Carg04452 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg04452
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionsirohydrochlorin ferrochelatase, chloroplastic
LocationCarg_Chr04: 20353326 .. 20354373 (+)
RNA-Seq ExpressionCarg04452
SyntenyCarg04452
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGATCGTTATCCATCACTCCTCAGATCACAGTGAAGAGGTCTGCAAAATCTTCCCTCTAATTTTCCCTTTTACAATTTGCTTAATCTGCCTCTAATGGCCGTTCGTTTCGCCATTGAAGTTTCTCGGCAGGCGACGCTAGAAGAAGAACATACACAATCAGCACATCTCAGAAATCTGCCATTCCTATCGGACGGCCCTCGAGATTCGGAAATTTCACCTCGCGATTCCGCATGAGTGTCGATAATGGAGGCTCGAGTCAGAGCCGAGATCGGATTGGTGCGGGAGATGCAGTCATTATCGTCGACCATGGCTCCCGGCGGCAGGAATCTAACCTCATGCTGAGTAAGTTAGGGTTTTGGAATTTGGGTGAGATTGCAAATTCTACGCAATCTAATTCTGTTGTGAATTTCAGATGCGTTCGTTGCTATGTTTAGGGATAAAACTGGTTACCCTATTGTGGAGCCTGCTCCCATGGTATGTTCTTCTTAACTGCATTTTTCTTGTGGGGCTGTAATGAATTCTCTTTTACTTTGCTCTAAAAACCACAGGAGCTGGCTAAACCTTCGATAAGAGACGCATTCAAATCGTGCGTTGAACAAGGGGCCAAACGCATCATCATAAGCCCGTTCTTCCTCTTCCCCGGTCGACATTGGCAACAGGTAACTTGCTGTAAGGCAGTTGATTTGATGAAATGACTCTTTCAGTAAGTGGATTGATAGCAATGGCTGGAATTTTGTAGGATATTCCTTCCTTAACAGCTGAGGCAGCAAAGGACCACCCAGGAATATCATACATAGTAACTGCTCCTCTTGGTCTTCATGAAAAGCTTGTGGTAGGAATTTCTCAAGTATAGTGAAGAAACAGGTTCATAAATGATCAAAGAAAAAGAAAAGACTGTATTTGTTAGTATTGACATGTGAGGTGTTCTGTTTGCACTGGCAGGATGTTGTGAATGATAGAATTAATCACTGCTTGAGTCATGCTGCTGGAGTTGCAGATGAATGTGAAGTTTGTGCTGGAACAGGCAAATGCAGATTTAATTAA

mRNA sequence

ATGAGATCGTTATCCATCACTCCTCAGATCACAGTGAAGAGTTTCTCGGCAGGCGACGCTAGAAGAAGAACATACACAATCAGCACATCTCAGAAATCTGCCATTCCTATCGGACGGCCCTCGAGATTCGGAAATTTCACCTCGCGATTCCGCATGAGTGTCGATAATGGAGGCTCGAGTCAGAGCCGAGATCGGATTGGTGCGGGAGATGCAGTCATTATCGTCGACCATGGCTCCCGGCGGCAGGAATCTAACCTCATGCTGAATGCGTTCGTTGCTATGTTTAGGGATAAAACTGGTTACCCTATTGTGGAGCCTGCTCCCATGGAGCTGGCTAAACCTTCGATAAGAGACGCATTCAAATCGTGCGTTGAACAAGGGGCCAAACGCATCATCATAAGCCCGTTCTTCCTCTTCCCCGGTCGACATTGGCAACAGGATATTCCTTCCTTAACAGCTGAGGCAGCAAAGGACCACCCAGGAATATCATACATAGTAACTGCTCCTCTTGGTCTTCATGAAAAGCTTGTGGATGTTGTGAATGATAGAATTAATCACTGCTTGAGTCATGCTGCTGGAGTTGCAGATGAATGTGAAGTTTGTGCTGGAACAGGCAAATGCAGATTTAATTAA

Coding sequence (CDS)

ATGAGATCGTTATCCATCACTCCTCAGATCACAGTGAAGAGTTTCTCGGCAGGCGACGCTAGAAGAAGAACATACACAATCAGCACATCTCAGAAATCTGCCATTCCTATCGGACGGCCCTCGAGATTCGGAAATTTCACCTCGCGATTCCGCATGAGTGTCGATAATGGAGGCTCGAGTCAGAGCCGAGATCGGATTGGTGCGGGAGATGCAGTCATTATCGTCGACCATGGCTCCCGGCGGCAGGAATCTAACCTCATGCTGAATGCGTTCGTTGCTATGTTTAGGGATAAAACTGGTTACCCTATTGTGGAGCCTGCTCCCATGGAGCTGGCTAAACCTTCGATAAGAGACGCATTCAAATCGTGCGTTGAACAAGGGGCCAAACGCATCATCATAAGCCCGTTCTTCCTCTTCCCCGGTCGACATTGGCAACAGGATATTCCTTCCTTAACAGCTGAGGCAGCAAAGGACCACCCAGGAATATCATACATAGTAACTGCTCCTCTTGGTCTTCATGAAAAGCTTGTGGATGTTGTGAATGATAGAATTAATCACTGCTTGAGTCATGCTGCTGGAGTTGCAGATGAATGTGAAGTTTGTGCTGGAACAGGCAAATGCAGATTTAATTAA

Protein sequence

MRSLSITPQITVKSFSAGDARRRTYTISTSQKSAIPIGRPSRFGNFTSRFRMSVDNGGSSQSRDRIGAGDAVIIVDHGSRRQESNLMLNAFVAMFRDKTGYPIVEPAPMELAKPSIRDAFKSCVEQGAKRIIISPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVVNDRINHCLSHAAGVADECEVCAGTGKCRFN
Homology
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BLAST of Carg04452 vs. NCBI nr
Match: KAG7033101.1 (Sirohydrochlorin ferrochelatase, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 425.2 bits (1092), Expect = 3.2e-115
Identity = 210/210 (100.00%), Postives = 210/210 (100.00%), Query Frame = 0 

Query: 1   MRSLSITPQITVKSFSAGDARRRTYTISTSQKSAIPIGRPSRFGNFTSRFRMSVDNGGSS 60
           MRSLSITPQITVKSFSAGDARRRTYTISTSQKSAIPIGRPSRFGNFTSRFRMSVDNGGSS
Sbjct: 1   MRSLSITPQITVKSFSAGDARRRTYTISTSQKSAIPIGRPSRFGNFTSRFRMSVDNGGSS 60

Query: 61  QSRDRIGAGDAVIIVDHGSRRQESNLMLNAFVAMFRDKTGYPIVEPAPMELAKPSIRDAF 120
           QSRDRIGAGDAVIIVDHGSRRQESNLMLNAFVAMFRDKTGYPIVEPAPMELAKPSIRDAF
Sbjct: 61  QSRDRIGAGDAVIIVDHGSRRQESNLMLNAFVAMFRDKTGYPIVEPAPMELAKPSIRDAF 120

Query: 121 KSCVEQGAKRIIISPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVV 180
           KSCVEQGAKRIIISPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVV
Sbjct: 121 KSCVEQGAKRIIISPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVV 180

Query: 181 NDRINHCLSHAAGVADECEVCAGTGKCRFN 211
           NDRINHCLSHAAGVADECEVCAGTGKCRFN
Sbjct: 181 NDRINHCLSHAAGVADECEVCAGTGKCRFN 210

[Close]
BLAST of Carg04452 vs. NCBI nr
Match: XP_023548507.1 (sirohydrochlorin ferrochelatase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 401.7 bits (1031), Expect = 3.8e-108
Identity = 199/210 (94.76%), Postives = 202/210 (96.19%), Query Frame = 0 

Query: 1   MRSLSITPQITVKSFSAGDARRRTYTISTSQKSAIPIGRPSRFGNFTSRFRMSVDNGGSS 60
           MRSLSITPQITVKSFS+GD RRRTYTISTSQKSAIPIGR  RFGN TSRFRMSVDNGGSS
Sbjct: 1   MRSLSITPQITVKSFSSGDTRRRTYTISTSQKSAIPIGRHLRFGNSTSRFRMSVDNGGSS 60

Query: 61  QSRDRIGAGDAVIIVDHGSRRQESNLMLNAFVAMFRDKTGYPIVEPAPMELAKPSIRDAF 120
           +SRDRIGAGDAVIIVDHGSRRQESNLMLN FVAMFRD TGYPIVEPA MELAKPSIRDAF
Sbjct: 61  RSRDRIGAGDAVIIVDHGSRRQESNLMLNEFVAMFRDNTGYPIVEPAHMELAKPSIRDAF 120

Query: 121 KSCVEQGAKRIIISPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVV 180
           KSCVEQGA RIIISPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVV
Sbjct: 121 KSCVEQGANRIIISPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVV 180

Query: 181 NDRINHCLSHAAGVADECEVCAGTGKCRFN 211
           NDR+NHCLSHAAGVADECEVCAGTGKCRFN
Sbjct: 181 NDRMNHCLSHAAGVADECEVCAGTGKCRFN 210

[Close]
BLAST of Carg04452 vs. NCBI nr
Match: XP_038890885.1 (sirohydrochlorin ferrochelatase, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 352.8 bits (904), Expect = 2.0e-93
Identity = 179/211 (84.83%), Postives = 192/211 (91.00%), Query Frame = 0 

Query: 1   MRSLSI-TPQITVKSFSAGDARRRTYTISTSQKSAIPIGRPSRFGNFTSRFRMSVDNGGS 60
           M SLSI TPQI++KS SA + RR+ YTI  SQKS I I R SRF NFTSRFRMSVDNG S
Sbjct: 37  MTSLSIVTPQISLKSSSAVETRRK-YTICASQKSPILIRRLSRFRNFTSRFRMSVDNGSS 96

Query: 61  SQSRDRIGAGDAVIIVDHGSRRQESNLMLNAFVAMFRDKTGYPIVEPAPMELAKPSIRDA 120
           ++S DRIGAGDAVIIVDHGSRR+ESNLMLN FVAMFRDKTGYPIVEPA MELA+PSIRD+
Sbjct: 97  NRSGDRIGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDS 156

Query: 121 FKSCVEQGAKRIIISPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDV 180
           F  CVEQGAKRII+SPFFLFPGRHWQQDIPSLTAEAAKDHPGISYI+TAPLGLHEKLVDV
Sbjct: 157 FALCVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDV 216

Query: 181 VNDRINHCLSHAAGVADECEVCAGTGKCRFN 211
           VNDRI+HCLSHAAGVA+ECEVCAGTGKCRFN
Sbjct: 217 VNDRISHCLSHAAGVAEECEVCAGTGKCRFN 246

[Close]
BLAST of Carg04452 vs. NCBI nr
Match: XP_022134499.1 (sirohydrochlorin ferrochelatase, chloroplastic [Momordica charantia])

HSP 1 Score: 352.1 bits (902), Expect = 3.4e-93
Identity = 175/209 (83.73%), Postives = 187/209 (89.47%), Query Frame = 0 

Query: 1   MRSLSITPQITVKSFSAGDARRRTYTISTSQKSAIPIGRPSRFGNFTSRFRMSVDNGGSS 60
           M SLS  PQITV+  ++G    R Y I  SQKS++PIGR SRF NF SRFRMSVDNGG++
Sbjct: 1   MGSLSFRPQITVERGASGSEAGR-YKICASQKSSVPIGRSSRFRNFASRFRMSVDNGGAN 60

Query: 61  QSRDRIGAGDAVIIVDHGSRRQESNLMLNAFVAMFRDKTGYPIVEPAPMELAKPSIRDAF 120
           +S  RIG+GDAVIIVDHGSRRQESNLMLN FVAMFRDKTGYPIVEPA MELA+PSIRD+F
Sbjct: 61  RSGARIGSGDAVIIVDHGSRRQESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSF 120

Query: 121 KSCVEQGAKRIIISPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVV 180
            SCVEQGAK II+SPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVV
Sbjct: 121 ASCVEQGAKHIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVV 180

Query: 181 NDRINHCLSHAAGVADECEVCAGTGKCRF 210
           NDRINHCLSHAAGVADECEVCAGTGKCRF
Sbjct: 181 NDRINHCLSHAAGVADECEVCAGTGKCRF 208

[Close]
BLAST of Carg04452 vs. NCBI nr
Match: KAA0037556.1 (sirohydrochlorin ferrochelatase [Cucumis melo var. makuwa] >TYK03116.1 sirohydrochlorin ferrochelatase [Cucumis melo var. makuwa])

HSP 1 Score: 345.5 bits (885), Expect = 3.2e-91
Identity = 174/210 (82.86%), Postives = 186/210 (88.57%), Query Frame = 0 

Query: 1   MRSLSITPQITVKSFSAGDARRRTYTISTSQKSAIPIGRPSRFGNFTSRFRMSVDNGGSS 60
           M SLS+TPQI+ KS S  +  R  YTI   Q+S IPIGR S+F NFTSR RMS+DNG S 
Sbjct: 1   MSSLSVTPQISPKSPSPVET-RSPYTICAFQRSPIPIGRLSKFRNFTSRVRMSIDNGSS- 60

Query: 61  QSRDRIGAGDAVIIVDHGSRRQESNLMLNAFVAMFRDKTGYPIVEPAPMELAKPSIRDAF 120
              +RIGAGDAVIIVDHGSRR+ESNLMLN FVAMFRDKTGYPIVEPA MELA+PSIRD+F
Sbjct: 61  ---NRIGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSF 120

Query: 121 KSCVEQGAKRIIISPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVV 180
            SCVEQGAKRII+SPFFLFPGRHWQQDIPSLTAEAAKDHPGISYI+TAPLGLHEKLVDVV
Sbjct: 121 TSCVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVV 180

Query: 181 NDRINHCLSHAAGVADECEVCAGTGKCRFN 211
           NDRINHCLSHAAGVADECEVCAGTGKCRFN
Sbjct: 181 NDRINHCLSHAAGVADECEVCAGTGKCRFN 205

[Close]
BLAST of Carg04452 vs. ExPASy Swiss-Prot
Match: Q84JH7 (Sirohydrochlorin ferrochelatase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SIRB PE=1 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 1.5e-59
Identity = 113/183 (61.75%), Postives = 138/183 (75.41%), Query Frame = 0 

Query: 29  TSQKSAIPIG-RPSRFGNFTSRFRMSVDNGGSSQS--RDRIGAGDAVIIVDHGSRRQESN 88
           T++  A+P+  +  +F     R R      G S+   ++ IG  D +IIVDHGSRR+ESN
Sbjct: 38  TNRSWALPVSFKVEKFQLQRGRRRRGSPCFGESEGLVKNGIGDADGIIIVDHGSRRRESN 97

Query: 89  LMLNAFVAMFRDKTGYPIVEPAPMELAKPSIRDAFKSCVEQGAKRIIISPFFLFPGRHWQ 148
           LML  FV MF++KTGYPIVEPA MELA+PSI+DAF  CV+QGAKR+++SPFFLFPGRHW 
Sbjct: 98  LMLEEFVKMFKEKTGYPIVEPAHMELAEPSIKDAFSLCVQQGAKRVVVSPFFLFPGRHWH 157

Query: 149 QDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVVNDRINHCLSHAAGVADECEVCAGTG 208
            DIPSLTA+AAK+  GISY++TAPLG H  L+DVVNDRI HCLSH  G ADEC VCAGT 
Sbjct: 158 TDIPSLTADAAKEFSGISYLITAPLGPHNLLLDVVNDRIQHCLSHVEGDADECLVCAGTN 217

[Close]
BLAST of Carg04452 vs. ExPASy Swiss-Prot
Match: P61817 (Sirohydrochlorin ferrochelatase OS=Bacillus megaterium OX=1404 GN=sirB PE=3 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 1.2e-16
Identity = 43/116 (37.07%), Postives = 64/116 (55.17%), Query Frame = 0 

Query: 70  DAVIIVDHGSRRQESNLMLNAFVAMFRDKTGYPIVEPAPMELAKPSIRDAFKSCVEQGAK 129
           DAV+ V HGSR +E      AF+   +     PI E   +ELA P+I   F++C+EQGA 
Sbjct: 13  DAVLYVCHGSRVKEGADQAVAFIERCKKNLDVPIQEVCFLELASPTIEQGFEACIEQGAT 72

Query: 130 RIIISPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVVNDRIN 186
           RI I P  L    H + DIP    +  + +P +  +   P G+ E++VD++ +RIN
Sbjct: 73  RIAIVPLLLLTAAHAKHDIPEEIQKVYERYPQVEVLYGEPFGVDERIVDILVERIN 128

[Close]
BLAST of Carg04452 vs. ExPASy Swiss-Prot
Match: Q58380 (Sirohydrochlorin cobaltochelatase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=cbiX PE=1 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 2.8e-13
Identity = 43/138 (31.16%), Postives = 66/138 (47.83%), Query Frame = 0 

Query: 70  DAVIIVDHGSRRQESNLMLNAFVAMFRDKTGYPIVEPAPMELAKPSIRDAFKSCVEQGAK 129
           +A+++V HGSR   S  +L       +++  +PIVE   ME ++P+I  A K  +EQGAK
Sbjct: 2   EALVLVGHGSRLPYSKELLVKLAEKVKERNLFPIVEIGLMEFSEPTIPQAVKKAIEQGAK 61

Query: 130 RIIISPFFLFPGRHWQQDIPSLTAEAAKDH------------------------PGISYI 184
           RII+ P FL  G H  +DIP L      +H                          +  I
Sbjct: 62  RIIVVPVFLAHGIHTTRDIPRLLGLIEDNHEHHHEHSHHHHHHHHHEHEKLEIPEDVEII 121

[Close]
BLAST of Carg04452 vs. ExPASy Swiss-Prot
Match: P61819 (Sirohydrochlorin cobaltochelatase OS=Methanococcus maripaludis (strain S2 / LL) OX=267377 GN=cbiX PE=3 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 6.2e-13
Identity = 39/141 (27.66%), Postives = 66/141 (46.81%), Query Frame = 0 

Query: 70  DAVIIVDHGSRRQESNLMLNAFVAMFRDKTGYPIVEPAPMELAKPSIRDAFKSCVEQGAK 129
           +A+++V HGSR   S  ++       + +  Y IVE   ME  +P+I    K  ++ GAK
Sbjct: 2   EALVLVGHGSRLPHSKNVVMEVAEKIKARNIYDIVEVGMMEFNEPTIPQTIKKVIDAGAK 61

Query: 130 RIIISPFFLFPGRHWQQDIPSLTA------EAAKDH-------------------PGISY 186
           ++I++P FL PG H ++DIP +        E    H                    G+  
Sbjct: 62  KVIVTPVFLAPGNHTERDIPKILGIYEGDDEGGHHHHHDHEHHHHHHDTTAVEIPEGVEL 121

[Close]
BLAST of Carg04452 vs. ExPASy Swiss-Prot
Match: A6UWT5 (Sirohydrochlorin cobaltochelatase OS=Methanococcus aeolicus (strain ATCC BAA-1280 / DSM 17508 / OCM 812 / Nankai-3) OX=419665 GN=cbiX PE=3 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 4.4e-11
Identity = 40/138 (28.99%), Postives = 64/138 (46.38%), Query Frame = 0 

Query: 70  DAVIIVDHGSRRQESNLMLNAFVAMFRDKTGYPIVEPAPMELAKPSIRDAFKSCVEQGAK 129
           +A++++ HGSR   S  ++       ++K  Y IVE   ME  +P+I       + +GAK
Sbjct: 2   EALVLLGHGSRLPYSKEIVGKVAEKIKEKNIYDIVEIGMMEFNEPTIPQTINKVIAEGAK 61

Query: 130 RIIISPFFLFPGRHWQQDIPSLTA--EAAKDH----------------------PGISYI 184
           +III P FL  G H ++DIP +    E  + H                       G+  I
Sbjct: 62  KIIIVPVFLAHGNHTKRDIPQILGLIECEEHHHEGEGGHHHHHHHHHGEKIEVPEGVEII 121

[Close]
BLAST of Carg04452 vs. ExPASy TrEMBL
Match: A0A6J1BYW9 (sirohydrochlorin ferrochelatase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111006726 PE=4 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 1.7e-93
Identity = 175/209 (83.73%), Postives = 187/209 (89.47%), Query Frame = 0 

Query: 1   MRSLSITPQITVKSFSAGDARRRTYTISTSQKSAIPIGRPSRFGNFTSRFRMSVDNGGSS 60
           M SLS  PQITV+  ++G    R Y I  SQKS++PIGR SRF NF SRFRMSVDNGG++
Sbjct: 1   MGSLSFRPQITVERGASGSEAGR-YKICASQKSSVPIGRSSRFRNFASRFRMSVDNGGAN 60

Query: 61  QSRDRIGAGDAVIIVDHGSRRQESNLMLNAFVAMFRDKTGYPIVEPAPMELAKPSIRDAF 120
           +S  RIG+GDAVIIVDHGSRRQESNLMLN FVAMFRDKTGYPIVEPA MELA+PSIRD+F
Sbjct: 61  RSGARIGSGDAVIIVDHGSRRQESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSF 120

Query: 121 KSCVEQGAKRIIISPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVV 180
            SCVEQGAK II+SPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVV
Sbjct: 121 ASCVEQGAKHIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVV 180

Query: 181 NDRINHCLSHAAGVADECEVCAGTGKCRF 210
           NDRINHCLSHAAGVADECEVCAGTGKCRF
Sbjct: 181 NDRINHCLSHAAGVADECEVCAGTGKCRF 208

[Close]
BLAST of Carg04452 vs. ExPASy TrEMBL
Match: A0A5D3BY55 (Sirohydrochlorin ferrochelatase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold374G00520 PE=4 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 1.5e-91
Identity = 174/210 (82.86%), Postives = 186/210 (88.57%), Query Frame = 0 

Query: 1   MRSLSITPQITVKSFSAGDARRRTYTISTSQKSAIPIGRPSRFGNFTSRFRMSVDNGGSS 60
           M SLS+TPQI+ KS S  +  R  YTI   Q+S IPIGR S+F NFTSR RMS+DNG S 
Sbjct: 1   MSSLSVTPQISPKSPSPVET-RSPYTICAFQRSPIPIGRLSKFRNFTSRVRMSIDNGSS- 60

Query: 61  QSRDRIGAGDAVIIVDHGSRRQESNLMLNAFVAMFRDKTGYPIVEPAPMELAKPSIRDAF 120
              +RIGAGDAVIIVDHGSRR+ESNLMLN FVAMFRDKTGYPIVEPA MELA+PSIRD+F
Sbjct: 61  ---NRIGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSF 120

Query: 121 KSCVEQGAKRIIISPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVV 180
            SCVEQGAKRII+SPFFLFPGRHWQQDIPSLTAEAAKDHPGISYI+TAPLGLHEKLVDVV
Sbjct: 121 TSCVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVV 180

Query: 181 NDRINHCLSHAAGVADECEVCAGTGKCRFN 211
           NDRINHCLSHAAGVADECEVCAGTGKCRFN
Sbjct: 181 NDRINHCLSHAAGVADECEVCAGTGKCRFN 205

[Close]
BLAST of Carg04452 vs. ExPASy TrEMBL
Match: A0A1S3C8S9 (sirohydrochlorin ferrochelatase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103498093 PE=4 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 1.5e-91
Identity = 174/210 (82.86%), Postives = 186/210 (88.57%), Query Frame = 0 

Query: 1   MRSLSITPQITVKSFSAGDARRRTYTISTSQKSAIPIGRPSRFGNFTSRFRMSVDNGGSS 60
           M SLS+TPQI+ KS S  +  R  YTI  SQ+S IPIGR S+F NFTSR RMS+DNG S 
Sbjct: 1   MSSLSVTPQISPKSPSPVET-RSPYTICASQRSPIPIGRLSKFRNFTSRVRMSIDNGSS- 60

Query: 61  QSRDRIGAGDAVIIVDHGSRRQESNLMLNAFVAMFRDKTGYPIVEPAPMELAKPSIRDAF 120
              +RIGAGDAVIIVDHGSRR+ESN MLN FVAMFRDKTGYPIVEPA MELA+PSIRD+F
Sbjct: 61  ---NRIGAGDAVIIVDHGSRRRESNRMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSF 120

Query: 121 KSCVEQGAKRIIISPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVV 180
            SCVEQGAKRII+SPFFLFPGRHWQQDIPSLTAEAAKDHPGISYI+TAPLGLHEKLVDVV
Sbjct: 121 TSCVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVV 180

Query: 181 NDRINHCLSHAAGVADECEVCAGTGKCRFN 211
           NDRINHCLSHAAGVADECEVCAGTGKCRFN
Sbjct: 181 NDRINHCLSHAAGVADECEVCAGTGKCRFN 205

[Close]
BLAST of Carg04452 vs. ExPASy TrEMBL
Match: A0A6J1K384 (sirohydrochlorin ferrochelatase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111489668 PE=4 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 2.0e-91
Identity = 175/212 (82.55%), Postives = 190/212 (89.62%), Query Frame = 0 

Query: 1   MRSLSITPQITVKSFSAGDARRRTYTISTSQKSA--IPIGRPSRFGNFTSRFRMSVDNGG 60
           M SLSI PQITVKS SAG+  R+TYTI  SQKS+  I IGR S   NF+SRFRMSVDNGG
Sbjct: 1   MTSLSIPPQITVKSPSAGET-RKTYTICASQKSSILIGIGRSSTLRNFSSRFRMSVDNGG 60

Query: 61  SSQSRDRIGAGDAVIIVDHGSRRQESNLMLNAFVAMFRDKTGYPIVEPAPMELAKPSIRD 120
           S    +RIGAGDAVIIVDHGSRR+ESNLMLN FVAMFRDKTGYPIVEPA MELA+PSIRD
Sbjct: 61  S----NRIGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRD 120

Query: 121 AFKSCVEQGAKRIIISPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVD 180
           +F SCVEQGAKRI++SPFFLFPGRHWQQDIPSLTAEAAKDHPGISYI+TAPLGLHE+LVD
Sbjct: 121 SFASCVEQGAKRIVVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVD 180

Query: 181 VVNDRINHCLSHAAGVADECEVCAGTGKCRFN 211
           VVNDRI+HCLSHAAGV +ECEVCAGTGKC+FN
Sbjct: 181 VVNDRISHCLSHAAGVVEECEVCAGTGKCKFN 207

[Close]
BLAST of Carg04452 vs. ExPASy TrEMBL
Match: A0A6J1FHT5 (sirohydrochlorin ferrochelatase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111445470 PE=4 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 1.3e-90
Identity = 173/212 (81.60%), Postives = 189/212 (89.15%), Query Frame = 0 

Query: 1   MRSLSITPQITVKSFSAGDARRRTYTISTSQKSA--IPIGRPSRFGNFTSRFRMSVDNGG 60
           M SLSI  QITVKS SAG+  R+TYTI  SQKS+  I +GR S   NF+SRFRMSVDNGG
Sbjct: 1   MTSLSIPSQITVKSPSAGET-RKTYTICASQKSSILIGVGRSSTLRNFSSRFRMSVDNGG 60

Query: 61  SSQSRDRIGAGDAVIIVDHGSRRQESNLMLNAFVAMFRDKTGYPIVEPAPMELAKPSIRD 120
           +    DRIGAGDAVIIVDHGSRR+ESNLMLN FVAMFRDKTGYPIVEPA MELA+PSIRD
Sbjct: 61  A----DRIGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRD 120

Query: 121 AFKSCVEQGAKRIIISPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVD 180
           +F SCVEQGAKRI++SPFFLFPGRHWQQDIPSLTAEAAKDHPGISYI+TAPLGLHE+LVD
Sbjct: 121 SFASCVEQGAKRIVVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVD 180

Query: 181 VVNDRINHCLSHAAGVADECEVCAGTGKCRFN 211
           VVNDRI+HCLSHAAGV +ECEVCAGTGKC+FN
Sbjct: 181 VVNDRISHCLSHAAGVVEECEVCAGTGKCKFN 207

[Close]
BLAST of Carg04452 vs. TAIR 10
Match: AT1G50170.1 (sirohydrochlorin ferrochelatase B )

HSP 1 Score: 230.7 bits (587), Expect = 1.1e-60
Identity = 113/183 (61.75%), Postives = 138/183 (75.41%), Query Frame = 0 

Query: 29  TSQKSAIPIG-RPSRFGNFTSRFRMSVDNGGSSQS--RDRIGAGDAVIIVDHGSRRQESN 88
           T++  A+P+  +  +F     R R      G S+   ++ IG  D +IIVDHGSRR+ESN
Sbjct: 38  TNRSWALPVSFKVEKFQLQRGRRRRGSPCFGESEGLVKNGIGDADGIIIVDHGSRRRESN 97

Query: 89  LMLNAFVAMFRDKTGYPIVEPAPMELAKPSIRDAFKSCVEQGAKRIIISPFFLFPGRHWQ 148
           LML  FV MF++KTGYPIVEPA MELA+PSI+DAF  CV+QGAKR+++SPFFLFPGRHW 
Sbjct: 98  LMLEEFVKMFKEKTGYPIVEPAHMELAEPSIKDAFSLCVQQGAKRVVVSPFFLFPGRHWH 157

Query: 149 QDIPSLTAEAAKDHPGISYIVTAPLGLHEKLVDVVNDRINHCLSHAAGVADECEVCAGTG 208
            DIPSLTA+AAK+  GISY++TAPLG H  L+DVVNDRI HCLSH  G ADEC VCAGT 
Sbjct: 158 TDIPSLTADAAKEFSGISYLITAPLGPHNLLLDVVNDRIQHCLSHVEGDADECLVCAGTN 217

The following BLAST results are available for this feature:
BLAST of Carg04452 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG7033101.13.2e-115100.00Sirohydrochlorin ferrochelatase, chloroplastic, partial [Cucurbita argyrosperma ... [more]
XP_023548507.13.8e-10894.76sirohydrochlorin ferrochelatase, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_038890885.12.0e-9384.83sirohydrochlorin ferrochelatase, chloroplastic isoform X1 [Benincasa hispida][more]
XP_022134499.13.4e-9383.73sirohydrochlorin ferrochelatase, chloroplastic [Momordica charantia][more]
KAA0037556.13.2e-9182.86sirohydrochlorin ferrochelatase [Cucumis melo var. makuwa] >TYK03116.1 sirohydro... [more]
BLAST of Carg04452 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q84JH71.5e-5961.75Sirohydrochlorin ferrochelatase, chloroplastic OS=Arabidopsis thaliana OX=3702 G... [more]
P618171.2e-1637.07Sirohydrochlorin ferrochelatase OS=Bacillus megaterium OX=1404 GN=sirB PE=3 SV=1[more]
Q583802.8e-1331.16Sirohydrochlorin cobaltochelatase OS=Methanocaldococcus jannaschii (strain ATCC ... [more]
P618196.2e-1327.66Sirohydrochlorin cobaltochelatase OS=Methanococcus maripaludis (strain S2 / LL) ... [more]
A6UWT54.4e-1128.99Sirohydrochlorin cobaltochelatase OS=Methanococcus aeolicus (strain ATCC BAA-128... [more]
BLAST of Carg04452 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1BYW91.7e-9383.73sirohydrochlorin ferrochelatase, chloroplastic OS=Momordica charantia OX=3673 GN... [more]
A0A5D3BY551.5e-9182.86Sirohydrochlorin ferrochelatase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A1S3C8S91.5e-9182.86sirohydrochlorin ferrochelatase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A6J1K3842.0e-9182.55sirohydrochlorin ferrochelatase, chloroplastic-like OS=Cucurbita maxima OX=3661 ... [more]
A0A6J1FHT51.3e-9081.60sirohydrochlorin ferrochelatase, chloroplastic-like OS=Cucurbita moschata OX=366... [more]
BLAST of Carg04452 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G50170.11.1e-6061.75sirohydrochlorin ferrochelatase B [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002762Sirohydrochlorin cobaltochelatase CbiX-likePFAMPF01903CbiXcoord: 77..181
e-value: 3.3E-29
score: 101.3
NoneNo IPR availableGENE3D3.40.50.1400coord: 68..191
e-value: 6.8E-34
score: 118.8
NoneNo IPR availablePANTHERPTHR33542SIROHYDROCHLORIN FERROCHELATASE, CHLOROPLASTICcoord: 4..207
NoneNo IPR availablePANTHERPTHR33542:SF3SIROHYDROCHLORIN FERROCHELATASE, CHLOROPLASTICcoord: 4..207
NoneNo IPR availableCDDcd03416CbiX_SirB_Ncoord: 71..171
e-value: 3.81266E-37
score: 123.066
NoneNo IPR availableSUPERFAMILY53800Chelatasecoord: 70..187

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg04452-RACarg04452-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006979 response to oxidative stress
biological_process GO:0019354 siroheme biosynthetic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0042802 identical protein binding
molecular_function GO:0051536 iron-sulfur cluster binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0051266 sirohydrochlorin ferrochelatase activity
molecular_function GO:0016829 lyase activity