PK 130
           +EI +P  +  D + A F  + L I  PK  +    +KP      + +D      G+ PK
Sbjct: 70  QEIPVPLTVDIDSVSAMFKDNKLYIRHPKLKTEIPQTKPPTPVIMKPHDQHERKQGQGPK 129

Query: 131 NLISR 135
            ++ +
Sbjct: 130 AMVEK 132

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7020019.11.0e-87100.0017.8 kDa class I heat shock protein [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022923784.12.3e-8799.4217.8 kDa class I heat shock protein-like [Cucurbita moschata] >KAG6584432.1 17.8... [more]
XP_023520341.13.3e-8698.2517.8 kDa class I heat shock protein-like [Cucurbita pepo subsp. pepo][more]
XP_023001660.16.9e-8495.9317.8 kDa class I heat shock protein-like [Cucurbita maxima][more]
XP_008465577.15.7e-6278.82PREDICTED: 16.9 kDa class I heat shock protein 1 [Cucumis melo] >KAA0036046.1 16... [more]
Match NameE-valueIdentityDescription
Q9LNW01.5e-0936.4517.8 kDa class I heat shock protein OS=Arabidopsis thaliana OX=3702 GN=HSP17.8 P... [more]
Q9XIE39.9e-0933.6417.6 kDa class I heat shock protein 1 OS=Arabidopsis thaliana OX=3702 GN=HSP17.6... [more]
Q9ZW314.9e-0836.9017.6 kDa class I heat shock protein 2 OS=Arabidopsis thaliana OX=3702 GN=HSP17.6... [more]
P316732.4e-0731.6817.4 kDa class I heat shock protein OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
Q84J503.2e-0733.6617.7 kDa class I heat shock protein OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
Match NameE-valueIdentityDescription
A0A6J1E7D11.1e-8799.4217.8 kDa class I heat shock protein-like OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1KLT13.3e-8495.9317.8 kDa class I heat shock protein-like OS=Cucurbita maxima OX=3661 GN=LOC11149... [more]
A0A5D3BHU72.7e-6278.8216.9 kDa class I heat shock protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3CP712.7e-6278.8216.9 kDa class I heat shock protein 1 OS=Cucumis melo OX=3656 GN=LOC103502934 PE... [more]
A0A0A0LT667.7e-5772.35SHSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G031780 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT1G54400.12.2e-1634.97HSP20-like chaperones superfamily protein [more]
AT1G07400.11.1e-1036.45HSP20-like chaperones superfamily protein [more]
AT2G27140.13.1e-1028.91HSP20-like chaperones superfamily protein [more]
AT1G59860.17.0e-1033.64HSP20-like chaperones superfamily protein [more]
AT5G20970.12.0e-0930.40HSP20-like chaperones superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008978HSP20-like chaperoneGENE3D2.60.40.790coord: 8..125
e-value: 6.7E-18
score: 66.6
IPR008978HSP20-like chaperoneSUPERFAMILY49764HSP20-like chaperonescoord: 18..103
IPR002068Alpha crystallin/Hsp20 domainPFAMPF00011HSP20coord: 24..107
e-value: 3.5E-12
score: 46.4
IPR002068Alpha crystallin/Hsp20 domainPROSITEPS01031SHSPcoord: 8..116
score: 18.993708
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 97..132
NoneNo IPR availablePANTHERPTHR43670:SF10HSP20-LIKE CHAPERONES SUPERFAMILY PROTEINcoord: 7..168
NoneNo IPR availableCDDcd06464ACD_sHsps-likecoord: 24..99
e-value: 1.44396E-16
score: 68.7348
IPR045045Small heat shock protein RTM2-likePANTHERPTHR43670HEAT SHOCK PROTEIN 26coord: 7..168

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg04737-RACarg04737-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane