Carg04432 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg04432
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	protease Do-like 1, chloroplastic
Location	Carg_Chr04: 20427860 .. 20432768 (-)
RNA-Seq Expression	Carg04432
Synteny	Carg04432

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTGAGCGATCCAATTTGCGTCAACTGTCCAAGCAGAAAAGTGATGGCTGCTGCATTTTCCCTCGCTTCCACTTTCTTCCATTTCTCCCCACTTTCTCGGCCTCCAAACGGAAGACCCACTTCCTTCTTTCTCTCTAAATCCATCCCTTTTCACAATTTCTCGAACCCCAGCTACCATTTTAGATACCCCATCTTCTCCCTTCTCGGCCACAACAAGAAGCGCAATCAAATTTCAGCTACGACTGACACAAAACTCGCCAATCCCTCGAACCCCTTAGCATCAATTTGCGAGTCGCTGCTAATTTTCACCACTTCGGTGTTCTTGTCCTTTTCTCTCTTTGTTACAGATGTTGATCCGGCGGCTGCTTTTGTTGTCACCACTCCTAGAAAATTGCAGACGGATGAGCTCGCAACGGTTCGTCTCTTTCAGGAGAATACTCCTTCTGTAGTTTATATCACAAATCTTGCTGCAAGGTAATTGTCCTTTTGGATTTGAGTTCTGATTATAACTTGGGTCGTTTCTTGGAGGTCTAATTTTGGTGCGATTGTCAATTTGAATGTGATTTTGATGTGGGTTTAGGCAGGATGCTTTTACACTAGACGTGTTGGAGGTACCACAAGGATCGGGTTCCGGATTTGTGTGGGATAAAGATGGAAATATTGTCACCAATTACCATGTAATTCGTGGTGCTTCTGATCTCAGGTTAGTCATGAACGCTCTTTAGTGACTTTATGTATAACTTCCATGGTATTCATTTGTGTGTTCATTACCTTGGGGGCTTTATCCATTTAAGGCTGTGGTATTAATAGGATATAAGCATCATCAAAGTACTTAATTGTAGAACCAATATGTTGCAAATGGTCCTCAGAAAATGCTTTCAGAAATGTGCGGTAACAGCCAAGCCCACGGCTAGATATTGTCATTTTTGGGCTTTCCCTTGTGAGCTTCCCCTCAAGGCTTTAAAACGCGTCCACTAGGGAGAGGATTCCACACCCTTATAAGGAATGCTTCATTCACCGCTCCAACCGATGTGGGATCTCACAATCCACCCCCCTTGGGGGCCCAGTGTCCTTGTTGGCACACCACCCAATGTCTAGCTCTGATACCATTTGTAACAGCACAAGTCCACCGCTAGCAGATATTGTCCTCTTTGGGCTTTTCCTTTCGGGCTTTTCCTCAAGGTTTTAAAACGCGTTCACTAAGGAGAGGTTTCCACACCCTTATAAGGAATGTTTTGTTCCTCTCTCCAACCAATGTGGGATCTCACGATCCACCCCCTTGCGGGCCCAACGTCCTCGCTGGCACACCACCTGGTGTCTAGCTCTGATACCATTTGTAACAGCACAAGTCCACCGCTAGCAGATATTGTCCTCTTTGGGCTTTTCCTTCCGGGCTTTCCCTCAAGGTTTTAAAACGCGTTCACTAGGGAGAGGTTTCCACACCCGTATAAGGAATGTTTCGTTCCTTTCTCCAACCGATGTGGGATCTCACGATCCACCCCCCTTGGGGGCCCAACGTCCTCGCTGGCACACCACTTGATGTCTAGCTTTGATACCATTTGTAACAGCCCAAGCCGTTCGCTAGCAAATACTGTCCTCTTTGGGCTTCCCCTTCCAGCCTTTTCCTCAAGGTTTTAAAATGCGTCCACCAGGAAGAGGTTTCAAGAGGTTTCCCCTCTCCAACCGACGTGGGATCTTACATGTGCAGCCACATTCGGCATAACTGTGTCATATTGTTTGGCTATGTGATTTTGTTTTCCTGGTTATGTATTGTGAGTAATGTCTTTGAAATGTATATTCTATAACTCTTTTTGCCTCGGGAACATGAAAAGAATAGCATCAAGTTCTTTTATTATTTGTAATGAGTTAAAGTTTTCTATTTGAATTTAGACTTAAAACTTAGTGTGGCACCTCATTATTCACATTTAAACTGGTTTTAGTTTTGTACAGAGTCACTCTAGCTGACCAAACTACTTTCGATGCAAAAGTTGTTGGATTCGACCAAGACAAGGATGTTGCTGTCTTGCGAATCGATGCACCAAAAGACAAACTAAGACCTATACCTGTTGGTATCTCAGCAGACTTGCTTGTTGGTCAGAAAGTTTTTGCTATTGGAAACCCTGTGAGTTTTTCTATTCACTGCCTTAGCCATTGGGTTTATGGTGCCCTTTGTCATGTTCATACATCTAATATATAGAAGTTTAGGCTACTAATTTCTTATGTAAAAAAATGAATAATCTAGGATGAAGCATTTTCTGCAAAGAGTATTCACTATTAACAAGATTCTAAAAAATTTTGCTGTTGGTAATTGCACAACACTCCTCTGATACTTGTGTATATGTAATGGTGTCATCTAAGAAGTTAGTTGGAACAGTCTAAGCCCACCGTCAGCAGGTATTGTCCTCTTTGGACTTTCCCTTCCGAGCTTCCCCTCAAGGTTTTAAATGGGTCTGCTAGAGAGAAGTTTCCACACCTTTATAAGGAATGCTTTGTTCTCCTCTCCAACCAATGTTGGATCTCACAATCTACCCCCCTTGGGGGCCCAGTGTCCCCGCTGACACACTACTCGGTGTTTGGCTTTGATACCATTTGTAGCAGTCCAAGCTATCGCTAGCCGATATTGTCCTCTTTGGACTTTACCTTCTGGGCTTCCCCACAAGGTTTTAAAACGTGTCTGCTAGGGAGAGGTTTTCACACCTTGATAAGGAATGTTTTGTTCTCCTCTCCAACCAACGTGGGATCTCATAAGCCACCCCTCTTGGGGCCCAGCATCCCTGCTAGCACACTACTCAGTGCCTGGCTCTGATACCATTTGTAACCGCCTAAGCCCACCGCCAACATGTATTGTCATCTTTGGGCTTTCCCTTCTAAGCTTCCTCTTAAAGTTTTAAAACGTGTCTGCTAGGGAGAGGTTTCCACACCCTTATAAGGAACGCTTCGTTCCCCTCTCCAACCAATGTGGGATCTTACATTAGTCAAAAGAGCACTGCAACTCGAAGCAAAGATTTTGGCATCCCAGGTCTTGGGCTTCACATATAAAAACATACACATTTTTGTATTAGAAAAAGAACTCGGGTTTAGATCATCATTTTTCCATTGGCTCTAAAGCCAGTCTCATCATGGTAGCTTCTTTATTGTCTTGTACATTTCCCCATTTGATGTCCTAAATATAATCATTATTGTCGATGCAGTTCGGACTTGATCATACTCTTACGACTGGGGTAATCAGGTATGAGACCCCACAGATTTGACCATACACTATATTAATATATCATATACTAAGCATTCATCTTGCTCTTGACAGTGGCCTTCGCAGAGAAATTAGTTCTGCTGCTACTGGTAGGCCCATTCAAGATGTAATTCAGACAGATGCTGCCATTAATCCAGGAAACAGTGGAGGGCCACTTCTCGATAGCTCGGGAAACCTCATCGGAATAAACACAGCAATATATTCTCCATCTGGGGCATCCTCAGGTGTTGGATTTTCAATCCCAGTTGACACTGTTAGTAATAAACATATGACATATCTTACAAGCTGCTGAAGTAACCTTGATTTGAAGAAGTATTCATCAACATTCTGTCAATGTAGGTGAGCGGCATTGTTGATCAGTTGGTGCGATATGGGAAGGTGACAAGACCGATATTAGGAATCAAGTTTGCTCCCGATCAGTCTGTGGAACAGTTGGGCGTAAGCGGAGTGCTTGTTTTGGATGCTCCTGCAAATGGTCCTGCTGGGAAAGCTGTATTAGTTTCCTCTCTTTTTCTCTCCGCATTTCTATTCTAATACCTATAGCGTTTCATTGTTCCATTATAGCTCCAAGAACTTGAAAAGACCTCCAACCTCAATACTTAAGTTCAAGAAGATTTGATCACTTCACATTATGCAGGGCCTGCTACCGACTAAGAGGGATGCTTATGGTAGACTTATATTGGGAGACATAATAACGTCGGTGAACGGGAAGAAGGTCGCGAATGGAAGCGATTTGTACAGAATTCTTGACCAGTGTAAAGTTGGTGACAAGGTAATCCAAACTTTCCTATTTCATCTTTTTTCTTTAGCTTGTTGAGCTCAAGGCATTTGTAACAACCCAAGCTCATTGCTAGCAAATATTGTCCTCTTTGGACTTTGTCTTTCGAGTTCCCCCTCAAGATTTTAAAACGCGTCTACTAGAGAGAGGTTTCCACACTCTGTTTCGTTCCCCTCTCCAACTTACGTGAGATCTCACACTTCACCCCTTGAATGTATATAAGGGCATGCCAAAAAGAAACGCCCACCATAAGAGTCCCCAAACAACCTACATGAACAAATTCCAATCCAAAAGAAATAGGGAGAGGTCTAGAATGACTTGGAGAATATTAATGAAATTTCTCCAAAACTCTGGGTTCTGAAAAAGACAGCAAGACCCCATGTGATGAAGCCAACTACCTTAGGATATGTTGCCTCATGATCCCCATGTGGCCTTTTAGTGACAATACAAAGCCATTTGGTCTCATAATTTTCCAGAAAAATTATTTTAACCACTTTTAAACCCAAAAGTTCTCGATTTAGTCCCTAAACTATGTATAAAAAGTCATGTATACGATACCGTTTCAAGAATTAGAAACGGATTTTCACCTGAAAAATGTAGTATAACCAAATAAAAGACTCATGGACTGCAAAGAGAATGATCCCAGTAACATAAACCATTGATGAACTTGAATTCTCAAGAAGTATTCAAATTTTGTGACATGTTTTCATGGAGGTTTGATTGGTAACCAATTGTTTGATGCTTGCTCAGGTGATTGTGGAGGTGCTACGTGGTGATCACATGGAGAAAATTCCTGTAATTCTGGAGCCAAAGCCTGACGAATCTTGAGCCAAAAATAGTTGGACATTGTTTGTATTTTTGTACATTACATTAATCAATATC

mRNA sequence

TTGAGCGATCCAATTTGCGTCAACTGTCCAAGCAGAAAAGTGATGGCTGCTGCATTTTCCCTCGCTTCCACTTTCTTCCATTTCTCCCCACTTTCTCGGCCTCCAAACGGAAGACCCACTTCCTTCTTTCTCTCTAAATCCATCCCTTTTCACAATTTCTCGAACCCCAGCTACCATTTTAGATACCCCATCTTCTCCCTTCTCGGCCACAACAAGAAGCGCAATCAAATTTCAGCTACGACTGACACAAAACTCGCCAATCCCTCGAACCCCTTAGCATCAATTTGCGAGTCGCTGCTAATTTTCACCACTTCGGTGTTCTTGTCCTTTTCTCTCTTTGTTACAGATGTTGATCCGGCGGCTGCTTTTGTTGTCACCACTCCTAGAAAATTGCAGACGGATGAGCTCGCAACGGTTCGTCTCTTTCAGGAGAATACTCCTTCTGTAGTTTATATCACAAATCTTGCTGCAAGGCAGGATGCTTTTACACTAGACGTGTTGGAGGTACCACAAGGATCGGGTTCCGGATTTGTGTGGGATAAAGATGGAAATATTGTCACCAATTACCATGTAATTCGTGGTGCTTCTGATCTCAGAGTCACTCTAGCTGACCAAACTACTTTCGATGCAAAAGTTGTTGGATTCGACCAAGACAAGGATGTTGCTGTCTTGCGAATCGATGCACCAAAAGACAAACTAAGACCTATACCTGTTGGTATCTCAGCAGACTTGCTTGTTGGTCAGAAAGTTTTTGCTATTGGAAACCCTTTCGGACTTGATCATACTCTTACGACTGGGGTAATCAGTGGCCTTCGCAGAGAAATTAGTTCTGCTGCTACTGGTAGGCCCATTCAAGATGTAATTCAGACAGATGCTGCCATTAATCCAGGAAACAGTGGAGGGCCACTTCTCGATAGCTCGGGAAACCTCATCGGAATAAACACAGCAATATATTCTCCATCTGGGGCATCCTCAGGTGTTGGATTTTCAATCCCAGTTGACACTGTGAGCGGCATTGTTGATCAGTTGGTGCGATATGGGAAGGTGACAAGACCGATATTAGGAATCAAGTTTGCTCCCGATCAGTCTGTGGAACAGTTGGGCGTAAGCGGAGTGCTTGTTTTGGATGCTCCTGCAAATGGTCCTGCTGGGAAAGCTGGCCTGCTACCGACTAAGAGGGATGCTTATGGTAGACTTATATTGGGAGACATAATAACGTCGGTGAACGGGAAGAAGGTCGCGAATGGAAGCGATTTGTACAGAATTCTTGACCAGTGTAAAGTTGGTGACAAGGTGATTGTGGAGGTGCTACGTGGTGATCACATGGAGAAAATTCCTGTAATTCTGGAGCCAAAGCCTGACGAATCTTGAGCCAAAAATAGTTGGACATTGTTTGTATTTTTGTACATTACATTAATCAATATC

Coding sequence (CDS)

ATGGCTGCTGCATTTTCCCTCGCTTCCACTTTCTTCCATTTCTCCCCACTTTCTCGGCCTCCAAACGGAAGACCCACTTCCTTCTTTCTCTCTAAATCCATCCCTTTTCACAATTTCTCGAACCCCAGCTACCATTTTAGATACCCCATCTTCTCCCTTCTCGGCCACAACAAGAAGCGCAATCAAATTTCAGCTACGACTGACACAAAACTCGCCAATCCCTCGAACCCCTTAGCATCAATTTGCGAGTCGCTGCTAATTTTCACCACTTCGGTGTTCTTGTCCTTTTCTCTCTTTGTTACAGATGTTGATCCGGCGGCTGCTTTTGTTGTCACCACTCCTAGAAAATTGCAGACGGATGAGCTCGCAACGGTTCGTCTCTTTCAGGAGAATACTCCTTCTGTAGTTTATATCACAAATCTTGCTGCAAGGCAGGATGCTTTTACACTAGACGTGTTGGAGGTACCACAAGGATCGGGTTCCGGATTTGTGTGGGATAAAGATGGAAATATTGTCACCAATTACCATGTAATTCGTGGTGCTTCTGATCTCAGAGTCACTCTAGCTGACCAAACTACTTTCGATGCAAAAGTTGTTGGATTCGACCAAGACAAGGATGTTGCTGTCTTGCGAATCGATGCACCAAAAGACAAACTAAGACCTATACCTGTTGGTATCTCAGCAGACTTGCTTGTTGGTCAGAAAGTTTTTGCTATTGGAAACCCTTTCGGACTTGATCATACTCTTACGACTGGGGTAATCAGTGGCCTTCGCAGAGAAATTAGTTCTGCTGCTACTGGTAGGCCCATTCAAGATGTAATTCAGACAGATGCTGCCATTAATCCAGGAAACAGTGGAGGGCCACTTCTCGATAGCTCGGGAAACCTCATCGGAATAAACACAGCAATATATTCTCCATCTGGGGCATCCTCAGGTGTTGGATTTTCAATCCCAGTTGACACTGTGAGCGGCATTGTTGATCAGTTGGTGCGATATGGGAAGGTGACAAGACCGATATTAGGAATCAAGTTTGCTCCCGATCAGTCTGTGGAACAGTTGGGCGTAAGCGGAGTGCTTGTTTTGGATGCTCCTGCAAATGGTCCTGCTGGGAAAGCTGGCCTGCTACCGACTAAGAGGGATGCTTATGGTAGACTTATATTGGGAGACATAATAACGTCGGTGAACGGGAAGAAGGTCGCGAATGGAAGCGATTTGTACAGAATTCTTGACCAGTGTAAAGTTGGTGACAAGGTGATTGTGGAGGTGCTACGTGGTGATCACATGGAGAAAATTCCTGTAATTCTGGAGCCAAAGCCTGACGAATCTTGA

Protein sequence

MAAAFSLASTFFHFSPLSRPPNGRPTSFFLSKSIPFHNFSNPSYHFRYPIFSLLGHNKKRNQISATTDTKLANPSNPLASICESLLIFTTSVFLSFSLFVTDVDPAAAFVVTTPRKLQTDELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRGASDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIGNPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTAIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVEQLGVSGVLVLDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVANGSDLYRILDQCKVGDKVIVEVLRGDHMEKIPVILEPKPDES

Homology

[Close]

BLAST of Carg04432 vs. NCBI nr
Match: KAG7033121.1 (Protease Do-like 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 862.1 bits (2226), Expect = 2.1e-246
Identity = 442/442 (100.00%), Postives = 442/442 (100.00%), Query Frame = 0


Query: 1   MAAAFSLASTFFHFSPLSRPPNGRPTSFFLSKSIPFHNFSNPSYHFRYPIFSLLGHNKKR 60
           MAAAFSLASTFFHFSPLSRPPNGRPTSFFLSKSIPFHNFSNPSYHFRYPIFSLLGHNKKR
Sbjct: 1   MAAAFSLASTFFHFSPLSRPPNGRPTSFFLSKSIPFHNFSNPSYHFRYPIFSLLGHNKKR 60

Query: 61  NQISATTDTKLANPSNPLASICESLLIFTTSVFLSFSLFVTDVDPAAAFVVTTPRKLQTD 120
           NQISATTDTKLANPSNPLASICESLLIFTTSVFLSFSLFVTDVDPAAAFVVTTPRKLQTD
Sbjct: 61  NQISATTDTKLANPSNPLASICESLLIFTTSVFLSFSLFVTDVDPAAAFVVTTPRKLQTD 120

Query: 121 ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRG 180
           ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRG
Sbjct: 121 ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRG 180

Query: 181 ASDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIG 240
           ASDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIG
Sbjct: 181 ASDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIG 240

Query: 241 NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN 300
           NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN
Sbjct: 241 NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN 300

Query: 301 TAIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVEQLGVSGVLV 360
           TAIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVEQLGVSGVLV
Sbjct: 301 TAIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVEQLGVSGVLV 360

Query: 361 LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVANGSDLYRILDQCKVGDKVIV 420
           LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVANGSDLYRILDQCKVGDKVIV
Sbjct: 361 LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVANGSDLYRILDQCKVGDKVIV 420

Query: 421 EVLRGDHMEKIPVILEPKPDES 443
           EVLRGDHMEKIPVILEPKPDES
Sbjct: 421 EVLRGDHMEKIPVILEPKPDES 442

[Close]

BLAST of Carg04432 vs. NCBI nr
Match: XP_022922573.1 (protease Do-like 1, chloroplastic [Cucurbita moschata] >KAG6602439.1 Protease Do-like 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 860.5 bits (2222), Expect = 6.2e-246
Identity = 441/442 (99.77%), Postives = 441/442 (99.77%), Query Frame = 0


Query: 1   MAAAFSLASTFFHFSPLSRPPNGRPTSFFLSKSIPFHNFSNPSYHFRYPIFSLLGHNKKR 60
           MAAAFSLASTFFHFSPLSRPPNGRPTSFFLSKSIPFHNFSNPSYHFRYPIFSLLGHNKKR
Sbjct: 1   MAAAFSLASTFFHFSPLSRPPNGRPTSFFLSKSIPFHNFSNPSYHFRYPIFSLLGHNKKR 60

Query: 61  NQISATTDTKLANPSNPLASICESLLIFTTSVFLSFSLFVTDVDPAAAFVVTTPRKLQTD 120
           NQISATTDTKL NPSNPLASICESLLIFTTSVFLSFSLFVTDVDPAAAFVVTTPRKLQTD
Sbjct: 61  NQISATTDTKLTNPSNPLASICESLLIFTTSVFLSFSLFVTDVDPAAAFVVTTPRKLQTD 120

Query: 121 ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRG 180
           ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRG
Sbjct: 121 ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRG 180

Query: 181 ASDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIG 240
           ASDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIG
Sbjct: 181 ASDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIG 240

Query: 241 NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN 300
           NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN
Sbjct: 241 NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN 300

Query: 301 TAIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVEQLGVSGVLV 360
           TAIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVEQLGVSGVLV
Sbjct: 301 TAIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVEQLGVSGVLV 360

Query: 361 LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVANGSDLYRILDQCKVGDKVIV 420
           LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVANGSDLYRILDQCKVGDKVIV
Sbjct: 361 LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVANGSDLYRILDQCKVGDKVIV 420

Query: 421 EVLRGDHMEKIPVILEPKPDES 443
           EVLRGDHMEKIPVILEPKPDES
Sbjct: 421 EVLRGDHMEKIPVILEPKPDES 442

[Close]

BLAST of Carg04432 vs. NCBI nr
Match: XP_023515810.1 (protease Do-like 1, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 854.7 bits (2207), Expect = 3.4e-244
Identity = 438/442 (99.10%), Postives = 439/442 (99.32%), Query Frame = 0


Query: 1   MAAAFSLASTFFHFSPLSRPPNGRPTSFFLSKSIPFHNFSNPSYHFRYPIFSLLGHNKKR 60
           MAAAFSLASTFFHFSPLSRPPNGRPTSFFLSKSIPFHNFSNPS HFRYPIFS+LGHNKKR
Sbjct: 1   MAAAFSLASTFFHFSPLSRPPNGRPTSFFLSKSIPFHNFSNPSCHFRYPIFSILGHNKKR 60

Query: 61  NQISATTDTKLANPSNPLASICESLLIFTTSVFLSFSLFVTDVDPAAAFVVTTPRKLQTD 120
           NQISATTDTKL NPSNP ASICESLLIFTTSVFLSFSLFVTDVDPAAAFVVTTPRKLQTD
Sbjct: 61  NQISATTDTKLTNPSNPFASICESLLIFTTSVFLSFSLFVTDVDPAAAFVVTTPRKLQTD 120

Query: 121 ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRG 180
           ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRG
Sbjct: 121 ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRG 180

Query: 181 ASDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIG 240
           ASDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIG
Sbjct: 181 ASDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIG 240

Query: 241 NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN 300
           NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN
Sbjct: 241 NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN 300

Query: 301 TAIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVEQLGVSGVLV 360
           TAIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVEQLGVSGVLV
Sbjct: 301 TAIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVEQLGVSGVLV 360

Query: 361 LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVANGSDLYRILDQCKVGDKVIV 420
           LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVANGSDLYRILDQCKVGDKVIV
Sbjct: 361 LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVANGSDLYRILDQCKVGDKVIV 420

Query: 421 EVLRGDHMEKIPVILEPKPDES 443
           EVLRGDHMEKIPVILEPKPDES
Sbjct: 421 EVLRGDHMEKIPVILEPKPDES 442

[Close]

BLAST of Carg04432 vs. NCBI nr
Match: XP_022990583.1 (protease Do-like 1, chloroplastic [Cucurbita maxima])

HSP 1 Score: 850.1 bits (2195), Expect = 8.4e-243
Identity = 436/442 (98.64%), Postives = 437/442 (98.87%), Query Frame = 0


Query: 1   MAAAFSLASTFFHFSPLSRPPNGRPTSFFLSKSIPFHNFSNPSYHFRYPIFSLLGHNKKR 60
           MAAAFSLASTFFHFSPLSRPPNGRPTSFFLSKSIPFHNFSNPS HFRYPIFSLLGH KKR
Sbjct: 1   MAAAFSLASTFFHFSPLSRPPNGRPTSFFLSKSIPFHNFSNPSCHFRYPIFSLLGHTKKR 60

Query: 61  NQISATTDTKLANPSNPLASICESLLIFTTSVFLSFSLFVTDVDPAAAFVVTTPRKLQTD 120
           NQISATTDTKL NPSNP ASICESLLIFTTSVFLSFSLFVTDVDPAAAFVVTTPRKLQTD
Sbjct: 61  NQISATTDTKLTNPSNPFASICESLLIFTTSVFLSFSLFVTDVDPAAAFVVTTPRKLQTD 120

Query: 121 ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRG 180
           ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRG
Sbjct: 121 ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRG 180

Query: 181 ASDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIG 240
           ASDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIG
Sbjct: 181 ASDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIG 240

Query: 241 NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN 300
           NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN
Sbjct: 241 NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN 300

Query: 301 TAIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVEQLGVSGVLV 360
           TAIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVEQLGVSGVLV
Sbjct: 301 TAIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVEQLGVSGVLV 360

Query: 361 LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVANGSDLYRILDQCKVGDKVIV 420
           LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKV NGSDLYRILDQCKVG+KVIV
Sbjct: 361 LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGEKVIV 420

Query: 421 EVLRGDHMEKIPVILEPKPDES 443
           EVLRGDHMEKIPVILEPKPDES
Sbjct: 421 EVLRGDHMEKIPVILEPKPDES 442

[Close]

BLAST of Carg04432 vs. NCBI nr
Match: XP_023550169.1 (protease Do-like 1, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 787.3 bits (2032), Expect = 6.7e-224
Identity = 409/442 (92.53%), Postives = 416/442 (94.12%), Query Frame = 0


Query: 1   MAAAFSLASTFFHFSPLSRPPNGRPTSFFLSKSIPFHNFSNPSYHFRYPIFSLLGHNKKR 60
           MAAAFSLASTF HFSP SRPPN  PTSFFLSKSI  HNF+NPS H RYPI SLL HNK  
Sbjct: 1   MAAAFSLASTFSHFSPPSRPPNRTPTSFFLSKSISLHNFTNPSSHLRYPISSLLPHNKNH 60

Query: 61  NQISATTDTKLANPSNPLASICESLLIFTTSVFLSFSLFVTDVDPAAAFVVTTPRKLQTD 120
            QISATT TKL  PSNP ASI ESLLIF+TS  LSFSLF+TDVDPA AFVVTTPRKLQTD
Sbjct: 61  TQISATTHTKLPPPSNPFASIWESLLIFSTSALLSFSLFLTDVDPAVAFVVTTPRKLQTD 120

Query: 121 ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRG 180
           ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDG++VTNYHVIRG
Sbjct: 121 ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGHVVTNYHVIRG 180

Query: 181 ASDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIG 240
           ASDLRVTLADQTTFDAKVVGFDQDKDVAVL IDAPKDKLRPIPVGISADLLVGQKVFAIG
Sbjct: 181 ASDLRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIG 240

Query: 241 NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN 300
           NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN
Sbjct: 241 NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN 300

Query: 301 TAIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVEQLGVSGVLV 360
           TAIYSPSGASSGVGFSIPVDTVSGIVDQLVR+GKVTRPILGIKFAPDQSVEQLGVSGVLV
Sbjct: 301 TAIYSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLV 360

Query: 361 LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVANGSDLYRILDQCKVGDKVIV 420
           LDAPANGPAGKAGL  TKRDAYGRLILGDIITSV+GKKV NGSDLYRILDQCKVGDKV V
Sbjct: 361 LDAPANGPAGKAGLRSTKRDAYGRLILGDIITSVDGKKVMNGSDLYRILDQCKVGDKVTV 420

Query: 421 EVLRGDHMEKIPVILEPKPDES 443
           EVLRGDHMEKIPVILEPKPDES
Sbjct: 421 EVLRGDHMEKIPVILEPKPDES 442

[Close]

BLAST of Carg04432 vs. ExPASy Swiss-Prot
Match: O22609 (Protease Do-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP1 PE=1 SV=2)

HSP 1 Score: 624.8 bits (1610), Expect = 7.5e-178
Identity = 345/440 (78.41%), Postives = 366/440 (83.18%), Query Frame = 0


Query: 7   LASTFFHFSPLSRPPNGRPTSFFL--SKSIPFHNFSNPSYHFRYPIFSLLGHNKKRNQIS 66
           L+ST F    L  PP+   + F L  S+S P   +   S  + + I S L  N   N   
Sbjct: 11  LSSTLF----LHSPPSSHLSFFNLSSSRSSPISLYPIRSKRY-FRILSKLSLND--NNRD 70

Query: 67  ATTDTKLANPSNPLASICESLLIFTTSVFLSFSLFVTD--VDPAAAFVVTTPRKLQTDEL 126
              DT    P     S  +   +  TSV LSFSLF     V+ A+AFVV+TP+KLQTDEL
Sbjct: 71  DDDDTLHFTP----FSAVKPFFLLCTSVALSFSLFAASPAVESASAFVVSTPKKLQTDEL 130

Query: 127 ATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRGAS 186
           ATVRLFQENTPSVVYITNLA RQDAFTLDVLEVPQGSGSGFVWDK G+IVTNYHVIRGAS
Sbjct: 131 ATVRLFQENTPSVVYITNLAVRQDAFTLDVLEVPQGSGSGFVWDKQGHIVTNYHVIRGAS 190

Query: 187 DLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIGNP 246
           DLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPK+KLRPIPVG+SADLLVGQKVFAIGNP
Sbjct: 191 DLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKNKLRPIPVGVSADLLVGQKVFAIGNP 250

Query: 247 FGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTA 306
           FGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSG LIGINTA
Sbjct: 251 FGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGTLIGINTA 310

Query: 307 IYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVEQLGVSGVLVLD 366
           IYSPSGASSGVGFSIPVDTV GIVDQLVR+GKVTRPILGIKFAPDQSVEQLGVSGVLVLD
Sbjct: 311 IYSPSGASSGVGFSIPVDTVGGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLD 370

Query: 367 APANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVANGSDLYRILDQCKVGDKVIVEV 426
           AP +GPAGKAGL  TKRD YGRL+LGDIITSVNG KV+NGSDLYRILDQCKVGD+V VEV
Sbjct: 371 APPSGPAGKAGLQSTKRDGYGRLVLGDIITSVNGTKVSNGSDLYRILDQCKVGDEVTVEV 430

Query: 427 LRGDHMEKIPVILEPKPDES 443
           LRGDH EKI V LEPKPDES
Sbjct: 431 LRGDHKEKISVTLEPKPDES 439

[Close]

BLAST of Carg04432 vs. ExPASy Swiss-Prot
Match: Q9LU10 (Protease Do-like 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP8 PE=1 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 7.3e-80
Identity = 169/328 (51.52%), Postives = 215/328 (65.55%), Query Frame = 0


Query: 125 VRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRGASDL 184
           V+LF++NT SVV I ++  R       V+E+P+G+GSG VWD  G IVTNYHVI  A   
Sbjct: 120 VQLFEKNTYSVVNIFDVTLRPQLKMTGVVEIPEGNGSGVVWDGQGYIVTNYHVIGNALSR 179

Query: 185 RVTLAD-------------QTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLL 244
             +  D             Q  F+ K+VG D+ KD+AVL++DAP+  L+PI VG S  L 
Sbjct: 180 NPSPGDVVGRVNILASDGVQKNFEGKLVGADRAKDLAVLKVDAPETLLKPIKVGQSNSLK 239

Query: 245 VGQKVFAIGNPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLD 304
           VGQ+  AIGNPFG DHTLT GVISGL R+I S  TG  I   IQTDAAINPGNSGGPLLD
Sbjct: 240 VGQQCLAIGNPFGFDHTLTVGVISGLNRDIFS-QTGVTIGGGIQTDAAINPGNSGGPLLD 299

Query: 305 SSGNLIGINTAIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVE 364
           S GNLIGINTAI++ +G S+GVGF+IP  TV  IV QL+++ KV R  + I+ APD    
Sbjct: 300 SKGNLIGINTAIFTQTGTSAGVGFAIPSSTVLKIVPQLIQFSKVLRAGINIELAPDPVAN 359

Query: 365 QLGV-SGVLVLDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVANGSDLYRILD 424
           QL V +G LVL  P    A KAGL PT R   G ++LGDII +V+ K V N ++L +ILD
Sbjct: 360 QLNVRNGALVLQVPGKSLAEKAGLHPTSRGFAGNIVLGDIIVAVDDKPVKNKAELMKILD 419

Query: 425 QCKVGDKVIVEVLRGDHMEKIPVILEPK 439
           +  VGDKV +++ RG+   ++ + LE K
Sbjct: 420 EYSVGDKVTLKIKRGNEDLELKISLEEK 446

[Close]

BLAST of Carg04432 vs. ExPASy Swiss-Prot
Match: Q2YMX6 (Probable periplasmic serine endoprotease DegP-like OS=Brucella abortus (strain 2308) OX=359391 GN=htrA PE=3 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 8.4e-52
Identity = 121/293 (41.30%), Postives = 166/293 (56.66%), Query Frame = 0


Query: 154 EVPQGSGSGFVWDKDGNIVTNYHVIRGASDLRVTLADQTTFDAKVVGFDQDKDVAVLRID 213
           E P   GSGFV  +DG +VTN HV+       V L D T  DAK++G D   D+AVL+I+
Sbjct: 130 ERPVAQGSGFVISEDGYVVTNNHVVSDGDAYTVVLDDGTELDAKLIGADPRTDLAVLKIN 189

Query: 214 APKDKLRPIPVGISADLLVGQKVFAIGNPFGLDHTLTTGVISGLRREISSAATGRPIQDV 273
           APK K   +  G    + VG  V A+GNPFGL  T+T+G++S   R+I +     P  D 
Sbjct: 190 APKRKFVYVAFGDDNKVRVGDWVVAVGNPFGLGGTVTSGIVSARGRDIGAG----PYDDF 249

Query: 274 IQTDAAINPGNSGGPLLDSSGNLIGINTAIYSPSGASSGVGFSIPVDTVSGIVDQLVRYG 333
           IQ DAA+N GNSGGP  D SG +IGINTAI+SPSG S G+ F+IP  T   +VDQL++ G
Sbjct: 250 IQIDAAVNKGNSGGPAFDLSGEVIGINTAIFSPSGGSVGIAFAIPSSTAKQVVDQLIKKG 309

Query: 334 KVTRPILGIKFAP--DQSVEQLGVS---GVLVLDAPANGPAGKAGLLPTKRDAYGRLILG 393
            V R  +G++  P        LG++   G +V     +GPA KAG+             G
Sbjct: 310 SVERGWIGVQIQPVTKDIAASLGLAEEKGAIVASPQDDGPAAKAGIK-----------AG 369

Query: 394 DIITSVNGKKVANGSDLYRILDQCKVGDKVIVEVLRGDHMEKIPVILEPKPDE 442
           D+IT+VNG+ V +  DL R +     G+K  + V R +  E+I V +   P++
Sbjct: 370 DVITAVNGETVQDPRDLARKVANIAPGEKAALTVWRKNKAEEINVTIAAMPND 407

[Close]

BLAST of Carg04432 vs. ExPASy Swiss-Prot
Match: P0C114 (Probable periplasmic serine endoprotease DegP-like OS=Brucella abortus biovar 1 (strain 9-941) OX=262698 GN=htrA PE=3 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 8.4e-52
Identity = 121/293 (41.30%), Postives = 166/293 (56.66%), Query Frame = 0


Query: 154 EVPQGSGSGFVWDKDGNIVTNYHVIRGASDLRVTLADQTTFDAKVVGFDQDKDVAVLRID 213
           E P   GSGFV  +DG +VTN HV+       V L D T  DAK++G D   D+AVL+I+
Sbjct: 130 ERPVAQGSGFVISEDGYVVTNNHVVSDGDAYTVVLDDGTELDAKLIGADPRTDLAVLKIN 189

Query: 214 APKDKLRPIPVGISADLLVGQKVFAIGNPFGLDHTLTTGVISGLRREISSAATGRPIQDV 273
           APK K   +  G    + VG  V A+GNPFGL  T+T+G++S   R+I +     P  D 
Sbjct: 190 APKRKFVYVAFGDDNKVRVGDWVVAVGNPFGLGGTVTSGIVSARGRDIGAG----PYDDF 249

Query: 274 IQTDAAINPGNSGGPLLDSSGNLIGINTAIYSPSGASSGVGFSIPVDTVSGIVDQLVRYG 333
           IQ DAA+N GNSGGP  D SG +IGINTAI+SPSG S G+ F+IP  T   +VDQL++ G
Sbjct: 250 IQIDAAVNKGNSGGPAFDLSGEVIGINTAIFSPSGGSVGIAFAIPSSTAKQVVDQLIKKG 309

Query: 334 KVTRPILGIKFAP--DQSVEQLGVS---GVLVLDAPANGPAGKAGLLPTKRDAYGRLILG 393
            V R  +G++  P        LG++   G +V     +GPA KAG+             G
Sbjct: 310 SVERGWIGVQIQPVTKDIAASLGLAEEKGAIVASPQDDGPAAKAGIK-----------AG 369

Query: 394 DIITSVNGKKVANGSDLYRILDQCKVGDKVIVEVLRGDHMEKIPVILEPKPDE 442
           D+IT+VNG+ V +  DL R +     G+K  + V R +  E+I V +   P++
Sbjct: 370 DVITAVNGETVQDPRDLARKVANIAPGEKAALTVWRKNKAEEINVTIAAMPND 407

[Close]

BLAST of Carg04432 vs. ExPASy Swiss-Prot
Match: Q8YG32 (Probable periplasmic serine endoprotease DegP-like OS=Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) OX=224914 GN=htrA PE=3 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 8.4e-52
Identity = 121/293 (41.30%), Postives = 166/293 (56.66%), Query Frame = 0


Query: 154 EVPQGSGSGFVWDKDGNIVTNYHVIRGASDLRVTLADQTTFDAKVVGFDQDKDVAVLRID 213
           E P   GSGFV  +DG +VTN HV+       V L D T  DAK++G D   D+AVL+I+
Sbjct: 130 ERPVAQGSGFVISEDGYVVTNNHVVSDGDAYTVVLDDGTELDAKLIGADPRTDLAVLKIN 189

Query: 214 APKDKLRPIPVGISADLLVGQKVFAIGNPFGLDHTLTTGVISGLRREISSAATGRPIQDV 273
           APK K   +  G    + VG  V A+GNPFGL  T+T+G++S   R+I +     P  D 
Sbjct: 190 APKRKFVYVAFGDDNKVRVGDWVVAVGNPFGLGGTVTSGIVSARGRDIGAG----PYDDF 249

Query: 274 IQTDAAINPGNSGGPLLDSSGNLIGINTAIYSPSGASSGVGFSIPVDTVSGIVDQLVRYG 333
           IQ DAA+N GNSGGP  D SG +IGINTAI+SPSG S G+ F+IP  T   +VDQL++ G
Sbjct: 250 IQIDAAVNKGNSGGPAFDLSGEVIGINTAIFSPSGGSVGIAFAIPSSTAKQVVDQLIKKG 309

Query: 334 KVTRPILGIKFAP--DQSVEQLGVS---GVLVLDAPANGPAGKAGLLPTKRDAYGRLILG 393
            V R  +G++  P        LG++   G +V     +GPA KAG+             G
Sbjct: 310 SVERGWIGVQIQPVTKDIAASLGLAEEKGAIVASPQDDGPAAKAGIK-----------AG 369

Query: 394 DIITSVNGKKVANGSDLYRILDQCKVGDKVIVEVLRGDHMEKIPVILEPKPDE 442
           D+IT+VNG+ V +  DL R +     G+K  + V R +  E+I V +   P++
Sbjct: 370 DVITAVNGETVQDPRDLARKVANIAPGEKAALTVWRKNKAEEINVTIAAMPND 407

[Close]

BLAST of Carg04432 vs. ExPASy TrEMBL
Match: A0A6J1E723 (protease Do-like 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111430538 PE=3 SV=1)

HSP 1 Score: 860.5 bits (2222), Expect = 3.0e-246
Identity = 441/442 (99.77%), Postives = 441/442 (99.77%), Query Frame = 0


Query: 1   MAAAFSLASTFFHFSPLSRPPNGRPTSFFLSKSIPFHNFSNPSYHFRYPIFSLLGHNKKR 60
           MAAAFSLASTFFHFSPLSRPPNGRPTSFFLSKSIPFHNFSNPSYHFRYPIFSLLGHNKKR
Sbjct: 1   MAAAFSLASTFFHFSPLSRPPNGRPTSFFLSKSIPFHNFSNPSYHFRYPIFSLLGHNKKR 60

Query: 61  NQISATTDTKLANPSNPLASICESLLIFTTSVFLSFSLFVTDVDPAAAFVVTTPRKLQTD 120
           NQISATTDTKL NPSNPLASICESLLIFTTSVFLSFSLFVTDVDPAAAFVVTTPRKLQTD
Sbjct: 61  NQISATTDTKLTNPSNPLASICESLLIFTTSVFLSFSLFVTDVDPAAAFVVTTPRKLQTD 120

Query: 121 ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRG 180
           ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRG
Sbjct: 121 ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRG 180

Query: 181 ASDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIG 240
           ASDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIG
Sbjct: 181 ASDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIG 240

Query: 241 NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN 300
           NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN
Sbjct: 241 NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN 300

Query: 301 TAIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVEQLGVSGVLV 360
           TAIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVEQLGVSGVLV
Sbjct: 301 TAIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVEQLGVSGVLV 360

Query: 361 LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVANGSDLYRILDQCKVGDKVIV 420
           LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVANGSDLYRILDQCKVGDKVIV
Sbjct: 361 LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVANGSDLYRILDQCKVGDKVIV 420

Query: 421 EVLRGDHMEKIPVILEPKPDES 443
           EVLRGDHMEKIPVILEPKPDES
Sbjct: 421 EVLRGDHMEKIPVILEPKPDES 442

[Close]

BLAST of Carg04432 vs. ExPASy TrEMBL
Match: A0A6J1JTP9 (protease Do-like 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111487418 PE=3 SV=1)

HSP 1 Score: 850.1 bits (2195), Expect = 4.1e-243
Identity = 436/442 (98.64%), Postives = 437/442 (98.87%), Query Frame = 0


Query: 1   MAAAFSLASTFFHFSPLSRPPNGRPTSFFLSKSIPFHNFSNPSYHFRYPIFSLLGHNKKR 60
           MAAAFSLASTFFHFSPLSRPPNGRPTSFFLSKSIPFHNFSNPS HFRYPIFSLLGH KKR
Sbjct: 1   MAAAFSLASTFFHFSPLSRPPNGRPTSFFLSKSIPFHNFSNPSCHFRYPIFSLLGHTKKR 60

Query: 61  NQISATTDTKLANPSNPLASICESLLIFTTSVFLSFSLFVTDVDPAAAFVVTTPRKLQTD 120
           NQISATTDTKL NPSNP ASICESLLIFTTSVFLSFSLFVTDVDPAAAFVVTTPRKLQTD
Sbjct: 61  NQISATTDTKLTNPSNPFASICESLLIFTTSVFLSFSLFVTDVDPAAAFVVTTPRKLQTD 120

Query: 121 ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRG 180
           ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRG
Sbjct: 121 ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRG 180

Query: 181 ASDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIG 240
           ASDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIG
Sbjct: 181 ASDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIG 240

Query: 241 NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN 300
           NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN
Sbjct: 241 NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN 300

Query: 301 TAIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVEQLGVSGVLV 360
           TAIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVEQLGVSGVLV
Sbjct: 301 TAIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVEQLGVSGVLV 360

Query: 361 LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVANGSDLYRILDQCKVGDKVIV 420
           LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKV NGSDLYRILDQCKVG+KVIV
Sbjct: 361 LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGEKVIV 420

Query: 421 EVLRGDHMEKIPVILEPKPDES 443
           EVLRGDHMEKIPVILEPKPDES
Sbjct: 421 EVLRGDHMEKIPVILEPKPDES 442

[Close]

BLAST of Carg04432 vs. ExPASy TrEMBL
Match: A0A6J1FEU2 (protease Do-like 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111445066 PE=3 SV=1)

HSP 1 Score: 783.5 bits (2022), Expect = 4.7e-223
Identity = 407/442 (92.08%), Postives = 414/442 (93.67%), Query Frame = 0


Query: 1   MAAAFSLASTFFHFSPLSRPPNGRPTSFFLSKSIPFHNFSNPSYHFRYPIFSLLGHNKKR 60
           MAAAFSLASTF HFSP SRPPN  PT FFLSKSI   NF+NPS H RYPI SLL HNK  
Sbjct: 1   MAAAFSLASTFSHFSPPSRPPNRTPTPFFLSKSISLQNFTNPSSHLRYPICSLLPHNKNH 60

Query: 61  NQISATTDTKLANPSNPLASICESLLIFTTSVFLSFSLFVTDVDPAAAFVVTTPRKLQTD 120
            QISATT TKL  PSNP AS  ESLLIF+TS  LSFSLF+TDVDPA AFVVTTPRKLQTD
Sbjct: 61  TQISATTHTKLPPPSNPFASTWESLLIFSTSALLSFSLFLTDVDPAVAFVVTTPRKLQTD 120

Query: 121 ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRG 180
           ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDG++VTNYHVIRG
Sbjct: 121 ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGHVVTNYHVIRG 180

Query: 181 ASDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIG 240
           ASDLRVTLADQTTFDAKVVGFDQDKDVAVL IDAPKDKLRPIPVGISADLLVGQKVFAIG
Sbjct: 181 ASDLRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIG 240

Query: 241 NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN 300
           NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN
Sbjct: 241 NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN 300

Query: 301 TAIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVEQLGVSGVLV 360
           TAIYSPSGASSGVGFSIPVDTVSGIVDQLVR+GKVTRPILGIKFAPDQSVEQLGVSGVLV
Sbjct: 301 TAIYSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLV 360

Query: 361 LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVANGSDLYRILDQCKVGDKVIV 420
           LDAPANGPAGKAGL PTKRDAYGRLILGDIITSV+GKKV NGSDLYRILDQCKVGDKV V
Sbjct: 361 LDAPANGPAGKAGLRPTKRDAYGRLILGDIITSVDGKKVMNGSDLYRILDQCKVGDKVTV 420

Query: 421 EVLRGDHMEKIPVILEPKPDES 443
           EVLRGDHMEKIPVILEPKPDES
Sbjct: 421 EVLRGDHMEKIPVILEPKPDES 442

[Close]

BLAST of Carg04432 vs. ExPASy TrEMBL
Match: A0A6J1K2S4 (protease Do-like 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111489550 PE=3 SV=1)

HSP 1 Score: 782.7 bits (2020), Expect = 8.0e-223
Identity = 406/442 (91.86%), Postives = 415/442 (93.89%), Query Frame = 0


Query: 1   MAAAFSLASTFFHFSPLSRPPNGRPTSFFLSKSIPFHNFSNPSYHFRYPIFSLLGHNKKR 60
           MAAAFSLASTF HFSP SRPPN  PTSFFL KSI   NF++PS H RYPI SLL HNK  
Sbjct: 1   MAAAFSLASTFSHFSPPSRPPNRTPTSFFLPKSISLQNFTDPSSHLRYPICSLLPHNKNH 60

Query: 61  NQISATTDTKLANPSNPLASICESLLIFTTSVFLSFSLFVTDVDPAAAFVVTTPRKLQTD 120
            QISATT TKL  PSNP ASI ESLLIF+TS  LSFSLF+TDV+PA AFVVTTPRKLQTD
Sbjct: 61  TQISATTHTKLPTPSNPFASIWESLLIFSTSALLSFSLFLTDVEPAVAFVVTTPRKLQTD 120

Query: 121 ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRG 180
           ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDG++VTNYHVIRG
Sbjct: 121 ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGHVVTNYHVIRG 180

Query: 181 ASDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIG 240
           ASDLRVTLADQTTFDAKVVGFDQDKDVAVL IDAPKDKLRPIPVGISADLLVGQKVFAIG
Sbjct: 181 ASDLRVTLADQTTFDAKVVGFDQDKDVAVLSIDAPKDKLRPIPVGISADLLVGQKVFAIG 240

Query: 241 NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN 300
           NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN
Sbjct: 241 NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN 300

Query: 301 TAIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVEQLGVSGVLV 360
           TAIYSPSGASSGVGFSIPVDTVSGIVDQLVR+GKVTRPILGIKFAPDQSVEQLGVSGVLV
Sbjct: 301 TAIYSPSGASSGVGFSIPVDTVSGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLV 360

Query: 361 LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVANGSDLYRILDQCKVGDKVIV 420
           LDAPANGPAGKAGL PTKRDAYGRLILGDIITSV+GKKV NGSDLYRILDQCKVGDKV V
Sbjct: 361 LDAPANGPAGKAGLRPTKRDAYGRLILGDIITSVDGKKVMNGSDLYRILDQCKVGDKVTV 420

Query: 421 EVLRGDHMEKIPVILEPKPDES 443
           EVLRGDHMEKIPVILEPKPDES
Sbjct: 421 EVLRGDHMEKIPVILEPKPDES 442

[Close]

BLAST of Carg04432 vs. ExPASy TrEMBL
Match: A0A6J1BXV6 (protease Do-like 1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111006463 PE=3 SV=1)

HSP 1 Score: 782.3 bits (2019), Expect = 1.0e-222
Identity = 407/442 (92.08%), Postives = 419/442 (94.80%), Query Frame = 0


Query: 1   MAAAFSLASTFFHFSPLSRPPNGRPTSFFLSKSIPFHNFSNPSYHFRYPIFSLLGHNKKR 60
           MAAA+SLASTFFHFSPLSRPPN R T+F L KSI  HNFSNP+ H R+PIFSLL   KK 
Sbjct: 1   MAAAYSLASTFFHFSPLSRPPNRRSTAFLLPKSISLHNFSNPTCHLRFPIFSLL---KKN 60

Query: 61  NQISATTDTKLANPSNPLASICESLLIFTTSVFLSFSLFVTDVDPAAAFVVTTPRKLQTD 120
           +QISAT   KL  PSNP ASI ESLLIF+TSV LSFSLFVTDVDPAAAFVVTTPRKLQTD
Sbjct: 61  SQISATNAPKLTIPSNPFASIWESLLIFSTSVLLSFSLFVTDVDPAAAFVVTTPRKLQTD 120

Query: 121 ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRG 180
           ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDG+IVTNYHVIRG
Sbjct: 121 ELATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGHIVTNYHVIRG 180

Query: 181 ASDLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIG 240
           ASDLRVTLADQTT+DAKVVGFDQDKDVAVLRI+APKDKLRPIPVGISADLLVGQKVFAIG
Sbjct: 181 ASDLRVTLADQTTYDAKVVGFDQDKDVAVLRIEAPKDKLRPIPVGISADLLVGQKVFAIG 240

Query: 241 NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN 300
           NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN
Sbjct: 241 NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN 300

Query: 301 TAIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVEQLGVSGVLV 360
           TAIYSPSGASSGVGFSIPVDTVSGIV+QLV++GKVTRPILGIKFAPDQSVEQLGVSGVLV
Sbjct: 301 TAIYSPSGASSGVGFSIPVDTVSGIVEQLVQFGKVTRPILGIKFAPDQSVEQLGVSGVLV 360

Query: 361 LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVANGSDLYRILDQCKVGDKVIV 420
           LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKV NGSDLYRILDQCKVGDKV V
Sbjct: 361 LDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVTNGSDLYRILDQCKVGDKVTV 420

Query: 421 EVLRGDHMEKIPVILEPKPDES 443
           EVLRGDHMEKIPV LEPKPDES
Sbjct: 421 EVLRGDHMEKIPVTLEPKPDES 439

[Close]

BLAST of Carg04432 vs. TAIR 10
Match: AT3G27925.1 (DegP protease 1 )

HSP 1 Score: 624.8 bits (1610), Expect = 5.4e-179
Identity = 345/440 (78.41%), Postives = 366/440 (83.18%), Query Frame = 0


Query: 7   LASTFFHFSPLSRPPNGRPTSFFL--SKSIPFHNFSNPSYHFRYPIFSLLGHNKKRNQIS 66
           L+ST F    L  PP+   + F L  S+S P   +   S  + + I S L  N   N   
Sbjct: 11  LSSTLF----LHSPPSSHLSFFNLSSSRSSPISLYPIRSKRY-FRILSKLSLND--NNRD 70

Query: 67  ATTDTKLANPSNPLASICESLLIFTTSVFLSFSLFVTD--VDPAAAFVVTTPRKLQTDEL 126
              DT    P     S  +   +  TSV LSFSLF     V+ A+AFVV+TP+KLQTDEL
Sbjct: 71  DDDDTLHFTP----FSAVKPFFLLCTSVALSFSLFAASPAVESASAFVVSTPKKLQTDEL 130

Query: 127 ATVRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRGAS 186
           ATVRLFQENTPSVVYITNLA RQDAFTLDVLEVPQGSGSGFVWDK G+IVTNYHVIRGAS
Sbjct: 131 ATVRLFQENTPSVVYITNLAVRQDAFTLDVLEVPQGSGSGFVWDKQGHIVTNYHVIRGAS 190

Query: 187 DLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIGNP 246
           DLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPK+KLRPIPVG+SADLLVGQKVFAIGNP
Sbjct: 191 DLRVTLADQTTFDAKVVGFDQDKDVAVLRIDAPKNKLRPIPVGVSADLLVGQKVFAIGNP 250

Query: 247 FGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGINTA 306
           FGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSG LIGINTA
Sbjct: 251 FGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGTLIGINTA 310

Query: 307 IYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVEQLGVSGVLVLD 366
           IYSPSGASSGVGFSIPVDTV GIVDQLVR+GKVTRPILGIKFAPDQSVEQLGVSGVLVLD
Sbjct: 311 IYSPSGASSGVGFSIPVDTVGGIVDQLVRFGKVTRPILGIKFAPDQSVEQLGVSGVLVLD 370

Query: 367 APANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVANGSDLYRILDQCKVGDKVIVEV 426
           AP +GPAGKAGL  TKRD YGRL+LGDIITSVNG KV+NGSDLYRILDQCKVGD+V VEV
Sbjct: 371 APPSGPAGKAGLQSTKRDGYGRLVLGDIITSVNGTKVSNGSDLYRILDQCKVGDEVTVEV 430

Query: 427 LRGDHMEKIPVILEPKPDES 443
           LRGDH EKI V LEPKPDES
Sbjct: 431 LRGDHKEKISVTLEPKPDES 439

[Close]

BLAST of Carg04432 vs. TAIR 10
Match: AT5G39830.1 (Trypsin family protein with PDZ domain )

HSP 1 Score: 299.3 bits (765), Expect = 5.2e-81
Identity = 169/328 (51.52%), Postives = 215/328 (65.55%), Query Frame = 0


Query: 125 VRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRGASDL 184
           V+LF++NT SVV I ++  R       V+E+P+G+GSG VWD  G IVTNYHVI  A   
Sbjct: 120 VQLFEKNTYSVVNIFDVTLRPQLKMTGVVEIPEGNGSGVVWDGQGYIVTNYHVIGNALSR 179

Query: 185 RVTLAD-------------QTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLL 244
             +  D             Q  F+ K+VG D+ KD+AVL++DAP+  L+PI VG S  L 
Sbjct: 180 NPSPGDVVGRVNILASDGVQKNFEGKLVGADRAKDLAVLKVDAPETLLKPIKVGQSNSLK 239

Query: 245 VGQKVFAIGNPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLD 304
           VGQ+  AIGNPFG DHTLT GVISGL R+I S  TG  I   IQTDAAINPGNSGGPLLD
Sbjct: 240 VGQQCLAIGNPFGFDHTLTVGVISGLNRDIFS-QTGVTIGGGIQTDAAINPGNSGGPLLD 299

Query: 305 SSGNLIGINTAIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVE 364
           S GNLIGINTAI++ +G S+GVGF+IP  TV  IV QL+++ KV R  + I+ APD    
Sbjct: 300 SKGNLIGINTAIFTQTGTSAGVGFAIPSSTVLKIVPQLIQFSKVLRAGINIELAPDPVAN 359

Query: 365 QLGV-SGVLVLDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVANGSDLYRILD 424
           QL V +G LVL  P    A KAGL PT R   G ++LGDII +V+ K V N ++L +ILD
Sbjct: 360 QLNVRNGALVLQVPGKSLAEKAGLHPTSRGFAGNIVLGDIIVAVDDKPVKNKAELMKILD 419

Query: 425 QCKVGDKVIVEVLRGDHMEKIPVILEPK 439
           +  VGDKV +++ RG+   ++ + LE K
Sbjct: 420 EYSVGDKVTLKIKRGNEDLELKISLEEK 446

[Close]

BLAST of Carg04432 vs. TAIR 10
Match: AT5G39830.2 (Trypsin family protein with PDZ domain )

HSP 1 Score: 274.6 bits (701), Expect = 1.4e-73
Identity = 161/328 (49.09%), Postives = 204/328 (62.20%), Query Frame = 0


Query: 125 VRLFQENTPSVVYITNLAARQDAFTLDVLEVPQGSGSGFVWDKDGNIVTNYHVIRGASDL 184
           V+LF++NT SVV I ++  R       V+E+P+G+GSG VWD  G IVTNYHVI  A   
Sbjct: 120 VQLFEKNTYSVVNIFDVTLRPQLKMTGVVEIPEGNGSGVVWDGQGYIVTNYHVIGNALSR 179

Query: 185 RVTLAD-------------QTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLL 244
             +  D             Q  F+ K+VG D+ KD+AVL++DAP+  L+PI VG S  L 
Sbjct: 180 NPSPGDVVGRVNILASDGVQKNFEGKLVGADRAKDLAVLKVDAPETLLKPIKVGQSNSLK 239

Query: 245 VGQKVFAIGNPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLD 304
           VGQ+  AIGNPFG DHTLT GVISGL R+I S  TG  I   IQTDAAINPGNSGGPLLD
Sbjct: 240 VGQQCLAIGNPFGFDHTLTVGVISGLNRDIFS-QTGVTIGGGIQTDAAINPGNSGGPLLD 299

Query: 305 SSGNLIGINTAIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAPDQSVE 364
           S GNLIGINTAI++               TV  IV QL+++ KV R  + I+ APD    
Sbjct: 300 SKGNLIGINTAIFT--------------QTVLKIVPQLIQFSKVLRAGINIELAPDPVAN 359

Query: 365 QLGV-SGVLVLDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKVANGSDLYRILD 424
           QL V +G LVL  P    A KAGL PT R   G ++LGDII +V+ K V N ++L +ILD
Sbjct: 360 QLNVRNGALVLQVPGKSLAEKAGLHPTSRGFAGNIVLGDIIVAVDDKPVKNKAELMKILD 419

Query: 425 QCKVGDKVIVEVLRGDHMEKIPVILEPK 439
           +  VGDKV +++ RG+   ++ + LE K
Sbjct: 420 EYSVGDKVTLKIKRGNEDLELKISLEEK 432

[Close]

BLAST of Carg04432 vs. TAIR 10
Match: AT4G18370.1 (DEGP protease 5 )

HSP 1 Score: 196.8 bits (499), Expect = 3.6e-50
Identity = 118/239 (49.37%), Postives = 152/239 (63.60%), Query Frame = 0


Query: 116 KLQTDELATVRLFQENTPSVVYITNL----AARQDAFTLDVLEVPQGSGSGFVWDKDGNI 175
           +L+ +E   V LFQ+ +PSVVYI  +     +  D  T +     +G+GSGFVWDK G+I
Sbjct: 83  ELEEEEERNVNLFQKTSPSVVYIEAIELPKTSSGDILTDEENGKIEGTGSGFVWDKLGHI 142

Query: 176 VTNYHVIR-------GASDLRVTLADQ--TTF--DAKVVGFDQDKDVAVLRIDAPKDKLR 235
           VTNYHVI        G    +V+L D   T F  + K+VG D D D+AVL+I+    +L 
Sbjct: 143 VTNYHVIAKLATDQFGLQRCKVSLVDAKGTRFSKEGKIVGLDPDNDLAVLKIETEGRELN 202

Query: 236 PIPVGISADLLVGQKVFAIGNPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAI 295
           P+ +G S DL VGQ  FAIGNP+G ++TLT GV+SGL REI S   G+ I + IQTDA I
Sbjct: 203 PVVLGTSNDLRVGQSCFAIGNPYGYENTLTIGVVSGLGREIPS-PNGKSISEAIQTDADI 262

Query: 296 NPGNSGGPLLDSSGNLIGINTAIYS--PSGASSGVGFSIPVDTVSGIVDQLVRYGKVTR 338
           N GNSGGPLLDS G+ IG+NTA ++   SG SSGV F+IP+DTV   V  L+ YG   R
Sbjct: 263 NSGNSGGPLLDSYGHTIGVNTATFTRKGSGMSSGVNFAIPIDTVVRTVPYLIVYGTAYR 320

[Close]

BLAST of Carg04432 vs. TAIR 10
Match: AT5G27660.1 (Trypsin family protein with PDZ domain )

HSP 1 Score: 137.1 bits (344), Expect = 3.4e-32
Identity = 100/290 (34.48%), Postives = 146/290 (50.34%), Query Frame = 0


Query: 138 ITNLAARQDAFTLDVLEVPQG---------SGSGFVWDKDGNIVTNYHVIRGASDLR--- 197
           I N AAR     ++ L VPQG          GSG + D DG I+T  HV+    ++R   
Sbjct: 119 IANAAARIGPAVVN-LSVPQGFHGISMGKSIGSGTIIDADGTILTCAHVVVDFQNIRHSS 178

Query: 198 -----VTLADQTTFDAKVVGFDQDKDVAVLRIDAPKDKLRPIPVGISADLLVGQKVFAIG 257
                VTL D  TF+  VV  D   D+A+++I + K  L    +G S+ L  G  V A+G
Sbjct: 179 KGRVDVTLQDGRTFEGVVVNADLQSDIALVKIKS-KTPLPTAKLGFSSKLRPGDWVIAVG 238

Query: 258 NPFGLDHTLTTGVISGLRREISSAATGRPIQDVIQTDAAINPGNSGGPLLDSSGNLIGIN 317
            P  L +T+T G++S + R+ S    G   ++ +QTD +IN GNSGGPL++  G +IG+N
Sbjct: 239 CPLSLQNTVTAGIVSCVDRKSSDLGLGGKHREYLQTDCSINAGNSGGPLVNLDGEVIGVN 298

Query: 318 TAIYSPSGASSGVGFSIPVDTVSGIVDQLVRYGKVTRPILGIKFAP--DQSVEQLG---- 377
                   A+ G+GFS+P+D+VS I++   + G+V RP +G+K     +  V QL     
Sbjct: 299 IMKVL---AADGLGFSVPIDSVSKIIEHFKKSGRVIRPWIGLKMVELNNLIVAQLKERDP 358

Query: 378 -----VSGVLVLDAPANGPAGKAGLLPTKRDAYGRLILGDIITSVNGKKV 400
                  GVLV       PA +AG  P           GD++   +GK V
Sbjct: 359 MFPDVERGVLVPTVIPGSPADRAGFKP-----------GDVVVRFDGKPV 392

The following BLAST results are available for this feature:

BLAST of Carg04432 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7033121.1	2.1e-246	100.00	Protease Do-like 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022922573.1	6.2e-246	99.77	protease Do-like 1, chloroplastic [Cucurbita moschata] >KAG6602439.1 Protease Do...	[more]
XP_023515810.1	3.4e-244	99.10	protease Do-like 1, chloroplastic [Cucurbita pepo subsp. pepo]	[more]
XP_022990583.1	8.4e-243	98.64	protease Do-like 1, chloroplastic [Cucurbita maxima]	[more]
XP_023550169.1	6.7e-224	92.53	protease Do-like 1, chloroplastic [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg04432 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22609	7.5e-178	78.41	Protease Do-like 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP1 PE=1 ...	[more]
Q9LU10	7.3e-80	51.52	Protease Do-like 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DEGP8 PE=1 ...	[more]
Q2YMX6	8.4e-52	41.30	Probable periplasmic serine endoprotease DegP-like OS=Brucella abortus (strain 2...	[more]
P0C114	8.4e-52	41.30	Probable periplasmic serine endoprotease DegP-like OS=Brucella abortus biovar 1 ...	[more]
Q8YG32	8.4e-52	41.30	Probable periplasmic serine endoprotease DegP-like OS=Brucella melitensis biotyp...	[more]

BLAST of Carg04432 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E723	3.0e-246	99.77	protease Do-like 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111430538 ...	[more]
A0A6J1JTP9	4.1e-243	98.64	protease Do-like 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111487418 PE...	[more]
A0A6J1FEU2	4.7e-223	92.08	protease Do-like 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111445066 ...	[more]
A0A6J1K2S4	8.0e-223	91.86	protease Do-like 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111489550 PE...	[more]
A0A6J1BXV6	1.0e-222	92.08	protease Do-like 1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111006463...	[more]

BLAST of Carg04432 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G27925.1	5.4e-179	78.41	DegP protease 1	[more]
AT5G39830.1	5.2e-81	51.52	Trypsin family protein with PDZ domain	[more]
AT5G39830.2	1.4e-73	49.09	Trypsin family protein with PDZ domain	[more]
AT4G18370.1	3.6e-50	49.37	DEGP protease 5	[more]
AT5G27660.1	3.4e-32	34.48	Trypsin family protein with PDZ domain	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001940	Peptidase S1C	PRINTS	PR00834	PROTEASES2C	coord: 190..210 score: 43.55 coord: 270..287 score: 72.49 coord: 292..309 score: 52.85 coord: 232..256 score: 55.41 coord: 384..396 score: 44.84 coord: 169..181 score: 58.54
IPR001478	PDZ domain	SMART	SM00228	pdz_new	coord: 338..426 e-value: 5.9E-8 score: 42.4
IPR001478	PDZ domain	PFAM	PF13180	PDZ_2	coord: 338..435 e-value: 2.9E-13 score: 50.1
IPR001478	PDZ domain	PROSITE	PS50106	PDZ	coord: 329..401 score: 10.4559
IPR043504	Peptidase S1, PA clan, chymotrypsin-like fold	GENE3D	2.40.10.10		coord: 106..222 e-value: 2.1E-38 score: 132.5
IPR043504	Peptidase S1, PA clan, chymotrypsin-like fold	GENE3D	2.40.10.10		coord: 223..337 e-value: 1.1E-41 score: 143.5
None	No IPR available	PFAM	PF13365	Trypsin_2	coord: 160..299 e-value: 1.1E-34 score: 120.6
None	No IPR available	PANTHER	PTHR43019	SERINE ENDOPROTEASE DEGS	coord: 58..442
None	No IPR available	PANTHER	PTHR43019:SF38	PROTEASE DO-LIKE 1, CHLOROPLASTIC	coord: 58..442
None	No IPR available	CDD	cd00987	PDZ_serine_protease	coord: 338..433 e-value: 1.3468E-17 score: 75.3688
IPR036034	PDZ superfamily	GENE3D	2.30.42.10		coord: 338..442 e-value: 7.5E-39 score: 133.7
IPR036034	PDZ superfamily	SUPERFAMILY	50156	PDZ domain-like	coord: 296..426
IPR009003	Peptidase S1, PA clan	SUPERFAMILY	50494	Trypsin-like serine proteases	coord: 125..335

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg04432-RA	Carg04432-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0043231	intracellular membrane-bounded organelle
molecular_function	GO:0005515	protein binding
molecular_function	GO:0004252	serine-type endopeptidase activity