Carg04512 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg04512
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Methionine--tRNA ligase
Location	Carg_Chr04: 20071294 .. 20075789 (+)
RNA-Seq Expression	Carg04512
Synteny	Carg04512

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCGCTAGAATAAACACCTCCATACAAAATTCTCTCTGGCTCTTAAACCCTTTGCAGAGTTCTCGCAATCTCAAAGCTACCCATTTGAGGATCCGCTTCAATTTCTATCAAAATCTGTTATCGGCCCCGTCTTCTTGTTCTTCTTCTTGTTCTTCTTCTTCTTCAAAACGAGCCATATTCTGTTCCTCTGCGAGCAATTTCAATTCCCACGATGGCGGAGCCGCTACAGACCCGTTTGTCATCACAACACCACTTTATTATGTTAATGCTGCCCCTCATATGGGGAGTGCTTATACCACCATTGCTGCCGATGCCATTGCGCGGTTTCAGGTTCCTTTTTCCCCTTATTCGGAATCTCCATACTTGTAGTTCTTGTTGGTTGTTCGTTTAGTTAGTGCTTTGGTAACTAGTGGGGCATCTGTTTAATGCTTGATTTGAATGAGCTATGAGTAATTGTTGCTCTTATCGATTATTGGGTGTTCTTTGGTCGGCACTTTGTAGCTGAAATTGAAGATGTTGTTATCATACTTGCCTTCTCCATTTGTTTTCTTTGTATGCTCATATGTAGTCCAGCCAATGTCTGAGAGCATGATCGTTTATGTGAGATCCTACATCGGTTGGAGAGGAGAACAAAACATTCTTGATAAGAATGTGGAAACCTCTCTTAGTAGACGCGTTTTAAAAACCTTGAGGGGAAGCCCGAAAGGGAAAATCCCAAAGAGAATAATATTTGCTAGTGGTGGACTTAGGCCATCAGAGCCAGATAACGGGCGATGTACCAGCGAGGAGGTTGAGCCTTGAAGGGGGTGGACATGCAGCGGTGTGCCAGCAACAACGCTGGGCCCCGAAGAGGGGTGGATTGTGAGATCTCACATCGGTTGGGGATGAGAACGAAGCATTCTTTATAAGAGTGTGAAAACCTCTCCTTAGCAAATGCGTTTTAAAAACCTTGAGGGGAATCCCCGAAAGGAAAAATTCCAAAAAGGACAATATCTGCTAGCGGTGGGCTTGGGCCGTTACGGTTTAGGGATTCATTTTACGCACCTCTCTCTTTCTGATACCTGCTGTTTTAGTTTGTTAATATTTGAATTTTGGAATACAATTTTACCATCAATGGCTCCTCAATGAACTTAAAATTTCAACCCCTTGTGTCAAATATAATAACAGAGGCTGCTAGGAAAGAAAGTCATCTTCATTACTGGCACAGACGAGCACGGGGAGAAGATTGCCACGGCTGCTGCTGCTCAGGGTTCGGGTCCAAGCGAACATTGTGATAACATTTCAGAGGCATACAAAACACTCTGGAAAGATGTAATCCAAACACATTCCATGCTTTTCTTCTCAGTTTGCTGTAATTAGTTTATCAAATATTTAACATTATTCATTATATCTTATTTTTTTTCCTTCTGGTTTCCCCCCTTTGCAGCTAGATATAGCTTATGATAAGTTCATTCGGACAACTGATCCTAAACACGAGACCATTGTAAAGGAATTTTACTCGAACGTTCTTGCCAACGGAGACATCTATAGAGCTGATTATGAGGGACTTTATTGTGTCAATTGTGAGGAATACAAGGTGTTCTTTCTTGTCTTTCTCCTTCTACCCATTTGAAAACTCTGTATTTCGAATCATACGGTATTCACACGTCAAATTTCATGTTTACGATTATCAATTATTCTCTCCTCAGTTATGATAAAATATCGATCGATCATCGCTTTTCCAATAGTCTGCATTAGTTCTCTTACATTTATAAGCACATTCATTTCTGGCCCCGCAGGATGAAAAAGAACTTCTTGCTGACAACTGTTGTCCTATACACCTGAAGCCCTGTGCTTCAAGGAAAGAAGACAATTACTTTTTTGCATTGTCAAAATACCAGAGATTGCTTGAAGAAACTTTAACACAGAATCCGGGGTTCGTGCAGCCTGCCTTCCGTTTGAACGAGGTGACTATAGGCGATTTTTCTACGTTTATGTGTGAAATTTATTATTCATCTTCCATTATAAGTATTGCATTAGATACTATCTAGCTATTGACATTCTAGATTCTCCATTACCAGTAAAGCTAAATGTAAGTCTCGTAAACGCTCATCATATCTCGTTGATCTAATTTGATATTGTATTTATATAGCCATTTTTTTTCTCGAACGACCGAATACCAATTCTATTGTTCGTTACAATCTTTTCCACCTTGCCGATCTCGAACCATCCTTCGTTTAATTCAGCACTATATCTTCCAAGGTGCAAAGTTGGATCAAAAGTGGCCTAAAGGACTTCTCTATCTCCAGAGCATCAGTTGAATGGGGCATTCCAGTTCCCAATGATCAAAAGCAAACAATTTATGTTTGGTTTGATGCATTATTAGGGTAATCTCTTCCCTTATGTTCTTTGTAACCTCGTGTATTATGACTTTTTGAACCTTTATTAAATTTTTTATTACTACAAACGTCTGGTTTATGTCAATTTGCAACAATTCTCCACCATTTTCTAAGAAAATTGAAATGGATGCTACAGCCATTGCCTTCAATCTTAATCATATGAGAATTCCATAATGTAGTTATATATCAGCACTATCAGAGAACGACGAGCAACCAAATTTACAAACGGCCGTTGCTTCGGGTTGGCCTGCTTCTTTACATTTGATTGGTAAGGTAAGAGATACATTCCTGTTATTCAACCATTAATGAACTCAAAATCCTGTGTTATTGTTGTATTCTGGAGGTGCTAATGGTTGTTCCCTTTTCATTTTCTATCTAGCTCAAGCTCGGTTGGTTGGATGCTGATCTAATGTCTTTGTTATTCTTTTCTTTTAGGATATTTTACGTTTCCATGCAGTTTATTGGCCTGCTATGCTAATGTCTGCTGGACTAAAGCTTCCAAAGATGGTTTTTGGTCATGGGTTCTTGACGAAGGTATGTTTGGCCCCCTAAAGTTGCTACCGACAAAGGAATTTAGTTGGCGACTGTACCGGTCTAACGCTAAGGACGTAGAAGAGAGTTAATATTTTATTAAGTCTGCAAAGACGAAACTAGTCACCGTTACGTTATGTATAATGTTAATATTGATGTATCTTGTGAATTATACTAGTCACCATTACTTTAGTCTTCTGGTTCAAGCTTCTTTGTGAATCTGGTGTATTTTGGTTCCTAAACTTTATTTCATGCACCAACTCCTTTTGTTTTGACTTTATAGGATGGTATGAAGATGGGCAAGTCGTTAGGCAATACTCTTGAACCAAATGAATTGGTTCAAAAGTTTGGCTCGGACCCCGTGCGATACTTCTTCCTTCGAGAGGTGGAATTTGGTAGTGATGGAGACTATGCTGAGGATCGTTTCATCAATATTGTCAATGCGCATCTTGCAAATACAATAGGTATATTTTAACTGGAATCCCTATTGAACGGAAAATTACAGCCCTTTTTAATGGAGGCTTCTGTTCGACACCATGTAATCTTGACGTTTCTTTTGCAGGAAATCTTCTTAATCGAACTCTCGGACTCCTTAAAAAGAACTGCCAATCTACTTTGGTGGTTGATTCGTCTGCTGCAGCTGTAGGAAATGCTTTCAAGGAAAAAGTCGAGGAATTGGTAAGGTCGACTTGGAAATTACATTGGTATTTGTTATCTGAGATGGCACGGAGTTGGCGAAATCATTCTATATCGATTTTTTGGTCGTTGAAATCTTCTCTAGGATCAATTCAGTAAATAGCTCAGTCACTGAGTCCACTGTGGCGTGGATATTGTCTGTTGTGTGTCCTCTGTTTATGACTTTATGACCAATAAGTTCACGAGTATTTGCACCGTGTCGATTTTATTAGTCTGCCTGGTAGGTTGCGAAAGCTCGGAATCATTACGAAGAGCTCTCCCTATCATCGGCTTGTGAGGCTGTGTTGGAGATTGGCAATGCTGGAAATTCCTACATGGATGAGCGAGCACCATGGTCTCTCTTCAAACAAGGGGGTGCCTCATCAGACGCAGCTGCAAAGGTTATCCAGTTCAAGAATCCATTTTTGTTTATGCGCATGATTTTCGATATGAATTTTCGATTTACGATTCCTTCCAACATTCAACAACCCCTTCTCTGAATTCAATCTGGCTCAATTATCTTGACAGGACCTTGTTCTTATACTCGAGGCGATGCGAATCATAGCCATTGCACTATCCCCAGTAACGCCACGCTTAAGCTGGAGAATATACGAGCAGCTCGGCTACCCTGAGGACCAGTTTGCAGCTGCAACATGGGTCTTATACTCTTTTCATACTTGTGTATTGTTCTTTTCTTGATATCTCAGCTCATTACCAGTGACTGATAATGTGACTTTTTGGGTGTAGAATGAGACGAAATGGGGAGGGCTGAAGGCTGGTCAGGTTATGGCTCCTCCAAACCCAGTCTTTGCTAGGATTGAACAAGCCATTGTTAATGGTGAAGAAGAAGCTCCCAAAAAACCAGTCAAAAGCAAGAAGAAAATAGCTCAAAAAGTGGCAGAAGCCTAA

mRNA sequence

ATGGCCGCTAGAATAAACACCTCCATACAAAATTCTCTCTGGCTCTTAAACCCTTTGCAGAGTTCTCGCAATCTCAAAGCTACCCATTTGAGGATCCGCTTCAATTTCTATCAAAATCTGTTATCGGCCCCGTCTTCTTGTTCTTCTTCTTGTTCTTCTTCTTCTTCAAAACGAGCCATATTCTGTTCCTCTGCGAGCAATTTCAATTCCCACGATGGCGGAGCCGCTACAGACCCGTTTGTCATCACAACACCACTTTATTATGTTAATGCTGCCCCTCATATGGGGAGTGCTTATACCACCATTGCTGCCGATGCCATTGCGCGGTTTCAGAGGCTGCTAGGAAAGAAAGTCATCTTCATTACTGGCACAGACGAGCACGGGGAGAAGATTGCCACGGCTGCTGCTGCTCAGGGTTCGGGTCCAAGCGAACATTGTGATAACATTTCAGAGGCATACAAAACACTCTGGAAAGATCTAGATATAGCTTATGATAAGTTCATTCGGACAACTGATCCTAAACACGAGACCATTGTAAAGGAATTTTACTCGAACGTTCTTGCCAACGGAGACATCTATAGAGCTGATTATGAGGGACTTTATTGTGTCAATTGTGAGGAATACAAGGATGAAAAAGAACTTCTTGCTGACAACTGTTGTCCTATACACCTGAAGCCCTGTGCTTCAAGGAAAGAAGACAATTACTTTTTTGCATTGTCAAAATACCAGAGATTGCTTGAAGAAACTTTAACACAGAATCCGGGGTTCGTGCAGCCTGCCTTCCGTTTGAACGAGGTGCAAAGTTGGATCAAAAGTGGCCTAAAGGACTTCTCTATCTCCAGAGCATCAGTTGAATGGGGCATTCCAGTTCCCAATGATCAAAAGCAAACAATTTATGTTTGGTTTGATGCATTATTAGGTTATATATCAGCACTATCAGAGAACGACGAGCAACCAAATTTACAAACGGCCGTTGCTTCGGGTTGGCCTGCTTCTTTACATTTGATTGGTAAGGATATTTTACGTTTCCATGCAGTTTATTGGCCTGCTATGCTAATGTCTGCTGGACTAAAGCTTCCAAAGATGGTTTTTGGTCATGGGTTCTTGACGAAGGATGGTATGAAGATGGGCAAGTCGTTAGGCAATACTCTTGAACCAAATGAATTGGTTCAAAAGTTTGGCTCGGACCCCGTGCGATACTTCTTCCTTCGAGAGGTGGAATTTGGTAGTGATGGAGACTATGCTGAGGATCGTTTCATCAATATTGTCAATGCGCATCTTGCAAATACAATAGGAAATCTTCTTAATCGAACTCTCGGACTCCTTAAAAAGAACTGCCAATCTACTTTGGTGGTTGATTCGTCTGCTGCAGCTGTAGGAAATGCTTTCAAGGAAAAAGTCGAGGAATTGGTTGCGAAAGCTCGGAATCATTACGAAGAGCTCTCCCTATCATCGGCTTGTGAGGCTGTGTTGGAGATTGGCAATGCTGGAAATTCCTACATGGATGAGCGAGCACCATGGTCTCTCTTCAAACAAGGGGGTGCCTCATCAGACGCAGCTGCAAAGGACCTTGTTCTTATACTCGAGGCGATGCGAATCATAGCCATTGCACTATCCCCAGTAACGCCACGCTTAAGCTGGAGAATATACGAGCAGCTCGGCTACCCTGAGGACCAGTTTGCAGCTGCAACATGGAATGAGACGAAATGGGGAGGGCTGAAGGCTGGTCAGGTTATGGCTCCTCCAAACCCAGTCTTTGCTAGGATTGAACAAGCCATTGTTAATGGTGAAGAAGAAGCTCCCAAAAAACCAGTCAAAAGCAAGAAGAAAATAGCTCAAAAAGTGGCAGAAGCCTAA

Coding sequence (CDS)

ATGGCCGCTAGAATAAACACCTCCATACAAAATTCTCTCTGGCTCTTAAACCCTTTGCAGAGTTCTCGCAATCTCAAAGCTACCCATTTGAGGATCCGCTTCAATTTCTATCAAAATCTGTTATCGGCCCCGTCTTCTTGTTCTTCTTCTTGTTCTTCTTCTTCTTCAAAACGAGCCATATTCTGTTCCTCTGCGAGCAATTTCAATTCCCACGATGGCGGAGCCGCTACAGACCCGTTTGTCATCACAACACCACTTTATTATGTTAATGCTGCCCCTCATATGGGGAGTGCTTATACCACCATTGCTGCCGATGCCATTGCGCGGTTTCAGAGGCTGCTAGGAAAGAAAGTCATCTTCATTACTGGCACAGACGAGCACGGGGAGAAGATTGCCACGGCTGCTGCTGCTCAGGGTTCGGGTCCAAGCGAACATTGTGATAACATTTCAGAGGCATACAAAACACTCTGGAAAGATCTAGATATAGCTTATGATAAGTTCATTCGGACAACTGATCCTAAACACGAGACCATTGTAAAGGAATTTTACTCGAACGTTCTTGCCAACGGAGACATCTATAGAGCTGATTATGAGGGACTTTATTGTGTCAATTGTGAGGAATACAAGGATGAAAAAGAACTTCTTGCTGACAACTGTTGTCCTATACACCTGAAGCCCTGTGCTTCAAGGAAAGAAGACAATTACTTTTTTGCATTGTCAAAATACCAGAGATTGCTTGAAGAAACTTTAACACAGAATCCGGGGTTCGTGCAGCCTGCCTTCCGTTTGAACGAGGTGCAAAGTTGGATCAAAAGTGGCCTAAAGGACTTCTCTATCTCCAGAGCATCAGTTGAATGGGGCATTCCAGTTCCCAATGATCAAAAGCAAACAATTTATGTTTGGTTTGATGCATTATTAGGTTATATATCAGCACTATCAGAGAACGACGAGCAACCAAATTTACAAACGGCCGTTGCTTCGGGTTGGCCTGCTTCTTTACATTTGATTGGTAAGGATATTTTACGTTTCCATGCAGTTTATTGGCCTGCTATGCTAATGTCTGCTGGACTAAAGCTTCCAAAGATGGTTTTTGGTCATGGGTTCTTGACGAAGGATGGTATGAAGATGGGCAAGTCGTTAGGCAATACTCTTGAACCAAATGAATTGGTTCAAAAGTTTGGCTCGGACCCCGTGCGATACTTCTTCCTTCGAGAGGTGGAATTTGGTAGTGATGGAGACTATGCTGAGGATCGTTTCATCAATATTGTCAATGCGCATCTTGCAAATACAATAGGAAATCTTCTTAATCGAACTCTCGGACTCCTTAAAAAGAACTGCCAATCTACTTTGGTGGTTGATTCGTCTGCTGCAGCTGTAGGAAATGCTTTCAAGGAAAAAGTCGAGGAATTGGTTGCGAAAGCTCGGAATCATTACGAAGAGCTCTCCCTATCATCGGCTTGTGAGGCTGTGTTGGAGATTGGCAATGCTGGAAATTCCTACATGGATGAGCGAGCACCATGGTCTCTCTTCAAACAAGGGGGTGCCTCATCAGACGCAGCTGCAAAGGACCTTGTTCTTATACTCGAGGCGATGCGAATCATAGCCATTGCACTATCCCCAGTAACGCCACGCTTAAGCTGGAGAATATACGAGCAGCTCGGCTACCCTGAGGACCAGTTTGCAGCTGCAACATGGAATGAGACGAAATGGGGAGGGCTGAAGGCTGGTCAGGTTATGGCTCCTCCAAACCCAGTCTTTGCTAGGATTGAACAAGCCATTGTTAATGGTGAAGAAGAAGCTCCCAAAAAACCAGTCAAAAGCAAGAAGAAAATAGCTCAAAAAGTGGCAGAAGCCTAA

Protein sequence

MAARINTSIQNSLWLLNPLQSSRNLKATHLRIRFNFYQNLLSAPSSCSSSCSSSSSKRAIFCSSASNFNSHDGGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITGTDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVKEFYSNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSKYQRLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQKQTIYVWFDALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFINIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEELSLSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSPVTPRLSWRIYEQLGYPEDQFAAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEAPKKPVKSKKKIAQKVAEA

Homology

[Close]

BLAST of Carg04512 vs. NCBI nr
Match: KAG7033041.1 (Methionine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1234.9 bits (3194), Expect = 0.0e+00
Identity = 618/618 (100.00%), Postives = 618/618 (100.00%), Query Frame = 0


Query: 1   MAARINTSIQNSLWLLNPLQSSRNLKATHLRIRFNFYQNLLSAPSSCSSSCSSSSSKRAI 60
           MAARINTSIQNSLWLLNPLQSSRNLKATHLRIRFNFYQNLLSAPSSCSSSCSSSSSKRAI
Sbjct: 1   MAARINTSIQNSLWLLNPLQSSRNLKATHLRIRFNFYQNLLSAPSSCSSSCSSSSSKRAI 60

Query: 61  FCSSASNFNSHDGGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 120
           FCSSASNFNSHDGGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF
Sbjct: 61  FCSSASNFNSHDGGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 120

Query: 121 ITGTDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVK 180
           ITGTDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVK
Sbjct: 121 ITGTDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVK 180

Query: 181 EFYSNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS 240
           EFYSNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS
Sbjct: 181 EFYSNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS 240

Query: 241 KYQRLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQKQTIYV 300
           KYQRLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQKQTIYV
Sbjct: 241 KYQRLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQKQTIYV 300

Query: 301 WFDALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 360
           WFDALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP
Sbjct: 301 WFDALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 360

Query: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFI 420
           KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFI
Sbjct: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFI 420

Query: 421 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEE 480
           NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEE
Sbjct: 421 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEE 480

Query: 481 LSLSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSP 540
           LSLSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSP
Sbjct: 481 LSLSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSP 540

Query: 541 VTPRLSWRIYEQLGYPEDQFAAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEA 600
           VTPRLSWRIYEQLGYPEDQFAAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEA
Sbjct: 541 VTPRLSWRIYEQLGYPEDQFAAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEA 600

Query: 601 PKKPVKSKKKIAQKVAEA 619
           PKKPVKSKKKIAQKVAEA
Sbjct: 601 PKKPVKSKKKIAQKVAEA 618

[Close]

BLAST of Carg04512 vs. NCBI nr
Match: XP_022960433.1 (methionine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita moschata])

HSP 1 Score: 1220.7 bits (3157), Expect = 0.0e+00
Identity = 613/618 (99.19%), Postives = 613/618 (99.19%), Query Frame = 0


Query: 1   MAARINTSIQNSLWLLNPLQSSRNLKATHLRIRFNFYQNLLSAPSSCSSSCSSSSSKRAI 60
           MAARINTSIQNSLWLLNPLQSSRNLKATHLR RFNFYQNLLSAP    SSCSSSSSKRAI
Sbjct: 1   MAARINTSIQNSLWLLNPLQSSRNLKATHLRTRFNFYQNLLSAP----SSCSSSSSKRAI 60

Query: 61  FCSSASNFNSHDGGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 120
           FCSSASNFNSHDGGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF
Sbjct: 61  FCSSASNFNSHDGGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 120

Query: 121 ITGTDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVK 180
           ITGTDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVK
Sbjct: 121 ITGTDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVK 180

Query: 181 EFYSNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS 240
           EFYSNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS
Sbjct: 181 EFYSNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS 240

Query: 241 KYQRLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQKQTIYV 300
           KYQRLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQKQTIYV
Sbjct: 241 KYQRLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQKQTIYV 300

Query: 301 WFDALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 360
           WFDALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP
Sbjct: 301 WFDALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 360

Query: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFI 420
           KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFI
Sbjct: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFI 420

Query: 421 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEE 480
           NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEE
Sbjct: 421 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEE 480

Query: 481 LSLSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSP 540
           LSLSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSP
Sbjct: 481 LSLSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSP 540

Query: 541 VTPRLSWRIYEQLGYPEDQFAAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEA 600
           VTPRLSWRIYEQLGYPEDQFAAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEA
Sbjct: 541 VTPRLSWRIYEQLGYPEDQFAAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEA 600

Query: 601 PKKPVKSKKKIAQKVAEA 619
           PKKPVKSKKKIAQKVAEA
Sbjct: 601 PKKPVKSKKKIAQKVAEA 614

[Close]

BLAST of Carg04512 vs. NCBI nr
Match: KAG6602362.1 (Methionine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1217.2 bits (3148), Expect = 0.0e+00
Identity = 611/618 (98.87%), Postives = 612/618 (99.03%), Query Frame = 0


Query: 1   MAARINTSIQNSLWLLNPLQSSRNLKATHLRIRFNFYQNLLSAPSSCSSSCSSSSSKRAI 60
           MAARINTSIQNSLWLLNPLQSSRNLKATHLR RF FYQNLLSAP    SSCSSSSSKRAI
Sbjct: 1   MAARINTSIQNSLWLLNPLQSSRNLKATHLRTRFKFYQNLLSAP----SSCSSSSSKRAI 60

Query: 61  FCSSASNFNSHDGGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 120
           FCSSASNFNSHDGGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF
Sbjct: 61  FCSSASNFNSHDGGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 120

Query: 121 ITGTDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVK 180
           ITGTDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVK
Sbjct: 121 ITGTDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVK 180

Query: 181 EFYSNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS 240
           EFYSNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS
Sbjct: 181 EFYSNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS 240

Query: 241 KYQRLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQKQTIYV 300
           KYQRLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQKQTIYV
Sbjct: 241 KYQRLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQKQTIYV 300

Query: 301 WFDALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 360
           WFDALLGYISALSENDEQPNLQTAVASGWPAS+HLIGKDILRFHAVYWPAMLMSAGLKLP
Sbjct: 301 WFDALLGYISALSENDEQPNLQTAVASGWPASVHLIGKDILRFHAVYWPAMLMSAGLKLP 360

Query: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFI 420
           KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFI
Sbjct: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFI 420

Query: 421 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEE 480
           NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEE
Sbjct: 421 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEE 480

Query: 481 LSLSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSP 540
           LSLSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSP
Sbjct: 481 LSLSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSP 540

Query: 541 VTPRLSWRIYEQLGYPEDQFAAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEA 600
           VTPRLSWRIYEQLGYPEDQFAAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEA
Sbjct: 541 VTPRLSWRIYEQLGYPEDQFAAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEA 600

Query: 601 PKKPVKSKKKIAQKVAEA 619
           PKKPVKSKKKIAQKVAEA
Sbjct: 601 PKKPVKSKKKIAQKVAEA 614

[Close]

BLAST of Carg04512 vs. NCBI nr
Match: XP_023538063.1 (methionine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1213.4 bits (3138), Expect = 0.0e+00
Identity = 608/618 (98.38%), Postives = 611/618 (98.87%), Query Frame = 0


Query: 1   MAARINTSIQNSLWLLNPLQSSRNLKATHLRIRFNFYQNLLSAPSSCSSSCSSSSSKRAI 60
           MAARINTSIQNSLWLLNPLQ+SRNLKATH R RF FYQNLLSAP    SSCSSSSSKRA+
Sbjct: 1   MAARINTSIQNSLWLLNPLQNSRNLKATHFRTRFKFYQNLLSAP----SSCSSSSSKRAL 60

Query: 61  FCSSASNFNSHDGGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 120
           FCSSASNFNSHDGGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF
Sbjct: 61  FCSSASNFNSHDGGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 120

Query: 121 ITGTDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVK 180
           ITGTDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVK
Sbjct: 121 ITGTDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVK 180

Query: 181 EFYSNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS 240
           EFYSNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS
Sbjct: 181 EFYSNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS 240

Query: 241 KYQRLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQKQTIYV 300
           KYQRLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQKQTIYV
Sbjct: 241 KYQRLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQKQTIYV 300

Query: 301 WFDALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 360
           WFDALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP
Sbjct: 301 WFDALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 360

Query: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFI 420
           KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFI
Sbjct: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFI 420

Query: 421 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEE 480
           NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEE
Sbjct: 421 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEE 480

Query: 481 LSLSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSP 540
           LSLSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSP
Sbjct: 481 LSLSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSP 540

Query: 541 VTPRLSWRIYEQLGYPEDQFAAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEA 600
           VTPRLSWRIYEQLG+PEDQFAAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEA
Sbjct: 541 VTPRLSWRIYEQLGFPEDQFAAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEA 600

Query: 601 PKKPVKSKKKIAQKVAEA 619
           PKKPVKSKKKIAQKVAEA
Sbjct: 601 PKKPVKSKKKIAQKVAEA 614

[Close]

BLAST of Carg04512 vs. NCBI nr
Match: XP_022990077.1 (methionine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita maxima])

HSP 1 Score: 1206.4 bits (3120), Expect = 0.0e+00
Identity = 606/618 (98.06%), Postives = 609/618 (98.54%), Query Frame = 0


Query: 1   MAARINTSIQNSLWLLNPLQSSRNLKATHLRIRFNFYQNLLSAPSSCSSSCSSSSSKRAI 60
           MAARINTSIQNSLWLLNPLQ+SRNLKATHLR RF FYQNLLSAPSSC     SSSSKRAI
Sbjct: 1   MAARINTSIQNSLWLLNPLQNSRNLKATHLRTRFKFYQNLLSAPSSC-----SSSSKRAI 60

Query: 61  FCSSASNFNSHDGGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 120
           FCSSASNFNSHDGGAAT PFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF
Sbjct: 61  FCSSASNFNSHDGGAATGPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 120

Query: 121 ITGTDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVK 180
           ITGTDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVK
Sbjct: 121 ITGTDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVK 180

Query: 181 EFYSNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS 240
           EFYSNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS
Sbjct: 181 EFYSNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS 240

Query: 241 KYQRLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQKQTIYV 300
           KYQRLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQ+QTIYV
Sbjct: 241 KYQRLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQRQTIYV 300

Query: 301 WFDALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 360
           WFDALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP
Sbjct: 301 WFDALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 360

Query: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFI 420
           KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFI
Sbjct: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFI 420

Query: 421 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEE 480
           NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEE
Sbjct: 421 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEE 480

Query: 481 LSLSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSP 540
           LSLSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSP
Sbjct: 481 LSLSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSP 540

Query: 541 VTPRLSWRIYEQLGYPEDQFAAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEA 600
           VTPRLSWRIYEQLG+PEDQFAAATWNETKWGGLKAGQVMA PNPVFARIEQAIVNGEEEA
Sbjct: 541 VTPRLSWRIYEQLGFPEDQFAAATWNETKWGGLKAGQVMALPNPVFARIEQAIVNGEEEA 600

Query: 601 PKKPVKSKKKIAQKVAEA 619
           PKKPVKSKKKIAQKVAEA
Sbjct: 601 PKKPVKSKKKIAQKVAEA 613

[Close]

BLAST of Carg04512 vs. ExPASy Swiss-Prot
Match: Q9M2T9 (Methionine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OVA1 PE=2 SV=1)

HSP 1 Score: 902.1 bits (2330), Expect = 3.4e-261
Identity = 458/616 (74.35%), Postives = 513/616 (83.28%), Query Frame = 0


Query: 1   MAARINTSIQNSLWLLNPLQSSRNLKATHLRIRFNFYQNLLSAPSSCSSSCSSSSSKRAI 60
           MAARINTS+ N+L  L P  +  N K       F+F +N              SS KRA+
Sbjct: 1   MAARINTSLHNALSFLKPFNTPLNTKP------FSFRRNSFRFSKKLPYYSQFSSGKRAL 60

Query: 61  FCSSASNFNSHDGGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 120
           +C+S+S  ++ D G   + FV+TTPLYYVNA PHMGSAYTTIAAD+IARFQRLLGKKVIF
Sbjct: 61  YCTSSSQESTVDEG---ETFVLTTPLYYVNAPPHMGSAYTTIAADSIARFQRLLGKKVIF 120

Query: 121 ITGTDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVK 180
           ITGTDEHGEKIAT+AAA G  P EHCD IS++Y+TLWKDLDIAYDKFIRTTDPKHE IVK
Sbjct: 121 ITGTDEHGEKIATSAAANGRNPPEHCDLISQSYRTLWKDLDIAYDKFIRTTDPKHEAIVK 180

Query: 181 EFYSNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS 240
           EFY+ V ANGDIYRADYEGLYCVNCEEYKDEKELL +NCCP+H  PC +RKEDNYFFALS
Sbjct: 181 EFYARVFANGDIYRADYEGLYCVNCEEYKDEKELLENNCCPVHQMPCVARKEDNYFFALS 240

Query: 241 KYQRLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQKQTIYV 300
           KYQ+ LE+ L QNP FVQP++RLNEVQSWIKSGL+DFSISRA V+WGIPVP+D KQTIYV
Sbjct: 241 KYQKPLEDILAQNPRFVQPSYRLNEVQSWIKSGLRDFSISRALVDWGIPVPDDDKQTIYV 300

Query: 301 WFDALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 360
           WFDALLGYISAL+E+++Q NL+TAV+ GWPASLHLIGKDILRFHAVYWPAMLMSAGL+LP
Sbjct: 301 WFDALLGYISALTEDNKQQNLETAVSFGWPASLHLIGKDILRFHAVYWPAMLMSAGLELP 360

Query: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFI 420
           KMVFGHGFLTKDGMKMGKSLGNTLEP ELVQKFG D VRYFFLREVEFG+DGDY+EDRFI
Sbjct: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPFELVQKFGPDAVRYFFLREVEFGNDGDYSEDRFI 420

Query: 421 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEE 480
            IVNAHLANTIGNLLNRTLGLLKKNC+STLVVDS+ AA G   K+ VE+LV KAR +YE 
Sbjct: 421 KIVNAHLANTIGNLLNRTLGLLKKNCESTLVVDSTVAAEGVPLKDTVEKLVEKARTNYEN 480

Query: 481 LSLSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSP 540
           LSLSSACEAVLEIGNAGN+YMD+RAPW LFKQGG S++ AAKDLV+ILE MR+IA+ALSP
Sbjct: 481 LSLSSACEAVLEIGNAGNTYMDQRAPWFLFKQGGVSAEEAAKDLVIILEVMRVIAVALSP 540

Query: 541 VTPRLSWRIYEQLGYPEDQFAAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGE-EE 600
           V P LS RIY QLGY EDQF + TW++TKWGGLK GQVM   +PVFARIE   +N E EE
Sbjct: 541 VAPCLSLRIYSQLGYTEDQFNSITWSDTKWGGLKGGQVMEQASPVFARIE---LNPEKEE 600

Query: 601 APKKPVKSKKKIAQKV 616
             KKP   KK    KV
Sbjct: 601 DEKKPKVGKKTGKAKV 604

[Close]

BLAST of Carg04512 vs. ExPASy Swiss-Prot
Match: Q55729 (Methionine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=metG PE=3 SV=1)

HSP 1 Score: 571.6 bits (1472), Expect = 1.1e-161
Identity = 278/521 (53.36%), Postives = 356/521 (68.33%), Query Frame = 0


Query: 80  FVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITGTDEHGEKIATAAAAQG 139
           F +TTPLYYVN  PH+GSAYTT+ AD +ARF+RL G  V+ ITGTDEHG+KI   A AQ 
Sbjct: 9   FSVTTPLYYVNDVPHLGSAYTTVVADTLARFKRLQGYDVLMITGTDEHGQKIQRTAEAQE 68

Query: 140 SGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVKEFYSNVLANGDIYRADYEG 199
             P  HCD     +K LW+ L+I YD+F RTTDP+H  IVK+F+  V   GDIY A  +G
Sbjct: 69  LDPQTHCDRTVVKFKELWRSLNILYDRFSRTTDPRHLAIVKDFFQRVWDKGDIYLAQQQG 128

Query: 200 LYCVNCEEYKDEKELLADNCCPIHLKPCAS-RKEDNYFFALSKYQRLLEETLTQNPGFVQ 259
            YCV CEE+K++++LL DN CP+H    A  R E+NYFF LS+YQ  LEE   Q P F+Q
Sbjct: 129 WYCVACEEFKEKRDLLEDNHCPLHPNRKAEWRDEENYFFRLSRYQHPLEELYAQRPEFIQ 188

Query: 260 PAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQKQTIYVWFDALLGYISALSENDEQ 319
           P+ R NEV +++  GL+DFSISR +++WG P+PND   TIYVWFDALLGY++AL + DE+
Sbjct: 189 PSSRRNEVLNFVAQGLQDFSISRVNLDWGFPLPNDPNHTIYVWFDALLGYVTALLDEDEE 248

Query: 320 PNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHGFLTKDGMKMGK 379
           PNL  A+   WP +LHLIGKDILRFHAVYWPAMLMSA L +P  VFGHGFLTKDG KMGK
Sbjct: 249 PNLTNALVKWWPINLHLIGKDILRFHAVYWPAMLMSAELAIPAQVFGHGFLTKDGQKMGK 308

Query: 380 SLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFINIVNAHLANTIGNLLNRT 439
           SLGNT++P +L+ ++G D  RY+FL+E+EFG DGD+ E RF+N++NA LAN +GNLLNRT
Sbjct: 309 SLGNTVDPLDLINRYGEDAFRYYFLKEIEFGKDGDFNEQRFVNVLNADLANDLGNLLNRT 368

Query: 440 LGLLKKNCQST-LVVDSSAAAVGNAFKEKVEELVAKARNHYEELSLSSACEAVLEIGNAG 499
           LG++KK CQ     V ++  A  N  K     L  +  + YE LS + ACEA+  +  AG
Sbjct: 369 LGMVKKYCQGQGPQVMATDLAPDNPLKALGSHLGEEVSSAYERLSFTDACEAIFTLVRAG 428

Query: 500 NSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSPVTPRLSWRIYEQLGYPE 559
           N Y+D+ APW LFKQG  S       L  +LE++R+ A  LSP+ PRLS +IY+QLG+  
Sbjct: 429 NKYIDDMAPWKLFKQG--SQKEVEDVLYSVLESIRLSAYLLSPIVPRLSTKIYQQLGFTW 488

Query: 560 D--------QFAAATWNETKWGGLKAGQVMAPPNPVFARIE 591
           D        + A        WG L A Q + P  P+F ++E
Sbjct: 489 DFDQWRSPLEQAEEFNRHQSWGQLGANQNLPPAQPIFTKLE 527

[Close]

BLAST of Carg04512 vs. ExPASy Swiss-Prot
Match: Q8Z068 (Methionine--tRNA ligase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=metG PE=3 SV=1)

HSP 1 Score: 560.5 bits (1443), Expect = 2.4e-158
Identity = 276/522 (52.87%), Postives = 360/522 (68.97%), Query Frame = 0


Query: 76  ATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITGTDEHGEKIATAA 135
           A   F +TTPLYYVN  PH+GSAYTT+AADA+ARFQ+LLG+ V+ ITGTDEHG+KI  +A
Sbjct: 7   AEKTFALTTPLYYVNDVPHIGSAYTTMAADAVARFQKLLGRDVLLITGTDEHGQKIQRSA 66

Query: 136 AAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVKEFYSNVLANGDIYRA 195
            + G  P E CD I  ++ +LW+ L+I YD+F RTT  +H+ IV EF++ V   GDIY+ 
Sbjct: 67  ESLGKAPQEFCDEIVPSFMSLWRLLNIQYDRFSRTTAVRHKAIVDEFFARVWEAGDIYQG 126

Query: 196 DYEGLYCVNCEEYKDEKELLADNCCPIHL-KPCASRKEDNYFFALSKYQRLLEETLTQNP 255
             +G YCV+CEE+K+E+ELL  N CPIH+ K    R E NYFF LSKYQ  LEE    +P
Sbjct: 127 QQKGWYCVSCEEFKEERELLEGNRCPIHVNKEVEWRDEQNYFFRLSKYQTQLEEFYQSHP 186

Query: 256 GFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQKQTIYVWFDALLGYISALSE 315
            F+QP  R NEV +++  GL+DFSISR +++WG PVPND K T+YVWFDALL Y++AL +
Sbjct: 187 DFIQPESRRNEVLNFVSQGLQDFSISRVNLDWGFPVPNDPKHTLYVWFDALLAYVTALLD 246

Query: 316 NDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHGFLTKDGM 375
            +++P L+ A+   WP +LHLIGKDILRFHAVYWPAML+SAGL LP  VFGHGFLTKDG 
Sbjct: 247 PEDEPTLENALGKWWPINLHLIGKDILRFHAVYWPAMLLSAGLPLPDRVFGHGFLTKDGQ 306

Query: 376 KMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFINIVNAHLANTIGNL 435
           KMGKSLGNT++P  LVQ++GSD VRY+FL+E+EFG DGD+ E RFI+++NA LAN +GNL
Sbjct: 307 KMGKSLGNTVDPVGLVQQYGSDAVRYYFLKEIEFGKDGDFNEVRFIHVLNADLANDLGNL 366

Query: 436 LNRTLGLLKKNCQS-TLVVDSSAAAVGNAFKEKVEELVAKARNHYEELSLSSACEAVLEI 495
           LNRTL ++KK C +  L + +      N  K    +L AK +  YE L+ + AC  VL +
Sbjct: 367 LNRTLNMVKKYCANYALSITNEDIPAENTLKALGVDLGAKVKQAYEVLAFNQACGVVLSL 426

Query: 496 GNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSPVTPRLSWRIYEQL 555
             A N ++D++APWSL+KQ           L  +LE++R+ A  LSPV P +S  IY+QL
Sbjct: 427 VQASNKFIDDQAPWSLYKQ--ERQQEVETVLYTVLESVRLAAYLLSPVIPNISSNIYQQL 486

Query: 556 G----YPEDQFAAATWNETKWGGLKAGQVMAPPNPVFARIEQ 592
           G    + E           +WG L   Q +  P P+F RIEQ
Sbjct: 487 GFGINFNEQTDITPFAIHAQWGLLSNKQQLGQPQPIFKRIEQ 526

[Close]

BLAST of Carg04512 vs. ExPASy Swiss-Prot
Match: P59081 (Methionine--tRNA ligase OS=Thermosynechococcus elongatus (strain BP-1) OX=197221 GN=metG PE=3 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 2.2e-151
Identity = 264/518 (50.97%), Postives = 350/518 (67.57%), Query Frame = 0


Query: 80  FVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITGTDEHGEKIATAAAAQG 139
           F +TTPLYYVNA PH+GSAYTTIAAD +ARF RL G +V FITGTDEHG+KI   A  +G
Sbjct: 5   FSLTTPLYYVNALPHIGSAYTTIAADVLARFYRLQGYQVRFITGTDEHGQKIERTAQQRG 64

Query: 140 SGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVKEFYSNVLANGDIYRADYEG 199
             P  HCD I+  ++ LW+ L+I YD+F RTT P+H  IV EF+  V  NGDIY    +G
Sbjct: 65  LSPQAHCDEIAAGFQALWQQLNIHYDRFSRTTSPRHHAIVNEFFQRVWDNGDIYLGQQQG 124

Query: 200 LYCVNCEEYKDEKELLADNCCPIHLKPCAS-RKEDNYFFALSKYQRLLEETLTQNPGFVQ 259
            YCV CEE+K+E+ELL    CPIH+      R E NYFF LSKYQ+ L +   ++P FVQ
Sbjct: 125 WYCVECEEFKEERELLEGRRCPIHVNRTVEWRDERNYFFRLSKYQQALLDHYAEHPDFVQ 184

Query: 260 PAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQKQTIYVWFDALLGYISALSENDEQ 319
           P  R NEV S+I+ GL+DFSISR ++ WG PVP D +QT+YVWFDALLGY++AL E +++
Sbjct: 185 PPSRRNEVLSFIERGLQDFSISRVNLAWGFPVPTDPEQTLYVWFDALLGYVTALLEPEDE 244

Query: 320 PNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHGFLTKDGMKMGK 379
           P L  A+ + WP +LH+IGKDILRFH + WPAMLMSAGL LP+ +F HGFLTKDG KMGK
Sbjct: 245 PTLANALKTWWPINLHIIGKDILRFHGISWPAMLMSAGLPLPEQIFVHGFLTKDGQKMGK 304

Query: 380 SLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFINIVNAHLANTIGNLLNRT 439
           SLGNTL+P  LV ++G+D VRY+F++EVEFG DGD++E RF+ I+NA LAN +GNLLNRT
Sbjct: 305 SLGNTLDPFALVAQYGADAVRYYFMKEVEFGRDGDFSETRFVTILNADLANDLGNLLNRT 364

Query: 440 LGLLKKNCQSTLV-VDSSAAAVGNAFKEKVEELVAKARNHYEELSLSSACEAVLEIGNAG 499
           L +  K     +  V  +A    +  ++  E L       Y +L+    C+  L +  AG
Sbjct: 365 LKMAWKYTDGKVPNVQGAAIPREHPLRQLAEHLSQTYGQGYRQLAFHEVCQQALTLARAG 424

Query: 500 NSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSPVTPRLSWRIYEQLGYPE 559
           N ++DE APW  ++ G   + A A+ L  +LE++R++A  L+P+ P+LS  IY QLGY  
Sbjct: 425 NKFLDEEAPWKRYRAG--ETAAVAEILYCVLESVRLVAYVLAPIIPQLSEAIYGQLGY-S 484

Query: 560 DQFAAATW------NETKWGGLKAGQVMAPPNPVFARI 590
            +F  +         + +WG L A Q +A P P+F ++
Sbjct: 485 IRFNGSIAPDLLGDRQAQWGVLPASQPLANPEPIFQKL 519

[Close]

BLAST of Carg04512 vs. ExPASy Swiss-Prot
Match: O67298 (Methionine--tRNA ligase OS=Aquifex aeolicus (strain VF5) OX=224324 GN=metG PE=1 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 8.2e-122
Identity = 224/480 (46.67%), Postives = 295/480 (61.46%), Query Frame = 0


Query: 80  FVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIFITGTDEHGEKIATAAAAQG 139
           F +TTP+YYVN  PH+G AYTTIAAD IAR+ RL    V F+TGTDEHG KI   A   G
Sbjct: 7   FYVTTPIYYVNDVPHLGHAYTTIAADTIARYYRLRDYDVFFLTGTDEHGLKIQKKAEELG 66

Query: 140 SGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVKEFYSNVLANGDIYRADYEG 199
             P E  D  +E +K LW+ L I Y KFIRTTDP H   V++ +      GDIY  +YEG
Sbjct: 67  ISPKELVDRNAERFKKLWEFLKIEYTKFIRTTDPYHVKFVQKVFEECYKRGDIYLGEYEG 126

Query: 200 LYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALSKYQRLLEETLTQNPGFVQP 259
            YCV CEE+K E EL  D+ CPIH K C   KE +YFF LSKYQ  L E   +NP F+QP
Sbjct: 127 WYCVGCEEFKSEAELAEDHTCPIHQKKCEYIKEPSYFFRLSKYQDKLLELYEKNPEFIQP 186

Query: 260 AFRLNEVQSWIKSGLKDFSIS--RASVEWGIPVPNDQKQTIYVWFDALLGYISALSENDE 319
            +R NE+ S++K GLKD S++  R+ V+WGIPVP D + TIYVWFDAL  YISAL +   
Sbjct: 187 DYRRNEIISFVKQGLKDLSVTRPRSRVKWGIPVPFDPEHTIYVWFDALFNYISALEDK-- 246

Query: 320 QPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLPKMVFGHGFLTKDGMKMG 379
                  V   WPA LHL+GKDILRFH VYWPA LMS G +LPK VF HG+ T +G KM 
Sbjct: 247 -------VEIYWPADLHLVGKDILRFHTVYWPAFLMSLGYELPKKVFAHGWWTVEGKKMS 306

Query: 380 KSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFINIVNAHLANTIGNLLNR 439
           K+LGN ++P E+VQ++G D VRYF LREV FG DGD+++   +N +N  LAN IGNL +R
Sbjct: 307 KTLGNVVDPYEVVQEYGLDEVRYFLLREVPFGQDGDFSKKAILNRINGELANEIGNLYSR 366

Query: 440 TLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEELSLSSACEAVLEIGNAG 499
            + +  K      +    + A    + +  +E +    N+ E+++   A E +L+  +  
Sbjct: 367 VVNMAHK-----FLGGEVSGARDEEYAKIAQESIKNYENYMEKVNFYKAIEEILKFTSYL 426

Query: 500 NSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSPVTPRLSWRIYEQLGYPE 558
           N Y+DE+ PW+L K+     +   K L  +++ + ++   L P+TP       + LG  E
Sbjct: 427 NKYVDEKQPWALNKE--RKKEELQKVLYALVDGLFVLTHLLYPITPNKMKEALQMLGEKE 470

[Close]

BLAST of Carg04512 vs. ExPASy TrEMBL
Match: A0A6J1H8X3 (Methionine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111461165 PE=3 SV=1)

HSP 1 Score: 1220.7 bits (3157), Expect = 0.0e+00
Identity = 613/618 (99.19%), Postives = 613/618 (99.19%), Query Frame = 0


Query: 1   MAARINTSIQNSLWLLNPLQSSRNLKATHLRIRFNFYQNLLSAPSSCSSSCSSSSSKRAI 60
           MAARINTSIQNSLWLLNPLQSSRNLKATHLR RFNFYQNLLSAP    SSCSSSSSKRAI
Sbjct: 1   MAARINTSIQNSLWLLNPLQSSRNLKATHLRTRFNFYQNLLSAP----SSCSSSSSKRAI 60

Query: 61  FCSSASNFNSHDGGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 120
           FCSSASNFNSHDGGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF
Sbjct: 61  FCSSASNFNSHDGGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 120

Query: 121 ITGTDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVK 180
           ITGTDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVK
Sbjct: 121 ITGTDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVK 180

Query: 181 EFYSNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS 240
           EFYSNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS
Sbjct: 181 EFYSNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS 240

Query: 241 KYQRLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQKQTIYV 300
           KYQRLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQKQTIYV
Sbjct: 241 KYQRLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQKQTIYV 300

Query: 301 WFDALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 360
           WFDALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP
Sbjct: 301 WFDALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 360

Query: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFI 420
           KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFI
Sbjct: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFI 420

Query: 421 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEE 480
           NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEE
Sbjct: 421 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEE 480

Query: 481 LSLSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSP 540
           LSLSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSP
Sbjct: 481 LSLSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSP 540

Query: 541 VTPRLSWRIYEQLGYPEDQFAAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEA 600
           VTPRLSWRIYEQLGYPEDQFAAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEA
Sbjct: 541 VTPRLSWRIYEQLGYPEDQFAAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEA 600

Query: 601 PKKPVKSKKKIAQKVAEA 619
           PKKPVKSKKKIAQKVAEA
Sbjct: 601 PKKPVKSKKKIAQKVAEA 614

[Close]

BLAST of Carg04512 vs. ExPASy TrEMBL
Match: A0A6J1JHL9 (Methionine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111487082 PE=3 SV=1)

HSP 1 Score: 1206.4 bits (3120), Expect = 0.0e+00
Identity = 606/618 (98.06%), Postives = 609/618 (98.54%), Query Frame = 0


Query: 1   MAARINTSIQNSLWLLNPLQSSRNLKATHLRIRFNFYQNLLSAPSSCSSSCSSSSSKRAI 60
           MAARINTSIQNSLWLLNPLQ+SRNLKATHLR RF FYQNLLSAPSSC     SSSSKRAI
Sbjct: 1   MAARINTSIQNSLWLLNPLQNSRNLKATHLRTRFKFYQNLLSAPSSC-----SSSSKRAI 60

Query: 61  FCSSASNFNSHDGGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 120
           FCSSASNFNSHDGGAAT PFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF
Sbjct: 61  FCSSASNFNSHDGGAATGPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 120

Query: 121 ITGTDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVK 180
           ITGTDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVK
Sbjct: 121 ITGTDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVK 180

Query: 181 EFYSNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS 240
           EFYSNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS
Sbjct: 181 EFYSNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS 240

Query: 241 KYQRLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQKQTIYV 300
           KYQRLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQ+QTIYV
Sbjct: 241 KYQRLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQRQTIYV 300

Query: 301 WFDALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 360
           WFDALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP
Sbjct: 301 WFDALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 360

Query: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFI 420
           KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFI
Sbjct: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFI 420

Query: 421 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEE 480
           NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEE
Sbjct: 421 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEE 480

Query: 481 LSLSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSP 540
           LSLSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSP
Sbjct: 481 LSLSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSP 540

Query: 541 VTPRLSWRIYEQLGYPEDQFAAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEA 600
           VTPRLSWRIYEQLG+PEDQFAAATWNETKWGGLKAGQVMA PNPVFARIEQAIVNGEEEA
Sbjct: 541 VTPRLSWRIYEQLGFPEDQFAAATWNETKWGGLKAGQVMALPNPVFARIEQAIVNGEEEA 600

Query: 601 PKKPVKSKKKIAQKVAEA 619
           PKKPVKSKKKIAQKVAEA
Sbjct: 601 PKKPVKSKKKIAQKVAEA 613

[Close]

BLAST of Carg04512 vs. ExPASy TrEMBL
Match: A0A0A0KQV7 (Methionine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_5G512910 PE=3 SV=1)

HSP 1 Score: 1134.0 bits (2932), Expect = 0.0e+00
Identity = 569/618 (92.07%), Postives = 583/618 (94.34%), Query Frame = 0


Query: 1   MAARINTSIQNSLWLLNPLQSSRNLKATHLRIRFNFYQNLLSAPSSCSSSCSSSSSKRAI 60
           MAARINTSIQ SLWLLNP  SS NLK TH R R +F QNLLS P       S SSSKRAI
Sbjct: 1   MAARINTSIQTSLWLLNPFHSSLNLKPTHFRTRLHFCQNLLSFP-------SHSSSKRAI 60

Query: 61  FCSSASNFNSHDGGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 120
           FC+S SNFNSHD GAATDPFVITTPLYYVNAAPHMGSAYTTIAADA+ARFQRLLGKKVIF
Sbjct: 61  FCTSTSNFNSHDSGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAVARFQRLLGKKVIF 120

Query: 121 ITGTDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVK 180
           ITGTDEHGEKIATAAA QGS PSEHCD ISEAYKTLWKDLD+AYDKFIRTTDPKHE IVK
Sbjct: 121 ITGTDEHGEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVK 180

Query: 181 EFYSNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS 240
           EFYS VLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS
Sbjct: 181 EFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS 240

Query: 241 KYQRLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQKQTIYV 300
           KYQRLLEETLTQNPGFVQPAFRLNEVQSWI+SGLKDFSISRASVEWGIPVPND+KQTIYV
Sbjct: 241 KYQRLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDEKQTIYV 300

Query: 301 WFDALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 360
           WFDALLGYISALSEN EQPNLQ  VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP
Sbjct: 301 WFDALLGYISALSENGEQPNLQEVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 360

Query: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFI 420
           KMVFGHGFLTKDGMKMGKSLGNTLEPNELV+KFGSD VRYFFLREVEFGSDGDYAEDRFI
Sbjct: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFI 420

Query: 421 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEE 480
           NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDS+AAA GNAFK+KVEELV KARN+YEE
Sbjct: 421 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSTAAADGNAFKDKVEELVVKARNNYEE 480

Query: 481 LSLSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSP 540
           L+LSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASS+AAAKDLVLILEAMRIIAIALSP
Sbjct: 481 LALSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSP 540

Query: 541 VTPRLSWRIYEQLGYPEDQFAAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEA 600
           VTPRLSWRIY QLGY +DQF AATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEA
Sbjct: 541 VTPRLSWRIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEA 600

Query: 601 PKKPVKSKKKIAQKVAEA 619
           PKKPVKSKKKIAQ VAE+
Sbjct: 601 PKKPVKSKKKIAQAVAES 611

[Close]

BLAST of Carg04512 vs. ExPASy TrEMBL
Match: A0A5D3C1K0 (Methionine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G003330 PE=3 SV=1)

HSP 1 Score: 1121.3 bits (2899), Expect = 0.0e+00
Identity = 565/618 (91.42%), Postives = 579/618 (93.69%), Query Frame = 0


Query: 1   MAARINTSIQNSLWLLNPLQSSRNLKATHLRIRFNFYQNLLSAPSSCSSSCSSSSSKRAI 60
           MAARINTSIQ SL LLNP  +S NLK TH R R +FYQNLL       S  S SSSKRAI
Sbjct: 1   MAARINTSIQTSLSLLNPFHNSLNLKPTHFRTRLHFYQNLL-------SFASYSSSKRAI 60

Query: 61  FCSSASNFNSHDGGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 120
           FC++ SNFNSHD GAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVI 
Sbjct: 61  FCTTTSNFNSHDTGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIL 120

Query: 121 ITGTDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVK 180
           ITGTDEHGEKIATAAA QGS PSEHCD ISEAYKTLWKDLD+AYDKFIRTTDPKHE IVK
Sbjct: 121 ITGTDEHGEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVK 180

Query: 181 EFYSNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS 240
           EFYS VLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS
Sbjct: 181 EFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS 240

Query: 241 KYQRLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQKQTIYV 300
           KYQRLLEETLTQNPGFVQPAFRLNEVQSWI+SGLKDFSISRASVEWGIPVPND+KQTIYV
Sbjct: 241 KYQRLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDKKQTIYV 300

Query: 301 WFDALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 360
           WFDALLGYISALSEN EQPNL   VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP
Sbjct: 301 WFDALLGYISALSENGEQPNLHKVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 360

Query: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFI 420
           KMVFGHGFLTKDGMKMGKSLGNTLEPNELV+KFGSD VRYFFLREVEFGSDGDYAEDRFI
Sbjct: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFI 420

Query: 421 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEE 480
           NIVNAHLANTIGNLLNRTLGLLKKNCQSTL+VDS+AAA GNAFK+KVEELV KARNHYEE
Sbjct: 421 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLLVDSTAAADGNAFKDKVEELVVKARNHYEE 480

Query: 481 LSLSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSP 540
           L+LSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASS+AAAKDLVLILEAMRIIAIALSP
Sbjct: 481 LALSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSP 540

Query: 541 VTPRLSWRIYEQLGYPEDQFAAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEA 600
           VTPRLSWRIY QLGY +DQF AATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEE 
Sbjct: 541 VTPRLSWRIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEV 600

Query: 601 PKKPVKSKKKIAQKVAEA 619
           PKKPVKSKKKIAQ VAEA
Sbjct: 601 PKKPVKSKKKIAQGVAEA 611

[Close]

BLAST of Carg04512 vs. ExPASy TrEMBL
Match: A0A1S3CJS7 (Methionine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103501636 PE=3 SV=1)

HSP 1 Score: 1120.5 bits (2897), Expect = 0.0e+00
Identity = 565/618 (91.42%), Postives = 578/618 (93.53%), Query Frame = 0


Query: 1   MAARINTSIQNSLWLLNPLQSSRNLKATHLRIRFNFYQNLLSAPSSCSSSCSSSSSKRAI 60
           MAARINTSIQ SL LLNP  +S NLK TH R R +FYQNLL       S  S SSSKRAI
Sbjct: 1   MAARINTSIQTSLSLLNPFHNSLNLKPTHFRTRLHFYQNLL-------SFASYSSSKRAI 60

Query: 61  FCSSASNFNSHDGGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 120
           FC++ SNFNSHD GAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVI 
Sbjct: 61  FCTTTSNFNSHDTGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIL 120

Query: 121 ITGTDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVK 180
           ITGTDEHGEKIATAAA QGS PSEHCD ISEAYKTLWKDLD+AYDKFIRTTDPKHE IVK
Sbjct: 121 ITGTDEHGEKIATAAATQGSTPSEHCDIISEAYKTLWKDLDVAYDKFIRTTDPKHEAIVK 180

Query: 181 EFYSNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS 240
           EFYS VLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS
Sbjct: 181 EFYSKVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS 240

Query: 241 KYQRLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQKQTIYV 300
           KYQRLLEETLTQNPGFVQPAFRLNEVQSWI+SGLKDFSISRASVEWGIPVPND KQTIYV
Sbjct: 241 KYQRLLEETLTQNPGFVQPAFRLNEVQSWIRSGLKDFSISRASVEWGIPVPNDXKQTIYV 300

Query: 301 WFDALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 360
           WFDALLGYISALSEN EQPNL   VASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP
Sbjct: 301 WFDALLGYISALSENGEQPNLHKVVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 360

Query: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFI 420
           KMVFGHGFLTKDGMKMGKSLGNTLEPNELV+KFGSD VRYFFLREVEFGSDGDYAEDRFI
Sbjct: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVEKFGSDAVRYFFLREVEFGSDGDYAEDRFI 420

Query: 421 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEE 480
           NIVNAHLANTIGNLLNRTLGLLKKNCQSTL+VDS+AAA GNAFK+KVEELV KARNHYEE
Sbjct: 421 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLLVDSTAAADGNAFKDKVEELVVKARNHYEE 480

Query: 481 LSLSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSP 540
           L+LSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASS+AAAKDLVLILEAMRIIAIALSP
Sbjct: 481 LALSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSNAAAKDLVLILEAMRIIAIALSP 540

Query: 541 VTPRLSWRIYEQLGYPEDQFAAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEA 600
           VTPRLSWRIY QLGY +DQF AATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEE 
Sbjct: 541 VTPRLSWRIYAQLGYSKDQFDAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGEEEV 600

Query: 601 PKKPVKSKKKIAQKVAEA 619
           PKKPVKSKKKIAQ VAEA
Sbjct: 601 PKKPVKSKKKIAQGVAEA 611

[Close]

BLAST of Carg04512 vs. TAIR 10
Match: AT3G55400.1 (methionyl-tRNA synthetase / methionine--tRNA ligase / MetRS (cpMetRS) )

HSP 1 Score: 902.1 bits (2330), Expect = 2.4e-262
Identity = 458/616 (74.35%), Postives = 513/616 (83.28%), Query Frame = 0


Query: 1   MAARINTSIQNSLWLLNPLQSSRNLKATHLRIRFNFYQNLLSAPSSCSSSCSSSSSKRAI 60
           MAARINTS+ N+L  L P  +  N K       F+F +N              SS KRA+
Sbjct: 1   MAARINTSLHNALSFLKPFNTPLNTKP------FSFRRNSFRFSKKLPYYSQFSSGKRAL 60

Query: 61  FCSSASNFNSHDGGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 120
           +C+S+S  ++ D G   + FV+TTPLYYVNA PHMGSAYTTIAAD+IARFQRLLGKKVIF
Sbjct: 61  YCTSSSQESTVDEG---ETFVLTTPLYYVNAPPHMGSAYTTIAADSIARFQRLLGKKVIF 120

Query: 121 ITGTDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVK 180
           ITGTDEHGEKIAT+AAA G  P EHCD IS++Y+TLWKDLDIAYDKFIRTTDPKHE IVK
Sbjct: 121 ITGTDEHGEKIATSAAANGRNPPEHCDLISQSYRTLWKDLDIAYDKFIRTTDPKHEAIVK 180

Query: 181 EFYSNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS 240
           EFY+ V ANGDIYRADYEGLYCVNCEEYKDEKELL +NCCP+H  PC +RKEDNYFFALS
Sbjct: 181 EFYARVFANGDIYRADYEGLYCVNCEEYKDEKELLENNCCPVHQMPCVARKEDNYFFALS 240

Query: 241 KYQRLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQKQTIYV 300
           KYQ+ LE+ L QNP FVQP++RLNEVQSWIKSGL+DFSISRA V+WGIPVP+D KQTIYV
Sbjct: 241 KYQKPLEDILAQNPRFVQPSYRLNEVQSWIKSGLRDFSISRALVDWGIPVPDDDKQTIYV 300

Query: 301 WFDALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 360
           WFDALLGYISAL+E+++Q NL+TAV+ GWPASLHLIGKDILRFHAVYWPAMLMSAGL+LP
Sbjct: 301 WFDALLGYISALTEDNKQQNLETAVSFGWPASLHLIGKDILRFHAVYWPAMLMSAGLELP 360

Query: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFI 420
           KMVFGHGFLTKDGMKMGKSLGNTLEP ELVQKFG D VRYFFLREVEFG+DGDY+EDRFI
Sbjct: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPFELVQKFGPDAVRYFFLREVEFGNDGDYSEDRFI 420

Query: 421 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEE 480
            IVNAHLANTIGNLLNRTLGLLKKNC+STLVVDS+ AA G   K+ VE+LV KAR +YE 
Sbjct: 421 KIVNAHLANTIGNLLNRTLGLLKKNCESTLVVDSTVAAEGVPLKDTVEKLVEKARTNYEN 480

Query: 481 LSLSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSP 540
           LSLSSACEAVLEIGNAGN+YMD+RAPW LFKQGG S++ AAKDLV+ILE MR+IA+ALSP
Sbjct: 481 LSLSSACEAVLEIGNAGNTYMDQRAPWFLFKQGGVSAEEAAKDLVIILEVMRVIAVALSP 540

Query: 541 VTPRLSWRIYEQLGYPEDQFAAATWNETKWGGLKAGQVMAPPNPVFARIEQAIVNGE-EE 600
           V P LS RIY QLGY EDQF + TW++TKWGGLK GQVM   +PVFARIE   +N E EE
Sbjct: 541 VAPCLSLRIYSQLGYTEDQFNSITWSDTKWGGLKGGQVMEQASPVFARIE---LNPEKEE 600

Query: 601 APKKPVKSKKKIAQKV 616
             KKP   KK    KV
Sbjct: 601 DEKKPKVGKKTGKAKV 604

[Close]

BLAST of Carg04512 vs. TAIR 10
Match: AT3G55400.2 (methionyl-tRNA synthetase / methionine--tRNA ligase / MetRS (cpMetRS) )

HSP 1 Score: 801.2 bits (2068), Expect = 5.8e-232
Identity = 399/524 (76.15%), Postives = 446/524 (85.11%), Query Frame = 0


Query: 1   MAARINTSIQNSLWLLNPLQSSRNLKATHLRIRFNFYQNLLSAPSSCSSSCSSSSSKRAI 60
           MAARINTS+ N+L  L P  +  N K       F+F +N              SS KRA+
Sbjct: 1   MAARINTSLHNALSFLKPFNTPLNTKP------FSFRRNSFRFSKKLPYYSQFSSGKRAL 60

Query: 61  FCSSASNFNSHDGGAATDPFVITTPLYYVNAAPHMGSAYTTIAADAIARFQRLLGKKVIF 120
           +C+S+S  ++ D G   + FV+TTPLYYVNA PHMGSAYTTIAAD+IARFQRLLGKKVIF
Sbjct: 61  YCTSSSQESTVDEG---ETFVLTTPLYYVNAPPHMGSAYTTIAADSIARFQRLLGKKVIF 120

Query: 121 ITGTDEHGEKIATAAAAQGSGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVK 180
           ITGTDEHGEKIAT+AAA G  P EHCD IS++Y+TLWKDLDIAYDKFIRTTDPKHE IVK
Sbjct: 121 ITGTDEHGEKIATSAAANGRNPPEHCDLISQSYRTLWKDLDIAYDKFIRTTDPKHEAIVK 180

Query: 181 EFYSNVLANGDIYRADYEGLYCVNCEEYKDEKELLADNCCPIHLKPCASRKEDNYFFALS 240
           EFY+ V ANGDIYRADYEGLYCVNCEEYKDEKELL +NCCP+H  PC +RKEDNYFFALS
Sbjct: 181 EFYARVFANGDIYRADYEGLYCVNCEEYKDEKELLENNCCPVHQMPCVARKEDNYFFALS 240

Query: 241 KYQRLLEETLTQNPGFVQPAFRLNEVQSWIKSGLKDFSISRASVEWGIPVPNDQKQTIYV 300
           KYQ+ LE+ L QNP FVQP++RLNEVQSWIKSGL+DFSISRA V+WGIPVP+D KQTIYV
Sbjct: 241 KYQKPLEDILAQNPRFVQPSYRLNEVQSWIKSGLRDFSISRALVDWGIPVPDDDKQTIYV 300

Query: 301 WFDALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSAGLKLP 360
           WFDALLGYISAL+E+++Q NL+TAV+ GWPASLHLIGKDILRFHAVYWPAMLMSAGL+LP
Sbjct: 301 WFDALLGYISALTEDNKQQNLETAVSFGWPASLHLIGKDILRFHAVYWPAMLMSAGLELP 360

Query: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFGSDPVRYFFLREVEFGSDGDYAEDRFI 420
           KMVFGHGFLTKDGMKMGKSLGNTLEP ELVQKFG D VRYFFLREVEFG+DGDY+EDRFI
Sbjct: 361 KMVFGHGFLTKDGMKMGKSLGNTLEPFELVQKFGPDAVRYFFLREVEFGNDGDYSEDRFI 420

Query: 421 NIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDSSAAAVGNAFKEKVEELVAKARNHYEE 480
            IVNAHLANTIGNLLNRTLGLLKKNC+STLVVDS+ AA G   K+ VE+LV KAR +YE 
Sbjct: 421 KIVNAHLANTIGNLLNRTLGLLKKNCESTLVVDSTVAAEGVPLKDTVEKLVEKARTNYEN 480

Query: 481 LSLSSACEAVLEIGNAGNSYMDERAPWSLFKQGGASSDAAAKDL 525
           LSLSSACEAVLEIGNAGN+YMD+RAPW LFKQGG S++ AAK L
Sbjct: 481 LSLSSACEAVLEIGNAGNTYMDQRAPWFLFKQGGVSAEEAAKVL 515

[Close]

BLAST of Carg04512 vs. TAIR 10
Match: AT4G13780.1 (methionine--tRNA ligase, putative / methionyl-tRNA synthetase, putative / MetRS, putative )

HSP 1 Score: 169.1 bits (427), Expect = 1.1e-41
Identity = 145/570 (25.44%), Postives = 247/570 (43.33%), Query Frame = 0


Query: 81  VITTPLYYVNAAPHMGSAY-TTIAADAIARFQRLLGKKVIFITGTDEHGEKIATAAAAQG 140
           +IT+ L YVN  PH+G+     ++AD  AR+ RL G   I+I GTDE+G    T A  + 
Sbjct: 20  LITSALPYVNNVPHLGNIIGCVLSADVYARYCRLRGYNAIYICGTDEYGTATETKALEEN 79

Query: 141 SGPSEHCDNISEAYKTLWKDLDIAYDKFIRTTDPKHETIVKEFYSNVLANGDIYRADYEG 200
             P E CD     +K ++    I++DKF RT+ P+   + +  ++ +  N  +     + 
Sbjct: 80  CTPKEICDKYHAIHKEVYDWFGISFDKFGRTSTPEQTEVCQAIFNKLWDNKWLSENTMQQ 139

Query: 201 LYCVNCEEYKDEKELLADNCCPIH----------------------------LKPCAS-- 260
           LYC  C+++  ++  L +  CP                               K C +  
Sbjct: 140 LYCDTCKKFLADR--LVEGSCPFEGCNYDSARGDQCEKCGKLLNPTELKDPKCKVCQNTP 199

Query: 261 --RKEDNYFFALSKYQRLLEETLTQNPGFVQPAFRLNEVQ---SWIKSGLKDFSISRASV 320
             R  D+ F  L   +  LE  + +    V  ++  N +Q   +W++ GL+   I+R  +
Sbjct: 200 RIRDTDHLFIELPLLKDRLEAYIKKTS--VTGSWSQNAIQTTNAWLRDGLRQRCITR-DL 259

Query: 321 EWGIPVPND--QKQTIYVWFDALLGYISALSENDEQPNLQTAVASGW---PASLHL---I 380
           +WG+PVP++  + +  YVWFDA +GY+S  S         T+    W   P ++ L   +
Sbjct: 260 KWGVPVPHEKYKDKVFYVWFDAPIGYVSITS-------CYTSEWEKWWKNPENVELYQFM 319

Query: 381 GKDILRFHAVYWPAMLMSA--GLKLPKMVFGHGFLTKDGMKMGKSLGNTLEPNELVQKFG 440
           GKD + FH V +P+  +       L K +    +L  +  K  KS G  +  N++  K  
Sbjct: 320 GKDNVPFHTVMFPSTQLGTEENWTLMKTISVTEYLNYEDGKFSKSKGVGVFGNDV--KDT 379

Query: 441 SDPV---RYFFLREVEFGSDGDYAEDRFINIVNAHLANTIGNLLNRTLGLLKK--NCQST 500
           + PV   RY+ L      SD  ++       +N  L + +GN +NR L  + K  N    
Sbjct: 380 NIPVEVWRYYLLTNRPEVSDTSFSWTDLQAKLNGELLSNLGNFVNRVLSFIAKPDNAGYG 439

Query: 501 LVV----DSSAAAVGNAFKEKVEELVAKARNHYEELSLSSACEAVLEIGNAGNSYMDERA 560
            V+    D+ + ++  +  EKVE+ VA+     E++ L    +  + I + GN Y+    
Sbjct: 440 SVIPDAHDAESHSLTKSLAEKVEKFVAEYVEAMEKVKLKQGLKTAMLISSEGNYYLQASQ 499

Query: 561 PWSLFKQGGASSDAAAKDLVLILEAMRIIAIALSPVTPRLSWRIYEQLGYP-----EDQF 591
            W L+K+        A  +      + ++A  L P  P  S  +++QL  P      D+ 
Sbjct: 500 FWKLYKE---DKPLCAVVIRTAAGLVHLLAQLLEPFMPSFSCEVFKQLNLPPQFSLSDER 559

[Close]

BLAST of Carg04512 vs. TAIR 10
Match: AT5G49030.1 (tRNA synthetase class I (I, L, M and V) family protein )

HSP 1 Score: 49.3 bits (116), Expect = 1.3e-05
Identity = 73/312 (23.40%), Postives = 123/312 (39.42%), Query Frame = 0


Query: 297 TIYVWFDALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSA- 356
           T+ VWFD+   +   L + +            +PA ++L G D    H  ++ + L+++ 
Sbjct: 652 TMDVWFDSGSSWAGVLGKRE---------GLSFPADVYLEGTD---QHRGWFQSSLLTSI 711

Query: 357 ---GLKLPKMVFGHGF-LTKDGMKMGKSLGNTLEPNELVQ---------KFGSDPVRYFF 416
              G      V  HGF L + GMKM KSLGN ++P  +++          +G+D +R + 
Sbjct: 712 ATQGKAPYSAVITHGFVLDEKGMKMSKSLGNVVDPRLVIEGGKNSKDAPAYGADVMRLWV 771

Query: 417 LREVEFGSDGDYAEDRFINIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDS----SAAA 476
                  S  DY  D  I        + I   L  TL  L  N     V ++        
Sbjct: 772 -------SSVDYTGDVLIGPQILRQMSDIYRKLRGTLRYLLGNLHDWRVDNAVPYQDLPI 831

Query: 477 VGNAFKEKVEELVAKARNHYEELSLSSACEAV--LEIGNAGNSYMDERAPWSLFKQGGAS 536
           +      ++E +V   +  YE        + +    I +  N Y D  A   L+  G +S
Sbjct: 832 IDQHALFQLENVVKNIQECYENYQFFKIFQIIQRFTIVDLSNFYFD-IAKDRLYTGGTSS 891

Query: 537 SDAAAKDLVLILEAMRIIAIALSPVTPRLSWRIYEQLGYP---EDQFAAATWNETKWGGL 586
               +   VL    + I+ + ++P+ P L+  +++ L +    ED  AA    E KW  L
Sbjct: 892 FTRRSCQTVLSTHLLSILRV-IAPIVPHLAEDVWQNLPFEYRNEDGSAAEFVFELKWPTL 942

[Close]

BLAST of Carg04512 vs. TAIR 10
Match: AT5G49030.2 (tRNA synthetase class I (I, L, M and V) family protein )

HSP 1 Score: 49.3 bits (116), Expect = 1.3e-05
Identity = 73/312 (23.40%), Postives = 123/312 (39.42%), Query Frame = 0


Query: 297 TIYVWFDALLGYISALSENDEQPNLQTAVASGWPASLHLIGKDILRFHAVYWPAMLMSA- 356
           T+ VWFD+   +   L + +            +PA ++L G D    H  ++ + L+++ 
Sbjct: 652 TMDVWFDSGSSWAGVLGKRE---------GLSFPADVYLEGTD---QHRGWFQSSLLTSI 711

Query: 357 ---GLKLPKMVFGHGF-LTKDGMKMGKSLGNTLEPNELVQ---------KFGSDPVRYFF 416
              G      V  HGF L + GMKM KSLGN ++P  +++          +G+D +R + 
Sbjct: 712 ATQGKAPYSAVITHGFVLDEKGMKMSKSLGNVVDPRLVIEGGKNSKDAPAYGADVMRLWV 771

Query: 417 LREVEFGSDGDYAEDRFINIVNAHLANTIGNLLNRTLGLLKKNCQSTLVVDS----SAAA 476
                  S  DY  D  I        + I   L  TL  L  N     V ++        
Sbjct: 772 -------SSVDYTGDVLIGPQILRQMSDIYRKLRGTLRYLLGNLHDWRVDNAVPYQDLPI 831

Query: 477 VGNAFKEKVEELVAKARNHYEELSLSSACEAV--LEIGNAGNSYMDERAPWSLFKQGGAS 536
           +      ++E +V   +  YE        + +    I +  N Y D  A   L+  G +S
Sbjct: 832 IDQHALFQLENVVKNIQECYENYQFFKIFQIIQRFTIVDLSNFYFD-IAKDRLYTGGTSS 891

Query: 537 SDAAAKDLVLILEAMRIIAIALSPVTPRLSWRIYEQLGYP---EDQFAAATWNETKWGGL 586
               +   VL    + I+ + ++P+ P L+  +++ L +    ED  AA    E KW  L
Sbjct: 892 FTRRSCQTVLSTHLLSILRV-IAPIVPHLAEDVWQNLPFEYRNEDGSAAEFVFELKWPTL 942

The following BLAST results are available for this feature:

BLAST of Carg04512 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7033041.1	0.0e+00	100.00	Methionine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyros...	[more]
XP_022960433.1	0.0e+00	99.19	methionine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita moschata]	[more]
KAG6602362.1	0.0e+00	98.87	Methionine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyros...	[more]
XP_023538063.1	0.0e+00	98.38	methionine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita pepo subsp. pepo...	[more]
XP_022990077.1	0.0e+00	98.06	methionine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita maxima]	[more]

BLAST of Carg04512 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M2T9	3.4e-261	74.35	Methionine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=...	[more]
Q55729	1.1e-161	53.36	Methionine--tRNA ligase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=11117...	[more]
Q8Z068	2.4e-158	52.87	Methionine--tRNA ligase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) ...	[more]
P59081	2.2e-151	50.97	Methionine--tRNA ligase OS=Thermosynechococcus elongatus (strain BP-1) OX=197221...	[more]
O67298	8.2e-122	46.67	Methionine--tRNA ligase OS=Aquifex aeolicus (strain VF5) OX=224324 GN=metG PE=1 ...	[more]

BLAST of Carg04512 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H8X3	0.0e+00	99.19	Methionine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111461165 PE=3 SV=1	[more]
A0A6J1JHL9	0.0e+00	98.06	Methionine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111487082 PE=3 SV=1	[more]
A0A0A0KQV7	0.0e+00	92.07	Methionine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_5G512910 PE=3 SV=1	[more]
A0A5D3C1K0	0.0e+00	91.42	Methionine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A1S3CJS7	0.0e+00	91.42	Methionine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103501636 PE=3 SV=1	[more]

BLAST of Carg04512 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G55400.1	2.4e-262	74.35	methionyl-tRNA synthetase / methionine--tRNA ligase / MetRS (cpMetRS)	[more]
AT3G55400.2	5.8e-232	76.15	methionyl-tRNA synthetase / methionine--tRNA ligase / MetRS (cpMetRS)	[more]
AT4G13780.1	1.1e-41	25.44	methionine--tRNA ligase, putative / methionyl-tRNA synthetase, putative / MetRS,...	[more]
AT5G49030.1	1.3e-05	23.40	tRNA synthetase class I (I, L, M and V) family protein	[more]
AT5G49030.2	1.3e-05	23.40	tRNA synthetase class I (I, L, M and V) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 464..484
None	No IPR available	GENE3D	2.170.220.10		coord: 191..300 e-value: 3.6E-140 score: 468.8
None	No IPR available	GENE3D	1.10.730.10		coord: 415..593 e-value: 6.1E-44 score: 151.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 599..618
None	No IPR available	PANTHER	PTHR43326:SF6	BNAA09G34980D PROTEIN	coord: 1..615
None	No IPR available	PANTHER	PTHR43326	METHIONYL-TRNA SYNTHETASE	coord: 1..615
None	No IPR available	SUPERFAMILY	52374	Nucleotidylyl transferase	coord: 78..424
IPR033911	Methioninyl-tRNA synthetase core domain	PRINTS	PR01041	TRNASYNTHMET	coord: 82..95 score: 56.61 coord: 426..437 score: 51.85 coord: 298..309 score: 72.53 coord: 334..349 score: 65.28 coord: 114..128 score: 54.81 coord: 162..173 score: 57.72
IPR033911	Methioninyl-tRNA synthetase core domain	CDD	cd00814	MetRS_core	coord: 79..414 e-value: 1.07817E-166 score: 476.254
IPR013155	Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase, anticodon-binding	PFAM	PF08264	Anticodon_1	coord: 465..570 e-value: 5.0E-7 score: 29.8
IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	GENE3D	3.40.50.620	HUPs	coord: 80..411 e-value: 3.6E-140 score: 468.8
IPR014758	Methionyl-tRNA synthetase	TIGRFAM	TIGR00398	TIGR00398	coord: 81..589 e-value: 2.9E-155 score: 515.9
IPR015413	Methionyl/Leucyl tRNA synthetase	PFAM	PF09334	tRNA-synt_1g	coord: 214..439 e-value: 2.1E-61 score: 207.8
IPR002300	Aminoacyl-tRNA synthetase, class Ia	PFAM	PF00133	tRNA-synt_1	coord: 77..134 e-value: 1.0E-7 score: 30.6
IPR023457	Methionine-tRNA synthetase, type 2	HAMAP	MF_01228	Met_tRNA_synth_type2	coord: 76..585 score: 42.861732
IPR041872	Methionyl-tRNA synthetase, anticodon-binding domain	CDD	cd07957	Anticodon_Ia_Met	coord: 423..553 e-value: 2.43338E-37 score: 132.999
IPR009080	Aminoacyl-tRNA synthetase, class Ia, anticodon-binding	SUPERFAMILY	47323	Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases	coord: 427..589

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg04512-RA	Carg04512-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006431	methionyl-tRNA aminoacylation
biological_process	GO:0006418	tRNA aminoacylation for protein translation
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004825	methionine-tRNA ligase activity
molecular_function	GO:0004812	aminoacyl-tRNA ligase activity
molecular_function	GO:0000166	nucleotide binding