Carg04503 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg04503
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionCASP-like protein
LocationCarg_Chr04: 20118663 .. 20120973 (+)
RNA-Seq ExpressionCarg04503
SyntenyCarg04503
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGCCTCGAAGGGTTCAAGAATTGCTTCACTGATTCTAAGAATTCTGACCTTCATTTTGATCTTCATTTCTCTCATAATCAACGCTACCAATTCTAAAACTCTCAACAAAGGGACTGAGAATGAAGCAAAGCTTGAATTTAAGAACGTTTATTCATATCGGTAAGTCTTTTTTTATTTTTTATTTTTTCACTAATTCTTGATTTAATGTTCTTGGGGATGTATGTGTTCCAGTTATTTGATAGCAGCGGCTGTAATTGGAGGTGTTTTGAGTTTGATTCAAATCATTCTCAGCATTTACCATATGGTTACCAAAAGCGAAGGAACTCCGCTCTTTTATTTGTTCAGCGATTACGTAAGTGCATGTAAAAAAGTGTTTTTAAGAGCTTGAAAAGTTGTTTTGGATGAACGTTAAACGATGTTATTGTATGTAGTTGTTGTCGTATCTGTTGTTATCGAGCTCGTCGGCGGCGTTAGGAGCTGGGATCGATATGAGAGCTAATTTGAAGGAGCTGTTTGGAACGCTTTTCGACTCGTTTTTCGACCAGGGGAGTGGTGCAGCTGCTGTTCTTTTTATTGCATTCGTTTGCGCTGCTGTTGTCTCAATTCTCTCTGCATTGGCCCTCATCAGGAAGCCTTGAATCAGTGGTGGTTTCTTTGTTTGATAAATTATGATCATTTTTATTTACTTTTTGTATGTGTGTATCCTTTTCCATTTTTAATTTGTAATATGTGATTTAGATTAATGAATATCTAATTTCATCTAATTAATTATGTCTCAATCACCAGATATTCAACATAATTTTGTTCTAATTTACTTCATCAATTAAATTATCAATGGTTTTCAACCATCATACATCATCGATGTTTAATATATTTTTCGAAAGAATATTTCATGTCAATTGGTGAGGTCAACCAGTGCTTGCGAGAATGCTGAGTTTAGAAAAGAGAAACTAACCATTTTTTTTACAAGGGTGTAAAAACCTCTCCTGCCAACAAGGTTTTAAAAATTTTGAGGGAAAGAAAGTTGGAACAAACCATATCTGTAATAGTGGACTTGAATGGTTATTGAAAGAAACTATTTTCAAGAGTTTTTTTTTATGAATTTGAAATGGCGTACTTCTTTTAATAACTAACCTAATCCAAAATATAACTTTGGGGGCAAGATAGTTCGAAAAAATTATATTCAATTATTAAAAAGATGACTAATGCAAATATTCTAAGATATTTAAAGAAAACATCGCCAATTTTATAATGAAAGAATTAAATAATGAATAGAGTAAGACAGATGAAGAGAATTTCACCTTATTTTCCAATTTTATGAACTAAGATCAATGATTAAATATTGAATTCTAATGACATAGTCCATAATTTAAATACTTTATTTAGAAAAAAAATGGATTCTCAATCCATTTGGAAATATTATAGAATATAAGTTATAGATAATTTCCATTATTTATTTATTTATTTATTTATTGAAAATCTTGAGATGAACATAATAAATAAAAAATTGGCCAAGAAAATACCAATTCCAGCTTGTTGACTGTTTCTTTGCTTTTCTTCTACAAAGAAGATATCTCCAGAATGCTTCCATATATATATATATATGTATGTATGTAAGTATATGTGTGTGTATATAGTAAAAGTTGAAGAGCAAAATTTACAAGGGTGGAAGGAGGAGATGGAAGGGTCAAAGGGCTCAAGAATTGCTTCACTGATCCTAAGAATTCTGACCTTCATTTTGATCTTCATTTCTCTCATAATCAACGCCACCAATTCCAAAACTTTCAACAAAGGGGATCAAAATGAAACAACATTTAAGTTTAAAGATGTTTATTCTTATCGGTAAGTGTGTTTTTTCTTTTAGAATTCTCAGCTATTTTTGATTCAATCTGGTTCGGGTTATGTATAGATATTTGATATCAACTACGGTTATTGGAGCTGTTTTGAGTCTACTTCAAATCGCTCTCAACATTTACAACGTCGTTACCAAAAGCCATCGAACTCTACTCTTCGATATGTTCAGCGACAAGGTAAGTATACTAATATTTGTTTGAGCTTTCGAGATTTAAGGATGTTTAATGTCATGTTATGTTATGTAGCTGTTGTCGTATCTTCTACTGTCGGGGGCCTCGGCGGGGTTAGGTGCTGGCATCGATTTGAGAGTCGGTCTTAAAGAGCTGGTTGGCAACCTTTTAAACTCGTTTTTCGACAAGGGAAATGGTTCGGCGGCTGTTCTTCTCCTTGCGTTTATTTGCGCTGCCGTTGTCTCAATTCTCTCTTCCTTGGCCCTCATCAGGAAGCCTTGA

mRNA sequence

ATGGCAGCCTCGAAGGGTTCAAGAATTGCTTCACTGATTCTAAGAATTCTGACCTTCATTTTGATCTTCATTTCTCTCATAATCAACGCTACCAATTCTAAAACTCTCAACAAAGGGACTGAGAATGAAGCAAAGCTTGAATTTAAGAACGTTTATTCATATCGTTATTTGATAGCAGCGGCTGTAATTGGAGGTGTTTTGAGTTTGATTCAAATCATTCTCAGCATTTACCATATGGTTACCAAAAGCGAAGGAACTCCGCTCTTTTATTTGTTCAGCGATTACTTGTTGTCGTATCTGTTGTTATCGAGCTCGTCGGCGGCGTTAGGAGCTGGGATCGATATGAGAGCTAATTTGAAGGAGCTGTTTGGAACGCTTTTCGACTCGTTTTTCGACCAGGGGAGTGGTGCAGCTGCTGTTCTTTTTATTGCATTCGTTTGCGCTGCTGTTGGCTCAAGAATTGCTTCACTGATCCTAAGAATTCTGACCTTCATTTTGATCTTCATTTCTCTCATAATCAACGCCACCAATTCCAAAACTTTCAACAAAGGGGATCAAAATGAAACAACATTTAAGTTTAAAGATGTTTATTCTTATCGATATTTGATATCAACTACGGTTATTGGAGCTGTTTTGAGTCTACTTCAAATCGCTCTCAACATTTACAACGTCGTTACCAAAAGCCATCGAACTCTACTCTTCGATATGTTCAGCGACAAGCTGTTGTCGTATCTTCTACTGTCGGGGGCCTCGGCGGGGTTAGGTGCTGGCATCGATTTGAGAGTCGGTCTTAAAGAGCTGGTTGGCAACCTTTTAAACTCGTTTTTCGACAAGGGAAATGGTTCGGCGGCTGTTCTTCTCCTTGCGTTTATTTGCGCTGCCGTTGTCTCAATTCTCTCTTCCTTGGCCCTCATCAGGAAGCCTTGA

Coding sequence (CDS)

ATGGCAGCCTCGAAGGGTTCAAGAATTGCTTCACTGATTCTAAGAATTCTGACCTTCATTTTGATCTTCATTTCTCTCATAATCAACGCTACCAATTCTAAAACTCTCAACAAAGGGACTGAGAATGAAGCAAAGCTTGAATTTAAGAACGTTTATTCATATCGTTATTTGATAGCAGCGGCTGTAATTGGAGGTGTTTTGAGTTTGATTCAAATCATTCTCAGCATTTACCATATGGTTACCAAAAGCGAAGGAACTCCGCTCTTTTATTTGTTCAGCGATTACTTGTTGTCGTATCTGTTGTTATCGAGCTCGTCGGCGGCGTTAGGAGCTGGGATCGATATGAGAGCTAATTTGAAGGAGCTGTTTGGAACGCTTTTCGACTCGTTTTTCGACCAGGGGAGTGGTGCAGCTGCTGTTCTTTTTATTGCATTCGTTTGCGCTGCTGTTGGCTCAAGAATTGCTTCACTGATCCTAAGAATTCTGACCTTCATTTTGATCTTCATTTCTCTCATAATCAACGCCACCAATTCCAAAACTTTCAACAAAGGGGATCAAAATGAAACAACATTTAAGTTTAAAGATGTTTATTCTTATCGATATTTGATATCAACTACGGTTATTGGAGCTGTTTTGAGTCTACTTCAAATCGCTCTCAACATTTACAACGTCGTTACCAAAAGCCATCGAACTCTACTCTTCGATATGTTCAGCGACAAGCTGTTGTCGTATCTTCTACTGTCGGGGGCCTCGGCGGGGTTAGGTGCTGGCATCGATTTGAGAGTCGGTCTTAAAGAGCTGGTTGGCAACCTTTTAAACTCGTTTTTCGACAAGGGAAATGGTTCGGCGGCTGTTCTTCTCCTTGCGTTTATTTGCGCTGCCGTTGTCTCAATTCTCTCTTCCTTGGCCCTCATCAGGAAGCCTTGA

Protein sequence

MAASKGSRIASLILRILTFILIFISLIINATNSKTLNKGTENEAKLEFKNVYSYRYLIAAAVIGGVLSLIQIILSIYHMVTKSEGTPLFYLFSDYLLSYLLLSSSSAALGAGIDMRANLKELFGTLFDSFFDQGSGAAAVLFIAFVCAAVGSRIASLILRILTFILIFISLIINATNSKTFNKGDQNETTFKFKDVYSYRYLISTTVIGAVLSLLQIALNIYNVVTKSHRTLLFDMFSDKLLSYLLLSGASAGLGAGIDLRVGLKELVGNLLNSFFDKGNGSAAVLLLAFICAAVVSILSSLALIRKP
Homology
BLAST of Carg04503 vs. NCBI nr
Match: KAG7033050.1 (CASP-like protein 4D1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 552.0 bits (1421), Expect = 3.3e-153
Identity = 308/308 (100.00%), Postives = 308/308 (100.00%), Query Frame = 0

Query: 1   MAASKGSRIASLILRILTFILIFISLIINATNSKTLNKGTENEAKLEFKNVYSYRYLIAA 60
           MAASKGSRIASLILRILTFILIFISLIINATNSKTLNKGTENEAKLEFKNVYSYRYLIAA
Sbjct: 1   MAASKGSRIASLILRILTFILIFISLIINATNSKTLNKGTENEAKLEFKNVYSYRYLIAA 60

Query: 61  AVIGGVLSLIQIILSIYHMVTKSEGTPLFYLFSDYLLSYLLLSSSSAALGAGIDMRANLK 120
           AVIGGVLSLIQIILSIYHMVTKSEGTPLFYLFSDYLLSYLLLSSSSAALGAGIDMRANLK
Sbjct: 61  AVIGGVLSLIQIILSIYHMVTKSEGTPLFYLFSDYLLSYLLLSSSSAALGAGIDMRANLK 120

Query: 121 ELFGTLFDSFFDQGSGAAAVLFIAFVCAAVGSRIASLILRILTFILIFISLIINATNSKT 180
           ELFGTLFDSFFDQGSGAAAVLFIAFVCAAVGSRIASLILRILTFILIFISLIINATNSKT
Sbjct: 121 ELFGTLFDSFFDQGSGAAAVLFIAFVCAAVGSRIASLILRILTFILIFISLIINATNSKT 180

Query: 181 FNKGDQNETTFKFKDVYSYRYLISTTVIGAVLSLLQIALNIYNVVTKSHRTLLFDMFSDK 240
           FNKGDQNETTFKFKDVYSYRYLISTTVIGAVLSLLQIALNIYNVVTKSHRTLLFDMFSDK
Sbjct: 181 FNKGDQNETTFKFKDVYSYRYLISTTVIGAVLSLLQIALNIYNVVTKSHRTLLFDMFSDK 240

Query: 241 LLSYLLLSGASAGLGAGIDLRVGLKELVGNLLNSFFDKGNGSAAVLLLAFICAAVVSILS 300
           LLSYLLLSGASAGLGAGIDLRVGLKELVGNLLNSFFDKGNGSAAVLLLAFICAAVVSILS
Sbjct: 241 LLSYLLLSGASAGLGAGIDLRVGLKELVGNLLNSFFDKGNGSAAVLLLAFICAAVVSILS 300

Query: 301 SLALIRKP 309
           SLALIRKP
Sbjct: 301 SLALIRKP 308

BLAST of Carg04503 vs. NCBI nr
Match: KAG6602372.1 (CASP-like protein 4D1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 282.3 bits (721), Expect = 4.9e-72
Identity = 156/158 (98.73%), Postives = 158/158 (100.00%), Query Frame = 0

Query: 151 GSRIASLILRILTFILIFISLIINATNSKTFNKGDQNETTFKFKDVYSYRYLISTTVIGA 210
           GSRIASLILRILTFILIFISLIINATNSKTFNKGDQNETTFKFKDVYSYRYLISTT+IGA
Sbjct: 6   GSRIASLILRILTFILIFISLIINATNSKTFNKGDQNETTFKFKDVYSYRYLISTTIIGA 65

Query: 211 VLSLLQIALNIYNVVTKSHRTLLFDMFSDKLLSYLLLSGASAGLGAGIDLRVGLKELVGN 270
           VLSLLQIALNIYNVVTKSHRTLLF+MFSDKLLSYLLLSGASAGLGAGIDLRVGLKELVGN
Sbjct: 66  VLSLLQIALNIYNVVTKSHRTLLFNMFSDKLLSYLLLSGASAGLGAGIDLRVGLKELVGN 125

Query: 271 LLNSFFDKGNGSAAVLLLAFICAAVVSILSSLALIRKP 309
           LLNSFFDKGNGSAAVLLLAFICAAVVSILSSLALIRKP
Sbjct: 126 LLNSFFDKGNGSAAVLLLAFICAAVVSILSSLALIRKP 163

BLAST of Carg04503 vs. NCBI nr
Match: XP_022954145.1 (CASP-like protein 4D1 [Cucurbita moschata])

HSP 1 Score: 278.1 bits (710), Expect = 9.2e-71
Identity = 155/158 (98.10%), Postives = 156/158 (98.73%), Query Frame = 0

Query: 151 GSRIASLILRILTFILIFISLIINATNSKTFNKGDQNETTFKFKDVYSYRYLISTTVIGA 210
           GSRIASLILRILTFILIFISLIINATNSKTFNKG +NETTFKFKDVYSYRYLISTTVIGA
Sbjct: 6   GSRIASLILRILTFILIFISLIINATNSKTFNKGAENETTFKFKDVYSYRYLISTTVIGA 65

Query: 211 VLSLLQIALNIYNVVTKSHRTLLFDMFSDKLLSYLLLSGASAGLGAGIDLRVGLKELVGN 270
           VLSLLQIALNIYNVVTKSHRTLLFDMFSDKLLSYLLLSGASAGLGAGIDLRVGLKELVGN
Sbjct: 66  VLSLLQIALNIYNVVTKSHRTLLFDMFSDKLLSYLLLSGASAGLGAGIDLRVGLKELVGN 125

Query: 271 LLNSFFDKGNGSAAVLLLAFICAAVVSILSSLALIRKP 309
           LLNSFFDKGN SAAVLLLAFICAAVVSILSSLALIRKP
Sbjct: 126 LLNSFFDKGNASAAVLLLAFICAAVVSILSSLALIRKP 163

BLAST of Carg04503 vs. NCBI nr
Match: XP_023525049.1 (CASP-like protein 4D1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 274.2 bits (700), Expect = 1.3e-69
Identity = 153/158 (96.84%), Postives = 154/158 (97.47%), Query Frame = 0

Query: 151 GSRIASLILRILTFILIFISLIINATNSKTFNKGDQNETTFKFKDVYSYRYLISTTVIGA 210
           GSRIASLILRILTFILIFISLIINATNSKTFNKG QNET FKFKDVYSYRYLIST +IGA
Sbjct: 6   GSRIASLILRILTFILIFISLIINATNSKTFNKGAQNETKFKFKDVYSYRYLISTMIIGA 65

Query: 211 VLSLLQIALNIYNVVTKSHRTLLFDMFSDKLLSYLLLSGASAGLGAGIDLRVGLKELVGN 270
           VLSLLQIALNIYNVVTKSHRTLLFDMFSDKLLSYLLLSGASAGLGAGIDLRVGLKELVGN
Sbjct: 66  VLSLLQIALNIYNVVTKSHRTLLFDMFSDKLLSYLLLSGASAGLGAGIDLRVGLKELVGN 125

Query: 271 LLNSFFDKGNGSAAVLLLAFICAAVVSILSSLALIRKP 309
           LLNSFFDKGN SAAVLLLAFICAAVVSILSSLALIRKP
Sbjct: 126 LLNSFFDKGNASAAVLLLAFICAAVVSILSSLALIRKP 163

BLAST of Carg04503 vs. NCBI nr
Match: XP_022963262.1 (CASP-like protein 4D1 [Cucurbita moschata] >KAG6602371.1 CASP-like protein 4D1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 273.9 bits (699), Expect = 1.7e-69
Identity = 153/159 (96.23%), Postives = 156/159 (98.11%), Query Frame = 0

Query: 1   MAASKGSRIASLILRILTFILIFISLIINATNSKTLNKGTENEAKLEFKNVYSYRYLIAA 60
           MAASKGSRIASLILRILTFILIFISLIINATNSKTLNKGTENEAKLEFKNVYSYRYLIAA
Sbjct: 1   MAASKGSRIASLILRILTFILIFISLIINATNSKTLNKGTENEAKLEFKNVYSYRYLIAA 60

Query: 61  AVIGGVLSLIQIILSIYHMVTKSEGTPLFYLFSDYLLSYLLLSSSSAALGAGIDMRANLK 120
           AVIGGVLSLIQIILSIYHMVTKSEGTPLFYLFSDYLLSYLLLSSSSAALGAGIDMRANLK
Sbjct: 61  AVIGGVLSLIQIILSIYHMVTKSEGTPLFYLFSDYLLSYLLLSSSSAALGAGIDMRANLK 120

Query: 121 ELFGTLFDSFFDQGSGAAAVLFIAFVCAAVGSRIASLIL 160
           ELFGTLFDSFFDQGSGAAAVLFIAFVCAAV S +++L L
Sbjct: 121 ELFGTLFDSFFDQGSGAAAVLFIAFVCAAVVSILSALAL 159

BLAST of Carg04503 vs. ExPASy Swiss-Prot
Match: C6T1Z6 (CASP-like protein 4D1 OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 1.8e-24
Identity = 73/166 (43.98%), Postives = 107/166 (64.46%), Query Frame = 0

Query: 148 AAVGSRIASLILRILTFILIFISLIINATNSKTFNKGDQNETTFKFKDVYSYRYLISTTV 207
           ++ GSR   L+LR+LTF+ + I+LI+ A   +T    D+     KF D+Y+YRY+IST +
Sbjct: 13  SSTGSRTVLLLLRVLTFVFLLIALILIAIVKQT---DDETGVEIKFNDIYAYRYMISTII 72

Query: 208 IGAVLSLLQIALNIYNVVTKSHRTL------LFDMFSDKLLSYLLLSGASAGLGAGIDLR 267
           IG   +LLQ+AL+I+ VV+  +R L      LFD F DK++SYLL+SG++AG G  ++L 
Sbjct: 73  IGFAYNLLQMALSIFTVVS-GNRVLSGDGGYLFDFFGDKIISYLLISGSAAGFGVTVELG 132

Query: 268 VGLKELVGNLLNSFFDKGNGSAAVLLLAFICAAVVSILSSLALIRK 308
            G+        NSF DK N SA++LL+AF+  AV S  +S AL +K
Sbjct: 133 RGVPS------NSFMDKANASASLLLIAFLFTAVASTFTSFALPKK 168

BLAST of Carg04503 vs. ExPASy Swiss-Prot
Match: B9SXY8 (CASP-like protein 4D1 OS=Ricinus communis OX=3988 GN=RCOM_1206790 PE=3 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 1.7e-22
Identity = 69/161 (42.86%), Postives = 102/161 (63.35%), Query Frame = 0

Query: 149 AVGSRIASLILRILTFILIFISLIINATNSKTFNKGDQNETTFKFKDVYSYRYLISTTVI 208
           ++ SR+A+LILRILTFI +  SL+I  TN+ T    D  E    FKDVY+YRY+++T VI
Sbjct: 7   SLASRMAALILRILTFIFLIASLVILTTNTATLEL-DLVEVKVHFKDVYAYRYMLATIVI 66

Query: 209 GAVLSLLQIALNIYNVVTKSHR-----TLLFDMFSDKLLSYLLLSGASAGLGAGIDLRVG 268
           G   ++LQIA  +Y V T +        L FD F DK++SY+L++GA+AG  +  D++  
Sbjct: 67  GLAYTVLQIAFTLYYVATGNRMMSGDGNLAFDFFGDKVISYILVTGAAAGFASTKDIKPV 126

Query: 269 LKELVGNLLNSFFDKGNGSAAVLLLAFICAAVVSILSSLAL 305
                    ++F +KG  SA++LL+ F+C AV+S+ SS AL
Sbjct: 127 FSG--SGDFDAFINKGYASASLLLIGFVCTAVLSVFSSYAL 164

BLAST of Carg04503 vs. ExPASy Swiss-Prot
Match: B9NBE5 (CASP-like protein 4D1 OS=Populus trichocarpa OX=3694 GN=POPTR_0010s21230g PE=2 SV=2)

HSP 1 Score: 103.2 bits (256), Expect = 5.3e-21
Identity = 65/162 (40.12%), Postives = 100/162 (61.73%), Query Frame = 0

Query: 152 SRIASLILRILTFILIFISLIINATNSKTFNKGDQNETTFKFKDVYSYRYLISTTVIGAV 211
           SR+ +L LR+LTF  + +SL+I  TN+ T   G  +E   + KD YSYRY+++    G  
Sbjct: 19  SRMTALFLRVLTFAFLMVSLVIMTTNTGTIEIG-IDEFKVRSKDFYSYRYMLAAIAFGLT 78

Query: 212 LSLLQIALNIYNV------VTKSHRTLLFDMFSDKLLSYLLLSGASAGLGAGIDLRVGLK 271
            ++LQIAL + ++       T     L+FD + DK++SY+L +GA+A  GA  +L+  L 
Sbjct: 79  YTILQIALTLNHISKRNGAQTSGDGNLVFDFYGDKVVSYILATGAAAAFGATKELKTQLA 138

Query: 272 ELVGNLLNSFFDKGNGSAAVLLLAFICAAVVSILSSLALIRK 308
            L G   + FF+KG  SA++LLL F+C A++S+ SS AL +K
Sbjct: 139 GLGG---DKFFNKGYASASLLLLGFVCTAILSVFSSYALPKK 176

BLAST of Carg04503 vs. ExPASy Swiss-Prot
Match: Q56X75 (CASP-like protein 4D2 OS=Arabidopsis thaliana OX=3702 GN=At2g39518 PE=2 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 1.0e-19
Identity = 66/165 (40.00%), Postives = 100/165 (60.61%), Query Frame = 0

Query: 149 AVGSRIASLILRILTFILIFISLIINATNSKTFNKGDQNETTFKFKDVYSYRYLISTTVI 208
           AV  ++  L+LR+LT + + I+LII +TNS T      +   F FKDVY+YRY++S  VI
Sbjct: 10  AVSLKVLLLLLRVLTGVFLVIALIILSTNSVTI-VSQGSALKFHFKDVYAYRYMLSAAVI 69

Query: 209 GAVLSLLQIALNIYNVVT--KSHRTLLFDMFSDKLLSYLLLSGASAGLGAGIDLRVGLKE 268
           G V +++Q+   I    T  K+      D + DKL+SYL+ +G++AG G   DL+     
Sbjct: 70  GLVYAVIQLFFTISEFATGVKNPFNYQLDFYGDKLISYLVATGSAAGFGVTKDLKDTFLA 129

Query: 269 LVG----NLLNSFFDKGNGSAAVLLLAFICAAVVSILSSLALIRK 308
           LV     + ++ FF KG  SA++LL AFIC AV+S+ SS A+ ++
Sbjct: 130 LVALDSTDPVDKFFSKGYASASLLLFAFICLAVLSVFSSFAMAKR 173

BLAST of Carg04503 vs. ExPASy Swiss-Prot
Match: D7LD59 (CASP-like protein 4D2 OS=Arabidopsis lyrata subsp. lyrata OX=81972 GN=ARALYDRAFT_903204 PE=3 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 2.9e-19
Identity = 63/164 (38.41%), Postives = 101/164 (61.59%), Query Frame = 0

Query: 150 VGSRIASLILRILTFILIFISLIINATNSKTFNKGDQNETTFKFKDVYSYRYLISTTVIG 209
           V  +++ L+LR+LT + + I+LII +TNS T      +   F FKDVY+YRY++S  VIG
Sbjct: 11  VSLKVSLLLLRVLTGVFLVIALIILSTNSVTI-VSQGSALKFHFKDVYAYRYMLSAAVIG 70

Query: 210 AVLSLLQIALNIYNVVT--KSHRTLLFDMFSDKLLSYLLLSGASAGLGAGIDLRVGLKEL 269
            + +++Q+   I    T  K+      D + DKL+SYL+ +G++AG G   DL+     L
Sbjct: 71  LLYAVIQLFFTISEFATGMKNPFNYQLDFYGDKLISYLVATGSAAGFGVSKDLKDAFIAL 130

Query: 270 VG----NLLNSFFDKGNGSAAVLLLAFICAAVVSILSSLALIRK 308
           V     + ++ FF +G  SA++LL +FIC AV+S+ SSLA+ ++
Sbjct: 131 VALDSTDPVDKFFSRGYASASLLLFSFICLAVLSVFSSLAIAKR 173

BLAST of Carg04503 vs. ExPASy TrEMBL
Match: A0A6J1GRM4 (CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111456492 PE=3 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 4.5e-71
Identity = 155/158 (98.10%), Postives = 156/158 (98.73%), Query Frame = 0

Query: 151 GSRIASLILRILTFILIFISLIINATNSKTFNKGDQNETTFKFKDVYSYRYLISTTVIGA 210
           GSRIASLILRILTFILIFISLIINATNSKTFNKG +NETTFKFKDVYSYRYLISTTVIGA
Sbjct: 6   GSRIASLILRILTFILIFISLIINATNSKTFNKGAENETTFKFKDVYSYRYLISTTVIGA 65

Query: 211 VLSLLQIALNIYNVVTKSHRTLLFDMFSDKLLSYLLLSGASAGLGAGIDLRVGLKELVGN 270
           VLSLLQIALNIYNVVTKSHRTLLFDMFSDKLLSYLLLSGASAGLGAGIDLRVGLKELVGN
Sbjct: 66  VLSLLQIALNIYNVVTKSHRTLLFDMFSDKLLSYLLLSGASAGLGAGIDLRVGLKELVGN 125

Query: 271 LLNSFFDKGNGSAAVLLLAFICAAVVSILSSLALIRKP 309
           LLNSFFDKGN SAAVLLLAFICAAVVSILSSLALIRKP
Sbjct: 126 LLNSFFDKGNASAAVLLLAFICAAVVSILSSLALIRKP 163

BLAST of Carg04503 vs. ExPASy TrEMBL
Match: A0A6J1HET3 (CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111463525 PE=3 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 8.4e-70
Identity = 153/159 (96.23%), Postives = 156/159 (98.11%), Query Frame = 0

Query: 1   MAASKGSRIASLILRILTFILIFISLIINATNSKTLNKGTENEAKLEFKNVYSYRYLIAA 60
           MAASKGSRIASLILRILTFILIFISLIINATNSKTLNKGTENEAKLEFKNVYSYRYLIAA
Sbjct: 1   MAASKGSRIASLILRILTFILIFISLIINATNSKTLNKGTENEAKLEFKNVYSYRYLIAA 60

Query: 61  AVIGGVLSLIQIILSIYHMVTKSEGTPLFYLFSDYLLSYLLLSSSSAALGAGIDMRANLK 120
           AVIGGVLSLIQIILSIYHMVTKSEGTPLFYLFSDYLLSYLLLSSSSAALGAGIDMRANLK
Sbjct: 61  AVIGGVLSLIQIILSIYHMVTKSEGTPLFYLFSDYLLSYLLLSSSSAALGAGIDMRANLK 120

Query: 121 ELFGTLFDSFFDQGSGAAAVLFIAFVCAAVGSRIASLIL 160
           ELFGTLFDSFFDQGSGAAAVLFIAFVCAAV S +++L L
Sbjct: 121 ELFGTLFDSFFDQGSGAAAVLFIAFVCAAVVSILSALAL 159

BLAST of Carg04503 vs. ExPASy TrEMBL
Match: A0A6J1JJD2 (CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111487466 PE=3 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 1.2e-68
Identity = 150/158 (94.94%), Postives = 153/158 (96.84%), Query Frame = 0

Query: 151 GSRIASLILRILTFILIFISLIINATNSKTFNKGDQNETTFKFKDVYSYRYLISTTVIGA 210
           GSRIASLILRILTFILIFISL+INATNSKT NKG +NET FKFKDVYSYRYLISTT+IGA
Sbjct: 6   GSRIASLILRILTFILIFISLLINATNSKTLNKGAENETKFKFKDVYSYRYLISTTIIGA 65

Query: 211 VLSLLQIALNIYNVVTKSHRTLLFDMFSDKLLSYLLLSGASAGLGAGIDLRVGLKELVGN 270
           VLSLLQIALNIYNVVTKSHRTLLFDMFSDKLLSYLLLSGASAGLGAGIDLRVGLKELVGN
Sbjct: 66  VLSLLQIALNIYNVVTKSHRTLLFDMFSDKLLSYLLLSGASAGLGAGIDLRVGLKELVGN 125

Query: 271 LLNSFFDKGNGSAAVLLLAFICAAVVSILSSLALIRKP 309
           LLNSF DKGN SAAVLLLAFICAAVVSILSSLALIRKP
Sbjct: 126 LLNSFLDKGNASAAVLLLAFICAAVVSILSSLALIRKP 163

BLAST of Carg04503 vs. ExPASy TrEMBL
Match: A0A6J1JSF8 (CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111487423 PE=3 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 8.7e-67
Identity = 147/159 (92.45%), Postives = 153/159 (96.23%), Query Frame = 0

Query: 1   MAASKGSRIASLILRILTFILIFISLIINATNSKTLNKGTENEAKLEFKNVYSYRYLIAA 60
           MAASKGSRIA+LILRILT ILIFISLIINA+NSKTLNKGTENEAKLEFKNVYSYRYLIAA
Sbjct: 1   MAASKGSRIATLILRILTLILIFISLIINASNSKTLNKGTENEAKLEFKNVYSYRYLIAA 60

Query: 61  AVIGGVLSLIQIILSIYHMVTKSEGTPLFYLFSDYLLSYLLLSSSSAALGAGIDMRANLK 120
           AVIGGVLSLIQIILSIYHMVTKSEGTPLFYLFSDYLLSYLLLSSSSAALGAGID RANLK
Sbjct: 61  AVIGGVLSLIQIILSIYHMVTKSEGTPLFYLFSDYLLSYLLLSSSSAALGAGIDTRANLK 120

Query: 121 ELFGTLFDSFFDQGSGAAAVLFIAFVCAAVGSRIASLIL 160
           ELFGTLFDSFFDQGSGAAAVL IAF+CAAV S +++L L
Sbjct: 121 ELFGTLFDSFFDQGSGAAAVLLIAFICAAVVSILSALAL 159

BLAST of Carg04503 vs. ExPASy TrEMBL
Match: A0A6J1C1A4 (CASP-like protein OS=Momordica charantia OX=3673 GN=LOC111006513 PE=3 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 7.1e-53
Identity = 123/163 (75.46%), Postives = 139/163 (85.28%), Query Frame = 0

Query: 146 VCAAVGSRIASLILRILTFILIFISLIINATNSKTFNKGDQNETTFKFKDVYSYRYLIST 205
           + A+ GSRIASLILRILTF+LIFISL+I ATNSKT  KG + E   +FKDVYSYRYL + 
Sbjct: 1   MAASKGSRIASLILRILTFVLIFISLLIIATNSKTVLKGTEAEAKVEFKDVYSYRYLTAA 60

Query: 206 TVIGAVLSLLQIALNIYNVVTKSHRTLLFDMFSDKLLSYLLLSGASAGLGAGIDLRVGLK 265
            VIGA LSLLQIAL +Y+VVTKS  T  FDMFSDKLL+YLLLSGASAGLGAGIDLR   K
Sbjct: 61  AVIGAALSLLQIALTLYHVVTKSDGTPFFDMFSDKLLTYLLLSGASAGLGAGIDLRSNYK 120

Query: 266 ELVGNLLNSFFDKGNGSAAVLLLAFICAAVVSILSSLALIRKP 309
           +LVGN+ NSFFDKG+ SAA+LLLAFIC+AVVSILSSLAL+RKP
Sbjct: 121 DLVGNIFNSFFDKGSASAAILLLAFICSAVVSILSSLALVRKP 163

BLAST of Carg04503 vs. TAIR 10
Match: AT2G39518.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 99.0 bits (245), Expect = 7.1e-21
Identity = 66/165 (40.00%), Postives = 100/165 (60.61%), Query Frame = 0

Query: 149 AVGSRIASLILRILTFILIFISLIINATNSKTFNKGDQNETTFKFKDVYSYRYLISTTVI 208
           AV  ++  L+LR+LT + + I+LII +TNS T      +   F FKDVY+YRY++S  VI
Sbjct: 10  AVSLKVLLLLLRVLTGVFLVIALIILSTNSVTI-VSQGSALKFHFKDVYAYRYMLSAAVI 69

Query: 209 GAVLSLLQIALNIYNVVT--KSHRTLLFDMFSDKLLSYLLLSGASAGLGAGIDLRVGLKE 268
           G V +++Q+   I    T  K+      D + DKL+SYL+ +G++AG G   DL+     
Sbjct: 70  GLVYAVIQLFFTISEFATGVKNPFNYQLDFYGDKLISYLVATGSAAGFGVTKDLKDTFLA 129

Query: 269 LVG----NLLNSFFDKGNGSAAVLLLAFICAAVVSILSSLALIRK 308
           LV     + ++ FF KG  SA++LL AFIC AV+S+ SS A+ ++
Sbjct: 130 LVALDSTDPVDKFFSKGYASASLLLFAFICLAVLSVFSSFAMAKR 173

BLAST of Carg04503 vs. TAIR 10
Match: AT2G39530.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 94.4 bits (233), Expect = 1.8e-19
Identity = 66/168 (39.29%), Postives = 105/168 (62.50%), Query Frame = 0

Query: 149 AVGSRIASLILRILTFILIFISLIINATNSKTFNKGDQNETTFK--FKDVYSYRYLISTT 208
           +V  R   L+LR+LT   + I++++ +TN+ T    + + T+ K  F DVY+YRY++S  
Sbjct: 10  SVTLRTVLLLLRVLTAAFLLITVVLISTNTVTL---EISSTSIKLPFNDVYAYRYMLSAA 69

Query: 209 VIGAVLSLLQIALNIYNVVT-KSH-RTLLFDMFSDKLLSYLLLSGASAGLGAGIDLRVGL 268
           VIG V +++Q+ L I    T K+H  T  FD + DK++SYLL +G++AG G   DL+   
Sbjct: 70  VIGLVYAVVQLFLTISQFATGKTHPLTYQFDFYGDKVISYLLATGSAAGFGVSKDLKDTY 129

Query: 269 KELV----GNLLNSFFDKGNGSAAVLLLAFICAAVVSILSSLALIRKP 309
             L+     + ++ FF KG  SA++LL AF+  AV+S+ SSLAL ++P
Sbjct: 130 IALIEFDSTDPVDKFFSKGYASASLLLFAFVSLAVLSVFSSLALSKRP 174

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7033050.13.3e-153100.00CASP-like protein 4D1, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6602372.14.9e-7298.73CASP-like protein 4D1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022954145.19.2e-7198.10CASP-like protein 4D1 [Cucurbita moschata][more]
XP_023525049.11.3e-6996.84CASP-like protein 4D1 [Cucurbita pepo subsp. pepo][more]
XP_022963262.11.7e-6996.23CASP-like protein 4D1 [Cucurbita moschata] >KAG6602371.1 CASP-like protein 4D1, ... [more]
Match NameE-valueIdentityDescription
C6T1Z61.8e-2443.98CASP-like protein 4D1 OS=Glycine max OX=3847 PE=2 SV=1[more]
B9SXY81.7e-2242.86CASP-like protein 4D1 OS=Ricinus communis OX=3988 GN=RCOM_1206790 PE=3 SV=1[more]
B9NBE55.3e-2140.12CASP-like protein 4D1 OS=Populus trichocarpa OX=3694 GN=POPTR_0010s21230g PE=2 S... [more]
Q56X751.0e-1940.00CASP-like protein 4D2 OS=Arabidopsis thaliana OX=3702 GN=At2g39518 PE=2 SV=1[more]
D7LD592.9e-1938.41CASP-like protein 4D2 OS=Arabidopsis lyrata subsp. lyrata OX=81972 GN=ARALYDRAFT... [more]
Match NameE-valueIdentityDescription
A0A6J1GRM44.5e-7198.10CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111456492 PE=3 SV=1[more]
A0A6J1HET38.4e-7096.23CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111463525 PE=3 SV=1[more]
A0A6J1JJD21.2e-6894.94CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111487466 PE=3 SV=1[more]
A0A6J1JSF88.7e-6792.45CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111487423 PE=3 SV=1[more]
A0A6J1C1A47.1e-5375.46CASP-like protein OS=Momordica charantia OX=3673 GN=LOC111006513 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G39518.17.1e-2140.00Uncharacterised protein family (UPF0497) [more]
AT2G39530.11.8e-1939.29Uncharacterised protein family (UPF0497) [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006702Casparian strip membrane protein domainPFAMPF04535DUF588coord: 6..147
e-value: 1.9E-24
score: 86.2
coord: 152..292
e-value: 3.6E-23
score: 82.0
NoneNo IPR availablePANTHERPTHR11615:SF148CASP-LIKE PROTEIN 4D2coord: 152..307
coord: 5..156
NoneNo IPR availablePANTHERPTHR11615NITRATE, FORMATE, IRON DEHYDROGENASEcoord: 152..307
coord: 5..156

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg04503-RACarg04503-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding